Moringa Oleifera Lam: Guidelines For The Conduct of Test For Distinctiveness, Uniformity and Stability On Drumstick
Moringa Oleifera Lam: Guidelines For The Conduct of Test For Distinctiveness, Uniformity and Stability On Drumstick
Moringa Oleifera Lam: Guidelines For The Conduct of Test For Distinctiveness, Uniformity and Stability On Drumstick
On
Drumstick
CONTENTS
I .Subject
1. The Protection of Plant Variety and Farmers Right Authority (PPV & FR) shall decide on the
quantity and quality of the plant material required for testing the variety and when and where it
is to be delivered for registration under Protection of Plant Variety and Farmers Act, 2001.
Applicants submitting such planting material from a country other than India shall make sure
that all customs and quarantine requirements stipulated under relevant national legislations and
regulations are complied with. As a minimum the applicant should submit 30 plants of moringa
or 100 pure seeds for each centre.
2. The plant material supplied should be visibly healthy, not lacking in vigour, nor affected by any
important pest or disease. The seed should be free from pest s without any seed treatment.
3. The plant material /seed should not have undergone any treatment, which would affect the
expression of the characteristics of the variety, unless the competent authorities allow or
request such treatment. If it has been treated, full details of the treatment must be given.
1. The minimum duration of the DUS test shall normally be for at least two pod bearing seasons in
succeeding years.
2. The test should be carried out under the conditions ensuring satisfactory growth for the
expression of the relevant characteristics of the variety and for conduct of the examination.
Each test should include total of 6 plants in 3 replications. In particular, it is essential that the
plants produce a satisfactory crop of pod in each of the two growing seasons.
The design of the tests should be such that plants and parts of plants may be removed for
measurements or counting without prejudice to the observations which must be made up to the end
of the growing cycle. The additional test protocol for special purpose may be established by PPV &
FRA.
1. Locations : two
2. No. of replications : three
3. Treatment unit : Six plants per replication (total 18 plants/location)
4. Spacing : 3*3 m
The characteristics described in the table of characteristics (see section VII ) shall be used for
the testing varieties for their DUS.
1. For the assessment of Distinctness and Stability, observation shall be made on 18 plants or
30 parts taken 6 plants with the exception on the observation on pods which should be made
on at least 20 pods per replication. In the case of parts of plants, the number should be
taken from each of the plant should be three.
2. For the assessment of Uniformity, the population standard of 5% with an acceptance
probability of at least 95% should be applied. In the case of sample size of 18 plants, the
maximum number of off-types allowed would be 1.
3. All observations on the plant and the parts should be made during flowering and pod stage.
4. Time of bloom should be recorded from 6 months after planting to 75% bloom.
5. All the observations on the plant and the branches should be made during flowering and pod
stage.
6. Time of maturity should be recorded from 75% blooming to pods harvest.
7. Observations on the mature pods should be recorded when pods is ready for vegetable
harvest and for after drying of pods.
8. Type of assessment of characteristics as indicated in column of Table VII of characteristics is
as follows.
a) MG: Measurement by a single observation of a group of plants or parts of plants.
b) MS: Measurement by a single observation of individual plants or parts of plant.
c) VG: Visual assessment by a single observation of a group of plants or parts of plants
d) VS: Visual assessment by a single observation of individual plants or parts of plant
V. Grouping of varieties
1. The candidate varieties for DUS testing shall be divided into groups to facilitate the
assessment of Distinctness, characteristics, which are known from experience not to vary, or
to vary only slightly within a variety and which in their various states are fairly evenly
distributed across all varieties in the collection are suitable for grouping purpose.
1. To assess the distinctness, uniformity and stability, the characteristics and their states as
given in the table of characteristics (Section VII) shall be used.
2. Notes (1 to 9) shall be given for each state of expression for different characteristics for the
purpose of electronic data processing.
3. Legend
(*) Characteristics that shall be observed during every growing season on all varieties and
shall always be included in the description of the variety, except when the state of expression
of any of these characters in rendered impossible by a preceding phenological characteristics
or by the environment conditions of the testing region. Under such exceptional situation,
adequate explanation shall be provided.
(+) See Explanation on the table of characteristics in section VIII. It is to be noted that for
certain characteristics, the plant parts on which observations to be taken are given in the
explanation or figure (s) for clarity or not the colour variation.
4. A code number in the sixth column of Table of characteristics indicates the optimum stage for
the observation of each characteristic during growth and development of plant. The relevant
growth stages corresponding to these code numbers are described below:-
a) Observation on plant vigour and habit should be made at the central third of the shoot
during dormant season of adult plants relative to reference cultivars grafted on sweet
seedling root stock.
b) The observations on the leaves should be made on mature leaves from current season’s
shoot.
c) Observations on flowers should be made at the time of full bloom (75% flowering)
d) Observation on pod at vegetable harvest stage and seed should be made after harvest of
pods
e) Observation on pods and seed should be made after harvest of pods.
VII. Table of characteristics:
Spreading
Acute(3) Obtuse(7)
PKM-01:
White
Flowers and
pale green
colour
young fruit
PKM-02:
Pink buds
and red
colour
young fruit
PKM-2: Brown
Mysore-03: Brown coloured
coloured with Oval
with Triangular Shaped seed
Shaped seed
The test guidelines developed by the task force (1/2018) constituted by the PPV & FR Authority for
Drumstick with consultation by Nodal officer, UHS, Bagalkot. Technical inputs also provided by the
PPV & FR Authority.
CONTENTS
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tk ldrs gSaA
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2- izfrd`fr;ksa dh la[;k % rhu
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4- varjky % 3 x 3 eh-
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MG : ikS/kksa ds lewg ;k ikS/ks ds Hkkxksa dh ,dy i;Zo{s k.k }kjk uki
MS : O;fDrxr ikS/kksa ds lewg ;k ikS/ks ds Hkkxksa dh ,dy i;Zo{s k.k }kjk uki
VG : ikS/kksa ds lewgksa ;k ikS/kksa ds Hkkxksa dk ,dy i;Zo{s k.k }kjk n`"VO; ewY;kadu
VS : O;fDrxr ikS/kksa ;k ikS/ks ds Hkkxksa dk ,dy i;Zo{s k.k }kjk n`"VO; ewY;kadu
V. fdLeksa dk lewghdj.k
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a s
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3-
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M-½ Qyh dh vkd`fr ¼xq.k 19½
p½ cht dh vkd`fr ¼xq.k 20½
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djus okyh dksM la[;k,a uhps nh xbZ gSA
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ifÙk;ksa ij fd, tkus pkfg,A
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pkfg,A
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Spreading
uqdhyh eqFkjh
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