The document discusses large-scale production of enzymes. It covers various topics related to enzyme production including upstream and downstream processes. Some key points covered are:
- Submerged fermentation is commonly used for large-scale manufacturing of enzymes.
- Common upstream processes include selection of microorganisms, process development, and concentration/purification of enzymes.
- Downstream processes include immobilization techniques like absorption and adsorption to purify and recover enzymes.
- Important industrial enzymes produced at large scale include cellulases for biofuels, proteases like subtilisin for pharmaceuticals, and amylases for food industries.
The document discusses large-scale production of enzymes. It covers various topics related to enzyme production including upstream and downstream processes. Some key points covered are:
- Submerged fermentation is commonly used for large-scale manufacturing of enzymes.
- Common upstream processes include selection of microorganisms, process development, and concentration/purification of enzymes.
- Downstream processes include immobilization techniques like absorption and adsorption to purify and recover enzymes.
- Important industrial enzymes produced at large scale include cellulases for biofuels, proteases like subtilisin for pharmaceuticals, and amylases for food industries.
The document discusses large-scale production of enzymes. It covers various topics related to enzyme production including upstream and downstream processes. Some key points covered are:
- Submerged fermentation is commonly used for large-scale manufacturing of enzymes.
- Common upstream processes include selection of microorganisms, process development, and concentration/purification of enzymes.
- Downstream processes include immobilization techniques like absorption and adsorption to purify and recover enzymes.
- Important industrial enzymes produced at large scale include cellulases for biofuels, proteases like subtilisin for pharmaceuticals, and amylases for food industries.
The document discusses large-scale production of enzymes. It covers various topics related to enzyme production including upstream and downstream processes. Some key points covered are:
- Submerged fermentation is commonly used for large-scale manufacturing of enzymes.
- Common upstream processes include selection of microorganisms, process development, and concentration/purification of enzymes.
- Downstream processes include immobilization techniques like absorption and adsorption to purify and recover enzymes.
- Important industrial enzymes produced at large scale include cellulases for biofuels, proteases like subtilisin for pharmaceuticals, and amylases for food industries.
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Large – Scale Production of Enzymes
1. The inactive cells are advantageous for enzyme activity.
a) True b) False 2. Which of the following does not produces “Proteases”? a) Bacillus b) Rhizopus c) Mucor d) Bacillus coagulans 3. Cellulase is used in which type of industry? a) Food industry b) Paper industry c) Biofuel industry d) Chemical industry 4. Which of the following is not included in immobilization process? a) Absorption b) Adsorption c) Entrapment d) Affinity 5. In Upstream processes, process development increases the mRNA hydrolysis and proteolysis? a) True b) False 6. Which of the following is not an upstream process? a) Selection of a suitable enzyme b) Process development c) Concentration and primary purification of enzymes d) Large scale production 7. Which of the following purified enzyme is used in pharmaceutical industry? a) Subtilisin b) Novozym-435 c) Bromelain d) Asparaginase 8. Which type of fermentation is used for large scale manufacturing of enzymes? a) Solid-state fermentation b) Submerged fermentation c) Solid-Gas state fermentation d) Gas-state fermentation 9. Which enzyme was first produced industrially? a) Bacteria enzyme b) Yeast enzyme c) Fungal enzyme d) Streptomyces 10. The production of enzyme is mostly carried out by? a) Batch fermentation b) Continuous fermentation c) Fed-batch fermentation d) Semi-batch fermentation 11. Which is the inducer of enzyme invertase? a) Starch b) Sucrose c) Galactosidases d) Fatty acids 12. What do you mean by “Nutrient repression”? a) Inhibition of unwanted enzyme production b) Production of unwanted enzymes c) Inhibition of cell nutrients d) Production of waste 13. What is the method of screening? a) To improve the microbial strain b) To improve the growth of an enzyme c) To choose appropriate microorganisms for the desired enzyme d) To determine the optimum conditions for growth of microbes 14. System of reaction in which no reactant leaves reaction mixture is termed as ___________ a) Open system b) Closed system c) Semi-open system d) Partially closed system 15. Which of the following is true for single cell protein? a) Algae cannot be used in single cell protein b) It is produced through fermentation c) It does not contain carbohydrates and vitamins d) Its utilization increases environmental pollution