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    BOT 20 Laboratory Exercise 1

    Uploaded by

    Lukas Blanco
    This document describes an experiment on seed germination and seedling growth under different conditions. It tests the effect of: 1. Gas composition by comparing germination in ordinary air vs. nitrogen gas. 2. Temperature by comparing germination at room temperature vs. low and high temperatures. 3. pH levels by comparing germination at pH 3, 7, and 11. It also tests the effect of osmotic concentration by comparing germination in distilled water and NaCl solutions of increasing concentration. The document records germination rates and seedling growth measurements for each treatment and poses study questions about the purpose and results.

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    BOT 20 Laboratory Exercise 1

    Uploaded by

    Lukas Blanco
    109 views1 page
    This document describes an experiment on seed germination and seedling growth under different conditions. It tests the effect of: 1. Gas composition by comparing germination in ordinary air vs. nitrogen gas. 2. Temperature by comparing germination at room temperature vs. low and high temperatures. 3. pH levels by comparing germination at pH 3, 7, and 11. It also tests the effect of osmotic concentration by comparing germination in distilled water and NaCl solutions of increasing concentration. The document records germination rates and seedling growth measurements for each treatment and poses study questions about the purpose and results.

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    © © All Rights Reserved

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    This document describes an experiment on seed germination and seedling growth under different conditions. It tests the effect of: 1. Gas composition by comparing germination in ordinary air vs. nitrogen gas. 2. Temperature by comparing germination at room temperature vs. low and high temperatures. 3. pH levels by comparing germination at pH 3, 7, and 11. It also tests the effect of osmotic concentration by comparing germination in distilled water and NaCl solutions of increasing concentration. The document records germination rates and seedling growth measurements for each treatment and poses study questions about the purpose and results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    109 views1 page

    BOT 20 Laboratory Exercise 1

    Uploaded by

    Lukas Blanco
    This document describes an experiment on seed germination and seedling growth under different conditions. It tests the effect of: 1. Gas composition by comparing germination in ordinary air vs. nitrogen gas. 2. Temperature by comparing germination at room temperature vs. low and high temperatures. 3. pH levels by comparing germination at pH 3, 7, and 11. It also tests the effect of osmotic concentration by comparing germination in distilled water and NaCl solutions of increasing concentration. The document records germination rates and seedling growth measurements for each treatment and poses study questions about the purpose and results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    BOT 20 Fundamentals of Plant Physiology

    Exercise 1 Seed Germination and Seedling Growth


    Species Used ____________________________________ D. Effect of Osmotic Concentration
    Mean length of
    A. Effect of Gas Composition Treatment Germination (%) the hypocotyl-
    Mean length of root axis (cm)
    Treatment Germination (%) the hypocotyl- distilled water
    root axis (cm) (control)
    Ordinary air 0.5% NaCl
    (control) 1.0% NaCl
    Nitrogen gas 1.5% NaCl
    (anaerobic) Discussion:
    Carbon __________________________________________________
    dioxide __________________________________________________
    Discussion:
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________
    __________________________________________________ Study Questions:
    __________________________________________________
    1. What is the purpose of displacing air with nitrogen gas?
    B. Effect of Temperature ______________________________________________
    Mean length of ______________________________________________
    Treatment Germination (%) the hypocotyl- ______________________________________________
    root axis (cm) ______________________________________________
    Room Temperature ______________________________________________
    (°C) ______________________________________________
    Low Temperature ______________________________________________
    (refrigerator, °C)
    ______________________________________________
    High Temperature
    ______________________________________________
    (oven, °C)
    ______________________________________________
    Discussion:
    2. Why is germination usually low in soils that contain high
    __________________________________________________
    amount of partially decomposed organic matter?
    __________________________________________________
    ______________________________________________
    __________________________________________________
    ______________________________________________
    __________________________________________________
    ______________________________________________
    __________________________________________________
    ______________________________________________
    __________________________________________________
    ______________________________________________
    __________________________________________________
    ______________________________________________
    C. Effect of pH ______________________________________________
    Mean length of ______________________________________________
    Treatment Germination (%) the hypocotyl- ______________________________________________
    root axis (cm) ______________________________________________
    distilled water 3. Does light affect seed germination? What are
    (control) photodormant seeds?
    pH 3 ______________________________________________
    pH 7 ______________________________________________
    pH 11 ______________________________________________
    Discussion: ______________________________________________
    __________________________________________________ ______________________________________________
    __________________________________________________ ______________________________________________
    __________________________________________________ ______________________________________________
    __________________________________________________ ______________________________________________
    __________________________________________________ ______________________________________________
    __________________________________________________ ______________________________________________
    __________________________________________________

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