Enzymes
Enzymes
Enzymes
I. Objectives
In conducting this experiment, the following objectives are followed:
1. To observe the breakdown of hydrogen peroxide toxin by potato's enzyme
catalase.
2. To determine if temperature and pH affect enzyme effectiveness.
II. Procedures
1. Prepare the potato wedges.
1.1 Potato 1 – normal 2 cm size.
1.2 Potato 2 – normal 2 cm size but boiled.
1.3 Potato 3 – small bits of potato.
1.4 Potato 4 – normal 2 cm size.
4. Look at the bubbles produced, which shows the oxygen produced from the
chemical reaction
III. Experimental Setup and Materials
Before
After
Materials:
Picture/Image Name Function
Marker Marker – used to mark the potato in order
Ruler to cut it properly.
Chopping Ruler – used to measure the potato.
Board Chopping Board – used to protect the
Knife surface of the table and to ensure that the
potato is cut properly.
Knife – used to precisely cut the potato.
Test Tubes
Test Tubes – used to be the place where
the chemical reaction takes place.
Test Tube Test Tube Rack – used to hold the test
Rack tubes in place.
V. Analysis
Test Tube 1 Test Tube 2 Test Tube 3 Test Tube 4
3. Add 2 cubic cm of urease solution to each test tube and shake well.
After
Materials:
Picture/Image Name Function
Pipette Dropper Used to collect and
drop the chemicals
being used.
Urea Solution Used to test as an
inhibitor
Bromothymol Blue Used as an
Indicator indicator
Mercuric Chloride Used to test as an
Solution inhibitor
Distilled Water Used to test as an
inhibitor
3. Most proteins become inactive once they are coupled to mercury. In its most basic
form, mercury binds irreversibly to an enzyme, altering its conformation and
inhibiting the binding of its typical substrate.
4. A. Competitive inhibition - When molecules that are very identical to the substrate
molecules bind to the active site, they hinder the actual substrate from binding.
B. Mixed type inhibition - When the inhibitor attaches to the enzyme in a different
position than the one where the substrate will bind, this is known as mixed
inhibition.
C. Non-competitive inhibition - It is a particular kind of enzyme inhibition where an
inhibitor binds to an allosteric location and reduces the enzyme's effectiveness.
5. When hydrogen peroxide had a contact with its substrate, enzyme catalase, it starts
to decompose into water and oxygen.
References (for both)
education.com. (2013, November 18). Catalase and Hydrogen Peroxide Experiment.
https://www.education.com/science-fair/article/activator/
v=3PYdMaClUmw