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    Sarmentosamide Group4

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    Oscar Angelo Jr.
    This document summarizes research on a compound called sarmentosamide extracted from the marine-derived Streptomyces sp. APmarine042 bacteria. Through nuclear magnetic resonance analyses and bioactivity-guided isolation, the researchers determined that sarmentosamide has a unique chemical structure consisting of a hexadienamide moiety and two rare structural motifs. Cell culture experiments showed that sarmentosamide suppresses MMP-1 expression and has anti-aging properties. The researchers concluded that sarmentosamide is a novel compound with potential anti-aging applications.

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    Sarmentosamide Group4

    Uploaded by

    Oscar Angelo Jr.
    21 views14 pages
    This document summarizes research on a compound called sarmentosamide extracted from the marine-derived Streptomyces sp. APmarine042 bacteria. Through nuclear magnetic resonance analyses and bioactivity-guided isolation, the researchers determined that sarmentosamide has a unique chemical structure consisting of a hexadienamide moiety and two rare structural motifs. Cell culture experiments showed that sarmentosamide suppresses MMP-1 expression and has anti-aging properties. The researchers concluded that sarmentosamide is a novel compound with potential anti-aging applications.

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    SARMENTOSOMIDE

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    Sarmentosamide_group4

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    This document summarizes research on a compound called sarmentosamide extracted from the marine-derived Streptomyces sp. APmarine042 bacteria. Through nuclear magnetic resonance analyses and bioactivity-guided isolation, the researchers determined that sarmentosamide has a unique chemical structure consisting of a hexadienamide moiety and two rare structural motifs. Cell culture experiments showed that sarmentosamide suppresses MMP-1 expression and has anti-aging properties. The researchers concluded that sarmentosamide is a novel compound with potential anti-aging applications.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    21 views14 pages

    Sarmentosamide Group4

    Uploaded by

    Oscar Angelo Jr.
    This document summarizes research on a compound called sarmentosamide extracted from the marine-derived Streptomyces sp. APmarine042 bacteria. Through nuclear magnetic resonance analyses and bioactivity-guided isolation, the researchers determined that sarmentosamide has a unique chemical structure consisting of a hexadienamide moiety and two rare structural motifs. Cell culture experiments showed that sarmentosamide suppresses MMP-1 expression and has anti-aging properties. The researchers concluded that sarmentosamide is a novel compound with potential anti-aging applications.

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    © All Rights Reserved
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    Download as pdf or txt
    of 14

    SARMENTOSAMIDE AN ANTI-AGING

    COMPOUND FROM MARINE DERIVED


    STREPTOMYCES SP. APMARINE042
    GROUP 4 - BS BIOLOGY 4101

    MAGDALES, EUGENIO
    PANOTES, HAVEN ANTONY
    TAPALES, LORENZ ALBERT
    CASTILLO, KARLA CAMILLE
    MACABIDANG, LIANNA MARIE
    MARIN, GILLIAN
    1. INTRODUCTION

    2. EXTRACTION

    3. ISOLATION
    Table of Contents
    4. STRUCTURE ELUCIDATION

    5. CHARACTERIZATION

    6. APPLICATION / USES
    SARMENTOSAMIDE
    WHAT IS THE BIOMOLECULE?

    STREPTOMYCES SP. APMARINE042


    NUCLEAR MAGNETIC RESONANCE (NMR) ANALYSES
    SARMENTOSAMIDE
    HEXADIENAMIDE MOIETY.

    TWO RARE STRUCTURAL MOTIFS – THE 2Z,4E-UNSATURATED


    ACYL CHAIN AND THE NITROGEN-CONTAINING RIGHT-HALF
    PORTION
    Compound from
    Streptomyces sp. APmarine042

    HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH


    INCORPORATION OF ULTRAVIOLET SPECTROSCOPY (HPLC-
    UV) AND BIOACTIVITY-GUIDED ISOLATION
    STRUCTURE ELUCIDATION
    SARMENTOSAMIDE CHEMICAL STRUCTURE

    a. ELUCIDATION OF SARMENTOSAMIDE

    SARMENTOSAMIDE IS PRODUCED UNDER THE SAME


    CULTIVATION CONDITIONS WITH TWO KNOWN AND
    STRUCTURALLY UNRELATED BIOACTIVE COMPOUNDS ---
    SPECTINABILIN AND TRYPTOPHAN-DEHYDROBUTYRINE
    DIKETOPIPERAZINE
    STRUCTURE ELUCIDATION
    SARMENTOSAMIDE CHEMICAL STRUCTURE

    a. ELUCIDATION OF SARMENTOSAMIDE
    THE PLANAR STRUCTURE OF SARMENTOSAMIDE WAS
    ELUCIDATED AS N-(40 -CARBAMOYL-30 E-PENTEN-20 -
    YL)HEXA-2Z,4E-DIENAMIDE
    CHARACTERIZATION
    CHEMICAL AND BIOLOGICAL ASSAY

    a. CELL CULTURE AND VIABILITY ASSAY


    NORMAL HUMAN DERMAL FIBROBLASTS-NEONATAL
    (NHDFS) AND HUMAN FORESKIN FIBROBLAST CELL LINE
    (HS68)
    DULBECCO’S MODIFIED EAGLE’S MEDIUM (DMEM)
    PRIMARY FIBROBLAST, SMOOTH MUSCLE AND NEURONS
    PC-12 AND HELA
    10% FETAL BOVINE SERUM (FBS)
    100 U/ML POTASSIUM PENICILLIN
    100 MG/ML STREPTOMYCIN SULFATE
    37°C; HUMIDIFIED 5% CO INCUBATOR
    CCK-8 REAGENT
    CHARACTERIZATION
    CHEMICAL AND BIOLOGICAL ASSAY

    b. MMP-1 ELISA
    (Enzyme-Linked Immunosorbent Assay)
    SERUM-FREE DMEM MEDIA
    18 HOURS
    25 MJ/CM2 UVB
    20 NG/ML OF TNFA
    SARMENTOSAMIDE (2.5, 5, 10 UG/ML)
    MMP-1 ELISA KIT
    BCA PROTEIN
    CHARACTERIZATION
    CHEMICAL AND BIOLOGICAL ASSAY

    c. Western Blot
    ALIQUOTS OF THE CELL LYSTATES
    MITOGEN-ACTIVATED PROTEIN KINASE (MAPK)
    TRIS-BUFFERED SALINE (TBS)
    5% BOVINE SERUM ALBUMIN (BSA)
    0.1% TWEEN-20; 4°C
    ROOM TEMPERATURE; 1 HOUR
    PEROXIDASE-CONJUGATED SECONDARY ANTIBODIES
    5% BSA
    0.1% TWEEN-20
    ENHANCE CHEMILUMINESCENCE (ECL) SUBSTRATE
    CHEMILUMINESCENT SIGNALS
    CHARACTERIZATION
    CHEMICAL AND BIOLOGICAL ASSAY

    d. DPPH Radical Scavenging Assay


    2,2-DIPHENYL-1-PICRYLHYDRAZYL (DPPH)
    100% ETHANOL-BASED DPPH SOLUTION
    190 UL DPPH SOLUTION
    10 UL OF THE VARIOUS DOSES OF SARMENTOSAMIDE
    30 MINUTES; 37°C
    ABSORBANCES: 517 NM
    APPLICATION AND USES
    SARMENTOSAMIDE

    Anti-aging properties
    SUPPRESSION OF MMP-1
    REFERENCES
    EUN-SON L. ET. AL (2020). SARMENTOSAMIDE, AN ANTI-
    AGING COMPOUND FROM A MARINE-DERIVED
    STREPTOMYCES SP. APMARINE042. RETRIEVED FROM
    HTTPS://WWW.NCBI.NLM.NIH.GOV/PMC/ARTICLES/PMC75517
    00/?FBCLID=IWAR0-
    9P7YUCERXSYWFTKLOXKOXPEL8NL0K9XX2YTL_G9LG_UZUQ
    8OFYNCBK4.
    KITANI, S. ET. AL (2013). SARMENTOSAMIDE, A NOVEL
    HEXADIENAMIDE FROM THAI SOIL ACTINOMYCETES.
    RETRIEVED FROM HTTPS://SCI-
    HUB.SE/10.1080/14786419.2012.671313?
    FBCLID=IWAR2W2BGGFGTCYKL7AFGWELX0GZP1S4QA5IUVK
    1RUQKNMT1ZMQASGIJGX_PK.
    THANK YOU

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