Lab - nr.3. Insulina Si Glucoza PDF

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Exercise 4: Endocrine System Physiology: Activity 2: Plasma Glucose, Insulin, and Diabetes Mellitus Lab Report

Pre-lab Quiz Results


You have not completed the Pre-lab Quiz.

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Experiment Results
Predict Question:
Predict Question: To measure the amount of plasma glucose in a patient sample
Your answer : c. a blood sample must be collected from an artery in the arm.

Stop & Think Questions:


Why does the color in the tubes vary?
You correctly answered: c. The tubes contain different amounts of glucose.

Experiment Data:

Tube Optical Density Glucose (mg/deciliter)


Part 1 1 0.30 30
2 0.50 60
3 0.60 90
4 0.80 120
5 1.00 150
Part 2 1 0.73 106
2 0.79 114
3 0.89 134
4 0.83 122
5 0.96 145

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Post-lab Quiz Results
You scored 33% by answering 2 out of 6 questions correctly.

1. A male patient has had successive fasting plasma glucose readings of 115, 110, and 122 mg/dl. The healthcare provider
will inform him that
Your answer: a. he has developed diabetes and needs to adopt lifestyle changes.
Correct answer: c. he appears to have impairment or borderline impairment of insulin-mediated glucose uptake by his cells.

2. To obtain an accurate spectrophotometric measurement of the glucose concentration in the sample


Your answer: c. an enzymatic color reagent is added that digests and, thus, removes blood clots.
Correct answer: d. heparin is added to prevent blood clots.

3. In the spectrophotometric assay used in this experiment, the __________ as the glucose concentration in the sample
increases.
Your answer: c. optical density remains constant
Correct answer: b. optical density increases

4. A female patient has had successive fasting plasma glucose readings of 130, 140, and 128 mg/dl. The healthcare
provider will inform her that
You correctly answered: a. she has developed diabetes.

5. To maintain plasma glucose homeostasis


Your answer: c. cells will become unresponsive to insulin if plasma glucose levels are chronically low.
Correct answer: d. insulin-mediated transport of glucose into cells acts as negative feedback when plasma glucose levels
rise.

6. A laboratory technician withdraws a blood sample from a vein in your upper arm knowing that
You correctly answered: b. the plasma glucose concentration will be the same in both the arm vein and the index finger.

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Review Sheet Results
1. What is a glucose standard curve, and why did you need to obtain one for this experiment? Did you correctly predict
how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve?
You did not answer this question.

2. Which patient(s) had glucose reading(s) in the diabetic range? Can you say with certainty whether each of these
patients has type 1 or type 2 diabetes? Why or why not?
You did not answer this question.

3. Describe the diagnosis for patient 3, who was also pregnant at the time of this assay.
You did not answer this question.

4. Which patient(s) had normal glucose reading(s)?


You did not answer this question.

5. What are some lifestyle choices these patients with normal plasma glucose readings might recommend to the borderline
impaired patients?
You did not answer this question.

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