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    Colorimetric Reaction

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    ALEXANDRA MARIE BUNQUIN
    This document outlines several common biochemical tests used to detect different amino acids and functional groups present in proteins and peptides. The tests are described along with the target analyte or functional group detected, required reagents, and expected color change results for a positive or negative test outcome. The tests can identify the presence of peptide bonds, alpha amino acids, aromatic amino acid side chains, phenol groups in tyrosine, indole groups in tryptophan, arginine, sulfur containing amino acids, amides in asparagine and glutamine, and aromatic amino acids like tyrosine and histidine.

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    Colorimetric Reaction

    Uploaded by

    ALEXANDRA MARIE BUNQUIN
    17 views1 page
    This document outlines several common biochemical tests used to detect different amino acids and functional groups present in proteins and peptides. The tests are described along with the target analyte or functional group detected, required reagents, and expected color change results for a positive or negative test outcome. The tests can identify the presence of peptide bonds, alpha amino acids, aromatic amino acid side chains, phenol groups in tyrosine, indole groups in tryptophan, arginine, sulfur containing amino acids, amides in asparagine and glutamine, and aromatic amino acids like tyrosine and histidine.

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    This document outlines several common biochemical tests used to detect different amino acids and functional groups present in proteins and peptides. The tests are described along with the target analyte or functional group detected, required reagents, and expected color change results for a positive or negative test outcome. The tests can identify the presence of peptide bonds, alpha amino acids, aromatic amino acid side chains, phenol groups in tyrosine, indole groups in tryptophan, arginine, sulfur containing amino acids, amides in asparagine and glutamine, and aromatic amino acids like tyrosine and histidine.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    17 views1 page

    Colorimetric Reaction

    Uploaded by

    ALEXANDRA MARIE BUNQUIN
    This document outlines several common biochemical tests used to detect different amino acids and functional groups present in proteins and peptides. The tests are described along with the target analyte or functional group detected, required reagents, and expected color change results for a positive or negative test outcome. The tests can identify the presence of peptide bonds, alpha amino acids, aromatic amino acid side chains, phenol groups in tyrosine, indole groups in tryptophan, arginine, sulfur containing amino acids, amides in asparagine and glutamine, and aromatic amino acids like tyrosine and histidine.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 1

    TEST FUNCTION REAGENT RESULT

    Biuret Test Detect the presence of peptide bonds 2.5M NaOH (+) Violet solution
    0.1M CuSO4
    Ninhydrin Test Typical test for alpha amino acid 0.1% ninhydrin solution (+) Blue violet
    (-) Yellow
    Xanthoproteic Test Detects side chains of aromatic amino acids concentrated HNO3 (+) Yellow to orange
    concentrated NaOH
    Millon’s Test Phenol group in tyrosine Millon’s reagent  mercuric nitrate; (+) Red precipitate
    concentrated HNO3
    Hopkins-Cole Test Indole group of tryptophan Hopkins-Cole reagent (+) Violet blue
    Concentrated H2SO4
    Sakaguchi Test Detecting the presence of arginine 10% NaOH (+) Red solution
    0.02% naphthol solution
    2% NaOBr
    Nitroprusside Test Presence of sulfur containing amino acids 3M NaOH (+) Red
    2% nitroprusside solution
    Fohl’s Test Sulfur amino acids in cysteine and methionine 30% NaOH (+) Dark (black/brown) sediments
    5% Pb(CH3COO)2
    Test for Amides Detect R-groups of asparagine and glutamine 20% NaOH (+) Red to blue
    Pauly’s Test Detect aromatic amino acid in tyrosine or histidine Diazo reagent  1% sulfanilic acid; (+) Red
    5% NaNo2 solution
    10% Na2CO3

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