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    C-Reactive Protein (CRP) : Instruments

    Uploaded by

    Enrique Duarte
    This document provides guidelines for validating a C-reactive protein (CRP) latex turbidimetry assay on AU 400/640 instruments. It specifies reagent preparation details, instrument settings, calibration parameters, and stability information to facilitate validation of the CRP assay when software or reagent versions change.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    C-Reactive Protein (CRP) : Instruments

    Uploaded by

    Enrique Duarte
    42 views1 page
    This document provides guidelines for validating a C-reactive protein (CRP) latex turbidimetry assay on AU 400/640 instruments. It specifies reagent preparation details, instrument settings, calibration parameters, and stability information to facilitate validation of the CRP assay when software or reagent versions change.

    Original Description:

    crp au

    Original Title

    crp

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    This document provides guidelines for validating a C-reactive protein (CRP) latex turbidimetry assay on AU 400/640 instruments. It specifies reagent preparation details, instrument settings, calibration parameters, and stability information to facilitate validation of the CRP assay when software or reagent versions change.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    42 views1 page

    C-Reactive Protein (CRP) : Instruments

    Uploaded by

    Enrique Duarte
    This document provides guidelines for validating a C-reactive protein (CRP) latex turbidimetry assay on AU 400/640 instruments. It specifies reagent preparation details, instrument settings, calibration parameters, and stability information to facilitate validation of the CRP assay when software or reagent versions change.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    C-REACTIVE PROTEIN (CRP)

    Turbidimetry
    LATEX

    The application parameters comprised here constitute a guide to facilitate the validation of our reagents by
    the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

    Instruments: AU 400 / 640


    Reagent preparation
    Working Reagent: Pour the contents of a Latex vial into a Diluent bottle.
    Shake the Latex before pipetting. Mix thoroughly. Stable for 20 days at 2-8 ºC.

    Instrument settings
    Specific Test Parameters Min OD: Max OD:
    Sample: Volume 2 µL Dilution 0 µL L: -0.1 H: 2.5
    Reagents: R1 Volume 300 µL Dilution 0 µL Reagent OD limit:
    R2 Volume µL First L -0.1 First H 1.5
    Wavelength: Pri. 540 Sec. Last L -0.1 Last H 1.5
    Method: END
    Reaction slope: + Dynamic Range:
    Measuring Point 1: First 1 Last 7 L 0 H 150
    Measuring Point 2: First 0 Last 0 Correlation Factor:
    Linearity: % A 1 B 0
    No Lag Time: On-board stability period:

    L H
    7. None selected
    8. Out of range 0 5.0
    L H
    Panic Value Unit: mg/L Decimal places: 1

    Calibration Specific

    Calibration Type: AB Formula: Y=AX+B Counts: 2 Process: CONC.

    Cal. Nº OD CONC Factor/OD-L Factor/OD-H


    Point 1: # * -0.1 2.5

    * Enter the assigned standard value


    # User defined

    Version 0409

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