Scribd Logo
Upload

Welcome to Scribd!

  • 28 views

    Haematoxylin and Eosin Technique

    Uploaded by

    Mythily Vedhagiri
    This document describes the haematoxylin and eosin technique for staining tissue samples. It lists the solutions used, including haematoxylin, eosin, lithium carbonate, acid alcohol, and Scott's tap water. The method involves staining the sample in haematoxylin, differentiating it in acid alcohol, counterstaining it in eosin, and then dehydrating and mounting it. The results are nuclei stained blue-black, cytoplasm varying shades of pink, and specific tissues like muscle fibres and red blood cells stained different colors.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    Haematoxylin and Eosin Technique

    Uploaded by

    Mythily Vedhagiri
    28 views2 pages
    This document describes the haematoxylin and eosin technique for staining tissue samples. It lists the solutions used, including haematoxylin, eosin, lithium carbonate, acid alcohol, and Scott's tap water. The method involves staining the sample in haematoxylin, differentiating it in acid alcohol, counterstaining it in eosin, and then dehydrating and mounting it. The results are nuclei stained blue-black, cytoplasm varying shades of pink, and specific tissues like muscle fibres and red blood cells stained different colors.

    Original Description:

    HE staining

    Copyright

    © © All Rights Reserved

    Available Formats

    DOC, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document describes the haematoxylin and eosin technique for staining tissue samples. It lists the solutions used, including haematoxylin, eosin, lithium carbonate, acid alcohol, and Scott's tap water. The method involves staining the sample in haematoxylin, differentiating it in acid alcohol, counterstaining it in eosin, and then dehydrating and mounting it. The results are nuclei stained blue-black, cytoplasm varying shades of pink, and specific tissues like muscle fibres and red blood cells stained different colors.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    28 views2 pages

    Haematoxylin and Eosin Technique

    Uploaded by

    Mythily Vedhagiri
    This document describes the haematoxylin and eosin technique for staining tissue samples. It lists the solutions used, including haematoxylin, eosin, lithium carbonate, acid alcohol, and Scott's tap water. The method involves staining the sample in haematoxylin, differentiating it in acid alcohol, counterstaining it in eosin, and then dehydrating and mounting it. The results are nuclei stained blue-black, cytoplasm varying shades of pink, and specific tissues like muscle fibres and red blood cells stained different colors.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 2

    HAEMATOXYLIN AND EOSIN TECHNIQUE

    SOLUTIONS

    (i) Gills Haematoxylin

    Haematoxylin 6.0g
    Alluminium Sulphate 4.2g
    Citric Acid 1.4g
    Sodium Iodate 0.6g
    Ethylene Glycol 269ml
    Distilled Water 680ml

    (ii) Eosin

    Eosin Yellowish 1.0g


    Distilled Water 100ml

    (iii) Lithium Carbonate 1%


    Lithium Carbonate 1g
    Distilled Water 100g

    (iv) Acid Alcohol 1%


    70% Alcohol 99ml
    conc. Hydrochloric Acid 1ml

    (v)Scott's tap water

    In a beaker containing 1L distilled water, add 20g sodium bicarbonate


    and 3.5g magnesium sulphate. add a magnetic stirrer and mix thoroughly
    to dissolve the salts. Using a filter funnel, pour the solution into a
    labelled bottle

    METHOD
    1. Take sections to water.
    2. Place sections in haematoxylin for 5 minutes.
    3. Wash in tap water.
    4. 'Blue' sections in lithium carbonate or Scott's tap water.
    5. Wash in tap water.
    6. Place sections in 1% acid alcohol for a few seconds.
    7. Wash in tap water.
    8. Place sections in eosin for 5 minutes.
    9. Wash in tap water.
    10. Dehydrate, clear . Mount sections in DPX

    RESULTS
    Nuclei Blue-black
    Cytoplasm Varying shades of pink
    Muscle fibres Deep pinky red.
    Fibrin Deep pink
    Red blood cells Orange/red

    You might also like