Scribd Logo
Upload

Welcome to Scribd!

  • 83 views

    Determining Refractive Index of Glass Beads: Test Procedure For

    Uploaded by

    gerntrash2
    This document provides the test procedure for determining the refractive index of glass beads using a liquid immersion method. Key steps include placing glass beads on a microscope slide and examining them under a microscope using different refractive index liquids. The correct refractive index liquid is identified when the beads are almost invisible when in perfect focus and have a blurred outline when moving the focus up or down. The procedure distinguishes between situations where the liquid has an index higher or lower than the beads to determine the next liquid to use.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Determining Refractive Index of Glass Beads: Test Procedure For

    Uploaded by

    gerntrash2
    83 views2 pages
    This document provides the test procedure for determining the refractive index of glass beads using a liquid immersion method. Key steps include placing glass beads on a microscope slide and examining them under a microscope using different refractive index liquids. The correct refractive index liquid is identified when the beads are almost invisible when in perfect focus and have a blurred outline when moving the focus up or down. The procedure distinguishes between situations where the liquid has an index higher or lower than the beads to determine the next liquid to use.

    Original Title

    ctm822.pdf

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides the test procedure for determining the refractive index of glass beads using a liquid immersion method. Key steps include placing glass beads on a microscope slide and examining them under a microscope using different refractive index liquids. The correct refractive index liquid is identified when the beads are almost invisible when in perfect focus and have a blurred outline when moving the focus up or down. The procedure distinguishes between situations where the liquid has an index higher or lower than the beads to determine the next liquid to use.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    83 views2 pages

    Determining Refractive Index of Glass Beads: Test Procedure For

    Uploaded by

    gerntrash2
    This document provides the test procedure for determining the refractive index of glass beads using a liquid immersion method. Key steps include placing glass beads on a microscope slide and examining them under a microscope using different refractive index liquids. The correct refractive index liquid is identified when the beads are almost invisible when in perfect focus and have a blurred outline when moving the focus up or down. The procedure distinguishes between situations where the liquid has an index higher or lower than the beads to determine the next liquid to use.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    DETERMINING REFRACTIVE INDEX OF GLASS BEADS TXDOT DESIGNATION: TEX-822-B

    Test Procedure for

    DETERMINING REFRACTIVE INDEX OF GLASS


    BEADS

    TxDOT Designation: Tex-822-B


    Effective Date: August 1999

    1. SCOPE

    1.1 This method details the procedure and equipment used for determining the refractive
    index of glass beads by the liquid immersion method.

    1.2 The values given in parentheses (if provided) are not standard and may not be exact
    mathematical conversions. Use each system of units separately. Combining values from
    the two systems may result in nonconformance with the standard.

    2. APPARATUS

    2.1 Microscope.

    2.2 Microscope slide, of medium-thickness glass, having spherical concavities that are
    ground and polished, with approximate diameter of 18 mm (0.75 in.) and depth of
    0.8 mm (1/32 in.)

    2.3 Microscope illuminator, with iris diaphragm.

    2.4 Set of refractive index liquids, covering the refractive index range of the beads under
    examination.

    3. PRINCIPLE OF PROCEDURE

    3.1 This test uses the principle of light bending as it passes from a medium with one index of
    refraction to another medium with a different index of refraction. This bending is not
    apparent if the different mediums have the same index of refraction.

    3.2 Three situations are possible when determining the index of refraction of glass beads by
    the liquid immersion method:
     the refractive index liquid used is too high
     the refractive index liquid used is too low
     the refractive index liquid used is correct (i.e., has the same index as the beads).

    3.3 The procedure includes descriptions of each of these situations.

    CONSTRUCTION DIVISION 1–2 LAST REVIEWED: OCTOBER 2014


    DETERMINING REFRACTIVE INDEX OF GLASS BEADS TXDOT DESIGNATION: TEX-822-B

    4. PROCEDURE

    4.1 Place a small quantity of beads (enough to cover an area about 3 mm [1/8 in.] in diameter
    with a single layer) in the concavity of the glass slide.

    4.2 Select a liquid with a known refractive index that is near the suspected index of the
    beads.

    4.3 Place one drop of this liquid in the concavity completely covering the beads.

    4.4 Adjust the light from the microscope illuminator so that a dim light illuminates a limited
    portion of the bead-covered area from below.

    4.5 Focus the microscope on the beads. Vary the focus slowly, first in one direction then in
    the other, while closely observing the appearance of individual beads through the
    microscope.

    4.5.1 If, while adjusting the focus in a direction that decreases the distance between the
    microscope stage and the objective lens, a dark ring appears on the circumference of a
    bead and light concentrates in the center, the liquid used has an index of refraction higher
    than that of the bead. Adjustment of the focus in the opposite direction shows the bead
    blurring, with no ring and a bright center spot appearing. When this situation is present,
    remove the slide, wipe off the liquid and beads, place another layer of beads in the slide
    concavity, and use a lower refractive index liquid.

    4.5.2 If, while adjusting the focus in a direction that increases the distance between the
    microscope stage and the objective lens, a dark ring appears on the circumference of a
    bead and light concentrates in the center, the liquid used has an index of refraction lower
    than that of the bead. Adjustment of the focus in the opposite direction shows the bead
    blurring, with no ring and a bright center spot appearing. When this situation is present,
    remove the slide, wipe off the liquid and beads, place another layer of beads in the slide
    concavity, and use a higher refractive index liquid.

    4.6 Continue this evaluation and selection of liquids with different indices of refraction until
    finding the correct one. When the correct liquid is used, the beads will be almost invisible
    when perfectly in focus and will have a blurred outline when moving the focus in either
    direction.

    CONSTRUCTION DIVISION 2–2 LAST REVIEWED: OCTOBER 2014

    You might also like