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    LPO / Low Power Objective

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    A compound microscope uses multiple lenses or lens systems to magnify specimens that are too small to be seen by the naked eye. It has three main parts - mechanical, magnifying, and illuminating. The mechanical parts include the base, stage, and adjustments for focus. The magnifying parts are the objective and eyepiece lenses. Objectives provide low, high, and oil immersion magnifications. The eyepiece further magnifies the image. Total magnification is calculated by multiplying the objective and eyepiece magnifications. The illuminating parts, such as a mirror and lamp, provide light to view the specimen. Proper care of the microscope involves avoiding liquids, storing it safely, cleaning lenses, and handling it by

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    LPO / Low Power Objective

    Uploaded by

    For Astro
    27 views2 pages
    A compound microscope uses multiple lenses or lens systems to magnify specimens that are too small to be seen by the naked eye. It has three main parts - mechanical, magnifying, and illuminating. The mechanical parts include the base, stage, and adjustments for focus. The magnifying parts are the objective and eyepiece lenses. Objectives provide low, high, and oil immersion magnifications. The eyepiece further magnifies the image. Total magnification is calculated by multiplying the objective and eyepiece magnifications. The illuminating parts, such as a mirror and lamp, provide light to view the specimen. Proper care of the microscope involves avoiding liquids, storing it safely, cleaning lenses, and handling it by

    Original Title

    Microscope

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    A compound microscope uses multiple lenses or lens systems to magnify specimens that are too small to be seen by the naked eye. It has three main parts - mechanical, magnifying, and illuminating. The mechanical parts include the base, stage, and adjustments for focus. The magnifying parts are the objective and eyepiece lenses. Objectives provide low, high, and oil immersion magnifications. The eyepiece further magnifies the image. Total magnification is calculated by multiplying the objective and eyepiece magnifications. The illuminating parts, such as a mirror and lamp, provide light to view the specimen. Proper care of the microscope involves avoiding liquids, storing it safely, cleaning lenses, and handling it by

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    27 views2 pages

    LPO / Low Power Objective

    Uploaded by

    For Astro
    A compound microscope uses multiple lenses or lens systems to magnify specimens that are too small to be seen by the naked eye. It has three main parts - mechanical, magnifying, and illuminating. The mechanical parts include the base, stage, and adjustments for focus. The magnifying parts are the objective and eyepiece lenses. Objectives provide low, high, and oil immersion magnifications. The eyepiece further magnifies the image. Total magnification is calculated by multiplying the objective and eyepiece magnifications. The illuminating parts, such as a mirror and lamp, provide light to view the specimen. Proper care of the microscope involves avoiding liquids, storing it safely, cleaning lenses, and handling it by

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    © All Rights Reserved
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    Download as docx, pdf, or txt
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    Microscope

     A microscope is an instrument used to see objects that are too small for the naked eye.
     The science of investigating small objects using such an instrument is called microscopy.
     Microscopic means invisible to the eye unless aided by a microscope.

    Simple vs. Compound Microscope

    Simple Compound
    Uses single lens Uses set lenses or a lens system

    Compound Microscope
    1. Mechanical Parts – adjustments and support
    2. Magnifying Parts – enlarge the specimen
    3. Illuminating Parts – provide the light

    Mechanical Parts
    a. Base – bottom most portion that supports the entire/lower microscope
    b. Pillar – part above the base that supports the other parts
    c. Inclination Joint – allows for tilting of the microscope for convenience of the user
    d. Arm – curved/slanted part which is held while carrying the microscope
    e. Stage – platform where object to be examined is placed
    f. Stage clips – secures the specimen to the stage
    g. Stage opening
    h. Body tube – attached to the arm and bears the lenses
    i. Draw tube – cylindrical structure on top of the body tube that holds the ocular lenses
    j. Revolving/Rotating Nosepiece – rotating disc where the objectives are attached
    k. Dust shield – lies atop the nosepiece and keeps dust from settling on the objectives
    l. Coarse Adjustment Knob – geared to the body tube which elevates or lowers when rotated bringing
    the object into approximate focus
    m. Fine Adjustment Knob – a smaller knob for delicate focusing bringing the object into perfect focus
    n. Condenser Adjustment Knob – elevates and lowers the condenser to regulate the intensity of light
    o. Iris Diaphragm Lever – lever in front of the condenser and which is moved horizontally to
    open/close the diaphragm

    Illuminating Parts
    a. Mirror – Located beneath the stage and has concave and plane surfaces to gather and direct light in
    order to illuminate the object
    b. Electric Lamp – a built-in illuminator beneath the stage that may eb used if sunlight is not preferred
    or is not available

    Magnifying Parts
    a. Ocular/eyepiece – another set of lens found on top of the body tube which functions to further
    magnify the image produced by the objective lenses. It usually ranges from 5x to 15x.
    b. Objectives – metal cylinders attached below the nosepiece and contains especially ground and
    polished lenses
     LPO / Low Power Objective
    o Gives the lowest magnification, usually 10x
     HPO / High Power Objective
    o Gives higher magnification usually 40x or 43x
     OIO / Oil Immersion Objective
    o Gives the highest magnification, usually 97x or 100x, and is used wet either with
    cedar wood oil or synthetic oil

    Total Magnification
    Magnification = Objective lens x Eyepiece lens

    Ex: What is the total magnification if the objective lens is twenty times (X20) and the eyepiece lens five times
    (X5)?
    Magnification = 20 X 5 = X100
     As magnification increases, detail increases but less of the cell is seen.

    Caring for the Microscope


    1. Do not let any liquids to come in contact with the microscope.
    2. Always store the microscope inside a box after use.
    3. Return the objective lens onto low power after use.
    4. Carry the microscope by the arm.
    5. Use a soft, clean tissue to wipe the lenses

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