Automated ELISA System: Operator's Manual

DSX™ Automated ELISA System
Operator’s Manual
IMPORTANT
Please read carefully before using the DSX
Revision History
Revision Date:
June 2000
December 2001
March 2002
Part No. 91000060

This manual is published by Thermo Labsystems.
Questions or comments regarding the content of this manual can be
directed to the address below or to your supplier.
Thermo Labsystems
14340 Sullyfield Circle
Chantilly, VA 20151-1683 USA
®
Microtiter is a registered trademark of Thermo Labsystems.
 2001 This document is the copyright of Thermo Labsystems and must

not be copied or reproduced in any form without prior consent.
Thermo Labsystems reserves the right to make technical improvements to
this equipment and documentation without prior notice as part of a
continuous program of product development. This manual supersedes all
previous editions.
Table of Contents
Table of Contents
Table of Contents ......................................................................................................... i

About this Manual........................................................................................................iii
Chapter 1 Overview.................................................................................................... 1
Introduction .......................................................................................................... 1
Samples.......................................................................................................... 1
Reagents ........................................................................................................ 2
Pipetting.......................................................................................................... 2
Dilutions.......................................................................................................... 2
Incubation ....................................................................................................... 2
Washing ......................................................................................................... 2
Detection ........................................................................................................ 2
Features............................................................................................................... 3
Chapter 2 Description................................................................................................. 5
Hardware Components........................................................................................ 5
Indicator Light ................................................................................................. 5
System Cover ................................................................................................. 6
Workspace ..................................................................................................... 7
Ambient Drawer.............................................................................................. 9
Barcode Readers.......................................................................................... 10
Plate Holders ................................................................................................ 11
Pipette Module.............................................................................................. 12
Sample Mixing During Dilution ..................................................................... 13
Pre-Dilution................................................................................................... 14
Incubator Modules ........................................................................................ 15
Wash Module ............................................................................................... 16
Absorbance Module...................................................................................... 23
Software............................................................................................................. 27
DSX Configuration........................................................................................ 28
Consumables Management ......................................................................... 29
Assay Definition............................................................................................ 30
Worklist Creation .......................................................................................... 32
Worklist Execution........................................................................................ 32
Data Analysis................................................................................................ 32
Results Reporting ......................................................................................... 32
Data Backup ................................................................................................. 32
Required But Not Provided........................................................................... 33
Computer...................................................................................................... 33
Specifications..................................................................................................... 34
Warning Labels.................................................................................................. 36
Chapter 3 Installation................................................................................................ 37
Unpacking.......................................................................................................... 37
Materials Provided........................................................................................ 37
Positioning the Instrument ................................................................................. 38
Connecting the Computer System..................................................................... 39
Loading Revelation™ Software ......................................................................... 40
Creating a Shortcut Icon............................................................................... 41
Connecting the DSX Power Cord ...................................................................... 42
DSX™ System Operator’s Manual i

Table of Contents
Starting the System ........................................................................................... 43

Self Tests .......................................................................................................... 44
Chapter 4 Preparing the System for Use ................................................................. 45
Configuring the DSX.......................................................................................... 45
Editing Washer Plate Settings........................................................................... 46
Selecting the Results Font................................................................................. 48
Setting Options .................................................................................................. 49
Changing the Password .................................................................................... 50
Specifying Levey Jennings Criteria.................................................................... 51
Specifying Consumables ................................................................................... 52
Filling the Wash Buffer Containers.................................................................... 53
Connecting the Waste Container ...................................................................... 54
Barcode Label Suggestions............................................................................... 55
Chapter 5 Defining an Assay..................................................................................... 57
Creating a New Assay ....................................................................................... 57
Modifying an Assay............................................................................................ 58
Chapter 6 Creating a Worklist .................................................................................. 59
Creating a Worklist ............................................................................................ 59
Modifying a Worklist .......................................................................................... 63
Chapter 7 Running a Worklist .................................................................................. 65
The Run Timeline Screen.................................................................................. 65
Timeline Screen Toolbar .............................................................................. 66
Right Mouse Menu ....................................................................................... 67
Reviewing Run Settings .................................................................................... 68
Checking Consumables and Fluids................................................................... 72
Starting the Run................................................................................................. 73
Monitoring Run Status ....................................................................................... 75
Modifying the Worklist During a Run ................................................................. 76
Chapter 8 Safety ...................................................................................................... 77
Safety Suggestions............................................................................................ 77
Chapter 9 Service and Maintenance ........................................................................ 79
Routine Maintenance Procedures ..................................................................... 79
Cleaning and Decontamination ......................................................................... 81
Removing a Module........................................................................................... 82
Replacing the Absorbance Module Lamp ......................................................... 83
Replacing an Absorbance Module Filter............................................................ 85
Replacing the Tubing......................................................................................... 87
Cleaning the Wash Head .................................................................................. 89
Chapter 10 Service................................................................................................... 91
Returning a Module for Service ......................................................................... 91
Limited Warranty ............................................................................................... 93
Warranty and Special Provisions ................................................................. 93
Thermo Labsystems Contact Information ......................................................... 94
Index.......................................................................................................................... 95
ii DSX™ System Operator’s Manual

Introduction
About this Manual

This manual has been written for laboratory technicians and provides
detailed instructions for using the DSX™ Automated ELISA System.
This manual gives you the information needed to:
• Install the DSX™
• Set up the DSX™ to suit your specific application requirements
• Understand the DSX™ menus
• Run assays using the DSX™
• Create or modify assays
• Perform required preventive maintenance
• Service the DSX™
• Review safety precautions
DSX™ System Operator’s Manual iii

Introduction
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iv DSX™ System Operator’s Manual

Introduction
Chapter 1 Overview
Introduction
The DSX™ Automated ELISA System (Figure 1) is a computer-controlled
microplate processing system that fully automates ELISA assays. The DSX
System automates the sample distribution, incubation, reagent addition,
washing and detection phases of microplate assays. It is intended for use in
clinical, research and industrial laboratories.
The DSX is an automated system that is useful for medium throughput,
multiple assay applications. The system is designed to process specimens
in a continuous flow from sample to result. Operator attendance is normally
not required once a run has started.
Samples
A run can contain up to 96 samples. Serum and/or plasma specimens are
generally used for clinical testing, although other fluids such as urine or
spinal fluid can be run. Specimens containing large particulate matter, such
as stool, tissue homogenates or culture media, can also be used. It is
recommended that these specimens are processed through a mesh filter
prior to loading onto the system to assure sample homogeneity and
pipetting precision.
Sample IDs can be manually entered by the operator or they can be read
automatically from barcode labels on the sample tubes.
A wide variety of standards or control samples can also be run. Standards
or controls are loaded onto a separate rack that contains up to 33 standard/
control tubes. The tubes are available from your supplier.
Figure 1. The DSX™ Automated ELISA System
DSX™ System Operator’s Manual 1

Introduction
Reagents
The DSX System utilizes a single reagent rack that contains up to 24
bottles. The required reagent bottles are available from your supplier.
Pipetting
Pipetting of samples, standards/controls and reagents is performed using
custom-designed disposable pipette tips to assure pipetting precision and
eliminate possible cross-contamination. Reagent tips pipette from 25 µL to
1 mL of reagents, and sample tips pipette from 5 µL to 300 µL of samples
or standards/controls.
Dilutions
The DSX System will perform single-stage or multi-stage dilutions of
sample. Dilutions ranging from 1:2 to 1:40,000 can be performed, using
deep-well dilution plates on the system for dilutions above 1:60.
Incubation
Up to four temperature-controlled plate incubators may be present. Each
incubator can be set at a temperature ranging from ambient plus 7 °C to
50 °C, and each plate can be shaken during incubation. The particular
incubator modules that are used during a worklist, the temperature of each
incubator, and the shake parameters are automatically set by the system
when the worklist is created.
Washing
Eight wells in one column of a 96-well plate can be washed simultaneously.
Washing protocols can be defined so that all of the columns are washed in
the same manner, or different wash cycles can be applied to specified
columns on a plate.
A wide variety of user-defined wash protocols can be programmed on the
system. In addition, different plate types can be accommodated.
Detection
During operation, each microplate is automatically transferred to the
absorbance module at the appropriate time. The optical densities of the
wells specified during assay definition are read, the various calculations (for
example, blanking, QC of raw data, threshold or curve fitting) are applied,
and the calculated results for the microplate are reported.
2 DSX™ System Operator’s Manual

Features
Features
The DSX™ Automated ELISA System has a number of performance and
convenience features. These are summarized below:
• Different assays can be run on the same plate

• ESP™ - Electronic Signature Pipetting for detection of gross
pipetting errors
• Automatic reading of Sample IDs and microplate IDs from barcode
labels
• Endpoint data analysis to perform qualitative and quantitative data
reduction
• Less than 10 second reading time (using single wavelength)
• On-board self diagnostics
• Selection of up to six filters
• Single and dual wavelength reading modes
• Easily removable incubator, wash, and absorbance modules for
servicing
• Small footprint
• A variety of wash protocols can be programmed
• A variety of plate types can be programmed
• Liquid level sensing on Waste Container and Wash Buffer
Containers
• Quick dispense
• Aspirating/pipetting speed can be changed for viscous liquids

Features

Hardware Components
Chapter 2 Description
Hardware Components
Locations of the principal hardware components of the DSX™ Automated
ELISA System are shown in Figure 2.
Indicator Light
The system contains an indicator light (Figure 3) that is illuminated
whenever the system power is ON.
CAUTION: Power is on to the system and to the incubator

heaters whenever the indicator light is illuminated. A
thermal hazard is present.
Wash Buffer/ Pipette Work System Absorbance Wash Incubator

Dispense Containers Module Area Cover Module Module Modules
Ambient
Drawer
Waste Tip
Liquid Waste
Container
Container
Sample Tube
and Microplate
Barcode Reader
Figure 2. Location of Principal Hardware Components

Hardware Components
System Cover
The system cover encloses the workspace and pipette module. The cover
must be closed during operation to prevent the pipette module from
accidentally contacting an operator or bystander. An electrical interlock
prevents operation of the pipette module and unattended operation of the
laser barcode reader when the system cover is open.
CAUTION: The electrical interlock for the system cover

prevents accidental contact with the pipette module and/or
robotic arm. Never disable the interlock unless instructed to
do so by Thermo Labsystems personnel.
CAUTION: The system cover attenuates the beam of the

laser barcode reader, preventing inadvertent exposure.
Never disable the interlock for the system cover unless
instructed to do so by Thermo Labsystems personnel.
To open the cover, lift the handle until the cover is in the upright position
(Figure 3). The cover will remain in this position until it is closed. To close
the cover, push down on the handle until the cover is fully closed and
locked. The system cover rests on the cover stop when it is fully closed.
CAUTION: Pinching hazard. Be sure that your hands and

fingers are clear of the cover stop when closing the system
cover.
Handle
System Cover
Indicator
Light
Cover
Stop
Figure 3. System Cover

Hardware Components
Workspace
Samples, reagents, standards and controls, and consumables are loaded
onto the workspace. Their locations are shown in Figure 4.
Sample tubes are contained in seven sample racks. Each sample rack
contains sample 14 tubes and, although up to 98 samples can be contained
on the system at one time, 96 tubes are used in routine operation. Sample
tubes of uniform size must be used.
Note: The particular size sample tube that is used and the position
of the barcode label on the sample tube are specified during
configuration of the DSX (see page 45).
The sample racks are contained in two sample caddies. A specific type of
sample caddy will be used on your system.
Note: The DSX workspace file for the sample caddy is specified
during configuration of the DSX (see page 45).
Standards or controls are contained in the control rack, which contains up

to 33 standard/control tubes. A specific 1.5-mL tube is required for
standards or controls. The tubes can be obtained from your supplier.
Sample Dilution Reagent Reagent Tip Waste

Racks Plate Rack Tip Rack Chute
Sample
Tip Racks
Reagent
Tip Rack
Dilution
Plate
Control
Rack
Figure 4. The Workspace

Hardware Components
Sample tips used for pipetting samples and standards/controls are

contained in four sample tip racks. Each rack contains 108 sample tips,
and a total of 432 sample tips can be loaded on the system. A specific
sample tip is required and can be obtained from your supplier.
Reagent tips used for pipetting reagents are contained in a reagent tip
rack that contains 41 reagent tips. A specific reagent tip is required and can
be obtained from your supplier.
Reagent bottles are contained on the reagent rack, which contains up to
24 reagent bottles that hold approximately 25 mL. A specific reagent bottle
is required and can be obtained from your supplier.
Two deep-well dilution plates can also be loaded. The dilution plates are
used for predilution of a sample before the sample is pipetted into a
microplate well.
After a sample tip or reagent tip has been used, the pipette module moves
over the tip waste chute and ejects the tip. The tip waste chute directs the
used sample tip or reagent tip into the waste container.

Hardware Components
Ambient Drawer
The ambient drawer is used to store microplates when room temperature
incubation is required. The ambient drawer will extend into the work area
during pipetting.
When setting up a worklist, the plate carrier is extended from the ambient
drawer (Figure 5), plate holders (see the following page) are placed in
each of the ambient drawer positions, and the required microplates are
placed onto the plate holders.
Note: The microplate positions are numbered from 1 to 4. Refer to

page 59 for a summary of the procedure to prepare a worklist.
Position 1 Position 2 Position 3 Position 4
Figure 5. The Ambient Drawer

Hardware Components
Barcode Readers
Two barcode readers (Figure 7) are available to read barcode labels on
sample tubes and microplates. The horizontal barcode reader is an LED
barcode scanner. The vertical barcode reader is a CDRH Class II laser
barcode scanner.
CAUTION: The vertical barcode scanner has a maximum

radiated power output of 1.0 milliwatt. Do not stare into the
beam of the vertical barcode scanner. Obey the warning
labels (shown below) that are attached to the system in the
vicinity of the vertical barcode scanner.
(Located on the Front (Located Next to the

of the System Cover) Barcode Reader)
Either the horizontal barcode reader or the vertical barcode reader can be
used. The barcode reader used, the barcode symbology, and the location
coordinates of barcode labels on sample tubes and microplates are
specified during configuration of the DSX (see page 45).
Vertical Barcode
Reader
Warning Label
Horizontal
Barcode Reader
Figure 6. Barcode Readers

Hardware Components
Plate Holders
Plate holders (Figure 7) allow the pipette module to transfer a microplate
between modules or to position a microplate in front of the barcode reader
while the barcode label of the microplate is read.
Each plate holder has a pickup feature which the pipette module uses to
transfer a microplate.
Note: Whenever this manual refers to a microplate, it is implied

that the microplate is seated in a plate holder.
Gripping Positioning
Slot Pins
Figure 7. Plate Holder

Hardware Components
Pipette Module
The pipette module is used to transfer microplates, to hold microplates or
sample racks in front of the barcode reader, to pipette samples, controls
and standards, dispense reagents, and to perform dilutions. The pipette
module travels in the x-, y- and z- directions to access the samples,
controls, reagents, microplates and consumables on the workspace. The
pipette module is shown in Figure 8.
The pipette module has the following functions:
Function Purpose
Microplate The microplate handler transfers a microplate

Handling between the ambient drawer, an incubator module,
the wash module and the absorbance module by
gripping the microplate holder, moving it to its new
location, and releasing it. The microplate handler
also positions a microplate in front of the barcode
reader so that the barcode can be read.
Sample Rack The sample rack handler positions a sample rack
Handling in front of the barcode reader so that the barcode
labels of the sample tubes can be read.
Pipetting The pipettor pipettes samples, standards and
(automatic controls (using disposable sample pipette tips) and
liquid level reagents (using disposable reagent pipette tips).
sensor) Each pipette tip is automatically discarded into the
waste bin after use. A new pipette tip is obtained
from the sample tip rack or reagent tip rack when it
is needed.
Tip Ejection Verifies that a used tip was ejected before obtaining
Detection a new tip.
Pipette Module
Microplate and
Sample Rack
Holder Clamp and
Tip Ejector
Pipettor
Figure 8. The Pipette Module

Hardware Components
The pipetting profile specifies the rate at which fluids are aspirated or
dispensed from the pipette tip. Separate pipetting profiles ranging from
1 to 5 can be specified for any fluid except wash buffer.
IMPORTANT: For any pipetting device, fluid properties

such as viscosity and surface tension can influence the
aspirate and dispense accuracy. Thermo Labsystems
calibrate and verify all pipettes using an aqueous
calibration fluid which when used with special calibration
apparatus provides traceability to a NIST standard and
gravimetric method.
It is important to note that the calibration and traceability is
related only to the calibration fluid and not to any other
fluid type. For most applications the differences in
delivered volumes for common fluid types and the
calibration fluid used in manufacturing are insignificant.
However, at low sample dispense volumes (<20ul) these
may become more apparent.
It is the burden of the user to perform the validation
studies necessary to assure proper assay performance.
CAUTION: Changing the pipetting profile can affect

accuracy and precision of an assay.
The pipetting profile used for samples is specified during definition of

worklist runtime parameters. The pipetting profiles used for standards and
controls are specified during definition of assay operations. The default
pipetting profile for samples, standards, and controls is 4.
Note: Refer to page 68 for the procedure to define worklist

runtime parameters. Refer to page 30 for the procedure to
define assay operations.
The pipetting system of the DSX™ Automated ELISA System includes

ESP™ (Electronic Signature Pipetting) software for automatic detection of
gross pipetting inaccuracies.
Sample Mixing During Dilution

The user can program up to 9 dispense/aspirate mix cycles during dilution
steps to achieve optimal mixing of samples during dilution.

Hardware Components
Pre-Dilution
The DSX™ Automated ELISA System can perform a pre-dilution of
samples before they are assayed. Pre-dilution can be performed in a single
stage in standard microplates or in two stages using deep-well microplates.
The system can be programmed to add diluent to a plate before or after the
sample is added.
The dilution modes are described below:
Dilution Mode Description
Microplate Ratios of sample to diluent range from 11:1 to

Dilution 1:59 (e.g. 5 µL sample combined with 295 µL of
diluent to yield a 1:59 dilution.
Deep-well Plate Ratios of sample to diluent range from 2:1 to
Dilution 1:190 (e.g. 5 µL sample combined with 950 µL
of diluent to yield a 1:190 dilution.
plus Microplate 1:11,210 (e.g. 5 µL sample combined with
Dilution 950 µL of diluent in a deep-well to form a new
sample from which 5 µL is combined with
295 µL of diluent in a microplate to yield a
1:11,210 dilution.
plus Deep-well 1:36,100 (e.g. 5 µL sample combined with
Plate Dilution 950 µL of diluent in a deep-well to form a new
sample from which 5 µL is combined with
950 µL of diluent in another deep-well to yield a
1:36,100 dilution.

Hardware Components
Incubator Modules
Microplates are incubated and shaken in the incubator modules. A
maximum of four incubator modules are present, so that different
microplates can be incubated at different temperatures with or without
shaking. Incubation temperature and shake duration are specified during
definition of an assay.
Note: Refer to page 30 for a summary of the procedures to define

an assay. A microplate can also be incubated at ambient
temperature without shaking by allowing it to remain in the ambient
drawer for a specified period of time.
The particular incubator modules that are used during a worklist and the
temperature and shaking of each incubator are automatically set by the
system when the worklist is created. Processing of the worklist will not
commence until the temperature of each required incubator module is at the
correct value.
Note: The incubator modules are labelled as 1, 2, 3 and 4 and are

designated as such in the Revelation™ software. The incubator
modules will control microplate temperature at any specified
temperature ranging from ambient plus 7 °C to 50 °C.
Incubator
Modules
4
1 Ambient
Drawer
2
Figure 9. Incubator Modules

Hardware Components
Wash Module
The well contents of a microplate are washed in the wash module
(Figure 10). The wash module is designed to wash all 8 wells in one column
of an 8 x 12 microplate simultaneously. The washing protocol can be
defined to wash partially filled plates containing complete columns.
Different user-defined wash protocols can be contained on the system. In
addition, the system can be configured with different plate types so that the
wash head positions for each plate type can be specified. The system can
accommodate flat-bottom, C-bottom, U-bottom and V-bottom types of
microplates.
Note: Refer to page 18 for a summary of the wash protocol

alternatives.
Wash Head
The wash head contains two sets of wash pins. The shorter pins (the
dispense pins) dispense fluid and the longer pins (the aspirate pins)
aspirate fluid. The aspirate pins and the dispense pins are closely spaced
so that fluid can be aspirated from and dispensed into wells at the same
time.
The wash pins are fixed to the wash head. During operation, the wash head
assembly is lowered to insert the wash pins into the wells or raised to
remove the wash pins from the wells. Lowering the wash head assembly
allows the well contents to be aspirated or a bottom wash to be performed.
Raising the wash head assembly allows the wash head to be moved so
another column can be washed or so the wells can be filled.
Plate Wash Dispense/Aspirate

Drawer Head Pins
Figure 10. Wash Module

Hardware Components
Wash Head Positions

The vertical positions that the wash head can assume (Figure 11) are
described below. Each wash head position can be specified by the user to
within 0.1 mm.
Note: Wash head positions for various plate types are specified
during consumables management. See page 29 for additional
information.
Wash Head Position Description
Dispense Height Defines the position at which fluid will be

dispensed. While it is often desirable to set
this height just above the top of the well in
order to form a positive meniscus, the user
should ensure that an overflow does not
occur.
Top of Well Positions the aspiration pins so they are
aligned with the top of the well. At this
position, the liquid meniscus is removed
from the top of the well.
Aspiration Height Positions the aspiration pins at the bottom
of the well so that the contents of the well
can be completely aspirated.
Sweep Height Raises the aspiration pins slightly above
the Aspiration Height (see above) so that
the aspiration pins can be moved back and
forth in the well while the fluid is being
aspirated without the danger of scratching
the bottom of the plate.
Bottom Wash Height Lowers the wash head to this height during
dispense so that the force of the
dispensed fluid can wash the bottom of the
wells.
Sweep Stroke Defines the horizontal motion used during
a sweep operation to fully aspirate well
contents.
Note: Select No Sweep for sweep mode and disable bottom

washing whenever a C-bottom, U-bottom or V-bottom plate is
being used.

Hardware Components
Dispense
Aspirate
Microplate
Figure 11. Position of the Wash Pins
Wash Protocol Operations

A wash protocol consists of a series of Purge, Move, Soak, Aspirate,
Dispense and Fill operations. Purge, Move and Soak can be carried out in
any sequence. Aspirate, Dispense and Fill can only be carried out within a
Move operation, and there cannot be a Move within a Move.
Each of these operations is summarized below:
Operation Description
Purge Dispenses fluid from the dispense wash pins while

the wash head is positioned over the purge tray. A
purge is usually carried out at the beginning of a
wash protocol or at the end of the day to rinse the
dispense wash pins and remove air bubbles.
Move Performs Aspirate, Dispense, Fill and/or Soak
operations on specified strips of the Microplate.
Aspirate Removes the contents of a well by positioning the
wash pins at the aspiration height in the well and
aspirating the liquid from the wells.
A sweep may also be performed during Aspirate.
Dispense Dispenses a specified amount of fluid into the wells
after aspirating the contents of the wells. If a
bottom wash is specified, the wash head is then
lowered to the bottom wash position so that fluid
will be aspirated from the bottom of the wells while
fluid is being dispensed.
Soak The contents of the wells are allowed to equilibrate
for the specified number of seconds.
Fill The wells are filled with a specified amount of fluid.

Hardware Components
Wash Head Sweep Modes
Sweep modes specify whether the aspiration tip moves from side-to-side
during aspiration. Five sweep modes can be used:
• No sweep
• Always sweep
• Sweep on last cycle only
• Always Super Sweep
• Super sweep last cycle only
Note: Select No Sweep for sweep mode and disable bottom

washing whenever a C-bottom, U-bottom or V-bottom plate is
being used.
Plate Drawer
The plate drawer holds the microplate in a known position so that the wash
pins are precisely positioned in the wells during various wash protocol
operations. The plate drawer allows linear shaking of the plate.
Wash Buffer Containers

Up to four different washing and/or dispensing reagents are contained on
the system in wash buffer containers. The wash buffer containers are
located at the front of the instrument (Figure 12).
Each container contains up to two liters of wash buffer. Dispensing of wash
buffer from a container is controlled by a submersible pump in the wash
container, a dispense valve above the wash bottle, and a dispense valve
located near the wash head. The specified wash buffer is dispensed into
wells whenever a Dispense or Fill operation is specified in the wash
protocol.
Each wash buffer container must contain at least 500 mL of wash buffer in
order to be used.
Note: The particular wash buffer that is used and the Dispense,
Fill or Purge operations are defined during assay definition. See
page 57 for a summary of the procedures for assay definition.
A quick connect fitting and a level sensor/pump connector allow easy

removal of a wash buffer container from the system (Figure 13). Disconnect
the wash line by pressing on the metal tab of the quick connect fitting and
gently pulling up on the wash line to remove it. Disconnect the level sensor
and pump connector by pulling it out of the connector socket.
Fill (or empty) a wash buffer container using the filler cap at the rear of the
container.

Hardware Components
Dispense Dispense Dispense Dispense

Valve A Valve B Valve C Valve D
Wash Buffer Wash Buffer Wash Buffer Wash Buffer

Container A Container B Container C Container D
Figure 12. Wash Buffer Containers and Wash Buffer Dispense Valves
Level Sensor and

Pump Connector
Filler Cap
Quick Connect
Fitting
Figure 13. Wash Buffer Container Details

Hardware Components
Waste Containers
Fluid that is removed during purging and washing is collected in the liquid
waste container. Used sample and reagent pipette tips are disposed into
the tip waste container. Both waste containers are located at the front of
the instrument (Figure 14).
Tip Waste Liquid Waste

Container Container
Figure 14. Waste Containers
The liquid waste container (Figure 15) holds up to eight liters of waste. A
level sensor alerts the operator when the liquid waste container is full.
Two quick connect fittings connect the liquid waste and vacuum lines to
the liquid waste container. Disconnect each fitting by pressing on the metal
tab of the quick connect fitting and gently pulling on the line to remove it.
Disconnect the level sensor connector by pulling it out of the connector
socket. Empty the liquid waste container by removing the waste cap at the
front of the container.
Note: Be sure that the waste cap is securely tightened. Otherwise,

a vacuum leak will cause the software to create a vacuum error
condition.
Note: A 10% (v/v) solution of household bleach in water is

recommended as a disinfectant. If the container is full, add
undiluted bleach directly to the container. For example, if the
container has approximately seven liters of waste in it, add 700 mL
of undiluted household bleach. Mix the bleach with the contents
before discarding.

Hardware Components
Level Sensor Manifold Vacuum Waste

Connector Connector Connector Cap
Figure 15. Liquid Waste Container

Hardware Components
Absorbance Module
The absorbance module measures the optical density (OD) of the final
reaction mixture in the microplate wells. The wavelength mode that is used
and the wavelength(s) at which the optical density is measured are
specified during assay definition.
Note: Procedures for specifying the wavelength mode and the

wavelength(s) at which the optical density is measured are defined
during assay definition. See page 57 for a summary of the
procedures for assay definition.
Note: The current version of Revelation™ software supports

endpoint reactions.
During operation, each microplate is automatically transferred to the

absorbance module at the appropriate time. The optical densities of the
wells specified during assay definition are read, the various calculations (for
example, Blanking, QC Raw Data, Threshold or Curve Fitting) are applied,
and the calculated results for the microplate are reported.
Note: Procedures for specifying the manner in which assay results

are calculated and reported are defined during assay definition.
See page 57 for a summary of the procedures for assay definition.
The location of the absorbance module is shown in Figure 16.
Absorbance
Module
Ambient
Drawer
Figure 16. Absorbance Module

Hardware Components
Single and Dual Wavelength Modes

The Reader is able to take readings in two different modes:
• Single--using one test wavelength
• Dual--using one reference wavelength and one test wavelength
The Single wavelength mode is sufficient for most applications.
The Dual wavelength mode can be used if it is necessary to reduce errors
caused by dirt and scratches on the bottom of the wells.
The choice of test and reference wavelengths for the Dual Wavelength
mode depends on the particular enzyme/substrate system being tested.
However, the following rules should usually be followed:
1. The test wavelength (λt) should be at or near the maximum

absorbance of the reaction product.
2. The reference wavelength (λr) should lie outside the

absorbance band of the system but not far removed.
The Reader subtracts the absorbance at the reference wavelength (λr) from
the absorbance at the test wavelength (λt) to minimize the effect of
systematic errors.
If a test requires particular precision, you may specify test and reference
filters of the same wavelength. The Reader will average the ODs produced
using each filter, giving a more precise result.
405 nm λt λr 690 nm
FILTER WAVELENGTH
Figure 17. Dual Wavelength Selection

Hardware Components
Blanking
The Reader lets you subtract a reference value from all the ODs. It
automatically uses air as a reference, but for certain applications other
reference levels may be more appropriate.
For example, you may want to eliminate the absorbance of a reagent
solution from the test result. The Reader can hold the OD of this reagent
solution in memory and subtract it from all subsequently read ODs.
Blanks may be single wells or an average of wells.

Hardware Components

Software
Software
Revelation™ software is used to control the DSX microplate processing
system. The software automates the sample distribution, incubation,
reagent addition, washing and detection phases of microplate assays. It
also provides the user interface for configuration of the instrument and
management of consumables (Figure 18).
The software includes an extensive menu of assay definition options that
allow you to customize the readings, calculations, QC checks and results
format for an assay.
Additional information about the software can be found in the DSX Online
Operator’s Manual, accessed by selecting the Help menu.
DSX Configuration
Consumables
Management
Assay Definition
Worklist Creation
Data Verification
Worklist Execution
Data Analysis
Results Reporting
Figure 18. Revelation™ Software Overview

Software
DSX Configuration
The DSX System can be configured for particular uses in your laboratory.
Refer to Chapter 4 (Preparing the System for Use) for instructions.
The configuration options are summarized below:
Option Description
Configure System Define the COM port.

(Setup DSX) Specify the workspace file that is used.
Designate whether LIMS support is enabled,
and specify the LIMS installation directory and
data directory.
Specify the absorbance filters that are used
and the absorbance data conversion limits.
Specify the barcode reader used, the barcode
symbology, and the location coordinates of
barcode labels on sample tubes and
microplates.
Specify whether the washer float switch is
enabled.
Specify whether a debug log is maintained, and
designate the path of the well fill verification
directory.
Results Font Select the font to be used for printed results
reports.
Options Create short-cut buttons.
Select the colors for different well types.
Set default directories.
Enter the laboratory name and address to be
printed on results reports.
System Password Change the default password in Revelation™
software.
Levey Jennings Specify the assay, well type, and plates for
which a Levey Jennings analysis is to be
performed.

Software
Consumables Management
A database of all consumables and fluids is maintained in the software.
Once a consumable or fluid is defined in the database, it can be selected
from a drop-down list during definition of an assay.
Consumables and fluids are defined using the Tools menu. The fluids and
consumables that are defined and the information that is entered for each
are summarized below:
Consumable Information Entered
Washer Fluids Define the fluids that are used for washing and
purging.
Sample Tubes Define sample tubes and their specific
dimensions.
Reagent/Diluent Define reagents and diluents, and specify
Fluids whether they are time-sensitive. If they are,
specify the maximum on-system use time.
Specify the pipette profile used when aspirating
and dispensing a reagent or diluent. The profile
should be set to 4 unless otherwise required.
Standard/ Define standards and controls, and specify

Control Fluids whether they are time-sensitive. If they are,
specify the maximum on-system use time.
Specify the pipette profile used when aspirating
and dispensing a standard or control.
Washer Plates Specify the height of the washer head for
various washing positions (for example, well top
height, dispense height and wash height) for
each type of microplate that is used.
Specify sweep modes that can be used during
washing and their parameters.
Specify whether bottom washing can be used
during washing.

Software
Assay Definition
The reagents, standards and controls that are used and the dilution, wash
and incubation procedures for an assay are specified during assay
definition. These options are defined using the Operations menu.
Arrangement of well types on the microplate, reading options, and
procedures to be used for calculations, QC checks and reporting when
running a test are also specified during assay definition. These options are
defined using the Data Reduction menu.
The assay definition options are summarized below:
Option Purpose
Operations Menu:
Pipette Samples/ Specify plate templates.
Standards/ Specify sample, standard or control volumes.
Controls Specify pipetting techniques and tip usage.
Specify dilutions.
Dispense Fluid Specify reagent volumes.
Wash Plate Specify plate types, wash methods and wash
buffer volumes.
Specify details for the Purge and Clean cycles.
Specify details for the Soak and Shake options.
Incubate Plate Specify incubation time, temperature and
shaking.
Data Reduction Menu:

Data Reduction Displays the Data Reduction dialog boxes in
Wizard the order they appear on the menu, starting
with Assay Title.
Assay Title Specify assay title, author, code and password.
Reader Control Specify assay wavelength(s), wavelength mode
and shake.
Template Specify the use (sample, control, blank, etc.) of
each well on the plate, including the number of
replicates, orientation, and fill direction.
Blank Mode Select a pre-defined blank mode or define a
custom blank mode.

Software
Option (Cont’d) Purpose
Data Reduction Menu:

QC Raw Data Define a quality control equation to be applied
to the raw data obtained when running the
assay.
Threshold Define threshold equations, positive/negative
Q.C. ranges, threshold Q.C. equations, and the
results output format.
Curve Fit Specify the parameters required to obtain
quantitative results with a user-defined curve fit.
Ratio Define the equation and output format required
to report results as a ratio.
Spreadsheet Perform arithmetic calculations on ODs from
different wells. The results of these calculations
are output as a matrix or table of data.
Report Options Select report formats, matrix type, and export
files.
Assay Options Specify area statistics, processing order, and
sample ID setup.

Software
Worklist Creation
A worklist specifies the samples that are to be run, the Sample ID
corresponding to each sample tube, and the assay(s) to be run on each
sample. A worklist can include up to four microplates and multiple assays.
More than one assay can be run on a plate if the assays have the same
incubation, washing and shaking specifications.
Note: An assay must be created before it can be assigned to

samples on a worklist.
The procedure for creating a worklist is summarized on page 59.
Worklist Execution
Once a worklist has been created, the run can be started. The system
prompts the operator to load any microplates and consumables that are
required by the worklist.
Note: The procedure for running a worklist is summarized on

page 65.
Data Analysis
The optical density results for each well on each of the microplates in the
worklist are analyzed according to the criteria that were specified during
assay definition.
Results Reporting
When the processing of a microplate is completed, the results of the run are
displayed on the screen.
Results files are automatically named xxx.dat, where xxx is the name you
selected for the plate. Results are stored in the Plate Data directory.
Note: Select the Options command on the Tools menu to view or

change a directory in which results files or other files are located.
Data Backup
Revelation™ software allows the user to save all assays and worklists on
floppy diskettes to avoid loss of data.

Software
Required But Not Provided
Computer
A personal computer with monitor, keyboard, mouse and printer is required
for operation of the DSX™ Automated ELISA System.
The computer system provides the means for you to enter information and
obtain results. The computer stores and retrieves assay profiles, executes
the defined worklist, and performs the various calculations needed for the
assay.
The computer system that is used for operation of the DSX™ Automated
ELISA System must meet the following minimum requirements:
• Pentium microprocessor running at 500 MHz or better.
• Hard disk with at least 100 MB of free space.
• Microsoft® Windows® NT operating system.
• VGA graphics card (Super VGA recommended). Monochrome,
CGA, EGA or calibrated monitors are not supported.
• The Display properties should be set to a desktop area of at least
600 x 800 pixels and a color palette of at least 256 colors.
• At least 64 megabytes (MB) of random-access memory (RAM).
(128 MB RAM is recommended).
• One unused RS232 serial port is required for connecting the
computer to the DSX™ Automated ELISA System.
• Mouse or other pointing device supported by Windows.
• Any printer that is supported by Windows® NT can be used.
• Compatible sound card

Specifications
Specifications
Dimensions and Weight

Depth <91 cm (35.8 in)
Width <106 cm (41.8)
Height <80 cm (31.5 in)
Weight <110 Kg (243 lbs)
Footprint 106 cm x 91 cm (41.8 in x 35.8 in)
Capacities
Samples 96 samples (98 positions are available)
Reagents 24
Controls and/or Standards 33
Sample Pipetting Tips 432
Reagent Pipetting Tips 41
Wash Buffer Bottles 4 bottles, 2 liters each
Waste Container 8 liters
Operation
Ambient Drawer Module Ambient plus 5 °C
Incubation Temperatures
Incubator Module Ambient plus 7 °C to 50 °C

Incubation Temperatures
Assays
Blanking Air
Individual, paired or average wells
Whole plate or last plate
Row or column
Each well on the plate
Wavelength Modes Single, dual or multiple
Standard Curves Linear, quadratic, cubic, quartic, spline, polygon,

sigmoid or Akima
Additional Data Analysis Threshold, ratio, QC equations
Flexible Template Up to eight different well types.

Specifications
Power Requirements
Voltage Power Frequency
Main Unit 100 - 240 V 800 VA 50/60 Hz
Line Voltage Variation ± 10%
Line Frequency Variation ± 3 Hz
Environmental
Operating Range 15° C to 30° C
15% to 85% relative humidity (non-condensing)
2000 Meters Altitude
Computer Interface
Ports RS232 serial port
Baud Rate 19200. Character format.
Character Format 7 data bits, 1 stop bit, no parity
Standards
The instrument is designed in accordance with CSA 1010-1, CSA 1010-2-010,
UL 3101-1, EN 61010-1, EN 61010-2-010 and EN 61326-1.

Warning Labels
Warning Labels
The DSX™ Automated ELISA System or its components may contain
certain labels that that either warn the user of a hazard or note an electrical
connection. A hazard is something that can cause personal injury to the
operator or damage to the instrument. The labels that may be used on the
DSX™ Automated ELISA System are described below.
Label Description
Alternating current is present.
(English) Caution symbol. Refer to the

Routine Maintenance chapter.
(French) Attention (voir documents
d’accompagnement).
(English) Caution, motion hazard.

(French) Attention
(English) Caution, pinching or mechanical hazard.

(French) Attention
(English) Caution, hot surface.

(French) Attention, surface chaude.
Laser radiation – Do not stare into beam.
Protective conductor terminal.
Earth (ground) terminal.
(English) Caution, risk of electric shock.

(French) Attention, risque de choc électrique.
Caution, biohazard.

Unpacking
Chapter 3 Installation
IMPORTANT: These installation procedures are intended for

trained personnel
Unpacking
Materials Provided
Article Quantity
DSX™ Automated ELISA System 1
Instrument Power Cable 1
RS232 Communication Cable 1
Cleaning Wires (Aspirate and Dispense) 2 Each
CD Containing Revelation™ Software Setup 1
Program and Electronic Operator’s Manual
Shipping Check List 1
Wash Buffer Containers 4

Liquid Waste Container 1
Reagent Rack and Tip Funnel 1 Each
Tip Waste Container 1
Computer and Monitor (required, but sold 1
separately))
Consumables Sample Kit 1
4 mm Allen Wrench 1
Sample Caddy 1
Sample Racks 7
Printer (Parallel) Cable 1
Alignment Fixture 1
Purge Trays 5
Deep Well Base 2
Control Rack 1
Plate Holders 4
8-way Wash Head 1
Washer Plate Holder 1

Positioning the Instrument
To unpack the components:
1. Unpack the contents of the carton.
CAUTION: The contents are heavy (approximately 110 Kg

or 240 lbs).
2. Place the DSX™ Automated ELISA instrument in the

approximate position where it will be located.
3. Examine the packaging to be sure that the power cord, the
computer connector cord and other materials have been
removed. Please save packaging material for future use.
4. Check to verify that all of the materials listed on the previous
page have been unpacked.
5. Inspect the components for damage. If damage is observed,
contact your shipper or service representative immediately.
Positioning the Instrument

Determine the area where the system will be located. You will need an area
that is approximately 106 cm (41.8 inches) wide, 91 cm (35.8 inches) deep,
and 80 cm (31.5 inches) high for the DSX™ Automated ELISA System.
The system should be positioned on a level surface that does not support
other devices that produce vibration (centrifuges, shaker bath, etc.). There
must be at least 20 cm (7.9 inches) of space at the rear of the instrument to
allow for sufficient ventilation.

Connecting the Computer System
Connecting the Computer System
Note: The computer system is required but not provided.
To connect the computer system:
1. Place the computer, keyboard, monitor and printer next to the

DSX System.
2. Plug the RS232 communication cable into an unused RS232
port on the computer. Note the computer port (i.e., COM1 or
COM2) that is used.
Note: Refer to the instructions accompanying the

computer for the location of the ports and for
information on connecting components.
3. Plug the other end of the RS232 communication cable into the
bottom RS232 port at the right side of the instrument (Figure 19).
Note: The lowest of the 3 RS232 ports must be used.

The other RS232 ports are used for diagnostic
purposes. See Figure 19.
4. Connect the keyboard, monitor and printer to the computer.

5. Connect the power to the computer, monitor and printer.
Cooling Power Power Cord

Fan Switch Connector
RS232 Ports
for Diagnostic
Purposes
RS232 Port for

Connection
to Computer
Figure 19. View of the Right Side of the DSX™ Automated ELISA System

Loading Revelation™ Software

Revelation™ software is provided on a CD ROM. Before installing the
software on the personal computer, you will need the following information:
• The installation drive. Usually, this will be c:.
• The installation directory.
To install Revelation Software:
1. Start Microsoft Windows NT.

2. Insert the installation CD ROM. The installation program will start
automatically and the Revelation™ Installation Window will be
displayed.
Note: If the installation program does not start

automatically, select Start and then Run from the task
bar. In the Run text box, browse to setup.exe on the
CD ROM drive, click Open, and then click OK.
Note: To stop the installation, click on Exit.
3. The prompt Setup is complete is displayed when the installation

has been completed. Click on OK and remove the installation
CD ROM.
4. The Revelation™ program group is accessed from the Programs
section of the Start task bar.
Note: The Revelation™ program icon can be added to

your Windows NT desktop using the Settings section
of the Start task bar.
Or, a Revelation™ shortcut icon can be placed on your
computer desktop for convenient access to
Revelation™. See the following section for instructions.

Creating a Shortcut Icon

A Revelation™ shortcut icon can be placed on your computer desktop in
two ways:
• (Recommended) A shortcut program is created during installation.
This program can be dragged to the desktop from Windows
Explorer.
• A new shortcut can be created.
To use the installed shortcut:
1. Open Microsoft Windows Explorer.

2. Locate the Revelation™ shortcut in the directory
c:\Windows\Start Menu\Programs\Revelation.
3. Drag the Revelation™ shortcut icon to the desktop.

Note: Be sure to drag the Revelation™ shortcut, not
the Revelation™ Help shortcut.
Alternately, right click Revel.exe in the same directory and select

Desktop as Shortcut from the Send To popup menu.
4. Close Microsoft Windows Explorer.

Connecting the DSX Power Cord
Connecting the DSX Power Cord

The power cord connection to the DSX is made at the right side of the
system.
Note: Depending upon local electrical codes and electrical service
quality, an optional uninterruptible power supply (UPS) may be
required in your laboratory. The use of a UPS is optional but
recommended.
CAUTION: The DSX System must be connected to

properly grounded electrical outlets. Obtain assistance from
a qualified electrician to verify that your electrical outlets are
properly grounded.
Before connecting the power cable, be sure that the

components have been connected to each other as outlined
in the previous section.
To connect the power cord:
1. Connect the power cord to the connector at the right side of the
instrument (Figure 19).
2. Connect the other end of the power cord to the laboratory
electrical supply outlet.

Starting the System
Starting the System

To start the system:
CAUTION: Before starting the system, be sure that all

racks are properly seated and that the lids are removed
from all tubes, plates and sample tip racks.
1. Turn the DSX System power switch ON (Figure 19).

2. Turn the power ON (if necessary) for the computer, monitor and
printer.
3. Double-click the Revelation™ shortcut icon. Or, select
Revelation from the Program menu in Windows NT.
4. Type Thermo as the default system password (this can later be

changed by selecting System Password from the Tools menu).
5. Click OK. Startup choices are displayed (Figure 20).
6. Select Connect to DSX to operate the DSX System. Or, select

Configure Hardware to display the Setup DSX dialog box for
configuring the system (see page 45).
Note: Select Connect in Demo Mode to run the
sofware in demonstration mode. The DSX System is
not connected during demonstration mode. Select Quit
Revelation to exit from the Startup dialog box.
7. Select Do It. A series of self-tests is performed (see the following

page).
Figure 20. Startup Dialog Box

Self Tests
Self Tests
A series of self-tests are carried out after Connect to DSX is selected from
the Startup dialog box. The test results are displayed upon completion of
the tests (Figure 21).
The self-test results should be printed on a monthly basis so that a record
can be kept of the performance of the DSX System. You can save test
results (the test results files are named *.tst) and then open them for review
or printout at a later date.
Note: The self-test results should indicate whether the system is
operating properly. If a self-test failure is reported in the Self-Test
window, it is recommended that you call Technical Service.
To print self-test results:
1. Select Print from the File menu while the self test results are
displayed.
Note: If the DSX data files have been corrupted, the
DSX prompts for the serial number. This can be found
on the DSX rear panel.
Figure 21. Display of Self-Test Results

Configuring the DSX
Chapter 4 Preparing the System for Use
Configuring the DSX

The DSX can be configured for particular uses in your laboratory. Refer to
page 28 for a description of configuration options that are available.
To configure the DSX:
1. Select Configure System from the Tools menu. The Setup DSX
dialog box is displayed (Figure 22).
Note: The Setup DSX dialog box can also be displayed
by selecting Configure Hardware from the Startup
dialog box (Figure 20).
2. Specify the settings for each of the configuration options.

Note: Detailed instructions for an operation are
contained in the DSX Online Operator’s Manual,
accessed by selecting the Help button on the dialog
box that is being used.
Figure 22. Setup DSX Dialog Box

Editing Washer Plate Settings

The wash head on the DSX must be aligned so that the tips of the wash
pins are aligned with the base of the washer when the wash module is in
Standby.
To edit the washer plate settings:
1. Obtain a microplate of the type for which the washer is to be

aligned.
2. Eject the washer plate carrier and carefully place the microplate
in the carrier.
3. Retract the plate carrier.
4. Select Edit Washer Plates… from the Tools menu. The Edit
Washer Plate Settings dialog box is displayed (Figure 23).
5. Click New and enter a name for the type of plate for which the
wash settings are being entered.
Or: If settings for a plate that is already defined are being edited,
select the plate from the Plates Currently Defined panel.
6. Click the Show button opposite the Dispense Height field to

position the wash head in the dispense height position.
Note: Refer to page 17 for a description of the various
wash height positions.
7. If the dispense height needs to be changed, enter the new value

in the Dispense Height field.
8. Click the Show button opposite the Dispense Height field to

position the wash head in the new dispense height position.
9. Repeat Steps 7 and 8 until the dispense height is correct.

Figure 23. Edit Washer Plate Settings Dialog Box
10. Repeat Steps 6 through 9 for each of the other wash height
positions.
11. Select the sweep mode and the sweep stroke.

Important: Select No Sweep for sweep mode and
disable bottom washing whenever a C-bottom,
U-bottom or V-bottom plate is being used.
12. If sweep mode is enabled, specify the sweep stroke.

13. Click on Close to save the entered values.
Note: Detailed instructions are contained in the DSX
Online Operator’s Manual, accessed by selecting the
Help button on the dialog box that is being used.

Selecting the Results Font
Selecting the Results Font

The user can select the font that is used when results reports are printed.
To select the results font:
1. Select Results Font from the Tools menu. The Font dialog box
is displayed (Figure 24).
2. Select the results font.

Figure 24. Font Dialog Box

Setting Options
Setting Options
Options allow the user to create assay short-cut buttons, set default plate
processing options (including auto save and auto print), select colors for
different well types, set default directories and enter the laboratory name,
address and phone number(s) to be printed on results reports.
To set options:
1. Select Options from the Tools menu. The Options dialog box is
displayed (Figure 25).
2. Specify the settings for each of the Options tabs.

Figure 25. Options Dialog Box

Changing the Password
Changing the Password

The system password can be changed.
To change the system password:
1. Select System Password from the Tools menu. The System

Password dialog box is displayed (Figure 27).
2. Specify a new system password.

Figure 26. System Password Dialog Box

Specifying Levey Jennings Criteria
Specifying Levey Jennings Criteria

Levey-Jennings statistical charts allow the user to select a specific control
from within a specific Assay template and view variation over time, in a
graphical format. Levey-Jennings charts can be displayed against raw O.D.
values or against calculated results (concentrations or ratios). Westgard
rules may also be applied. Levey Jennings criteria are specified during the
Levey Jennings analysis.
To specify Levey Jennings criteria:
1. Select Levey Jennings from the Tools menu. A dialog box is

displayed for selections of an assay.
2. Select the assay for which a Levey Jennings verification will be
performed. A dialog box is displayed for entry of the well type.
3. Select the well type and click OK. A new screen is displayed for
selection of Levey Jennings criteria (Figure 27).
4. Specify the Levey Jennings criteria.
Figure 27. Screen for Entry of Levey Jennings Criteria

Specifying Consumables
Specifying Consumables
A database of all consumables and fluids is maintained in the software.
Once a consumable or fluid is defined in the database, it is available for
selection from a drop-down list during definition of an assay.
To enter information for a consumable:
1. Select the appropriate command (i.e., Edit Washer Fluids…)

from the Tools menu. The corresponding dialog box is
displayed.
2. Reagent bottles, Sample tips and Reagent tips are all pre-
defined in the Revelation™ software. Refer to Reagents and
Pipetting in Chapter 1 Overview for the ordering information.

Filling the Wash Buffer Containers
Filling the Wash Buffer Containers

The Wash Buffer Containers must be filled with the appropriate wash buffer
and the dispense tubing and dispense pump power cable must be
connected to the front of the instrument.
To fill the Wash Buffer Containers:
1. Disconnect a Wash Buffer Container (see page 19) and remove

it from the system.
2. Remove the rear cover of the Wash Buffer Container
(Figure 13) and fill it with the wash solution that is to be used.
Note: The Wash Buffer Containers each contain up to
two liters. DO NOT OVERFILL.
3. Make sure that the dispense tubing is routed through the

appropriate pinch valve at the front of the instrument.
4. Connect the dispense tubing to the Wash Buffer Container and
connect the Wash Buffer cable to the connector at the front of
the instrument (refer to Figure 13 for details).
Note: Be sure that the wash tubing is not pinched or
crimped.
5. Repeat Steps 1 through 4 for the remaining wash buffer

containers.

Connecting the Waste Container
Connecting the Waste Container

The Waste Container must be connected to the front of the instrument.
To connect the Waste Container:
1. Route the waste tubing through the routing holes inside the front
panel of the instrument.
2. Connect the vacuum tubing and the waste tubing to the
connectors at the front of the instrument.
Note: The connectors for the vacuum tubing and the
waste tubing are a different size so they cannot be
reversed.
Be sure that the waste tubing and the vacuum tubing
are not pinched or crimped.
3. Connect the level sensor cable to the connector at the front of

the instrument.

Barcode Label Suggestions

The following are suggested for optimizing the success of barcode label
reading on sample tubes and plates:
1. When using an Ink Jet label maker, large dot spacing may
increase the number of blank scans. Therefore, test the labels
before using them in an assay. The print quality is very important
to reliable scans.
2. Ensure the barcode density selected is between 5.0 mil and 13.0
mil.
3. The Vertical Scanner can read test tube barcodes up to 85mm in

length, including the 5 mm Quiet Zone on each side of the
barcode. However, this may vary, depending upon the code type
and barcode density. The operator should test to see if a specific
barcode topology can be reliably scanned.
4. The Horizontal Scanner can read barcodes that are between

26mm and 50mm in length.
5. Check that the Quiet Zone is sufficiently wide at both ends of the
barcode label. This distance must be at least 5 mm from the
actual bar code to the end of the label. Be sure that the Quiet
Zone is not reduced at the corner of a plate, otherwise a blank
scan could occur.
6. The test tube barcode that is to be scanned must be properly

aligned so that the barcode label faces out of the sample rack
slot.
7. For plate scanning, ensure that the selected scan area (left,
right, or center) is consistent with the placement of the barcode
labels on the plates.
8. Place the code side of the barcode label as close to the top of
the plate as possible. This requirement is dependent upon the
code type and barcode density, and may require trimming the
top of the label.
9. Check that the digits, letters, and other characters are correct for
the barcode symbology that is being used. Also, use the correct
number of data digits for a specific barcode symbology.

10. The following symbologies are supported: Codabar, Code 39,

Code 93, Code 128, Standard 2 of 5, Interleaved 2 of 5,
MSI/Plessey, and UPC / EAN / JAN. However, symbologies with
poor internal verification or partial decodes (such as Interleaved
2 of 5, Standard 2 of 5, and MSI/Plessey) should be avoided if
possible.
11. In Revelation, enable only the barcode symbology (or

symbologies) that will be used.
12. Be sure that the barcode scanner module windows are clear,
clean, and are not scratched.

Creating a New Assay
Chapter 5 Defining an Assay
Creating a New Assay

The assay(s) for a worklist must be created before a worklist can be
prepared. Assay operations (pipetting, dispensing, washing and
incubating) and data reduction steps (see page 30) are specified in the
assay.
To create a new assay:
1. Select New then Assay from the File menu. A default assay is
displayed.
2. Define the assay operations and data reduction steps as
outlined below.
To define assay operations:
1. Display the default assay (see above).

2. Select the desired assay operation from the Operations menu.
The appropriate dialog box is displayed.
3. Define the operation.
To define data reduction steps:
1. Display the default assay (see above).
2. Select the desired data reduction step from the Data Reduction
menu. The appropriate dialog box is displayed.
3. Define the data reduction step.

Modifying an Assay
Modifying an Assay
An assay can be modified at any time.
To modify an assay:
1. Select Open from the File menu. The Open dialog box is
2. Select the assay to be modified.
3. Modify the assay operations and/or data reduction steps as
outlined on the previous page.
Figure 28. Open Dialog Box

Creating a Worklist
Chapter 6 Creating a Worklist
Creating a Worklist
A worklist must be created before assays can be run on samples. The
worklist specifies the samples that are to be run, their location on the
sample caddy, and the assay(s) to be run on each sample.
Note: A worklist can be created after at least one assay has been
created. The worklist can include assays run on up to four different
microplates.
More than one assay (up to 12) can be run on one plate if the
assays have the same incubation, washing and shaking
specifications.
Only one worklist can be open at a time.
To create a new worklist when Sample IDs are scanned:
1. Select New then Worklist from the File menu. The Sample
Batch Selection dialog box (Figure 29) is displayed.
2. Select Add assays using a new batch of samples.
Note: If additional samples are being added to an

existing worklist or if the worklist is being otherwise
modified, select Add assays to an existing sample
batch and follow the instructions on page 63.
3. Place a check mark next to Scan barcodes on new sample

batch. If data for the new batch is to be sent to the LIMS, place
a check mark next to Use LIMS on new sample batch.
Figure 29. Sample Batch Selection Dialog Box

Creating a Worklist
4. Click OK. The Sample ID Barcode Scanning dialog box is

Note: The position of each sample tube on the sample

caddy is shown in the Sample ID Barcode Scanning
dialog box.
5. Click on the symbol for the last tube and then select Last
Sample from the popup menu. The symbols for each of the
tubes to be run are displayed in white.
6. Click Load Sample Caddy. The Load Sample Rack dialog box
is displayed (Figure 31).
7. Place the filled sample rack(s) on the sample caddy, observing
the instructions displayed on the Load Sample Rack dialog box.
8. Click the check mark on the Load Sample Rack dialog box. A
message box is displayed.
9. Follow the instructions on the message box.
10. Click Scan Sample IDs. The DSX will scan the barcode labels of
the samples designated in Step 5. When completed, the Edit
Worklist dialog box is displayed with the Sample IDs entered into
the corresponding sample rack positions (Figure 32).
11. Click Add Assay to display a dialog box for selecting an assay.
Select the assay and click OK.
Figure 30. Sample ID Barcode Scanning Dialog Box

Creating a Worklist
12. In the column under the assay, click in the row for a sample to
order that assay for the sample.
13. Repeat Steps 11 and 12 to order additional assays.
14. Click OK to save the worklist.
Note: Click Cancel before clicking OK to cancel the

worklist. Once the worklist is saved, samples cannot be
added to a worklist for scanned Sample IDs.
Figure 31. Load Sample Rack Dialog Box
Figure 32. Edit Worklist Dialog Box (Scanned Sample IDs)

Creating a Worklist
To create a new worklist when Sample IDs are manually

entered:
2. Select Add assays using a new batch of samples.
Note: If additional samples are being added to an

existing worklist or if the worklist is being otherwise
modified, select Add assays to an existing sample
batch and follow the instructions on page 63.
3. If data for the new batch is to be sent to the LIMS, place a check
mark next to Use LIMS on new sample batch (Figure 29).
4. Click OK. The Edit Worklist dialog box is displayed (Figure 32).
5. Click Add Assay to display a dialog box for selecting an assay.

Select the assay and click OK.
6. In the column under the assay, click in the row for a sample
position to order that assay for the sample.
7. Repeat Steps 5 and 6 to order additional assays.

8. Type each Sample ID into the box adjacent to the corresponding
sample position.
Alternately, enter the starting Sample ID in the First Sample ID
box and then click Assign Sample IDs to automatically assign
sequential Sample IDs.

Modifying a Worklist
A worklist must be created before assays can be run on samples. The
worklist specifies the samples that are to be run, their location on the
sample caddy,
To modify a worklist:
2. Select Add assays to an existing sample batch.

3. From the drop-down list, select the worklist that is to be modified.
Note: Click View Samples to display the samples in

the currently selected worklist.
4. Click OK. The Edit Worklist dialog box is displayed (Figure 32)
containing information for the selected worklist.
5. Modify the worklist as needed.
Note: Samples cannot be added to a worklist for

scanned Sample IDs.


The Run Timeline Screen
Chapter 7 Running a Worklist

Prepare for a run by opening the worklist and displaying the run timeline
screen. From this screen, you can examine details of the worklist, examine
a diagram of the plate layout, and review the run settings.
To display the run timeline screen:
1. Click on the Show Timeline tool at the top of the screen. The
run timeline screen is displayed (Figure 33).
Show Timeline
tool Note: Click on the Hide Timeline tool to cancel display
of the run timeline screen.
Hide Timeline
tool Note: The toolbar contains tools for starting and
stopping the run, changing the display, and setting run
options.
Right-click the mouse anywhere to display a popup

menu of additional run settings.
Key to
Toolbar Symbols Timeline
Figure 33. Run Timeline Screen

To view the worklist or plate layout diagram:
1. Select Worklist or Plate Layout from the drop-down list at the

top of the screen.
Note: When Plate Layout is selected, the assay can

be selected from a second drop-down menu.
Timeline Screen Toolbar
The timeline toolbar has tool icons for performing common run functions:
Tool Description
Show Timeline. Displays the run timeline screen.

Diagrams of the run timeline, the worklist, and the plate
layout can be selected from this screen.
Hide Timeline. Cancels display of the timeline screen.
Start Run. Begin assays on the samples.
Stop Run. Cancel the run.
Pause Run. Temporarily halt the run.
Fast Forward. Advance the run to a later portion.
Zoom In. Increase magnification of the timeline.
Zoom Out. Decrease magnification of the timeline.
e-Mail. Contact the designated individual(s) by e-mail if

the run needs attention.
Sound. Sound a tone if the run needs attention.

Right Mouse Menu
A menu of additional run commands and settings (Figure 34) is displayed by

right-clicking the mouse anywhere on the run timeline screen.
Figure 34. Right Mouse Menu
Command Description
Timeline Displays a submenu of commands:

Execution Start. Start the run.
Stop. Stop the run.
Pause. Temporarily halt the run.
Fast Forward. Advance the run to a later portion.
View Displays a submenu of commands:

Zoom In. Increase magnification of the timeline.
Zoom Out. Decrease magnification of the timeline.
Contact Displays alternatives for creating an alert if the run

Options needs attention:
Contact Me By E-Mail.
Contact Me By Playing a Sound.
Settings Displays a submenu of commands:

Runtime Options. Displays the Runtime Options
dialog box (page 68).
Error Recovery. Displays the Error Recovery Options
Edit Symbols. Displays the Edit Timeline Symbols
Edit Contact Details. Displays the Edit Contact details
Log Displays a dialog box for entry of a free text note that
Operator will be appended to data for the run.
Message

Reviewing Run Settings
To review runtime options:
1. Display the run timeline screen.
2. Select Settings then Runtime Options from the right mouse

popup menu. The Runtime Options dialog box (Figure 34) is
displayed.
3. If needed, modify the runtime options. Detailed instructions are
contained in the DSX Online Operator’s Manual, accessed by
selecting Online Manual from the Help menu.
Figure 35. Runtime Options Dialog Box

To review error recovery options:
2. Select Settings then Error Recovery from the right mouse

popup menu. The Error Recovery Options dialog box (Figure 36)
is displayed.
3. If needed, modify the error recovery options. Detailed
instructions are contained in the DSX Online Operator’s Manual,
accessed by selecting Online Manual from the Help menu.
Figure 36. Error Recovery Options Dialog Box

To review timeline symbols:
2. Select Settings then Edit Symbols from the right mouse popup
menu. The Edit Timeline Symbols dialog box (Figure 37) is
displayed.
3. If needed, edit the symbols. Detailed instructions are contained
in the DSX Online Operator’s Manual, accessed by selecting
Online Manual from the Help menu.
Figure 37. Edit Timeline Symbols Dialog Box

To edit contact details:
2. Select Settings then Edit Contact Details from the right mouse
popup menu. The Edit Contact Details dialog box (Figure 37) is
displayed.
3. If needed, edit the contact details. Detailed instructions are
contained in the DSX Online Operator’s Manual, accessed by
selecting Online Manual from the Help menu.
Figure 38. Edit Contact Details Dialog Box

Checking Consumables and Fluids
Checking Consumables and Fluids

Before starting the run, empty the waste container and fill wash buffer
bottles with wash buffer.
To empty the Waste Container:
1. Disconnect the Waste Container (see page 21) and remove it

from the system.
2. Remove the cover of the Waste Container and discard the waste
in accordance with local regulations.
3. Rinse the waste container with DEIONIZED water. Discard the
rinse water.
Note: If desired, the waste container can be disinfected

with a 10% (v/v) solution of household bleach in water,
or 70% ethanol. If bleach is used, the container must
be thoroughly rinsed with deionized water before
replacing, as residual bleach may affect the results of
ELISA assays.
4. Replace and tighten the waste cap.
Note: Be sure that the waste cap is securely tightened.

Otherwise, a vacuum leak will cause the software to
create a vacuum error condition.
5. Place the Waste Container on the system and connect the

fittings.
To fill a wash buffer bottle:
1. Disconnect the Wash Buffer Container (see page 19) and

remove it from the system.
2. Remove the rear cover of the Wash Buffer Container
(Figure 13). Lay the cover on a clean paper towel.
3. Fill the wash buffer bottle with the wash buffer that is to be used.
DO NOT OVERFILL.
Note: If the wash buffer in a bottle is being changed,

discard the contents of the bottle and thoroughly clean
it before filling it with the new wash buffer.
4. Replace the cover and tighten it.

5. Place the Wash Buffer Container on the system and re-connect
the fittings.

Starting the Run
Starting the Run

To start the run:
1. Display the worklist to be run (if needed).
2. Select the Start icon. A dialog box to load the first item needed
for the run is displayed (Figure 39).
Start
icon
Note: The prompts that are displayed depend upon the
worklist. In the example shown, the samples have not
yet been loaded on the system. If Sample IDs are read
automatically, the samples will already be on the
system and this prompt will not be displayed.
3. Load the item and click the check mark to display the next
prompt.
4. The Load Plate dialog box (Figure 40) is displayed when the
microplate(s) for the run are to be loaded. Place the first
microplate on the plate drawer in the position shown.
5. Enter the microplate ID and click the check mark to display the
next prompt.
Note: If the microplate barcode label is to be read,

designate the position of the barcode label on the
microplate and click Read Barcode instead of entering
the microplate ID.
Figure 39. Load Sample Rack Dialog Box

Starting the Run
6. Follow additional instructions, making sure to load each item at

the designated position. After an item is loaded and information
(if any) is entered, click the check mark to display the next
prompt.
Important: All racks are mounted on dowel pins. Be

sure that each rack is securely seated and cannot
move in either the x- or y- direction.
7. Detailed instructions are contained in the DSX Online Operator’s

Manual, accessed by selecting Online Manual from the Help
menu.
Figure 40. Load Plate Dialog Box

Monitoring Run Status
Monitoring Run Status
To monitor status of the run:
1. Display the worklist (if needed).
2. Select Worklist, Timeline, or Plate Layout from the Timeline

screen. The corresponding view of the run is displayed
(Figure 41).
3. Detailed instructions are contained in the DSX Online Operator’s
Manual, accessed by selecting Online Manual from the Help
menu.
Figure 41. Timeline View

Modifying the Worklist During a Run
Modifying the Worklist During a Run

Samples and/or assays can be added to the worklist during a run.
Note: Samples cannot be added to a worklist if Sample IDs are

read by the barcode reader.
To modify the worklist during a run:
2. Select Add assays to an existing sample batch.

3. From the drop-down list, select the worklist that is to be modified.
Note: Click View Samples to display the samples in

the currently selected worklist.
4. Click OK. The Edit Worklist dialog box is displayed (Figure 32)
containing information for the selected worklist.
5. Modify the worklist as needed.
Note: Samples cannot be added to a worklist for

scanned Sample IDs.

Safety Suggestions
Chapter 8 Safety
Safety Suggestions
The following information should assist in the safe and efficient use of the
DSX. It is important to read, understand and follow the considerations listed
in this chapter. In addition to the warning labels and other cautions
previously described in this manual, consider also the following:
1. The removable workspace components must be seated properly

on the workspace pins. This is necessary for the component
coordinates to be properly aligned.
2. Sample tubes must be pushed down in the sample racks to
prevent obstructing the pipette module.
3. Sample racks must be properly placed into the sample caddy,
with the tube opening facing the washer module (and scanner
module). Seat the “V” on the rack into the notch on the caddy.
Racks should be level and flush.
4. Different rack configurations exist to accommodate sample tubes
of varying heights. Be sure to use the proper rack for the sample
tubes being used.
5. Periodically inspect the sample rack springs to better assure
proper tube alignment. Replace as necessary.
6. The new sample caddy must be used for sample tube bar code
scanning. This accepts the new pick-up type racks for scanning
of tube bar codes. There are a few ways to tell the two apart:
7. There is a small gap between the racks, when seated in the new
caddy. The new caddy has handle holes on the side and a full
base. The old caddy has solid sides and a “see through” base.
8. Appropriate precautions must be taken when working with
biohazards or potential biohazards. Training in the safe handling
and clean-up of potential blood borne pathogens should be
performed. Universal precautions, appropriate hygiene and
decontamination of surfaces are recommended.
9. Bar code labels should be applied with a straight orientation onto
the sample tubes. Do not rotate the label away from the opening
in the sample rack.
10. Bar code quality can be critical to successful scanning of sample
tubes. Scanning is in accordance with ASTM E1466-92 defining
barcode quality, position and orientation of barcode labels.

Safety Suggestions
11. The DSX scanner module is capable of reading many different

bar code symbologies (IATA, WPC, Code 39, Codabar, 2 of 5,
Code 93, Code 128, MSI/Plessy, UPC-A, UPC-E, EAN-8, EAN-
13). The use of non-standard bar codes or bar codes with poor
print quality may be problematic.
12. Attend to error messages when the system prompts and stops.
These messages indicate the need for user judgement and
action. Error messages can be also be delivered with an audible
alarm (sound card in PC is needed), or sent via e-mail.
13. As with any computer application, periodic back-up of electronic
files is recommended.
14. Changes to assay files may impact the suitability and plotting of
data using the Levey-Jennings control charting feature.

Routine Maintenance Procedures
Chapter 9 Service and Maintenance
The following periodic maintenance procedures are required for the

DSX™ Automated ELISA System:
Daily maintenance:
• Verify that the self-test passes.
Note: Results of the self-test can be printed if desired.

Refer to page 44 for specifying printing of self-test
results.
• Empty and clean the Waste Tip Container.
Warning: While the DSX alone does not present a

biohazard, the samples that are used and all parts and
consumables in contact with the samples must be
considered biohazardous.
• (As needed) Empty and clean the Liquid Waste Container.
Note: If desired, the Waste Tip Container and the

Liquid Waste Container can be disinfected with a 10%
(v/v) solution of household bleach in water, or 70%
ethanol. If bleach is used, the containers must be
thoroughly rinsed with deionized water before replacing,
as residual bleach fumes may affect the results of
ELISA assays.
• Clean all plate drawers and external surfaces, using a towel

moistened with 70% alcohol.
• Purge the washer with 50mL of deionized water.
Note: The deionized water used for purging should be

placed in Wash Buffer Container D (see Figure 12).

Weekly maintenance:
• Empty the Wash Buffer Containers and clean them with several
rinses of deionized water.
• Remove and clean the waste tip chute.
Note: If desired, the waste tip chute can be disinfected

with 70% alcohol or by autoclaving it. Bleach should not
be used since it will corrode the metal.
Six month maintenance:

• Replace the dispense tubing.
• Replace the aspiration tubing.
Note: The dispense tubing and aspiration tubing may

need to be replaced more frequently than every six
months, depending upon the frequency of use and the
severity of operating conditions.
Contact THERMO LABSYSTEMS for information on
replacement tubing.

Cleaning and Decontamination
Cleaning and Decontamination
The DSX™ Automated ELISA System is constructed from materials that

resist chemical attack.
Spills should be cleaned as soon as possible. If you need to decontaminate
the DSX™ Automated ELISA System instrument (for example, before
servicing the instrument), clean the system and then decontaminate it as
described below.
CAUTION: Always disconnect the power cable before

cleaning the instrument.
To clean the system:
1. Clean external surfaces with a cloth moistened with mild

laboratory detergent.
Note: If needed, dilute the laboratory detergent

according to the manufacturer’s instructions before
using.
To decontaminate the system:
1. Wipe the surfaces with a cloth moistened with a 10% (v/v)

solution of household bleach in water or a 70% (v/v) solution of
alcohol.
Note: Remove residual bleach from surfaces with a

cloth moistened with deionized water. Residual bleach
may affect the results of ELISA assays.

Removing a Module
Removing a Module
The incubator modules, absorbance module and the wash module can be
easily removed from the DSX™ Automated ELISA System.
To remove an incubator module or the absorbance module:
1. Loosen the fasteners (Figure 42) one-fourth (¼) of a turn using a

4 mm Allen wrench.
Note: The incubator modules and the absorbance

module each have two fasteners.
2. Pull the module out of the housing.

3. Replace the module by reversing the procedure.
Fasteners
Figure 42. Fasteners on the Incubator Modules or Detection Module
To remove the wash module:
1. Remove the wash head by loosening the thumb screw.

2. Disconnect the tubing from the dispense valve on the washer
module.
3. Loosen the fasteners one-fourth (¼) of a turn using a 4 mm Allen
wrench.
Note: The wash module has four fasteners.
4. Pull the module out of the housing.

5. Replace the module by reversing the procedure.

Replacing the Absorbance Module Lamp
CAUTION: The optics assembly may be hot. Allow at least

five minutes for the instrument to cool before opening the
optics assembly.
Also, be careful when removing the filter access panel as

there is a possibility that the bulb may have broken.
Failure to follow the lamp replacement procedures as

described may result in personal injury.
To replace a lamp:
1. Remove the absorbance module from the system.

2. Remove the filter access panel to expose the optical assembly.
The bulb is mounted in the upper portion of the optical assembly
as shown in Figure 43.
Lamp
Figure 43. Optical Assembly

3. Put on a pair of rubber or latex gloves.
CAUTION: Gloves are necessary to prevent skin oils from

damaging the lamp. Gloves are also worn as a safety
precaution should the glass bulb accidentally break.
4. Grasp the lamp with plastic tweezers and pull the lamp out of its
receptacle (Figure 44).
IMPORTANT: Use a gloved finger to prevent the bulb

from bumping into the metal sidewalls of the enclosure
during removal.
5. Obtain a replacement lamp.

6. Grasp the lamp tines with plastic tweezers and align the tines
with the corresponding holes in the lamp socket.
7. Firmly insert the lamp into the socket.
8. Replace the filter access panel.
9. Replace the absorbance module.
Figure 44. Removing the Lamp

Replacing an Absorbance Module Filter
CAUTION: The optics assembly may be hot. Allow at least

five minutes for the instrument to cool before opening the
optics assembly.
Also, be careful when removing the filter access panel as

there is a possibility that the bulb may have broken.
To remove a filter:
1. Remove the absorbance module from the system.

2. Remove the filter access panel. The filters are mounted on the
filter wheel as shown in Figure 43.
3. Locate the filter that is to be removed.
4. Firmly grasp the exterior filter housing with a pair of needle nose
pliers (Figure 45).
5. Pull the filter out of the spring loaded slot.
Figure 45. Removing a Filter

To install a filter:
1. Remove the filter access panel. The filters are mounted on the
filter wheel as shown in Figure 43.
2. Locate the filter position in which the filter will be installed.
IMPORTANT: Filters must be installed in adjacent filter

positions, in the order of their wavelength. There must
not be any empty filter positions between the lowest
wavelength and highest wavelength filters.
Note: If a new filter is installed in a previously empty

position or if the wavelength is being changed, the
wavelength of the new filter must be entered. See page
45 for details.
IMPORTANT: The 405 nm filter must be installed in

Filter Position 1.
3. Firmly grasp the exterior filter housing with a pair of needle nose
pliers.
4. Push the filter into the spring loaded slot.
IMPORTANT: The bottom groove of the filter must be

firmly seated in the filter wheel. If the groove is aligned
with the spring on the filter wheel then the filter has
been installed incorrectly and will result in invalid
instrument performance.
6. Replace the absorbance module.

Replacing the Tubing
To replace the tubing:
1. Lift the rear edge of the metal panel at the front of the system
(Figure 46) to expose the tubing.
Note: The metal panel is hinged at the front. When

lifting the front panel, prevent it from dropping all the
way forward.
Side
Panel
Figure 46. Lifting the Front Panel
2. Remove the four Allen screws from the left side panel, using a
3 mm Allen wrench, and remove the panel.
3. A diagram of the hydraulic tubing for the DSX™ Automated
ELISA System is shown in Figure 47. Consult the diagram for
routing of the tubing.
4. Replace the tubing and fittings as required.
5. Replace the side panel and secure it using the Allen screws.
6. Close the front panel.

Aspirate Wash
Tubing Head
Dispense
Tubing
To Vacuum Source
Vacuum
Vacuum
Sensor
Tubing
Figure 47. Hydraulic Tubing Diagram

Cleaning the Wash Head
CAUTION: Always disconnect the power before servicing

the system.
To remove the wash head assembly:
1. Grasp the wash head assembly and lift it up from the retaining
cradle.
2. Lift the wash head and tubing clear of the instrument. If
necessary, remove the wash tubing and waste tubing from the
retainer clips at the front of the instrument.
To clean the wash head assembly:
1. Pass the Cleaning Wire through the inside of each aspirate pin
and dispense pin on the wash head.
Note: There are two sizes of Cleaning Wires. One is for

the aspirate pin, and the other is for the dispense pin.
2. Run a PURGE to rinse any material from the wash pins.
Note: It is a good practice to do a final purge with

deionized water. This can be programmed into the final
wash in an assay.
To replace the wash head assembly:
1. Position the wash head assembly back in the retaining cradle.

2. If necessary, replace the wash tubing and waste tubing into the
retainer clips at the front of the instrument.


Returning a Module for Service
Chapter 10 Service
If the instrument must be returned for service, it must be cleaned and

decontaminated if it has been in contact with potentially infectious body
fluids (including human blood), pathological samples, or toxic or radioactive
materials.
Warning: While the DSX alone does not present a

biohazard, the samples that are used and all parts and
consumables in contact with the samples must be
considered biohazardous.
Note: Refer to page 81 for cleaning and decontamination

instructions.
To return a module for service:
Note: Refer to page 82 for module removal instructions.
1. Contact the nearest THERMO LABSYSTEMS technical service

facility for return authorization.
2. Clean and decontaminate the module.
3. Fill out an Equipment in Transit form (Figure 48).
4. Pack the module and the Equipment in Transit form for
shipment.
5. After you receive a return authorization, ship the module to the
nearest THERMO LABSYSTEMS facility (see page 92).

Figure 48. Equipment in Transit Form

Limited Warranty
Limited Warranty
Warranty and Special Provisions
THERMO LABSYSTEMS products are fully guaranteed for one year against defects
in parts, materials, and workmanship. Defective parts and materials will be replaced
or, at the discretion of THERMO LABSYSTEMS, repaired at no charge for a period
of one year and labor required for such replacement or repair will be provided at no
charge for a period of one year, provided that the products are utilized and
maintained in accordance with the instructions in the applicable operating and
servicing manual, and provided further that the products have not, as determined
solely by THERMO LABSYSTEMS, been subject to misuse or abuse by the
Customer or other parties unrelated to THERMO LABSYSTEMS. THERMO
LABSYSTEMS makes no warranty, expressed or implied, as to the fitness of any
product for any particular purposes other than those purposes described in the
applicable operating and servicing manual, nor does THERMO LABSYSTEMS
make any other warranty, whether expressed or implied, including merchantability,
other than those appearing on the face hereof. Where THERMO LABSYSTEMS
guarantees any product, whether under this Warranty or as a matter of law, and
there is a breach of such guarantee, the Customer's only and exclusive remedy shall
be the replacement or repair of defective parts and materials, as described above.
This shall be the limit of THERMO LABSYSTEMS liability. Furthermore, THERMO
LABSYSTEMS shall not be liable for incidental or consequential damages. Failure
of the Customer to notify THERMO LABSYSTEMS of a claimed defect by registered
mail within thirty days of the discovery thereof shall constitute a waiver of any claim
for breach of warranty.
When a product is required by THERMO LABSYSTEMS to be installed by a
THERMO LABSYSTEMS engineer or technician, the period of this Warranty shall
begin on the date of such installation, provided, however, that any use of the product
prior to such installation shall, at the sole election of THERMO LABSYSTEMS. void
this Warranty. When installation by THERMO LABSYSTEMS personnel is not
required, the period of this Warranty shall begin on the date of shipment from
THERMO LABSYSTEMS. The period of this Warranty shall begin as described
above whether or not the product has been installed or shipped pursuant to a
purchase order, and any trail period shall be deducted from the Warranty period that
would otherwise apply under a subsequent placed purchase order for that product.
Limitation of Liability. Notwithstanding anything to the contrary contained herein, the
liability of SELLER (whether by reason of breach of warranty, breach of contract,
tort, or otherwise), including without limitation under any indemnification provision
contained herein, shall be limited to replacement of goods returned to THERMO
LABSYSTEMS which are shown to THERMO LABSYSTEMS ’s reasonable
satisfaction to have been nonconforming or to refund the purchase price, or, if not
paid, to a credit amount of the purchase price therefor.
THE FOREGOING WARRANTIES ARE EXCLUSIVE AND ARE GIVEN
AND ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES,
EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION, THE
IMPLIED WARRANTY OF MECHANTABILITY AND THE IMPLIED
WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE. NEITHER
PARTY SHALL BE LIABLE TO THE OTHER FOR ANY INCIDENTAL,
INDIRECT, SPECIAL, OR CONSEQUENTIAL DAMAGES.

Thermo Labsystems Contact Information
Thermo Labsystems Contact Information
Thermo Labsystems/Chantilly
14340 Sullyfield Circle
Chantilly, VA 20151-1683
Tel: 703 631-7800
800 288-2354
Fax: 703 631-7816
Email: [email protected]

Index
Index
Index
absorbance, 24 password, 50
accessories pipette module, 12
dilution plate, 8 pipetting
liquid waste container, 21 profiles, 13
standard/control rack, 1 plate drawer, 19
wash buffer containers, 19 positioning, 38
ambient drawer, 9 power ON, 42
Aspirate, 18 Purge, 19
aspiration height, 17 Purge, 18
assay definition, 30 return of a module, 91
blanking, 25 RS232, 35, 39
cleaning, 81 self-tests, 44
configuration options, 28 Soak, 18
consumables, 52 stop bits, 35
sample tips, 2 sweep height, 17
data bits, 35 test filter, 24
decontamination, 81 tubing, 87
dilution wash, 53
protocols, 14 unpacking, 38
Dimensions, 34 Unpacking, 37
Dispense, 18 UPS, 42
dispense height, 17 usage area, 38
DSX warning labels, 36
description, 5 Wash Container
illustration, 5 cleaning, 53, 72
Fill, 18 installing, 53
Filters refilling, 53, 72
Dual Mode, 24 wash head
installing, 85 cleaning, 89
Multiple Mode, 34 positions, 17
reference filter, 24 replacement, 89
Single Mode, 34 wash pins, 16
test filter, 24 wash module, 16
footprint, 38 wash pins, 16
lamp, 82, 83, 84 wash protocol
maintenance operations, 18
daily, 79 waste, 21
six-month, 79 Waste Container
weekly, 79 cleaning, 72
microplates emptying, 72
deep well, 14 wavelength, 24, 34
Move, 18 well top, 17
Operations menu, 30 worklist, 32
parity, 35

Index

Automated ELISA System: Operator's Manual

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What are the main hardware components of the DSX system?

What are the main hardware components of the DSX system?

What are the main steps involved in an ELISA assay using the DSX system?

What are the main steps involved in an ELISA assay using the DSX system?

DSX™ Automated ELISA System

Part No. 91000060

 2001 This document is the copyright of Thermo Labsystems and must

Table of Contents ......................................................................................................... i

DSX™ System Operator’s Manual i

Starting the System ........................................................................................... 43

ii DSX™ System Operator’s Manual

About this Manual

DSX™ System Operator’s Manual iii

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iv DSX™ System Operator’s Manual

Figure 1. The DSX™ Automated ELISA System

DSX™ System Operator’s Manual 1

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• Different assays can be run on the same plate

DSX™ System Operator’s Manual 3

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4 DSX™ System Operator’s Manual

CAUTION: Power is on to the system and to the incubator

Wash Buffer/ Pipette Work System Absorbance Wash Incubator

Figure 2. Location of Principal Hardware Components

DSX™ System Operator’s Manual 5

CAUTION: The electrical interlock for the system cover

CAUTION: The system cover attenuates the beam of the

CAUTION: Pinching hazard. Be sure that your hands and

Figure 3. System Cover

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Standards or controls are contained in the control rack, which contains up

Sample Dilution Reagent Reagent Tip Waste

Figure 4. The Workspace

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Sample tips used for pipetting samples and standards/controls are

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 Note: The microplate positions are numbered from 1 to 4. Refer to

Position 1 Position 2 Position 3 Position 4

Figure 5. The Ambient Drawer

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CAUTION: The vertical barcode scanner has a maximum

(Located on the Front (Located Next to the

Figure 6. Barcode Readers

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 Note: Whenever this manual refers to a microplate, it is implied

Figure 7. Plate Holder

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Microplate The microplate handler transfers a microplate

Figure 8. The Pipette Module

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 IMPORTANT: For any pipetting device, fluid properties

CAUTION: Changing the pipetting profile can affect

The pipetting profile used for samples is specified during definition of

 Note: Refer to page 68 for the procedure to define worklist

The pipetting system of the DSX™ Automated ELISA System includes

Sample Mixing During Dilution

DSX™ System Operator’s Manual 13

The dilution modes are described below:

Note: The microplate positions are numbered from 1 to 4. Refer to

Note: Whenever this manual refers to a microplate, it is implied

IMPORTANT: For any pipetting device, fluid properties

Note: Refer to page 68 for the procedure to define worklist

Note: Refer to page 30 for a summary of the procedures to define

Note: The incubator modules are labelled as 1, 2, 3 and 4 and are

Note: Refer to page 18 for a summary of the wash protocol

Note: Select No Sweep for sweep mode and disable bottom

Note: Select No Sweep for sweep mode and disable bottom

Note: Be sure that the waste cap is securely tightened. Otherwise,

Note: A 10% (v/v) solution of household bleach in water is

Note: Procedures for specifying the wavelength mode and the

Note: The current version of Revelation™ software supports

Note: Procedures for specifying the manner in which assay results

Note: An assay must be created before it can be assigned to

Note: The procedure for running a worklist is summarized on

Note: Select the Options command on the Tools menu to view or