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    Aspartate Aminotransferase (Ast/Got) : Instrument

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    Enrique Duarte
    This document provides guidelines for validating reagents for measuring aspartate aminotransferase (AST/GOT) using a continuous spectrophotometric assay on an ARCHITECT C8000 instrument. It specifies reagent preparation and storage, instrument settings, reaction definitions, calibrator levels and volumes, validity checks, and calibration intervals. The validation ensures proper performance when software or reagent versions change.

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    Aspartate Aminotransferase (Ast/Got) : Instrument

    Uploaded by

    Enrique Duarte
    78 views1 page
    This document provides guidelines for validating reagents for measuring aspartate aminotransferase (AST/GOT) using a continuous spectrophotometric assay on an ARCHITECT C8000 instrument. It specifies reagent preparation and storage, instrument settings, reaction definitions, calibrator levels and volumes, validity checks, and calibration intervals. The validation ensures proper performance when software or reagent versions change.

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    This document provides guidelines for validating reagents for measuring aspartate aminotransferase (AST/GOT) using a continuous spectrophotometric assay on an ARCHITECT C8000 instrument. It specifies reagent preparation and storage, instrument settings, reaction definitions, calibrator levels and volumes, validity checks, and calibration intervals. The validation ensures proper performance when software or reagent versions change.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    78 views1 page

    Aspartate Aminotransferase (Ast/Got) : Instrument

    Uploaded by

    Enrique Duarte
    This document provides guidelines for validating reagents for measuring aspartate aminotransferase (AST/GOT) using a continuous spectrophotometric assay on an ARCHITECT C8000 instrument. It specifies reagent preparation and storage, instrument settings, reaction definitions, calibrator levels and volumes, validity checks, and calibration intervals. The validation ensures proper performance when software or reagent versions change.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    ASPARTATE AMINOTRANSFERASE (AST/GOT)

    Continuous-spectrophotometric
    IFCC

    The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
    instrument. It is advisable to validate the use when there is any change in software or reagent versions.

    Instrument: ARCHITECT C8000


    Reagent preparation
    Working Reagent: Pour the contents of a Reagent B bottle into a Reagent A bottle. Mix gently.
    Stable for 2 months at 2-8ºC.
    Instrument settings

    General Conventional Units


    Assay AST Type Photometric Results Unit U/L
    Number ... Version 1 Assay Defaults Low Linearity 1,1
    High Linearity 500
    Reaction Definition
    Reaction Mode Rate Down Read Times Gender and Age specific Ranges
    Wavelength 340 / 0 Main 3 -- 13 GENDER AGE (Y) NORMAL
    Last Required Read 13 Flex __ -- __ Male 0-130 0 - 40
    Absorbance Range … / … Color Correction __ -- __ Female 0-130 0 - 40
    Sample Blank Type None Blank Read Time __ -- __ Either 0-130 0 - 40

    Reagent / Sample R1 R2 Reults Units U/L


    Reagent AST Reagent Volume 200 Decimal Places 1 (Range 0-4)
    Diluent Saline Water Volume __ __ Correlation Factor 1.0000
    Diluent Disp. Mode Type 0 Dispense Mode Type 0 Type 0 Intercept 0.0000

    Diluent Name Sample Water Dilution Factor


    Standard 10 1:1.00

    Validity Checks
    Reaction definition None
    Rate Linearity % ___
    Calibration
    Method Linear

    Calibrators and Volumes


    Calibrator Set Standard Calibrator Level Concentration Volume
    Blank 0.0 10
    Std. 1 (*) 10
    Std. 2 -- --
    Std. 3 -- --
    Std. 4 -- --
    Replicates 3 (Range 1-3) Std. 5 -- --

    Intervals Validity Checks


    Calibration Interval Full Interval 999 hours Blank Absorbance range: 0.0000 -- 0.0000
    Calibration Type Adjust Type None Span Absorbance Range: 0.0000 -- 0.0000
    Expected cal Factor : 0.00
    Expected Cal factor tolerance: %0
    (*) Enter the standard value
    … Values to be entered by the user
    Version 0707

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