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    Estimation of Blood Glucose

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    podcast gazal
    The document describes an experiment to estimate blood glucose levels using the Orthotoluidine method. This method involves reacting glucose with o-toluidine in glacial acetic acid and heating to produce a blue-green derivative whose intensity is proportional to glucose concentration. Three test tubes are set up with different mixtures of sample, standard, and water. After mixing and heating, the optical densities are measured and glucose concentration is calculated using the standard values. Normal glucose levels and the meaning of hyperglycemia and hypoglycemia are also defined.

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    Estimation of Blood Glucose

    Uploaded by

    podcast gazal
    139 views3 pages
    The document describes an experiment to estimate blood glucose levels using the Orthotoluidine method. This method involves reacting glucose with o-toluidine in glacial acetic acid and heating to produce a blue-green derivative whose intensity is proportional to glucose concentration. Three test tubes are set up with different mixtures of sample, standard, and water. After mixing and heating, the optical densities are measured and glucose concentration is calculated using the standard values. Normal glucose levels and the meaning of hyperglycemia and hypoglycemia are also defined.

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    ESTIMATION OF BLOOD GLUCOSE

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    The document describes an experiment to estimate blood glucose levels using the Orthotoluidine method. This method involves reacting glucose with o-toluidine in glacial acetic acid and heating to produce a blue-green derivative whose intensity is proportional to glucose concentration. Three test tubes are set up with different mixtures of sample, standard, and water. After mixing and heating, the optical densities are measured and glucose concentration is calculated using the standard values. Normal glucose levels and the meaning of hyperglycemia and hypoglycemia are also defined.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    139 views3 pages

    Estimation of Blood Glucose

    Uploaded by

    podcast gazal
    The document describes an experiment to estimate blood glucose levels using the Orthotoluidine method. This method involves reacting glucose with o-toluidine in glacial acetic acid and heating to produce a blue-green derivative whose intensity is proportional to glucose concentration. Three test tubes are set up with different mixtures of sample, standard, and water. After mixing and heating, the optical densities are measured and glucose concentration is calculated using the standard values. Normal glucose levels and the meaning of hyperglycemia and hypoglycemia are also defined.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 3

    EXPERIMENT No.

    AIM: To estimate the blood glucose level by Orthotoluidine method

    Principle: Glucose reacts with o-toluidine in glacial acetic acid on heating to yield a blue-green
    N-glycosylamine derivative. The intensity of this colour is proportional to the concentration of
    glucose present.

    Procedure: Take three test tube mark it T (Test), S (Standard) , B(Blank).

    Reagents Blank Standard Test

    Sample - - 0.1ml

    Standard - 0.1ml -

    Distilled water 0.1ml - -

    O- Toluidine reagent 5ml 5ml 5ml

    Mix the contents of each test tube and keep in a boiling water bath for 10 minutes.

    Cool and take the OD on colorimeter reading at 625 nm.

    OBSERVATION:

     OD of blank -
     OD of standard -

     OD of test –

    Calculation:

    Concentration of glucose in mg/100 ml blood.

    = ODT - ODB / ODS - OD B * 100mg%

    Result: The given sample contains …….mg/dl of blood sugar.

    Interpretation:

    In normal healthy persons

    Fasting plasma glucose is 70-110 mg/dl

    Post parandial glucose is less than 140 mg/dl


    When blood glucose concentration is within the normal range, it is referred to as
    normoglycemia.

    When values are higher than the normal range, it is known as hyperglycemia.

    When the values are lower than the normal limits, it is called as hypoglycemia.

    FBS > 140 mg/dl Indicative of diabetes mellitus

    PPBS > 200mg/dl

    Hyperglycemia is observed in

     Diabetes mellitus
     Hyper function of the pituitary and adrenal glands (acromegaly, Cushing syndrome,
    pheochromocytoma, etc)
     Emotional states like fear, anger and anxiety lead to elevation in blood sugar levels
    because of secretion of epinephrine which has a hyperglycemic action
     Meningitis, encephalitis, shock
     Severe hemorrhage
     Nephrotic syndrome, brain tumour and sepsis

    Hypoglycemia is seen in

     Insulin overdose in patients of diabetes


     Pancreatic tumours affecting beta cells
     Severe liver disease
     Von Gierke’s disease
     Functional or reactive hypoglycemia
    VARIOUS METHODS OF ESTIMATION OF BLOOD GLUCOSE

    NON ENZYMATIC METHOD ENZYMATIC METHOD

    1. Nelson – Somogyi method Hexokinase method

    2. Folin Wu method Glucose oxidase – peroxidase


    method

    3. Asatoor king method Glucose dehydrogenase method

    4. O – toluidine method

    5. Benedict’s/ Fehlings test

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