Department of Education: Dbes Learning Activity Sheet/Gawaing Pagkatuto
Department of Education: Dbes Learning Activity Sheet/Gawaing Pagkatuto
Department of Education: Dbes Learning Activity Sheet/Gawaing Pagkatuto
Overview
Photosynthesis is the process through which plants use energy from the sun to turn water, carbon dioxide, and
minerals into glucose (food) and oxygen. This process is so important to life on Earth! Not only do animals eat plants, but
they also breathe in the oxygen produced by the plants. When the animals exhale, they provide carbon dioxide in return
that can be used by the plants for more photosynthesis. Humans have also found ways to mimic the natural process of
photosynthesis to create a renewable type of fuel – plant power!
In this exercise, you will observe the photosynthesis process taking place in spinach leaves. You will provide the spinach
with carbon dioxide and light, and observe how the leaves release tiny oxygen bubbles.
https://www.clearwaycommunitysolar.com/blog/science-center-home-experiments-for-kids/learn-how-photosynthesis-
creates-a-natural-chemical-reaction/
Materials
fresh spinach leaves
hole puncher (or can use hard plastic straw)
baking soda (sodium bicarbonate)
liquid dishwashing detergent (only need a drop)
1 plastic syringe (no needle, 10 mL or larger)
2 clear cups
1 measuring cup
1 measuring spoon (1/8 teaspoon)
light source (natural sunlight works, or you can use an artificial light)
Procedure
1. Use a measuring spoon to scoop out 1/8 teaspoon of baking soda, and fill your measuring cup with 300
milliliters of water. Mix the baking soda into the water to create your bicarbonate solution. When the
baking soda dissolves in the water, it releases carbon dioxide. This will be used by the spinach for
photosynthesis. Pour this solution into any cup and set aside.
2. Fill your clean measuring cup with 200 milliliters of water and add a drop of liquid dishwashing
detergent to create a detergent solution.
3. Pour half (150mL) of your bicarbonate solution into a clear cup. Place a drop of the detergent solution
into the bicarbonate solution. Make sure there is no suds. If you see bubbles, continue adding small
amounts of the bicarbonate solution to the cup until the bubbles are gone.
4. Using the hole punch, create 10 – 20 discs from the spinach leaves. Only punch holes in smooth, flat
areas and do your best to avoid large veins or the edges of the leaf.
5. Remove the plunger from the syringe and add the discs into the syringe.
6. Put the plunger back into the syringe and slowly depress it to expel as much air as you can without
crushing the leaves.
7. Dip the tip of the syringe in the bicarbonate/detergent mixture and pull in about 3 mL of liquid. Gently
tap the syringe to suspend the leaves in the solution.
8. Next, place your finger over the end of the syringe and pull back on the plunger to create a vacuum.
9. While keeping your finger over the tip to maintain the vacuum, swirl the leaf disks in the syringe. After
10 seconds, remove your finger to release the vacuum.
10. Repeat steps 8 and 9 to make sure the leaves take up the carbon dioxide. You should observe the discs
sinking to the bottom of the syringe. If this does not happen, you may need to start over using fresh discs
and new solution that has more baking soda.
11. Pour the discs into the cup with the bicarbonate/detergent mixture and watch them sink to the bottom.
Make sure they don’t stick to the sides of the cup.
12. Expose the cup to natural sunlight or artificial light source for several minutes. Observe and write down
what happens as the spinach discs perform photosynthesis and give off oxygen.
Observations:
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Follow up questions
1. What happened to the spinach discs as they gave off oxygen from photosynthesis?
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2. What happens to the spinach discs when you turn off the light source?
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Conclusion:
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“Obstacles are those frightful things we see when we take our eyes off our
goal”
- Henry Ford
S.Y 2022-2023