Figure 2. Chinese Bell Flower Abutilon Indicum Linn
Figure 2. Chinese Bell Flower Abutilon Indicum Linn
Figure 2. Chinese Bell Flower Abutilon Indicum Linn
More than 160,000 people affected worldwide shows 19 known cases of Zika virus
infection in the Philippines were caused by mosquito bites. (Department of Health, 2013) After
several doctors in the Philippine Children’s Medical Center contracted severe dengue, the
Department of Health said it was investigating the upsurge of dengue cases in the hospital.
(Cruz, 2018) Dengue cases breached 80,000 mark that is 80, 745 cases. Deaths from dengue also
increased to 496 cases from 424 cases in the previous years. (DOH, 2013) In 2015, there were
A chinese bell flower (Abutilon indicum linn.) commonly found native to tropic and
subtropical regions and sometimes cultivated as an ornamental, contains phytol and coumarin.
(Philippine Medical Plants, 2017) Scientific literature on essential oils had previously suggested
that phytol can play a repellent role. And coumarin has actually been commonly used as an
insect repellent forb many years—though it's never been marketed as one.(Handwerk,2015)
The study of Tsgratog, Sanguanpong, Unchalee, Grieco, Ngoen-Kluan, and
Chareonviriyaphap et al (2016) shows numerous plants with insect repelling properties are native
to the tropics where they are produced for a wide range of medicinal purposes. In Thailand, these
native plant species have a history of use for personal protection against biting insects. From our
some mosquito repellent properties. Of these, 9 plant species were characterized using an excito-
repellency test system against several Thai mosquito species. Results from these studies revealed
that five essential oils extracted from plants demonstrated promising insect repellent activity.
These active ingredients show promise for further development into formulations that may serve
Singh, B., Singh, P. J., and Mohanty, M.J. et al (2012) claimed that the mission to make
on mosquito behaviour and control. The search for the perfect topical insect repellent/killer
continues. This analysis was conducted to review and explore the scientific information on
review the following methodology was applied. By doing a MEDLINE search with key words of
selected plants, plant based insect repellents/killers pertinent articles published in journals and
authentic books were reviewed. The World Wide Web and the Extension Toxicity Network
database (IPCS-ITOX) were also searched for toxicology data and other pertinent
information. Repellents do not all share a single mode of action and surprisingly little is known
about how repellents act on their target insects. Moreover, different mosquito species may react
differently to the same repellent. After analysis of available data and information on the
ingredient, of the product in relation to medicinal uses, acute and chronic toxicity of the selected
medicinal plants, it can be concluded that the ingredients included in the herbal product can be
used as active agents against mosquitoes. If the product which contains the powder of the above
said plants is applied with care and safety, it is suitable fo use as a mosquito repellent/killer.
A study conducted by Gillij, Gleiser, Zygadlo, 2008 shows that mosquitoes are
with mosquitoes. Repellents based on essential oils (EO) are being developed as an alternative to
including toxic reactions, and damage to plastic and synthetic fabric. This work evaluated
the repellency against Aedes aegypti of EO from aromatic plantsthat grow in Argentina:
Minthostachys mollis, Rosmarinus officinalis, Tagetes minuta and Tagetes pusilla. Most EO
were effective. Variations depending on geographic origin of the plant were detected. At a 90%
12.5% B. spartioides, R. officinalis and A. citriodora showed the longest repellency times.
Comparisons of the principal components of each EO suggest that limonene and camphor were
A study about essential oils from fresh leaves of four aromatic plants viz., conducted by
globulus and Plectranthus amboinicus were extracted by hydrodistillation. The test solutions
were prepared as 20% essential oil in ethanol and positive control as 20% DEET in ethanol.
Essential oil blend was prepared as 5% concentration. Nulliparous, 3-5-day-old female adult
Aedes aegypti mosquitoes were used for repellencyscreening as per ICMR protocol. The study
showed that the repellency of 20% essential oil of O. sanctum, M. piperita and P. amboinicus
were comparable with that of the standard DEET (20%) as no mosquito landing on the test was
observed up to 6 h. The E. globulus oil exhibited mosquito repellency only upto 1½ h.
Considerable mosquito landing and feeding was displayed in negative control. In the case of the
oil blend, no landing of mosquitoes was seen up to 6Â h as that of positive control. The results
showed that the essential oil blend from O. sanctum, M. piperita, E. globulus and P. amboinicus
could repel Ae. aegypti mosquitoes or prevent from feeding as in the case of DEET even at a
lower concentration of 5%. This study demonstrates the potential of essential oils from O.
as mosquitorepellents against Ae. aegypti, the vector of dengue, chikungunya and yellow fever.
A study of Maguranyi, S.K., Webb, C.E., Mansfield, S., Russell, R.C., et al (2009).
While the use of topical insect repellents, particularly those containing synthetic active
strategies emphasized in public health messages, there is a growing demand in the community
for alternative repellents, particularly those of botanical origin and thus deemed to be "natural."
This study evaluated the repellency of essential oils from 11 Australian native plants in 5% v/v
formulations against Aedes aegypti, Culex quinquefasciatus, and Culex annulirostris under
laboratory conditions. A blend of the top 3 performing oils was then compared with deet and a
commercially available botanical insect repellent. All essential oils provided at least some
protection against the 3 mosquito species, with the longest protection time (110 min) afforded by
Prostanthera melissifolia against Cx. quinquefasciatus. Mean protection times against Ae.
aegypti were substantially lower than those for the Culex spp. tested. Deet provided significantly
longer protection against Ae. aegypti than both the 5% v/v blend of Leptospermum petersonii,
Prostanthera melissifolia, and Melaleuca alternifolia (the 3 most effective oils) and the
commercial botanical repellent. The results of this study indicate that these essential oils from
Australian native plants offer limited protection against biting mosquitoes and that a blend of
essential oils holds may offer commercial potential as a short-period repellent or under
conditions of low mosquito abundance. However, it is important that public health messages
continue to emphasize the greater effectiveness of deet-based repellentsin areas with risks
of mosquito-borne disease.
Study of Adams, Wongchai, Chaidee, Pfeiffer et al (2016) shows Plant essential oils have
been suggested as a promising alternative to the established mosquito repellent DEET (N,N-
designed a new audiovisual assay of repellent activity against mosquitoes "Singing in the Tube,"
testing single mosquitoes in Drosophila cultivation tubes. Statistics with regression analysis
should compensate for limitations of simple hardware. The assay was established with female
Culex pipiens mosquitoes in 60 experiments, 120-h audio recording, and 2580 estimations of the
distance between mosquito sitting position and the chemical. Correlations between parameters of
sitting position, flight activity pattern, and flight tone spectrum were analyzed. Regression
analysis of psycho-acoustic data of audio files (dB[A]) used a squared and modified sinus
Application of logistic regression defined the repelling velocity constant. The repelling velocity
(Cymbopogon nardus), tea tree (Melaleuca alternifolia), clove (Syzygium aromaticum), lemon
(Citrus limon), patchouli (Pogostemon cablin), DEET, cedar wood (Cedrus atlantica). In
conclusion, we suggest (1) disease vector control (e.g., impregnation of bed nets) by
simple mosquito repellency testing in Drosophila cultivation tubes, (3) automated approaches
and room surveillance by generally available audio equipment (dB[A]: ISO standard 226), and
(Lauraceae) were tested in the field near Vientiane city, Lao PDR, on humans
for repellent activity against mosquitoes. Landing mosquitoes were collected and later identified.
The most abundant mosquitoes captured belonged to the genera Armigeres, Culex, and Aedes.
All the plant oils tested at concentrations of 1.7 microg/cm(2), 3.3 microg/cm(2), and 6.3
microg/cm(2) were significantly more mosquito repellent than the negative control. Croton oil
was significantly repellent against mosquitoes of the three genera at the highest (6.3
microg/cm(2)) concentration tested. Litsea oil was significantly repellent against Armigeres at all
(1.7 microg/cm(2), 3.3 microg/cm(2), and 6.3 microg/cm(2)) concentrations tested. Hyptis oil
was significantly repellent against Armigeres at 3.3 microg/cm(2) and 6.3 microg/cm(2) and
against Culex at 1.7 microg/cm(2) and 6.3 microg/cm(2). The oils were analyzed for chemical
content of volatiles, mainly terpenes. Main constituents were beta-pinene, sabinene, and 1,8-
cineol from oils of the green parts of H. suaveolens; alpha-pinene, beta-pinene, and alpha-
objective to determine repellent activity of hexane, ethyl acetate, benzene, chloroform and
quinquefasciatus (Cx. quinquefasciatus), Aedes aegypti (Ae. aegypti) and Anopheles stephensi
(An. stephensi). Methods Evaluation was carried out in a net cage (45 cm×30 cm×25 cm)
containing 100 blood starved female mosquitoes of three mosquito species and were assayed in
the laboratory condition by using the protocol of WHO 2005; The plant leaf crude extracts of C.
halicacabum was applied at 1.0, 2.5, and 5.0 mg/cm2 separately in the exposed area of the fore
arm. Only ethanol served as control. Results In this observation, the plant crude extracts gave
protection against mosquitobites without any allergic reaction to the test person, and also,
the repellent activity was dependent on the strength of the plant extracts. The tested plant crude
extracts had exerted promising repellent against all the three mosquitoes. Conclusions From the
results it can be concluded the crude extract of C. halicacabum was potential for controlling Cx.
A study by Pohlit, Lopes, Gama, Tadei and Neto et al (2011) shows that the use
of plant repellents against nuisance biting insects is common and its potential for malaria vector
control requires evaluation in areas with different level of malaria endemicity. The essential oils
of Ocimum suave and Ocimum kilimandscharicum were evaluated against malaria vectors in
species of plants used as insect repellents. Also, bioassays were used to evaluate the protective
potential of selected plants extracts against mosquitoes. Results The plantspecies mostly used
as repellent at night are: fresh or smoke of the leaves of O. suave and O. kilimandscharicum
(Lamiaceae), Azadirachta indica (Meliaceae), Eucalyptus globules (Myrtaceae) and Lantana
camara (Verbenaceae). The most popular repellents were O. kilimandscharicum (OK) and O.
suave (OS) used by 67% out of 120 households interviewed. Bioassay of essential oils of the two
Ocimum plants was compared with citronella and DEET to study the repellence and feeding
inhibition of untreated and treated arms of volunteers. Using filter papers impregnated with
malaria mosquito Anopheles arabiensis and Anopheles gambiae, including a nuisance mosquito,
Culex quinquefasciatus. High biting protection (83% to 91%) and feeding inhibition (71.2% to
92.5%) was observed against three species of mosquitoes. Likewise the extracts of
Ocimum plants induced KD90 of longer time in mosquitoes than citronella, a standard
botanical repellent. Mortality induced by standard dosage of 30 mg/m2 on filter papers, scored
after 24 hours was 47.3% for OK and 57% for OS, compared with 67.7% for citronella.
Most of the studies are relatively similar to each other when it comes to the procedures of
getting the active fractions that can repel mosquito..The current study explores if the variety of
will also show its potentiality to have active constiturents that can fight against mosquitoes as
reported in many studies published in literatures where its phytochemical analysis , physical and
chemical of the active constituents were mainly responsible for the Chinese Bell Flower as a