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PAKISTAN INTERNATIONAL SCHOOL, JEDDAH – ES

ACADEMIC SESSION 2023 - 2024

TEST 3 - V1
STUDENT Std. ID
NAME

CLASS/SEC A 2s - DATE

BIOLOGY 9700
40 minutes
You must answer on the Question Paper.

No additional materials are needed.

INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, class/sec and date in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● You may use a calculator.
● You should show all your working and use appropriate units.

INFORMATION
● The total mark for this paper is 30.
● The number of marks for each question or part question is shown in brackets [ ].

Total

This document consists of 9 pages.

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Section 1 - Structured Questions

Total Marks: 19
Answer all questions.

1 Phloem sap is transported from sources to sinks in phloem sieve tubes. Each sieve tube is
constructed from phloem sieve tube elements.

(a) The structure of a phloem sieve tube element is adapted to its function.

Each of explanations A to F describes how a particular structural feature of a phloem sieve

tube element in a source is suited to the function of transporting phloem sap.

The matching structural feature for each explanation is listed in Table 1.1.

A for entry of sucrose and other organic compounds

B for rapid entry of water to create high hydrostatic pressure

C provides pores to allow the flow of phloem sap from one sieve tube element to the next

D to form very long tubular structures for the transport of phloem sap from source to sink

E decreases resistance to the flow of phloem sap within each sieve tube element, so the
speed of flow is maintained

F provides more space to increase the volume of phloem sap transported per unit time

Complete Table 1.1 by writing the correct letter from A to F in the last column of each row, so
that each structural feature is matched to the correct explanation.

Use each letter only once.

Table 1.1

structural feature of phloem sieve tube element explanation

There is no nucleus or large permanent vacuole.

The end walls are perforated to form sieve plates.

There is only a thin layer of cytoplasm around the edge of the cell.

The cell is elongated and arranged end to end with other cells.

The cell has plasmodesmata connecting to a companion cell.

There is a thin cellulose cell wall.

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(b) At the sink, sucrose and other organic compounds are unloaded from the phloem sieve tube
element.
Explain why the process of unloading helps the mass flow of phloem sap from the source to
the sink.
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(c) Explain why phospholipids are able to form a bilayer in cell membranes.

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(d) The mitotic cell cycle of the stem cells present in the gas exchange system is carefully
controlled. During interphase of the mitotic cell cycle, cells grow by increasing in size.
Complete Table 1.2 by:
• listing, in order, the three phases that occur during interphase
• stating one process, other than growth and respiration, that occurs in each of these
three phases to help prepare the cell for mitosis.
Table 1.2

phase process occurring during phase

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Fig. 1.1 is a transmission electron micrograph of a plant parenchyma cell.

cell sap
in vacuole

cytosol
(fluid part of
cytoplasm) Y tonoplast

Fig. 1.1

The cell sap in the vacuole of the cell shown in Fig. 1.1 has a pH of 5.0. The cytosol has a pH
of 7.2.

The tonoplast controls the passage of hydrogen ions from the cytosol into the vacuole. The
low pH created by the entry of hydrogen ions is optimum for the action of acid hydrolase
enzymes in the vacuole. Acid hydrolase enzymes are also found in lysosomes in animal cells.

(e) Suggest which transport mechanism is used to move hydrogen ions from the cytosol of
the parenchyma cell into the vacuole.

Explain your choice.

transport mechanism .........................................................................................................

explanation ........................................................................................................................

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[Total: 19]
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Section 2 - Multiple Choice Questions

Total Marks: 11
Note: Mark all your answers on given answer sheet on the last page.

1 Which description of cell surface membrane permeability is correct?

A An increase in the concentration of cholesterol molecules in the cell surface membrane can
increase its permeability to hydrophilic substances.

B Cell surface membrane permeability to large hydrophilic molecules is high and can be
increased by membrane transport proteins involved in facilitated diffusion.

C The permeability of the cell surface membrane to ions increases as the proportion of
saturated fatty acid chains in the phospholipids increases.
D Without the presence of carrier and channel membrane proteins, the cell surface membrane
has a low permeability to large polar molecules.

2 Which transport mechanism within a cell can occur in the absence of membranes?

A active transport

B diffusion
C facilitated diffusion

D osmosis

3 A student measured the time taken for complete diffusion of a dye into agar blocks of different
sizes.

The results are shown in the table.

size of agar block time for

/ mm × mm × mm diffusion / s

5 × 5× 5 6.2
10 × 10 × 10 16.1
15 × 15 × 15 34.5
5 × 10 × 15

What is the predicted time for complete diffusion of the dye into the agar block measuring
5 mm × 10 mm × 15 mm?

A 6.2 s
B 16.1 s

C 34.5 s
D more than 34.5 s

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4 A plant cell with a water potential of –600 kPa was placed in a solution with a water potential of
–410 kPa for 10 minutes.

Which row is correct?

net movement water potential

effect on cell
of water of cell

A into cell becomes higher becomes turgid

B into cell becomes lower bursts
C out of cell becomes higher swells
D out of cell becomes lower becomes plasmolysed

5 In which stage of the cell cycle are telomeres needed to prevent the loss of genes?

A prophase

B cytokinesis

C G1 phase
D S phase

6 Which row shows part of the correct sequence of mitosis?

A chromosomes nuclear
chromosomes spindle fibres
→ line up at the → envelope →
condense shorten
equator disappears

B chromosomes chromosomes nuclear

spindle fibres
line up at the → shorten → condense → envelope
equator reappears
C chromosomes nuclear
spindle fibres chromosomes
line up at the → shorten → envelope → uncoil
equator reappears

D nuclear chromosomes
chromosomes spindle fibres
uncoil → envelope → shorten → line up at the
disappears equator
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7 The photomicrograph shows a section of a plant organ.

Students were asked to study the photomicrograph and identify the organ, giving a reason to
support their answer.

Which statement written by the students correctly identifies the organ and provides a correct
reason to support this identification?

A It is a leaf because the xylem is located on the inner side of each vascular bundle.
B It is a stem because there is a ring of endodermis visible around the edge of the vascular
tissue.
C It is a stem because the vascular bundles are arranged in a ring near the epidermis.
D It is a xerophytic leaf because there is a thick epidermis made up of many layers of cells.

8 What is correct about the apoplastic pathway and symplastic pathway in a root?

apoplastic pathway symplastic pathway

A Casparian strip blocks water moves across the
the movement of water root through cell walls
B water moves across the water moves from cell to
root through cell walls cell via the plasmodesmata
C water moves from cell to water passes through
cell via the plasmodesmata the cytoplasm
D water passes through Casparian strip blocks
the cytoplasm the movement of water

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9 Sucrose moves into a phloem sieve tube element from a leaf mesophyll cell.
Which changes to the water potential and the volume of solution in the phloem sieve tube
element are correct?

water potential volume of

becomes solution

A higher decreases
B higher increases
C lower decreases
D lower increases

10 The diagram shows an eyepiece graticule and cell viewed through a microscope. When the
eyepiece graticule was calibrated at this magnification, the whole length of the graticule shown
covered 12 divisions of a stage micrometer scale.
There were 100 divisions in 10 mm of the stage micrometer.

0 10 20 30 40 50 60 70 80 90 100

What is the actual length of the cell?

A 2.5 µm B 3.6 µm C 360 µm D 3 mm

11 The molecule shown is a polymer of reducing sugars.

CH2OH CH 2OH CH 2OH CH2OH

O O O O

OH OH OH OH
O O O
HO OH
OH OH OH OH

Which procedures could be carried out to show that this molecule is a polymer of reducing
sugars?

1 Add hydrolytic enzyme and then heat with Benedict’s solution.

2 Dissolve in water, neutralise and then heat with Benedict’s solution.
3 Boil with hydrochloric acid, neutralise and then heat with Benedict’s solution.

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

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Multiple choice answer sheet