LIKUNI MISSION HOSPITAL LMHL-HEMA-006

LABORATORY BCC3600 OPERATING PROCEDURE

V 1.0
EFECTIVE DATE: AUGUST 2017

Effective Date: Revision 0 Supersedes; None

August 2017

Prepared/Revised by Signature Date

Reviewed by Signature Date

Approved by Signature Date

Distributed to Number of copies

Master file 1

Lab 1

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LMHL-HEMA-BCC3600plus Operating Procedure-006
 LIKUNI MISSION HOSPITAL LMHL-HEMA-006
LABORATORY BCC3600 OPERATING PROCEDURE
V 1.0
EFECTIVE DATE: AUGUST 2017

Document History

Revision Effective Date Changes

Staff Signatures
Signing below indicates that you have read this SOP and understand the material contained in it
Name Signature Date

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LMHL-HEMA-BCC3600plus Operating Procedure-006
 LIKUNI MISSION HOSPITAL LMHL-HEMA-006
LABORATORY BCC3600 OPERATING PROCEDURE
V 1.0
EFECTIVE DATE: AUGUST 2017

1. RESPONSIBLE PERSON: All trained and authorized Laboratory staff.

2. PURPOSE
The purpose of this standard operating procedure is to guide the user to properly, effectively,
and safely run a Full Blood Count (FBC) on clinical samples using the BCC-3600 hematology
analyzer for diagnostic purposes in vitro.

3. PRINCIPLE
The BCC-3600 Auto Hematology Analyzer uses electrical impedance method in counting cells.
This method is based on the measurement of changes in electrical resistance produced by a
particle, which in this case is a blood cell, suspended in a conductive diluent as it passes through
an aperture of known dimension. An electrode is submerged in the liquid on both sides of the
aperture to create an electrical pathway. As each particle passes through the aperture, a
transitory change in the resistance between the electrodes is produced. This change produces a
measurable electrical pulse. The number of pulses generated indicates the number of particles
that passed through the aperture. The amplitude of each pulse is proportional to the volume of
each particle. Each pulse is amplified and compared to the internal reference voltage channels,
which only accepts the pulses of a certain amplitude. Each blood cell has a threshold which will
be referred to and will be counted according to the threshold it falls in.

4. SPECIMEN
EDTA anti-coagulated blood.

5. SAFETY AND ENVIRONMENT

The sample probe is sharp and may contain biohazard material, so avoid contact with the probe
when working around it.
Wear personal protective equipment and follow safe laboratory procedures when handling
samples in the laboratory.

6. MAINTAINANCE
6.1 Daily
Check if there are enough reagents.
Check if the reagents are cloudy and degenerative
Check the tubing’s if there any bends and leakages
Empty the waste container

6.2 Weekly
Soak WBC pool and RBC pool every week.
Click “service” in the main menu interface, then click “Maintenance” to enter maintenance
menu. Click “WBC Pool” in “Sock” area. The WBC pool is soaked will prompt. WBC pool
soaking is finished, click ‘Next” to continue, will prompt after finishing soaking. “The WBC
pool is being rinsed….” will prompt after clicking ‘Next”. Soak is finished after the progress
bar is complete. The operation for soak RBC pool is the same as Soak WBC pool.

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LMHL-HEMA-BCC3600plus Operating Procedure-006
 LIKUNI MISSION HOSPITAL LMHL-HEMA-006
LABORATORY BCC3600 OPERATING PROCEDURE
V 1.0
EFECTIVE DATE: AUGUST 2017

6.3 Monthly
In order to prevent the sampling parts from polluting thereby affecting counting results,
swab should be rinsed every month.
 Wipe the sample probe and swab with cotton dipped in distilled water.
 Click “service” in the main interface, then click “Rinse” to enter rinse interface.
“Rinsing swab….” Will prompt after clicking “swab”. Rinse is finished after the
progress bar is complete.

7. CALIBRATION
You should run calibration programs if it is the first time the analyzer has been used, certain
major component (s) of the analyzer has been changed and if the quality control results indicate
there may be a problem.
To use the calibration program, refer to Operators manual chapter 4 page 4-1 (LMHL-EXT-005).

8. QUALITY CONTROL
Quality control should be run on a daily basis, after running record on the Quality Control Log
(LMHL-HEMA-BC3600plus Quality Control Log-007).
See User manual chapter 5 page 5-3 (LMH-EXT-005) for running of quality control.

9. OPERATION
9.1 Preparation Before Turning on the Instrument
Check if there enough reagents
Check if the reagents are cloudy and degenerative
Check if tubing has any bends
Empty waste container

9.2 Booting
Switch on the main power supply (on the back panel of the equipment) and the system
Loading….” Will show on the screen. The sytem will enter into “Operation interface after
self-check.

9.3 Whole Blood Count

See User Manual Chapter 6 page 6-7 (LMH-EXT-005)

9.4 Predilute Count

See User Manual Chapter 6 page 8-8 (LMHL-EXT-005)

9.5 Shutdown
Click “Shut down” button in the main menu and a prompt box pops up, click “OK” to ensure
the instrument to automatically shut down.

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LMHL-HEMA-BCC3600plus Operating Procedure-006
 LIKUNI MISSION HOSPITAL LMHL-HEMA-006
LABORATORY BCC3600 OPERATING PROCEDURE
V 1.0
EFECTIVE DATE: AUGUST 2017

10. RELATED DOCUMENTS AND FORMS

LMHL-HEMA-BCC3600plus Maintenance Log-008
LMHL-HEMA-BCC3600plus Quality Control Log-007
LMHL-HEMA-BCC3600plus Reagent Log-009

11. REFERENCES
Dirui, BCC3600plus Hematology Analyzer, User Manual.
LMHL-MA-SOP for Writing SOP-001

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LMHL-HEMA-BCC3600plus Operating Procedure-006