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    Biochemistry

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    RAKESHBH
    This document provides information on various biochemistry tests including the test principle, reagents used, potential interferents, and wavelength. It lists the tests for glucose, blood urea nitrogen, creatinine, total and direct bilirubin, uric acid, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, and triglycerides. It also provides explanations and recommended actions for potential errors that could occur with the tests.

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    Biochemistry

    Uploaded by

    RAKESHBH
    65 views3 pages
    This document provides information on various biochemistry tests including the test principle, reagents used, potential interferents, and wavelength. It lists the tests for glucose, blood urea nitrogen, creatinine, total and direct bilirubin, uric acid, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, and triglycerides. It also provides explanations and recommended actions for potential errors that could occur with the tests.

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    This document provides information on various biochemistry tests including the test principle, reagents used, potential interferents, and wavelength. It lists the tests for glucose, blood urea nitrogen, creatinine, total and direct bilirubin, uric acid, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, and triglycerides. It also provides explanations and recommended actions for potential errors that could occur with the tests.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    65 views3 pages

    Biochemistry

    Uploaded by

    RAKESHBH
    This document provides information on various biochemistry tests including the test principle, reagents used, potential interferents, and wavelength. It lists the tests for glucose, blood urea nitrogen, creatinine, total and direct bilirubin, uric acid, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, and triglycerides. It also provides explanations and recommended actions for potential errors that could occur with the tests.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 3

    BIOCHEMISTRY

    TEST PRINCIPLE REAGENTS INTERFERENCE WAVE


    LENGTH
    GLU Hexokinase with ATP converts G-6PD, Hb(500) 20 L, 340, 383
    GLU to glucose-6-phosphate. G- ATP,NADP BIL(20) 12 L, end point
    6-PD then oxidizes it and TG(600) 10 H
    simultaneously converts NADP
    TO NADPH
    BUN Urease splits urea to ammonia Urease, 340 rate
    and co2, the former reacts with NADPH, GLDP
    alpha keto gluterate to form
    amine reductively and
    simultaneously oxidizes
    NADPH TO NADP
    Crea Picric acid in strong alkaline sol Lithium picrate, 510,710
    reacts with creatinine to form Naoh,
    red chromophore
    TBIL Diazo sulfinilic acid comp is Sodium nitrite, 540,600
    formed by sod. Nitrite and hcl. Sulfinilic
    sulfinilic acid. The insoluble Bil acid,
    is solubilized by buffer. buffer,caffine,
    sod benzoate,
    disod EDTA
    DBIL Diazo sulfinilic acid comp is Sodium nitrite
    formed by sod. Nitrite and sulfonilic acid
    sulfinilic acid hcl
    URCA Uricase converts uric acid to Buffer TG at 600mg/dl 293,700
    allantoin which is not absorbed stabilizers, decreases by end point
    at 293nm. Disappearance of uric uricase 0.2mg
    acid causes change in
    absorbance
    TP Cu ++ forms complex with Hb(500mg) 540,700
    peptide bonds in alkaline sol to H,dextran L.
    form blue sol. Tartrate prevents BIL L,
    ppt of Cu(oh)2
    ALB Bromocresol purple(BCP) forms BCP dye, CMPF in sera of 540,600,
    complex with albumin at ph 4.9 acetate buffer, CRF pts give false 700
    in presence of a stabilizing surfactant low alb
    agent.
    AST Asparate transferase catalyzes LDH,MDH, 340,700
    transamination from L-asparate NADH
    to ketoglutrate forming L- Ketoglutrate
    glutamate and oxaloacetate. asparatic acid,
    Oxaloacetate is reduced to buffer
    malate by MDH and NADPH is
    oxidised
    ALT ALT catalyzes transamination of LDH, NADH. 340,700
    L-alanine to K Glutrate Kg,buffewr,alan
    formaing L-glutamate and ine
    pyruvate. Pyruvate is reduced to
    lactate by LDH and NADH is
    oxidized
    TG Lipase splits TG into glycerol NAD, Lipase, 340,383
    and fatty acids. GDH oxidises GDH, bich.
    glycerol and reduces NAD buffer,k2co3
    CHOL Cholesterol esterase free
    cholesterol from cho esters.
    HDL
    CA
    CHEMISTRY TROUBLESHOOTING
    ERROR Explanation action
    Abnormal Abs reading was outside the limits Rerun, if persists run QC, if QC is OK
    assay set by result monitor function problem is with sample else remove flex,
    confirm and reinsert
    Abnormal Foam, bubbles, turbidity in the Align the sample and reagent probe
    reaction reaction mixture in cuvette
    Aborted test Either by user or instrument Load/run errors
    Absorbance The mA at the measured wavelength Rerun if error recurs it is due to optical
    exceeds 2000 interference
    a) Assay Result>assay range autodiluted and Manually dilute and rerun
    rng/dilu rerun but still> range
    b) low ‘A’ Absorbance Lower than limit set Same start with smallest dilution
    error
    c) high ‘A’ Absorbance higher than limit set -do- +(urine drugs of use should be
    centrifuged & not diluted)
    d) sust deplet’n Kinetic check exceeds the limit for As in b
    that method
    Arithmetic Error in result calculation change in If on pt sample the conc is very high or
    abs was less than C0, or > C0+C1 low. If QC also is shifted low change 2500
    ul reagent flush syringe tip, use new well
    a) Above assay -do- Manually dilute and rerun
    range
    b) Below assay -do- Ensure enough sample rerun after dil 1:1
    range with std . 50% recovery of std confirms low
    or no activity
    Assay Above or below assay range LOW- dil with std or QC compare with
    Sampleconc=(dilresult*mix vol )- expected result to exclude system
    (stdvol*stdconc)/sample vol malfunction
    HIGH: dilute if permitted and rerun
    Calibration
    expired
    Hemoglobin Hemolysis greater than 250mg/dl Dbil is not checked for hgb error
    errorTBIL/DBI
    L
    Hi/Lo High or low result No action
    Measurement Insignificant software timing error Rerun. If persists problem in measurement
    system
    Temperature Cuvette temp is out of range

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