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    Phos

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    Enrique Duarte
    This document provides instrument settings and parameters for performing a phosphorus test using a spectrophotometric method with phosphomolybdate and UV detection. It specifies reagent preparation, instrument settings including wavelengths, reaction kinetics parameters, calibration details, and acceptable concentration ranges. Validation is recommended when software or reagent versions change.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Phos

    Uploaded by

    Enrique Duarte
    30 views1 page
    This document provides instrument settings and parameters for performing a phosphorus test using a spectrophotometric method with phosphomolybdate and UV detection. It specifies reagent preparation, instrument settings including wavelengths, reaction kinetics parameters, calibration details, and acceptable concentration ranges. Validation is recommended when software or reagent versions change.

    Original Description:

    phos au400

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    This document provides instrument settings and parameters for performing a phosphorus test using a spectrophotometric method with phosphomolybdate and UV detection. It specifies reagent preparation, instrument settings including wavelengths, reaction kinetics parameters, calibration details, and acceptable concentration ranges. Validation is recommended when software or reagent versions change.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    30 views1 page

    Phos

    Uploaded by

    Enrique Duarte
    This document provides instrument settings and parameters for performing a phosphorus test using a spectrophotometric method with phosphomolybdate and UV detection. It specifies reagent preparation, instrument settings including wavelengths, reaction kinetics parameters, calibration details, and acceptable concentration ranges. Validation is recommended when software or reagent versions change.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    PHOSPHORUS

    Spectrophotometric
    PHOSPHOMOLYBDATE/UV

    The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
    instrument. It is advisable to validate the use when there is any change in software or reagent versions.

    Instrument: AU 400 / 640


    Reagent preparation
    Reagent 1: Use the Reagent A.
    Reagent 2: Use the Reagent B.

    Instrument settings

    Specific Test Parameters


    Sample: Volume 3 µL Dilution 0 µL
    Reagents: R1 Volume 210 µL Dilution 0 µL Reagent OD limit:
    R2 Volume 90 µL First L -0.1 First H 0.1
    Wavelength: Pri. 340 Sec. 380 Last L -0.1 Last H 0.5
    Method: END
    Reaction slope: + Dynamic Range:
    Measuring Point 1: First 0 Last 27 L 0 H 20
    Measuring Point 2: First 0 Last 10 Correlation Factor:
    Linearity: % A 1 B 0
    No Lag Time: On-board stability period:

    L H
    7. None selected
    8. Out of range 2.7 4.5
    L H
    Panic Value Unit: mg/dL Decimal places: 1

    Calibration Specific

    Calibration Type: AB Formula: Y=AX+B Counts: 2 Process: CONC.

    Cal. Nº OD CONC Factor/OD-L Factor/OD-H


    Point 1: # * -9999 9999

    * Enter the assigned standard value


    # User defined

    Vers. 0206

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