HPLC LQ

Index HPLC Chromatography
HPLC Chromatography
Content Page Content Page
USP Column Listing 186-187 Fingertight Fittings - High Pressure Fittings 242
Hardware Design Column: Ultrafit™ System 188 Fingertight Fittings - Stainless Steel Tubing 243
Novacol™ Columns 189
Microbore Columns 190 PEEK™ tubing 244
Ultrarapid Columns 191
Preparative Columns 192 Inlet Solvent Filters 245
Guard Columns for HPLC 193-196 Bottle Caps - PEEK Bottom-of-the-Bottle Solvent Filters 246
Precolumn and Inline Filters 247
Mediterranea™ Sea18 HPLC Column 197-208
Deuterium Lamps for a Detector 248-251
Technical Information 197-204
Mediterranea™ Producucts List 205-2067 HPLC Safety Caps 252-255
Mediterranea™ Sea18 New UHPLC Columns 208 Exhaust Filters for Safety Caps 255
HPLC Safety Waste Caps 256
Brisa LC2 HPLC Column 209 HPLC Safety Caps - With Safety Funnel 256
Europa™ HPLC Column for Peptides Sample Injection Rheodyne™ Valves 257-260
and Proteins 210-216 Rheodyne Injecion Valves for Microscale Applications 257
Technical Information 210-212 Rheodyne Injection Valves for Preparative Applications 258
Europa™ Products List 213-216 Rheodyne RheBuild™ Kits 259
Sampling Loops for Rheodyne Injection Valves 259
Tracer Excel™ 217-227 PEEK Sampling Loops 260
Tracer Excel ODS-B 219-220 Rheodyne Accessories 260
Other Tracer Excel Packings 221
General Properties of Tracer Excel Packings 222
Tracer Excel 120 223-226
Tracer Excel 300 227
Tracer Extrasil 228-233
Hyperpack ODS 234-235
Hyperpack Basic 236-237
Lichrosorb 238
Lichrospher 239
Tracer Carbohydrates 240
Tracer Excel PFP 241
Tracer Excel PAH 241
185
USP Column Listing
Octadecyl silane chemically bonded to porous silica or ceramic microparticles, 3 to 10 µm in diameter. MEDITERRANEA SEA18
L1 TRACER EXCEL 120 ODS A
TRACER EXCEL 120 ODS B
Brisa LC2 C18
TRACER EXTRASIL ODS2
TRACER EXTRASIL ODS1
Advantix ODS
Hyperpack ODS
Hyperpack BASIC
TSKgel ODS
YMC PRO C18
HYPERSIL
HyPURITY C18
LICHROSORB RP18
LICHROSPHER RP18
NUCLEOSIL 100 C18
NUCLEOSIL 120 C18
PARTISIL ODS3
Porous silica microparticles, 5 to 10 µm in diameter. TRACER EXCEL 120 Si
L3 TRACER EXTRASIL Si
PINNACLE Si
HYPERSIL Si
ULTRA Si
PINNACLE Si
LICHROSORB Si
LICHROSPHER Si
NUCLEOSIL 100 Si
NUCLEOSIL 120 Si
PARTISIL Si
Octyl silane chemically bonded to totally porous microsilica particles, 5 to 10 µm in diamerer. TRACER EXCEL 120 C8
L7 TRACER EXTRASIL C8
Advantix C8
ULTRA C8
PINNACLE C8
TSKgel oCTYL
HYPERSIL C8
LICHROSORB RP8
LICHROSPHER RP8
NUCLEOSIL 100 C8
NUCLEOSIL 120 C8
An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally porous silica gel TRACER EXCEL 120 APS
L8 support, 10 µm in diameter. TRACER EXTRASIL NH2
TRACER EXCEL 120 C8
HYPERSIL NH2
LICHROSORB NH2
LICHROSPHER NH2
NUCLEOSIL 100 NH2
NUCLEOSIL 120 NH2
TRACER EXTRASIL SCX
L9 PARTISIL SCX
Nitrile groups chemically bonded to porous silica microparticles, 5 to 10 µm in diameter. TRACER EXCEL 120 CN
L10 TRACER EXTRASIL CN
HYPERSIL CPS
HYPERSIL BDS CPS
LICHROSORB CN
LICHROSPHER CN
NUCLEOSIL 100 CN
NUCLEOSIL 120 CN
Phenyl groups chemically bonded to porous silica microparticles, 5 to 10 µm in diameter. TRACER EXCEL 120 PHENYL
L11 TRACER EXTRASIL PHENYL
NUCLEOSIL 100 P
Trimethylsilane chemically bonded to porous silica microparticles, 5 to 10 µm in diameter. TRACER EXCEL 120 C1
L13 TRACER EXTRASIL C1
186
USP Column Listing
Silica gel, 10 µm in diameter, having a chemically bonded, strongly basic quaternary ammonium anion- TRACER EXTRASIL SAX
L14 exchange coating.
Hexyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter. TRACER EXTRASIL C6
L15
Dimethyl silane chemically bonded to totally porous silica particles, 5 to 10 µm in diameter. NUCLEOSIL 100 C2
L16
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in HAMILTON HC-75
L17 the hydrogen form, 7 to 11 µm in diameter. HYDROGEN FORM
COREGEL 87H
ORH-801
ION-300
Amino and cyano groups chemically bonded to porous silica particles, 5 to 10 µm in diameter. PARTISIL PAC
L18
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in CARBOSEP CHO-820
L19 the calcium form, 9 µm in diameter. CARBOSEP CHO-620
COREGEL 87-C
CARBOSEP USP L19 CA
HAMILTON HC-75 CALCIUM
Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm in diameter. LICHROSORB DIOL
L20 LICHROSPHER DIOL
A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter. HAMILTON PRP-1

L21
A cation exchange resin made of porous polystyrene gel wifh sulfonic acid groups, about 10 µm in HAMILTON PRP-X200
L22 diameter.
An ion exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium HAMILTON PRP-X500
L23 groups, about 10 µm in size.
A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix TOYOPEARL HW, F Grade
L24 surface, 32 to 63µm in diameter.
Packing having the capacity to separate compounds with a MW range from 100 to 5000 daltons (as TSK-GEL G2500PW
L25 determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A TSK-GEL G2500PWXL
polymethacrylate resin base, crosslinked with polyhydroxylated ether (surface contained some residual TSK-GEL G-Oligo PW
carboxyl functional groups) was found suitable.
Butyl silane chemically bonded to totally porous silica particles, 5 to 10 µm in diameter. TRACER EXCEL 120 C4
L26
Porous silica particles, 30 to 50 µm in diameter. Ymc-PACK SILICA 30/60
L27
Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10 µm in diameter. LICHROSORB RP-2
L30
Packing having the capacity to separate proteins of 4000 to 400000 daltons. It is spherical, silica-based TSK GEL SW AND SWXL
L33 and processed to provide pH stability. SERIES
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in CARBOSEP CHO-682
L34 the lead form, about 9 µm in diameter. HAMILTON HC-75 Pb
Polymethacrylate gel packing having the capacity to separate proteins by molecular size over a range of TSK-Gel G 3000 PWXL
L37 2,000–40,000Da MW
Methacrylate-based size exclusion packing for water-solubles TSK-GEL PW/PWXL
L38
Cellulose tris-3,5-dimethylphenylcarb-amate coated porous silica particles, 5 to 20 µm in diameter CHIRALCEL AD
L40
Immobilized alpha-acid glyco-protein on spherical silica particles, 5 µm in diameter CHIRAL-AGP
L41
Pentafluoropehnyl groups chemically bonded to silica particles 5 to 10 µm in diameter Hypersil GOLD PFP
L43
187
Hardware Design Column: Ultrafit™ System
New Hardware Design Column: Ultrafit™ System Our Columns Mediterranea™, Europa Peptides,
Europa Proteins & Tracer Excel are built with the
The new Ultrafit™ design will make your work in the laboratory more
comfortable and efficient. The Ultrafit™ system, as well as helping new Ultrafit ™ System
in the replacement of the frit at the column entrance, enables you Peaks:
to easily include either additional frits or a pre-column, always with 2 1) Uracil (TM)
the utmost simplicity and economy and in no way whatsoever is the 2) Naphthalene
3) Acenaphthene
quality of the separation affected. 4) Anthracene
In designing the Ultrafit™ column, the greatest care has been taken 3
to cover all the aspects that may occur in the loss of efficiency of 4
the column. As a result of all this, dead volumes have been reduced
to a minimum, entered by the system by means of a high precision 1
mechanism, with inlet and outlet holes of 0.2 mm and first-class

tapers for the perfect distribution of the inlet and outlet flows, as 0 2 4 6
seen in the three depicted Ultrafit™ options. The Ultrafit™ system Minutes
enables a pre-column to be included without loss of efficiency, to Column with Ultrafit™ System
columns as small as 30 x 4 mm packed with particles of 3 mm. 2
Moreover, the very best material has been selected for the
construction of the column, with an ultra-shiny interior finish, of
extremely low RMS, ensuring that no tube imperfection in the
column will affect the quality of the separation. 3
4
Ultrafit™ System Efficiency

1
Efficiency AS
Column (N/m) (10%)
mediterranea sea18 Column 3 µm 134904 1,11 0 2 4 6

5 x 0,46 cm Ultrafit™ System Minutes
mediterranea sea18 Column 3 µm 135042 1,05 Column with Ultrafit™ System + Ultrafilter™
5 x 0,46 cm with Prefilter Ultrafilter™
2
mediterranea sea18 Column 3 µm 137819 1,07
5 x 0,46 cm with Precolumn Ultraguard™
Chromatographic Conditions:
Column: mediterranea sea18 3 mm 5 x 0,46 cm 3
Eluant: Acetonitryle/Water 65:35 4
Flow: 0,9 ml/min
Det. UV 254 nm 1
Temp. Room
Sample: Acenaphthene 0,2 mg/ml
0 2 4 6
Minutes
Column with Ultrafit™ System + Ultraguard™

Ultrafit™ System Configuration
Ultrafit™
Ultrafit™ + Ultrafiter™
UltrafitT™ + Ultraguard™
188
Novacol™ Columns
To get HPLC columns with maximum efficiency and peak symmetry,
Teknokroma uses tubing and connections designed and fully
optimized to provide you superior performance than achievable
with columns from the major manufacturers.
The Novacol™ columns, designed and manufactured by

Teknokroma, use the best bonding reagents, packing support
materials and proprietary Novabond™ procedures. Novacol™
tubing uniformity and polished interior finish generates higher
efficiencies than columns from the major manufacturers. The latest
in current research trends in HPLC are included in Novacol™
columns; including smaller particle size, greater particle uniformity,
reduced tubing internal diameters and shorter columns for LC-
MS applications. Novacol™ columns are designed with a new
generation of tubing interior surfaces, connections,
end-fittings and packing procedures. Our Novabond™ proprietary
procedures allows us to manufacture columns as small as 2mm ID
with 3 µm particles and columns as short as 5cm long with 2mm ID
with no loss in theoretical efficiency.
Our Novacol™ columns have added another new feature - the

incorporation of Microtaper™ in the design and manufacture of our
Novacol frits to optimize the correct sample filtering distribution at
the entry and exit of the column.
Lastly, we designed Novacol™ columns to allow you to easily

change frits without running the risk of affecting the column packing
during the exchange. Novacol™ columns are compatible with all
10/32 Valco-type connections.
Novacol™ columns are available in a wide range of standard

internal diameters (4.6, 4.0, 3.0, and 2.1mm ID) and various
standard lengths (3, 5, 10, 12.5, 15, 20, 25, and 30cm), which
allows you to adapt to all chromatographic modes: microbore,
ultrafast and analytical.
189
Microbore Columns
Microbore Columns
Low Dispersion Chromatography
Our experience in the manufacture of HPLC columns allow us to
offer the possibility to work with this interesting chromatographic
concept. These columns of 2 and 3mm of internal diameter, packed
with the same packings than 3 and 5 µm analytical columns,
Eluyent waste (ml/8 hours)

contribute to an important solvents saving and at the time a
detectability considerable increase.
Detectability
Sensibility of Detection
Since the detectability depends on the grade of dilution of the
sample while it passes through the column, a reduction of the
internal diameter of the column redounds directly in a minor dilution
and therefore in an increment of the detection sensibility.
Solvents Saving
The same chromatogram obtained with a conventional 4,6 mm ID
Column Diameter
column working at 2 ml/min can be obtained with a flow of 0,4 ml/
min when it is worked with a 2,1 mm ID microbore column. This Eluyent waste (ml/8 hours)
represents a 80 % saving of the eluyent wasted in HPLC, which Detectability
means that for a standard job in a chromatograph will represent a

saving of 15 liters of solvent.
Instrumentation
The level of development achieved by the instrumentation of
HPLC allows that these kind of columns can be used by most of the
commercialized chromatographs.
In many cases, the 90 % of efficiency loss owed to the
chromatograph system, can be eliminated simply with the
optimization of connections and the capilar tubes that connect the
injector to column and column to detector.
Column (mm) Eluyent Waste Detectability

4.6 480 1
4.0 363 1.322
3.2 232 2.066
2.1 100 4.798
1 22.68 21.16
Available also 1 mm internal diameter columns.

Please contact with your representative.
190
Ultrarapid Columns
High-speed chromatography Economy
The use of ultrarapid columns is ideal when short times of analyses The reduced time of analysis that is achieved with these columns
are needed (0.5-3.0 min) and high efficiencies of separation. These and therefore the elevated number of samples that can be
columns 3-10 cm of length, are packed with spherical packs of 3 processed per time unit, compared with conventional columns,
µm, and offer efficiencies of 5-15000 N column (equivalents to 120- allows optimizing to the full the performance of one chromatographic
150000 N/m ), more than enough for the majority of separations. equipment. The extensive selection of available phases allows
turning any chromatographic separation into ultrarapid, with all the
Sensibility of detection advantages that this bears.
Reducing the size of particle the dispersion of the sample in the
inside of the column decreases also. Instrumentation
In this way, the use of ultrarapid columns give a significant The use of this kind of columns does not require any especial
improvement of the limit of detection when compared with the one chromatographic equipment.
obtained with analytical conventional columns.
In some cases it may be necessary to optimize the system with
High resolution the use of adequate conductions to minimize the efficiency losses
Columns of 15-25 cm length packaged with 3 µm packs achieve due to extra-column dead volumes. Besides, thanks to the elevated
efficiencies of over 30000 N/column, which can be very useful number of plates (N/col) of these columns, it can be tolerated a
when very complex samples require high reparation capabilities. certain loss of efficiency due to the system, without affecting greatly
to the resolution.
Sample:
1. Uracil
2. Naphtalene
3. Acenaphthene
4. Anthracene
140,000 N m
4.900 N column
Time of analysis: 40 seg.
Column: Tracer Extrasil

ODS2 3µ 3,5 x 0,46 cm
Eluyent: Acetonitrile/H20 65:35

Flow: 4.0 ml/min
Pressure: 110 bar
Sample volume: 10 µl
Temperature: Amb.
Detection: UV 254 nm
2 4 6 8
Minutes
Sample : Oligomeres of Polycarbonate

Column: Nucleosil 120 3µ C-18 25 x 0.4 cm
Eluyent: A: l0mM phosphoric acid
B: Acetonitrile
Gradient: 70-100% of B in 30 min
Flow: 3.0 ml/min
Temperature: 70 ºC; Detection: UV 200 nm
Time (min)
191
Preparative Columns
Preparative Columns
Preparative Chromatography
Teknokroma has developed the semi-preparative columns with the
same criteria of quality and versatility that has taken us to lead the
market of HPLC analytical columns.
Versatility
Teknokroma offers the highest range of phases of the market,
Charge capacity (mg)

Flow (ml/min)
covering practically all kind of functional groups. This simplifies

enormously the transposition from the analytical scale to the
preparative.
Besides, a complete range of dimensions of column, from 7.8 mm

to 21 mm of diameter, with lengths up to 25 cm and with a high
selection of particle sizes, makes it easy the definition of the ideal
configuration of column in relation to his volume capacity and the
kind of chromatographic equipment available in the laboratory.
Charge capacity (mg) Flow (ml/min)

Quality
Teknokroma has selected only those materials that offer the
maximum efficiency and reproducibility.
Each column is individually tested to guarantee that will fulfil the
high standards of quality demanded, controlling the parameters of
efficiency, peak symmetry and selectivity.
Analytical Quality Packing

The preparative columns packaged with 5 and 10 um analytical
packing offer exactly the same benefit levels than the correspondent
analytical columns.
Its high pressure packing ensures a high stability and consequently

a long life use of the column.
Preparative quality packings

The packing of preparative quality are the recommended for 20
mm ID or upper columns. These packings are manufactured under
the same quality standards, with the difference that they present a
particle size normally bigger and a size dispersion not as adjusted
as the analytical packings.
The result is an inferior cost of the column and, therefore, in many

cases an optimized cost for preparative separations.
Higher diameters of column available.
All kind of preparative packings and process packings.

Consult our technical department.
192
Guard Columns for HPLC
Guard Column 1 cm x 0,32 cm Guard Column 1 cm x 0,40 cm
(1 cm x 0,32cm) (1 cm x 0,40 cm)
Reference Description Reference Description

TR-C-160 Holder for 1 cm guard columns TR-C-160 Holder for 1 cm guard columns
TR-C-160K1 Holder + 2 cartridges ODS TR-C-160K1-4 Holder + 2 cartridges ODS
TR-C-160K2 Holder + 2 cartridges Si TR-C-160K2-4 Holder + 2 cartridges Si
TR-C-160K3 Holder + 2 cartridges C8 TR-C-160K3-4 Holder + 2 cartridges C8
TR-C-160K4 Holder + 2 cartridges NH2 TR-C-160K4-4 Holder + 2 cartridges NH2
TR-C-160K5 Holder + 2 cartridges SAX TR-C-160K5-4 Holder + 2 cartridges SAX
TR-C-160K6 Holder + 2 cartridges CN TR-C-160K6-4 Holder + 2 cartridges CN
TR-C-160K7 Holder + 2 cartridges PAH TR-C-160K7-4 Holder + 2 cartridges PAH
TR-C-160K8 Holder + 2 cartridges C6H5 TR-C-160K8-4 Holder + 2 cartridges C6H5
TR-C-160K9 Holder + 2 cartridges CARBOHYDRATES TR-C-160K9-4 Holder + 2 cartridges CARBOHYDRATES
TR-C-160K10 Holder + 2 cartridges ANION TR-C-160K10-4 Holder + 2 cartridges ANION
TR-C-160K11 Holder + 2 cartridges SCX TR-C-160K11-4 Holder + 2 cartridges SCX
TR-C-160K13 Holder + 2 cartridges 300 C4 TR-C-160K13-4 Holder + 2 cartridges 300 C4
TR-C-160K15 Holder + 2 cartridges DIOL TR-C-160K15-4 Holder + 2 cartridges DIOL
TR-C-160K18 Holder + 2 cartridges PRP-1 TR-C-160K18-4 Holder + 2 cartridges PRP-1
TR-C-160K19 Holder + 2 cartridges PEPTIDE C18 TR-C-160K19-4 Holder + 2 cartridges PEPTIDE C18
TR-C-160-1 ODS Cartridges (5 units) TR-C-160-1-4 ODS Cartridges (5 units)
TR-C-160-2 Si Cartridges (5 units) TR-C-160-2-4 Si Cartridges (5 units)
TR-C-160-3 C8 Cartridges (5 units) TR-C-160-3-4 C8 Cartridges (5 units)
TR-C-160-4 NH2 Cartridges (5 units) TR-C-160-4-4 NH2 Cartridges (5 units)
TR-C-160-5 SAX Cartridges (5 units) TR-C-160-5-4 SAX Cartridges (5 units)
TR-C-160-6 CN Cartridges (5 units) TR-C-160-6-4 CN Cartridges (5 units)
TR-C-160-7 PAH Cartridges (5 units) TR-C-160-7-4 PAH Cartridges (5 units)
TR-C-160-8 C6H5 Cartridges (5 units) TR-C-160-8-4 C6H5 Cartridges (5 units)
TR-C-160-9 CARBOHYDRATES Cartridges (5 units) TR-C-160-9-4 CARBOHYDRATES Cartridges (5 units)
TR-C-160-10 ANION Cartridges (5 units) TR-C-160-10-4 ANION Cartridges (5 units)
TR-C-160-11 SCX Cartridges (5 units) TR-C-160-11-4 SCX Cartridges (5 units)
TR-C-160-13 300C4 Cartridges (5 units) TR-C-160-13-4 300C4 Cartridges (5 units)
TR-C-160-15 DIOL Cartridges (5 units) TR-C-160-15-4 DIOL Cartridges (5 units)
TR-C-160-18 PRP-1 Cartridges (5 units) TR-C-160-18-4 PRP-1 Cartridges (5 units)
TR-C-160-19 PEPTIDE C18 Cartridges (5 units) TR-C-160-19-4 PEPTIDE C18 Cartridges (5 units)
193
Guard Column 2 cm x 0,32 cm Guard Column 2 cm x 0,40 cm
(2 cm x 0,32 cm) (2 cm x 0,40 cm)
Reference Description Reference Description

TR-C-460 Holder for 2 cm guard columns TR-C-460 Holder for 2 cm guard columns
TR-C-460K1 Holder + 2 cartridges ODS TR-C-460K1-4 Holder + 2 cartridges ODS
TR-C-460K2 Holder + 2 cartridges Si TR-C-460K2-4 Holder + 2 cartridges Si
TR-C-460K4 Holder + 2 cartridges NH2 TR-C-460K4-4 Holder + 2 cartridges NH2
TR-C-460K5 Holder + 2 cartridges SAX TR-C-460K5-4 Holder + 2 cartridges SAX
TR-C-460K6 Holder + 2 cartridges CN TR-C-460K6-4 Holder + 2 cartridges CN
TR-C-460K7 Holder + 2 cartridges PAH TR-C-460K7-4 Holder + 2 cartridges PAH
TR-C-460K8 Holder + 2 cartridges C6H5 TR-C-460K8-4 Holder + 2 cartridges C6H5
TR-C-460K9 Holder + 2 cartridges CARBOHYDRATES TR-C-460K9-4 Holder + 2 cartridges CARBOHYDRATES
TR-C-460K10 Holder + 2 cartridges ANION TR-C-460K10-4 Holder + 2 cartridges ANION
TR-C-460K11 Holder + 2 cartridges SCX TR-C-460K11-4 Holder + 2 cartridges SCX
TR-C-460K15 Holder + 2 cartridges DIOL TR-C-460K15-4 Holder + 2 cartridges DIOL
TR-C-460K18 Holder + 2 cartridges PRP-1 TR-C-460K18-4 Holder + 2 cartridges PRP-1
TR-C-460K19 Holder + 2 cartridges PEPTIDE C18 TR-C-460K19-4 Holder + 2 cartridges PEPTIDE C18
TR-C-460-1 ODS Cartridges (5 units) TR-C-460-1-4 ODS Cartridges (5 units)
TR-C-460-2 Si Cartridges (5 units) TR-C-460-2-4 Si Cartridges (5 units)
TR-C-460-4 NH2 Cartridges (5 units) TR-C-460-4-4 NH2 Cartridges (5 units)
TR-C-460-5 SAX Cartridges (5 units) TR-C-460-5-4 SAX Cartridges (5 units)
TR-C-460-6 CN Cartridges (5 units) TR-C-460-6-4 CN Cartridges (5 units)
TR-C-460-7 PAH Cartridges (5 units) TR-C-460-7-4 PAH Cartridges (5 units)
TR-C-460-8 C6H5 Cartridges (5 units) TR-C-460-8-4 C6H5 Cartridges (5 units)
TR-C-460-9 CARBOHYDRATES Cartridges (5 units) TR-C-460-9-4 CARBOHYDRATES Cartridges (5 units)
TR-C-460-10 ANION Cartridges (5 units) TR-C-460-10-4 ANION Cartridges (5 units)
TR-C-460-11 SCX Cartridges (5 units) TR-C-460-11-4 SCX Cartridges (5 units)
TR-C-460-15 DIOL Cartridges (5 units) TR-C-460-15-4 DIOL Cartridges (5 units)
TR-C-460-18 PRP-1 Cartridges (5 units) TR-C-460-18-4 PRP-1 Cartridges (5 units)
TR-C-460-19 PEPTIDE C18 Cartridges (5 units) TR-C-460-19-4 PEPTIDE C18 Cartridges (5 units)
194
Guard Columns for HPLC Columns Europa™ Guard Columns
• Interposed between the injector and the column these
Product Description Cat.Nbr.
precolumns lengthen the life of the column and improve the
Ultrafilter™, Ultrafit prefilter adaptor TR-010067
reproducibility of their results.
• Packed with the most modern HPLC packings and (frit not included)
Novabond™
proprietary packing procedures. Frits of 0.5 µm pore (10 units) TR-010069
• Economic and easily replaced. Frits of 2.0 µm pore (10 units) TR-010070
• For general use in any HPLC system.
• Packed at high pressure for maximum stability and duration. Ultraguard™, Ultrafit Guardcolumn adaptor TR-010068
• Their use does not imply any loss of efficiency, even with (guard column not included)
packings of 3 µm or with microbore columns of 2mm ID

Guard Column Peptide C18 10 x 3.2 mm (5 units) TR-C-160-19
BIOCOMPATIBLE Guard Columns Guard Column Protein 300 C18 10 x 3.2 mm (5 units) TR-C-160-16
• 100% biocompatible. Guard Column Protein 300 C8 10 x 3.2 mm (5 units) TR-C-160-14
• Economical cartridge system with titanium frits. Guard Column Protein 300 C4 10 x 3.2 mm (5 units) TR-C-160-13
Constructed in PEEK® and packed with de-activated silica: the

steel holder also ensures a total biocompatibility by having every
µm in contact with the mobile phase made of PEEK®. Tracer Excel™ Guard Columns
Guard Column Cartridges, Biocompatible Product Description Cat.Nbr.

2.0mm ID X 1 cm, 10 µm Ultrafilter™, Ultrafit prefilter adaptor TR-010067
UP-C-280 Reversed Phase C18 3-pk (frit not included)

UP-C-282 Reversed Phase C18 10-pk
UP-C-753 Absorption Si 3-pk Frits of 0.5 µm pore (10 units) TR-010069
UP-C-754 Absorption Si 10-pk Frits of 2.0 µm pore (10 units) TR-010070
UP-C-755 Amino Phase NH2 3-pk
UP-C-756 Amino Phase NH2 10-pk
UP-C-757 Cyano Phase CN 3-pk
Ultraguard™, Ultrafit Guardcolumn adaptor TR-010068
(guard column not included)
Guard Column Cartridges, Biocompatible
4.3mm ID X 1 cm, 5 µm Guard Column ODS 10 x 3.2 mm (5 units) TR-C-160-1
UP-C-750 Reversed Phase C18 3-pk Guard Column Si 10 x 3.2 mm (5 units) TR-C-160-2
UP-C-752 Reversed Phase C18 10-pk Guard Column C8 x 3.2 mm (5 units) TR-C-160-3
UP-C-759 Absorption Si 3-pk Guard Column NH2 10 x 3.2 mm (5 units) TR-C-160-4
UP-C-760 Absorption Si 10-pk Guard Column CN 10 x 3.2 mm (5 units) TR-C-160-6
UP-C-761 Amino Phase NH2 3-pk Guard Column Ph 10 x 3.2 mm (5 units) TR-C-160-8
UP-C-762 Amino Phase NH2 10-pk Guard Column C4 10 x 3.2 mm (5 units) TR-C-160-17
UP-C-763 Cyano Phase CN 3-pk Guard Column C1 10 x 3.2 mm (5 units) TR-C-160-20
Guard Column Cartridge Holders, Biocompatible mediterranea™ sea Guard Columns

UP-C-270 High Pressure, Stainless Steel, with (2) F-200 Fittings
UP-C-283 Low Pressure, Teflon, with (2) P-200/P-245 Fittings Product Description Cat.Nbr.
Ultrafilter™, Ultrafit prefilter adaptor TR-010067
Cartridge Guard Column Kits (frit not included)
UP-C-281 2.0mm ID C18 Cartridges (6-pk) with (1) C-270 Assembly
UP-C-751 4.3mm ID C18 Cartridges (6-pk) with (1) C-270 Assembly Frits of 0.5 µm pore (10 units) TR-010069
Frits of 2.0 µm pore (10 units) TR-010070
195
Ultraguard™, Ultrafit Guardcolumn adaptor TR-010068 Spares for Guard Column cartridges
Reference Description
TR-C-1030 Stainless steel frit 2 µm
Guard Column Sea18 10 x 3.2 mm (5 units) TR-010071 TR-C-1031 Titanium frit 2 µm
Guard Column Sea8 10 x 3.2 mm (5 units) TR-010073
Guard Column Sea4 10 x 3.2 mm (5 units) TR-010074 Preparative Guard Column
• Valuable prep column
COLUMN-GUARD COLUMN CONNECTOR
protection, 20-50mm ID
• Economical
• Low Pressure Drop
• Minimum dead volume
• High performance sample
• This column-Guard Column
distribution mechanism
connector is the ideal solution for
this type of connection, as its
dead volume is practically negligible. Reference Description
TR-C-260 Preparative Holder

Column-Guard Column connectors U-284 Union Column- TR-C-260K1 Prep cartridge ODS (2 units) + TR-C-260 Preparative Holder
Precolumn TR-C-260K2 Prep cartridge Peptide C18 (2 units) + TR-C-260 Preparative
Reference Description Holder
TR-C-260K3 Prep cartridge C8 (2 units) + TR-C-260 Preparative Holder
UP-U-284 Fingertight F-200 coupler, Delrin®, and .007¨ ID
TR-C-260K4 Prep cartridge NH2 (2 units) + TR-C-260 Preparative Holder
stainless steel tubing.
TR-C-260K6 Prep cartridge CN (2 units) + TR-C-260 Preparative Holder
UP-U-287 Fingertight F-300 coupler, PEEK®, and .007¨ ID
TR-C-260K13 Prep cartridge Protein C4 (2 units) + TR-C-260 Preparative Holder
stainless steel tubing. TR-C-260K14 Prep cartridge Protein C8 (2 units) + TR-C-260 Preparative Holder
TR-C-260K16 Prep cartridge Protein C18 (2 units) + TR-C-260 Preparative
Semipreparative Guard Columns
Holder
TR-C-260K17 Prep cartridge Si (2 units) + TR-C-260 Preparative Holder
• For semipreparative HPLC and SFC 1 cm I.D.
TR-C-260K18 Prep cartridge Mediterranea Sea 18 (2 units) + TR-C-260
Preparative Holder
TR-C-260-1 Prep cartridge ODS (2 units)

TR-C-260-2 Prep cartridge Peptide C18 (2 units)
TR-C-260-3 Prep cartridge C8 (2 units)
TR-C-260-4 Prep cartridge NH2 (2 units)
TR-C-260-6 Prep cartridge CN (2 units)
UP-C.1000. Holder for TR-C-260-13 Prep cartridge Protein C4 (2 units)
semipreparative cartridge TR-C-260-14 Prep cartridge Protein C8 (2 units)
Reference Description TR-C-260-16 Prep cartridge Protein C18 (2 units)
UP-C-1000 Semi-Prep holder TR-C-260-17 Prep cartridge Si (2 units)
TR-C-360K1 Semi-Prep cartridge ODS (2 units) + UP-C-1000 holder TR-C-260-18 Prep cartridge Mediterranea Sea 18 (2 units)
TR-C-360K2 Semi-Prep cartridge Si (2 units) + UP-C-1000 holder
TR-C-360K3 Semi-Prep cartridge C8 (2 units) + UP-C-1000 holder
TR-C-360K4 Semi-Prep cartridge NH2 (2 units) + UP-C-1000 holder Iso-Prep™ Filter for Preparative Columns
TR-C-360K6 Semi-Prep cartridge CN (2 units) + UP-C-1000 holder
TR-C-360K13 Semi-Prep cartridge Protein C4 (2 units) + UP-C-1000 holder
TR-C-360K17 Semi-Prep cartridge Peptide C18 (2 units) + UP-C-1000 holder
TR-C-360K18 Semi-Prep cartridge Mediterranea Sea 18 (2 units)
+ UP-C-1000 holder • Economical protection for preparative HPLC column
TR-C-360-1 Semi-Prep cartridge ODS (2 units) and injector
TR-C-360-2 Semi-Prep cartridge Si (2 units) • Precolumn/Inline filter functionality
TR-C-360-3 Semi-Prep cartridge C8 (2 units) • Stable to 8,000 psi
TR-C-360-4 Semi-Prep cartridge NH2 (2 units) • Replaceable filters
TR-C-360-6 Semi-Prep cartridge CN (2 units)
TR-C-360-13 Semi-Prep cartridge Protein C4 (2 units)
TR-C-360-14 Semi-Prep cartridge Protein C8 (2 units) TR-C-260-F In Line Filter
TR-C-360-16 Semi-Prep cartridge Protein C18 (2 units) TR-C-260-FX Replacement Filter (10 units)
TR-C-360-17 Semi-Prep cartridge Peptide C18 (2 units)
TR-C-360-18 Semi-Prep cartridge Mediterranea Sea 18 (2 units)
196
mediterranea™ Sea HPLC Column
Introduction
Today there is still a consensus about the fact that the best material
The mediterranea™ sea18 column provides a performance level to use as chromatographic packing continues to be silica. The
that, until now, has not been reached in efficiency, inertness, pH- particles of this material are very physically resistant, enable
robustness, reproducibility and reliability. mediterranea™ sea18 multiple functions, present maximum levels of efficiency and are
columns simplify and make your HPLC work more pleasant. You also compatible with practically all solvents.
won’t worry about the extreme basic or acidic natures of your
samples with the mediterranea™ sea18 column. Teknokroma has been concentrated on obtaining the best silica
particle in the market. The silica particle on which the mediterranea™
The versatility of the mediterranea™ sea18 column will enable you sea18 column is based is the result of an optimisation process in
to deal successfully with the immense variety of separations in the which, starting off from extremely pure materials with unusual low
fields of pharmaceuticals, life sciences, environment, foods, etc. metal content, a perfectly spherical, rigid and inert particle has been
obtained. Furthermore, the “porification” process developed for
Once every ten years, the world of chromatography experiences a these ends (Surface Enhanced Accessibility, SEA) has achieved
revolutionary technology that surpasses all others and meets the a high surface without losing any of its properties of physical
expectations of chromatographic scientists. resistance while also showing a very high load capacity, ideal
for preparatory scaled processes. Moreover, the obtained porous
Teknokroma has focused all its efforts and all its know-how, structure ensures the maximum transfer speed of the solutes
accumulated through more than 30 years of chromatographic between the stationary and mobile phases, resulting in a greater
research and development, in offering the global-best reverse separation efficiency.
phase HPLC packing mediterranea™ sea18.
Let us demonstrate the superior chromatographic properties of the
While developing the mediterranea™ sea18 column we created mediterranea™ sea18 column, so you will feel comfortable with the
two novel proprietary bonding & packing technologies. In order to performance of the world’s best reverse-phase HPLC column.
demonstrate the global-best technology of mediterranea™ sea18,
we compared chromatographic results from the world’s most
popular reverse-phase HPLC columns. We invite you to try our
mediterranea™ sea18 when you experience less-than-satisfactory
results with your favourite column.
197
Purity of Silica More than 98% of the silica surface area responsible for
After evaluating many materials as a base for the global-best chromatographic separation of the sample is found inside the
reverse phase chromatographic packing, the clear consensus is particle - within the pores. This explains the extreme importance
that the special characteristics of silica packings classify them of obtaining a very homogeneous pore distribution and the least
as unsurpassable. No other packing material, apart from ultra- possible number of nanopores. For most reverse-phase silica
pure silica, achieves the perfect balance of physical resistance, packings, these nanopores are not properly chemically bonded,
functional use, chemical inertness, reproducibility and efficiency. endcapped or deactivated. So when nanopores are accessible
Ultra-pure silica is also compatible with practically all solvents. to analytes, surface-analyte interactions frequently dominate.
Teknokroma concentrated on presenting the best silica particle to These surface-analyte interactions slow down the chromatographic
the HPLC market. process (“load transfer”), often resulting in decreased column
efficiency. These treacherous nanopores may also negatively
An essential condition for obtaining the global-best reverse phase influence the phenomenon of dewetting which occurs with totally
packing is an extremely pure silica. The silica particle, on which aqueous mobile phases.
the new mediterranea™ sea18 packing is based, is obtained from
ultra-pure materials, using rigorously controlled manufacturing Multifunctional Endcapping Deactivation (MED)
processes to ensure that the slightest possibility of contamination The endcapping process is a critical step in obtaining a perfectly
is avoided. The mediterranea™ sea18 silica required intensive deactivated mediterranea™ sea18 column. Our proprietary
optimisation of numerous processing factors to achieve a perfectly Multifunctional Endcapping Deactivation (MED) technology
spherical, rigid and inert particle possessing unusually low metal maximizes surface-bonding, blocking practically all the active
content. The almost total absence of metals is one of the pillars centres that may have remained on the surface of the silica after
over which the extraordinary properties of the mediterranea™ bonding the C18 chains. Thanks to our new MED technology,
sea18 column reside. the mediterranea™ sea18 column enjoys an unusual low level of
silanol activity - helping you to obtain symmetrical peaks from even
Metals Content (ppm) the most basic and acidic pharmaceuticals and their metabolites.
Metal Values Obtained mediterranea™ sea18 bonding chemistries will help you to achieve
Al <1ppm
an extraordinary resistance and column lifetime when running at
extreme pH levels.
Fe <1ppm
Ti <1ppm
Moreover, the mediterranea™ sea18 column has been designed
Zr <1ppm to show an excellent retention of polar compounds in a 100%
aqueous environment without the problems of unwanted
Porosity (Surface Enhanced Accessibility, SEA) interactions which inefficiently endcapped conventional packings
produce. Packing chemistry based on the new MED technology,
3000% “multifunctional endcapping deactivated”, achieves levels of
Pore Size deactivation, resistance to extreme pH values and versatility in its
2500% chromatographic applications never reached by conventional or
polar-embedded reverse phase packings. The MED technology has
2000% been rigorously developed to achieve the maximum reproducibility,
with the objective that its chromatographic separations will be,
1500%
column to column, exactly the same.
1000% The obtained deactivation is shown when we make chromatograms

of a group of Basic compounds in neutral pH conditions, including
500% a neutral compound (acenaphthene) as a comparison. Of the four
tested columns, the mediterranea™ sea18 is the one that shows
0% the greatest efficiency, whether measuring with the acenaphthene
0 20 40 60 80 87 100 120 140 160 180 200 or with a peak as difficult as that of amitriptilyne. The same occurs
The pore distribution of the mediterranea™ sea18 column has if we compare the asymmetry values of the peaks.
been optimised by our own proprietary process called Surface Column As Ncol As Ncol
Enhanced Accessibility (Sea). The Surface Enhanced Accessibility Acenaphtene Acenaphtene Amitryptiline Amitriptilyne
“porification” process creates high surface area without losing silica mediterranea™ sea18 5 µm 15 x 0,46 1,06 11031 1,21 8119
structural strength, chemical resistance, chemical inertness and Xterra MSC18 5 µm 15 x 0,39 1,36 6476 1,32 4619
high load capacity. Surface Enhanced Accessibility also ensures
Gemini C18 5 µm 15 x 0,46 1,22 9524 1,23 7490
that practically 100% of the internal packing surface has been
Nucleosil 100 C18 5 µm 15 x 0,46 1,07 7815 na na
chemically bonded, endcapped, and is accessible to compounds
being separated. Moreover, the Surface Enhanced Accessibility of
mediterranea™ sea18 ensures the maximum transfer speed of the
solutes between the stationary and mobile phases, resulting in a
greater separation efficiency.
198
Tricyclic Anti-depressants Aqueous Environments
mAU
The mediterranea sea18 packing is a 100% pure reverse phase
Peaks:
3 with the added advantage of showing excellent retention of polar
1. Desipramine
2. Nortriptyline compounds and also enables work with 100% aqueous mobile
3. Acenaphthene phases without any limitation.
4. Imipramine
5. Amitrptyline
Most chromatographers agree that polar embedded packing have
2 6. Trimipramine
1 5
4 an advantage over conventional packings, in that they can work
6 in 100% aqueous environments and separate basic compounds.
Nevertheless, these advantages are achieved at the expense of

less retention for polar compounds, and poor column stability.
Polar-embedded packings exhibit chromatographic behavior that
min
cannot be considered as 100% reverse phase, since secondary
Column: mediterranea sea 18, 5 µm 15 x 0,46 cm interaction mechanisms may co-exist due to the nature of the
Eluent: Methanol/20mM K2HPO4 (pH 7.0) 70:30 unspecified polar groups anchored at the base of the hydrocarbon
Flow: 1ml/min
chains.
Room Temperature
Detection: UV 254 nm
The mediterranea sea18 packing surpasses all the advantages of
Basic Compounds polar embedded packings by a wide margin and shows none of its
inconveniences.
mAU Furthermore, due to its specially optimised endcapping process
3 Composition of test solution: (MED), the column has guaranteed pH-resistance, reproductibility
1. Propanolol and long life.
1 2. Diphenhydramine
3. Acenaphthene
4. Amitrityline As can be seen, the chromatograms that are obtained after eluting
4 the column with 100% water for more than 40 hours show no
appreciable alteration in the retention times or in the efficiency of
2
the chromatographed peaks.
The mediterranea™ sea18 column also widely surpasses the

stop flow test, designed to be able to show up the dewetting
phenomenon that usually occurs in highly deactivated ODS-type
min columns, causing irreversible expulsion of water included in the
packing pores. As can be seen in the data of five successive Stop
Column A - mediterranea™ sea 18 Flow Test cycles no significant alterations are observed in the
mAU
chromatographed peaks.
3 Composition of test solution:
1. Propanolol
Aqueous Environments
2. Diphenhydramine
3. Acenaphthene 50000 2 50000
4. Amitrityline 1 Peaks: 2 Peaks:
1. Citosine 1 1. Citosine
40000 2. Uracil 40000 2. Uracil
3. Cytidine 3. Cytidine
4. Uridine 4. Uridine
2 5. Thymine 5. Thymine
4 30000 30000
3 5 5
4 3
20000 20000 4
min
10000 10000
Column B - The Competition 0 5 10 15 20

0 5 10 15 20
Initial Final after 40 hours of 100% H2O
Column A: mediterranea sea 18, 5 µm 15 X 0,46 cm
Column B: Other column from market 5 µm 15 x 0,46 cm
Eluyent: Methanol/0,02M K2HPO4/KH2PO4 pH7,00 (75:25) Column: mediterranea sea18 5 µm 15 X 0,46 cm
Room temperature Movil Phase: H2O
Flow: 1.4 ml/min Flow: 1ml/min
Detection: UV 254 nm Vol. Iny.:10 µl
Detectión: UV 254 nm
199
Mobile Phase
MeOH/H2O H2O Methanol
Pore
Silica Particle A B C
The phenomenon of “Dewetting” Compound 1st stop 2nd stop 3rd stop 4th stop 5th stop
When working with mixed mobile phases of an organic phase and tR initial flow flow flow flow flow
water, for example Methanol/H2O, the pores of the packings are Cytosine 3,32 3,33 3,3 3,35 3,16 3,21
totally occupied with the mobile phase (A). However, when working Uracil 4,45 4,45 4,44 4,75 4,36 4,44
with 100% H2O as the mobile phase in conventional reverse-phase Cytidine 7,73 7,73 7,63 8,00 7,24 7,34
columns, a phenomenon occurs with the expulsion of the mobile
Uridine 11,57 11,57 11,53 12,02 11,25 11,24
phase from the interior of the pore (B).The chromatographic effect
Thymine 12,70 12,7 12,62 12,87 12,35 12,70
that will be produced is a loss of retention and resolution of the
chromatographic peaks since the solutes cannot enter the interior
of the pores. These chromatographic losses can occur gradually
or suddenly - making it difficult to restore to its initial conditions, NIST Test for HPLC Packing Characterization
especially with mostly aqueous mobile phases. (C). The new mediterranea™ sea18 column has been subjected to the
SRM870 test. This test, designed by the NATIONAL INSTITUTE
This phenomenon is ruled by an equation which involves the OF STANDARDS & TECHNOLOGY and recently assessed by the
pore’s radius, the surface tension, the contact angle and the experts committee of the USP (United States Pharmacopeia) is
pressure exercised on the mobile phase. The surface tension and currently considered to be the most recommended for evaluating
contact angle depends on the density of the bonded ligands and the most significant properties of a reverse phase column.
on their chemical functionality. The Stop Flow Test reproduces
chromatographic run conditions by interrupting the flow of 100% The high number of HPLC reverse phase packings available in the
aqueous mobile phase, the pressure goes to zero and favours the market and the big differences in their chromatographic behaviour
expulsion of water from the interior of the pores. has led to the need to design a characterisation and classification
method for these packings.
The mediterranea™ sea18 column surpasses this test with ease - This procedure uses a mixture of five organic components (uracil,
the retention times of the five chromatographed compounds remain toluene, ethylbenzene, quinizarin and amitriptyline) which are
practically unaltered. chromatographed using exact conditions of mobile phase, flow, and
controlled temperatures.
Stop Flow
The detailed analysis of the different peaks obtained will enable
an objective, and more importantly, standardised evaluation of the
14 behaviour of the chromatographic packing and therefore anticipate
12
its suitability in normal analytical work.
10
8
6
4
2 5 Peaks
1. Uracil
0
tR Initial 1st. Stop Flow 2º Stop Flow 3er Stop Flow 4º Stop Flow 5º Stop Flow
1 2. Toluene
3. Ethylbenzene
2
4. Quinizarin
3 5. Amitriptyline
4
Cytosine Uracil Cytidine Uridine Thymine
200
normally elutes between the ethylbenzene and amitriptyline peaks.
uracil - dead volume marker However, when the silica packing contains embedded polar groups
they will retain this peak, causing it to elute after amitriptyline. In
the mediterranea™ sea18 column, the quinizarin peak elutes with
a perfect symmetrical form, indicating an extraordinary low level of
toluene - hydrophobic retention metallic impurities.
According to quinizarin peak symmetry data obtained in our
laboratories or published by the NIST (see Figure), the performance
of the mediterranea™ sea18 column compares well with other
ethylbenzene - methylene selectivity popular reverse-phase packings.
The top-positioning of the mediterranea™ sea18 packing indicates
the ultra-high purity of the optimized silica.Teknokroma´s ultra-pure
quinizarin - activity towards chelating reagents silica is your guarantee of reproducibility and of the absence of
secondary (and uncontrolled) mechanisms of interaction (common
to popular polar-embedded columns).
amitriptyline - activity towards bases Amitriptyline

This basic (pKa=9.4) anti-depressant is an excellent indicator of
residual silica surface silanol-activity. Amitriptyline will elute as a
symmetrical peak on a well-deactivated column as seen with the
new mediterranea™ sea18. In comparison, many popular reverse-
Uracil phase packings leave many residual silanols through insufficient
This compound is commonly used as an indicator of the dead endcapping; leading to widespread peak tailing or to complete
volume of the column (non-retained peak). disappearance from the chromatogram.
Proper amitriptyline elution is important in consideration of
Toluene/Ethylbenzene the number of basic compounds, particularly in the fields of
The selectivity factor between these two compounds can be used pharmaceuticals and life science. In fact, it guarantees that the
to characterise the differences between packings primarily due to problems with tailing or complete peak disappearance will be
solvophobic interactions. The absolute retention times of these almost eradicated - along with day-to-day laboratory adjustments
compounds give an idea of the column reverse-phase strength. and complex mobile phase systems designs. With mediterranea™
Both compounds can also be used to measure the quality of the sea18 a simple pH adjustment will serve to correctly elute the most
packing through the number of theoretical plates. basic and acidic substances.
The comparison of asymmetry factors for mediterranea™ sea18
Quinizarin (1,4-dihydroanthraquinone) and other popular packings is a clear indication of deactivation.
Quinizarin is a chelating compound and its behaviour in a reverse mediterranea™ sea18 enters the market with a deactivation level
phase column is a clear indicator of the presence or absence of not previously achieved by other reverse-phase packings. The
metals. A column of low activity will deliver symmetrical peaks proprietary Multifunctional Endcapped Deactivation produces
whereas increasing surface activity exaggerates the tailing edge of reproducible column-to-column peak symmetry for a wider variety
the quinizarin peak - leading to higher asymmetry values. Quinizarin of pharmaceutical compounds thanks to strict silica purity and
batch-to-batch reproducibility.
201
Packaging Sample
Wide pH Range
A perfectly spherical particle, a totally controlled pore design, a total
lack of metallic traces, a well-studied process of phase bonding
and final endcapping, all combine in achieving a packing with a
resistance to extreme pH values not previously reached.
Until quite recently, silica packings were limited to working between

pH 2 and pH 7 since below pH 2 the bonds between the C18 chains
and the silica particle were hydrolysed, resulting in a gradual loss
of retention capacity of the column. Above pH 7 the problem that
arose was one of simply dissolving the silica, and therefore the
pure destruction of the column.
Using mediterranea™ sea18 packing makes it is possible to work

with eluents from pH 1 to pH 12. Such unusual pH-resistance
values have been secured as a result of phase bonding efficiency
and a proprietary endcapping process which provides a protective
shield that impedes acidic and basic eluents from attacking the
silica surface.
The pH stability graphs show the efficiency of the process.
Eluting the mediterranea™ sea18 column for 78 hours at pH 11.5,

showed no significant deterioration in terms of both efficiency and
peak symmetry for diphenhydramine..
An eluent of ACN//1- methylpyrrolidine 50mM pH 11,5 50:50, 1ml/min 25ºC is
With an eluent as acid as pH 1, the column stabilises in a short passed through the column. With the same eluent 10 ml of diphenhydramine (1mg/
period of time so that it will even be possible to work in these ml dissolved in water) is injected and the efficiency and symmetry of the peak is
tested.
extreme conditions.
LC-MS Mediterranea™ Sea 18 Columns

The Multifunctional Endcapping Deactivation (MED) technology
guarantees extreme stability for every mediterranea™ sea18
reverse-phase column.
Chromatographic stability (peak symmetry, peak retention times,
and peak efficiency) under low-to-high pH (pH 1-12) conditions
is required for high-speed, high-throughput LC-MS. The
mediterranea™ sea18 is the ideal LC-MS reverse-phase column
for stable chromatographic separation of pharmaceuticals and
their metabolites.
The technological features designed into the mediterranea™
sea18 column makes it extremely useful for LC-MS applications
where packing stability is demonstrated by low column bleed
and consistent chromatographic results. The combination of
mediterranea™ sea18 technology on a 3mm ultra-pure silica-
An eluent of ACN/TFA1% pH 1.0 (10:90) 1ml/min 25ºC is passed through the column based packing enables LC-MS separations to be made speedily
at regular periods, checked with the reverse phase test and a retention comparison is and with maximum productivity.
made of the last anthracene peak.
202
Bleeding Profile Comparison
100
Blank
0
100
mediterranea sea
0
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00
Assay by Instituto Químico de Sarriá I.Q.S. (Barcelona)
Chromatographic Conditions Conditions for MS Detection

Mobile Phase: A: CH3CN (0,1% formic acid) MS Instrument: Waters ZMD
B: Water (0,1% formic acid) Capillary Voltage: 3kV (ESI positive)
Elution Gradient: 5/95(A/B) linear up to 95:5 in 8 minutes, Cone Voltage: 20V
maintaining the final composition 2 minutes. Source block Temp: 100ºC
Flow: 0,5mL/min Desolvation Temp: 350ºC
Column Temperature: 25ºC Gas: 500l/h
Gas of cone: 35 l/h
Mass Range: 60 to100 amu
Ultra-Rapid Columns
Within the wide range of possible configurations , the mediterranea™
sea18 columns are available with 3 mm packing with lengths of 3,
5 and 10 cm and inner diameters of 2.1, 3.0, 4.0 and 4.6 mm. By
maintaining high quality control and specifications in manufacturing
the mediterranea™ sea18 packing, these columns enable you to
do ultra-fast separations, with extremely high levels of productivity
Composition of Test Solution
and reduced analysis times. Ultrarapid mediterranea™ sea18
Uracil (TM) 0.002 mg/ml
columns will help you optimize your instrument time and give you Naphthalene 0.100 mg/ml
more time to analyze data. Acenaphthene 0.200 mg/ml
Anthracene 0.004 mg/ml
With Ultra-rapid column separations, total analysis times of less

than one minute are common, even when using gradient elution
methods, since the high porosity of the mediterranea™ sea18
packing enables rapid mobile phase equilibration times.
The combination of 3 mm mediterranea™ sea18 packing with the

column diameter of 2.1 mm is recommended for high sensitivity LC/
MS analyses. Many of these ultra-rapid LC-MS screening analyses Chromatographic Conditions
utilize minute sample and solvent quantities - for which, the 3 mm Column: mediterranea sea18 3 µm 3 x 0,46 cm
mediterranea™ sea18 columns are ideal. Eluant: Acetonitrile/Water
Proportion: 65/35
Flow: 3.0 ml/min
Pressure: 70 bars
Vol Inyection: 0.5 ml
Temperature: Room
Detectión: UV 254 nm
203
Peaks:
2 1) Uracil (TM)
2) Naphthalene
3) Acenaphthene
4) Anthracene
3
4
Preparative Columns
The mediterranea™ sea18 columns are 1
characterized by their total inertness, by their wide

range of working mobile phase pH, and by their high loading 0 2 4 6
capacity - a result of the SEA process control (Surface Enhanced Minutes
Accessibility). Column with Ultrafit™ System
The mediterranea™ sea18 preparative columns are the natural 2
choice when high-service preparative columns are required, and in
high-speed preparative applications as in the case of Combinatorial
Chemistry.
3
New Hardware Design for Mediterranea™ Column: 4
Ultrafit™ System
The new Ultrafit™ design will make your work in the laboratory 1
more comfortable and efficient. The Ultrafit™ system, as well
as helping in the replacement of the frit at the column entrance,
0 2 4 6
enables you to easily include either additional frits or a pre-column, Minutes
always with the utmost simplicity and economy and in no way
Column with Ultrafit™ System + Ultrafilter™
whatsoever is the quality of the separation affected.
In designing the Ultrafit™ column, the greatest care has been taken 2
to cover all the aspects that may occur in the loss of efficiency of
the column. As a result of all this, dead volumes have been reduced
to a minimum, entered by the system by means of a high precision
mechanism, with inlet and outlet holes of 0.2 mm and first-class 3
tapers for the perfect distribution of the inlet and outlet flows, as 4
seen in the three depicted Ultrafit™ options. The Ultrafit™ system
enables a pre-column to be included without loss of efficiency, to 1
columns as small as 30 x 4 mm packed with particles of 3 mm.
Moreover, the very best material has been selected for the
construction of the column, with an ultra-shiny interior finish, of 0 2
Minutes
4 6
extremely low RMS, ensuring that no tube imperfection in the

Column with Ultrafit™ System + Ultraguard™
column will affect the quality of the separation.
Ultrafit™ System Efficiency

Ultrafit™ System Configuration
Efficiency AS
Column (N/m) (10%)
mediterranea sea18 Column 3 µm 134904 1,11 Ultrafit™

5 x 0,46 cm Ultrafit™ System
mediterranea sea18 Column 3 µm 135042 1,05
5 x 0,46 cm with Prefilter Ultrafilter™
mediterranea sea18 Column 3 µm 137819 1,07 Ultrafit™ + Ultrafiter™
5 x 0,46 cm with Precolumn Ultraguard™
Chromatographic Conditions:
Column: mediterranea™ sea18 3 mm 5 x 0,46 cm
Eluant: Acetonitryle/Water 65:35 UltrafitT™ + Ultraguard™
Flow: 0,9 ml/min
Det. UV 254 nm
Temp. Room
Sample: Acenaphthene 0.2 mg/ml
204
mediterranea™ Sea 5 µm HPLC Column
Analytical Columns 0.46 cm ID Microbore Columns 0.21 cm ID
mediterranea™ sea 5 µm mediterranea™ sea 5 µm
Length Diameter Length Diameter
Packing Funct. µm cm cm Cat.Nbr. Packing Funct. µm cm cm Cat.Nbr.
mediterranea Sea18 5 3 0.46 TR-010000 mediterranea Sea18 5 3 0.21 TR-010014
mediterranea Sea4 5 4 0.46 TR-010363
Microbore Columns 0.30 cm ID
mediterranea™ sea 5 µm
Length Diameter
Packing Funct. µm cm cm Cat.Nbr.
Analytical Columns 0.40 mm ID mediterranea Sea18 5 10 0.30 TR-010021
mediterranea™ sea 5 µm mediterranea Sea18 5 15 0.30 TR-010022
Length Diameter mediterranea Sea18 5 20 0.30 TR-010023
Packing Funct. µm cm cm Cat.Nbr. mediterranea Sea18 5 25 0.30 TR-010024

205
mediterranea™ Sea 3 & 5 µm HPLC Column
SemiPreparative Columns Ultrarapid Columns 0.46 cm ID
mediterranea™ sea 5 µm mediterranea™ sea 3 µm
mediterranea Sea8 5 15 212 TR-010411 mediterranea Sea4 3 15 0.46 TR-010442
mediterranea Sea8 5 25 2,12 TR-010412 mediterranea Sea4 3 20 0.46 TR-010443
mediterranea Sea4 5 25 0.78 TR-010415 Ultrarapid Columns 0.40 cm ID
mediterranea Sea4 5 10 1.00 TR-010416 mediterranea™ sea 3 µm
mediterranea Sea4 5 15 1.00 TR-010417 Length Diameter
mediterranea Sea4 5 25 1.00 TR-010418 Packing Funct. µm cm cm Cat.Nbr.

206
mediterranea™ Sea 3 µm HPLC Column
Microbore Columns 0.21 cm ID Other Products
mediterranea™ sea 3 µm mediterranea™ sea
Length Diameter Product Description Cat.Nbr.
Packing Funct. µm cm cm Cat.Nbr. Ultrafilter™, Ultrafit prefilter adaptor TR-010067
mediterranea Sea18 3 3 0.21 TR-010053 (frit not included)
mediterranea Sea18 3 10 0.21 TR-010055 Frits of 0.5 µm pore (10 units) TR-010069
mediterranea Sea18 3 15 0.21 TR-010056 Frits of 2.0 µm pore (10 units) TR-010070
Ultraguard™, Ultrafit Guardcolumn adaptor TR-010068

Microbore Columns 0.30 cm ID

mediterranea™ sea 3 µm
Length D
iameter
207
mediterranea™ Sea UHPLC Columns
mediterranea™ Sea18 1.8 μm mediterranea™ Sea18 2.2 μm
• Ideal for LC-MS applications • Reduces analysis costs with no resolution loss
• Fully scalable to Industrial Chromatography • Less backpressure than 1.8 μm columns
• Compatible with 100% aqueous mobile phases • High flow rates with no efficiency loss
• Robustness and versatility • Reduces solvent consumption
• Very high efficiency • Ideal for LC-MS applications
mediterranea™ sea18 1.8 μm columns have been developed mediterranea™ sea18 2.2 μm is a transitional column fully
based on the same material as the recognized 5μm column.The result is a compatible with cutting-edge HPLC and UHPLC systems, radically
column with high efficiency, high reliability and full scalability from UHPLC improving analysis performance and halving retention times.
analytical chromatography to industrial process chromatography. The material used for the developement of mediterranea™ sea18
mediterranea™ sea18 1.8 μm columns are available in 1.8, 2.2,3, 2.2 μm columns is an ultra-pure, metal-free, state-of-the-art silica gel
5, 10, and 15 μm. Its versatility allows the separation of both nonpolar and packing, with a particle size of 2.2 μm and a pore size of 100Å.
polar substances, and its robustness to extreme pH values (1.5 to 11) mediterranea™ sea18 2.2 μm uses state-of-the-art silica and
makes it the ideal column for methods development the latest bonding and endcapping technology. Compatible with a 100%
mediterranea™ sea18 1.8 μm has been developed to offer the aqueous mobile phase and it stands extreme pH conditions (1 to 12).
highest quality and reproducibility.Teknokroma checks rigorously both each mediterranea™ sea18 2.2 μm has been developed to offer the
packing batch and every single column. highest quality and reproducibility.Teknokroma checks rigorously both each
packing batch and every single column.
UHPLC Columns UHPLC Columns
mediterranea™ sea 1.8 µm mediterranea™ sea 2.2 µm
mediterranea Sea18 1.8 5 0.21 TR-010902 mediterranea Sea18 2.2 5 0.21 TR-010900
mediterranea Sea18 1.8 15 0.46 TR-010908
UHPLC Guardcolumns
UHPLC Guardcolumns Length Diameter
Length Diameter Packing Funct. µm cm cm Cat.Nbr.
Packing Funct. µm cm Holder + 2 Cartridges C18 2 1 0.21 GL-5020-20382
cm Cat.Nbr. 2 Cartridges C18 2 1 0.21 GL-5020-20331
Holder + 2 Cartridges C18 1.9 1 0.21 GL-5020-88246 Holder + 2 Cartridges C18 2 1 0.30 GL-5020-20399
2 Cartridges C18 1.9 1 0.21 GL-5020-88240 2 Cartridges C18 2 1 0.30 GL-5020-20348
Holder + 2 Cartridges C18 1.9 1 0.30 GL-5020-88248
2 Cartridges C18 1.9 1 0.30 GL-5020-88242 UHPLC Guardcolumn Filter
Pore Inter.Volume
Description µm µL Cat.Nbr.
Stainless Steel Precolumn Filter 0.5 0.61 JR-69330-05
Stainless Steel Precolumn Filter 2 0.66 JR-69330-2
GL-5020-20382 JR-69330-05
Stainless Steel Precolumn Filter 5 0.68 JR-69330-5
208
Brisa LC2 HPLC Column
Latest technology Ultrapure Silica

Optimizes your analysis costs
Immediate delivery Analytical
Column Brisa LC2
“Limited Cost x Liquid Chromatography”
The material used for this column development is an ultrapure and metal free silica packing.
The pore size is 120 A and it´s available in 3 & 5 um particle size.
Brisa “LC2” is a fully “endcapped” free silanol silica with a broad usable pH range (2-11).
Brisa “LC2” has been designed to get the highest reproducibility and quality.
Teknokroma strictly controls each packing batch and each single column.
Analytical Columns LC-MS Columns

Brisa LC2 3 & 5 µm Brisa LC2 C18-MS 3 & 5 µm
Length Diameter Length D
iameter
Brisa LC2 C18 5 15 0.46 TR-010480 Brisa LC2 C18-MS 3 5 0.21 TR-010496
Brisa LC 2
C18 3 10 0.46 TR-010499 Brisa LC 2
C18-MS 5 15 0.21 TR-010486
Brisa LC2 C18-MS 5 25 0.21 TR-010487
UHPLC Columns
Brisa LC2 C18-MS 5 µm
Length Diameter
Brisa LC2 C18-MS 1.8 5 0.21 TR-010490
Brisa LC2 C18-MS 2.2 5 0.21 TR-010493
209
Europa HPLC Column for Peptides and Proteins
Introduction
Teknokroma introduces in the market the new line of Europa Furthermore, the propietary “porification process” (Surface
HPLC columns. Enhanced Accessibility, SEA) for Europa silica has achieved
high surface area without sacrificing important properties like
After the versatility of our popular mediterranea™ column physical resistence and high loading capacity- making it ideal for
that enables you to deal successfully with the inmense variety preparative-scale processing.
of separations in the fields of pharmaceuticals, life sciences, In addition, the Surface Enhanced Accessibility manufacturing
environment, foods, etc. Teknokroma has focused all its efforts process creates a porous structure that ensures maximum transfer
and all its know-how, accumulated through more than 40 years of speeds for solutes between the stationary and mobile phases-
chromatographic research and development, in offering the best resulting in higher separation efficiency.
reverse phase HPLC packing for identification and purification of
peptides and protein compounds. Our “Ultra-Fast” Europa columns are made in 3-5 cm length in
order to get quick analytical results, whereas the “High Efficiency”
Manufactured using novel proprietary technologies, analytical and columns are normally in 15-25 cm lengths to obtain best resolution.
preparative Europa columns are simply the best reverse phase
columns available today. The Teknokroma Europa Columns are uniquely designed with
optimized pore size distribution; 120Å for Peptide and 300Å for
As a result of these, we launch into the market the Line of Europa the Protein Columns.
HPLC columns, one of the best columns in the field of analysis of
biomolecules. Europa columns are available for:
The Europa HPLC columns for peptides and proteins, provide
the best performance and unsurpassed efficiency, reliability and Peptides: Europa C18 with 0.21, 0.30, 0.40, 0.46, 0.78, 1.0 and
reproducibility. 2.12 cm.
There is still a consensus that the best material to use as Proteins: Europa C18, C8 and C4 with 0.21, 0.30, 0.40, 0.46,
chromatographic packing continues to be silica. The particles of 0.78, and 2.12 cm.
silica material are physically resistant, enable multiple functions,
present maximum levels of efficiency and are also compatible with Purity of silica
practically all solvents.
The responsibility for chromatographic separation of peptides and
Teknokroma has dedicated years of research and development in proteins is found inside the particle-within the pores. To obtain a
obtaining the best silica particle on the market. The silica particle on very homogeneous pore distribution the least possible number of
which the Europa columns is based is the result of an optimisation nanopores is essential.
process, starting with extremely pure materials with unusually low
metal content, and obtaining a perfectly spherical, rigid and inert For most reverse-phase silica packings, these nanopores are not
particle. properly chemically bonded, endcapped or deactivated. So when
nanopores are accessible to the peptides and proteins, surface-
peptide and protein interactions frequently dominate. These
interactions often result in a decrease of column efficiency.
210
Europa Protein C4 Pore Distribution Europa Protein C4 Phase Stability
Phase stability of Europa Protein C4 columns has been checked purging one 25 x 0.78
Europa Protein C 4 300 cm column either with CH3CN/1%TFA 10:90 (pH=1)during 15 hours at 0.9 ml/min or
with CH3CN/20 mM Na3PO4 10:90 (pH=12) during 3 hours at 1.7 ml/min.
Acid Resistance pH=1

2.0 100
High
1.5
90
dV/dD
(ml/g)
1.0
Retention to Initial (%)

80
0.5
70
0.0
100 200 300 400 500 600 700 800 900 1000
Pore Diameter (Å)
60
Deactivation Process
Low
Thanks to our propietary new Multifunctional Endcapping 50

Europa protein C4 Other Manufacture C4
Deactivation (MED) technology used with our popular HPLC
columns Mediterranea™ Sea 18, we obtain with the Europa
packing a specially designed C4, C8 and C18 ligand configuration,
Alkali Resistance pH=12
that blocks practically all the active centres that may have remained
1200
on the surface of the silica.
Low
As a result of this, Europa columns have an unusual low level of 1000
silanol activity, helping you to obtain symetrical peaks for the most
basic and acidic compounds. The improved high density bonding
Si Solved in Mobile Phase (ppm)
and full endcapping make them suitable to separate or purify low 800
molecular weight compounds (especially small peptides when
using Europa Peptide column 120 Å) and separate or purify high
molecular weight compounds, especially proteins when using 600
Europa Protein column 300 Å.

400
Europa C 18 bonding chemistries will help you to achieve an
extraordinary resistance and column lifetime when running at
extreme pH levels. 200
High
Wide pH Range
0
Europa protein C4 Other Manufacturer C4
Using Europa C 18 packing materials it is possible to work with
eluents from pH 1 to pH 12. Such unusual pH resistance values
have been achieved as a result of phase bonding efficiency and a
propietary endcapping process which provides a protective shield
against acidic and basic eluents.
Europa columns ensure greater separation efficiency, resistance to
extreme pH conditions and can be used for an extended period of
time.
211
Durability comparison in Alkaline Medium/RT Europa C18 Peptide HPLC columns
The graphic bellow shows the durability of the column after more We invite you to try our Europa C18 peptide column when you
than 80 hours of purge time passing through one Europa Protein experience unsatisfactory results with your favorite column.
C4 column a flow rate of 1.0 ml of alkaline solution at pH 12,
CH3CN/0.01NaOH 10/90 . Europa C18 Peptide columns are suitable to separate or purify low
There is represented in the graphic the retention time of naftalene after every three molecular weight compounds, especially small peptides.
hours of purge, using CH3CN / H2O 35:65 at 1.7 ml/min and 40ºC (UV detection at 254
nm). It is seen that after 80 hours, Europa columns still perform very well. Europa HPLC columns for peptides provide a high performance
110 that is unsurpassed in efficiency, reliability and reproducibility.
Manufactured using novel proprietary technologies, analytical
100
Europa protein C4 and preparative Europa columns are simply the best reverse
phase columns available today. Europa columns ensure greater
separation efficiency, resistance to extreme pH conditions and
90
Retention to Initial (%)
80
longer column life.
70 Our “Ultra-Fast” columns are made in 3-5 cm length in order to get

Other quick analytical results, whereas the “High Efficiency” columns
60 are normally in 15-25cm lengths to obtain the best resolution.
50
0 10 20 30 40 50 60 70 80 Specifications:
purge time (hours)
• Ultra high purity, totally spherical silica gel
Durability comparison in Acidic Medium / K value • High density bonding for extreme performance proprietary
fully end-capped silica
Europa C4 300A Other Manufacturer C4 300A • Porous Size: 120 Å, narrow particle size distribution
100 • Surface Area 300 m2/g
• % of Carbon 19 %
80 • High loading capacity of crude peptides
• Stable under basic and extreme acidic conditions
K value to Initial (%)
60 • Packed with 5µm sized silica particles
40 Microbore Columns are available in: 0.21, 0.30 cm ID

Analytical Columns are available in: 0.40 and 0.46 cm ID
20 Semi-Prep Columns are available in: 0.78 and 1.0 cm
Prep Columns are available in: 2.1 cm ID
0 Larger diameter available by request
0 3 6 9 12 15 18 21 24
purge time (hours)
Influence of Pore size in Peak Shape
Durability of Europa C4 has also been compare against other manufacturers using
a 15 x 0.46 cm column and CH3CN / 1.0% TFA in water 10:90 (pH=1) at 70ºC, and
checking K values for naftalene every 3 hours.
Protein Standards
1. Ribonuclease A (M.W. 13,700)
Tracer Excel C4 120A
Durability under Acidic Contition 2. Cytochrome C (M.W. 12,400)
3. Lysozyme (M.W. 14,300)
100
4. BSA (M.W. 67,000)
5. Myoglobin (M.W. 18,800)
6. Ovalbumin (M.W. 45,300)
Retention of Naphthalene to Initial (%)
80
60 Europa Protein C4 300A
40
20 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
Minutes
Column: 7.8 mm I.D. x 250 mm Length; Temperature: 35ºC; Detector: UV 220 nm;
0 Mobile Phase: A) CH3CN/H2O/TFA = 20/80/0.1, B) CH3CN/H2O/TFA = 60/40/0.1,
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 Linear Gradient from A to B in 25 min and hold for 10 min; Flow Rate: 1.7 ml/min.
Column Volumes
Retention time for naphtalene using the same chromatographic conditions has also
been controlled after up to 9000 column volumes of CH3CN / 0.05% TFA in water
(pH=2) at a flow rate of 1.0 ml/min at room temperature. Column size was 15 x 0.46 cm
212
Europa C18 Peptide Microbore HPLC Colums
Columns are particularly designed for LC/MS applications.

The high detection sensitivity of these columns allows the use of
smaller quantities of samples and also decreases the required
volume of solvents.
Length Diameter
Europa Peptide 120 C18 5 3 0.21 TR-010130
Europa packaging Europa Peptide 120 C18 5 25 0.30 TR-010140
Europa C18 Peptide Analytical HPLC Colums Europa C18 Peptide Semi Preparative HPLC Colums
Length Diameter
Packing Funct. µm cm cm Cat.Nbr. Length Diameter
Europa Peptide 120 C18 5 3 0.46 TR-010116 Packing Funct. µm cm cm Cat.Nbr.
Europa Peptide 120 C18 5 4 0.46 TR-010117 Europa Peptide 120 C18 5 10 0.78 TR-010141
Europa Peptide 120 C18 5 5 0.40 TR-010125 Europa C18 Peptide Preparative HPLC Colums
Length Diameter
213
Europa C18 Protein HPLC Columns Europa C18 Protein Analytical HPLC Colums
We invite you to try our Europa C18 Protein column when you
experience unsatisfactory results with your favorite column. Length Diameter
Europa C18 Protein columns are designed and manufactured for
Europa Protein 300 C18 5 3 0.46 TR-010158
identification and purification of proteins and for compounds with
Europa Protein 300 C18 5 4 0.46
high molecular weight. TR-010159
Europa HPLC columns for proteins provide a high performance that Europa Protein 300 C18 5 10 0.46 TR-010161
is unsurpassed in efficiency, reliability and reproducibility. Europa Protein 300 C18 5 15 0.46 TR-010162
Manufactured using novel proprietary technologies, analytical and Europa Protein 300 C18 5 20 0.46 TR-010163
preparative Europa columns are simply the best reverse phase Europa Protein 300 C18 5 25 0.46 TR-010164
columns available today. Europa Protein 300 C18 5 3 0.40 TR-010172
Europa columns ensure greater separation efficiency, resistance to Europa Protein 300 C18 5 4 0.40 TR-010173
extreme pH conditions and longer column life.
Our “Ultra-Fast” columns are made in 3-5 cm length in order TR-010175
to get quick analytical results, whereas the “High Efficiency” Europa Protein 300 C18 5 15 0.40 TR-010176
columns are normally in 15-25 cm lengths to obtain best resolution. Europa Protein 300 C18 5 20 0,40 TR-010177
Specifications:
• Ultra high purity totally spherical silica gel provide a high Europa C18 Protein Microbore HPLC Colums
resolution and excellent peak shape
• High loading capacity of crude proteins
• High density bonding for extreme performance proprietary Columns are particularly designed for LC/MS applications.
fully end-capped silica The high detection sensitivity of these columns allows the use of
• Stable, featuring extended acidic and basic conditions smaller quantities of samples and also decreases the required
• Silica properties: ultra pure and totally spherical narrow volume of solvents.
distribution range and high density Length Diameter
• Fully end-capped silica Packing Funct. µm cm cm Cat.Nbr.
• Porous Size: 300Å narrow particle size distribution
• Surface Area 100 m2/gr.
• % of Carbon 7 % TR-010185
• Packed with 5µm sized silica particles Europa Protein 300 C18 5 10 0.21 TR-010186
• Available as C4, C8, and C18 columns Europa Protein 300 C18 5 15 0.21 TR-010187
• Microbore Columns are available in: 0.21, 0.30 cm I.D. Europa Protein 300 C18 5 20 0.21 TR-010188
Analytical Columns in: 0.40 and 0.46cm I.D. Semi-Prep in: Europa Protein 300 C18 5 3 0.30 TR-010195
0.70-1.0cm Prep Columns in: 2.1cm and larger diameter Europa Protein 300 C18 5 5 0.30 TR-010196
by request Europa Protein 300 C18 5 10 0.30 TR-010197
Europa C18 Protein Preparative HPLC Colums Europa Protein 300 C18 5 20 0.30 TR-010199
Europa C18 Protein Semi-Preparative HPLC Colums

Length Diameter
Europa Protein 300 C18 5 5 2.12 TR-010217 Length D
iameter
Europa Protein 300 C18 5 10 2.12 TR-010218 Packing Funct. µm cm cm Cat.Nbr.
Europa Protein 300 C18 5 15 2.12 TR-010219 Europa Protein 300 C18 5 10 0.70 TR-010211
214
Semi preparative and Preparative Europa HPLC

Columns
Europa C8 Protein HPLC Columns Length D

iameter
Europa C8 columns are recommended for compounds too Europa Protein 300 C8 5 5 0.30 TR-010190
strongly retained on C18 Phases. Europa Protein 300 C8 5 10 0.30 TR-010191
Europa C8 Protein Analytical HPLC Colums Europa Protein 300 C8 5 20 0.30 TR-010193
Length Diameter Europa C8 Protein Semi-Preparative HPLC Colums

Europa Protein 300 C8 5 4 0.46 TR-010152 Length Diameter
Europa Protein 300 C8 5 10 0.40 TR-010168 Europa C8 Protein Preparative HPLC Colums
Europa Protein 300 C8 5 20 0,40 TR-010170
Europa C8 Protein Microbore HPLC Colums Length D

iameter
Columns are particularly designed for LC/MS applications. Europa Protein 300 C8 5 10 2.12 TR-010208
The high detection sensitivity of these columns allows the use of Europa Protein 300 C8 5 15 2.12 TR-010209
smaller quantities of samples and also decreases the required Europa Protein 300 C8 5 25 2.12 TR-010210
volume of solvents.
Europa C8 columns are recommended for For Guard Columns please refer to pages 193-196
compounds too strongly retained on C18 Phases.
Length Diameter
215
Europa C4 Protein HPLC Columns Length D
iameter
Europa Protein C4 300 Å - Loading Capacity of BSA Europa Protein 300 C4 5 3 0.40 TR-010088
Protein 300 exhibited the highest loading capacity for proteins Europa Protein 300 C4 5 4 0.40 TR-010089
Tracer Excel C4 120 A
Europa Protein Europa Protein 300 C4 5 15 0.40 TR-010092
UV abrorb

Break through point
Europa C4 Protein Microbore HPLC Colums
Columns are particularly designed for LC/MS applications.

0 20 40 60 80 100 The high detection sensitivity of these columns allows the use of
time (minutes) smaller quantities of samples and also decreases the required
volume of solvents.
Column: 7 mm I.D. x 250 mm Length; Temperature: 35ºC; Detector: UV 220 nm;
Flow Rate: 1.0 ml/min.
Length Diameter
Feed: 10 mg/mL BSA in 0.1% TFAaq Packing Funct. µm cm cm Cat.Nbr.
Europa C4 columns are recommended for compounds too strongly
retained on C 18 and C 8
Europa Protein C4 300 Å - Protein Separation Behaviors Europa Protein 300 C4 5 10 0.21 TR-010097
• Similar Hydrophobic Selectivity Protein Standards

• Higher Resolution 1. Ribonuclease A (M.W. 13,700) Europa Protein 300 C4 5 20 0.21 TR-010099
2. Cytochrome C (M.W. 12,400)
3. Lysozyme (M.W. 14,300) Europa Protein 300 C4 5 3 0.30 TR-010100
4. BSA (M.W. 67,000)
5. Myoglobin (M.W. 18,800)
6. Ovalbumin (M.W. 45,300) Europa Protein 300 C4 5 10 0.30 TR-010102
Tracer Excel 300 C4 Europa Protein 300 C4 5 15 0.30 TR-010103

3 4
Conventional 1 2 6 Europa Protein 300 C4 5 20 0.30 TR-010104
5
Europa C4 Protein Semi-Preparative HPLC Colums
2 time3(minutes)
0 5 10 1 15 20Impurities
25 30 35
Europa Protein C4 Retention
4 6
Length D
iameter
5 Packing Funct. µm cm cm Cat.Nbr.
0 5 10 15 20 25 30 35
Retention time (minutes) TR-010108
Colum: 6 mm I.D. x 250 mm Length; Temperature: 35ºC; Detector: UV 220 nm; Europa Protein 300 C4 5 10 1.00 TR-010109
Mobile Phase: A) CH3CN/H2O/TFA = 20/80/0.1, B) CH3CN/H2O/TFA = 60/40/0.1, Europa Protein 300 C4 5 15 1.00 TR-010110
Linear Gradient from A to B in 25 min and hold for 10 min; Flow Rate: 1.7 ml/min.
Europa C4 Protein Analytical HPLC Colums
Europa C4 Protein Preparative HPLC Colums
Length D
iameter
Europa Protein 300 C4 5 3 0.46 TR-010081 Length Diameter
216
Tracer Excel™
TRACER EXCEL™ is a range of totally new packings that Total deactivation

employ the most advanced procedures of synthesis and chemical
functionalization, resulting in some column packings that completely Free surface silanols that are left exposed following functional
surpass other silica-based packings on the market. bonding of the silica particle are the chief cause of peak tailing and
distortion that commonly appear with basic compounds.
To manufacture the silica particle, the basis of all TRACER EXCEL
packings, we begin with materials of extreme purity and follow If the silica particle also contains significant quantities of metals,
strictly controlled processes. In this way, we get a totally porous, these markedly increase the acidity of these surface silanols,
spherically perfect particle, without surface irregularities and with keeping them ionized even at low pHs. These conditions can
an extremely low content of metals (Al, Fe, Ti and Zn). cause deleterious effects on eluting chromatographic peaks.
The rigorous control of the process variables also allows us The Pyridine/Phenol test is an excellent marker of the presence of
to obtain a material with a perfectly reproducible porosity and these surface silanols. Under ideal conditions, the pyridine peak
surface area, and with a practical absence of micropores. In other should elute before the phenol peak and should also elute with
competitors’ packings, these micropores cause chromatographic total symmetry without tailing. Furthermore, a broader separation
problems due to incomplete substitution of the support, while with between the two peaks indicates superior deactivation.
TRACER EXCEL packings micropores are totally eliminated.
The TRACER EXCEL ODS-A column complies with the pyridine/
We are therefore able to offer you a complete line of HPLC phenol test better than other columns from major manufacturers.
packings with characteristics of reproducibility, purity, deactivation, This demonstrates the extraordinary deactivation achieved with
fluido-dynamic behaviour and chemical and physical stability that TRACER EXCEL ODS-A columns. Another test that demonstrates
are difficult to beat. the quality of TRACER EXCEL ODS-A columns is the acidic
compounds test. This type of compound yields evidence of the
• Exceptional batch-to-batch reproducibility. presence of chelating centres or points of ionic interchange that
• Ultra-pure silica. may be present in the silica particle.
• Extremely low content of metals.
• Perfect sphericity.
• Meticulously controlled materials.
• Maximum pH range (between 1.5 and 11.0)
• 3, 5 and 10 µm particles Pyridine/Phenol test
• Easily scaled-up, from microbore to preparative HPLC.
• Available with 300A pore size for biochromatography.
• Exceptional long lifetime.
• Wide range of packings.
• Fully deactivated after functional bonding.
TRACER EXCEL ODS-A

TRACER EXCEL ODS-A is a totally endcapped packing, notable
for its extreme level of deactivation. This minimizes undesirable
interactions when chromatographing strongly acidic or basic
analytes or chelating compounds.
Additionally TRACER EXCEL ODS-A columns show extraordinary

resistance to extreme pH values, between 1.5 to 11.0.
Maximum Stability
Conditions of test Composition:
The chemical and structural stability of TRACER EXCEL columns leads to
Eluant : Acetonitrile/Water, 30/70 1ml/min Pyridine 2.1µl/ml
long useful lifetimes, even under extreme conditions where columns of most Lambda: 265nm Phenol:14 mg/m
major manufacturers would suffer rapid degradation.
217
Tracer Excel™
TRACER EXCEL columns show perfectly symmetrical peaks in Purity of material
contrast to the significant tailing which appears when this test is
done with other columns on the market. Symmetrical peaks are All of the advantages of TRACER EXCEL columns have as a base
achieved even when separating basic compounds. the quality of the silica particle. No bonding process can mask
silica of inferior quality. Only silica particles absolutely free of
Once again, TRACER EXCEL columns show, thanks to their metallic impurities, with a pore-size and pore-distribution absolutely
exceptional level of deactivation, excellence in obtaining perfectly controlled and synthesized through fully optimized processes, can
symmetrical peaks where other columns on the market clearly fail give bonded packings of the highest grade.
(giving peaks with pronounced tails or even irreversible adsorption).
The 8-quinolinol/acetylacetone test demonstrates the difference in
chromatographic behavior between TRACER EXCEL ODS-A and
a competitor’s column with a high content of metallic impurities for
Acid Compounds Test
the chelating compound 8-quinolinol.
Metalic Trace Test
Eluant : 20 mM KH2PO4pH3.2/CH3CN 65:35 Uracil: 0.5mg/ml

1 ml/min. Temp 40ºC UV 245nm Benzoic acid: 3.6 mg/ml
p-Ethylbenzoic acid: 0.9 mg/ml
Methylbenzene:3.0 mg/ml
Tracer Excel ODS-A 8-Quinolinol: 0.5mg/ml

Eluant : 10 mM KH2PO4pH6.8/Metanol 60:40 Actetylacetone: 0.5mg/ml
Basic Compounds Test 1 ml/min. Temp 30ºC UV 254nm
Tracer Excel ODS-A

Reproductibility
The high productivity which is now needed in analytical and governmental
laboratories oblige everyone to use reliable HPLC equipment and reprodu-
cible columns.
TRACER EXCEL columns were developed with the final objective of achieving
the very highest quality and reproducibility. Teknokroma’s numerous and
stringent process controls for every batch of packing fully guarantees high
Spherisorb ODS2 quality and exceptional reproducibility.
Tracer Excel ODS-A Propanolol: 0.08mg/ml

Eluant : 20 mM KH2PO4pH7/CH2CN 35:65 Diphenidramine :1.28 mg/ml
1 ml/min. Temp 25ºC UV 235nm Acetonaphthalene: 0.2 mg/ml
Amyltryptilene: 0.3 mg/ml
218
Tracer Excel ODS-B
TRACER EXCEL ODS-B Generally, its field of application is the same as that of the TRACER
EXCEL ODS-A, but its field of application is extended for samples
• Compatible with 100% aqueous eluent. which are especially difficult for conventional reversed phases, as is
• Especially suitable for the separation of hydrophilic compounds. the case in separating oligosaccharides, amino acids, nucleotides
• Strong retention in aqueous eluents. and organic acids.
• Long useful life with aqueous eluents
• Selectivity complementary to TRACER EXCEL ODS-A The special chromatographic conditions of TRACER EXCEL
• High mechanical stability ODS-B also provide a specific selectivity for compounds which
• Maximum versatility. contain slightly polar groups in their structure.
This column is especially recommended for LC-MS in that, in many
Based on the same principles as the TRACER EXCEL ODS-A cases, the use of plugs or ionic blocking agents are avoided, which
columns, the TRACER EXCEL ODS-B column presents a high negatively affect detection when this technique is used.
selectivity for hydrophilic and polar compounds, which are poorly As shown in the chromatogram, after more than 100 hours of
retained on conventional ODS columns. operations with water no alteration is observed in retention times,
selectivity or distortion in the peaks of pyridine and phenol - a
A special modification in the process of functionalizing the pure clear indication that no collapse of the bonded phase functionality
silica particle prevents the collapsing effect of the C18 chains is adversely achieved with TRACER EXCEL ODS-B columns.
when working with mainly aqueous eluants. So you can work with Interestingly, the collapsing of bonded phase functionality with the
excellent chromatographic performance even when the percentage majority of reversed phase columns on the market is typical under
of the aqueous phase is 100%. these conditions.
EFFECT OF AQUEOUS ELUTANTS ON THE ORGANIZATION OF

HYDROCARBON CHAINS.
Tracer Excel ODS-B ODS Conventional Inicial Chromatogram After 100 hours
TMS endcap washed with H2O
Pyridine Pyridine
Phenol
Water Phenol
Silica Water Silica
ODS
ODS TMS endcap
K´ 18
Reproductibility Tracer Excel ODS-B
16
14
12
10
0 1 2 3 4
Lotes
219
Tracer Excel ODS-B
Antioxidants Water Soluble Vitamins
Column: TRACER EXCEL ODS-B 5 µm, 15 x 0,46 cm Column: TRACER EXCEL ODS-B 5 µm, 15 x 0,46 cm
Eluant: Phosphate Plug 0,1 M (pH 3,1) Eluant: Phosphate Plug 20 mM (pH 70)CH3CN 95/5 cm
Flow: 0,6 ml/min. Flow: 0.6 ml/min.
Detector: ECD Detector: UV 210 nm
Sample: 1 Ascorbic Acid Sample: 1 Calcium Pantothenate

2 GSH 2 Pyridoxine hydrochloride (B6)
3 Nicotinamide
Glycolid Acid and Latic Acid Alcohols
Column: TRACER EXCEL ODS-B 5 µm, 15 x 0.46 cm Column: TRACER EXCEL ODS-B 5 µm, 15 x 0,46 cm
Eluant: H3PO4 0.1% Eluant: H2O
Flow: 0.6 ml/min. Flow: 0,6 ml/min.
Temperature: 40ºC Temperature: 40ºC
Detector: UV 210 nm Detector: RID
Sample: 1 Glycolic Acid Sample: 1 Methanol

2 Lactic Acid 2 Ethanol
3 Iso-Propanol
4 n-propanol
Aminoacids Cyclodextrin derivatives
Column: TRACER EXCEL ODS-B 5 µm, 15 x 0.46 cm Column: TRACER EXCEL ODS-B 5 µm, 15 x 0.46 cm
Eluant: H2O Eluant: MeOH/H2O 70:30
Flow: 0.6 ml/min. Flow: 0.6 ml/min.
Temperature: 40ºC Temperature: ambient
Detector: RID Detector: UV240 nm
Sample: 1 Alanine Sample: 1 61, 65 -di-O-trytyl-cG8

2 Valine 2 61, 64 -di-O-trytyl-cG8
3 Isoleucine 3 61, 63 -di-O-trytyl-cG8
4 Leucine 4 61, 62 -di-O-trytyl-cG8
Ascorbic Acid and Glycosides Fructo-oligosccharides
Column: TRACER EXCEL ODS-B 5 µm, 15 x 0.46 cm Column: TRACER EXCEL ODS-B 5 µm, 15 x 0,46 cm
Eluant: Phosphate Plug (pH 3,8) Eluant: H2O
Flow: 04 ml/min. Flow: 06 ml/min.
Temperature: Ambient Temperature: Ambient
Detector:UV240 nm Detector:RID
220
Other Tracer Excel Packings
The extraordinary quality of TRACER EXCEL packings have been extended to a full range of operations,covering practically
all the chromatographer’s needs.
Si Material of the ultrapure silica particle, the basis of all the TRACER EXCEL range.
C8 This packing made operative with octyl groups and totally endcapped is extremely versatile.
Its use is recommended for highly hydrophobic samples, which are retained excessively on ODS type packings.
Developed on the same ultrapure silica as ODS-A and ODS-B,it is extremely reproducible and reliable.
C4 The same ultra pure silica of all the TRACER EXCEL range made operative with butyl groups,giving a moderately
hydrophobic
packing.
Its principle field of application is the separation of peptides and proteins by reverse phase.
In this case,the same packing is used with a 300 A porosity,more suitable for the large size of protein molecules.
Another field where this packing can be highly recommended is when the sample contains compounds of a very
different hydrophobic nature.
This packing permits perfect separation of a sample with a single injection.

C1 The same ultrapure silica of the TRACER EXCEL range is given its special function with tri-methylchlorosilane to create
a low hydrophobic reversed phase.
Its field of application includes the separation of peptides and proteins by reversed phase.
It can also be used as a packing for normal phase with highly polar compounds.
CN The type CN packings are much appreciated as alternatives to ODS-type packings for their special selectivity , as well
as for the possibility they offer for working in both chromatographic modes,normal and reverse phase.
However, in comparison with the latter,they have always been characterised by a lesser reproducibility and a notably
shorter useful life.
Thanks to the extraordinary level of quality of the silica of the particle and the optimization reached by the actuating
processes, the new packing TRACER EXCEL 120 CN has satisfactorily overcome these limitations, so giving the
chromatogapher a completely reliable alternative.
As a normal phase it is an excellent alternative to unsubstituted silica, given that retention times are much more
reproducible, equilibration times much more rapid, and it does not suffer the problems of de-activation of silica itself.
NH2 This packing, with chemically bonded groups of aminopropyl silane, can be used as a phase normal or reverse phase
packing depending on the eluant used.
It is recommended for separations of basic compounds under normal phase conditions.
Additionally, the reactivity of the amino group makes it very suitable as a support for later modifications as for example
in the synthesis of quiral phases.
It is also very suitable for SFC applications

Ph In the same way as the CN type packing, the packing substituted with dimethyl phenyl can be used in normal or
reversed phase, being in this latter case a very useful alternative to ODS type packings since its aromatic groups give it
a special selectivity when polar compounds are being chromatographed.
300 A complete range of packings with a pore diameter of 300 Angstrom units is available, ideal for undertaking separations
Angstrom of complex molecules of very high molecular weight, e.g. proteins and peptides.
221
General Properties of Tracer Excel Packings
ODS-A ODS-B C8 C4 C1 CN Ph NH2 SI
Size of pore
120 120 120 120 120 120 120 120 120
in A units
Size of
3, 4, 5 & 10 µm 3, 4, 5 & 10 µm 3, 5 and 10 µm 3, 5 and 10 µm 3, 5 and 10 µm 3, 5 and 10 µm 3, 5 and 10 µm 3, 5 and 10 µm 3, 5 and 10 µm
particle
Volume of
pores in 1.0 ml/g 1.0 ml/g 1.0 ml/g 1.0 ml/g 1.0 ml/g 1.0 ml/g 1.0 ml/g 1.0 ml/g 1.0 ml/g
ml/g
Surface
300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g
area
Purity of
Ultrapure Ultrapure Ultrapure Ultrapure Ultrapure Ultrapure Ultrapure Ultrapure Ultrapure
silica
%C 17% 15% 10% 8% 5% 7% 9% 4%
Monofunctional Monofunctional Monofunctional Monofunctional Monofunctional
Type of
and totally and totally and totally and totally Monofunctional and totally Trifunctional
phase
endcapped endcapped endcapped endcapped endcapped
Metallic
Less than Less than Less than Less than Less than Less than Less than Less than Less than
impurities
10ppm of each 10ppm of each 10ppm of each 10ppm of each 10ppm of each 10ppm of each 10ppm of each 10ppm of each 10ppm of each
(Al, Fe, Ti,
one one one one one one one one one
Zr)
USP L1 L1 L7 L26 L13 L10 L11 L8 L3
222
Tracer Excel 120
Analytical columns 0.4 cm I.D.
TRACER EXCEL 120/5 µm
L e n g t h c m
Function µm 4 cm 5 cm 10 cm 15 cm 20 cm 25 cm
ODS-A 5 TR-416336 TR-417000 TR-416337 TR-416338 TR-416339 TR-416340
ODS-B 5 TR-416341 TR-417002 TR-416342 TR-416343 TR-416344 TR-416345
Si 5 TR-416356 TR-417004 TR-416357 TR-416358 TR-416359 TR-416360
C8 5 TR-416361 TR-417006 TR-416362 TR-416363 TR-416364 TR-416365
C4 5 TR-416366 TR-417008 TR-416367 TR-416368 TR-416369 TR-416370
C1 5 TR-416371 TR-417010 TR-416372 TR-416373 TR-416374 TR-416375
NH2 5 TR-416376 TR-417012 TR-416377 TR-416378 TR-416379 TR-416380
CN 5 TR-416381 TR-417014 TR-416382 TR-416383 TR-416384 TR-416385
Ph 5 TR-416386 TR-417016 TR-416387 TR-416388 TR-416389 TR-416390
Ultrarapid columns 0.4 cm I.D.

L e n g t h c m
Si 3 TR-413470 TR-417022 TR-413471 TR-413472 TR-413473 TR-413474
C8 3 TR-413475 TR-417024 TR-413476 TR-413477 TR-413478 TR-413479
C4 3 TR-413480 TR-417026 TR-413481 TR-413482 TR-413483 TR-413484
C1 3 TR-413485 TR-417028 TR-413486 TR-413487 TR-413488 TR-413489
NH2 3 TR-413490 TR-417030 TR-413491 TR-413492 TR-413493 TR-413494
CN 3 TR-413495 TR-417032 TR-413496 TR-413497 TR-413498 TR-413499
Ph 3 TR-413500 TR-417034 TR-413501 TR-413502 TR-413503 TR-413504

L e n g t h c m
Si 5 TR-016356 TR-025004 TR-016357 TR-016358 TR-016359 TR-016360
C8 5 TR-016361 TR-025006 TR-016362 TR-016363 TR-016364 TR-016365
C4 5 TR-016366 TR-025008 TR-016367 TR-016368 TR-016369 TR-016370
C1 5 TR-016371 TR-025010 TR-016372 TR-016373 TR-016374 TR-016375
NH2 5 TR-016376 TR-025012 TR-016377 TR-016378 TR-016379 TR-016380
CN 5 TR-016381 TR-025014 TR-016382 TR-016383 TR-016384 TR-016385
Ph 5 TR-016386 TR-025016 TR-016387 TR-016388 TR-016389 TR-016390

L e n g t h c m

L e n g t h c m
223
Tracer Excel 120
L e n g t h c m
Si 3 TR-013425 TR-025038 TR-013426 TR-013427 TR-013428 TR-013429
C8 3 TR-013430 TR-025040 TR-013431 TR-013432 TR-013433 TR-013434
C4 3 TR-013435 TR-025042 TR-013436 TR-013437 TR-013438 TR-013439
C1 3 TR-013440 TR-025044 TR-013441 TR-013442 TR-013443 TR-013444
NH2 3 TR-013445 TR-025046 TR-013446 TR-013447 TR-013448 TR-013449
CN 3 TR-013450 TR-025048 TR-013451 TR-013452 TR-013453 TR-013454
Ph 3 TR-013455 TR-025050 TR-013456 TR-013457 TR-013458 TR-013459
Microbore columns 0.21 cm I.D.

L e n g t h c m
Function µm 5 cm 10 cm 15 cm 20 cm 25 cm
ODS-B 5 TR-025060 TR-021353 TR-025078 TR-021354 TR-025096
Si 5 TR-025062 TR-021395 TR-025080 TR-021364 TR-025098
C8 5 TR-025064 TR-021365 TR-025082 TR-021366 TR-025100
C4 5 TR-025066 TR-021367 TR-025084 TR-021368 TR-025102
C1 5 TR-025068 TR-021369 TR-025086 TR-021370 TR-025104
NH2 5 TR-025070 TR-021371 TR-025088 TR-021372 TR-025106
CN 5 TR-025072 TR-021373 TR-025090 TR-021374 TR-025108
Ph 5 TR-025074 TR-021375 TR-025092 TR-021376 TR-025110
Other configurations available on demand

L e n g t h c m
ODS-A 3 TR-025114 TR-021407 TR-025134 TR-021408 TR-025154
ODS-B 3 TR-025116 TR-021409 TR-025136 TR-021410 TR-025156
Si 3 TR-025118 TR-021411 TR-025138 TR-021412 TR-025158
C8 3 TR-025120 TR-021413 TR-025140 TR-021414 TR-025160
C4 3 TR-025122 TR-021415 TR-025142 TR-021416 TR-025162
C1 3 TR-025124 TR-021417 TR-025144 TR-021418 TR-025164
NH2 3 TR-025126 TR-021419 TR-025146 TR-021420 TR-025166
CN 3 TR-025128 TR-021421 TR-025148 TR-021422 TR-025168
Ph 3 TR-025130 TR-021423 TR-025150 TR-021424 TR-025170
224
Tracer Excel 120
L e n g t h c m
Function µm 5 cm 10 cm 15 cm 20 cm
ODS-A 5 TR-025200 TR-021355 TR-025220 TR-021356
ODS-B 5 TR-025202 TR-021357 TR-025222 TR-021358
Si 5 TR-025204 TR-021381 TR-025224 TR-021382
C8 5 TR-025206 TR-021383 TR-025226 TR-021384
C4 5 TR-025208 TR-021385 TR-025228 TR-021386
C1 5 TR-025210 TR-021387 TR-025230 TR-021388
NH2 5 TR-025212 TR-021389 TR-025232 TR-021390
CN 5 TR-025214 TR-021391 TR-025234 TR-021392
Ph 5 TR-025216 TR-021393 TR-025236 TR-021394

L e n g t h c m
ODS-A 3 TR-025240 TR-021425 TR-025260 TR-021426
ODS-B 3 TR-025242 TR-021427 TR-025262 TR-021428
Si 3 TR-025244 TR-021429 TR-025264 TR-021430
C8 3 TR-025246 TR-021431 TR-025266 TR-021432
C4 3 TR-025248 TR-021433 TR-025268 TR-021434
C1 3 TR-025250 TR-021435 TR-025270 TR-021436
NH2 3 TR-025252 TR-021437 TR-025272 TR-021438
CN 3 TR-025254 TR-021439 TR-025274 TR-021440
Ph 3 TR-025256 TR021441 TR-025276 TR-021442
225
Tracer Excel 120
Semi-preparative columns 0.78 cm I.D.
L e n g t h c m
ODS-A 5 TR-025280 TR-025300 TR-016167 TR-016168
ODS-B 5 TR-025282 TR-025302 TR-016171 TR-016172
Si 5 TR-025284 TR-025304 TR-016175 TR-016176
C8 5 TR-025286 TR-025306 TR-016179 TR-016180
C4 5 TR-025288 TR-025308 TR-016183 TR-016184
C1 5 TR-025290 TR-025310 TR-016187 TR-016188
NH2 5 TR-025292 TR-025312 TR-016191 TR-016192
CN 5 TR-025294 TR-025314 TR-016195 TR-016196
Ph 5 TR-025296 TR-025316 TR-016199 TR-016200
Semi-preparative columns 1.0 cm I.D.

L e n g t h c m
ODS-A 5 TR-025320 TR-025340 TR-016169 TR-016170
ODS-B 5 TR-025322 TR-025342 TR-016173 TR-016174
Si 5 TR-025324 TR-025344 TR-016177 TR-016178
C8 5 TR-025326 TR-025346 TR-016181 TR-016182
C4 5 TR-025328 TR-025348 TR-016185 TR-016186
C1 5 TR-025330 TR-025350 TR-016189 TR-016190
NH2 5 TR-025332 TR-025352 TR-016193 TR-016194
CN 5 TR-025334 TR-025354 TR-016197 TR-016198
Ph 5 TR-025336 TR-025356 TR-016201 TR-016202
226
Tracer Excel 300
L e n g t h c m
C8 5 TR-016405 TR-025362 TR-016406 TR-016407 TR-016408 TR-016409
C4 5 TR-016410 TR-025364 TR-016411 TR-016412 TR-016413 TR-016414


L e n g t h c m
C8 5 TR-416405 TR-417042 TR-416406 TR-416407 TR-416408 TR-416409
C4 5 TR-416410 TR-417044 TR-416411 TR-416412 TR-416413 TR-416414

L e n g t h c m
ODS-A 5 TR-025376 TR-012395 TR-025382 TR-012396 TR-025388
C8 5 TR-025378 TR-012397 TR-025384 TR-012398 TR-025390
C4 5 TR-025380 TR-012399 TR-025386 TR-012400 TR-025392


L e n g t h c m
ODS-A 5 TR-025396 TR-021401 TR-025402 TR-021402 TR-025408
C8 5 TR-025398 TR-021403 TR-025404 TR-021404 TR-025410
C4 5 TR-025400 TR-021405 TR-025406 TR-021406 TR-025412
For Guard Columns please refer to pages 193-196
227
Tracer Extrasil
Tracer Extrasil Volume %
The new range of Tracer Extrasil packings has been specially

developed to replace one of the most popular packings on the
market (WS).
All the physical and chromatographic parameters evaluated show
a total equivalence between both materials, and what is more
important, this has been certified by the excellent results obtained
by the many users who upto now have tried this packing. Particle Diameter (µm)
Economy
Another Packing Volume %
Tracer Extrasil represents the most economical choice of HPLC
packings.
Reproducibility
An advanced manufacturing process and a strict control of each
one of its steps ensures a maximum reproducibility and efficiency
in every one of the columns.
Particle Diameter (µm) TKL 10015
Guarantee
S.E.M. of the silica particle
The confidence we have in our product enables us to offer a
complete guarantee on these columns, so that if for any reason The packing that results shows an almost perfect sphericity, as the
whatever a client thinks that a TRACER EXTRASIL column does images made by a scanning electron microscope show.
not operate in an identical manner to the equivalent WS packing,
we will refund his money.
Tracer EXTRASIL
Characteristics of the material
As shown in the following table,the new packing TRACER
EXTRASIL is perfectly equivalent to the reference material in all its
physicochemical characteristics.
Characteristics
Tracer Extrasil Particle Size WS Packing
3,5 & 10 µm Pore Size 3,5 & 10 µm
80 A Surface area 80 A
220 m2/g Carbon content 220 m2/g
4% C1 4%
6% C6 6%
Another Packing
6% C8 6%
7% ODS-1 7%
12% ODS-2 12%
3,5% CN 3,5%
2% NH2 2%
3,0% Phenyl 3,0%
- SAX -
- SCX -
Distribution of particle size

In the development of this new material there has been special
care in optimization of the size of the particle, given that this control
is essential to get the best efficiency and stability in the packing.
Applications
The comparison made with the WS packing shows once more the
In addition to the complete agreement between the comparative
total equivalence of these two materials.
data for both packings, the definitive proof comes from their
comparison in a wide range of applications.
228
Tracer Extrasil
Catecholamines Tracer Extrasil WS Packing
Dimensions: 250 x 4.6 mm
Mobil Phase: CH2OH:25 mM KH2PO4 pH 2.0 (2:98)
Flow Rate: 1.0mL/min
Temperature: 40°C
Detection: UV@ 270nm
Sample: 1. Norepinephrine
2. Betametasone
3. Dopamine
4. L-DOPA
5. Serotonine
5µ C6 5µ C6
Nucleotides
Mobil Phase: A: 0.04M KH2PO4 pH 5.5
B: 0.5M KH2PO4TpH 5.5
Sample: 1. β-NAD
2. IMP
3. GMP
4. AMP
5. GDP
6. ADP
7. NADP 5µ SAX 5µ SAX
8. ITP
9. ATP
Corticosteroids
Mobil Phase: CH2Cl2:CH3OH (95:5)
Sample: 1. Deoxicorticosterone Acetate
2. Desoxicorticosterone
3. Hidrocortisone 21-Acetate
4. Corticosterone
5. Cortisone
6. Hidrocortisone 5µ NH2 5µ NH2
Aromatic Ketones
Mobil Phase: CH2 CN :CH2 O (33:67)
Sample: 1. Benzamide
2. Alcohol Bencilic
3. Acetophenone
4. Methyl Benzoat
5. Phenetole
6. Naphtalene
7. Benzophenone
8. Biphenile 5µ Phenyl 5µ Phenyl
229
Tracer Extrasil
SRM 869 Tracer Extrasil WS Packing
Mobil Phase: H2 O:CH3CN (15:85)
Temperature: 35°C
Sample: 1. Benzo (a) pirene (BaP)
2. Phenantro (3,4-C)
2. Phenantrene (Ph Ph)
3. Tetrabenzonaphtalene
Tracer Extrasil ODS 2 aTBN/BaP = 1,77
Packing WS ODS-2 aTBN/BaP = 1,70 5µ ODS(1) 5µ ODS(1)
4-Hydroxybenzoates
Sample: 1. Methyl-4-hidroxibenzoate
2. Ethyl-4-hidroxibenzoate
3. Propyl-4-hidroxibenzoate
4. Butyl-4-hidroxibenzoate
5µ ODS(1) 5µ ODS(1)
4-Hydroxybenzoates
Sample: 1. Methyl- 4-hidroxibenzoate
2. Ethyl-4-hidroxibenzoate
3. Propyl-4-hidroxibenzoate
4. Butyl-4-hidroxibenzoate
5µ C8 5µ C8
Hydrosoluble Vitamines
Mobil Phase: A: 5mM 1-Penta sodic nesulfonate in 0.1%
H3PO4
B: 5mM 1-Sodic Pentanesulfonate in 0.1%
H3PO4 in 80 % CH3CN A:B (97.5:2.5) to A:B
(70:30) in 20 min.
Sample: 1. Nicotinamine
2. Pyridoxal
3. Acide p-amynobenzaic
4. Tyamine
5µ C8 5µ C8
5. Folic Acid
6. Riboflavine
230
Tracer Extrasil
4-Hydroxybenzoat Tracer Extrasil WS Packing
Temperature: 40°C
Sample: 1. Methyl-4-hidroxibenzoat
2. Ethyl-4-hidroxibenzoat
3. Propyl-4-hidroxibenzoat
4. Butyl-4-hidroxibenzoat
Polar Compounds
Mobil Phase: 25mM KH2PO4,pH 2.5
Temperature: 40°C
Sample: 1. L-Cisteine
2. L-ascorbic Acid
3. Glutatione
4. Uric Acid
Liposoluble Vitamines
Mobil Phase: CH3CN:CH3OH (75:25)
Sample: 1. Vitamine A
2. Vitamine A Acetate
3. Vitamine D2
4. Vitamine D3
5. Vitamine E
6. Vitamine E Acetate
7. Vitamine K1 5µ ODS(2) 5µ ODS(2)
Pesticides/Herbicides
Sample: 1. Baygon™
2. Carbofuran
3. Carbanyl
4. Propham
5. Captan
231
Tracer Extrasil
Analytical columns Tracer EXTRASIL
F u n c t i o n D i m e n s i o n s
Particle size (µm) 10 x 0.46 cm 10 x 0.4 cm 12.5 x 0.46 cm 12.5 x 0.4 cm 15 x 0.46 cm 15 x 0.4 cm 20 x 0.46 cm 20 x 0,4 cm 25 x 0.46 cm 25 x 0.4 cm
ODS1 5 TR-016050 TR-416050 TR-016051 TR-416051 TR-016052 TR-416052 TR-016053 TR-416053 TR-016054 TR-416054
Si 5 TR-016060 TR-416060 TR-016061 TR-416061 TR-016062 TR-416062 TR-016063 TR-416063 TR-016064 TR-416064
C1 5 TR-016065 TR-416065 TR-016066 TR-416066 TR-016067 TR-416067 TR-016068 TR-416068 TR-016069 TR-416069
CN 5 TR-016080 TR-416080 TR-016081 TR-416081 TR-016082 TR-416082 TR-016083 TR-416083 TR-016084 TR-416084
NH2 5 TR-016085 TR-416085 TR-016086 TR-416086 TR-016087 TR-416087 TR-016088 TR-416088 TR-016089 TR-416089
Phenyl 5 TR-016090 TR-416090 TR-016091 TR-416091 TR-016092 TR-416092 TR-016093 TR-416093 TR-016094 TR-416094
SAX 5 TR-016095 TR-416095 TR-016096 TR-416096 TR-016097 TR-416097 TR-016098 TR-416098 TR-016099 TR-416099
SCX 5 TR-016100 TR-416100 TR-016101 TR-416101 TR-016102 TR-416102 TR-016103 TR-416103 TR-016104 TR-416104
Si 10 TR-016115 TR-416115 TR-016116 TR-416116 TR-016117 TR-416117 TR-016118 TR-416118 TR-016119 TR-416119
NH2 10 TR-016135 TR-416135 TR-016136 TR-416136 TR-016137 TR-416137 TR-016138 TR-416138 TR-016139 TR-416139
SAX 10 TR-016151 TR-416151 TR-016152 TR-416152 TR-016153 TR-416153 TR-016154 TR-416154 TR-016155 TR-416155
Ultrarapid columns Tracer EXTRASIL
Particle size (µm) 4 x 0.46 cm 4 x 0.4 cm 5 x 0.46 cm 5 x 0.4 cm 10 x 0.46 cm 10 x 0.4 cm
ODS 1 3 TR-013200 TR-413200 TR-025420 TR-417050 TR-013201 TR-413201

Si 3 TR-013210 TR-413210 TR-025424 TR-417054 TR-013211 TR-413211
C1 3 TR-013215 TR-413215 TR-025426 TR-417056 TR-013216 TR-413216
C6 3 TR-013220 TR-413220 TR-025428 TR-417058 TR-013221 TR-413221
C8 3 TR-013226 TR-413226 TR-025430 TR-417060 TR-013227 TR-413227
CN 3 TR-013231 TR-413231 TR-025432 TR-417062 TR-013232 TR-413232
NH2 3 TR-013236 TR-413236 TR-025434 TR-417064 TR-013237 TR-413237
Phenyl 3 TR-013241 TR-413241 TR-025436 TR-417066 TR-013242 TR-413242
Particle size (µm) 15 x 0.46 cm 15 x 0.4 cm 20 x 0.46 cm 20 x 0.4 cm 25 x 0.46 cm 25 x 0.4 cm

Si 3 TR-013212 TR-413212 TR-013213 TR-413213 TR-013214 TR-413214
C1 3 TR-013217 TR-413217 TR-013218 TR-413218 TR-013219 TR-413219
C6 3 TR-013222 TR-413222 TR-013223 TR-413223 TR-013224 TR-413224
C8 3 TR-013228 TR-413228 TR-013229 TR-413229 TR-013230 TR-413230
CN 3 TR-013233 TR-413233 TR-013234 TR-413234 TR-013235 TR-413235
NH2 3 TR-013238 TR-413238 TR-013239 TR-413239 TR-013240 TR-413240
Phenyl 3 TR-013243 TR-413243 TR-013244 TR-413244 TR-013245 TR-413245
232
Tracer Extrasil
Microbore columns Tracer EXTRASIL Semi-Preparative columns Tracer EXTRASIL
Function D i m e n s i o n s Function D i m e n s i o n s
Particle size (µm) 10 x 0.21 cm 20 x 0.21 cm 10 x 0.3 cm 20 x 0.3 cm Particle size (µm) 15 x 0.7 cm 25 x 0.7 cm 15 x 1.0 cm 25 x 1.0 cm
ODS1 5 TR-021200 TR-021201 TR-021236 TR-021237 ODS1 5 TR-014501 TR-014502 TR-014503 TR-014504
ODS2 5 TR-021202 TR-021203 TR-021238 TR-021239 ODS2 5 TR-014505 TR-014506 TR-014507 TR-014508
Si 5 TR-021204 TR-021205 TR-021240 TR-021241 Si 5 TR-014509 TR-014510 TR-014511 TR-014512
C1 5 TR-021206 TR-021212 TR-021242 TR-021243 C1 5 TR-014513 TR-014514 TR-014515 TR-014516
CN 5 TR-021211 TR-021213 TR-021248 TR-021249 CN 5 TR-014525 TR-014526 TR-014527 TR-014528
NH2 5 TR-021214 TR-021215 TR-021250 TR-021251 NH2 5 TR-014529 TR-014530 TR-014531 TR-014532
Phenyl 5 TR-021216 TR-021217 TR-021252 TR-021253 Phenyl 5 TR-014533 TR-014534 TR-014535 TR-014536
SAX 5 TR-021218 TR-021219 TR-021254 TR-021255 SAX 5 TR-014537 TR-014538 TR-014539 TR-014540
SCX 5 TR-021220 TR-021221 TR-021256 TR-021257 SCX 5 TR-014541 TR-014542 TR-014543 TR-014544
ODS1 10 TR-014545 TR-014546 TR-014547 TR-014548
ODS2 10 TR-014549 TR-014550 TR-014551 TR-014552
TEKNOKROMA CAN SUPPLY OTHER COMBINATIONS Si 10 TR-014553 TR-014554 TR-014555 TR-014556

C6 10 TR-014557 TR-014558 TR-014559 TR-014560
OF DIAMETER AND LENGTH ON APPLICATION
CN 10 TR-014565 TR-014566 TR-014567 TR-014568
NH2 10 TR-014569 TR-014570 TR-014571 TR-014572
For Guard Columns please refer to pages 193-196 Phenyl 10 TR-014573 TR-014574 TR-014575 TR-014576
SAX 10 TR-014577 TR-014578 TR-014579 TR-014580
SCX 10 TR-014581 TR-014582 TR-014583 TR-014584
233
Hyperpack ODS
Due to its characteristics of pore size, surface area, percentage 5 Microns Packing
of covering (%C), and the kind of silica it is build of, it is the Microbore columns 0.21 cm i.d. HYPERPACK
suitable alternative to Hypersil ODS packings. Its chromatographic
behavior exactly reproduces the one of this popular packing, being Length Diameter
able to transfer the chromatographic methods without any kind of Packing Funct. µm cm cm Cat.Nbr.
adjustment. Hyperpack ODS 5 3 0.21 TR-010272
Hyperpack ODS 5 5 0.21 TR-010273
5 Microns Packing Hyperpack ODS 5 10 0.21 TR-010274
Analytical columns 0.46 cm i.d. HYPERPACK Hyperpack ODS 5 15 0.21 TR-010275
Length Diameter Hyperpack ODS 5 20 0.21 TR-010276
Packing Funct. µm cm cm Cat.Nbr. Hyperpack ODS 5 25 0.21 TR-010277

Hyperpack C8 5 3 0.21 TR-011028

Hyperpack C8 5 5 0.21 TR-011029

Hyperpack C8 5 10 0.21 TR-011030

Hyperpack C8 5 15 0.21 TR-011031

Hyperpack C8 5 20 0.21 TR-011032

Hyperpack C8 5 25 0.21 TR-011033

Hyperpack C8 5 3 0.46 TR-011021
5 Microns Packing
Hyperpack C8 5 4 0.46 TR-011022 Microbore columns 0.3 cm i.d. HYPERPACK
Hyperpack C8 5 5 0.46 TR-011023 Length Diameter
Hyperpack C8 5 10 0.46 TR-011024 Packing Funct. µm cm cm Cat.Nbr.
Hyperpack C8 5 15 0.46 TR-011025 Hyperpack ODS 5 3 0.3 TR-010278
5 Microns Packing Hyperpack ODS 5 20 0.3 TR-010282
Analytical columns 0.4 cm i.d. HYPERPACK Hyperpack ODS 5 25 0.3 TR-010283
Length Diameter Hyperpack C8 5 3 0.3 TR-011160
Packing Funct. µm cm cm Cat.Nbr. Hyperpack C8 5 5 0.3 TR-011034
Hyperpack ODS 5 3 0.4 TR-411000 Hyperpack C8 5 10 0.3 TR-011035

Hyperpack ODS 5 20 0.4 TR-411005 5 Microns Packing
Hyperpack ODS 5 25 0.4 TR-411006 Semi-preparative columns HYPERPACK
Hyperpack C8 5 3 0.4 TR-410081 Length Diameter
Hyperpack C8 5 4 0.4 TR-410082 Packing Funct. µm cm cm Cat.Nbr.
234
Hyperpack ODS
Length Diameter Hyperpack C8 3 20 0.4 TR-011064
Packing Funct. µm cm cm Cat.Nbr. Hyperpack C8 3 25 0.4 TR-011065
Hyperpack ODS 5 5 2.12 TR-010290 3 Microns Packing
Hyperpack ODS 5 10 2.12 TR-010291 Microbore columns 0.21 cm i.d. HYPERPACK
Length Diameter
Hyperpack C8 5 10 0.78 TR-011039
Hyperpack C8 5 15 0.78 TR-011040
Hyperpack C8 5 25 0.78 TR-011041
Hyperpack C8 5 10 1.00 TR-011042
Hyperpack C8 5 15 1.00 TR-011043
Hyperpack C8 5 25 1.00 TR-011044
Hyperpack C8 5 5 2.12 TR-011045
Hyperpack C8 3 3 0.21 TR-011066
Hyperpack C8 5 10 2.12 TR-011046
Hyperpack C8 3 5 0.21 TR-011067
Hyperpack C8 5 15 2.12 TR-011047
Hyperpack C8 3 10 0.21 TR-011068
Hyperpack C8 5 25 2.12 TR-011048
Hyperpack C8 3 15 0.21 TR-011069
Hyperpack C8 3 20 0.21 TR-011070
3 Microns Packing Hyperpack C8 3 25 0.21 TR-011071
Ultrarapid columns 0.46 cm i.d. HYPERPACK
Length Diameter 3 Microns Packing
Packing Funct. µm cm cm Cat.Nbr. Microbore columns 0.3 cm i.d. HYPERPACK
Length Diameter
Hyperpack C8 3 3 0.46 TR-011053
Hyperpack C8 3 4 0.46 TR-011054
Hyperpack C8 3 5 0.46 TR-011055
Hyperpack C8 3 10 0.46 TR-011056
Hyperpack C8 3 15 0.46 TR-011057
Hyperpack C8 3 20 0.46 TR-011058
Hyperpack C8 3 25 0.46 TR-011059
3 Microns Packing
Analytical columns 0.4 cm i.d. HYPERPACK
Length Diameter
Hyperpack C8 3 4 0.4 TR-011060
Hyperpack C8 3 5 0.4 TR-011061
Hyperpack C8 3 10 0.4 TR-011062
Hyperpack C8 3 15 0.4 TR-011063
235
Hyperpack Basic
Length D
iameter
Hyperpack BASIC C8 5 3 0.4 TR-411108
5 Microns Packing
Microbore columns 0.21 cm i.d. HYPERPACK BASIC
Length D
iameter
Hyperpack BASIC ODS 5 3 0.21 TR-010317
It reproduces with total fidelity the chromatographic behavior of Hyperpack BASIC ODS 5 5 0.21 TR-010318
the columns Hypersil BDS C18.
Available in 3 and 5µm and in all length and diameter
configurations.
5 Microns Packing Hyperpack BASIC C8 5 3 0.21 TR-011115
Analytical columns 0.46 cm i.d. HYPERPACK BASIC Hyperpack BASIC C8 5 5 0.21 TR-011116
Length D
iameter
Hyperpack BASIC ODS 5 4 0.46 TR-011008 Hyperpack BASIC C8 5 25 0.21 TR-011120

5 Microns Packing
Hyperpack BASIC ODS 5 15 0.46 TR-011011 Microbore columns 0.3 cm i.d. HYPERPACK BASIC
Hyperpack BASIC ODS 5 20 0.46 TR-011012 Length Diameter
Hyperpack BASIC ODS 5 25 0.46 TR-011013 Packing Funct. µm cm cm Cat.Nbr.
Hyperpack BASIC C8 5 3 0.46 TR-011108 Hyperpack BASIC ODS 5 3 0.3 TR-010323
Hyperpack BASIC C8 5 25 0.46 TR-011114 Hyperpack BASIC C8 5 3 0.3 TR-011121
5 Microns Packing Hyperpack BASIC C8 5 10 0.3 TR-011123
Analytical columns 0.4 cm i.d. HYPERPACK BASIC Hyperpack BASIC C8 5 15 0.3 TR-011124
Length Diameter Hyperpack BASIC C8 5 20 0.3 TR-011125
Packing Funct. µm cm cm Cat.Nbr. Hyperpack BASIC C8 5 25 0.3 TR-011126
5 Microns Packing
Hyperpack BASIC ODS 5 5 0.4 TR-411009 Semi Preparative columns HYPERPACK BASIC
Hyperpack BASIC ODS 5 10 0.4 TR-411010 Length Diameter
Hyperpack BASIC ODS 5 20 0.4 TR-411012 Hyperpack BASIC ODS 5 10 0.78 TR-010329
236
Hyperpack Basic
Length Diameter 3 Microns Packing
Packing Funct. µm cm cm Cat.Nbr. Ultrarapid Columns 0.4 cm i.d. HYPERPACK BASIC
Length Diameter
5 Microns Packing 3 Microns Packing

Cartridge System 0.4 cm i.d. HYPERPACK BASIC Microbore Columns 0.21 cm i.d.HYPERPACK BASIC
Length Diameter
Length Diameter
Hyperpack BASIC ODS 5 7,5 0.4 TR-010339
Hyperpack BASIC C8 5 7,5 0.4 TR-011137
3 Microns Packing
Analytical Columns 0.46 cm i.d. HYPERPACK BASIC Hyperpack BASIC C8 3 20 0.21 TR-011152
Length Diameter Hyperpack BASIC C8 3 25 0.21 TR-011153

Hyperpack BASIC ODS 3 3 0.46 TR-011014 3 Microns Packing
Hyperpack BASIC ODS 3 4 0.46 TR-011015 Microbore Columns 0.3 cm i.d. HYPERPACK BASIC
Hyperpack BASIC ODS 3 5 0.46 TR-011016 Length D
iameter
237
Lichrosorb
This traditional irregular packing is packed following completely With this irregular packing, the efficiencies normally obtained are
opitimized methods, ensuring maximum efficiency, stability and of 30-40000 N/m for the 5 µm packings, and 50-70,000 N/m for
reproductibility in all the columns. the 5 µm.
Analytical columns LICHROSORB

RP-8 5 TR-011441 TR-411441 TR-011443 TR-411443 TR-011445 TR-411445 TR-011447 TR-411447 TR-011449 TR-411449
RP-Select B 5 TR-016046 TR-416046 TR-016047 TR-416047 TR-011969 TR-411969 TR-016048 TR-416048 TR-011970 TR-411970
Diol 10 TR-011511 TR-411511 TR-011513 TR-411513 TR-011515 TR-411515 TR-011517 TR-411517 TR-011519 TR-411519
Semi-preparative Tracer columns LICHROSORB

Function D i m e n s i o n s
Particle size (µm) 15 x 0.7 cm 25 x 0.7 cm 15 x 1.0 cm 25 x 1.0 cm
RP-18 7 TR-014429 TR-014431 TR-014433 TR-014436
For Guard Columns please refer to pages 193-196
238
Lichrospher
Lichrospher´s spherical packing of 5 and 10 µm particle size, giving

all the advantages that are common to all the spherical packings:
high permeability, high efficiency and excellent column stability.
Analytical columns LICHROSPHER

Si 100 5 TR-011541 TR-411541 TR-011543 TR-411543 TR-011545 TR-411545 TR-011547 TR-411547 TR-011549 TR-411549
100 RP-18 5 TR-011551 TR-411551 TR-011553 TR-411553 TR-011555 TR-411555 TR-011557 TR-411557 TR-011559 TR-411559
100 RP-18 ec 5 TR-011561 TR-411561 TR-011563 TR-411563 TR-011565 TR-411565 TR-011567 TR-411567 TR-011569 TR-411569
100 NH2 5 TR-011591 TR-411591 TR-011593 TR-411593 TR-011595 TR-411595 TR-011597 TR-411597 TR-011599 TR-411599
100 CN 5 TR-011601 TR-411601 TR-011603 TR-411603 TR-011605 TR-411605 TR-011607 TR-411607 TR-011609 TR-411609
100 Diol 5 TR-011611 TR-411611 TR-011613 TR-411613 TR-011615 TR-411615 TR-011617 TR-411617 TR-011619 TR-411619
60 RP-Select B 5 TR-016813 TR-416813 TR-016814 TR-416814 TR-016815 TR-416815 TR-016816 TR-416816 TR-016817 TR-416817
100 CN 10 TR-011681 TR-411681 TR-011683 TR-411683 TR-011685 TR-411685 TR-011687 TR-411687 TR-011689 TR-411689
60 RP-Select B 10 TR-016808 TR-416808 TR-016809 TR-416809 TR-016810 TR-416810 TR-016811 TR-416811 TR-016812 TR-416812
Semi-preparative Tracer columns LICHROSPHER

RP-18 10 TR-014437 TR-014439 TR-014441 TR-014443
RP-18 EC 10 TR-014445 TR-014447 TR-014449 TR-014451
Microbore Tracer columns LICHROSPHER

RP-18 5 TR-021069 TR-021071 TR-021293 TR-021294
RP-18 EC 5 TR-021073 TR-021075 TR-021295 TR-021296
A totally porous spherical packing, with a particle size of 4µm,

giving a compromise alternative between the packings of 3 and
5µm.
Analytical columns SUPERSPHER

For Guard Columns please refer to pages
239
Tracer Carbohydrates
Tracer Carbohydrates
Tracer Carbohydrates column is based on a Polymeric Coating
Chemically Bonded Silica Base packing, that includes in its structure
primary amino groups.
This polymeric coating protects surface silica packing against attacks

Sugars Separation
coming from aqueous/organic mobile phases used in the separation
of sugars. Column: TR-016805, Tracer Carbohydrates,
4.6 x 250 mm
Keeping the selectivity of a conventional NH2 column, Tracer Eluent: 75/25 acetonitrile/water
Carbohydrates have a longer lifetime. Flow: 0.9 ml/min
This column can be also washed with eluents lightly basic to eliminate Temperature: 35ºC
acidic impurities irreversibly linked with the packing that degrade very Detector: IR
Protein Standards
quickly any standard NH2 columns. 1. Fructose
2. Gructose
3. Sucrose
• Higher Stability 4. Maltose
• Better resolution in disaccharides analysis 5. Lactose
Carbohydrates Columns
Designed for Carbohydrates Analisys
Reference Description Particle Size Dimensions

µm cm
TR-016804 Tracer Carbohydrates 5 μm 15 x 0.46 cm
240
Tracer Excel PFP & PAH
Tracer Excel PFP Tracer Excel PAH

Excel PFP is a endcapped stationary phase with unique Ultra High pure silica particle, Excel PAH is a polymeric C18
selectivity that can be useful for separating halogen-containing bonded phase that creates a three-dimensional network
compounds, polar analytes, and compounds that contain minor stationary phase with an optimal selectivity for PAHs
structural differences. Excel PFP can be used at high temperatures separations.
(50ºC) over a pH range of 2-8.
Excel PAH provides a base-line resolution of 16 PAHs priority
• Ideal for HPLC and UHPLC pollutants in EPA Method 610.
• Different selectivity from C18 or C8 with polar interaction and
π-π interaction that plays a major role in the separation • USP: L1 polymeric C18 Bonding
• Superior chemical durability • Particle size: 5 µm
• Identical selectivity over different particle sizes • Pore size: 300 Å
• USP L43 • Surface Area: 100 m2/g
• Carbon Load: 28%
• Particle: 5 μm • PH Range: 2-8
• Pore Size: 120 Å
• Carbon Load: 11%
• End-Cap: yes Column: TR-021615 Tracer Excel PAH, 5u 15 x 0.46 cm Eluent: ACN (A) H2O
• Surface Area: 300 m2/g (B); Flow rate: 1,5 ml/min; Temperature: 35ºC; Detector: UV, 254 nm
• pH Range: 2.5 to 8
t ACN H2O 1. Naphtalene 9. Benz[a]anthracene
• Maximum Temperature: 80 °C 2. Acenaphtylene 10. Chrysene
0 50 50
3. Acenaptheno 11. Benzo[b]fluoranthene
5 50 50 4. Fluorene 12. Benzo[k]fluoranthene
Excel PFP have a retention and selectivity of charged bases, 15 100 100 5. Phenatrene 13. Benzo[a]pyrene
electronegative compounds, and amine-containing compounds. 20 100 100 6. Anthracene 14. Dibenz[a,h]anthracene
Unlike a conventional cyano column. This versatile column is also 7. Fluoranthene 15. Benzo[g,h,i]perylene
compatible with highly aqueous mobile phases. 8. Pyrene 16. Indeno[1,2,3-c,d]pyrene
Column: TR-021520 Tracer Excel PFP, 1. o-Cresol

5 µ 4.6 x 250 mm 2. m-Cresol
Eluent: acetonitrile, 0.1% formic 3. 3,4-Dimethylphenol
acid 35 water, 4. 3,5-Dimethylphenol
0.1% formic acid 65 5. 2,5-Dimethylphenol
Flow: 1 ml/min 6. 2,6-Dichlorophenol
Temperature: 35ºC 7. 2,3-Dichlorophenol
Detector: UV 280 nm 8. 2,4-Dichlorophenol
9. 3,4-Dichlorophenol
10. 2,4-Dibromophenol
11. 3,5-Dibromophenol
Length Diameter
Tracer Excel PAH PAH 5 15 0.46 TR-021615
Tracer Excel PAH PAH 5 25 0.46 TR-021620
Length D iameter
Tracer Excel PFP PFP 5 15 0.46 TR-021515
Tracer Excel PFP PFP 5 25 0.46 TR-021520
241
Fingertight Fittings
High Pressure Fingertight Fittings
One Piece or Two? Two-Piece Fingertight Fittings

A one-piece fitting is more convenient and less cumbersome, Our original Two-Piece Fingertight Fittings were designed
since the ferrule cannot stick in a receiving port and the fitting exclusively for 1/16" OD tubing. We now offer optional ferrules for
is more easily found if dropped. With two-Piece Fingertight, you connecting 1/32"OD and 190 µm OD tubing with any Fingertight
only replace the ferrule instead of the entire unit, making these nut on last page. Our M-215 Conductive Perfluorelastomer Ferrule
Fingertights more economical than the one-piece version. is designed for mass spectrometer electrospray applications.
One Piece Fingertight Fittings

All of our One-Piece Fingertight Fittings are designed to be used
with 1/16´´ OD tubing, except the M-645 (1/32´´) and P-100 (1/8´´).
UP-F-200 UP-F-300
10-32 DelrinTM Winged Nut 10-32 PEEKTM Double Winged Nut
with F-142 PEEKTM Ferrule with F-142 PEEKTM Ferrule
UP-F-100 UP-F-120
10-32 Kel-FTM Fiting 10-32 PEEKTM Fiting
Max Pressure 276 bar Max Pressure 414 bar
UP-F-330 UP-F-331
10-32 PEEKTM Nut 10-32 PEEKTM Nut
UP-F-127 UP-F-130
10-32 PEEKTM Fiting 10-32 PEEKTM Fiting
Max Pressure 414 bar Max Pressure 414 bar
UP-F-140 UP-F-150
10-32 Stainless Steel Nut 10-32 Stainless Steel Nut
To order please follow these guidelines:

UP-M-645 UP-P-100
6-40 PEEK/Kel-FTM Fiting 1/14-28 Kel-FTM Fiting 1/16" OD tubing
for 1/32´´ OD tubing for 1/8´´ OD tubing Select the desired nut, which comes complete with the appropriate
Max Pressure 121-224 bar Max Pressure 69 bar
ferrule. Or order the optional UP-F-142N Ferrule, along with the
desired nut by replacing the "x" at the end of its product number
with "-01". For instance, if you want an UP-F-113 Ferrule Nut, order
One-Piece Fingertight Fittings1,2 UP-F-200-01, not UP-F-200x.
Cat.No. Description Qty. Please note: "-0.1" denotes a single nut without the ferrule.
UP-F-100x Kel-F, Red, 10-32 for 1/16” OD tubing 10-pk
UP-F-120x PEEK, Natural, 10-32 1/16” OD tubing 10-pk 1/32" OD tubing
UP-F-127x PEEK, Natural, 10-32 Short 1/16” OD tubing 10-pk Select the UP-F-113 Ferrule. Order the desired nut by replacing the
UP-F-130x PEEK, Natural, 10-32 Long 1/16” OD tubing 10-pk "x" at the end of its product number with "-0.1", as described above.
UP-M-645x PEEK/Kel-F, Natural, 6-40, for 1/32” OD tubing 10-pk
190 µm OD tubing
UP-P-100 Kel-F, Natural, 1/4-28, for 1/8” OD tubing ea.
Select the UP-F-148 Ferrule. Order the desired nut by replacing
the "x" at the end of its product number with "-0.1", as described
above.
242
Fingertight Fittings - Stainless Steel Tubing
360-510 µm OD tubing Stainless Steel Tubing
For electrospray applications, chose the UP-M-215 Conductive
Ferrule. Order the desired nut by replacing the "x" at the end of its • Precut 316 Stainless Steel*
product number with "-0.1", as described at previous page. • The Cleanest, Best Finish available
• Color-Coded Banding for easy identification
Two-Piece Polymer Fingertight Fittings
Cat.No. Description Qty.
UP-F-200x Delrin Nuts, Red, with UP-F-142 Ferrules, 10-32 10-pk
UP-F-300x PEEK Nuts, Natural, with UP-F-142 Ferrules, 10-32 10-pk
UP-F-330x Long PEEK Nuts, Natural, with UP-F-142 Ferrules, 10-32 10-pk
UP-F-331x Short PEEK Nuts,Natural, with UP-F-142 Ferrules,10-32 10-pk

Two-Piece Stainless Steel Fingertight Fittings
UP-F-140x Stainless Steel Nuts, with UP-F-142 Ferrules, 10-32 10-pk
UP-F-150x Long Stainless Steel Nuts, with UP-F-142 Ferrules, 10-32 10-pk
UP-F-113
Optional PEEK
UP-F-142N
Optional TefzelTM
UP-F-148
Optional Kel-FTM
UP-M-215
Optional
Stainless Steel, .005" Stainless Steel, .010"
Ferrule Ferrule Ferrule Perfluoroelastometer (125µm) ID x 1/16" OD (.25mm) ID x 1/16" OD
for 1/32" OD tubing for 1/16" OD tubing for 190µm OD tubing Ferrule
for 360-510µm OD (Red Colour Band) (Blue Colour Band)
tubing (conductive)
Replacement Ferrules Reference Length Reference Length
Cat.No. Description Qty. UP-U-152 5 cm UP-U-111 5 cm
For 1/16” OD Tubing UP-U-153 10 cm UP-U-112 10 cm
UP-F-142x PEEK Ferrules, Natural 10-pk UP-U-154 20 cm UP-U-113 20 cm
UP-F-142Nx Tefzel Ferrules, Natural 10-pk UP-U-155 30 cm UP-U-114 30 cm
For 1/32” OD Tubing UP-U-156 0.5 m UP-U-132 0.5 m
UP-F-113 PEEK Ferrules, Natural ea. UP-U-157 1 m** UP-U-133 1 m**
For 360-510 µm OD Tubing UP-U-158 1.5 m** UP-U-106 1.5 m**
UP-F-151 Kel-F Ferrule, Natural ea. UP-U-160 7.5 m** UP-U-162 7.5 m**
UP-M-215 Conductive Perfluoroelastomer Ferrules, Black ea.
For 190 µm OD Tubing
UP-F-148 Kel-F Ferrule, Natural ea. Stainless Steel, .007" Stainless Steel, .020"
(175µm) ID x 1/16" OD (.50mm) ID x 1/16" OD
(Black Colour Band) (Yellow Colour Band)
Reference Length Reference Length
UP-U-126 5 cm UP-U-101 5 cm
UP-U-127 10 cm UP-U-102 10 cm
UP-U-128 20 cm UP-U-103 20 cm
UP-U-129 30 cm UP-U-104 30 cm
UP-U-130 0.5 m UP-U-134 0.5 m
UP-U-131 1 m** UP-U-135 1 m**
UP-U-108 1.5 m** UP-U-105 1.5 m**
UP-U-161 7.5 m** UP-U-163 7.5 m**
** All Stainless Steel tubing of longer than 1m is coiled.
243
PEEK™ tubing
PEEK™ Tubing PEEK tubing 1/16" OD X 5'
Reference Description Colour psi bar
• 1/16", 1/8" and 1.8mm ODs Available UP-1560 .0025" (65 µm)ID Natural 7,000 psi (483 bar)*
• Biocompatible, Inert and Easily Cut UP-1561 .004" (100 µm)ID Black 7,000 psi (483 bar)*
• Great for High Pressure Applications UP-1535 .005" (125 µm) ID Red 7,000 psi (483 bar)*
UP-1562 .006" (150 µm)ID Purple 7,000 psi (483 bar)*
UP-1536 .007" (175 µm) ID Yellow 7,000 psi (483 bar)*
UP-1531 .010" (.25 mm) ID Natural 7,000 psi (483 bar)*
UP-1531B .010" (.25 mm) ID Blue 7,000 psi (483 bar)*
UP-1565 .015" (.40 mm) ID Gray 7,000 psi (483 bar)*
UP-1532 .020" (.50 mm) ID Orange 7,000 psi (483 bar)*
UP-1533 .030" (.75 mm) ID Green 7,000 psi (483 bar)*
UP-1538 .040" (1.00 mm) ID Natural 5,000 psi (345 bar)*
UP-1537 .055" (1.40 mm) ID Natural 500 psi (345 bar)*

PEEK tubing 1/8" OD X 5'
PEEK tubing 1.8 mm OD X 5'

PEEK (polyetheretherketone) polymer tubing is biocompatible, UP-1545 .010" (.25 mm) ID Natural 7,000 psi (483 bar)*
chemically inert to most solvents, and can be used to replace UP-1546 .020" (.50 mm) ID Natural 7,000 psi (483 bar)*
stainless steel tubing in most liquid analytical systems. Unlike UP-1547 .030" (.75 mm) ID Natural 7,000 psi (483 bar)*
stainless steel and titanium tubing, PEEK tubing is flexible and can UP-1540 .042" (1.05 mm) ID Natural 5,000 psi (345 bar)*
be easily cut to desired lengths. PEEK tubing can be used with UP-1539 .055" (1.40 mm) ID Natural 500 psi (34 bar)*
stainless steel or polymer fittings.
The benefits of PEEK polymer tubing include a high pressure rating Polymer Tubing Cutters
(up to 7,000 psi in most cases) and a high temperature rating for 1/16", 1/8", 3/16", 1/4" and 5/16 OD tubing
(maximum continuous use temperature of 100ºC). Additionally,
PEEK tubing has a very smooth internal surface, which causes
less turbulance than similar sized metal tubing. Turbulence can
cause remixing of separated sample bands and dilution of bands
by the mobile phase. Of all our polymer tubing materials, PEEK is
the least permeable to gas.
Polymer tubing cutters

UP-A-327 Standard Polymer Tubing Cutter* for 1/16" and 1/8"
OD tubing
UP-A-329 Large Bore Polymer Tubing Cutter* for 3/16" - 5/16"
OD tubing
UP-A-328 Replacement Blades for A-327 and A-329 - 5 pk.
244
Inlet Solvent Filters
General Use Inlet Solvent Filters General Use Inlet Filters
For Analytical HPLC
• Large Surface Areas Reference Description
• Disposable UP-A-220 10µm Inlet Solvent Filter, for 1/8" OD tubing1
• 2 µm, 10 µm and 20 µm Filters Available
UP-A-221 UP-A-220, 5-pack, for 1/8" OD tubing1
• General use and Prep Filters for Higher Flow Applications
UP-A-222 2µm Inlet Solvent Filter, for 1/8" OD tubing1
It is good practice to filter your solvents to prevent pump damage. UP-A-223 UP-A-222, 5-pack, for 1/8" OD tubing1
These 316 stainless steel filters provide that protection. Their large UP-A-228 2µm Inlet Solvent Filter with stem, for 1/8" ID tubing
surface areas also mean longer life without pump cavitation. UP-A-302 10µm Inlet Solvent Filter with stem, for 1/16" ID tubing
UP-A-302A 10µm Inlet Solvent Filter with Flangeless Fittings, for 1/8"
Because filters should be changed periodically, we make it easy OD tubing2
to replace them, without tools. For those filters using a plastic nut, UP-A-309 10µm Inlet Solvent Filter with stem, for 1/16" ID tubing
thread the nut into the filter and finger tighten. Our other filters have
stems, allowing easy insertion directly into your inlet tubing. For Waters'" Analytical HPLC Systems
Please Note: The internal design of the UP-A-309 and the UP-A-
UP-A-231A 20µm Inlet Solvent Filter for 3/16” OD tubing3
230A Filters allows solvent to be drawn to within 1/8" (3.2mm) of the
bottom of your solvent bottle, with Bottom-of-the-BottleTM. designs UP-A-310 10µm Inlet Solvent Filter with stem, for 1/8” tubing
similar to the stainless steel and UHMWPE filters.
For Preparative HPLC Systems
UP-A-225 20µm Inlet Solvent Filter with stem, for 1/16" ID tubing
UP-A-225A 20µm Inlet Solvent Filter with Flangeless Fittings, for 1/8
OD tubing4
UP-A-226A 10µm Inlet Solvent Filter, for 5/16" OD tubing5
UP-A-222 UP-A-230A 20µm Inlet Solvent Filter, for 1/4" OD tubing6
2µ
shown with UP-P-100
Fingertight UP-A-311 10µm Inlet Solvent Filter with stem, for 1/16" ID tubing
Nut (not included) UP-A-302
For 1/8 OD tubing 10 µm UP-A-311A 10µm Inlet Solvent Filter with Flangeless Fittings,
For 1/16 OD tubing for 1/8" OD tubing2
1
Requires a UP-P-100 Fitting (not included). Order the UP-A-210 Kit above,
or purchase the UP-P100 alone.
2
Includes a UP-P-315 Tefzel™ (ETFE) Nut and a UP-P-300 ETFE Ferrule.
3
Includes a UP-P-132 PEEK™ Nut and a UP-P-133 ETFE Ferrule.
4
Includes a UP-P-315 ETFE Nut and a UP-P-300N ETFE Ferrule.
5
Includes a UP-U-622 PEEK Nut and a UP-U-660 ETFE Ferrule.
6
Includes a UP-U-655 PEEK Nut and a UP-U-650 ETFE Ferrule.
UP-A-302A
10 µm
With UP-P-300
Flangeless Fittings
UP-A-310
10 µm
Maximum Suggested Flow Rates
For 1/8 OD tubing For 1/8 ID tubing Flow Rates are determined by porosity surface area
Reference Porosity Max. Flow Rate
UP-A-222 2 µm 10 mL/min.
UP-A-302A 10 µm 40 mL/min.
UP-A-225 20 µm 100 mL/min.
UP-A-225A UP-A-311 10 µm 100 mL/min.
20 µm UP-A-230A
With UP-P-315-UP-P-300N 20 µm UP-A-311A 10 µm 100 mL/min.
Flangeless Fittings With UP-U-655/-UP-U-650
For 1/8 OD tubing Flangeless Fittings
For 1/4 OD tubing
245
Bottle Caps - PEEK Bottom-of-the-Bottle Solvent Filters
PEEK Bottom-of-the-Bottle Solvent Filters Bottle Caps
• Our Most Recommended Filtering Unit! • Inexpensive
• 100% PEEK Polymer Construction • Extremely Simple - No Threaded Ports or Fittings!
• Easy Operation - No fittings required!
If you are looking for a bottle cap that is quick and easy, but still
These Upchurch Scientific biocompatible filters are made from allows many options, we have just what you need!
100% PEEK polymer. Each has two PEEK frits. The bottom frit These injection-molded caps are manufactured of inert TefzelTM
(2µm or 10µm) will draw solvents from within 0.28” (2mm) of the and polypropylene. They fit standard GL-45 or smaller-neck GL-38
bottom of the solvent bottle. The 2µm frit on the side may be used bottles.
for a 1/8” OD helium sparging line. Three holes are provided in each insert. With two of the holes you
simply push your tubing straight through. The third hole, with a luer
To use, simply press fit your appropriately sized Teflon® tubing taper, can be used for a number of 0ptions. Any male luer will fit
firmly into the top holes. That´s It!. snugly in this hole, or you can use our UP-A-626 or UP-A-627 Plug.
The exceptions are the UP-A-610 and UP-A-610B Bottle Caps.
Please see the note to the right.
Port for solvent intake line
UP-A-620 UP-A-622
GL-45 Bottle Cap GL-38 Bottle Cap
Port for your sparging line For 1/8" tubing For 1/8" tubing
(optional use)
Filters and dispenses incoming

Draws solvent from sparging gas (optional use)
the bottom of your Holes for 1/8" 00 Holes for 1/8" 00
solvent bottle tubing tubing
Additional hole Additional hole

for luer for luer
Bottle Caps for UP-GL-45, 1L Bottles

UP-A-610 for 3/16" OD tubing, red
UP-A-610B for 3/16" OD tubing, blue
UP-A-435 UP-A-438 UP-A-620B for 1/8" OD tubing, blue
Bottom-of-the-Bottle Filter Bottom-of-the-Bottle Filter
(for small neck bottles)
UP-A-630B for 1/16" OD tubing, blue
UHMWPE Bottom-of-the-Bottle Solvent Filters
Bottle Caps for UP-GL-38, 4L Bottles
UP-A-445 10µm UHMWPE Filter Assembly for 1/16” OD tubing 1
UP-A-622 for 1/8" OD tubing, black or white1

UP-A-446 10µm UHMWPE Filter Assembly for 1/8” OD tubing 2
UP-A-632 for 1/16" OD tubing, black or white1

UP-A-427 10µm UHMWPE Replacement Solvent Filter Cups, 5pk
UP-A-626 UP-P-600
PEEK Bottom-of-the-Bottle Solvent Filters Bottle Cap Plug 20 µm Bottle Cap Adapter
stainless steelll frit to connect 1/32"
Reference Description OD tubing
UP-A-435 2µm PEEK Filter for 1/8” OD tubing UP-A-629

Filter Bottle Cap Plug
UP-A-436 2µm PEEK Filter for 3/16” OD tubing 3
UP-A-437 2µm PEEK Filter for 1/8” OD tubing for small-neck (GL-38) bottles Bottle Cap Plugs and Adapter
UP-A-438 10µm PEEK Filter for 1/8” OD tubing for small-neck (GL-38) bottles Reference Description
UP-A-440 10µm PEEK Filter for 1/8” OD tubing UP-A-626 Bottle Cap Plug for luer hole, UHMWPE
UP-A-441 10µm PEEK Filter for 3/16” OD tubing3 UP-A-627 Filter Bottle Cap Plug for luer hole, UHMWPE
UP-A-450 2µm PEEK Filter for 1/16” OD tubing with 20 µm stainless steel Irit
UP-A-451 10µm PEEK Filter for 1/16” OD tubing UP-A-628 Bottle Cap Plug for 1/16" or 1/8" hole, UHMWPE
UP-A-452 2µm PEEK Filter for 1/16” OD tubing for small-neck (GL-38) bottles UP-A-629 Filter Bottle Cap Plug for 1/16" or 1/8" hole,
UP-A-453 10µm PEEK Filter for 1/16” OD tubing for small-neck (GL-38) bottles UHMWPE with 20 µm stainless steel Irit
1
Includes a UP-P-200 Tefzel™ Ferrule and a UP-P-245 Teflon™ PFA Nut UP-P-600 Bottle Cap Adapter for 1/8" hole2, PEEK to
2
Includes a UP-P-300 Tefzel™ Ferrule and a UP-P-345 Teflon™ PFA Nut
3
Typically for Waters® systems. connect 1/32" OD tubing
Designed for use with The UP-A-622 and UP-A-632 Bottle Cap rings now come in black or
1
white, depending on availability
246
Precolumn and Inline Filters
Increase the Life of your Column Ultra-Low Volume Precolumn Filter
A precolumn filter placed between the sample injection valve and • Our Lowest Swept Volume Precolumn Filter for 1/16" OD
the HPLC column protects the column from particles originating in Tubing
the sample and from pump/valve seal wear. Why use a precolumn
filter when there is a frit at the head of the column itself? Because With a .010" diameter thru-hole, our UP-A-318 Filter has one of
changing the column frit risks ruining the column by disturbing the the lowest swept volumes (0.61\µL*) of any HPLC filter available,
column packing. A precolumn filter provides relatively inexpensive ensuring maximum protection with no band broadening. Pressure
insurance against column damage, and changing its frit is easy. rated to 9,000 psi (620 bar).
Precolumn Filters
• O.5 µm or 2 µm Frits Available
• Great Column Protection
• Stainless Steel and Biocompatible PEEKTM Polymer Versions
Available
UP-A-318
These Precolumn Filters have .020" diameter thru-holes and 8° Precolumn Filter UP-A-102
distribution cones for minimal band spreading and mixing. They are 0.5 µm stainless steel frit
available in stainless steel (UP-A-315/UP-A-316), pressure rated

to 9,000 psi (620 bar) and biocompatible PEEK polymer versions Ultra-Low Volume Precolumn Filters
(UP-A-355/UP-A-356), pressure rated to 5,000 psi Reference Description Swept Volume*
(345 bar). Choose either the 0.5 µm or 2 µm version to filter UP-A-318 0.5µm Solvent Filter Assembly,
particulates from your flow path.
with UP-A-102 Frit 0.84 µL
UP-A-102x 0.5µm Replacement Frits, Stainless Steel,
10-pk 0.61 µL
Inline Solvent Filter

• Excellent for General Purpose In line Use
Placed between the pump and sample injection valve, our Inline
Solvent Filter traps particles released through normal piston seal
UP-A-315
Solvent Filter Assembly wear. Without an inline filter, these particles can be flushed through
UP-A-355
UP-A-315 Solvent FilterUP-A-355
Assembly your system's tubing to the sample injection valve, resulting in valve
Precolumn Filter PEEK Precolumn Filter damage and further system contamination.
This filter is pressure rated to 8,500 psi (586 bar) and uses a 2µm
Precolumn Filters stainless steel frit with a PEEK ring. An 8° distribution cone spreads
Reference Description Swept Volume* the flow of the mobile phase over the entire surface of the frit, while
UP-A-315 2µm Solvent Filter Assembly, the .050" diameter thru-hole allows proper solvent flow.
UP-A-316 0.5µm Solvent Filter Assembly,
UP-A-355 2µm Solvent Filter Assembly, with
UP-A-700 ID PEEK Frit, Biocompatible 1.4 µL
UP-A-356 0.5µm Solvent Filter Assembly, with
UP-A-701 ID PEEK Frit, Biocompatible 1.3 µL UP-A-314
Inline Solvent Filter
UP-A-101x 2µm Replacement Frits, Stainless Steel, UP-A-100
10-pk 0.74 µL
Inline Solvent Filters 2 µm stainless steel frit
Reference Description Swept Volume*

UP-A-314 2.0µm Solvent Filter Assembly,
10-pk 0.61 µL
UP-A-700 2µm Replacement Frit, PEEK Polymer 0.74 µL with UP-A-l 00 Frit 4.0 µL
UP-A-701 0.5µm Replacement Frit, PEEK Polymer 0.61 µL
10-pk 1.4 µL
* Swept volumes include/reflect theoretical frit volume values.
247
Deuterium Lamps for a Detector
Agilent Deuterium Lamps
Cat.No Model Description Ref. Agilent
TR-G1103-60001 Agilent 1100, 1200 Tungsten Lamp Visible Light Bulb G1103-60001
TR-LD-AGI-101 Agilent 1050 C 1050 DAD 1090, 1040 D2 Lamp 79883-60002
TR-LD-AGI-104LL Agilent 1100 VWD Longlife D2 Lamp 2140-0585
TR-LD-AGI-105 Agilent 1100 DAD 8453 D2 Lamp 2140-0590
TR-LD-AGI-105LL Agilent 1100, 1200 G1315/G1365 A & B Series DAD Longlife D2 Lamp 5182-1530
TR-LD-AGI-106 Agilent 8452 D2 Lamp 08452-60104
TR-LD-AGI-107LL Agilent 1100, 1200 G1315/G1365 C & D Series DAD Longlife D2 Lamp 2140-0585
TR-LD-AGI-108LL Agilent 1290 G4212 A/B DAD (8-Pin) Longlife D2 Lamp 5190-0917
Waters Deuterium Lamps

Cat.No Model Description Ref. Waters
TR-LD-WAT-102LL Waters 486 D2 Lamp WAT080678
TR-LD-WAT-104LL Waters 996, 2996 PDA Longlife D2 Lamp WAT052586
TR-LD-WAT-105LL Waters 2487 DAD Alliance Longlife D2 Lamp WAS081142
TR-LD-WAT-108LL Waters Acquity 2998 HB Longlife D2 Lamp 201000281
TR-LX-WAT-150MO Waters 470 474 2475 Xe Lamp WAT052665
Shimadzu Deuterium Lamps

Cat.No Model Description Ref. Shimadzu
TR-LD-SHI-100 Shimadzu LC4A LC6A SPD2A SPD6A SPD6AV SP4 D2 lamp 062-65056-03
TR-LD-SHI-101LL Shimadzu SPD10A, 10A3, 10AV, 10AVP, 20A, 20AV Longlife D2 Lamp 228-34016-02
TR-LD-SHI-102SLL Shimadzu UV series Spectrophotometer,BioSpec-mini Longlife D2 Lamp 200-75503-01
TR-LD-SHI-103LL Shimadzu LC2010 Longlife D2 Lamp 228-37401
TR-LD-SHI-104LL Shimadzu SPD-M10A, M10AV D2 Lamp 670-14604-00
TR-LD-SHI-105 Shimadzu Prominence 2030, Nexera 2040 D2 Lamp 228-55626-01
TR-LX-SHI-150MO Shimadzu RF1501.5301 5000 Xe Lamp 200-81500
TR-LX-SHI-150S Shim. RF540 RF535 RF551 RF500 RF10A RF10AXL Xe Lamp 228-45147
TR-LX-SHI-75XE Shimadzu RF530 RF510 Xe Lamp N/A
TR-LX-SHI-150SK Shimadzu RF5000U (w/Ozone) Xe Lamp UXL155
TR-LX-SHI-101 Shimadzu SPD-10AV,AVP,AVVP,20AV Tungsten Lamp 670-14602-00
Varian Deuterium Lamps
TR-LX-SHI-104 Shimadzu M10AVP/M20A Tungsten Lamp 228-34410-91
TR-LX-SHI-062-65005AT Shimadzu Spectrophotometer Tungsten Lamp 062-65005AT
Cat.No Model Description Ref. Varian

TR-LD-VAR-100LL Varian 2050 2550 5500 D2 Lamp 03-915503-99
TR-LD-VAR-103LL Varian UV50/100/200 9050 Prostar 310, Vista 5000, 5500 Longlife D2 Lamp 0391615691
TR-LD-VAR-104 Varian Prostar 330 DAD D2 Lamp 393570502
TR-LD-VAR-105LL Varian Prostar 325 335 Longlife D2 Lamp 110715400
TR-LD-VAR-106 Varian Prostar 340 345 D2 Lamp R000088515
TR-LX-VAR-152H Varian Prostar 363 Xe Lamp 392613103
248
Merck Hitachi Deuterium Lamps
Cat.No Model Description Ref. Hitachi
TR-LD-MEH-100S Hitachi L & U Series Nosed D2 Lamp HITA 890-2430
TR-LD-MEH-101 Hitachi 100-10, 124, 100-40, 100-50, 100-60 D2 Lamp 982-1035
TR-LX-MEH-152H Hitachi Fluorescence Detector Xe Lamp 5000330
TR-LD-MEH-103 Hitachi U-1800, 2810, 1900, 2900, 2910, 3900,3900H D2 Lamp 2J1-1500
TR-LT-MEH-100 Hitachi U Series Vis. Lamp 855-1200
TR-LT-MEH-101 Hitachi L2400, 2420, 2450, 2455 Tungsten Lamp 890-2527
Dionex Deuterium Lamps

Cat.No Model Description Ref. Dionex
TR-LD-DIO-101 Dionex DSA-1 VDM-1 D2 Lamp N/A
TR-LD-DIO-102LL Dionex PDA-100, PDA-3000, AD-25 Longlife D2 Lamp 939016T
TR-LD-DIO-104 Ultimate Dionex UVD 3000 Nano LC D2 Lamp 6074.2070
TR-LD-DIO-105LL Dionex Ultimate DAD,MWD 3000/RS VWD3000/3400 D2 Lamp 6074.1110
TR-LX-DIO-150S Dionex Fluoresence Detector Xe Lamp 5057-1000
TR-LT-DIO-105 Dionex 3000 VWD DAD Tungsten Lamp 6074.2000
Gilson Deuterium Lamps

Cat.No Model Description Ref. Gilson
TR-LD-GIL-101 Gilson115/116/117/118/119/151/152/155/156 D2 Lamp 100326
TR-LD-GIL-105LL Gilson 170 D.A.D. Longlife D2 Lamp 18011003
TR-LX-GIL-150MO Gilson 122 Xe Lamp N/A
TSP Deuterium Lamps

Cat.No Model Description Ref. TSP
TR-LD-TSP-102S TSP UV100/1000/2000/3000, Focus, CE Series D2 Lamp 9551-0023
TR-LD-TSP-106 TSP UV6000DAD,UV6000LP, Accela PDA, Surveyor D2 Lamp 108052
TR-LD-TSP-103 TSP SA6510,SP770,SP970,SP8480XR,SP8773XR D2 Lamp N/A
TR-LD-TSP-104 TSP Thermosolaar M Series D2 Lamp 942342020004
TR-LD-TSP-107 TSP ICE3300 AAS, S Series, SOLAAR S4 D2 Lamp 942342030004
TR-LT-TSP-106 TSP UV6000 Tungsten Lamp 60257-60006
ABI Deuterium Lamps
Cat.No Model Description Ref. ABI
TR-LD-ABI-100 ABI 757 759 783A 785A 1000S FS980 120A 130A D2 Lamp N2920149
249
Perkin Elmer Deuterium Cat.No Model Description Ref. Perkin Elmer
TR-LD-PER-100 Perkin Elmer Series 200 D2 Lamp N2920149
Lamps
TR-LD-PER-102 Perkin Elmer Series 200 DAD D2 Lamp N2922046
TR-LD-PER-103 PE LC235,1335,LC55,65,75,85,95,135 Lambda 1.3 Integral D2 Lamp N2351285
TR-LD-PER-160 PE Lambda 2 to 45 800 900 Bio, 55X series D2 Lamp B016-0917
TR-LT-PER-160 PE Lambda 2 to 45 800 900 Bio, 55X series W Lamp B011-4620
TR-LX-PER-150MO PE 203 204 650 MPF2A MPF3 MPF4 Xe Lamp 3501646
Jasco Deuterium Lamps Cat.No Model Description Ref. Jasco

TR-LD-JAS-101SLL Jasco UV970 975 (B & C)1570,1575,2075,3075, MD D2 Lamp 5330-0091
TR-LD-JAS-102LL Jasco V-530, 550, 560 570,600,700 & 7800 D2 Lamp 5330-0094B
TR-LT-JAS-102 Jasco V-530, 550, 560 570,600,700 Tungsten Lamp 5530-0099
TR-LX-JAS-150MO Jasco 820 821 Xe Lamp 5330-0052
TR-LX-JAS-274 Jasco 920 921 1520 2020 Xe Lamp 6715-H310H
Kontron Deuterium Lamps Cat.No Model Description Ref. Kontron

TR-LD-KON-102LL Kontron 535DAD, 332/335/430/432/433/770 Longlife D2 Lamp 91-91494
TR-LD-KON-103LL Kontron 540DAD 540+ 545V Longlife D2 Lamp 54-02007
TR-LD-KON-104 Kontron Uvikon 922, 923, 943, 930, 932, 933, 940 D2 Lamp 90-007825T
Kontron SFM25
TR-LX-KON-150MO Xe Lamp N/A
TR-LD-KON-108 Kontron 720LC D2 Lamp 54-02003
Biorad Deuterium Lamps Cat.No Model Description Ref. Biorad

TR-LD-BIO-101 Biorad 206, 300, 1790, BioDimensions, D2 Lamp 930-6106
TR-LD-BIO-103LL Biorad 1503 D2 Lamp N/A
Beckman Deuterium Cat.No Model Description Ref. Beckman

Lamps TR-LD-BEC-102 Beckman DU600 620 630 640 650 6000 7000 7500 D2 Lamp 514366
TR-LD-BEC-103 Beckman 166 D2 Lamp (prealigned) 239372
TR-LD-BEC-105 Beckman 168 D2 Lamp (prealigned) 538711
TR-LD-BEC-110 Beckman P/ACE MDQ D2 Lamp 144667
TR-LT-BEC-100 Beckman 166, DU6/70/600/800 Tungsten Lamp 945672
Philips and Unicam Cat.No Model Description Ref. Philips/Unican

Deuterium Lamps TR-LD-PHU-100 Unicam 4225 D2 Lamp 9551-0023
TR-LD-PHU-101 Unicam UV & Helios series D2 Lamp 9423U/9004B
TR-LD-PHU-105 Unicam 929 939 959 AA Spectrometers D2 Lamp 942339346251
TR-LT-PHU-102 Unicam 5625 6575 8600 8700 8800 Series Tungsten Lamp 942318503021
TR-LT-PHU-103 Unicam Helios Aquamate, Biomate 5, a,b,g,d Tungsten Lamp 9423UV90004E
LKB-Pharmacia Cat.No Model Description Ref. LKB

Deuterium Lamps TR-LD-LKB-100 LKB Pharmacia 2141 4050 4054 Ultraspec D2 Lamp N/A
TR-LD-LKB-101 LKB Productor 2140 D2 Lamp 8010-3135
ECOM Deuterium Lamps Cat.No Model Description Ref. LKB

TR-LD-ECO-100 Ecom LCD 2083 & 2084 Series D2 Lamp 22590000
TR-LD-ECO-101 Ecom Flash Series Opal, Sapphire, Topaz & LCD 2073A D2 Lamp 22590001

Interchim Deuterium
Cat.No Model Description Ref. LKB
Lamps
TR-LD-INT-100 I.Puriflash215 430 450 800 4100 4250-40 4250-250 PF1SP2 D2 Lamp PFS970
PG Instruments Cat.No Model Description Ref. LKB

Deuterium Lamps TR-LD-PGI-100 PG Inst. T70 T80 T92 990 AAS Longlife D2 Lamp N/A
250
Cecil Deuterium Lamps Cat.No Model Description Ref. Cecil
TR-LD-CEC-111 Cecil Series 2 1000 to 9000 CE D2 Lamp 2202-0142
TR-LT-CEC-111 Cecil Series 2 1000 to 9000 CE Tungsten Lamp 2303-0140
Knauer Deuterium Lamps Cat.No Model Description Ref. Knauer

TR-LD-KNA-101 Knauer Wellchrom K2000 K2500 K2501 K2600 K2700 Longlife D2 Lamp A4071
TR-LD-KNA-102 Knauer 8700, 9700, 2151 Longlife D2 Lamp N/A
TR-LD-KNA-103LL Knauer Smartline PDA 2800 2850 K-2800 Longlife D2 Lamp A4447
TR-A5193 Knauer S2250/A5190, S2520 Longlife D2 Lamp A5193
TR-A4073 Knauer K2501 Tungsten Lamp A4073
TR-228-51511-95 Knauer 59200 59201 Xe D2 Lamp A59210
LDC Deuterium Lamps Cat.No Model Description Ref. LDC

TR-LD-LDC-100 LDC 3000 & 4000 Series Spectromonitor I II III D D2 Lamp 108035
TR-LD-LDC-102 LDC 5000 D.A.D. D2 Lamp N/A
TR-LX-LDC-150MO LDC FM4100 Xe Lamp
ACS Deuterium Lamps Cat.No Model Description Ref. ACS

TR-LD-ACS-100 ACS LC 750 11E 12 D2 Lamp N/A
Analytic Jena Deuterium Cat.No Model Description Ref. ACS

Lamps TR-LD-ANJ-100 A.Jena Spekol 1100,1200 Spekord S100/200/50 D2 Lamp 820-60021-0
TR-LD-ANJ-101 A.Jena Spekol 1300/ 1500/200 D2 Lamp 820-60238-0
Biotage Deuterium Cat.No Model Description Ref. ACS
Lamps TR-LD-BTG-100 Biotage Isolera SP1/4 VWL D2 Lamp 09830
GE AKTA Deuterium Cat.No Model Description Ref. ACS

TR-LH-GE-101 GE AKTA UPC-900 Mercury Lamp 28-4042-25
Lamps
TR-LH-GE-102 GE AKTA UV-900 (Monochromator cpl) Xe Lamp 19-1029-35
TR-LH-GE-103 GE AKTA UV-900 (Refurb Exchange) Xe Lamp 19-1029-35
Gynkotek Deuterium Cat.No Model Description Ref. ACS

Lamps TR-LD-GYN-100LL Gynkotek (Dionex) UVD 320 340U 340S 160 170S 170U D2 Lamp 5053.1204
Hach Deuterium Cat.No Model Description Ref. ACS

Lamps TR-LD-HAC-100 Hach Lange DR5000, DR6000 D2 Lamp A23792
Jenway Deuterium Cat.No Model Description Ref. ACS

Lamps TR-I/605016 Jenway 6051 Tungsten Lamp I/605016
TR-012146 Jenway 6270, 6280 & 6285 Xe Lamp 012146
TR-012075 Jenway 6300,6310,6320D,6500,6505,6700,7310 Tungsten Lamp 012075
TR-012094 Jenway 6305 & 6315 Xe Lamp 012094
TR-640508 Jenway 6400 6500 &6505 Longlife D2 Lamp 640508
TR-6705/LA Jenway 6705 & 6715 Xe Lamp 6705/LA
TR-685024 Jenway 6800 & 6850 Tungsten Lamp 685024
TR-12050 Jenway 7200 Tungsten Lamp 12050
TR-7205/SER/LAMP Jenway 7205 & Geneva Bio Xe Lamp 7205/SER/LAMP
TR-I/730545 Jenway 7305 7315 7415/nano, Geneva plus & nano Xe Lamp I/730545
TR-740018 Jenway 7410 & 7600 Tungsten Lamp 740018
Young Lin Deuterium

Cat.No Model Description Ref. ACS
Lamps TR-LD-YNG-101 Young Lin 820 Longlife D2 Lamp N/A
251
HPLC Safety Caps
Dangers and hazards in many laboratories
Reduce pollution
Ecological and sanitary damage can be reduced intensely by
SCAT Safety Caps. Officially accredited testing laboratories verify
a reduction of toxic concentrations in the air amounting to 73%.
Emissions of Acetronile in a test laboratory
during HPLC operation
mg/m3
Safety for HPLC users

Vapours and gases of dangerous liquids can cause damage
to your health and to your environment. Bottles and containers
with unsafe contents always have to be sealed reliably to avoid Open Bottle Bottle with Safety Cap
health hazards and environment pollution.
Avoid shrinkage
Many directives are already regulated by law - in addition, you
should always take care of your health and integrity yourself. Spend less money on chemicals - SCAT Safety Caps avoid
decrease of expensive solvents which otherwise evaporate into
extraction hoods or into the environmental air.
Troubleshooting by SCAT Safety Caps
Solvent shrinkage
• No escape of hazardous vapours Methanol-Water mixture (80/20 after 1 week of testing
Integrated air valve and exhaust filters keep your containers
sealed safely. Bottle with Safety Cap
• No pollution
Containers always remain shut, even during extraction or
Volume in %
filling of liquids.
• No shift of tubing
Tubes remain fixed - no air intake into sensitive equipment
like chromatography systems Bottle without Safety Cap
• Easy container exchange
Safety Caps are freely turnable without twisting tubes.
• No air intake
No interruption of chromatography processes. Time (days)
252
HPLC Safety Caps
253
HPLC Safety Caps
Safety Caps GL 45 - Safe Fluid Supply
Safe venting for pressure equalization during the solvent supply
by integrated air valve. Protection against dangerous solvent
vapours.
Available connectors for tubing:
- 1.6mm (1/16“) OD
- 2.3mm OD
- 3.2mm (1/8“) OD
Cat.No Description
TR-S1-00712 Safety Cap I, (V2.0) GL 45
1 port for 3.2mm (1/8“) OD tubing
TR-S1-00713 Safety Cap II, (V2.0) GL 45
2 ports for 3.2mm (1/8“) OD tubing 1x Blind plug
TR-S1-00714 Safety Cap III, (V2.0) GL 45
TR-S1-00720
3 ports for 3.2mm (1/8”) OD Tubing 2 x Blind Plug
TR-S1-00715 Safety Cap IV, (V2.0) GL 45
TR-S1-00716 Safety Cap V, (V2.0) GL 45
TR-S1-00715 Safety Cap VI, (V2.0) GL 45
TR-S1-00717 Air valve for SafetyCaps service life 6 months
(Box of 1)
TR-S1-00718 Air valve for SafetyCaps service life 6 months
(Box of 10)
TR-S1-00721
PFA fittings for capillaries
Cat.No Description
TR-S1-00157 5x Fitting for 3.2 mm OD Blue
TR-S1-00156 5x Fitting for 3.2 mm OD Red
TR-S1-00159 5x Fitting for 3.2 mm OD Yellow
TR-S1-00151 5x Fitting for 1.6 mm OD Green
TR-S1-00154 5x Fitting for 2.3 mm OD Violet
TR-S1-00082 10 x Blind plug
Starter Kits Thread adapters for SafetyCaps

Cat.No Description Cat.No Description
TR-S1-00720 Starter Kit Supply S1 TR-S1-00233 Adapter GL 38 (f) to GL 45 (m)
content: SafetyCapI 3 u GL45 1 connector 3,2mm OD TR-S1-00235 Adapter GL 40 (f) to GL 45 (m)
Safetycap II 1 unit GL45 2 connectors 3,2 mm OD
Blind plug for tubing connection 1/4”
TR-S1-00721 Starter Kit Supply S2
content: SafetyCapI 3 u GL45 1 connector 3,2mm OD
Safetycap II 1 unit GL45 2 connectors 3,2 mm OD
Blind plug for tubing connection 1/4”
TR-S1-00255 HPLC Safety Set
Content: 4 x Safety Cap III TR-S1-00714;
1x safetywaste Cap TR-S1-00728 4 connectors
1 x Filter Exhaust with indicatoir TR-S1-00724
2x Tube connector 6-8 mm TR-S1-00587 TR-S1-00255
1 x tube connector 9.5-10 Angled TR-S1-00580
254
HPLC Safety Caps
Bottles (Clear Glass) - GL 45 Thread
Round bottles with scale (ml) and screw cap.
Also available with protective covering!
Cat.No Volume Cat.No. with protective Covering
TR-S1-00321 250 ml TR-S1-00322
TR-S1-00325 500 ml TR-S1-00326
TR-S1-00315 1000 ml TR-S1-00313
TR-S1-00319 2000 ml TR-S1-00318
TR-S1-00328 5000 ml TR-S1-00329
Bottles (Brown Glass) - GL 45 Thread

Round bottles (brown glass) with scale (ml) and screw cap.
Cat.No Volume
TR-S1-00320 250 ml
TR-S1-00324 500 ml
TR-S1-00314 1000 ml
Square Bottles (Clear Glass) - GL 45 Thread

Four-cornered bottles for space saving placement. Each bottle
with scale (ml) and screw cap.
Cat.No Volume
TR-S1-00323 250 ml
TR-S1-00327 500 ml
TR-S1-00316 1000 ml
TR-S1-00722 TR-S1-00723
TR-S1-00724 TR-S1-00725
255
HPLC Safety Waste Caps
SafetyWaste Caps
our exhaust filter system absorbs 99% of all volatile substances
evaporating from solvent disposal containers. Cat.No Description
Available connectors for tubing: TR-S1-00149 Exhaust Filter, size S with splash protection and label
- 1.6mm (1/16“) OD TR-S1-00070 4x Exhaust Filter, size S with splash protection and label
- 2.3mm OD
- 3.2mm (1/8“) OD
Cat.No Description
TR-S1-00726 SafetyWaste Cap, GL 45
3 port for 3.2mm (1/8“) OD tubing
TR-S1-00727 SafetyWaste Cap , GL 45
2 ports for 3.2mm (1/8“) OD tubing
1 port for 6-8 mm
TR-S1-00728 SafetyWaste Cap , GL 45
4 ports for 3.2mm (1/8“) OD tubing
SafetyWaste Caps with Safety Funnel
1 port for 6-8 mm
TR-S1-00487 SafetyWaste Cap, S 60/61 For safe disposal of solvent and sample leftovers. simply open the
3 port for 3.2mm (1/8“) OD tubing safety funnel by pressing the locking mechanism.
TR-S1-00486 SafetyWaste Cap, S 60/61
2 ports for 3.2mm (1/8“) OD tubing Cat.No Description
1 port for 6-8 mm TR-S1-00460 SafetyWaste Cap, S 50
TR-S1-00544 SafetyWaste Cap, S 90/91 2 port for 3.2mm (1/8“) OD tubing with funnel
4 ports for 3.2mm (1/8“) OD tubing TR-S1-00483 SafetyWaste Cap, S 55
1 port for 6-8 mm 2 port for 3.2mm (1/8“) OD tubing with funnel
TR-S1-00722 Exhaust Filter, size M with splash protection and label TR-S1-00504 SafetyWaste Cap, S 60/61
TR-S1-00723 2x Exhaust Filter, size M with splash protection and label 2 port for 3.2mm (1/8“) OD tubing with funnel
TR-S1-00724 Exhaust Filter, size M with splash protection andchange TR-S1-00500 SafetyWasteCap S60/61, safety funnel with
indicator shut-off, 2 port 2,3/3,2 mm OD
TR-S1-00725 2x Exhaust Filter, size M with splash protection and
change indicator
256
Sample Injection Rheodyne™ Valves
Complet range of HPLC sample injectors, from Cat.No Description Stator Material
nanoscale to preparative applications.
RH-7725 Dual Mode Analytical Injector Stainless Steel
RH-7725i Dual Mode Analytical Injector with switch Stainless Steel
Rheodyne Injecion Valves for Microscale RH-9725 Dual Mode Analytical Injector PEEK
Applications RH-9725i Dual Mode Analytical Injector wiyh switch PEEK
Model 8125
Manufactured in stainless steel and designed for 1.0 and 2.0 mm

microbore columns. This sample injector can also be used for
analytical columns between 3.0 and 5.0 mm ID.
Maximum recommended injection volume is 200 µl.
Cat.No Description
RH-8125 Microscale sample injector, dual mode, stainless steel
Patented Rheodyne MBB Design:

Flow paths of Model 7725 and 9725 with patented
Rheodyne MBB design
Rheodyne Injecton Valves for Analytical Applications Rotor Seal Shaft End Rotor Seal Groove
Models 7725, 7725i, 9725 and 9725i

Strator Face
Strator Face Seal
All these well recognized valves are manufactured in 316 stainless
Strator
steel. Some specialized features include :
MBB Passage
Pump
Column
• The Rheodyne patented Make-Before-Break (MBB™)
MBB Passage
architecture allows continuous flow between LOAD and
INJECT positions wich greatly reduces transient pressure
Rotor Seal Grooves Rotor Seal Grooves
shocks that disrupt your system.
• Wide 30º portangles offer easier access to fittings using the
Rheodyne wrench (P/N RH-6810).
• Front-end pressure screw makes easy to adjust and maintain
pressure.
• A built-in position sensor switch (“i” versions) provides a “start”
signal to the instrument MBB Passage MBB Passage
• Small diameter internal flow paths assure minimal dispersion.
257
Rheodyne Injection Valves for Preparative • Genuine Rheodyne Parts
• For Popular Rheodyne Valves
Applications
A rotor seal is a polymeric disc that makes a high pressure seal
Models 3725-038, 3725i-038, 3725 and 3725i against the stator in a valve. The seal wears with use and is one of
the few valve parts that may need to be replaced routinely. Stators
need replacement only if the threated ports are damaged or the
sealing surface is scored.
Vespel™ blend Rotor Seals have an operating pH range of 0-10.
Tefzel™ are usable at a pH range from 0 to 14. Some stators can
also be manufactured in stainless steel.
Rotors Vespel
Cat.No Valve Material
RH-7010-039 7010,7000,7040 Vespel
RH-7030-003 7030 Vespel
RH-7060-070 7060,7066 Vespel
RH-7125-047 7125,7725 Vespel
RH-7410-038 7410 Vespel
RH-7413-013 7413 Vespel
RH-8125-038 8125 Vespel
Rotors Tefzel
Cat.No Valve Material
These valves have been designed for high injection volumes and RH-7010-071 7010,7010-087,7000,7040 Tefzel
flow rates up to 100 ml/min. 1/8” or 1/16” tubing can be attached RH-7030-015 7030 Tefzel
usind the right fitting adapter. RH-7060-074 7060,7066,9060 Tefzel
MBB™ patented Rheodyne architecture allows continuous and RH-7125-079 7125,7125-081,7725 Tefzel
stable flow when switching from LOAD to INJECT.
RH-7410-075 7410 Tefzel
Cat.No Description RH-8125-097 8125 Tefzel
RH-3725-038 Preparative Scale Injector, Dual Mode, Stainless Steel RH-9010-051 9010 Tefzel
RH-3725i-038Preparative Scale Injector with switch, Dual Mode, RH-9125-082 9125,9725 Tefzel
Stainless Steel
RH-3725 Preparative Scale Injector, Dual Mode, PEEK Rotors Peek
RH-3725i Preparative Scale Injector with switch, Dual Mode, PEEK Cat.No Valve Material
RH-3030-005 3030,3030-038 Peek
RH-3060-001 3060,3060-038 Peek
RH-3710-008 3000,3000-038,3710,3710-038 Peek
RH-3725-018 3725,3725-038 Peek
RH-7610-011 7610-400,7610-600 Peek
Stators
Cat.No Valve
RH-3060-009 3060
RH-3725-006 3725,3710-038,3000-038
Rheodyne Rotor Seals and Stators RH-3725-085 3725-038,3710-038,3000-038,3030-038
RH-7010-040 7010,7125,7000,7030,7040
RH-7010-066 7125-081,7010-087
RH-7060-039 7060,7066
RH-7410-041 7410,7413
RH-7610-048 7610-600
RH-7725-010 7725
RH-8125-098 8125
RH-9060-016 9060
RH-9125-043 9125,9010,9030,9725
258
Rheodyne RheBuild™ Kits Stainless Steel Sampling Loops for 7125 and 7010 Valves
(Not to be used in 7725 Valves)
Included in each individualized RheBuild Kit are genuine Cat.No. Volume Tubing
Rheodyne parts, tools and instructions to maintain the precision RH-7020 5 µL Loop 0,18 mm (0,007”)ID x 1/16” OD
and performance of your Rheodyne valve. Rheodyne kits
RH-7021 10 µL Loop 0,30 mm (0,012”)ID x 1/16” OD
eliminate individual part ordering at a very convenient price.
RH-7022 20 µL Loop 0,51mm (0,020”)ID x 1/16” OD
RH-7028 2000 µL Loop 1 mm (0,040”)ID x 1/16” OD
Stainless Steel Sampling Loops for 3725-038 and 3725i-038

valves
Cat.No. Volume Tubing
RH-3065-018 2000 µL Loop 2 mm (0,080”)ID x 1/8” OD
RheBuild Kit Model RH-3065-023 10000 µL Loop 2 mm (0,080”)ID x 1/8” OD
RH-3725-999 3725, 3725i, 3725-038, 3735i-038 RH-3065-025 20000 µL Loop 2 mm (0,080”)ID x 1/8” OD
RH-7010-996 Conversion Kit with strator for 7010 model
RH-7010-997 7010 strator included
Stainless Steel Sampling Loops for 7725 and 7725i
RH-7010-999 7010 and 7010 models
(Not to be used in 7125 valves)
RH-7125-999 7125,7126 Cat.No. Volume Tubing
RH-7410-999 7410 RH-7755-020 5 µL Loop 0,18 mm (0,007”)ID x 1/16” OD

RH-7520-999 7520,7526 RH-7755-021 10 µL Loop 0,30 mm (0,012”)ID x 1/16” OD
RH-7725-999 7725,7725i RH-7755-022 20 µL Loop 0,30mm (0,012”)ID x 1/16” OD
RH-9010-999 9010 RH-7755-024 100 µL Loop 0,51 mm (0,020”)ID x 1/16” OD
RH-9725-999 9725,9725i RH-7755-026 500 µL Loop 0,76 mm (0,030”)ID x 1/16” OD
RH-7755-027 1000 µL Loop 0,76 mm (0,030”)ID x 1/16” OD
Sampling Loops for Rheodyne Injection Valves
Available in stainless steel and PEEK. Please note that stainless RH-1877 20000 µL Loop 2 mm (0,080”)ID x 1/8” OD
steel loops are not interchangeable between valve types 7125,
7010 and 7725. Stainless Steel Sampling Loops for 8125 and 7010 Valves
(Please use RH-7755-029 for vol. >50 µl)
RH-8022 20 µL Loop 0,25 mm (0,010”)ID x 0,020” OD

259
PEEK Sampling Loops for 3725 and 3725i Valves Rheodyne Accessories
Accessories for the Injection Port

Cat.No. Description
RH-7012 Stainless Steel Loop Filler Point
RH-9012 PEEK Loop Filler Point
RH-9013 PEEK Needle Port
RH-7125-054 Needle Port Cleaner
RH-3055-018 2000 µL Loop 1,6 mm(0,062”)ID x 1/8” OD RH-9125-076 Suction Needle Adapter (for Model 9725)
RH-3055-019 5000 µL Loop 1,6 mm(0,062”)ID x 1/8” OD
RH-3055-023 10000 µL Loop 2 mm(0,080”)ID x 1/8” OD Mounting Brackets
RH-3055-025 20000 µL Loop 2 mm(0,080”)ID x 1/8” OD
Rheodyne mounting brackets and panels of different shapes and
PEEK Sampling Loops for 9725 and 9010 sizes organize and provide a sturdy support for Rheodyne valves.
Cat.No. Volume Tubing The Ring Stand Mounting Bracket now allows the valves to mount
RH-7755-015 2 µl Loop Internal Loop onto common laboratory equipment.
RH-9055-020 5 µL Loop 0,18 mm(0,007”)ID x 1/16” OD Cat.No. Description
RH-9055-021 10 µL Loop 0,25 mm(0,010”)ID x 1/16” OD RH-7160 Mounting Panel
RH-9055-022 20 µL Loop 0,25 mm(0,010”)ID x 1/16” OD RH-7160-010 Valve Angle Bracket
RH-9055-023 50 µL Loop 0,51 mm(0,020”)ID x 1/16” OD RH-7160-029 Ring Stand Mounting Bracket
RH-7160-10
RH-9055-027 1000 µL Loop 0,76 mm(0,030”)ID x 1/16” OD RH-7160
PEEK Sampling Loops for 7725 and 7725i

RH-7755-015 2 µl Loop Internal Loop
RHEFLEX Fittings RH-7160-029

Cat.No. Description
Other Accessories
RH-6000-083 Nut and Ferrule for 1/8” Loop, 5u.
RH-6000-210 Ferrules for 1/8” loops, 10u. Cat.No. Description
RH-6000-211 Nuts and ferrules for 1/16” Loops, 10u. RH-7161-020 Position Sensor Switch for 7125
UP-P-331 Super Flangeless Nut for RH-1876 and RH-1877 1/8” Loops, 1u. RH-7161-016 Pos. Sensor Switch for 7010, 7410, 7000, 7030, and 7040
UP-P-350X Super Flangeless Ferrule for RH-1876 and RH-1877 Loops, RH-7165 Pos. Sensor Switch for 7250
10u. RH-6810 Rheodyne Wrench
UP-P-654 PEEK adapter for RH-1876 and RH-1877 1/8” Loops, 1u.
RHEFLEX Fittings for PEEK Loops

Cat.No. Description RH-6810
RH-6000-251 PEEK Ferrules for 1/16” Loops, 10u
RH-6000-254 PEEK Nuts and ferrules for 1/16” Loops, 10u
RH-6000-078 PEEK Nuts and Ferrules for 1/8” Loops, 1u
RH-6000-079 PEEK Ferrules for 1/8” Loops, 5u
260
CHROMATOGRAPHY PRODUCTS
261
Teknokroma Contact Data
Our Headquarters:
Teknokroma Analítica S.A.
Camí de Can Calders, 14
ES-08173 Sant Cugat del Vallès (Barcelona)
SPAIN
Phone: +34 936 748 800
Fax: +34 936 752 405
E-mail : [email protected]
Web : www.teknokroma.com
Teknokroma brand products are globally available.

Our qualified distributor network offers a quality service to our customers, in more than 60 countries worldwide.
To find a local Teknokroma representative in your country, please, visit our website:
If your country is not listed in our website, please:
Contact with us:

Export Department
Phone: +34 936 698 650

Fax : +34 936 752 405
E-mail : [email protected]
Web : www.teknokroma.com
GC and HPLC Applications
if you are looking for GC and HPLC aplications please read the following QR code or go to the following link:
http://www.teknokroma.es/en/Aplicaciones.aspx
262 26
263 263
Notes
264

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Index HPLC Chromatography

A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter. HAMILTON PRP-1

mechanism, with inlet and outlet holes of 0.2 mm and first-class

mediterranea sea18 Column 3 µm 134904 1,11 0 2 4 6

Column with Ultrafit™ System + Ultraguard™

The Novacol™ columns, designed and manufactured by

Our Novacol™ columns have added another new feature - the

Lastly, we designed Novacol™ columns to allow you to easily

Novacol™ columns are available in a wide range of standard

Eluyent waste (ml/8 hours)

represents a 80 % saving of the eluyent wasted in HPLC, which Detectability

means that for a standard job in a chromatograph will represent a

Column (mm) Eluyent Waste Detectability

Available also 1 mm internal diameter columns.

Column: Tracer Extrasil

Eluyent: Acetonitrile/H20 65:35

Sample : Oligomeres of Polycarbonate

Charge capacity (mg)

covering practically all kind of functional groups. This simplifies

Besides, a complete range of dimensions of column, from 7.8 mm

Charge capacity (mg) Flow (ml/min)

Analytical Quality Packing

Its high pressure packing ensures a high stability and consequently

Preparative quality packings

The result is an inferior cost of the column and, therefore, in many

All kind of preparative packings and process packings.

(1 cm x 0,32cm) (1 cm x 0,40 cm)

Reference Description Reference Description

(2 cm x 0,32 cm) (2 cm x 0,40 cm)

Reference Description Reference Description

• 100% biocompatible. Guard Column Protein 300 C8 10 x 3.2 mm (5 units) TR-C-160-14

Constructed in PEEK® and packed with de-activated silica: the

Guard Column Cartridges, Biocompatible Product Description Cat.Nbr.

UP-C-280 Reversed Phase C18 3-pk (frit not included)

Guard Column Cartridge Holders, Biocompatible mediterranea™ sea Guard Columns

TR-C-260-1 Prep cartridge ODS (2 units)

1000% The obtained deactivation is shown when we make chromatograms

Nevertheless, these advantages are achieved at the expense of

The mediterranea™ sea18 column also widely surpasses the

Column B - The Competition 0 5 10 15 20

MeOH/H2O H2O Methanol

amitriptyline - activity towards bases Amitriptyline

Until quite recently, silica packings were limited to working between

Using mediterranea™ sea18 packing makes it is possible to work

Eluting the mediterranea™ sea18 column for 78 hours at pH 11.5,

LC-MS Mediterranea™ Sea 18 Columns

Assay by Instituto Químico de Sarriá I.Q.S. (Barcelona)

Chromatographic Conditions Conditions for MS Detection

With Ultra-rapid column separations, total analysis times of less

The combination of 3 mm mediterranea™ sea18 packing with the

characterized by their total inertness, by their wide

extremely low RMS, ensuring that no tube imperfection in the

Ultrafit™ System Efficiency

mediterranea sea18 Column 3 µm 134904 1,11 Ultrafit™

Length Diameter mediterranea Sea18 5 20 0.30 TR-010023

Packing Funct. µm cm cm Cat.Nbr. mediterranea Sea18 5 25 0.30 TR-010024

mediterranea Sea18 5 3 0.40 TR-010007 mediterranea Sea8 5 3 0.30 TR-010391

mediterranea Sea18 5 4 0.40 TR-010008 mediterranea Sea8 5 5 0.30 TR-010392

mediterranea Sea18 5 5 0.40 TR-010009 mediterranea Sea8 5 10 0.30 TR-010393