Heinrich Hermann Robert Koch

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 Heinrich Hermann Robert Koch 

(1843 – 1910) provided remarkable


contributions to the field of microbiology. He was a German general
practitioner and a famous microbiologist.
 He is credited to be one of the founders of the specific field of modern
bacteriology.
 As the founder, he identified the specific causative agents of tuberculosis,
cholera, and anthrax and gave experimental support for the concept of
infectious disease, which included experiments on humans and animals.
 For this he is also regarded as a pioneer of public health, aiding legislation
and changing prevailing attitudes about hygiene to prevent the spread of
various infectious diseases.
 For his work on tuberculosis, he was awarded the Nobel Prize in 1905 in
Physiology or Medicine.

Major Contributions of Robert Koch


 He investigated the anthrax disease cycle in 1876, and studied the bacteria
that cause tuberculosis in 1882 and cholera in 1883. 
 He discovered bacteria such as the anthrax bacilli, tubercle bacilli and cholera
bacilli.
 Koch observed the phenomenon of acquired immunity. 
 He introduced solid media for culture of bacteria. Koch pioneered the use of
agar as a base for culture media. He developed the pour plate method and
was the first to use solid culture media for culture of bacteria.
 Koch also developed media suitable for growing bacteria isolated from the
body. Because of their similarity to body fluids, meat extracts and protein
digests were used as nutrient sources. The result was the development of
nutrient broth and nutrient agar media that are still in wide use today.
 He also introduced methods for isolation of bacteria in pure culture.
 He described hanging drop method for testing motility.
 He introduced staining techniques by using aniline dye.
 He invented the hot air oven and steam sterilizer, and also introduced
methods to find out the efficacy of antiseptics.
 Koch’s Phenomenon: Robert Koch observed that guinea pigs already
infected with tubercle bacilli developed a hypersensitivity reaction when
injected with tubercle bacilli or its protein. Since then, this observation was
called as Koch’s phenomenon.
 The medical applications of biotechnology still heavily depend on the Koch’s
principles of affirming the causes of infectious diseases. 

Contribution to the Germ theory


 Building on the early work of Louis Pasteur and the germ theory of disease,
Robert Koch established the basic scientific requirements used to
demonstrate that each specific disease is caused by a specific microorganism.
 The first direct demonstration of the role of bacteria in causing disease came
from the study of anthrax by the German physician.
 These requirements were based on Koch’s experiments with anthrax isolated
from diseased hosts, and are known as “Koch’s Postulates”.

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