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    Viva

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    Mythily Vedhagiri
    The document contains 15 questions related to various topics in histopathology and hematology. Some of the key topics covered include: - The components and purpose of urine analysis and microscopy - The basic structure and use of compound microscopes - The differences between benign and malignant tumors - Tissue processing techniques like manual paraffin processing and cryosectioning - Basic and special staining techniques like hematoxylin and eosin, trichrome, and acid fast staining - Antigen-antibody reactions and immuno electrophoresis - Identification of parasites like malaria and examination of blood samples for anemia and leukemia types - Components and functions of bone marrow

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    Viva

    Uploaded by

    Mythily Vedhagiri
    363 views3 pages
    The document contains 15 questions related to various topics in histopathology and hematology. Some of the key topics covered include: - The components and purpose of urine analysis and microscopy - The basic structure and use of compound microscopes - The differences between benign and malignant tumors - Tissue processing techniques like manual paraffin processing and cryosectioning - Basic and special staining techniques like hematoxylin and eosin, trichrome, and acid fast staining - Antigen-antibody reactions and immuno electrophoresis - Identification of parasites like malaria and examination of blood samples for anemia and leukemia types - Components and functions of bone marrow

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    Pathology and microbiology lab viva

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    The document contains 15 questions related to various topics in histopathology and hematology. Some of the key topics covered include: - The components and purpose of urine analysis and microscopy - The basic structure and use of compound microscopes - The differences between benign and malignant tumors - Tissue processing techniques like manual paraffin processing and cryosectioning - Basic and special staining techniques like hematoxylin and eosin, trichrome, and acid fast staining - Antigen-antibody reactions and immuno electrophoresis - Identification of parasites like malaria and examination of blood samples for anemia and leukemia types - Components and functions of bone marrow

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    363 views3 pages

    Viva

    Uploaded by

    Mythily Vedhagiri
    The document contains 15 questions related to various topics in histopathology and hematology. Some of the key topics covered include: - The components and purpose of urine analysis and microscopy - The basic structure and use of compound microscopes - The differences between benign and malignant tumors - Tissue processing techniques like manual paraffin processing and cryosectioning - Basic and special staining techniques like hematoxylin and eosin, trichrome, and acid fast staining - Antigen-antibody reactions and immuno electrophoresis - Identification of parasites like malaria and examination of blood samples for anemia and leukemia types - Components and functions of bone marrow

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 3

    VIVA QUESTIONS

    1. Urine physical and chemical examination (protein, reducing substances, ketones,


    bilirubin and blood)

    i. What is the pH of urine?


    ii. Why urine is yellow in colour ?
    iii. What are the abnormal constituents present in urine ?

    2. Study of parts of compound microscope .

    i. What is compound Microscope?


    ii. What is the principle of microscope?
    iii. What is resolution?
    iv. What is the use of immersion oil in microscopy?
    v. What are the types of light microscope ?

    3. Histopathological slides of benign and malignant tumours.

    i. Define Carcinogens.
    ii. Differentiate Benign and Malignant Tumor.
    iii. Define Neoplasm.
    iv. What is squamous cell carcinoma ?
    v. What is Mono clonality ?

    4. Manual paraffin tissue processing and section cutting (demonstration)

    i. Name some fixative agents to preserve tissue architecture.


    ii. What are the types of microtome ?
    iii. What is the principle of Haematoxylin and Eosin staining?
    iv. What is the purpose of Haematoxylin and Eosin staining?

    5. Cryo processing of tissue and cryosectioning (demonstration)

    i. What is Cryoproocessing?
    ii. What are the agents in cryo processing?

    6. Basic staining – Hematoxylin and eosin staining.

    i. What is the principle of Haematoxylin and Eosin staining?


    ii. What is the purpose of Haematoxylin and Eosin staining?
    iii. What is Haematoxylin?
    iv. What is Eosin?
    v. What are the applications of Haematoxylin and Eosin Staining?
    7. Special stains – cresyl fast Blue (CFV)- Trichrome – oil red O – PAS

    i. What is used to demonstrate the presence of glycogen molecules ?


    ii. What is the role of Cresyl fast blue stain?
    iii. What is PAS?

    8. Capsule stain.

    i. Name the methods to identify the presence of capsulated bacteria?


    ii. What is capsule?
    iii. What is the principle of Anthrone method of capsule staining?
    iv. What are smooth and rough colonies?
    v. Name some capsulated bacteria.
    vi. What is negative staining?

    9. Simple stain.

    i. What are the shapes of bacteria? Give some examples.


    ii. What is morphology?
    iii. Give some examples of acidic stains.
    iv. What is the principle of simple staining?
    v. What is basic staining?
    vi. What are the types of stains?

    10. Gram stain.

    i. What are the differences between gram positive and gram negative bacteria?
    ii. What is the principle of Gram's staining?
    iii. What is the role of mordant in gram's staining ?
    iv. What is used as decolourising agent in Gram's staining ?
    v. What is used as counterstain in Gram's staining ?
    vi. Name some gram positive and gram negative bacteria.

    11. AFB stain.

    i. What are the differences between Acid fast positive and Non Acid fast bacteria?
    ii. What is the principle of Acid fast staining?
    iii. What is used as decolourising agent in Acid fast staining ?
    iv. What is used as counterstain in Acid fast staining ?
    v. Name some Acid fast positive and Non Acid fast bacteria .

    12. Antigen Antibody Reactions- Immuno Electrophoresis

    i. What is an antigen?
    ii. What are antibodies? What are the types of antibodies?
    iii. What are antigen antibody reactions?
    iv. What is an Immuno Electrophoresis?
    v. What is the principle of Immuno Electrophoresis ?
    vi. What are the applications of Immuno Electrophoresis?
    vii. What are the types of Immuno Electrophoresis?

    13. Slides of malarial parasites, micro filaria and leishmania donovani.

    i. What is malaria?
    ii. What are the causative agent of Malaria?
    iii. Which malarial parasite is prevalent more in India?
    iv. What are the different morphological stages of malaria?
    v. What is Elephantiasis?
    vi. What are the causative agent of Elephantiasis?
    vii. What is kinetoplast?
    viii. What is promastigote?
    ix. What is an Amastigote ?
    x. What is Leishmania Donovanii?
    xi. What is the vector for Leishmanial transmission?
    xii. What is Kala Azar?

    14. Haematology slides of anemia and leukemia.

    i. What is an Anemia?
    ii. What are the causes of Anemia?
    iii. How Anemia can be classified?
    iv. Why RBC are getting sickle shaped in Sickle cell Anemia?
    v. Give some examples of Macrocytic Anemia.
    vi. What is the symptom of Leukemia?
    vii. List the various types of lymphocytes and their role in cancer prevention .
    viii. What is AML and CML?
    ix. What is ALL and CLL?

    15. Study of bone marrow charts.

    i. What is Bone Marrow?


    ii. What are the types of Bone Marrow?
    iii. What are Stem cells?
    iv. What is Haematopoiesis?
    v. What are monocytes ?
    vi. What are Neutrophils?

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