This document is an assignment for the course BT3040. It discusses using various tools on the EMBOSS online platform to analyze a DNA sequence, including finding the complementary strand, translating a DNA sequence to a protein sequence, determining the reading frame of a protein sequence, and calculating the GC content and melting temperature of sequences. It also discusses using the backtranseq and geecee functions on EMBOSS.
This document is an assignment for the course BT3040. It discusses using various tools on the EMBOSS online platform to analyze a DNA sequence, including finding the complementary strand, translating a DNA sequence to a protein sequence, determining the reading frame of a protein sequence, and calculating the GC content and melting temperature of sequences. It also discusses using the backtranseq and geecee functions on EMBOSS.
This document is an assignment for the course BT3040. It discusses using various tools on the EMBOSS online platform to analyze a DNA sequence, including finding the complementary strand, translating a DNA sequence to a protein sequence, determining the reading frame of a protein sequence, and calculating the GC content and melting temperature of sequences. It also discusses using the backtranseq and geecee functions on EMBOSS.
This document is an assignment for the course BT3040. It discusses using various tools on the EMBOSS online platform to analyze a DNA sequence, including finding the complementary strand, translating a DNA sequence to a protein sequence, determining the reading frame of a protein sequence, and calculating the GC content and melting temperature of sequences. It also discusses using the backtranseq and geecee functions on EMBOSS.
1. Since I’m a Mac user, I have used the online tool given in the link (https://www.bioinformatics.nl/cgi-bin/emboss). 2. The complementary strand for the given DNA sequence can be found by the following steps:
• Open EMBOSS via the link.
• Search for the revseq function on the left-hand side of the window and click on it.
• Copy and paste the DNA sequence into the space where the DNA sequence can be entered manually.
4. (i) To ind the protein sequence, use the function transeq in EMBOSS.
(ii) From the above image, it is clear that the reading frame equivalent to the given protein sequence is 4th one (the irst reverse reading frame).
3 f f
7. Backtranseq function
• It can be used to get the DNA sequence from a given protein
sequence. It gives the nucleic acid sequence it is most likely to have come from.
• For each amino acid in the input sequence, the corresponding most frequently occurring codon is used in the output nucleic acid sequence.
Geecee function
• Geecee gives the fraction of G+C bases of the entered nucleic acid sequence(s).
• It basically sums the number of G and C bases in the sequence(s) and
writes the result as the fraction in the interval 0.0 to 1.0.
8. (i)
(ii)
We can see that the melting temperature of the irst sequence with only A and T is 48 degrees and the melting temperature of the second sequence is 108 degrees. This indicates the as the GC content of the sequence increases, the melting temperature as well as the stability also increases.
9. The AT_content of the given sequence is 58.333333% and GC_content is 41.666667%.
