Act01 The Microscope Exercise

Activity #1
THE MICROSCOPE
Student’s Name GIANAN, JEANNE FAITH V. Date Performed OCTOBER

Course/Year/Section BIO-SEC/2021/NO1A 20,2021
Instructor’s Name PROF.LEANO, BRYNA
Date Submitted OCTOBER
THEZZA D.
24,2021
I. A. Label the parts of the microscope
EYEPIECE
NOSEPIECE
OBJECTIVES
STAGE CLIPS
MECHANICAL STAGE
LIGHT SOURCE
FIELD DIAPHRAGM
COARSE FOCUS
FINE FOCUS
Compound Light Microscope

B. Magnification
Eyepiece X Objective = Total Magnification
Scanner 10x x 4x = 40x

LPO 10x x 10x = 100x
HPO 10x x 40x = 400x
Oil immersion 10x x 100x = 1000x
C. Draw letter "e"
1. Normal position
2. Under low-power objective

3. Under high-power objective
D. The Ocular Micrometer
1. 1. Compute for the calibration constant of your ocular micrometer. Show

your solution.
Under LPO:
1 ocular Div. x 1000μm = 10 ocular Div. x ? μm

10 ocular Div. 10 ocular Div.
=1 ocular Div. x 1000μm =? mm = 100μm

10 ocular Div.
Under HPO:
1 ocular Div. x 1000 μm = 40 ocular Div. x ? μm

40 ocular Div. 40 ocular Div.
=1 ocular Div. x 1000 μm =? mm = 25μm

40 ocular Div.
1.2. How do these compare with each other?

These two compares by having difference in its size of how it is viewed in the
microscope. In the LPO the size is larger however it is not as zoomed, in
comparison to the HPO because of its difference in magnification.
2.1. Determine the width (in μm) of the Paramecium (same area). Show your
solution.
Using LPO:
19 Div. x 100mm = 190μm
Using HPO:
77 Div. x 25mm =1,925μm
2. 2. How do these compare with each other?
These two compares in terms of with LPO, the Paramecium can be seen only as
a small fraction of a size compared to the HPO which can be in seen in a larger
size and has a more precise detail.
II. Guide Questions:
1. What is the correct procedure for carrying the microscope?

The correct procedure is to first carry it with two hands one hand, handles the
arm of the microscope while the other is at the base. Second is to make sure
that the microscope is facing away from you and the electrical cord is secured
and in place, make sure that the microscope is cleaned, the stage is lowered,
and the objectives are rotated properly in position for it to be stored in the
cabinet.
2. Compare the image seen in the compound microscope and dissecting

microscope in terms of magnification, dimensions, inversion of image and
direction of movement. Tabulate your answers.
COIN 1 COIN 2 COIN 3
The coin can be seen The coin can be seen Out of the three
1. MAGNIFICATION closely due to the more closely and has pictures this, does not
magnification finer details, you can provide with clearer
applied, there are see that it appears to details despite its
certain details that have more scratches higher magnification
are seen finely and is more textured level.
especially the than the previous Some details are seen
dimension of the picture. quite well but does
word “PISO” as well not emphasize the
as texture like the texture of the coin.
scratches in the coin.
As you can see the As you can see the As you can see the
2. DIMENSIONS coin looks quite big coin looks bigger coin looks the biggest
than it around 50% than it is around 75% it can be since only
bigger assumably bigger assumably the “PISO” word is
around that around that almost the only one
percentage. percentage. Since that fits through the
the word “PISO” can lens. It looks like it is
be seen in larger size double the size than it
and in details. is.
The image is not The image is not The image is not

3. INVERSION OF inverted but can inverted but can inverted but can
IMAGE rather be seen its rather be seen its rather be seen its
normal position. normal position. normal position.
Since it is tilted to the Since it is tilted to Since it is tilted to the
4. DIRECTION OF right its direction of the left its direction left its direction of
MOVEMENT movement is to the of movement is to movement is to the
left. the right right
3. How does one improve contrast when using a dissecting microscope?
The contrast can be improved by adjusting the coarse and fine focus which can
give the specimen a much clearer emphasis and by adjusting the light source,
that is used to collect and focus light from the illuminator into the specimen. So
that the specimen can have a better contrast and detailed viewpoint.

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Activity #1

Student’s Name GIANAN, JEANNE FAITH V. Date Performed OCTOBER

I. A. Label the parts of the microscope

Compound Light Microscope

Eyepiece X Objective = Total Magnification

Scanner 10x x 4x = 40x

C. Draw letter "e"

2. Under low-power objective

D. The Ocular Micrometer

1. 1. Compute for the calibration constant of your ocular micrometer. Show

1 ocular Div. x 1000μm = 10 ocular Div. x ? μm

=1 ocular Div. x 1000μm =? mm = 100μm

1 ocular Div. x 1000 μm = 40 ocular Div. x ? μm

=1 ocular Div. x 1000 μm =? mm = 25μm

1.2. How do these compare with each other?

19 Div. x 100mm = 190μm

77 Div. x 25mm =1,925μm

2. 2. How do these compare with each other?

1. What is the correct procedure for carrying the microscope?

2. Compare the image seen in the compound microscope and dissecting

COIN 1 COIN 2 COIN 3

The image is not The image is not The image is not

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