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    2023-03-24 Printed As PDF Presi Pt04

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    sauronsauron
    The document describes a selection scheme for a desired chemical reaction. The scheme uses modified DNA (fDNA) that undergoes the desired reaction and is then separated from discarded fDNA using affinity chromatography. The fDNA is then photocleaved and the fragments amplified by PCR to enrich for DNA that underwent the desired reaction.

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    2023-03-24 Printed As PDF Presi Pt04

    Uploaded by

    sauronsauron
    11 views2 pages
    The document describes a selection scheme for a desired chemical reaction. The scheme uses modified DNA (fDNA) that undergoes the desired reaction and is then separated from discarded fDNA using affinity chromatography. The fDNA is then photocleaved and the fragments amplified by PCR to enrich for DNA that underwent the desired reaction.

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    2023-03-24_Printed_as_PDF_Presi_Pt04

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    The document describes a selection scheme for a desired chemical reaction. The scheme uses modified DNA (fDNA) that undergoes the desired reaction and is then separated from discarded fDNA using affinity chromatography. The fDNA is then photocleaved and the fragments amplified by PCR to enrich for DNA that underwent the desired reaction.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    11 views2 pages

    2023-03-24 Printed As PDF Presi Pt04

    Uploaded by

    sauronsauron
    The document describes a selection scheme for a desired chemical reaction. The scheme uses modified DNA (fDNA) that undergoes the desired reaction and is then separated from discarded fDNA using affinity chromatography. The fDNA is then photocleaved and the fragments amplified by PCR to enrich for DNA that underwent the desired reaction.

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    © All Rights Reserved
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    Desired Reaction:

    NH2
    N N

    O N N
    O -O P O O
    O-
    HN NH
    O O OH
    H H O
    H
    N
    S N R
    H
    O
    O O
    O P O 5´ fDNA 3´
    NH2 H N
    H O-
    N O
    O NO2

    HN NH
    H H O R O NO2
    H H
    N N
    S N N O
    H H H O-
    O O N
    O P O 5´ fDNA 3´
    O O
    Selection Scheme II
    5´ 3´ Discarded fDNA
    A
    A
    Desired reaction B

    BA Selection by affinity-
    chromatography
    A
    h·

    BA

    Photocleavage

    B A

    A
    5´ 3´
    Amplification (PCR)
    Primer extention with natural dNTP´s
    with modified dNTP`s

    Regular DNA
    Mod. DNA (fDNA)

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