Zoology

B.Sc.
(vi) –SEMESTER I – Part III a MAJOR

ANIMAL LIFE I 6 Hours / week 5 Credits
(for students admitted from 2008-’09 onwards)
UNIT I
Introduction to Animal Kingdom. Outline classification, major hierarchies. Definition of
Taxonomy, systematics, classification, identification, nomenclature, International code of
Zoological Nomenclature & International Congress of Zoology and International Commission on
Zoological Nomenclature
UNIT II
Phylum : Protozoa
General characters and classification up to the classes with examples. Type Study: Plasmodium
sp. Life cycle, different species, symptoms, health care, control measures. Protozoan parasites and
disease: Leishmania, Entamoeba, Trypanosoma, Balantidium.
UNIT III
Phylum: Porifera
General characters and classification upto the classes with examples.
Type Study: Sycon sp. Reproduction of sponges, Gemmules, Canal system of sponges.
UNIT IV
Phylum: Coelenterata
Type Study: Aurelia sp.
Corals and coral reefs.
UNIT V
Phylum: Platyhelminthes
Type Study: Taenia solium
Common Cestode and Trematode parasites of man.
UNIT VI
Phylum: Aschelminthes
General characters and classification upto classes with examples.
Type Study: Wuchereria bancrofti
Common nematode parasites of man.
UNIT VII
Phylum: Annelida
General characters and classification upto classes with examples.
Type Study: Megascolex sp.
Vermiculture.
UNIT VIII
Phylum: Arthropoda
General characters and outline classification
Type Study: Periplaneta sp.
Economic importance of insects in relation to agriculture and human health.
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UNIT IX
Phylum: Mollusca
General characters and classification with examples.
Type Study: Pila sp. Pearl culture
UNIT X
Phylum : Echinodermata
General characters and classification with examples
Type Study: Asterias
Water - vascular system, Larval forms of Echinodermata.
UNIT XI
Phylum: Hemichordata
General characters and classification with examples.
Type Study: Balanoglossus sp. Phylogenetic significance.
Reference Books:
Hickman, Jr.C.P. 1981 Integrated Principles of Zoology 7th Ed.

Terera Andesirk and Gerald A 1990 Biology Life of earth
Ayyar, E.A., Manual of Zoology Part I
Jordan E.L. 2004 Invertebrate Zoology Chand & Co
Jordan and Verma 2006 Invertebrate Zoology, Chand & Co
Kotpal series, Rastogi Publication
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B.Sc. (vi) - SEMESTER I – PART III a MAJOR
MICROBIOLOGY 4 Hours / week 5 Credits
Objective: To expose the students to various micro-organisms and their applied aspects.
UNIT I
History of Microbiology
Pasteur, Lister, Alexander Flemming - General Classification of microorganisms - Salient features
of bacteria, viruses, algae, fungi, and yeast.
UNIT II
Structure of the Bacterial cell - cell wall - Gram +ve and – ve.
Bacterial photosynthesis, Respiration - aerobic and anaerobic, Fermentation/ nutrition and
growth. Reproduction, genetic transfer system in microbes.
UNIT III
Industrial Microbiology: Microbes in the production of alcohol and antibiotics.
Dairy microbiology: Microbiology of milk and milk products, milk-borne diseases.
Agricultural microbiology: Nitrogen cycle, phosphorous cycle, nitrogen fixation, soil fertility and
biogas.
UNIT IV
Microbiology of Drinking water.
Methodology of bacterial analysis of water - Waterborne diseases.- Sewage - biological sewage
purification methods. Chlorination of water and its implication – Trihalomethane (THM).
UNIT V
Medical Microbiology – study of common bacterial (10), fungal (5) and viral (5) diseases of man
and their preventive measures.
Reference Books:
Microbiology – Anna K. Joshua
Microbiology – Norten C.F.
Microbiology – Pelcazar, Reid and Chan
Text Book of Microbiology – Ananthanarayanan and Jayaram
Food Microbiology – Frazier
Industrial Microbiology – Casida L.F
Microbiology of water and Sewage-Geinyl and Lord
General Microbiology – Boyd
3
Microbiology – Atlas – Biology of Microorganisms – Brock and Madigan
General Microbiology – Stainer, John, Mark
Microbiology – Zinsser
B.Sc (vi) - SEMESTER I: Part III c. ALLIED I

ANCILLARY ZOOLOGY –I 4 Hours/ week 3 Credits
Invertebrata
UNIT – I
Outline Classification of Animal Kingdom.
UNIT – II
Protozoa: General characters, Classification upto classes, Life history, Mode of infection &
preventive measures of Entamoeba histolytica, Trypanosoma gambiense, Plasmodium vivax.
UNIT – III
Porifera: General characters, Classification upto classes, General organization of a simple sponge,
spicules, Parenchymula, Amphiblastula, Gemmule, canal systems, Economic importance of
sponges.
UNIT – IV
Coelenterata: General characters, Classification upto classes, General organization of sea
anemone, Basic knowledge about polymorphism, coral reef.
UNIT – V
Platyhelminthes: General characters, Classification upto classes, structure, life history, Mode of
infection of Fasciola hepatica, parasitic adaptations, Platyhelminth parasites of man.
UNIT – VI
Aschelminthes: General characters, Aschelminthic parasites of man and their significance.
UNIT – VII
Annelida: General characters, Classification upto classes, Metamerism – Cephalisation.
UNIT – VIII
Arthropoda: General characters, Classification upto classes, Pests of paddy,
brinjal,sugarcane,vectors and household insects (mosquito, housefly, flea, bed bug, termite, silver
fish, carpet beetle, cockroach).
Commercial importance of Insects – Lac culture, Apiculture, Sericulture
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UNIT – IX
Mollusca: General characters, Classification upto classes, Pearl culture. Economic importance of
Molluscs.
UNIT – X
Echinodermata: General characters, Classification upto classes with local examples.
Reference Books:
1. Manual of Zoology – Ekkambaranathan Ayyar and Ananthakrishnan.
B.Sc. (vi) - SEMESTER I & II – PART III c : ALLIED - I

ANCILLARY ZOOLOGY PRACTICAL 2 Hours / week 2 Credits
Observation:
1. Amoeba sp., Euglena sp., Paramecium sp., E. histolytica, Noctiluca sp., Euplectella,
Spongilla sp., Sycon sp., Physalia sp., Aurelia sp., Sea anemone, Fasciola sp.,
Miracidium, Redia, Cercaria, Taenia sp., Scolex and Proglottids of Taenia sp., Lac insect,
Silkworm cocoon, Mytilus sp., Chiton sp., Dentalium sp., Octopus sp., Asteropecten sp.,
Holothuria sp., Salmacis sp.
2. Two major pests of Paddy and Brinjal
3. Four specimens of insects of public health importance
4. Dissections: Cockroach- All systems
5. Mountings: mouth parts of Cockroach, Honey bee, House fly, Mosquito, Honey bee –
antenna cleaner and Pollen basket
6. Field visit to observe the damage caused by pests of paddy and brinjal
Observation:
1. Amphioxus – entire, C.S. through pharynx and intestine
2. 5 Elasmobranchs and 5 Teleosts of South India
3. Exocoetus
4. Parental care in Amphibia (Ichthyophis sp. and Alytes sp.)
5. 5 poisonous reptiles – Cobra (Naja sp.), Krait (Bungarus sp.), Viper (Echis sp.),
Enhydrina sp., Phrynosoma sp.
6. Chameleon
7. 2 Aquatic birds
8. Echidna, Platypus, Bat, Loris
9. Demonstration : Frog, - Digestive system, circulatory system, urinogenital system -
10. Mounting: Placoid scales
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B.Sc. (vi) - SEMESTER I – Part IV a : NON - MAJOR
LIFESTYLE RELATED AILMENTS 4 Hours / week 2 Credits
(for students admitted from 2014- 2015 onwards)
Objective
To enable non-Zoology students to get the basic insights to various functions and malfunctions.
UNIT I: Changing patterns in health foods. Obesity, gall bladder stones, ulcers, structure of the
stomach, role of enzymes. Liver cirrhosis, Fatty liver, regenerative capacity of liver etc. How can
we safeguard the liver?Importance of maintaining blood sugar through production of insulin.
Diabetes mellitus. Role of Pancreas. Can we reduce the diabetic problem?
UNIT II: Understanding the role of stress. Study of the basic structure of heart and its functions.
Understanding bypass, stenting, angioplasty, angina, AF (atrial fibrillation), myocardial infarction
and arteriosclerosis / atherosclerosis, ECG.
UNIT III: Allergies leading to wheezing, asthma-causes, smoking and its ill effects, their
influence on the alveoli of the lung. Pollution through inhalation of carbon monoxide etc.
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UNIT IV: Importance of EEG, Dementia, Alzheimer’s, Parkinson’s, Stroke aneurysm.
UNIT V: Kidney stones, significance of urea, creatinine, electrolytes in blood, urinary infection,
ultra-filtration, kidney transplants, dialysis.
UNIT VI: Concept of fertility and infertility- Male and Female reproductive systems, Impotency,
Endometriosis, ectopic, IVF, ICSU, Surrogacy, STD, Birth control measures etc.
B.Sc. (vi) - SEMESTER II – Part III a MAJOR

ANIMAL LIFE II 6 Hours / week 5 Credits
UNIT I
The Chordates: General characteristics, four chordate hallmarks (dorsal tubular nerve cord, notochord,
gill slits and post anal tail) adaptations that have guided chordate evolution, ancestry and evolution.
General characteristics and classification for subphylum- Urochordata and Cephalochordata.
Type Study: Ascidia, Amphioxus.
UNIT II
Agnatha: Ostracoderms and Cyclostomata – classification and general characteristics.
UNIT III
Gnathostomata : Placoderms – classification and general characteristics.
UNIT IV:
Fishes: Evolution and phylogeny, cartilagenous fishes (Elasmobranchii, Holocephali), Bony fishes
(Teleostomi); Dipnoi, Crossopterygii, Branchiopterygii and Actinopterygii : Chirolepis – chondrostei,
Holostei and Teleostei. Fins and locomotion, swim bladder, accessory respiratory organs, deep sea
fishes, specialized organs: Electric organs, sound producing organs, light producing organs, poison
glands. Migration and parental care. Food fishes of India (10 marine and 10 FW).
UNIT V
Amphibians: Movement onto land – physical contrast between aquatic and land habitats. Origin and
relationships of amphibians, appearance of lungs, development of limbs for travel on land, earliest
amphibians, amphibian contribution to vertebrate evolution. General characteristics and classification
– Coecilians (Apoda) Salamanders and newts (Urodela), frogs and toads (Anura) - parental care.
UNIT VI
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Reptiles: origin and adaptive radiation of extinct reptiles – general characteristics and classification up
to orders with local examples – poisonous and non-poisonous reptiles with special reference to snakes
of India. – Reptiles of Mesozoic Era – Phylogeny of Reptiles
UNIT VII
Birds: Origin and relationship, general characteristics with local examples. Common Birds of India
( any Ten)- Form and function, movement and integration, migration and navigation, Ratitae perching
mechanism, flight adaptations.- Types of beak and feet, nesting and parental care
UNIT VIII
Mammals: General characteristics and Classification up to orders with examples – Dentition in
mammals – Placentation – Flying mammals – Aquatic mammals – Adaptive radiation – Phylogeny –
Human Skeletal System
UNIT IX
Jaw suspension in vertebrates – Aortic arches in vertebrates – Development of brain and kidney in
vertebrates.
Reference Books:
Hickman
E.K. Ayyar
Jordan
J.Z. Young
B.Sc. (vi) - SEMESTER II: Part III a MAJOR

ANIMAL LIFE & MICROBIOLOGY PRACTICAL 2+2 Hours / week 5 credits
Animal Life - Invertebrata

1. Identification and observation of features of taxonomic and economic importance (in relation
to biological, agricultural and health) sketching and systematic position of the following
specimens.
Biological: Paramecium, Euplectella, Aurelia, Heteronereis, Limulus, Honeybee, Chiton,
Sepia.
Agricultural: Earthworm, Bombyx mori, any one pest of paddy and brinjal.
Health: Entamoeba, Taenia solium, Anopheles and Musca domestica.
2. Dissection: Digestive, reproductive and nervous systems of Cockroach/ Grasshopper
3. Study of larval forms: Miracidium, Redia, Cercaria, Nauplius, Glochidium and Pluteus.
4. Mounting: Mouthparts of cockroach – Honey Bee: sting apparatus, pollen basket and
antenna cleaner.
Animal Life - Vertebrata
1. Demonstration of dissections of various systems: Alimentary, circulatory and urinogenital
systems of frog.
2. Mounting: Placoid scales of Shark.
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3. Slides and specimens:
Prochordates: Herdmania, Amphioxus (section through pharynx) Agnatha: Petromyzon and
Ammocoetus larva.
Fishes: Trygon, Acipenser, Protopterus, Echeneis and Hippocampus.
Accessory respiratory organs: Anabas.
Amphibia: Ichthyophis, Salamander and Alytes.
Reptiles: Chelonia, poisonous and non-ponsonous snakes (Krait, Cobra, Eryx, Typhlops).
Birds– one representative of ratitae and carinatae.
Mammals: Echidna, Platypus and Loris.
Microbiology
1. Sterilization
2. Culture preparation
3. Culture of microbes
4. Gram staining (+ve and -ve) (Demonstration)
B.Sc. (vi) - SEMESTER II – PART III c : ALLIED – I

ANCILLARY ZOOLOGY II 4 Hours / week 3 Credits
Chordata and General Principles
UNIT I: Prochordates:
Basic chordate characters – Amphioxus – anatomy.
UNIT II: Pisces

General characters – outline classification – type study – Shark – parental care in fishes –
Common edible fishes of South India (about 10 fishes)
UNIT III: Amphibia

General characters – outline classification upto orders with few examples in each – Parental care
in Amphibia.
UNIT IV: Reptiles

General characters – classification up to orders – Poisonous reptiles – identification of poisonous
and non-poisonous snakes of India – Poison apparatus and its function, Reptiles of economic
importance.
UNIT V: Birds
General characters – fossil birds – migration in birds – economic importance.
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UNIT VI: Mammalia
General characters – classification upto 3 major groups – Aquatic mammals – flying mammals.
UNIT VII: Physiology

Blood – composition – functions – coagulation – groups.
UNIT VIII: Ecology

Environmental pollution – wildlife conservation.
UNIT IX: Embryology

Gametogenesis – fertilization – parthenogenesis – placentation in mammals.
Unit X: Genetics
Sex determination – Syndromes.
Reference Books:
1.Manual of Zoology – Ekkambaranathan Ayyar and Ananthakrishnan.
B.Sc. (vi) - SEMESTER III – PART III a – MAJOR

CELL BIOLOGY AND DEVELOPMENTAL BIOLOGY 3+3 Hours / week 5 Credits
Cell Biology
Objective: The Course is intended to enable the student to understand the structure and
consequently to appreciate the functioning of the cell and its components. A brief history of the
development and future of this field is included to enable the student to pursue higher studies in
Cell Biology.
Unit:I – Brief History of Cytology (6 classes)

Invention of the microscope -Discovery of the cell-Foundations of the cell theory and its
contributions to Biology.
Unit: II – Methods of cell study (12 classes)

Principles of the light microscope - Histochemical techniques of fixation
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Freeze-drying and autoradiography - Biochemical techniques of cell fractionation, Principles of
Electron microscopes.
Unit: III – Ultrastructure, Biochemistry and functional role of (16 classes)

Plasma membrane -Endoplasmic reticulum -Ribosomes - Golgi Complex – Lysosomes -
Mitochondria - Centrioles -Nuclear components, chromosomes, nucleic acids.
Unit: IV – Genetic code (12 classes)

Nature of Genetic code – characteristics of triplet code – cryptoanalysis of genetic code.
Unit :V- Protein Biosynthesis

Role of mRNA, tRNA – Activation - Initiation – Elongation – Termination process.
Unit:VI – Cell Division (10 classes)

Mechanics of Mitosis and Meiosis -Parthenogenesis.
Reference Books:
1. Cell Biology, Structure, Biochemistry and Function – Phillip Sheeler and Donald E.
Bianchi, 2nd Ed. John Wiley And Sons, 1983
2. Cell Biology – DeRobertis et al., VIIth Edition, W.B.Saunders Company, 1985
3. Cytology – P.S. Verma & V.K.Agarwal 8th Edition S.Chand & Company,2004
4. Cell and Molecular Biology- DeRobertis, 8th Ed., Lippincott,2005.
Developmental Biology
INTRODUCTION
Historical review – Ontogenic and Phylogenic development
UNIT I : GERM CELL

Structure of sperm and egg (Human) – Types of eggs. Egg membranes – egg organization – Role
of Pituitary and other related endocrines in gametogenesis – Estrous cycle.
UNIT II : FERTILIZATION
Mechanics - Physiology – Theories and development – Experimental works in Fertilization -
Biochemical changes during fertilization
UNIT III : CLEAVAGE AND GASTRULATION
11
General principles – Outlines of Physiology – Comparative account in Frog, Chick and Man
UNIT IV : EMBRYONIC ADAPTATIONS

Embryonic Membranes and their function – Placentation in mammals
UNIT V : PARTHEONOGENESIS AND REGENERATION

Artificial partheonogenesis – Experiments on Artificial Parthenogenesis and their findings –
Regeneration in invertebrates and vertebrates – Regeneration studies in Amphibians.
Types of Placenta in Mammals
UNIT VI : HUMAN DEVELOOPEMENT

Puberty and related changes – Menstrual cycle – Menopause – Pregnancy and related problems –
Parturition – Lactation – Contraception and family welfare.
UINT VII : REPRODUCTIVE TECHNOLOGY

Super ovulation – Synchronisation of estrous – Artificial insemination – Embryo collection –
Semen and Embryo preservation – Embryo transfer – Pregnancy diagnosis – Amniocentesis.
Reference Books:
Balinsky – Text Book of Embryology
Nelson – Comparative vertebrate embryology
Berril – Developmental Biology
Raven – Developmental Physiology
Barrington- Structure and Function of Invertebrates
Subramonium – Developmental Biology
Berril & Berth – Development
McEwven – Comparative Vertebrate Embryology
B.Sc. (vi) - SEMESTER III - PART III a: MAJOR

ENDOCRINOLOGY 4 Hours / week 5 Credits
UNIT I - Endocrine glands

Localization – structure – function and secretion.
UNIT II - Hormones
12
General function – types of hormones – steroids – peptides - second messenger – transport –
metabolism.
UNIT III
Receptors and hormones.- Mechanism of action
UNIT IV: Pituitary

Structure – synthesis – release and action
UNIT V Thyroid
Structure – secretion - functions – parathyroid and their role - Pathophysiology
UNIT VI: Adrenal

Structure – secretion – function - Pathophysiology
UNIT VII: Endocrine functions of the ovary and testis

Cyclic events of ovaries - Pathophysiology
UNIT VIII
Pineal, thymus and gastrointestinal hormones and their significance.
UNIT IX
Hormones in invertebrates.
Reference Books:
D. Turner, General Endocrinology.

Williams –Endocrinology
B.Sc. (vi) - SEMESTER III – PART IIIc: ALLIED II

BIOCHEMISTRY - I 4 Hours / week 3 Credits
13
UNIT I
Physicochemical forces acting on the living body:
Definition of pH, its determination, maintenance of pH in blood – water - buffers and electrolytes
in the body and their functions, Definition of osmosis (a method of determining osmotic pressure
- oedema and dehydration).
UNIT II
Carbohydrates: Classification and structure of monosaccharides (glucose and fructose in detail)
disaccharides (sucrose, maltose and lactose in detail), polysaccharides (starch, glycogen and
cellulose) - properties.
UNIT III
Proteins: Classification and structure of amino acid – properties. Classification and structure of
Protein – primary, secondary and tertiary structures - quarternary structure with Haemoglobin as
example – porphyrins.
UNIT IV
Lipids: General classification of lipids – saturated and unsaturated Fatty acids – names and
properties - characterization of fats, saponification value, iodine value - compound and derived
lipids.
UNIT V
Enzymes: Definition, classification, mechanism of enzyme action, enzyme substrate complex,
enzyme inhibitors, Factors affecting enzyme activity.
Reference Books:
Lehninger, Albert L. 1970. Biochemistry Worth Publishers, Inc., New York, pp.833.
Jain, L.L. Sunjay Jain & Nitin Jain 2005. Fundamental of biochemistry, S. Chand and
Company Ltd., New Delhi, pp.1230.
Ambika Shanmugam 1974. Fundamentals of Biochemistry for Medical Studies. Second

Edition (Revised), Aries Agencies, Chennai, pp.647.
Biochemistry – Satya Narayanan
14
B.Sc. (vi) - SEMESTER III and IV – PART III c : ALLIED II
BIOCHEMISTRY PRACTICAL 2+2 Hours / week 4 credit
1. Preparation of solution with ppm, ppt and percentage concentration.

2. Preparation of different molar solutions.
3. Preparation of different normal solution
4. Principles of titrimetry
5. Preparation of buffers with varying pH.
6. Qualitative analysis of Carbohydrates.
7. Qualitative analysis of Proteins.
8. Qualitative analysis of Lipids.
9. Quantitative estimation of carbohydrates.
10. Quantitative estimation of proteins.
11. Quantitative estimation of triglycerides.
12. Quantitative estimation of moisture
13. Separation of aminoacids by paper chromatography.
14. Colorimetric estimation of Creatinine.
15. Determination of enzyme activity : Salivary Amylase
16. Determination of enzyme activity: Alkaline Phosphatase
17. Principles of colorimetry and centrifugation.
15
B.Sc. (vi) - SEMESTER III – PART IV b : Inter Disciplinary
WILD LIFE MANAGEMENT 4 Hours / Week 3 Credits
Preamble: This paper deals with wild life from an Indian point of view. The objective of this paper
is to expose the student to the potential of Indian forestry and wildlife and to equip him/her to
undertake minor projects in this area. He/she will also be in a position to attempt questions in this
topic in competitive examinations.
UNIT I Introduction
Definitions, ancient history of wild life in India and its present status. Scope fo wild life biology.
Governmental and NGO involvement in protection and conservation of wildlife. Relationship
between animals and man.
UNIT II : Wildlife ecology

Aquatic, terrestrial, forest and mountain ecosystems. Food chain and food web. Animal
populations – natality, mortality, fecundity and growth. Intraspecific and interspecific
relationships. Dispersal and factors influencing dispersal. Population analysis and interpretations.
Effects of man’s involvement on wildlife. Scrub jungle ecology.
UNIT III: Wildlife in India.

Endangered species and economically important fishes. Amphibiology – endangered species and
economically important amphibians. Herpetology – endangered species and economically
important reptiles; special emphasis on Olive Ridley, Crocodiles and snakes. Ornithology –
endangered species and economically important birds; bird migration, nesting, bird watching,
birds in relation to humans. Mammalogy – endangered species and economically important
mammals, Mammals in human life.
UNIT IV¨ Wildlife management

Concepts and principles; planning and execution. Habitat evaluation – physical and remote
sensing. Habitat conservation and protection. Capture and marking technique for census and
treatment – bird rings and radio collars.
UNIT V: List & location of Zoos, Sanctuaries, Parks and Biosphere reserves of India.
Role of these in preservation and conservation management. Rehabilitation programmes (eg.
Project Tiger). National parks – Khaziranga, Gir, Bandhipur, Kanha, Guindy, Corbett, etc. Marine
National Parks – Mannar, Kutch, Point Kalimer, etc. Sanctuaries – Periyar, Mudumalai,
Vedanthangal, Bhandipur, etc. Zoos – Mysore, Trivandrum, Vandalur, Hyderabad, etc.
UNIT VI: Forestry and Sylviculture

Major vegetation types in India – classification, characteristics, composition and distribution.
Indian Forest Act (1927) – Forest Conservation Act (1985).
UNIT VII: Wildlife conservation and Legislation
16
Administrative set up – Central, State, Quasigovernmental. Statuary bodies (IBWL, BNHS, ZSI,
WWF, etc). Wildlife protection Act (!972), Rules, regulations and punishment, “Red Data Book”.
Reference Books:
1. The Development of International Principles and Practices of Wildlife Research
Management by Stephen H. Berwick and U. B. Sahania
2. Ecology of a changing planet by Mark B. Bush
3. Human import on ecosystem by Trivi and O’Hore
4. National Parks of Madhyapradesh by S. K. Tiwari
5. Endangered Animals of India and their Conservation by S. M. Nair
B.Sc. (vi) - SEMESTER IV- PART III a – MAJOR
MOLECULAR BIOLOGY AND GENETICS 3+3 Hours / week 5 Credits
Unit I
Nucleic acids: DNA-chemical structure-Purine and Pyrimidines – Chargaff ‘s rule.
Watson and Crick model. RNA: chemical structure. Types of RNA: mRNA, tRNA,rRNA
Differences between DNA and RNA.
Unit II
DNA synthesis: DNA replication; origin- fork direction; primer, leading and lagging strand,
enzymes.
Unit III
Transcription: andTranslation : RNA polymerase in prokaryote and eukaryote. Initiation –
Elongation- Termination. HnRNA.Post –Transcriptional modifications: Splicing: splceosome,
intron exon polyadenylation, capping. Reverse transcription –reverse transcriptase-cDNA.
Genetic code -Initiation, Elongation and Termination of Translation in prokaryotes and
eukaryotes.
Unit IV
Regulatory mechanism; Operon model- Jacob & Monod Lac Operon-positive and negative
regulation.
Unit V
Fundamentals of Genetics: Mendel’s Law, Monohybrid Inheritance- complete dominance,
incomplete dominance, codominance.. Dihybrid Inheritance- Independent assortment-Test Cross.
Interaction of alleles- complementary , Supplementary and Epistasis. Multiple alleles: Blood
Groups-ABO, MN and Rh Blood Groups, Coat colour in rabbit.
Unit VI
Linkage-Bateson’s and Punnett’s work on swet pea. Crossing Over- Types, cytological evidence
and significance. Mapping of chromosome.
Unit VII
Sex Determination: Various Theories;Types-XX-XY, XX-XO, ZZ-ZW. Sex linked inheritance-
colour blindness, haemophilia. Gynandromorphs; Human sex linked anomalies.
Cytoplasmic Inheritance: Kappa particles in Paramecium, maternal effect in Limnaea
Unit VIII
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Mutation: Types Spontaneous – Induced- mutagens-Tautomerizatiom, dimer formation and
methylation. Gene mutation –Types-Frameshift, position; addition , Deletion, and substitution.
Chromosomal abberration-Numerical: Euploidy, Aneuploidy and Polyploidy. Structural:
Inversion, Transversion, Duplication, Deletion.Homologous and Non-homologous Translocation.
UnitI X
Applied Genetics:
Eugenics, Euthenics and Genetic Engineering.
B.Sc. (vi) - SEMESTER IV – PART III a : MAJOR

CYTOGENETICS, DEVELOPMENTAL BIOLOGY + ENDOCRINOLOGY PRACTICAL
2+2 Hours / week 5 Credits
Cytogenetics
1. Microscopy - dissection and compound microscopes - identifying the different Components.

2. Dermatoglyphics - ulnar, radial loops, whorls and ridge count
3. Karyotypes - Normal male, normal female, Klinefelter, Turner and Down’s
syndromes.
4. Identification of prepared permanent slide of : Squamous and columnar epithelium, adipose, bone,
cartilage and muscle tissues.
5.Blood smear of man.
6. Blood grouping - A,B,AB,O & Rh+ve and Rh- ve in human beings.
7. Staining Buccal epithelium (Barr bodies)
8. Mitosis - Preparation, staining and study of different stages in onion root tip.
9. Meiosis - Preparation, staining and study of different stages in grasshopper testis.
10. Study of giant chromosomes in Chironomous larvae.
1. Morphometrics of sperm and ovum
2. Preparation of Sperm smear
3. Study of egg types, cleavage, blastula and gastrula stages of Amphioxus & Frog
4. Dissection of Tadpoles to observe structural peculiarities
5. Study of Human foetal stages
6. Study of developmental stages in chick: 24, 36, 48 and 72
7. Study of pig or goat uterus to observe the placentation type.
Endocrinology
1.Observation of histological studies of endocrine organs
a) ovary b) testis c) thyroid d) adrenal cortex & medula e) pancreas – ilets of langerhans
f) thymus e) pituitary
2. Estrous cycle - observation in vaginal smear of mice
3. hCG - based pregnancy test.

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B.Sc. (vi) - SEMESTER IV – PART III c – ALLIED II
BIOCHEMISTRY – II 4 Hours / week 3 Credits
UNIT VI
Metabolism of Carbohydrates – Glycolysis, Krebs cycle, HMP shunt, Glycogenesis,
Glycogenolysis, Gluconeogenesis
Metabolism of Proteins: oxidative deamination, transmination, urea cycle.
(Biosynthesis of proteins – basic pattern)
Lipids: Fatty acid breakdown – formation of ketone bodies (Biosynthesis of fats). Cholesterol
biosynthesis.
UNIT VII
Nucleic acids – structure & significance
UNIT VIII
Bioenergetics: I and II Law of Thermodynamics – Free energy, enthalpy of entropy, oxidative
phosphorylation, electron transport chain: ATP - Bioenergetics of Glucose fatty acid.
UNIT IX
Vitamins: Fat soluble vitamins (Vitamins A, D, E and K) water soluble vitamins (Vitamin B
complex and C) with reference to their occurrence and requirements for humans.
UNIT X
Hormones: Definition, names of various endocrine glands and their secretations – elementary
study of the biological functions of insulin, thyroxine and sex hormones, mechanisms of hormone
action.
Reference Books:
Jain, L.L. Sunjay Jain & Nitin Jain 2005. Fundamental of biochemistry, S. Chand and Company
Ltd., New Delhi, pp.1230.
Ambika Shanmugam 1974. Fundamentals of Biochemistry for Medical Studies. Second Edition
(Revised), Aries Agencies, Chennai, pp.647.
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B.Sc. (vi) - SEMESTER V – PART III a : MAJOR
BIOTECHNOLOGY 5 Hours / week 4 Credits
Unit I: Molecular organization of Cells

Organization of DNA into chromosomes, Histones and DNA – nucleosomal structure –RNA
synthesis and RNA processing, RNA processing to form mRNA coding for several proteins – role
of nucleolus.
Unit II: Gene Technology

Gene manipulation – basic techniques of cloning, vectors used in cloning, Restriction enzymes,
linkers, adapters – expression of cloned genes, PCR- Gene expressionstructures- products and
analysis.
Unit III: Enzyme technology

Traditional use of enzyme – immobilization of enzymes and applications.
Unit IV: Bioprocess technology

Exploitation of microorganism – fermentation technology. Immunotechnology – vaccine
production, molecular probes, hybridoma technology – monoclonals. bioremediation etc..
Unit V: Environmental biotechnology

Environmenmt and human health; biomonitoring of pollution- waste treatment
Unit VI: Applications.
20
Animal biotechnology: transgenic animals, high yielding and disease resistant animals;
Transferring genes into animal oocytes, eggs, embryos and specific animal tissues. Gene transfer
to plants.
Reference Books:
Molecualr biology of cell, Bruce Alberts, Weber, et al.
Molecular Biology of Gene, Watson.
Biotechnology, principles and practices, Primrose.
Concepts in biotechnology, Balakrishnan et al.
Fundamentals of Biotechnology, Gupta
B.Sc. (vi) - SEMESTER V – PART III a: MAJOR

BIOPHYSICS, EVOLUTION& ANIMAL BEHAVIOUR (2+2+2) Hours / week 4Credits
Biophysics
The subject is introduced to Zoology students to know the physical approach of biological system
and to have an overall idea about the subject content.
Unit-I: Introduction: Scope of Biophysics; Concept and division of Biophysics; Understanding

the physical problems of body; Basic physical laws and its definitions.
Unit-II: Energetics: Energy in different forms; laws of thermodynamics; Energy flow in

biological systems.
Unit-III: Molecular Biophysics: Structure of biomolecules – Protein, haemoglobin, myoglobin,

actin, tubulin.
Unit-IV: Methods of studying molecules: Principles of Centrifugation, Spectrophotometer,

NMR , X-ray diffraction.
Unit-V: Biomembrane: Structure of Phospholipids, hydrophobic effects, self assembly,

membrane potentials, flux across membranes, selectivity- Nernst, Na, K and ATPase, Donnan; Ion
composition-intracellular fluid and extracellular fluid.
Unit-VI: Electrophysiology: Nerve conduction and Muscle mechanics-contraction.
21
Unit-VII: Clinical Biophysics: Radiation-radioactive decay, hazards of radiation and
radiotherapy; Principles of Ultrasound and CT scan.
Reference:
1. A Text book of Biophysics, R.N. Roy, Central Publication 2005
2. Biophysics and Biophysical Chemistry, Debajyoti Das, Academic Press, 2001
3. Essentials of Biophysics, P.Narayanan, New Age International (P)Ltd., 2007.
Evolution
UNIT I
Origin of life, Growth of evolutionary concepts
UNIT II
Evidences in support of organic evolution.
Evidence from Comparative morphology and Anatomy, Embryology, Palaeontology, Physiology
and Biochemistry, Genetics, Taxonomy, Geographic distribution
UNIT III: Theories of Evolution

Abiogenesis – Biogenesis- Cosmozoic theory – Lamarkism – Darwinism – Neolamarkism, Neo
Darwinism – Modern synthetic theory of natural selection– Germplasm
UNIT IV: Some general principles

Attavism, Cope’s rule – Allen’s rule – Allometry – William’s rule – Orthogenesis- Extinction,
specialization.
UNIT V: Adaptation
Types –Mimicry – Coloration – Adaptive radiation in Darwin finches and mammals– Adaptive
convergence.
UNIT VI: Distribution of Animals and Speciation

Barriers – Isolation and related mechanisms.The species concept – types and process.
UNIT VII
Evolution of Man , Cultural evolution of man.
Reference Books:
Evolution – process and product, Dodson
Introduction Evolution, Moody.
Animal Behaviour
Unit I : Introduction
Definitions - ethology, instinct, learning, stimulus, drive and motivation, agonistic behaviour,
territorial behavior, courtship behavior. Specific Action Potential (SAP), Psycho-hydraulic model
of release of Fixed Action Pattern (FAP).
Unit II : Animal Orientation

Tropism, kinesis (definition and classification with examples), taxes (definition and classification
with examples). Migration (definition and description of migration in birds).
22
Unit III : Learning behaviour
Definitions and classification of learning behaviour with examples (habituation, instinct, classical
conditioning, trial and error learning, insight learning, imprinting).
Unit IV : Social Behaviour

Communication : Definition and significance of communication. Communication through vision
– bee dance, communication through sound - birds, bats (echolocation through ultrasound);
communication through chemicals and pheromones.
Courtship: Definition and description with suitable examples from the animal kingdom.
Agonistic behaviour : aggression and appeasement behaviour : definition and description with
suitable examples.
Territorial behaviour : Definition and description with suitable examples.
Unit V : Rhythmic Behaviour of Animals

Types of biological rhythms (definitions) – circadian, lunar, tidal, annual; terminologies used
(zeitgeber, phase, phase shift, entrainment, free running rhythm,. actogram, masking, arrhythmia).
Properties of circadian rhythms. The role of different hormones in the control of behavior, with
examples.
Unit VI : Human Behaviour

Actions: Inborn, discovered, absorbed, trained and mixed actions.
Gestures: Primary and secondary gestures , Baton signals, greeting signals.
Reference Books:
McFarland, D, Animal Behaviour
Manning, Introduction to Animal Behaviour
Desmond Morris, Manwatching.
B.Sc. (vi) - SEMESTER V- PART III a: MAJOR

BIOINFORMATICS & BIOSTATISTICS 5 Hours / week 4 Credits
Bioinformatics
Unit I: Introduction to Bioinformatics

Aims, Tasks and Applications of Bioinformatics. Information Retrieval System. Genomics –
Recombinant DNA technology - DNA sequencing. Applications of bioinformatics – Human
Genome Project, Sample software in biology.
Unit II: Biological Databases

Sequence, structure and literature databases. Data submission Tools, Data Analysis and Prediction
Tools. Nucleic acid and proteins sequence data banks. Databases of patterns, motifs and profiles:
Metabolic Pathway Databases. Specialized Software packages.
23
Unit III: Genomics and Proteomics
Genome sequencing technology, Whole genome analysis, Comparative genomics - Proteomics –
protein structure prediction (primary, secondary & tertiary) viewer for protein structure – PDB.
Gene to Protein Function: Analysis of Proteomes, Analysis of 2-D gels, Human Genome Project.
Reference Books:
1. Computers and Common Sense (Fourth Edition)- 1997
Roger Hunt, John Shelley, Prentice Hall of India Private Ltd.
2. Basic Bioinformatics 2005, S.Ignacimuthu, s.j.
Narosa Publishing House
3. Introduction to Bioinformatics ( Fourth Edition ) - 2003
T.K. Attwood & D.J. Parry- Smith, Pearson Education ( Singapore) Pvt. Ltd.
4. Introduction to Bioinformatics - 2003
Arthur M. Lesk , Oxford University Press
5. Bioinformatics- Methods and Applications- Genomics, Proteomics & Drug Discovery- 2005
S.C. Rastogi, N. Mendiratta & P. Rastogi , Prentice Hall of India Private Ltd.
6. Bioinformatics- Databases and Algoriths - 2006
N. Gautham - Narosa Publishing House
7. Discovering Genomics, Proteomics & Bioinformatics- 2003
A.Malcolm Campbell & Laurie J. Heyer, Pearson Education ( Singapore) Pvt. Ltd.
8. Bioinformatics- Managing Scientific Data- 2003
Zoe Lacroix & Terence Critchlow, Morgan Kaufmann Publishers.
Biostatistics
Unit I
Definition – history and development of biostatistics – importance of biostatistics, functions and
limitations of biostatistics, data in biology – samples and populations – definitions of variable,
variables in biology, accuracy and precision of data, derived variables – Indices, ratios,
percentages and rates.
Unit II
Collection of data: primary data – methods of collection, (survey experimental and observation
method), secondary data. Sampling and sampling design – law of statistical regulatory and law of
inertia of large numbers.
Types of samples – random and non-random samples.
Classification of data – based on differences in kind, quantitative, geographical, chronological and
alphabetical classification.
Tabulation of data – components of tables, types of tabulation – simple and complex. General and
special purpose tables.
24
Diagrammatic representation of data – differences between diagrams sand graphs, types of
diagrams – one, two and three-dimensional diagrams, pictograms, cartograms.
Graphical representation of data – classification of graphs – graphs of time series and frequency
distribution
Unit III
Measures of central tendency – arithmetic mean, other means (weighted, geometric and
harmonic), median, mode - measure of dispersion and variability – range, percentile, quartile
interquartile range or quartile deviation, mean deviation standard deviation, coefficient of
variation – Lorenz curve.
Reference Books:
Biostatistics – Jyothikumar
Statistical methods in bioinformatics - Ewens W J
Statistical methods in biology - Bailey Norman T J
B.Sc. (vi) - SEMESTER V- PART III a: MAJOR

BIOTECHNOLOGY & BIOINFORMATICS PRACTICALS
2+2 Hours / week 4 Credits
Biotechnology
1. Laboratory organization and GLP

2. Sterilization and Media preparation
3. Culture technique- Basic principles of animal cell culture
4. DNA isolation from bacteria ( Demo)
5. Genomic DNA isolation from animal tissues.
25
6. Separation of DNA by agarose gel electrophoresis
7. Estimation of DNA by Diphenylamine method.
8. Enzyme immobilization.
9. P.C.R.
10. Design of bioreactor.
Bioinformatics
1. Databases: Literature databases Pub Med, Medline.

2. Protein sequence database: Uni Prot
3. Nucleotide sequence database: Genbank.
4. Similarity search tools-Entrez, FASTA and BLAST.
5. EMBOSS – Pair Wise Sequence Analysis
6. CLUSTAL W – Multiple Sequence Analysis
7. DS Visualizer- Protein Visualization
8. RasMol
B.Sc. (vi) - SEMESTER: V and VI – PART III b. ELECTIVE

a. AGRICULTURAL ENTOMOLOGY 2+3 Hours/week 2+3 Credits
UNIT I: Introduction to Entomology. Types of damage to plants by insects – insect pests –

Causes of insects assuming pest status- Introduction to agronomy.
UNIT II: Insect pests of crops:
26
Pests of Rice: Rice leaf folder, Rice stem borer, Rice thrips, Rice gall midge, Rice mealy bug,
Green rice leaf hoppers, Brown plant hopper, Rice earhead bug, Rice grass hoppers, Whorl
maggot.
Pests of Sugarcane: Sugarcane Shoot borer, Stem or internode borer, Top borer, Termites, White
grubs, Sugarcane leaf hopper, Sugarcane scale, Mealy bug, Whiteflies.
Pests of Coconut: Rhinoceros beetle, Red palm weevil, Black-headed caterpillar, White grub.
Pests of Vegetables: Brinjal – Shoot and fruit borer, Stem borer, Spotted leaf beetle, Grey weevil,
Tomato – Fruit borers, Serpentine leaf miner, – Pests of lady’s finger and gourds.
Pests of Beverages: Coffee – Coffee white borer, Red borer, Green scale, Tea – Tea mosquito bug
– Pests of Cocoa.
Pests of Stored products: Rice weevil, Sweet potato weevil, Lesser grain borer, Tobacco beetle,
Drug store beetle, Pulse beetle, Tamarind beetle, Red flour beetle, Indian meal moth, Fig moth,
Rice moth, Khapra beetle.
UNIT III: Methods of pest control – Natural control – Applied or artificial control – Prophylactic
methods – Curative or direct methods – Cultural methods – Mechanical methods – Physical
methods – Biological methods – Chemical methods – Insect attractants – Repellents – Anti
feedants – Genetically modified crops – Bt cotton – Bt brinjal – Bt maize.
UNIT IV: Pesticides – Classification of Insecticides – Inorganic compounds – Organic

compounds – Synthetic organic compounds.
UNIT V: Plant protection appliances – Dusting and dusters – Spraying and sprayers – Aircraft
application of pesticides – Other equipments.
UNIT VI: Integrated Pest Management
REFFERENCE:
Elements of Economic Entomology – B. Vasantharaj David
B.Sc. (vi) - SEMESTER: V and VI– PART III b. ELECTIVE

a. SERIBIOTECHNOLOGY 2+3 Hours/week 2+3 Credits
UNIT 1 INTRODUCTION
27
Silk producing organisms-tasar silk-muga silkworm-eri silkworm-anaphe silkworm-gonometa
silkworm-fagara silkworm-coan silkworm-spiders-mussel-uses of silk-history of sericulture-silk
production in the world-sericulture in India-sericulture in pre-british period-sericulture during the
british period 10-sericulture after independence-the central silk board-members of CSB-functions
of CSB-organizational set-up of CSB-research institutes-CSR&TI,mysore and berhanpore-the
national silkworm seed project, Bangalore-the centralsilk technology research institute-the
silkworm seed technology laboratory [SSTL]-the seribiotech laboratory-training facilities in
sericulture-facilities available for sericulturists-national sericulture projects[NSP]-future scope.
UNIT 2 MORICULTURE
Geographic distribution- optimum conditions for growth- other uses -medicinal value
-classification
UNIT3 PACKAGE OF PRACTICES FOR MULBERRY CULTIVATION

Package of practices-planting material-Mulberry varietied recommended-preparation of land-
planting direction and season-planting distance-planting system-PIT system-row system-kolar
system-strip system-angular system- Irrigation- manuring- harvesting.
UNIT 4 DISEASES OF MULBERRY

Fungal, bacterial and viral diseases- -deficiency diseases.
UNIT 5 PESTS OF MULBERRY

Insect and non-insect pests
UNIT 6 BIOLOGY OF Bombyx mori

Taxonomic Position of Bombyx mori - life cycle of Bombyx mori - Races of Bombyx mori -
classification based on the number of larval moults – classification based on the voltinism-
classification based on place of origin- Indigenous pure races of India- Kashmir race- Borapalu-
C. Nichi- Chotopolu- Bulupulu- Nistari- pure Mysore- Nyapolu- Sarupat- Moria- Commercial
races of India- multivoltine Races- Bivoltine races- races evolved through mutation breeding-
races evolved through line breeding methods- races with sex-limited character.
UNIT 7 GRAINAGE TECHNOLOGY

Breeding stations- P4 and P1 stations- P2 stations- P1 stations- selection of A seed cocoon rearer-
grainages- procedures in a grainage- diapausing and non-diapausing egg- diapausing eggs-
storage of eggs- incubation constant temperature incubation- raised temperature incubation-
embryonic method of incubation- transport of eggs.
UNIT 8 REARING FACILITIES

Rearing house- bed cleaning- spinning larvae- disinfection-maintaining optimum conditions.
UNIT 9 REARING OPERATIONS

Disinfection-physical methods-chemical methods-brushing-brushing from loose eggs in egg
boxes-brushing from egg cards-maintenance of optimum conditions for rearing-temperature-
humidity-quantity of feeding-frequency of feeding-quality of leaf-preparation of leaf for feeding-
bed cleaning-frequency of bed cleaning-methods of bed cleaning-spacing-frequency of spacing-
methods of spacing-care during moulting-mounting-characterstic features of a ripe worm-process
28
of spinning-methods of mounting-population density in mounting-precautions to be taken during
mounting-harvesting.
UNIT 10 REARING METHODS

Chawki rearing of young age worms in India-paraffin paper rearing-box rearing-new net rearing-
co-operative rearing-chawki rearing methods in Japan-single feeding per instar-wet cloth rearing-
closed rearing-pack rearing-kasso rearing-showa rearing-co-operative rearing in Japan- rearing of
nature larvae-shelf rearing-floor rearing-shoot rearing.
UNIT 11 DISEASES OF BOMBYX MORI

Protozoan disease-bacterial disease [“flacherie”]-bacterial flacherie or gastric injury flachers-
septicemia-sotto disease-court disease-viral disease-infectious flacherie-gattine-grasserie-fungal
disease-disease of the adult moth.
UNIT 12 PESTS OF SILKWORM

Uzi fly-Indian uzi fly-Kuoso or Japanese uzi fly-black uzi fly-tasar uzi fly-dermestid beetles-the
straw mite (pedicolus ventricoses)-ants-namatode-lizards,rats,squirrels & birds.
UNIT 13 COCOON MARKETING

Transport of cocoon- physical characters of cocoons considered for commercial purposes-
defective cocoons- defects due to heritable racial characters- defects arising from mistakes in
rearing and mounting- defects due to parasitic infestation- defects due to moth emergence- cocoon
markets.
UNIT 14 SILK REARING

Stifling- sun-drying- steam- stifling- hot- stifling- storage of cocoons- sporting of cocoons-
deflossing- cocoon riddling- cocoon mixing or blending- cocoon cooking- boiling for top reeling-
boiling for sunken system of reeling- brushing- reeling operations- reeling end formation-
intertwining or twisting- drying and distribution of the thread on the reels- reeling appliances-
country charkha (traditional charkha)- domestic basin- cottage basin- filatures- re-reeling- lacing,
skeining- raw silk testing- visual tests- mechanical tests.
UNIT 15 BY-PRODUCTS OF SERICULTURE

By-products of moriculture-by-products of rearing-use as compost-use as animal feed-use as
suturing material-use as feed for biogas plant-use in pharmaceutical industry-by-products of
grainage operation-use of pierced cocoons-use of waste moths-by-products of reeling-defective
cocoon wastes-pupal waste-reeling wastes-commerical classification of silk thread wastes-filature
wastes-charkha wastes-spun silk.
UNIT 16 AN ASSESSMENT OF SERICULTURE IN INDIA AT PRESENT

Productivity in moriculture-productivity of silkworm races-bivoltine rearing-productivity of
reeling sector-prospects of sericulture.
29
B.Sc. (vi) - SEMESTER: V and VI – PART III b. ELECTIVE
b. BIOPROCESS ENGINEERING AND TECHNOLOGY 2+3 Hours/week 2 + 3 Credits
Bioprocess Engineering and Technology
UNIT I
Introduction to bioprocess engineering: Basic principles of bioprocess. Types of fermentation:
batch, fed batch and continuous fermentation systems. Microbes in industrial process: Isolation,
screening and maintenance of microbes for industrial processes. Strain improvement. Microbial
growth kinetics.
UNIT II
Upstream processing: Microbial Nutrition, Media formulation for industrial fermentation.
Development of inocula for the industrial fermentations. Scale up.
UNIT III
Bioreactors: batch, fed –batch and continuous bioreactors, biotransformation, stability of
microbial reactors, analysis of mixed microbial populations, specialized bioreactors (pulsed,
fluidized, photo bioreactors etc.
UNIT IV
Industrial production of chemicals, alcohol(ethanol),acids (citric, acetic and gluconic ),solvents
(glycerol,acetone,butanol,antibiotics(pencilliin,streptomycin,tetracycline),aminoacids (lysine,
glutamic acid),single cell protein. Enzyme and whole cell immobilization and their industrial
applications.
UNIT V
Downstream processing: Introduction, removal of microbial cells and solid matter, foam
separation, precipitation, filtration, centrifugation, cell disruptions, liquid – liquid extraction
chromatography, Membrane process. drying and crystallization. Effluent treatment D.O.C. and
C.O.D. treatment and disposal of effluents.
Reference Books:
1. Biochemical Engineering, Aiba,S., Humprey,A.E., and Millis,N.F., Unty. of Tokyo
Press, Tokyo.
2. Biochemical Reactors. Atkinson,B., Pion, Ltd., London.
3. Biochemical Engineering Fundamentals. Baily,J.E., and Ollis,D.F., McGraw – Hill
Book Co. New York.
4. Bioprocess Technology: Fundamentals and applications, KTH, Stockholm.
5. Process Engineering in Biotechnology, Jackson,A.T., Prentice Hall, Engelwood Cliffs.
6. Bioprocess engineering: Basic Concepts, Shuler,M.L., and Kargi,F., Prentice Hall,
Engelwood Cliffs.
7. Principles of Fermentation Technology. Stanbury,P.F., and Whitaker,A., Pergamann
Press, Oxford.
8. Bioreaction Engineering Principles , Neilson,J., and Villadsen,J. Plenum Press.
9. Chemical Engineering Problems in Biotechnology, Shuler, M.L.(Ed.), AICHE.
30
10. Biochemical Engineering, Lee,J.M., Prentice hall Inc.
11. Biochemical Engineering – Kinetics, Mass Transport, Reactors and Gene Expression,
Vieth, W.F., John Wiley and Sons, Inc.
B.Sc. (vi) - SEMESTER: V and VI – PART III b. Elective

b. VERMITECHNOLOGY 2+3 Hours/week 2 + 3 Credits
UNIT I
Concepts of vermitechnology, objectives, scope and application of vermitechnology. Endemic and
exotic species of earthworms. Distribution and seasonal dynamics of earthworms. Drilospheres
and vermicasts.
UNIT II
Special features of Lumbricidae, Megascolidae and Eudrilidae with suitable examples. Biology
and anatomy of earthworms : metamerism, musculature, locomotion, digestive system, nutrition,
reproductive system, clitellum and cocoon formation. Classification of earthworms.
UNIT III
Ideal conditions for vermicomposting and maintaining a vermiculture unit. Types of
vermicomposting - large scale and small scale vermicomposting. Vermicomposting - raised bed
method and pot method.
UNIT IV
Earthworms as bioreactors. Materials required for vermibed preparation. Bioremediation using
earthworms - minimizing pollution hazards and application of vermitechnology by recycling of
organic wastes.
UNIT V
Diseases caused in earthworms, attack of predators and parasites and suitable remedies. Solutions
for problems affecting worm bins. Properties of vermicompost. Collection of vermiwash and
methods to obtain vermiwash.
UNIT VI
Economics of vermiculture - cost benefit analysis : establishment of vermiculture unit,
infrastructure, raw materials, labour, accessories. Harvesting of vermicompost, packaging and
marketing.
Reference books :
1. Invertebrate Zoology - E.L.Jordan P.S.Verma
2. Principles and Practice of Soil Science - Naren Kumar, Dutta
3. Soil Microbiology - N.S.Subba Rao
4. Vermicomposting - P.K.Gupta
5. Vermiculture and Organic Farming - T.V.Sathe
31
B.Sc. (vi) - SEMESTER: V – PART IV b. SKILL BASED
VERMICULTURE 4 Hours/week 2 Credits
UNIT I :
Introduction: Definitions and concept of vermiculture. Soil:major types (red soil, black soil,
alluvial soil). Influence of soil organisms in vermitechnology - bacteria, earthworms,
entomofauna, mites etc. Litter degradation and decomposition.
UNIT II :
Types of earthworms : Endemic and exotic species of earthworms. Ecological classification of
earthworms - epigeic, anecic and endogeic forms. Physical, chemical and biological changes
caused by earthworms in soil - drilospheres and vermicasts.
UNIT III :
Vermicomposting - vermicomposting materials, vermicomposting methods (raised bed method
and pot method). Vermicompost - quality, properties and advantages over chemical fertilizers.
Problems in vermiculture and remedial solutions.
UNIT IV :
Natural enemies of earthworms - Pests, parasites and pathogens affecting earthworms. Uses of
earthworms in food and medicine - ayurvedic and unani medicine. Recycling of food wastes in
vermitechnology. Application and scope of vermitechnology.
UNIT V :
Establishment of vermiculture unit: materials required, conditions for maintenance of
vermiculture unit, harvesting of vermicompost, packaging and marketing - cost benefit analysis -
man power, infrastructure and other raw materials.
Reference books :
1. Invertebrate Zoology - E.L.Jordan P.S.Verma
2. Principles and Practice of Soil Science - Naren Kumar, Dutta
3. Soil Microbiology - N.S.Subba Rao
4. Vermicomposting - P.K.Gupta
5. Vermiculture and Organic Farming - T.V.Sathe
32
B.Sc. (vi) - SEMESTER: V – PART IV b. SKILL- BASED
COMPUTER BASICS 2 Hours/week 3 Credits
Unit I
Introduction : Components of computer – hardware and software, power supply, peripherals.
Installation of operating system
Unit II
MS word, MS excel, MS power point– short cut keys, saving documents, printing, scanning.
UNIT III
Internet : LAN, MAN, WAN, types of topology, requirements for internet connections, Integrated
Service Digital Networking and its utility, Internet Service Provider (ISP) – IP address, File
Tranfer Protocol (FTP).
Unit IV
Internet access : web pages, web browsers, search engines : google, yahoo, Uniform Resource
Locator (URL), electronic mail, installation of antivirus.
Reference books :
1. Computers and Common Sense (Fourth Edition), 1997 - Roger Hunt, John Shelley,
Prentice Hall of India, Private Ltd.
2. Essentials of Computer Applications - S.Mythili.S.Gopalan
33
B.Sc. (vi) - SEMESTER VI – PART III a : MAJOR
ANIMAL PHYSIOLOGY AND IMMUNOLOGY 4+3 Hours / week 5 Credits
Animal Physiology
UNIT I: Respiration
Availability of oxygen. Respiratory organs in animals. Properties and function of respiratory
pigments. Regulation of respiration
UNIT II: Circulation

Types of hearts. Composition and functions of blood. Cardiac rhythm, cardiac output, ECG,
Blood pressure. Electrical activity and properties of the heart. Regulation of the cardiovascular
function
UNIT III: Coordination (Neuromuscular and neuroendocrine)

Nerve impulse conduction. Ultra structure of muscle. Theories of muscle contraction
UNIT IV: Excretion

Structure and functions of different excretory organs in animals. Mechanism of urine formation
in man
UNIT V: Homeostasis
Significance. Mechanism of osmo-ion regulation in fresh water, estuarine and marine fishes
Reference Books:
Invertebrates Structure and
Immunology
Unit I Immunity- Definition. Types of immunity-Active and Passive.
Unit II The basis of immunity: Innate Immunity- Natural Barriers, Phagocytosis, Inflammation,
Complement system, lectin. Adaptive Immunity- Primary and secondary response, memory,
Booster response.
Unit III Antibody: Immunoglobulin; Basic structure, classes and role in immunity. Cellular
Immunity Hemopoietic Tissue: Types and functions of B and T cells; cytokines and Interleukines.
34
Antigen: Types and structure. Hapten; Immunogen. Antigen-antibody reaction:
Haemagglutination, Precipitation, Immunodiffusion -ELISA
Unit-IV Lymphoid organs: Primary and secondary organs- location, structure and role.
Bone marrow, Thymus, Bursa of Fabricius, Lymph node, Spleen, MALT, GALT.. Maturation of
Immuno-competent cells.
Unit V Clinical Immunology. Immune response of Infections caused by bacteria and virus.
Diseases- Immunodeficiency, Hypersensitivity and autoimmune. Transplantation-Types of Graft-
MHC-HLA.
B.Sc. (vi) - SEMESTER VI – PART III a : MAJOR

ENVIRONMENTAL BIOLOGY AND BIODIVERSITY 4+3 Hours / week 4 Credits
Environmental Biology
I. - Abiotic and Biotic factors: Ecosystem concept – Pond as an Ecosystem – Energy flow –
Ecological pyramids – Food chain – Significance of food-web. Biogeochemical cycles –
Nitrogen – Phosphorous and Carbon cycles.
II. Ecological factors: Water, pH, salinity, temperature and light as factors. Atmospheric
pressure – Moisture in the atmosphere – Hydrological cycle – Humidity.
III. Population Ecology: Basic concepts of population ecology – Characteristics of population –

Inter and intra specific relations among populations.
IV. Community: Characteristics of a community, Ecological succession – causes of succession,

Basic types of succession – General process of succession – Hydrosphere or hydarch;
Lithosphere – a xerosphere on rock; climax concept in succession.
V. Freshwater Habitat: Lentic community; Lake zonation, Thermal stratification of a lake;

Eutrophication; Lotic community-streams & rivers; Stream zonation and communities.
VI. Marine Habitat: Characteristics of ocean, Structure of ocean; Ocean zones; Biotic
communities of oceanic zone; Pelagic life of marine organisms and their adaptations –
Plankton as a community – Benthic life and adaptations – Intertidal; rocky, sandy and muddy
shores. Marine pollution; control of marine pollution.
VII.Terrestrial Habitat: Biomes – Latitudinal and Altitudinal life zones; Major Biomes; Desert,
grassland, Tropical rain forests, Temperate deciduous forests, Taiga and Tundra.
VIII.Environmental Pollution & Public Health:

Air pollution – sources of pollution, Acid rains; Ozone; Prevention and control of air
pollution. Water pollution; Sources of Pollution and Pollutants; Prevention and control of
water pollution. Land pollution: Solid waste Pollution; Radioactive pollution; noise pollution;
pollution control through law.
35
IX. Natural Resource and their Conservation:
Resources – types of resources; soils of India; Climate of India; climatic regions of India;
vegetation of India; Diverse fauna of India; Wild life of India; Endangered flora and fauna of
India, Natural parks and sanctuaries. Projects for endangered species, Biosphere reserves.
Conservation: soil, land, water conservation; Forest resources; Forest cover; Deforestation,
Afforestation.
Reference Books:
Fundamental of Ecology – Eugene P. Odum
Elements of Ecology – Clarke
Animal Population – Browning
Concepts of Ecology – Kormandy
Ecology & Environmental Science – H.R.Singh & Neeraj Kumar
Environmental Biology – P.D.Sharma
Biodiversity
Unit I Introduction
Defining biodiversity, convention on biological diversity, its role, goals, functioning and
perspectives.
Mega diverse nations protected areas and biosphere reserves in India, IUCN, categories of threats
– Marine Park.
Unit II: Biodiversity from taxonomic and evolutionary perspective:

Origin of scientific taxonomy, basics of taxonomic characterization, characterizing, species (the
morphological species concept, biological species concept, the phylogenetic species concept).
Taxonomic measures of species diversity, modern developments (database and expert
identification systems).
Unit III: Types of Diversity

Ecosystem diversity, (variety of habitats), species diversity, genetic diversity. Methods of
measuring species diversity, molecular methods of assessing levels of genetic diversity.
Unit IV: Human society and biodiversity, Cultural diversity, values of diversity.
Unit V: Legislation
A review of national and international treaties, conventions and laws, biodiversity and
international environmental law, intellectual property rights over biological products.
Unit VI:
Biodiversity conservations and sustainable use.
Reference Books:
Ferguson. C. 1980. Biochemical Systematics and Evolution.
36
Mayor. E. 1994. Principles of Systematic Zoology.
Agarwal. K.C. 2000. Biodiversity.
A.B.Choudhari and D.D.Sarkar 2002. Biodiversity Endangered.
Kumar.V. 2003. Biodiversity Principles and Conservation.
Kotwal P.C. 2003. Biodiversity Conservation in Managed Forests and Protected Area.
Trivedi. P.C. 2004. Biodiversity Assessment and Conservation.
India 2006: Ministry of Information and Broadcasting, Government of India, New Delhi.
B.Sc. (vi) - SEMESTER VI – PART III a: MAJOR

ANIMAL PHYSIOLOGY& IMMUNOLOGY AND ENVIRONMENTAL BIOLOGY &
BIODIVERSITY PRACTICALS 3+3 Hours / week 4 Credits
Animal Physiology and Immunology
1. Estimation of oxygen consumption in an aquatic animal (Fish)
2. Blood smear: Total and differential count
3. Qualitative analysis of excretory products of animals
4. Estimation of salt loss or gain in an aquatic animal in heterosmotic media
5. Enzyme activity in relation to pH
6. Microscopic observation of sections of - Bone marrow, Thymus, Spleen and Lymph node
7. Locate primary and secondary lymphoid organ in any vertebrate (Demo)
8. Human blood smear: Observation of white blood corpuscles
9. Agglutination test: Blood groups
10. Visit to research laboratories
37
Environmental Biology & Biodiversity
1. Estimation of oxygen content in two water samples.
2. Estimation of salinity in two water samples.
3. Estimation of carbondioxide in two water samples.
4. Qualitative analysis of plankton from any one sample.
5. Study of soil organisms-Species richness, abundance, density, frequency, species evenness.
6. Study of physical characteristics (Temperature, colour & texture) of the soil.
7. Identification of fauna of rocky shore.
8. Identification of fauna of sandy shore.
9. Identification of fauna of coral reef.
10. Study of termitarium.
B.Sc. (vi) - SEMESTER: VI – PART III b. Electives

c. ON SITE TRAINING
a) Agricultural Entomology 2 Hours/week 3 Credits
1. Land prepation for Paddy and vegetables

2. Survey of Pest in paddy and vegetable fields.
3. Estimation of damage
4. Pest control operations using sprayer and dusters
5. Harvesting
6. Rearing of biocontrol agents and field applications
38
Sc. (vi) - SEMESTER: VI – PART III b. Electives
a) Seri-biotechnology 2 Hours/week 3 Credits
1. Mulberry :- Land preparation – Preparation of cutting and planting – Irrigation –

Application of manures – Leaf harvest and preservation – Annual pruning
2. Sericulture:- Brushing – rearing - feeding – bed cleaning – bed spacing – harvesting –
marketing.

b)Bioprocess Engineering & Technology 2 Hours/week 3 Credits
1. Fermentation Process
2. Production of alcohol
3. Production of any antibiotics
4. Production of aminoacids

b) Vermitechnology 2 Hours/week 3 Credits
1. Establishment of a vermiculture unit - an overview - preparation of vermibeds.

2. Culture of earthworms - observation of their burrowing activity, formation of drilospheres and
vermicasts.
3. Analysis of soil organisms using Berlese funnel - beetles, millipedes, mites, dermapterans etc.
4. Study of soil termites - termite mounds, caste disintegration and social behaviour.
5. Study of natural enemies of earthworms - attack of vermieries by ants, scorpions, beetles,
mites, centipedes, snakes etc.
6. Study of litter degradation and decomposition of farmyard wastes - agricultural and animal
wastes.
7. Determination of soil pH.
8. Harvesting of vermicompost and packaging.
B.Sc. (vi) (Voc) - SEMESTER I – PART III b Electives - Vocational

FISH BIOLOGY - THEORY AND PRACTICAL 4+2 Hours / week 4 Credits
Theory
UNIT I
Introduction to taxonomy; Binomial nomenclature; Characters of Elasmobranches and teleosts;
Variations in scales, fins, mouth, teeth and other structures used in taxonomic studies
39
UNIT II
Commercially important orders, families, genera and species of Elasmobranches and teleosts;
Internal anatomy of fish – digestive system and associated organs, gills, swim bladder, accessory
respiratory organs, heart and circulatory systems, lateral sense organs
UNIT III
Identification of commercially important species of prawns, crabs, lobsters and cephalopods of
India – Organization of internal organs of prawn and cephalopod
UNIT IV
Length-weight relationship – Age and growth – Studies on stomach content and its significance –
Feeding in relation to age – Index of preponderance
UNIT V
Fish reproduction – Sexual dimorphism – Dichromatism – Hermaphroditism – Types of ovary –
Ovarian development – Stages of maturity – Types of spawning in Elasmobranches and teleosts –
Fecundity – Gonadosomatic index
Reference Books:
Jhingaran Y.G Fish and Fisheries of India.
Bal and Rao.Marine Fisheries of India.
Carl .E. Bond. Biology of Fishes.
Lagler .K.F, J.E Baradack and R.R.Miller.Icthyology.
Munro.Marine and Freshwater fishes of Ceylon.
FAO Fishes identification catalogue Indian Ocean.
Michael King. Fisheries Biology, Assesment and Management
Practicals
1. Study of external morphology of a teleost fish.
2. Study of the anatomy of a teleost fish.
3. Identification of important marine (Shark, ray, skate, mullet, milk fish, seabass, flat fish,
bream, goat fish and pompret) and freshwater (catla, rohu, mrighal, tilapia and mystis)
fishes.
4. Gut content analysis of fishes.
5. Study of food and feeding habit of a herbivorous fish.
6. Study of food and feeding habit of a carnivorous fish.
7. Study of food and feeding habit of an omnivorous fish.
8. Male and female reproductive organs of fish.
9. Maturity stages of ovary and testis – Gonadosomatic index (GSI).
10. Fecundity of fishes.
B.Sc. (vi) (Voc) - SEMESTER II – PART III b Electives - Vocational

CAPTURE FISHERIES - THEORY AND PRACTICAL 4+2 Hours / week 4
Credits
40
Theory
UNIT I
Capture fisheries in India; Freshwater fisheries – Status and resources of Indian major carps,
catfishes and freshwater prawn
UNIT II
Estuarine fisheries – Status and resources of finfishes – Mullet, Milkfish and Seabass; Status and
resources of shellfishes – Clam, Mussel and Oyster
UNIT III
Marine fisheries – Status and resources of pelagic fishery – Oil sardines, Mackeral, Tuna and
Seerfish; Status and resources of demersal fishery – Elasmobranchs, Flatfishes, Crustaceans
(shrimps, crabs and lobsters), Molluscs (bivalves, gastropods and cephalopods)
UNIT IV
Crafts and gears – Different types of nets and traps; traditional, non-mechanised and mechanised
gears
UNIT V
Fishery management – Principles and importance of conservation and management of fisheries,
Exploitation and management of EEZ, Role of Government organization – CMFRI, FSI, MPEDA
and TANUVAS
Reference Books:
Srinivastava C.R.L.Fishery Science and Indian Fisheries.
Jhingaran Y.G Fish and Fisheries of India.
B.N.Yadav.Fish and Fisheries.
S.P.Biswas Manual and Methods in Fish Biology.
Narasimhan, Molluscan Fisheries of India.
Santhanam.Fisheries Science
Practicals
1. Visit to Royapuram fish landing centre.

41
2. Visit to Pulicat fish landing centre.
3. Observation of crafts employed in Pulicat lake.
4. Observation of gears operated in Pulicat Lake.
5. Visit to Sathanur dam.
6. Observation of crafts and gears used in Sathanur.
7. By-catch studies – fish landing sites.
8. Analysis and interpretation of data.
9. Identification of prawns – Macrobrachium sp., Penaeus sp.; crabs – Portunus sps., Scylla
sp.; lobsters; clams; mussels; oysters; cephalopods – squid, cuttlefish, octopus.
10. Identification of fishes – oil sardine, mackerel, tuna, seer fish and flatfish.
B.Sc. (vi) (Voc) - SEMESTER III : Part III b. Electives - Vocational
42
AQUACULTURE – THEORY & PRACTICAL 4+2 Hours / week 4
Credits
Theory
UNIT I : Introduction: Scope and definition, Importance, Origins and growth of aquaculture,
Present status of aquaculture.
UNIT II: Selection of sites for aquaculture: Land based farms, Open water farms, Water quantity
and quality, Sources of pollution, Environmental impacts,
UNIT III: Pre-requisites of cultivable organisms (sea weeds, sponge, shell fish, prawn, shrimp
and crabs, echinoderm and fin fish), Exotic species, Common aquaculture species.
UNIT IV: Farm culture: Extensive, semi-intensive and intensive culture, integrated farming, cage
culture, pen culture, raft culture, raceway culture, Sewage fed fish culture. Monoculture and
Polyculture.
Unit V: Fresh water aquaculture: Present status, preparation of ponds, Liming and fertilization.
Nutrition and feeds: Feeding habits and food utilization, Energy sources, Brood stock and larval
nutrition, Live feed and artificial feed.
Pests , predators, diseases: Control of aquatic insects, and predatory and weed fishes, Methods of
weed control i.e., manual, chemical and biological, important fish and shell fish diseases.
Breeding and culture of freshwater prawns, polyculture with finfish, Air breathing fish culture,
and cold water fish culture.
UNIT VI: Coastal aquaculture: Introduction, Present status, Brackish water resources, Culture
practice in Bherries and Pokkali fields.
Important species of cultivable Penaeid Shrimps. Life history, hatchery production of seed and
transportation, Waste water treatment, Stocking, management and harvesting.
Breeding and culture of brackish water fin fish in relation to milk fish and Grey mullets
Mariculture: Culture of edible oysters, mussels, sea cucumbers, pearl oyster and sea weed
culture.
UNIT VII: Harvesting:

VII.1 : Harvesting methods- Harvesting drainable ponds, Seining undrainable ponds , harvesting
cage and raceway farm, harvesting mollusc.
UNIT VIII: Aquafarm management.
43
Practicals
a) Identification of cultivable organisms:
1) Fishes 2) shrimps 3) Prawns 4) Molluscs
b) Identification of aquatic insects, weeds and predatory weed fishes
c) Culture of live feed organism- Field training
d) Identification of parasites and diseases of fish/shrimp
e) Soil and water analysis
f) Study of few brands of Artificial feeds
g) Visits to:
1) Fish farm 2) Shrimp farm 3) CIBA 4) CMFRI
h) Training program.
44
BSc (vi) (Voc)- SEMESTER V- Part III b Electives- Vocational
FISH DISEASES AND POST HARVEST TECHNOLOGY-THEORY & PRACTICAL
4+2 Hours/Week 4 Credits
UNIT I: Fish, shrimp diseases and Fish health management–Infectious diseases-Bacterial, fungal,
viral, protozoan; Non infectious and nutritional diseases. Disease diagnosis, prevention and
control measures. Fish immunization and vaccines.
UNIT II. Fish as food commodity-Biochemical and nutritional value of fish; fish decomposition-
Rigor mortis, post rigor, rancidity, Autolysis. Fish Preservation and processing- Principles and
methods- Chilling, freezing, sun drying, salt curing, canning, smoking, irradiation.
UNIT III: Fishery products and by-products: Fish liver oil, fish body oil, fish flour sausage, ham,
Fish meal, fish silage, fish manure, fish glue, isinglass, fish leather, guano, Shagreen, dried fins,
fish roe, Squalene, and chitosan, ambergris, Sea weeds: By-products- Agar, Algin, and
Carageenan. Industrial and pharmaceutical uses.
UNIT IV: Sanitation in processing units and quality control of fishery products: Importance and
methods; HACCP ( hazard analysis and critical control point )principle and quarantine methods:
HACCP and Safety of canned foods and unreliability of post process sampling of canned foods to
ensure sterilization. Status of a batch of canned foods identifying CCPs and their monitoring by
specially trained personnel.
UNIT V: Fishery Economics: Definition and application of economic principles to fisheries.

Fishery resource as common property resource. Theory of production and law of diminishing
returns. Fish markets economics and valuations. “Fishery cooperatives” and their importance.
Planning and financing scheme for fisheries.Extension education- Objectives. Role of extension
in fishing villages development. Methodologies of extension.
Reference books:
1. Balachandran, K.K., Fish Canning Principles and Practices. CIFT, Cochin.
2. Gopakumar K., 2002. Text Book of Fish Processing Technology. ICAR, New Delhi .
3. Hall, G.M., 1992. Fish Processing Technology (Ed), Blackie Academic and Professional,
London.
4. Hersom, and Hulland, E.D,1980. Canned Foods.Chemical Publishing Company,Inc., New York
5. Larousse, J and Brown, B. E, 1997. Food Canning Technology. Willey VCH New York
6. Venugopal, V. 2006. Seafood Processing. Taylor & Francis Group, London.
Practicals:
1. Identification of common fish parasites
2. Field visits to study diagnosis and treatment of fin fish and shell fish diseases
3. Visit to fish processing plants
4. Filleting of fish, packaging and freezing.
5. Processing of Prawns, Lobster, Squid, Cuttle Fish, Crab etc. in different styles.
6. Identification of fresh and spoilt fish. Studies on physical, chemical and sensory changes.
7. Study of socio-economic status of fisher folk by field visits to fishing villages
45
B.Sc. (vi) (Voc) - SEMESTER V – Vocational
ENTREPRENEURSHIP DEVELOPMENT - THEORY
Hours / week Credits
UNIT I
Need, Scope and characteristics – Types of entrepreneurship – Importance of environmental

considerations- Municipal by law and insurance coverage.
UNIT II
Special scheme for technical entrepreneurs – Identification of opportunities –Market need, scope
and approaches for project formulation.
UNIT III
Criteria for principles of product selection and development – Institutions financing procedures
and financing procedures and financial incentives SIPCOT, SISI, TIDCO, TIIC.
UNIT IV
Creativity and innovation-Problem solving approach-Strength weakness opportunity and threat

(SWOT) techniques-Techno-Economic feasibility of the project-Licensing-Registration
procedures.
UNIT V
Critical path methods (CPM)-Project Evaluation Review Techniques (PERT) as planning tools for
establishing-SSI.
BOOKS RECOMMENDED:
Entrepreneurial Development – S.Saravanavel
Entrepreneurial Development – Gupta
46
B.Sc. (vi) (Voc) -SEMESTER VI – Part III b Electives - Vocational
AQUARIUM SCIENCE - THEORY & PRACTICAL 4+2 Hours / week 5
Credits
UNIT I: Ornamental fish biodiversity of India- History, live bearers and egg layers, common
ornamental fishes of India (Fresh water and marine); scope for entrepreneurs in ornamental fish
trade.
UNIT II: Construction and maintenance of aquarium: Material required, construction, Glass
cover, Hood, Aquarium stand, Installation, setting of Aquarium, Planting of aquarium, tank
conditioning and stocking, introduction of fish, Decoration, Aeration, Acclimatization,
Maintenance. Aquarium accessories: Air pumps, filters, functions of gravel bed, foam filters,
power filters, lighting, aquarium heaters.
UNIT III: Feed and feeding of ornamental fish: Nutritional requirements, types of fish feed,
available feeds in market.
UNIT IV: Sexual dimorphism in live bearers and egg layers; Live bearers- Sword tails, white sail
fin molly; red platy fish; guppy; Egglayers- Cichlids; Cyprinids; Characins (tetras); Anabantiods
(Gourami) loaches and catfish
UNIT V: Breeding techniques of aquarium fish: Sexing, selecting the parent; conditioning the fish
to breed; various types of breeding, larval rearing and transportation.
UNIT VI: Disease of ornamental fish and their cure: Bacterial, fungal, protozoan, crustacean,
viral
UNIT VII: Aquarium plants and their propagation: Types of aquarium plants; important aquarium
plants and their uses.
Reference books:
1. Archana Sinha & Radha C Das . 2004. Ornamental fishes. Dr SC Mukherjee, Director,
CIFE, Mumbai (Publisher). PP 54
2. M. Kanthaiyapparaju. Simple guide to Aquarium fish keeping. Kasthuri Publishers. PP
122.
3. Dick Mills. 2004. 101 Essential tips Aquarium fish. Dorling Kindersley Limited, Penguin
Group (UK). PP 72.
4. Gerhard Brunner. 1973. Aquarium plants. TFH. Publications, Inc, Ltd. PP 157.
Practicals:
1. Identification of important ornamental fish – Fresh water and marine
2. Hands on training in ornamental fish culture
47
3. Knowledge on identification of common pathogens of fish
4. Construction of a typical home aquarium
5. Collection of live feed organisms from the water bodies
B.Sc. (vi) (Voc) -SEMESTER VI – Part III b Electives - Vocational

FISH BIOTECHNOLOGY - THEORY & PRACTICAL 4+2 Hours / week 5
Credits
UNIT I. Fish Breeding: Synthetic hormones for induced breeding- GnRH analogue structure and
function.
UNIT II Transgenesis : Methods of gene transfer in fishes, single gene traits, screening for
transgenics, site of integration, applications, regulation of GMOs, IPR, Evaluation of GFP
transgenics, monosex production.
UNIT III Gene Bank and conservation; Cryopreservation of gametes and embryos.
UNIT IV Feed Technology: Micro encapsulated feeds, micro coated feeds, micro-particulate
feeds and bio-encapsulated feeds, mycotoxins and their effects on feeds.
Unit V: Genetic engineering and its application in fisheries: Recombinant DNA technology:
DNA modifying enzymes - types of restriction endonucleases (Type I, II and III), DNA/RNA
modifying enzymes (alkaline phosphatases, kinases, exonucleases, ligases, terminal transferases);
Vectors - plasmids (replication, copy number control and compatibility), phagemids, cosmids,
high capacity vectors (eg. BAC), Hosts: prokaryotic (selected E. coli strains) and eukaryotic
(selected yeast strains).
Unit VI- PCR – principle, types and applications; Structure and function of DNA polymerase
and reverse transcriptase. Genomic DNA library: construction, screening (PFGE-Pulse field gel
electrophoresis) and applications.
Reference Books:
1. Reddy. P.V.G.K; Ayyappan et al., (2005) “Text Book Of Fish Genetics and
Biotechnology”. 218p ICAR publications. ISBN:81- 7164 – 029- X.
2. Lakra.W.S et al.,( 2004) “Fisheries Biotechnology” Narendra Publishing house,
240p ,ISBN- 81 – 85375- 86- 0.
3. Sambrook J et al 1989. Molecular Cloning: A laboratory manual. New York: Cold
Spring Harbor, Vol 1-3.
4. T.A. Brown. 1998. Recombinant DNA. Academic Press, London.
5. T.A. Brown. (2002) Genomes - 2nd edition. John wiley & sons, Newyork. 572p.
6. Lewin Benjamin. GENES – IX, London; Jones & Bartlet publishers; 2008;
48
829P; ISBN: 10-0- 7637- 5222.
7. Primrose S.B, Twyman R.M (2006) Principles of Gene Manipulation and
Genomics (7th edn). Blackwell Publishing, Oxford UK
Biotechnology Practicals:
1 Field visits to TANUVAS, ornamental fish farms:
a) Induced breeding
b) PCR
c) Diagnosis of WSSV
d) Cryopreservation of gametes
e) Live feed culture
f) HACCP methods
M.Sc.
M.Sc. (vi) - SEMESTER I - PAPER 1 : MAJOR CORE
ANIMAL PHYLOGENY 6 Hours / week 4
Credits
Unit I : Principles of animal taxonomy. Terminology - systematics - nomenclature -taxonomy -

classification - identification - taxon - phenon - cladon and siblings - biological species concepts -
definition of species, genus, variety, race, strain and breed - Naming of species, sub-species,
genera, Zoological commission and Congress - International code of Zoological nomenclature.
Keys - definition and types. Atlas, check list, catalogue. Biosystematics - definitions - Classical
and Experimental taxonomy and Numerical taxonomy.
Unit II: Geological time scale
Unit III: Phylogenetic trees and their significance: Protozoan inter-relationships - Origin of
Metazoa – theories - Inter-relationships of Coelenterates and their symmetry.
Unit IV: Origin of Bilateria - Evolution of Turbellaria and their importance - Rhabdocoela as a
stem group.
Unit V : Origin and evolution of coelom.
Unit VI: Origin of metamerism and Annelidan inter-relationship.
Unit VII: Arthropod phylogeny.
Unit VIII: Molluscan evolution – Monoplacophora
Unit IX: Echinoderm larvae and evolutionary significance.
Unit X: Evolutionary trends among minor phyla (Rotifera, Acanthocephala, Pogonophora,

Sipunculida, Entoprocta, Ectoprocta, Brachiopoda and Chaetognatha.
Unit XI: Phylogenetic importance of Prochordates

49
Unit XII: Theories of origin and evolution of Chordates
Unit XIII: Fossil history of fishes (origin and early evolution) and of Amphibians, Reptiles,
(major groups) and of Birds and Mammals (origin and evolution of the three sub classes).
Reference Books:
The Invertebrates (Vol I to Vol ) – Hyman, L.H.
Arthropod Phylogeny – Gupta, A.P.
The Evolution of metazoan – Hadzi
Lower Metazoa: comparative Biology and phylogeny – Dougherty, E.C.
Evolution of Vertebrates – Colbert
Life of Vertebrates – Young
Vertebrate Paleontology – Romer.
M.Sc. (vi) - SEMESTER I - PAPER 2: MAJOR CORE
BIOPHYSICS 4 hours / week 2 Credits
Biophysics
Objectives and Scope: Physics to explain life system – physics affects life system – physics helps
life system.
Unit I Theoretical biophysics

Thermodynamics: Laws of thermodynamics, thermodynamics related to chemical reactions –
internal free and bond energy. Animal mechanics Motion – kinetics, kinematics, friction, stress,
strain and tarques.
Unit II Molecular Biophysics

Atoms and molecules, Biopolymer, polymerization of organic molecules.
Structure of molecules : Methods and principles of determining molecular weight. Physical
principles involved in centrifugation, Chromatography, Electron microscopy, spectrophotometry
and NMR – spectroscopy, Optical principles of Fluorescence, Polarizing, Phase contrast and
interference Microscope colloids sol/gel changes – emulsions – Tyndall effect.
Unit III Membrane biophysics

Structure and function of bio-membranes – ion channels – Gates – Pores and Pumps.
Unit IV Radiation Biophysics

Isotopes, Isobars, Radioactivity and Properties of α β and γ rays, detection of Radiation, Hazards
of Radiation, and tracer techniques.Non-Ionizing radiation, UV and infrared – definition and
properties of Laser.
Unit V: Physiological Biophysics

Biophysical aspects of vision and its Aberrations. Biophysical aspects of nerve impulse
transmission, Action potential. Biophysical aspects of muscle contraction. Biophysical aspects of
hearing.
50
Unit VI Clinical biophysics
Biopotential recording ECG, EEG and EMG
Imaging techniques. Photography, X-ray and Ultrasound.
Therapeutic applications Diathermy, Radiotherapy and Laser
Principles of CT scan and MRI scan.
Reference
Life Science Physics – Joseph W Kane and Morton M. Sternheim, John Wiley and Sons, New
York.
Biophysics – Caesey
Biophysical Science – Eugene Ackerman.
M.Sc. (vi) - SEMESTER I – Paper 4: Major Core
NANOSCIENCE 5 hours / week 3 Credits
Unit I: Basics of Nanoscience

History of Nanoscience - Definition of nanometer, Nanomaterials and Nanotechnology –
Scientific Revolutions – Types of Nanotechnology and Nanomachines – the Periodic table –
Atomic Structure - Molecular and atomic size – Surfaces and dimensional space – top down and
bottom up - Opportunity at the nanoscale.
Unit II: Quantum Mechanics

Limitation of classical physics - Introduction to quantum physics - Factors affecting to particle
size - Quantum confinement - Quantum dots - particle in a box, Quantum wires and wells
Unit-III: Properties and types of Nanomaterials

Mechanical, Thermal, Electrical, Magnetic and Structural. Size effect on shape, electronic
magnetic properties of nanomaterials - Carbon nanostructures- electrical and mechanical
properties - Carbon nanotubes (CNTs)- Single walled carbon nanotubes (SWNTs), Multiwalled
carbon nanotubes (MWNTs) - graphenes, fullerenes.
Unit IV: Bionanotechnology

Biology inspired concepts – biological networks - biological neurons - Quantum dot biolabeling -
fabrication of biosensors - DNA based biosensors – Protein based biosensors – Bionanostructures-
Micelles, vesicles, multilayer films, biological interactions - future potential.
Unit V: Nanotechnology in Health Care

Nanotechnology in pharmaceutical applications - micro and nano immunosensors and use of gold
and silver nanoparticles - DNA and antibodies, DNA Profiling - Prosthetic and Medical Implants
51
- Nanodiagnostics using nanorobots, in vivo imaging capability to detect tumors and genetic
defects and other diseases.
Reference Books:
1. Nanoscience and Nanotechnology – Fundamentals to Frontiers – Rao & Singh
Principles of nanoscience and Nanotechnology – Shah & Ahmed
M.Sc. (vi) - SEMESTER I – PAPER 3 : MAJOR CORE

MOLECULAR BIOLOGY AND MOLECULAR GENETICS 3+4 Hours / week 4
Credits
Molecular Biology
Objective : To make the students understand the molecular basis of genes
Unit I Nucleic Acids (DNA and RNA)

Structures of DNA – (Willkins’s and Watson’s Model ) – Types of DNA, Complimentary bases in
DNA, Properties of DNA, denaturation and renaturation kinetics. Types of RNA – structure of
tRNA, DNA – RNA differences.
Unit II Synthesis of DNA

Origin of replication – DNA polymerases – 5’-3’ replication – proteins involved in replication –
the end replication problem – telomerase – proof reading – methylation of DNA – brief
comparison of eukaryotic and prokaryotic replication.
Unit III DNA repair

Fidelity of DNA sequences – different types of DNA repair systems with special reference to SOS
response.
Unit IV DNA Recombination

Homologous genetic recombination – enzymes involved in recombination – Holliday model – site
specific recombination – prerequisites for recombination – transposons.
Unit V Transcription and Translation

52
RNA polymerase in Eukaryotes – initiation and termination of transcription, transcription factors
– splicing of RNA – Eukaryotic mRNA processing – transport of RNA from nucleus to cytoplasm
– tRNA and rRNA synthesis – transcription inhibitors, translation.
Unit VI Cell Proliferation

Significance of cell cycle in cancer – abnormal cell growth (Neoplastic growth) concept of
oncogenes and anti-oncogenes.
Reference Books:
Molecular Biology of the Gene Watson et al.
Biochemistry, Voet, D and Voet, J. John Wiley and Sons, Inc. 1995.
Text of Biochemistry Lehninger et al, CBS publishers and distributors, 1993.
Molecular Genetics
Unit I: Genetic code
Characteristics of the code - Triplet nature – degeneracy – universality – decoding of DNA -
tRNAs translation into peptides, polypeptides, initiation, lengthening, molecular basis of
degeneracy of DNA into RNA (copies).
Unit II: Genetic control of hormone regulation

Analysis of gene control, structure of a gene encoding a polypeptide hormone, housekeeping
genes, levels of gene control, Examples of housekeeping genes and homeotic gene.
Unit III: Gene expression and regulation: Operon concept. Constitutive versus inductive control.
Positive and negative regulation of gene expression.
Examples: Lac, tryptophan
Unit III: Mutation

Classification of mutations, types of chromosomal, gene (transition, transversion, addition,
deletion), tautomerisation, deamination and base analogues, alkylating and other mutagenic
agents, molecular effects of mutation, mutagen and antimutagens, mutator and antimutator
polymerases.
Unit IV: Microbial genetics

Molecular model for transformation, recombination maps and transformation, generalized genetic
transduction, bacterial conjugation, sex factor, F-episome, conjugation and mapping, F’-episomes
plasmid.
Unit V: r DNA technology

53
Types of vectors, plasmids, cosmids, YAC’s, bacteriophages etc. Restriction enzymes -
classification, Cloning, expression of cloned genes, gene therapy.
Unit VI: Ethics

Ethical issues that have arisen due to cloning, ART
Reference Books:
1.Molecular Biology of the Gene. (Fifth edition)

James D Watson, Tania A. Baker, Stephen P.Bell.Alexander Gana, Michael Levini, Richard
Lorick 2006
M.Sc. (vi) –- SEMESTER I - PAPER 4: MAJOR CORE PRACTICAL – I

MOLECULAR BIOLOGY AND MOLECULAR GENETICS
2+2 hours / week 2 Credits
1. Basic techniques identification of DNA by feulgen reaction

2. Identification of RNA by Methyl green and pyromine staining.
3. Identification of proteins by MBPB.
4. DNA extraction.
5. PCR (Demonstration)
6. Squashes and smears of chromatin and chromosomes. Sex chromatin – human buccal
smear.
7. Orange G and Aniline blue staining for heterochromatin.
8. Study of mitotic chromosomes using onion root tip and bone marrow aspirate.
9. Mitotic indices in Onion root tip and Grasshopper testis.
10. Meiotic indices in Grasshopper testis.
11. Drosophila Genetics, Preparation of culture medium and culture of Drosophila handling
and maintenance – study of the life history – mutant forms. Chromatographic studies of
eye pigments of various eye mutants.
12. Population genetics Location of tasters and non-tasters by PTC tests in a random sample.
13. Multiple factor inheritance studies in a random sample of student population with
reference to their height.
14. Case studies – family histories and pedigree charts.
54
15. Dermatoglyphics
16. Karyotyping.
17. Study of various syndromes with the help of metaphasic plate.
M.Sc. (vi) - SEMESTER II – PAPER 5 : MAJOR CORE

BIOINFORMATICS & BIOSTATISTICS 5 Hours /week 4
credits
Bioinformatics
Unit I Introduction to Bioinformatics

Aims, Tasks and Applications of Bioinformatics. Information Retrieval System, Data
submission Tools, Data Analysis and Prediction Tools.
Unit II Biological Databases

Nucleic acid sequence data banks. Databases of patterns, motifs and profiles: Metabolic
Pathway Databases. Literature Databases. Database Similarity Searches: BLAST, FASTA,
PSI-BLAST algorithms; Pair wise sequence alignment - NEEDLEMAN and Wunsch,
Smith Waterman algorithms; Multiple sequence alignments - CLUSTAL, PRAS; Patterns,
motifs and Profiles in sequences.
Unit II. Genomics.

Central Dogma hypothesis. Gene structure. Recombinant DNA technology. Gel
Electrophoresis, DNA Sequencing. Single Nucleotide Polymorphism. Genome analysis,
Genome mapping, Comparative genomics - Paralogs and orthologs, Phylogenetic
profiling. Genome Projects.
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Unit III Proteomics
Introduction to basic Proteomics technology, Protein structure and Functions- amino
acids and peptides; primary, secondary, tertiary and quaternary structures; protein
identification and characterization; protein structure and function prediction; Protein
Sequencing. Protein prediction from DNA sequence.
Unit IV Homology, Phylogeny and Evolutionary Trees

Sequence Analysis. Alignment and Phylogenetic Trees. Homology and similarity,
Phylogeny and relationships, Approaches used in Phylogenetic analysis, Molecular
approaches to Phylogeny, Phylogenetic Analysis databases, Hidden Markov Models for
homology modeling.
Unit V. Microarray Technology

Introduction to basic microarray technology, - concept of gene expression.
Unit VI. Drug Discovery and Pharmacoinformatics

Overview of the Drug Discovery process and the role of computational methods. Review
of basic biological concepts, Characteristics of a drug compound, Elements of molecular
pharmacology. Concept of receptor and target. Receptor binding and activation. Ligand-
receptor interaction, non-covalent bonds. Ligand into the binding site. Concepts of
pharmacophore, bioactive conformation and Active Analog Approach. Molecular docking
Reference Books:
3. Introduction to Bioinformatics 2003
A. Malcolm Campbell & Laurie J. Heyer, Pearson Education ( Singapore) Pvt. Ltd.
Biostatistics
Unit I
Collection and classification of data – Representation of data –Tabulation of data –Diagrammatic
representation of data – differences between diagrams and graphs. Graphic representation of data
–frequency distribution.
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Unit II
Elements of probability – sample space, sample point, sample event and venn diagram.Theoretical
distributions – fundamentals of Binomial, Poisson and Normal distribution – Central Limit
Theorem. Student’s t-distribution – characteristics. Use of t-test for small samples. Chi-square
test.
Unit III
Correlation – characteristics. Types, methods of study of correlation – scatter diagram method,
graphic method, Karl Pearson’s coefficient of correlation – rank correlation – concurrent deviation
method – significance. Regression – characteristics, dependent and independent variables – slope
and intercept – method of least squares.
Unit IV
Analysis of variance (ANOVA) characteristics – F-distribution, types and concepts of ANOVA –
simple classification (one-way) of ANOVA and Two way ANOVA.
Unit V
Computer Applications in Biology.
Reference Books:
R.C. Campbell, 1973, Statistics for Biologists
N.T.J. Bailey, 1985, Statistical Methods in Biology
W.C. Shester, 1982, Statistics for biological Sciences
S.N. Schneider 1980, Statistical Methods in biology
Lewis, A.W., 1974, Biostatistics.
M.Sc. (vi) - SEMESTER II - PAPER 6 : MAJOR CORE
GENERAL ENTOMOLOGY AND APPLIED ENTOMOLOGY
6Hours / week 4 Credits
General Entomology
Unit I Reason for insects as a successful group
Unit II: Insect classification

Outline classification of insects with examples.
Unit III: Basic knowledge of the following orders of insects with special reference to Indian
examples: Apterygota: Thysaneura, Protura, Collembola, Dipleura. Pterigota:
Exopterygota – Ephemeroptera, Odonata, Orthoptera, Thysaoptera, Mallophega, Anopleura,
Hemiptera. Endopterygota: Lepidoptera, Coleoptera, Diptera, Hymenoptera.
Unit IV : Morphology of head Sulci-tentorium – types of mouth parts – type of antenna

Morphology of wing hearing segment – wing venation of generalized insect region of wings –
wing coupling mechanism – legs and external genitalia types of larvae and pupae.
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Unit V: Morphology of internal organs: Elementary histology of alimentary canal - respiratory
structure - circulatory organs - Brain and sub-oesophageal ganglia. - Internal organs of
reproduction.
Unit VI: Structure and composition of integument – moulting

Metamorphosis and role of hormones in metamorphosis
Insect digestion – digestion of carbohydrates, fats, proteins – role of micro organisms in digestion
Elimination of nitrogenous wastes
Mechanism of respiration
Composition of Haemolymph and its function – Course of circulation - heartbeat
Reference Books:
Chapman, R.F. The Insects structure and function
Nayar, K.K. and David, General and applied entomology
Wigglesworth, V.B. The principles of insect physiology
B. Vasanthraj David, T. Elements of economic entomology
Snodgrass, G.E. Insect morphology
Snodgrass, Principles of insect morphology.
Richard O.W. and Davies, R.G. Imms’ General Text books of Entomology, Vol. I
Richard O.W., and Davies, R.G. General Text Book of Entomology, Vol. II
Mani, M.S. General Entomology.
Applied Entomology
Unit I: Economic Importance of Insects: Insect pests – types – causes for insects attaining pest
status – Types of damages to plants
Unit II: Agricultural Entomology: Biology, Nature, extent of damage and control of pests of
Paddy, Brinjal, Mango, Cotton
Unit III: Storage of Entomology: Nature of damage and control of external and internal feeders
of stored product
Unit IV: Veterinary Entomology: Nature of damage and control of insect pests of cattle, fowl,
sheep and goat
Unit V: Medical Entomology: Nature of damage and control of insects associated with human
beings – mosquito, house fly, fleas and head louse
Unit VI: Basic knowledge of forensic entomology
Unit VII: Beneficial insects: Kinds of honey bees – bee keeping – care and maintenance of an
apiary- Types of silk worms and silk – mulberry varieties – Biology and behaviour of lac insect
and lac cultivation
Unit VIII: Pest control: Methods and principles of insect pest control – Natural control
( Climate, topographic features and natural enemies) – Artificial / Applied control (prophylactic,
curative measures, cultural, mechanical, physical, biological, legal and chemical methods) –
recent trends in pest control- IPM
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Unit IX: Insecticides: Classification based on mode of entry, mode of action, chemical nature,
hazards of insecticides, resurgence and outbreak, plant protection appliances.
Unit X: Integrated pest management programme, plant quarantine and pesticides registration in
India.
Reference Books:
Chapman, R.F. The Insects structure and function
Nayar, K.K. and David, General and applied entomology
Wigglesworth, V.B. The principles of insect physiology
B. Vasanthraj David, T. Elements of economic entomology
Snodgrass, G.E. Insect morphology
Snodgrass, Principles of insect morphology.
Richard O.W. and Davies, R.G. Imms’ General Text books of Entomology, Vol. I
Richard O.W., and Davies, R.G. General Text Book of Entomology, Vol. II
Mani, M.S. General Entomology.
M.Sc. (vi) - SEMESTER II : PAPER 7 : MAJOR CORE
RESEARCH METHODLOGY 4 Hours /week 4 credits

Unit I – Introduction to Research Methodology

Research: Meaning, Objectives, types and significance – Research process.
Research problem: Selection, selection criteria, necessity techniques in selecting a problem.
Unit II – Research Design

Research design: meaning need, research designs – Basics of experimental designs – Developing
research plan.
Unit III – Literature Survey
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Source of literature – INSDOC, MEDLINE, Biological abstracts, Current Contents, Pubmed and
Online Journals – Types of literature: reviews, abstracts, short notes, journal articles, magazines,
periodicals, books and proceedings – Reprint requisition cards and reprint requesting –
Organization of collected information – index cards and style of indexing
Unit IV –Data collection

Methods of data collection- Questionnaires/schedule, Selection of appropriate method for data
collection – Guidelines for constructing questionnaires – Guidelines for interviewing – Sample
questionnaires – Data Analysis and interpretation – Dissertation /Reporting – Steps in report
writing – How to critique an article.
Unit V – Publication
Preparation of research reports, project reports, scientific report writing – Documentation: Library
cataloguing, definition, need, objectives and function of catalogue (Colon Classification, Dewey,
ISBS, ISSN, Bar Coding and Accession Number) – Internet Resources: What is Internet, World
Wide Web, Navigating the Internet, Electronic mail, and Electronic publication, Online Journals
– Structure and ethics of Scientific presentations.
Unit VI – Funding resources and designing research proposals

World Health organization (WHO), Department of Science and Technology (DST), Indian
Council for Medical Research (ICMR) – Structure of Research Proposals: Origin, Objectives,
Existing Knowledge of the problem, Current status of the problem, Methodology, Budget and
expected outcome.
Reference Books:
1. Doing Your Masters Dissertation, Chris H, 1st edition , Vistaar publications, 2005
2. Research Methodology, Paliniswamy and Shanmugavel.
3. Research Methodology Methods & Techniques, C. R. Kothari.
M.Sc. (vi) - SEMESTER II - PAPER 8 : MAJOR CORE
DEVELOPMENTAL BIOLOGY 5 Hours / week 4

credits
(Objective: To elaborate the Scope of development biology and its development as science and a
technology)
Unit I
Gametogenesis Spermatogenesis and Oogenesis – role of Hypothalamo hypophyseal Gonadal
axis. Types of gametes in animals – the developing egg and its environment – sexual cycles
(estrus and menstrual) – role of hormones in gametogenesis.
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Unit II
Fertilization and Cleavage – physico-chemical events related to fertilization and cleavage –
morphological aspects of gastrulation and primary organ formation – physiology of gastrulation.
Unit III
Determination of primary organ rudiments. Embryonic adaptations.
Unit V
Organogenesis – morphogenetic process – development of central nervous system, heart and
alimentary canal.
Unit VI
Differentiation and growth – general considerations.
Unit VII
Metamorphosis – regeneration and sexual reproduction
Unit VIII
Reproductive technology: Synchronisation of estrus – control of ovulation embryo transplantation
and storage – diagnosis of pregnancy – induction of parturition – out of season and prepuberal
breeding – selection technique and sex pre determination – cloning.
Reference Books:
Introduction to Embryology, Balinsky

Developmental Physiology, Raven
Reproduction in farm animals – Hafez
Reproduction in Farm animals, R.H.F. Hunter.
M.Sc. (vi) – SEMESTER II - PAPER 9: M AJOR CORE PRACTICAL – II

BIOINFORMATICS + BIOSTATISTICS 3 hours / week 2 Credits
(for students admitted from 20014-15 onwards)
Bioinformatics
1. Basics of Bioinformatics, types of data basse, Online sequence retrieval- NCBI.

2. Literature database- Pub Med, Medline, OMIM.
3. Sequence database- Protein- UniProt, Prosite, Pfam, Nucleotide- Gen Bank.
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4. Homology study- Basic alignment- BLAST & FASTA.
5. DNA & protein sequence analysis, pair wise (Emboss) alignment techniques.
6. Multiple alignment techniques -Clustal W.
7. Structural Database: - PDB, Protein structure Visualization- PyMol, SPDV.
8. Secondary databases -SCOP, CATH.
9. Metabolic pathway database- KEGG.
10. Docking- Argus lab.
11. Protein Modeller.
12. SAVS ( Ramachandran plot validation)
Biostatistics
1. Representation of data in one, two, three dimensional diagrams and pictograms.

2. Graphic representation of data through graphs of time series and frequency
distribution.
3. Statistical software package SPSS- Descriptive Statistics, Correlation and regression,
ANOVA- one way and two way
M.Sc. (vi) – SEMESTER II - : MAJOR CORE PRACTICAL – IV

DEVELOPMENTAL BIOLOGY& ENTOMOLOGY 2+3 hours / week 3 Credits
1. Semen collection from Bull.
2. Sperm count.
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3. Egg diameter measurement, volume and density of microscopic eggs.
4. Mounting of chick embryos.
5. Study of the developmental stages of chick embryo-24,36,48,60,72,84,96 hours.(any5)
6. Observation of developmental stages of frog and Amphioxus (slides)
7. Dissection of tadpoles to show the anatomical peculiarities and transitions.
8. Regeneration studies in tadpoles/house lizard.
9. Study of placentation in goat/pig and human.
10. Oviparity, ovoviviparity and parental care (spotters)
General Entomology
Dissection of digestive nervous and reproductive systems. Mounting of mouth parts and other
special morphological modifications of the following species: Lepisma, Earwig, Ant lion,
Naiad of Dragon fly, Adult Dragon fly, Anthia, Laccotrephes or Ranatra, Calliphora, Apis,
Polistes, Butterfly
a. Collection, pinning and preservation of Insects
b. Identification of some common insects or their damages on MCC (at least 20 insects)
c. Submission of record and insect box
Applied Entomology
a. Collection, identification and study of any four insect pests of crops grown in MCC
farm – Paddy, vegetables – Brinjal and Okra
b. Identification of four insect pests of mango
c. Identification of any four stored product insect pests.
d. Identification of beneficial insects: Honey bee, Silk worm larval stage and cocoon,
chandraki
e. Insects of medical importance – mosquito, housefly and headlouse
f. Insecticides : Knowledge of any organochlorine- DDT/HCH, Organophosphorous –
Monocrotophos/ Chloropyriphos, Carbamate – Carbofuran, Synthetic pyretheroid –
Fenvalerate / Cypermethrin
g. Knowledge of field layout to study the efficacy of insecticides.
h. Visit to a biological control laboratory to study the rearing of biocontrol agents
i. Submission of record
M.Sc. (vi) - SEMESTER III - PAPER 10 : MAJOR CORE

ANIMAL PHYSIOLOGY & BIOCHEMISTRY 3+3 Hours / week 4
Credits
63
Animal Physiology
Unit- I Osmotic and ionic regulation (aquatic & terrestrial ) :
Maintenance of osmotic and ionic balance in animals living in hypo, hyper and iso-osmotic
environment.
Unit- II Body fluids and their circulation:

Plasma proteins and regulation of fluid volume.
Cardiac rhythm- Open and closed system- Vascular pump- chambered heart and booster pumps-
Pattern of circulation of Vertebrates- The lymph hearts of Vertebrates- Pace makers of myogenic
hearts- The rhythm of neurogenic heart- Blood pressure and Haemodynamics.
Unit- III Neuromuscular and neuroendocrine integration and regulation:

Myoneuronal junctions- Patterns of efferent innervation of muscles- Motor units in vertebrate
muscle- Fast and slow contractions- peripheral inhibition- Neuromuscular transmitters.
Neurosecretory system- Mechanism of hormone actions (Steroid hormones and cascade
amplification system).
Unit- IV
Electric organs: Morphology- physiology
Bioluminescence: Luminescent organs- distribution of bioluminescence- biochemistry of
luminescence (firefly- cypridina - coelenterate). Bacterial, extracellular and intracellular
luminescence- functional significance of bioluminescence. Sound Production and reception
– Communication- Echo-orientation.
Reference Books:
1. Hoar- General and Comparative Physiology
2. Wilson- Animal Physiology
3. Prosser and Brown- Animal Physiology
4. Tuttle & Schottelius – Text book of Physiology
5. Kunt and Schmidt Nielson - Animal Physiology
6. Potts and Parry – Osmotic and Ionic regulation.
7. Lehninger – Principles of Biochemistry.
Biochemistry
Unit I: Enzymes: Classification, mechanism of enzyme action, Michaelis-Menton reaction,

enzyme inhibitors.
Unit II: Anabolism – biosynthesis: Carbohydrates: central pathway, bypass reactions,

gluconeogenesis, glycogenesis. Lipids – Mitochondrial synthesis of fatty acids, non-mitochondrial
synthesis of fatty acids, synthesis of triglycerides, synthesis of phospholipids, synthesis of
cholesterol, errors in lipid metabolism.
Unit III: Catabolism: Carbohydrates – glycolysis, TCA cycle, HMP pathway, glycogenolysis,
energetics of the cycles. Proteins – overview of protein catabolism – Transmination, deamination,
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fate of ammonia and carbon skeleton, urea cycle. Lipids – mobilization of fats from dietary
intake. Break down of fatty acid and cholesterol.
Unit IV: Integration of biomolecules - intermediary metabolites – phosphorylation, types,

biological oxidation, energy rich compounds – oxidative phosphorylation.
Reference Books:
Jain, L.L. Sunjay Jain & Nitin Jain 2005. Fundamental of biochemistry, S. Chand and Company
Ltd., New Delhi, pp.1230.
Ambika Shanmugam 1974. Fundamentals of Biochemistry for Medical Studies. Second Edition
(Revised), Aries Agencies, Chennai, pp.647.
West, Edward Staunton, Todd Wilbert R. Mason Howard, S. and Bruggen John T. Van. 1974.
Textbook of biochemistry Amerind Publishing Co. Pvt. Ltd, New Delhi, pp.1595.
Wilson, Jean, D. Foster Daniel W., Kronenberg Henry M and Larsen Reed, P. 1998. Williams Test
book of Endocrinology. W.B. Saunders Company, London, p.1819.
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M.Sc. (vi) - SEMESTER III - PAPER 11- MAJOR CORE THEORY
MICROBOLOGY & IMMUNOLOGY 3+3 Hours / week 4
Credits
Microbiology
Objective: To expose the students to various micro-organisms and their applied aspects.
UNIT I
History of Microbiology. Discovery of the microbial world.
UNIT II
Bacterial identification. nomenclature and classification, New approaches to bacterial
taxonomy / classification including ribotyping and ribosomal RNA sequencing
UNIT III
General structure and features, brief account of all group of bacteria and cyanobacteria,
Rickettsia, Chlamydia and Mycoplasma
Archaea : Archaebacteria and extremophilic microbes – their biotechnological potentials
UNIT IV
The definition of growth, growth curve, measurement of growth and growth yields, Culture
collection and maintenance of cultures. Isolation, pure culture techniques, Methods of
sterilization.
UNIT V
Different modes of nutrition in bacteria, Sulfate reduction, Nitrogen metabolism – nitrate
reduction, nitrifying and denitrifying bacteria, Nitrogen fixation and Microbes used as
biofertilizer
UNIT VI
Viruses : Classification, morphology and composition of viruses in general,
Bacteriophages : φX 174, cyanophages and retroviruses, Viroids and Prions
UNIT VII
Applications in Microbiology:- Industrial Microbiology: Microbes in the production of alcohol
and antibiotics.
UNIT VIII
Dairy microbiology: Microbiology of milk and milk products,
UNIT IX
Agricultural microbiology: Nitrogen cycle, phosphorous cycle, nitrogen fixation, soil fertility and
biogas. Medical Microbiology – study of common bacterial (10), fungal (5) and viral (5) diseases
of man and their preventive measures.
UNIT X
66
Microbiology of drinking water. Methodology of bacterial analysis of water- water borne
diseases- Sewage- biological sewage purification methods- Chlorination of water and its
implication.
Reference Books:
Microbiology – Anna K. Joshua
Microbiology – Norten C.F.
Microbiology – Pelcazar, Reid and Chan
Text Book of Microbiology – Ananthanarayanan and Jayaram
Food Microbiology – Frazier
Industrial Microbiology – Casida L.F
Micro biology of water and SewageGeinyl and lord
General Microbiology – Boyd
Microbiology – Atlas – Biology of Microorganisms – Brock and Madigan
General Microbiology – Stainer, John, Mark
Microbiology - Zinsser
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Immunology
Unit I : Introduction to immunology

History of development of immunology and scope. Classification of immunity – natural, induced,
innate, acquired, specific, non-specific. Immune response-primary and secondary and
Heideberger-Kendall reaction.
Unit II: Lymphoidal organs

Primary bone marrow, thymus, Bursa of fabricius, secondary spleen GALT, MALT. Peyer’s
patches, Kupffer cells, etc. – Theory locations structure and function.
Unit III: Principles of classification

Stem cells and differentiation and classification of lymphoid cells.
Unit IV: Fundamentals of immunity

Immunoglobulins – structure, classification, theories of formations and functions. Separation of T
and B lymphocytes. Antibody-mediated and cell-mediated immunity. Cytokines. Antigen
presentation. Compliment system. Major histocompatibility complex. Phagocytosis. Tolerance.
Unit V: Immunity of viral, bacterial, fungal, protozoan, and helminth infections

Natural response and immunological mechanism against such infectious agents; immune
mechanisms of host.
Unit VI: Primary and secondary immunodeficiency states

Occurrence of primary and secondary immunodeficiencies with examples.
Unit VII: Hypersensitivity Reaction

Types I, II, III, IV and V. Roll of immunoglobulins in various types of reactions; Reactions and
responses of the system and tests; specific examples for each type. Disease manifestation and
modern treatment methods.
Unit VIII: Transplantation and Rejection

Tissue typing and HLA system: (Genetics); grafting and classificationof grafts; graft rejection and
prevention and role of T. Lymphocytes; immunosuppression, immunomodulators.
Unit IX: Autoimmunity

Types of autoimmune diseases, antibodies, occurrence, genetic factors and pathogenicity
diagnostic and prognostic values.
Unit X: Modern trends of immunology

Biotechnological trends in organ / tissue grafting technology, vaccinology; Bispecific antibodies;
monoclonal antibodies, vaccines through food. Role of natural research laboratories and institutes.
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Reference Books:
Immunology by Roiit.
Basic immunology by Gupta
Fundamentals of immunology by Weir
Immunology by Barrett.
M.Sc. (vi) - SEMESTER III - PAPER 12: MAJOR CORE

ENVIRONMENTAL BIOLOGY & BIODIVERSITY 4+2 Hours / week 4
Credits
Environmental Biology
UNIT I - ECOSYSTEM ECOLOGY: Ecosystem components; concept (Aquatic,Terrestial,
Deep Sea, Polar, Micro and Space ecosystem): Biological control of the chemical environment:
Production and decomposition in nature: Homeostasis.
UNIT II - ENERGY in ECOSYSTEM : Energy and Environment : Concept of productivity.
UNIT III - SPECIES in ECOSYSTEM : Concepts of habitat and ecological niche; ecological
equivalents; natural selection and speciation: Biological clocks: Ecosystem development and
succession.
UNIT IV - RESOURCES : Forest, grass land and agriculture; capture and culture fisheries;
Land, soil and water; fossil fuel; minerals; wild life; energy of biological origin and non-
conventional energy resources.
UNIT V- RESOURCE MANAGEMENT : Conservation of biodiversity; renewable and non-

renewable; deforestation and aforestation; wild life conservation; land use; desalination and
weather modifications; national programmes on conservation.
UNIT VI - POLLUTION and ENVIRONMENTAL HEALTH : Air, water and soil pollution;
special emphasis on CO2, CO, pesticides and heavy metals (lead, mercury, chromium): Solid
wastes and their management; Agro-industries, Thermal, Tanneries pollution: Noise pollution:
Pollution as it relates to meterology and climatology; el-nino: Sewage treatment: Environmental
laws to monitor pollution.
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UNIT VII - ENVIRONMENTAL EDUCATION AND ORGANIZATIONS : Goals,
objectives and guiding principles; various programmes in India; Environmental organization and
agencies: Environmental issues:- silent valley, Narmadha: International bodies: Man and the
biosphere programme: National organization:- Department of environment, forest and wild life;
Important abbreviations/acronyms.(AEC, BSI, CFC, CPCB, CRZ, DNES, DST, EIA, ENVIS,
EPA, EPC, FAO, GEMS, IUCN, MAB, NEERI, NEPA, SPCB, UNESCO, WHO, WWF-INDIA).
UNIT VIII - BIOREMEDIATION : Defintion; Need and scope; Environmental application;

Future outlook; Phytoremediation:– biotechnology of cleaning up the environment by plants.
UNIT IX - SPACE ECOLOGY : Types of life support systems in space; exobiology; hazards
of space travel.
UNIT X - ENVIRONMENT AND FUTURE OF BIOSPHERE
A. GLOBAL WARMING : Causes:- Co2, Methane, Chloroflurocarbons, Nitrous oxide, Ozone.

Consequences:- climatic change, human diseases. Impact:- on forests, Agriculture.
B. POPULATION GROWTH AND FOOD PRODUCTION : World population:- growth,

regulation, modern technology and population explosion, future of world population, population
growth in India.
World food production:- Green revolution, towards second green revolution.
C. URBANIZATION AND ITS IMPACT ON SOCIETY : Population growth and urbanization:

Factors contributing urbanization:- migration, industrialization, transportation, socio-economic
changes: Impact on environment, health, society: Management of urbanization.
D. ENVIRONMENTAL IMPACT ASSESMENT : Process and prepation of environmental

impact statement, cost benefit analysis, current status EIA in developed countries(U.S.A.,Canada,
Germany, U.K., Japan), and developing countries (India, Indonesia, Philippines). GIS; remote
sensing.
E. ENVIRONMENT AND SUSTAINABLE DEVELOPMENT : Sustainable growth in the

context of Population growth, Urbanisation, Industrial growth, global warming, military conflicts
and nuclear war. Biotechnology and nature management as a way to sustainable development:
People’s participation: Future Scenario.
Reference Books:
Fundamentals of Ecology – Eugene P Odum 1991.W.B.Saunders Company.
Fundamentals of Environmental Sciences – G.S.Dhalival, G.S.Sangha, P.K.Ralhan. 2000
Kalyani Publishers.
Ecology and Environment – P.D.Sharma 1999, Rastogi Publications.
Animal Ecology and Environmental Biology – H.R.singh 2001, Shoban Lal Nagin Chad & co
Biodiversity
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UNIT – I
Defining Biodiversity Concept, Convention on Biological diversity – role, goals, functions and
perspectives. Global biodiversity assessment
UNIT – II
Biodiversity from taxonomic and evolutionary perspectives; Origin of scientific taxonomy, basis
of taxonomic characterisation, characterizing species (the morphological species concept,
biological species concept, phylogenetic species concept) Phylocode classification. Taxonomic
measures of species diversity, modern developments (database and expert identification systems).
UNIT - III
Types of diversity – Ecosystem diversity (Variety and habitat), Species diversity, Genetic
diversity, Methods of measuring species diversity and genetic diversity, Causes of biodiversity
loss, Effects of biodiversity loss.
UNIT-IV
Human Society and biodiversity- Cultural diversity, Values of biodiversity, People’s movement,
Climate Change, Conservation – Approaches and Sustainable development.
UNIT V
Mega diverse nations, Hot Spots of biodiversity, IUCN categories of threat, Biosphere Reserves
and Conserved areas of India, Projects to Save Threatened species.
UNIT VI
National Biodiversity Act- policy and action, Wild life Regulation Acts, National and
International Treaties and Conventions, Intellectual Property Rights over Biological products
Reference Books:
1. Ferguson. C. 1980. Biochemical Systematics and Evolution.

2. Mayor. E. 1994. Principles of Systematic Zoology.
3. Agarwal. K.C. 2000. Biodiversity.
4. A.B.Choudhari and D.D.Sarkar 2002. Biodiversity Endangered.
5. Kumar.V. 2003. Biodiversity Principles and Conservation.
6. Kotwal P.C. 2003. Biodiversity Conservation in Managed Forests and Protected Area.
7. Trivedi. P.C. 2004. Biodiversity Assessment and Conservation.
8. India 2006: Ministry of Information and Broadcasting, Government of India, New Delhi.
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M.Sc. (vi) - SEMESTER III - PAPER 13: MAJOR CORE PRACTICAL – III
ANIMAL PHYSIOLOGY, BIOCHEMISTRY & IMMUNOLOGY 2+2+2 Hours / week 3
credits
Animal Physiology
1. Preparation of Maltose standard graph

2. Human Salivary Amylase activity in relation to Temperature
3. Rate of activity in relation to body mass / weight in an aquatic organism (Fish)
4. Estimation of Haemoglobin
5. Estimation of ESR
Biochemistry
1. Paper Chromatography.
2. Thin layer chromatography.
3. Quantitative Estimation of Blood Glucose.
4. Quantitative Estimation of Total Protein.
5. Quantitative Estimation of HDL, LDL (Triglycerides).
6. Quantitative Estimation of Creatinine in urine.
7. Quantitative Estimation of Creatinine in serum.
8. Quantitative Estimation of Urea in serum.
9. Quantitative Estimation of Urea in urine.
10. Quantitative Estimation of Serum cholesterol.
11. Quantitative Estimation of Alkaline phosphate
Immunology
1. Precipitin Test
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2. Dissection to locate primary and secondary lymphoid organs (any vertebrate)
3. Vaccination Schedule and vaccines used.
4. Demonstration of immunization (Intravenous,Intraperitoneal, cutaneous,ocular,oral)
5. Observation of permanent slides of lymphoid organs / tissues.
6. Radial Immunodiffusion and Ouchterlony double diffusion using Agarose, Aleiver’s.
7. Fluorescent staining of blood for observation of lymphoid cells.
8. ELISA-demo.
9. Report of survey of awareness of HIV/AIDS amongst students.
Visit to Institute and departments related to immunology and vaccine producing laboratories-
Diagnostic centre.
M.Sc. (vi) - SEMESTER III - PAPER 13: MAJOR CORE PRACTICAL – III
ENVIRONMENTAL BIOLOGY & BIODIVERSITY AND MICROBIOLOGY 2+2 Hours /
week 2 Credits
Environmental Biology and Biodiversity
1. Qualitative and quantitative analysis of plankton sample.

2. Determination of hardness of water – calcium, magnesium.
3. Estimation of BOD in pond water and polluted water.
4. Study of soil characteristics:- colour, texture, moisture, pH, carbonate and nitrate.
5. Study of terrestrial community by quadrat method :- determining frequency, density and
abundance of different species present in the community.
6. Measurement of particulate air pollutants - dust fall and suspended particulate matter from
different areas.
7. Visit to sandy shore, muddy shore and rocky shore to collect and study the organisms and
their adaptation to the environment.
8. Study of pond / estuarine ecosystem (Estuarine Biological Laboratory, Pulicat): Plankton
collection, estimation of primary productivity, PH, salinity, dissolved oxygen content, free
carbon dioxide content, analysis of biotic community and construction of food chain and
food web.
9. To Study the biodiversity of fauna of the scrub jungle of MCC campus and to estimate
Absolute density - Relative dominance - Relative density - Relative frequency - Simpson,
Index of dominance - Community dominance index - Community similarities - Coefficient
of community - Species diversity index - Index of frequency of occurrence together and
Species association test
10. Students may be exposed to the following areas (not for assessment)-sewage treatment
plants; drinking water treatment plants; atomic power plants; refineries and tanneries.
Microbiology
73
1. Commercial kits-based diagnosis.
2. Antibiotic sensitivity(bacterial).
3. Bacterial culture
4. Agar gel diffusion
5. ELISA
6. Preparation of axenic cultures
7. Laboratory Safety, Microscopy, Aseptic Technique
8. Bacterial Cultures, Slide Preparation, Staining, Streaking
9. Streaking, Pour Plates
10. Bacterial Characteristics
11. Differential and Selective Media
12. Bacterial Identification
13. Litmus Milk, Carbohydrate Fermentation, Hydrogen Sulfide and Agglutination Tests
M.Sc. (vi) - SEMESTER IV - PAPER 14 : MAJOR CORE

BIOTECHNOLOGY 6 Hours / week 4
Credits
Unit – 1: Introduction: Historical perspective; definitions; scope of biotechnology;

commercialization of biotechnology. Fundamentals of molecular biology. 5 hrs.
Unit – 2: Gene technology: Restriction endonucleases as tools for DNA fragment generation;
linkers and adaptors; expression of cloned genes; P.C.R.; vectors; rDNA technology; transgenics;
cloning; DNA sequencing; gene libraries; human genome project 10 hrs..
Unit – 3: Protein engineering and design: Protein structure and function; methods in protein
engineering and design; production of foreign proteins. Gene therapy. 8 hrs.
Unit – 4: Enzyme technology: Traditional uses of enzymes; immobilization – methods and uses;
biosensors. 5 hrs.
Unit – 5: Bioprocess technology: Exploitation of micro-organisms and cells; S.C.P.; mining; over-
exploitation in various fields of biotechnology. 8 hrs.
Unit – 6: Human health and biotechnology: Application of biotechnology in the field of
biotechnology – diagnostics and therapy; human protein replacements; vaccines; A.R.T.;
monoclonal antibody technology; immunotechnology; human gene banks; related ethical issues.
12 hrs.
Unit – 7: Agricultural biotechnology: tissue culture; micropropagation; somatic hybridization;
transgenic plants. 4 hrs.
Unit – 8: Animal biotechnology: transgenic animals; high yielding and disease resistant animals;
A.R.T. in animal husbandry; cloning and transgenic animals; animals as models and related
ethical issues. Fishery biotechnology. 10 hrs.
74
Unit – 9: Environmental biotechnology: environmenmt and human health; biomonitoring of
pollution; waste treatment; bioremediation; global environmental problems. Bioethics. I.P.R. and
patents. 8 hrs.
Reference Books:
1. Molecular biology of the gene. Watson, et al.
2. Concepts of Biotechnology. Balasubramanian, et al.
3. Biotechnology. U. Sathyamurthy.
4. Animal Biotechnology. Ramadas.
5. Introduction to Biotechnology. Gupta.
6. Basics in Biotechnology – Springer Verlag
M.Sc. (vi) - SEMESTER IV - PAPER 15 : MAJOR CORE

MOLECULAR ENDOCRINOLOGY 5 Hours /week
4credits
Unit I: Role of Hormones

1. Hormone biosynthesis and secretion
2. Transport of hormones
3. Mechanism of hormone action: Peptide and steroids
4. Receptor types and its mode of action
Unit II: Endocrine manipulation of Reproduction in Mammals

1. Overview of hormonal control of reproduction in mammals
2. Manipulation of the estrous cycle
3. Induction and synchronization of estrus
4. Superovulation and embryo transfer
5. Hormones in maintenance of pregnancy
6. Induction of Parturition
7. Inducing puberty
Unit III: Gonads and fertility disorder

1. Amenorrhea
2. Polycystic ovary
3. Hirsutism
4. Cryptorchism
5. Varicocoele
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6. Sertoli cell syndrome
Unit IV : Gamete Preservation in Fish & Mammals

1. Sperm motility and metabolism
2. Gamete quality
3. Short-term preservation of spermatozoa
4. Short-term preservation of ova
5. Cryopreservation of gametes
Unit V: Anabolic steroids and Analogues

1. Direct and indirect effects
2. Delivery systems
3. Effects of sex steroids on meat quality
4. Safety issues
Unit VI: Hormonal response to stress

1. Sympatho-adrenal system
2. Hypothalamic – Pituitary axis
3. Role of various hormones in stress responses
4. Effects of stress on reproduction
5. Effects on growth performance
Unit VII: Obesity

1. Cause and Consequences
2. Outline of hormonal involvement
3. Leptin: synthesis, secretion and its role in adipogenesis
Unit VIII: Thyroid disorders and management

1. Antithyroid drugs, dietary goitrogens
2. Goiter
3. Myxedema
4. Cretinism
Unit IX : Insulin and Glucose balance

1. Glucose homeostasis
2. Insulin resistance & Management
3. Diabetes mellitus & Management
Reference Books:
1. G. M. Besser & M. O. Thorner. Comprehensive Clinical Endocrinology, IIIrd Ed. (2002),
Mosby.
2. E. knobil & D. Niell. Encyclopedia of Reproduction (1998), Academic press.
3. P. J. Bentley. Comparative Vertebrate Endocrinology, IIIrd (1998), Cambridge University
Press
4. E. J. Squires. Applied Animal Endocrinology (2003), CABI Publications UK.
5. Mac E. Hadley. Endocrinology, V Ed. (2000), Prentice Hall International Inc.
6. Norris. Vertebrate Endocrinology, IIIrd ed. (1998). Lea & Febiger.
76
7. P. R. Larsson et.al., Williams Text Book of Endocrinology, Xth Ed. (2002), W.B. Saunders,
Philadelphia.
77
M.Sc. (vi) – SEMESTER IV - PAPER 16: MAJOR CORE PRACTICAL - IV
BIOTECHNOLOGY AND ENDOCRINOLOGY 3+3 Hours / week
4Credits
Biotechnology
1. Design of bioreactor.
2. Electrophoretic separation of proteins (PAGE)
3. Genomic DNA isolation from animal tissues.
4. Detection of DNA.
5. Quantitative estimation of DNA.
6. Enzyme immobilization.
7. Chromatographic techniques – Column Chromatography
8. P.C.R.
9. Principles of animal cell culture.
10. ELISA.
11. Immunoblotting.
Endocrinology
1. Dissection to see various endocrine glands in mice/Rat, frog / Wall lizard

2. Observation of histological slides: Pituitary, Thyroid, Adrenal, Pancreas, Thymus,
Ovary, Testis and Epidydimis
3. Ectomy experiments: Adrenalectomy, Castration, Ovrectomy in lab mice (any one)
4. GTT experiments
5. Thyroxin test in Tadpoles
6. Estrous cycle study by vaginal smears in mice
7. hCG based pregnancy test
78
M.Sc. (vi) – SEMESTER IV -: MAJOR ELECTIVES: PAPER 17
ANIMAL BEHAVIOUR 4 Hours / week 5
Credits
Unit I Introduction: Definition (ethology, instinct –learning, Altruism, agnostic behaviour, derive,
displacement behaviour, territorial behaviour, communication, hierarchy, FAP, stimuli, ethogram,
Time budgets) Lorenz’s and tinbergen’s models for release of FAP, modern methods in the study
of behaviour.
Unit II Describing behaviour: Description by form and function, Advantages and disadvantages,
methods of data collection – field observations, capture, recapture, camera tagging, radioelemetry,
lab experiments, activity recordings.
Unit III: Living groups: Costs and benefits, communication (types, design of signals, variability
in signals – bees, birds and bats), behaviour expressed in a group (territorial, altruism, courtship
and mating, agnostic hierarchy).
Unit IV: Rhythmic behaviour: Kinds of biological rhythms (circadian, tidal, lunar and annual).
Characteristics of circadian rhythms, terminology used (Zeitgeber, phase, phase shifts, phase
angle difference, entertainment, freerunning rhythm, CT, PRC, Actogram), biological clocks.
Unit V: Behavioural Genetics: Single genes and behaviour, chromosomal mutations affecting
behaviour, polygenic inheritance of behaviour, behaviour of inbred, recombinant strains,
molecular basis of circadian rhythms, phylogeny of behaviour.
Unit VI: Neural control of Behaviour: Language of the nervous system, brain centers and their
functions, learning and memory, hormones and behaviour.
Unit VII: Applied Ethology: Pest control, managing population size, improving productivity
promoting the welfare of farm and captive animals, wildlife management.
Unit VIII: Human behaviour

Human actions (innate, discovered, absorbed, trained and mixed), gestures (primary and
secondary), greeting signals, baron signals.
Reference Books:
McFarland, D. Companion to Animal Behaviour
Manning, Introduction to Animal Behaviour
Desmond Morris, Manwatching.
79
M.Sc. (vi) – SEMESTER IV -: MAJOR ELECTIVES: PAPER 17
AQUACULTURE 4 Hours / week 5
Credits
Objectives: To introduce the knowledge of aquaculture potential and practices in India, and to
realize the need for augmenting food production from the aquatic resources through aquaculture
Unit I
Scope and definition. Nature and socio-economic basis. Biological and technological basis.
History of aquaculture and its present status at the National and Global level. National and
International research and development organizations.
Unit II
Classification of cultivable aquatic organisms of India (taxonomic and ecological) pre-requisites
for cultivable organisms.
Unit III
Basic considerations in aquaculture. Definition of traditional extensive and intensive, semi-
intensive culture practices; preparation and management of ponds – construction, fertilization,
control of aquatic insects, weeds and predatory and weed fishes. Estimation of live-feed
organisms. Procurement of seed and its transportation natural and artificial feeds, nutritional
ecology (Energy budgets). Spawning and breeding, parasites and diseases.
Unit IV
Productivity and plankton. Methods of productivity studies in an aquatic environment; qualitative
and quantitative studies of plankton; culture techniques of phyto and zooplankters.
Unit V
Culture Techniques : Sea-weed culture. Resources and utilization; culture techniques.
Unit VI
Shell fish culture: Crustacean culture. Shrimp culture, pattern of life-cycles of penaeids and non-
poenaeids , induced maturation and breeding of cultivable shrimps- Formulated feeds of shrimps
and prawns and lobsters. Recent breakthroughs in the induced breeding of shrimp, prawns and
crabs. Biology and culture techniques.
Unit VII
Fin-fish culture
Patterns of life cycles of brackishwater cultivable fin fishes. Techniques of induced breeding of
cultivable fin-fishes. Monoculture, polyculture, special culture techniques of various freshwater,
brackishwater, marine finfishes viz., pond, cage, pen, race-way, recirculatory, paddy-cum-fish
80
culture, sewage-fed fishculture, sustainable aquaculture, culture based fisheries of small
reservoirs, flood plain wetland culture fisheries close to jheels. Lab- to -land programmes.
Unit VIII
Constraints of aquaculture (i) low quality and costly feed ingredients, (ii) weak extension
network, (iii) lack of field exposure, (iv)insufficient R & D support (v) lack of proper database
(vi) non-availability of proper insurance schemes (vii) lack of policy for aquaculture.
Reference Books:
Reay, R.J. 1979, Aquaculture. Edward Arnold (Publishers) Ltd., pp.56.
T.V.R. Pillai, 1993. Aquaculture – principles and practices fishing news publication, pp.578.
Sinha, V.R.P. 1993. A compendium of aquaculture technologies for developing countries, Oxford
I.B.P. Publishing Co. Pvt. Ltd., pp.115.
Annon, 1995, Freshwater fishes Hand Book on aquaculture, MPEDA, Cochin, pp.102.
T.J.Pandian (ed.) 2001. Sustainable fisheries. National academy of agriculture sciences, pp.327.
81
M.Sc. (vi) – SEMESTER IV- MAJOR ELECTIVE : PAPER 18
TEACHING ZOOLOGY 4 Hours / week 5
Credits
UNIT I : Introduction
Nature and scope of Life Sciences in Modern times – trends in teaching life sciences – aims and
objectives with reference to Bloom’s taxonomy – teaching life sciences at different levels.
UNIT II: Microteaching:

Teaching skills – demonstration – explaining – Black board writing – skills of stimulus variation –
questioning – uses of aids and reinforcement.
UNIT III: Lesson Planning:

Principles of lesson planning – its importance – approach to lesson plan – planning for specific
behavioural changes – Herbartian steps – its merits and demerits.
UNIT IV : Education Technology:

Traditional: importance of aids in teaching – principles and preparation of charts, pictures,
models, objects, specimen, transparencies, audio and video tapes – magnifying equipments like
microscopes – preservation of biological specimens including herbarium, cultures, aquarium,
vivarium, aviary, museum – setting up a lab – accidents and first aid.
Modern Technology: Flow chart – computer aided learning – internet search – use of multimedia.
UNIT V: Methods of teaching:

Inductive and deductive approach – Importance of formulation and verification of hypothesis –
General methods of Teaching: lecture – Lecture cum demonstration – practical project –
PowerPoint presentation – Seminars – group discussion – field trip – organizing seminars and
symposia.
UNIT VI: Evaluation:

Achievement tools – preparation of question paper – essay type, objective and short answer – blue
print of question paper – construction and use of tests – statistical analysis of results and
performance – diagnostic tests – remedial measures – teaching and student evaluation.
Reference Books:
Secondary School Teaching Methods
By Leonard H. Clark, Irving S. Starr
Exercises for "Methods of Teaching in High Schools":

Samuel Chester Parker
82
Science Teaching Methods
Harold R. Hungerford, A. Tomera
A Method of Teaching Zoology

By Elliot Whipple
M.Sc. (vi) – SEMESTER IV- MAJOR ELECTIVE : PAPER 18

MEDICAL LABORATORY TECHNIQUES 4 Hours / week 5
Credits
UNIT I: Human Anatomy and Physiology:

Organs and organ systems – levels of organization – study of the following systems in detail:
circulation – digestion – urinary – endocrine.
UNIT II: Lab organization, maintenance and instrumentation:

Code of ethics – organization of Laboratory - laboratory administration - maintenance of records
and reporting – construction – care and use of the following equipments: photoelectric
colorimeter, spectrophotometer, ECG apparatus, electrophoresis, Biochemical analysers and
ELISA Reader.
UNIT III: Medical parasitology:

Morphology, life cycle, mode of infection, pathogenesis, prophylaxis and treatment of the
following parasites found in human.
Protozoology: Rhizopoda – Entamoeba histolytica, Entamoeba coli, Sporozoa – Plasmodium sp.,
Ciliata – Blantidium coli and flagellates – Trichomonas, Trypanosoma, Giardia lamblia.
Helminthology: Nematodes – Ascaris, Hookworm, Enterobius, Trichuris, Wuchereria.
Cestodes – Taenia saginata, Taenia solium, Hymenolepis nana, Diphylobothrium latum.
Trematodes – Common Blood flukes, Intestinal and Lung flukes and Liver flukes.
UNIT IV: Clinical immunology

Cell mediated and humoral immunity; Immuno diagnostic test.
UNIT V : Biochemistry and techniques in Histopathology:

Enzymes: Basic concepts, clinically important enzymes – metabolic disorders.
Use of enzymes, immobilization of enzymes and their application in biotechnology.
Preparation and processing of tissues – Fixation / dehydration / impregnation with paraffin wax /
embedding / block making / section cutting / mounting and staining.
UNIT VI: Haematology and Clinical pathology

Blood – composition – functions – production of cells – Types of anaemias – clotting mechanisms
and disorders. Blood groups and blood banking – study of body fluids – sputum. Faeces, semen,
83
gastro and duodenal contents – cerebrospinal fluid, transudates and exudates – basic information
of commitment.
Reference Books:
A text book of physiological chemistry, Harold Harper.
A text book of biotechnology, Kumar
Anatomy and Physiology for Nurses, Evelyn and Pearce
Biochemistry, Ambika Shanmugam
Biotechnology – The biological principles: Treven, et al.
Cell biology: De Robertis
Essential Immunology: Roit
Human Anatomy and Physiology: Best and Taylor
Immunology: Weir
Instrumentation techniques: Griffith
Introduction to Human Anatomy and Physiology: Eldra Pearl Solomon
Language of Medicine: David Ellen Chabner
Manual on Medical Lab Technology: CMC Medical Foundation
Medical Parasitology: Jayaram Panicker
Medical Physiology: Guyton
Medical Microbiology: Anantanarayanan and Panicker
Notes on Clinical Lab Techniqes: K.M. Samuel
Practical Clinical Biochemistry: Herold Varley
Textbook of Medical Laboratory Technology: Ramnik Sood
Textbook of Medical Laboratory Technology: Kanai Mukherjee
Textbook of Biochemistry: Lehninger
84
M.Sc. (vi) – SEMESTER IV- MAJOR ELECTIVE : PAPER 19 PRACTICAL V
ANIMAL BEHAVIOUR 2 Hours / week 5
Credits
1. Study of birds in MCC campus.

2. Orientation of animals (cockroach, ant-lion larvae, maggots, earthworm, grasshopper and
butterfly).
3. Mounting of the legs and sting of honey bees.
4. Study of Drosophila eclosion rhythms.
5. Study of mouth parts in insects and their feeding strategies.
6. Study of learning in mice and cockroach.
7. Establishment of the relation between brain and behaviour.
8. Human actions and gestures
9. Study of animals in captivity – visit to the zoo.
M.Sc. (vi) – SEMESTER IV- MAJOR ELECTIVE : PAPER 19 PRACTICAL V

AQUACULTURE 2 Hours / week 5 Credits
85
1. Identification of cultivable organisms and weed and predatory fishes
i. Fishes (10) b) Shrimps (3) c) Prawns (3) d) Molluscs (5)
2. Identification of aquatic insects (5)
3. Identification of live-feed organisms (field training)
4. Identification / field visit to identify parasites and diseases of fish/shrimp, utility of PCR &
ELISA techniques, medicines and probiotics
5. Water quality analysis and estimation of primary productivity in fish/shrimp farm.
6. Qualitative and quantitative analysis of Planktons.
7. Compulsory visits to 1. Fish farm, 2. Fish Hatchery, 3. Shrimp Farm, 4. Shrimp Hatchery, 5.
Visits to CIBA and CMFRI – Kovalam lab and NIOT)
M.Sc. (vi) SEMESTER IV - PAPER 20 : MAJOR ELECTIVE PRACTICAL - VI

TEACHING ZOOLOGY 3 Hours /week 5
credits
1. Preparation of Teaching aids: a) Wet mount b) Dry mount (insect/crab)
c) skeleton preparation.
2. Preparation of charts and Herbarium sheets
4. Question paper writing – assessment – evaluation.
blue print preparation
5. Lesson plan
6. Power point preparation & presentations
7. Teaching practice in the Zoology Department, MCC.
M.Sc. (vi) SEMESTER IV - PAPER 20 : MAJOR ELECTIVE PRACTICAL - VI

MEDICAL LABORATORY TECHNIQUES 3 Hours /week 5
credits
I.Analysis of Body fluids and Medical Microbiology
Preparation of reagents.
chemical tests – qualitative
Microscopical examination of deposits
Pregnancy tests.
Faeces: routine examination, microscopical examination for ova and parasites. Culture and
sensitivity, sputum for AFB, semen analysis. Collection of sample for culture of microorganisms;
86
different staining techniques, culture and sensitivity test, test for typhoid - Widal / VDRL test,
HIV test, HBSAg (Hepatitis B Surface Antigen) and Mantoux test.
II. Practical Clinical Biochemistry

Determination of the following parameters in blood – Blood sugar, urea, creatinine – cholesterol –
alkaline and acid phosphatase – SGOT and SGPT – total proteins.
III. Haematology and Blood Banking

Collection of blood - Blood cell counts - TC, TRBC – haemoglobin. Haemotological indices,
coagulation tests – ESR tests – peripheral smear study. Blood for malaria and microfilaria – ABO
grouping and Rh.
MADRAS CHRISTIAN COLLEGE (Autonomous)

DEPARTMENT OF ZOOLOGY
M.Phil. CURRICULUM
Papers Title
1. Research Methodology
2. Recent Advances in Zoology
3. Optional: 1. Bioinformatics
2. Reproductive Endocrinology
3. Entomology
87
4. Invertebrate Reproduction
5. Animal Behaviour
6. Fish Biology
88
M.Phil (vi) - SEMESTER I – PAPER-1: MAJOR
RESEARCH METHODOLOGY
1. RESEARCH :
Writing a research paper – style of scientific writing – Bibliography
2. CHROMATOGRAPHY:
Principles and procedures – Unidimensional and two dimensional - paper , thin layer and
gas chromatograph
3. HISTOCHEMISTRY:
Micro techniques, identification of carbohydrates, fats and proteins
4. ELECTROPHORESIS:
Principles – buffers – preparation of materials. Types of electrophoresis and two
dimensional electrophoresis
5. BIOSTATISTICS :
Frequency distribution – class intervals - class limits. General rule for forming frequency
distribution. Histogram – frequency, Relative frequency distribution, Cumulative
frequency distribution, Types of frequency curves. Statistics vs Biology Population and
sample, continuous discontinuous variables, Scientific notation, graphic representation of
data. Mean mode and median – other measures of central tendency Standard deviation and
related measures. Coefficient elements of probability. Tests of hypothesis and significance.
Chi square test, t-test, Regression and correlation ANOVA, Analysis of variance –
parametric and non-parametric.
6. MICROSCOPY AND PHOTOMICROGRAPHY :
Types of microscope – Fluorescent and Electron microscopy – Micrometer – Camera
lucida – elements of photography and microphotography
7. COLORIMETRY AND SPECTROPHOTOMETRY:
General consideration – quantitative estimation of biological compounds in body fluids
and tissues
8. RADIOISOTOPES:
Tracer experiments – Autoradiography – different forms of Counters and laboratory
safety.
Reference Books:
1. Anderson ,J.B.H..,Durston and M.Poole 1970.Thesis and Assignment writing.Wiley

Eastern Pvt.Ltd.,New Delhi.
2. Atkins.H.J.B.,1960.Tools of biological research (Second Series).Blackwell Scientific
Pub.,oxford.
3. Bradshaw,L.J.,1966.Introduction to molecular biological techniques.Prentice Hall Inc.,
New Jersey.Casartelli.,1965.Microscopy for students.McGraw Hill,New Delhi.
4. Hawk,P.B.,B.C.Oset and W.H.Sommerson,1954,Practical Physiological Chemistry.Mc
Graw Hill,New York.
89
5. Homason,G.L.,1967 Animal Tissue Techniques.W.H.Freeman and Company,London.
6. Newman,D.W.(Ed.),1964.Instrumental Methods of Experimental Biology.MacMillan
Co.,New York.
7. Pearse,A.C.E.,1974.Histochemistry,Theoritical and applied .Vol I&II. J.& A .C hurchill
Ltd.,London.Schefler,W.C.,1969.Statistics for the Biological Sciences.
8. Smith,R.C.,1962.Guide to the Literature to the Zoological Sciences.Burgan Publishing
Co.,Minnesota.
9. Whitnay,F.L.,1950.The Elements of Research.
90
M.Phil (vi) - SEMESTER I – PAPER-2: MAJOR
RECENT ADVANCES IN ZOOLOGY
1. Conservation of natural resources

2. Energy of Biological origin and energy Crisis.
3. Population problem and control.
4. Pollution and control measures
5 GENETIC ENGINEERING: techniques and implications
6. HUMAN GENETICS Non-disjunction and its effect – Structural variation and its effects
7. INTEGRATED PEST CONTROL PROGRAMMES for paddy and sugar cane
8. CELL AND TISSUE CULTURE
9. Numerical Taxonomy and principles
10. Biochemical Taxonomy – Trends
11. Aquaculture – Cultivable organisms in India – technology adopted
12. Recent advances in Carcinogenesis
91
M.Phil (vi) - SEMESTER I – OPTIONAL
BIOINFORMATICS
Bioinformatics
Introduction to Bioinformatics. Aims and Tasks. Applications and Research, Information
Retrieval System.
I. Molecular Biology
Introduction. Gene structure Central Dogma hypothesis. Protein structure and Functions.
Recombinant DNA technology. Molecular Biology Techniques: Gel Electrophoresis, DNA
Sequencing. Protein Sequencing.
II. Bioinformatics Tools and Databases
Genomics and Proteomics Sequence Alignment and Analysis. Nucleic acid and Protein
Sequence Databases: Structure Databases. Enzyme, Metabolic Pathway Databases.
Literature Databases Data submission Tools, Data Analysis and Prediction Tools
III Homology, Phylogeny and Evolutionary Trees
Homology and similarity, Phylogeny and relationships, Approaches used in Phylogenetic
analysis, Molecular approaches to Phylogeny, Phylogenetic analysis databases, Hidden
Markov Models for homology modeling.
IV. Microarray Technology
Basic concepts- concept of gene expression. Making Microarray- sample preparation,
hybridization Image acquisition. Prediction of cross hybridization, I m a g e p r o c e s s i n g .
Measuring and quantifying Microarray variability.
V. Drug Discovery and Pharmacoinformatics
Review of basic biological concepts, Characteristics of a drug compound. Discovering a
drug. Target identification and validation. Identifying the lead Compound. Optimization of
Lead compound. Structure based drug design. Molecular docking
Reference Books:
3. Introduction to Bioinformatics 2003
A. Malcolm Campbell & Laurie J. Heyer, Pearson Education ( Singapore) Pvt. Ltd.
92

REPRODUCTIVE ENDOCRINOLOGY
1. Nature of hormones and hormone action

2. Genetic control of synthesis of hormones.
3. Hypothalamus-hypophysial axis and regulatory mechanism
4. Hypophysial and gonadal interrelationship
5. Gonadal development and sexual differentiation
6. Assay of hormones
7. Regulation of fertility
8. Pregnancy and lactation and its impact on endocrine function
9. Disorders of gonadal function
10. Metabolism in relation to reproduction role of adrenal and thyroid glands in reproduction.
93
ENTOMOLOGY
1.PHYSIOLOGY:
Insectan integument –hormones and metamorphosis,insect digestion,circulation
respiration ,excretion,co-ordination and reproduction.
II FORENSIC ENTOMOLOGY:
Scope and Applications.
III.BIOCHEMICAL ANALYSIS:
Involving insect host –plant relationship.
IV.INSECT POPULATION ANALYSIS:
In various habitats .impact of host plants on the reproductive efficiency of the insects. Insect life
tables. Modern control measures. Hazards of insecticides-resurgence and secondary outbreak.
Biology –nature, extent of damage and control of pests of rice, sugarcane, cotton, fruit trees like
mango, orange, banana, grapes-vegetables like brinjal, ladies finger ,etc., groundnut, coconut,-
plantation crops like tea and coffee. Host –Parasite-Predator interactions-dynamics-impact on
population –Biological control.
Reference Books:
1. Fauna of british India Volumes on insects.
2. Geological history and evolution of insects-1953 Scientific American 41,carpenter.
3. Annual Review of entomology Vol 1 to 20
4. Physiology of insect reproduction-Engelmann.
5. Insect endocrinology- K.K.Nayyar.
6. Insect Physiology- Wigglesworth.
7. Introduction to the study of insects- Borror and Delong.
8. Indian Insect Life-Lefroy.
9. Destructive and useful insects- Borror and Delong.
10. Economic entomology-David and Kumarasamy.
11. Insect structure and function-Chapman.
12. General and applied entomology-Nayyar,Ananthakrishnan,David.
13. General Text Book of Entomology-A.D.Imms
14. General Text Book of Entomology – M.S.Mani
15. Ecology of insects-P.W.Price.
16. Annual review of Entomology-1985.
94
INVERTEBRATE REPRODUCTION
1. Introduction to reproductive biology

2. Reproductive organs in invertebrates.
3. Spermatogenesis and oogenesis.
4. Methods of sperm discharge and insemination,fertilization
5. Patterns of reproduction:
Parthenogeneis,asexual reproduction,sexual reproduction,hermaphroditism and sex
reversal.
6. Environmental factors and reproduction
Salanity,humidity,temperature,photoperiod,oxygen and diapauses
7. Endocrine control of production
8. Pheromones and reproduction
9. Invertebrate larvae
10. Metamorphosis
11. Sexual behaviour.
Reference Books:
1. K.K.Nayar, 1977.Invertebrate reproduction.Oxford & IBH Publishing Co.
2. Giese,A.C and Pearse,J.S., 1974.Reproduction of marine Invertebrates
Vols.I.II.III. Academic Press Inc., New York.
3. Wigglesworth,V.B.,1954.The Biochemistry of Decelopment .Pergammon
Press,Oxford.
4. Danileviski,AS.,Photoperiodism and Sexual Development in insects.Oliver and
Boyd,Edinburgh.
5. Counce,S.J.,1972.In Development Systems:Insects.Academic Press,Oxford.
6. Engleman,F.,1970.The Physiology of insect Reproduction.Pergammon
Press,Oxford.
7. Jacobson,M.,1972 Insect Sex Pheremones.Academic Press,New York.
8. Fischer,A and Pfannenstiel,H.D., 1984.Polychaete Reproduction .Gustav
F.Verlag,New York.
9. Dorothy E.Bliss,1983.The Biology of Crustacea.Academic Press.
10. J.M.B.A India .Proceedings of the symposium on Mollusca Part I
11. J.M.B.A India .Proceedings of the symposium on Mollusca Part II.
95
ANIMAL BEHAVIOUR
I.SOCIAL BEHAVIOUR PATTERNS:

1. Means of communication-sound-odour-vision
2. Colonial life –groups-among invertebrates-vertebrates
3. Aggressive behaviour
4. Courtship behaviour
5. Orientation-tropisms-kinesis-taxes-homing
6. Human behaviour –action-gestures.
7. Brain and behaviour
II.RHYTHMIC BEHAVIOURAL PATTERNS:

1. Various categories of rhythms in nature-plant examples-animals-human.
2. Endogenous nature of rhythms-terminology used-zeitgeber-Entrainment and free run
features.
3. Daily and circardian rhythms-their features-significance.
4. Lunar –tidal rhythms-rhythm in marine animals-characteristics-significance.
5. Annual rhythms-protoperioicity-circannual rhythms-migration-hibernation.
6. Biological clocks-biochemical and physiological aspects –significance of endocrine
hormones-location of the clocks.
7. Human rhythm-shift working –time zone transistion-astronautics-applied aspects in
psychiatry,pharmacology,medicine,surgery.
96
FISH BIOLOGY
1. Adaptive radiation in Chondrichthyes and Osteichthyes

2. Morphology and anatomy(all systems)
3. Food and feeding habits
4. Age and growth with special reference to Indian fishes.
5. Spawning and breeding with special reference to Indian fishes.
6. Parasites and diseases.
7. Hill stream fishes.
8. Exotic fishes
9. Larvicidal fishes.
10. Fish in nutrition
11. By –products
12. Taxonomy of commercial important freshwater, estuarine and marine fishes of india.
13. Bionomics of carps ,murrels,pearl spots,mullets,milk fish,sharks and
rays,sardines,mackerels,silver bellies,pomfrets and tunas.
Reference Books:
1.Bagenal,T.B.,1979.The Ageing of fish.Unwin Brothers Ltd.,Greshnam Press,England.
2.Colbert,E.C.,1969.Evolution of the Vertebrates.Wiley Eastern Ltd.New Delhi.
3.Holden,H.T and D.F.S.Rait,1974.Manual of fisheries Science .Part I FAO Technical
Paper No.115.
4.Jhingran,V.G.,1982 Fish and Fisheries of India.Hindustan Publishing Corporation
(India),Delhi.
5.Lagler,K.F.,J.E.,Bordach and R.R.Miller,1962.Ichthyology,the study of fishes. John
Wiley and Sons Inc.,USA.
6.Marshall,N.B.,1965.The Life of Fishes.Wiedenfield and Nicolson. London.
7. Norman,J.R.,1963 .History of Fishes Ernest Benn Ltd.,London.
8. Pillay, T.V.R (Ed.), 1972.Coastal aquaculture in the Indo-Pacific
Region.FAO,Rome,Italy.
9. Qasim, S.Z., 1973.Some Complication of the problem of age and growth in marine
fishes from the Indian Waters. Indian J.Fish.,20(27): 351-371.
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B.Sc.

(vi) –SEMESTER I – Part III a MAJOR

Hickman, Jr.C.P. 1981 Integrated Principles of Zoology 7th Ed.

B.Sc (vi) - SEMESTER I: Part III c. ALLIED I

B.Sc. (vi) - SEMESTER I & II – PART III c : ALLIED - I

B.Sc. (vi) - SEMESTER II – Part III a MAJOR

B.Sc. (vi) - SEMESTER II: Part III a MAJOR

Animal Life - Invertebrata

B.Sc. (vi) - SEMESTER II – PART III c : ALLIED – I

Chordata and General Principles

UNIT II: Pisces

UNIT III: Amphibia

UNIT IV: Reptiles

UNIT VII: Physiology

UNIT VIII: Ecology

UNIT IX: Embryology

B.Sc. (vi) - SEMESTER III – PART III a – MAJOR

Unit:I – Brief History of Cytology (6 classes)

Unit: II – Methods of cell study (12 classes)

Unit: III – Ultrastructure, Biochemistry and functional role of (16 classes)

Unit: IV – Genetic code (12 classes)

Unit :V- Protein Biosynthesis

Unit:VI – Cell Division (10 classes)

2. Cell Biology – DeRobertis et al., VIIth Edition, W.B.Saunders Company, 1985

4. Cell and Molecular Biology- DeRobertis, 8th Ed., Lippincott,2005.

UNIT I : GERM CELL

UNIT III : CLEAVAGE AND GASTRULATION

UNIT IV : EMBRYONIC ADAPTATIONS

UNIT V : PARTHEONOGENESIS AND REGENERATION

UNIT VI : HUMAN DEVELOOPEMENT

UINT VII : REPRODUCTIVE TECHNOLOGY

B.Sc. (vi) - SEMESTER III - PART III a: MAJOR

UNIT I - Endocrine glands

UNIT IV: Pituitary

UNIT VI: Adrenal

UNIT VII: Endocrine functions of the ovary and testis

D. Turner, General Endocrinology.

B.Sc. (vi) - SEMESTER III – PART IIIc: ALLIED II

Ambika Shanmugam 1974. Fundamentals of Biochemistry for Medical Studies. Second

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1. Preparation of solution with ppm, ppt and percentage concentration.

UNIT II : Wildlife ecology

UNIT III: Wildlife in India.

UNIT IV¨ Wildlife management

UNIT VI: Forestry and Sylviculture

UNIT VII: Wildlife conservation and Legislation

B.Sc. (vi) - SEMESTER IV – PART III a : MAJOR

1. Microscopy - dissection and compound microscopes - identifying the different Components.

3. hCG - based pregnancy test.

Unit I: Molecular organization of Cells

Unit II: Gene Technology

Unit III: Enzyme technology

Unit IV: Bioprocess technology

Unit V: Environmental biotechnology

Unit VI: Applications.

B.Sc. (vi) - SEMESTER V – PART III a: MAJOR

Unit-I: Introduction: Scope of Biophysics; Concept and division of Biophysics; Understanding

Unit-II: Energetics: Energy in different forms; laws of thermodynamics; Energy flow in

Unit-III: Molecular Biophysics: Structure of biomolecules – Protein, haemoglobin, myoglobin,

Unit-IV: Methods of studying molecules: Principles of Centrifugation, Spectrophotometer,