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    Devices and Reagents: Burettes, Volumetric Flasks For 100 and 250 ML, Tubes

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    Anuj
    This document provides instructions for students to prepare buffer solutions with specific pH values. It lists the necessary devices, reagents, and buffer solutions that students can choose from for their assignment. The procedure involves calculating volume ratios using pH equations, measuring out the appropriate volumes of initial solutions, mixing them in a flask, testing the pH, diluting the buffer, and recording their results in a table that includes the calculation and volumes used. Questions at the end review buffer systems, calculations, mechanisms of action, and examples of biological buffer systems.

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    Devices and Reagents: Burettes, Volumetric Flasks For 100 and 250 ML, Tubes

    Uploaded by

    Anuj
    30 views2 pages
    This document provides instructions for students to prepare buffer solutions with specific pH values. It lists the necessary devices, reagents, and buffer solutions that students can choose from for their assignment. The procedure involves calculating volume ratios using pH equations, measuring out the appropriate volumes of initial solutions, mixing them in a flask, testing the pH, diluting the buffer, and recording their results in a table that includes the calculation and volumes used. Questions at the end review buffer systems, calculations, mechanisms of action, and examples of biological buffer systems.

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    YEs

    Original Title

    Laboratory work 6

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    This document provides instructions for students to prepare buffer solutions with specific pH values. It lists the necessary devices, reagents, and buffer solutions that students can choose from for their assignment. The procedure involves calculating volume ratios using pH equations, measuring out the appropriate volumes of initial solutions, mixing them in a flask, testing the pH, diluting the buffer, and recording their results in a table that includes the calculation and volumes used. Questions at the end review buffer systems, calculations, mechanisms of action, and examples of biological buffer systems.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    30 views2 pages

    Devices and Reagents: Burettes, Volumetric Flasks For 100 and 250 ML, Tubes

    Uploaded by

    Anuj
    This document provides instructions for students to prepare buffer solutions with specific pH values. It lists the necessary devices, reagents, and buffer solutions that students can choose from for their assignment. The procedure involves calculating volume ratios using pH equations, measuring out the appropriate volumes of initial solutions, mixing them in a flask, testing the pH, diluting the buffer, and recording their results in a table that includes the calculation and volumes used. Questions at the end review buffer systems, calculations, mechanisms of action, and examples of biological buffer systems.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Laboratory work 6

    Preparation of buffer solutions with a given pH


    Devices and reagents: burettes, volumetric flasks for 100 and 250 ml, tubes,
    pipettes, chemical glasses for 50 ml, funnels, universal indicator paper; 0.1N
    solutions of acetic acid and sodium hydroxide, 0.2M solutions of ammonium
    hydroxide and hydrochloric acid.
    Experimental part:
    Procedure:
    1. Get an assignment from the teacher (see table 1 for options)
    Table 1
    № of Buffer solution pH Initial solution Initial solution
    option volume, ml 1 2
    1 100 4 0,1N CH3COOH 0,1N NaOH
    2 100 4,25 0,1N CH3COOH 0,1N NaOH
    3 100 4,75 0,1N CH3COOH 0,1N NaOH
    4 100 5 0,1N CH3COOH 0,1N NaOH
    5 100 5,25 0,1N CH3COOH 0,1N NaOH
    6 100 9 0,2M NH4OH 0,2M HCl
    7 100 9,25 0,2M NH4OH 0,2M HCl
    8 100 9,5 0,2M NH4OH 0,2M HCl
    9 100 10 0,2M NH4OH 0,2M HCl
    10 100 10,5 0,2M NH4OH 0,2M HCl

    2. Calculate the required ratio of the volume of the initial solutions, using the
    formula for calculating the pH of buffer solutions.
    3. Calculate the absolute values of the volumes of the initial solutions required for
    the preparation of 100 ml of the buffer mixture.
    4. Select a smaller volume solution. Rinse the burette, then fill it to zero. Pour the
    required volume from the burette in the appropriate volumetric flask.
    5. Pour the second solution through the funnel into the flask just below the mark.
    Then bring to the mark, adding solution from the pipette. Close the flask with a
    stopper and stir.
    6. Pour about 5ml of the buffer mixture into a cup or tube. Determine the pH using
    universal litmus paper. Compare the color with the standard color on the scale.
    7. Pour 2 ml of buffer solution and 8 ml of water in a test tube. Calculate the
    dilution of buffer solution. Determine the pH after dilution with universal indicator
    paper.
    8. Fill in table 2. Write the calculation of the quantitative composition of the
    mixture.
    Table 2
    The equations pH calculation The volume of The volume of pH by
    of equilibrium solution 1 solution 2 indicators
    in the system

    9. Wash the glassware.

    Contents of the report:


    1. Theme and purpose.
    2. Tables, calculations.
    3. Conclusions on the work done.
    Questions and tasks
    1. Buffer systems, their classifications.
    2. Calculation of рН of acid and basic buffer solutions.
    3. Mechanism of action of buffer systems.
    4. Buffer capacity.
    5. Basic buffer systems of the organism. Acidosis. Alkalosis.
    6. Hydrocarbonate buffer system, mechanism of action.
    7. Hemoglobin buffer system, mechanism of action.
    8. Phosphate buffer system, mechanism of action.
    9. Protein buffer system, mechanism of action.

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