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    Qualitative Test Test For Reagent Result/S

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    Thonieroce Apryle Jey Morelos
    This document describes several qualitative tests used to detect different amino acids and peptide bonds in proteins. It lists the name of each test, what biochemical it detects, the reagents used, and the expected color change result. Some of the major tests mentioned are the Biuret test for peptide bonds, Ninhydrin test for alpha-amino acids, Xanthoproteic test for aromatic amino acids, and Millon's test for amino acids containing phenol groups like tyrosine.

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    Qualitative Test Test For Reagent Result/S

    Uploaded by

    Thonieroce Apryle Jey Morelos
    24 views2 pages
    This document describes several qualitative tests used to detect different amino acids and peptide bonds in proteins. It lists the name of each test, what biochemical it detects, the reagents used, and the expected color change result. Some of the major tests mentioned are the Biuret test for peptide bonds, Ninhydrin test for alpha-amino acids, Xanthoproteic test for aromatic amino acids, and Millon's test for amino acids containing phenol groups like tyrosine.

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    Qualitatives Tests

    Original Title

    Qualitative Tests

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    This document describes several qualitative tests used to detect different amino acids and peptide bonds in proteins. It lists the name of each test, what biochemical it detects, the reagents used, and the expected color change result. Some of the major tests mentioned are the Biuret test for peptide bonds, Ninhydrin test for alpha-amino acids, Xanthoproteic test for aromatic amino acids, and Millon's test for amino acids containing phenol groups like tyrosine.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    24 views2 pages

    Qualitative Test Test For Reagent Result/S

    Uploaded by

    Thonieroce Apryle Jey Morelos
    This document describes several qualitative tests used to detect different amino acids and peptide bonds in proteins. It lists the name of each test, what biochemical it detects, the reagents used, and the expected color change result. Some of the major tests mentioned are the Biuret test for peptide bonds, Ninhydrin test for alpha-amino acids, Xanthoproteic test for aromatic amino acids, and Millon's test for amino acids containing phenol groups like tyrosine.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    QUALITATIVE TEST TEST FOR REAGENT RESULT/S

    - Biuret Reagent:
    General test for the Hydrated CuSO4
    BIURET TEST presence of peptide Potassium Hydroxide Blue-Violet Color
    bonds Solution
    Potassium Sodium
    Tartrate

    NINHYDRIN TEST For alpha-amino acids - Ninhydrin Solution Blue Violet Color

    To detects the side


    chains of aromatic
    XANTHOPROTEIC TEST amino acids or to - Conc. HNO3 Clear Yellow
    differentiate aromatic - Conc. NaOH Color
    amino acids from
    other amino acids

    For detecting amino


    acid containing - Millon’s Reagent Brick Red Color
    MILLON’S TEST phenol group
    (tyrosine)

    For detecting amino - Hopkins-Cole Purple Ring


    HOPKINS-COLE TEST acid tryptophan Reagent Coloration at the
    present in protein - Conc. H2SO4 interface

    For detecting - 10% NaOH


    presence of - 0.02% Naphthol Red Color
    SAKAGUCHI TEST guanidium group Solution
    (arginine) - 2% NaOBr

    For determining the


    presence of cysteine, - 3M NaOH
    NITROPRUSSIDE TEST the only amino acid - 2% Nitroprusside Red Color
    containing a sulfhydryl Solution
    group (-SH)
    For detecting the - 30% NaOH
    FOHL’S TEST presence of sulfur - 5% Pb(CH3COO)2 Appearance of
    containing amino Black sediment
    acids

    For detecting Red litmus paper


    TEST FOR AMIDES asparagine and - 20% NaOH should turn to blue
    glutamine

    For detecting - Diazo Reagent:


    PAULY’S TEST aromatic amino acid 1% Sulfanilic Acid & Red Color
    tyrosine and histidine 5% NaNO2 Solution
    - 10% Na2CO3

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