Stereo Microscopes

Stereo
Microscopes
The Next Revolution in Microscopy
A Giant Step Forward in Stereo Microscopy
Nikon offers a broad range of stereo microscopes and accessories, including a research stereo
microscope system with the world’s highest zoom ratio, superb resolution and bright fluorescence
imaging. Also features other versatile parallel-optics type models suitable for various applications
and Greenough-type models that are user-friendly and affordable.

SMZ1270/
SMZ25 SMZ18 SMZ800N
1270i

Optical system Parallel-optics type

Zoom ratio 25:1 18:1 12.7:1 8:1

Zooming range 0.63-15.75× 0.75-13.5× 0.63-8× 1-8×

Total magnification*1 3.15-945× 3.75-810× 3.15-480× 5-480×

(with standard set*2) (6.3-157.5X) (7.5-135X) (6.3-80X) (10-80X)

Working distance*3 60mm 60mm 70mm 78mm

Image capture ○ ○ ○ ○

System expandability ○ ○ ○ ○

Embedded use ― ― ○ ○

2
 Index
Stereo Microscopes Accessories (for SMZ1270/1270i, SMZ800N,
SMZ745/745T, SMZ445/460, SMZ-2)
• SMZ25, SMZ18‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 4
• SMZ1270/1270i, SMZ800N‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 8 • Objectives, Auxiliary Objectives‥‥‥‥‥‥‥‥‥‥‥‥‥ 18
• SMZ745/745T‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 12 • Nosepieces, Tubes, Eye-level Riser,
Intermediate Tubes‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 19
• SMZ445/460, SMZ-2‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 13
• Stages, Observation Attachments‥‥‥‥‥‥‥‥‥‥‥‥ 20
• Illumination Systems‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 21
Accessories (for SMZ25, SMZ18)
• Stands‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 22
• Base Unit, Focus Unit, Stand/Focus Mount‥ ‥‥‥‥‥‥ 14
• Universal Table Stands/Focusing Mounts‥ ‥‥‥‥‥‥‥ 23
• Objective, Tubes, Nosepiece/Focus Mount Adapter,
Stage‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 15 Specifications/System Diagrams
• Controller, Darkfield Observation Accessory, Polarizing • System Diagrams (SMZ25/18)‥ ‥‥‥‥‥‥‥‥‥‥‥‥ 24
Observation Accessory, Epi-fluorescence Set‥ ‥‥‥‥‥ 16
• Specifications (SMZ25/18)‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 25
• Fiber Illuminator Set, Coaxial Illuminator,
• System Diagrams
Ring LED Illuminator‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 17
(SMZ1270/1270i/800N, SMZ745/745T)‥ ‥‥‥‥‥‥‥‥ 26
• Specifications‥ ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 28

Related Products
• Digital Cameras for Microscopes‥ ‥‥‥‥‥‥‥‥‥‥‥ 30
• Digital Microscope ShuttlePix‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 31
• Multi-purpose Zoom Microscopes
MULTIZOOM AZ100/100M‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ 31

SMZ445/
SMZ745/SMZ745T SMZ-2
SMZ460

Greenough type Optical system

7.5:1 4.4:1 / 4.3:1 5:1 Zoom ratio

0.67-5× 0.8-3.5× / 0.7-3× 0.8-4× Zooming range

3.35-300× 4-70× (8-35X)/ 4-120× Total magnification*1

(6.7-50X) 3.5-60× (7-30X) (8-40X) (with standard set*2)

115mm 100mm 77.5mm Working distance*3

○ (SMZ745T) ― ― Image capture

― ― ― System expandability

○ ○ ○ Embedded use

*1 Depends on the combination of eyepiece and objective lens *2 With a 10x eyepiece and a 1x objective
*3 With a 1x magnification without auxiliary objective
3
Parallel-optics type
Research Stereo Microscope

Evolutionary stereo microscope

Nikon has developed a stereo microscope that features a large zoom ratio of 25:1, high resolution and exceptional
fluorescence transmission capability. These models meet the increasing needs for imaging systems that span spatial
scales from single cells to whole organisms.

World’s widest zoom range and highest

resolution for a stereo microscope Bright, high-contrast fluorescent images
• First stereo microscope to offer a 25:1 zoom range (SMZ25) • Fly-eye lens ensures uniform brightness over the entire field
• Both eye paths boast numerical apertures (NA) of up to 0.156, of view even at the lowest magnifications
using the SHR Plan Apo 1x objective and SMZ25 • Breakthroughs in optical design mean significantly improved
signal to noise ratio and crystal clear fluorescent images

Automation and digital imaging Easy to use

• Motorized focus and zoom operation (SMZ25) • User-friendly remote control (SMZ25)
• Imaging Software NIS-Elements enables the use of multiple • Easy-to-operate slim LED DIA base with OCC illumination
imaging, processing and analysis modalities, including • Wide range of illuminators and accessories that
z-stack capture, time-lapse imaging and EDF image accommodate a variety of observation methods
generation

Motorized zoom model with the Manual zoom model providing advanced
highest zoom ratio and resolution in optical performance and incredibly bright
the SMZ series fluorescence at an attractive price
Model SMZ25 SMZ18

Type Motorized zoom Manual zoom

Observation Brightfield/Darkfield/Fluorescence/Simple polarizing

Zoom ratio 25:1 18:1

0.75x - 13.5x
Magnification range 0.63x - 15.75x
(with 0.75/1/2/3/4/5/6/8/10/12/13.5x click stops)

Maximum magnification 315x*1 270×*1

Maximum FOV ø70 mm*2 ø59 mm*2

Maximum NA of 0.312*3 0.3*3

*1: Using SHR Plan Apo 2x/ C-W10xB *2: Using SHR Plan Apo 0.5x/ C-W10xB *3: Using SHR Plan Apo 2x

4
 Remarkable resolution and the world’s widest zoom range

Dynamic zoom ratio of 25:1 SMZ25

0.63x
2x
5x
15.75x

An innovative optical system known as “Perfect Zoom Optics” offers

the world’s first zoom ratio of 25:1 (zoom range: 0.63x - 15.75x*;
*as of May 2013). The SMZ25 can seamlessly capture the entire
dish while simultaneously delivering microscopic details.

Auto Link Zoom (ALZ) supports seamless viewing at different scales SMZ25

ALZ automatically adjusts the zoom

factor to maintain the same field of
view when switching objective lenses.
This function enables seamless
switching between whole organism
imaging at low magnifications
and detailed imaging at high
magnifications.
SHR Plan Apo 0.5× SHR Plan Apo 2×
zoom 6× zoom 1.5×

Maintains FOV at total magnification of 3×

Superior resolution never before seen on a stereo microscope SMZ25 SMZ18

Newly developed SHR (Super High Resolution) Plan Apo series Comparison of resolution and color aberration by
objective offers a resolution of 1100LP/mm (observed value, using resolution chart
SHR Plan Apo 2x at maximum zoom). The 0.5x, 1x, or 1.6x lower SMZ25 Conventional model
magnification objectives deliver a bright field of view and brilliant
images with true-to-life colors.

5
Parallel-optics type
Bright, high-contrast fluorescent images SMZ25 SMZ18

Enhanced brightness and uniform illumination in a low magnification range

The SMZ25 series is the first stereo microscope in the world to use a fly-eye lens on
an epi-fluorescence attachment. This ensures bright, uniform illumination even at low New epi-fluorescence attachment
magnifications across a large field of view. Lens Lens

Improved S/N ratio and crystal clear fluorescent images thanks to Light source
an improved optical system

Nikon has succeeded in improving the signal and reducing noise in fluorescent
images by using a short-wavelength, high-transmission Fluor lens. This enables Fly-eye lens uniformly
illuminates the entire field
observations of cell division and samples with weak fluorescence, both of which are of view
difficult using conventional stereo microscopes.

Sample images
(Brightfield) (Fluorescence)

Fertilized mouse egg 2 days old Transgenic Zebrafish embryo, Board

Image courtesy of Kazuo Yamagata, Ph.D., Center for Tg (isl1-GFP)
Genetic Analysis of Biological Responses, Research Institute (using SHR Plan Apo 1x at zoom magnification of 6x with SMZ25)
for Microbial Diseases, Osaka University Image courtesy of Hisaya Kakinuma, Ph.D., Laboratory for
Developmental Gene Regulation, Developmental Brain Science
Group, RIKEN Brain Science Institute

Automation and digital imaging SMZ25 SMZ18

A wide range of digital imaging capabilities with the Digital Sight series
and NIS-Elements imaging software
Easily obtain the information required, such as Z drive position, zoom factor,
objective lens, filter cube and LED DIA brightness, by using the Digital Sight series
and NIS-Elements or Digital Sight series DS-L4 together with the microscope.

Detected observation condition/available control ◎：Detection and control of observation condition possible　◯：Detection of observation condition possible

SMZ25 SMZ18

• Motorized focus unit • Manual focus unit

• Motorized epi-fluorescence set (control box A) • Manual epi-fluorescence set (relay box and control box B)

DS-L4 NIS-Elements DS-L4 NIS-Elements

Zoom magnification ◯ ◎ ◯ ◯

Focusing ◯ ◎ ー ー

Objective (with nosepiece) ◯ ◯ ◯ ◯

Diascopic LED illumination stand

◯ ◎ ◯ ◎
(ON/OFF, light intensity control)

Fluorescence illuminator (light intensity control) ◯ ◎ ◯ ◎

Filter cube ◯ ◎ ◯ ◯

For other combinations, please confirm with Nikon.

* With NIS-Elements F (Free package), functions above are not available. Use D/Br/Ar.
6
 Improved observation efficiency
Easy-to-use OCC illumination SMZ25 SMZ18

The LED DIA Base with built-in OCC illuminator generates minimal
heat, consumes little power and has a long life. The illuminator also Comparison images (film)
enhances the contrast of uneven surfaces, such as those of film.

Conventional diascopic illumination OCC illumination

What is OCC illumination?

The OCC illuminator can be controlled using a slide lever. OCC stands for oblique coherent contrast, a form of oblique lighting method
Thanks to scales on the slide lever, the user can save and developed by Nikon. Compared to conventional diascopic illumination that
reproduce desired illumination levels. In addition, an OCC illuminates directly from below, OCC illumination applies coherent light to
plate can be inserted into the illumination unit from the front samples in a diagonal direction, adding contrast to colorless and transparent
and rear sides, so images with different shadow direction sample structures.
can be observed.

User-friendly remote control SMZ25

The remote control provides easy access to zoom and focus controls and is designed for
both right- and left-hand use. The remote control contains an LCD monitor with an adjustable
backlight that provides at-a-glance information about zoom factor, objective lens, filter cube
and LED DIA brightness. The brightness of the LCD monitor and
LED indicators is adjustable

LCD monitor with adjustable backlight provides

observation information.

On-axis imaging for digital images SMZ25 SMZ18

Easily switch between stereo position (stereoscopic view) and

mono position (on-axis view) when using the P2-RNI2 Intelligent Comparison of on-axis image and stereoscopic image
Nosepiece by simply moving the objective lens.

On-axis image Stereoscopic image

7
Parallel-optics type
Stereo Microscope

Incredible sharpness throughout a wide magnification range

These versatile stereo microscopes provide both excellent optical performance, such as high-magnification, high-zoom
ratio and high-resolution images, and advanced operability. The expandability of parallel optics makes these models
suitable for a wide range of applications.

Highest-in-class zoom ratio Easy to get results

• Highest-in-class zoom ratio of 12.7:1 (0.63 – 8x) with • Automatically detects magnification data in combination with
SMZ1270/1270i the digital camera control unit (SMZ1270i only)
•New WF series objectives optimized for wide viewfield • Nosepiece offers both widened magnification range and on-
observation at low magnification axis imaging
• Eyepiece tubes with various inclination angles and slim-type
stands minimize user fatigue during observation

High-quality images Expandable with a wide range of accessories

• High-level chromatic aberration correction provides sharp • A wide range of accessories are available, including eyepiece
images throughout the viewfield. tubes and stands that are equal to superior specification
stereo microscope models

Versatile stereo microscope with the The same as the SMZ1270 but equipped with Affordable model with improved
highest-in-class zoom ratio intelligent functions found in superior models operability and basic performance
8 (SMZ1270i with a trinocular tilting tube and nosepiece)
 Highest-in-class zoom ratio

Wide zoom range

The SMZ1270/1270i offers the highest- The SMZ800N comes with a 1 – 8x zoom range, with higher
in-class zoom ratio of 12.7x (0.63 – 8x). magnification than conventional models and enables high-
It offers both low-magnification wide resolution observation of 640LP/mm (using ED Plan Apo 2x/WF at
viewfield observation of the whole of a maximum zoom).
35 mm petri dish* during screening and
high-magnification observation of minute
cell structures Improved resolution of SMZ800N
SMZ1270/1270i enables
* with 1x objective at the lowest magnification. observation of the whole of a
35 mm petri dish.

Wide viewfield of SMZ1270/1270i

Ampulex

0.63x zoom SMZ800N Conventional model

8x zoom

Newly developed objectives

The newly developed WF series objectives offer uniformly bright

images even at low magnification and wide viewfield observation
with SMZ1270/1270i. In addition, a 0.75x objective is now
available, expanding the lineup of low magnification objectives.

High-quality images

Apochromat optics have been adopted for

the lenses in the SMZ1270/1270i zoom body
and semi-apochromat optics in the SMZ800N
to achieve high-level chromatic aberration
correction. They provide sharp images without
blur or color fringe.

Apochromat optics Conventional optics

(captured with SMZ1270+Plan Apo 1x/WF)
9
 Easy to get results

Intelligent function for status readout SMZ1270i

In combination with the Camera Control Unit DS-L4 and imaging software NIS-Elements, the SMZ1270i can detect zoom magnification
data. In addition, with the Intelligent Nosepiece P-RNI2 attached, data related to the objective in use is also detected. Calibration data is
automatically altered, following changes of magnification, to display the appropriate scale and measurement results on the images.

DS series camera
DS-Fi3/DS-Ri2*

Stand-alone Control Unit DS-L4

• Detection of zoom
magnification and objective
information
•Calibration auto change

SMZ1270i + Intelligent Nosepiece Imaging Software NIS-Elements

Correct scale is always indicated

On-axis observation with the nosepiece

The double nosepiece offers easy on-

axis imaging, enabling observation Without nosepiece On-axis imaging
of the bottom of holes, accurate
simple measurement and extended
depth of focus (EDF) imaging without
distortion.

On-axis view

Ergonomic design

Eyepiece tubes with a range of

Observation posture
inclination angles are available
for comfortable observation.
They offer the optimum eyelevel
200mm
to suit each user. In addition,
slim-type plain stands and the Inclination
LED Diascopic Illumination angle 20°

Stand easily facilitate the 434mm

presentation and removal of

specimens.
With the LED Diascopic Illumination Stand and Fiber Diascopic
Illumination Stand, focus control during observation is possible
using the dial in front of the base.
10
 Expandable with a wide range of accessories

In addition to conventional
accessories, the level of accessories
used with superior models is also
available for the SMZ1270/1270i and
SMZ800N. These include trinocular
tubes and slim-type LED diascopic
illumination stands. These allow
various microscope configurations Fibers Semiconductor Brain of adult drosophila excited with GFP
to suit numerous routine inspections Image courtesy of Hokto Kazama, Ph.D., Circuit
Mechanisms of Sensory Perception, Brain
and a range of research and Science Institute, RIKEN
development applications.

With the LED Diascopic

OCC illumination Diascopic brightfield illumination Illumination Stand and
Fiber Diascopic Illumination
Stand, image contrast
under OCC illumination can
be easily adjusted.

OCC illumination boosts the contrast of transparent sample structures.

Hemicentrotus pulcherrimus in two-cell stage

SMZ1270 SMZ1270i SMZ800N

Optical system Parallel-optics type (zooming type)

Zoom ratio 12.7 : 1 8:1
Zoom range 0.63 – 8x (0.63/1/2/3/4/6/8x stops) 1 – 8x (1/2/3/4/6/8x stops)
3.15 – 480x (depending on eyepiece and objectives) 5 – 480x (depending on eyepiece and objectives)
Total magnification
(with coaxial episcopic illuminator: 15 – 540x) (coaxial episcopic illuminator: 22.5 – 540x)
Tubes Eyepiece inclination: 20° (P-B Binocular Tube) / 0°-30° (P-TERG100 Trinocular Tilting Tube, P-TERG50 Trinocular Tilting Tube), P-T100 Trinocular Tube

Eyepieces C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7)

Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF,

Objectives Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF, ED Plan 1.5x/WF, ED Plan 2x/WF
ED Plan 1.5x/WF, ED Plan 2x/WF, Plan 1x, ED Plan 0.75x, Achro 0.5x

Working distance 70 mm (with Plan Apo 1x/WF) 78mm (with Plan 1x)
9.8 kg (with P-B Binocular Tube + P-DSL32 11.9 kg (with P-TERG100 Trinocular Tilting Tube +
Weight (approx.) 6.8 kg (with P-B Binocular Tube + C-PSN Plain Stand)
LED Diascopic Illumination Stand) P-DSL32 LED Diascopic Illumination Stand)
Please refer to the system diagram (P. 26-27) for accessory combinations.

Dimensions

SMZ1270 set SMZ1270i set SMZ800N set

SMZ1270 + P-B Binocular Tube + SMZ1270i + P-TERG100 Trinocular Tilting Tube + SMZ800N + P-B Binocular Tube +
Plan Apo 1x/WF + P-PS32 Plain Stand Plan Apo 1x/WF + Intelligent Nosepiece P-RNI2 + C-PSN Plain Stand
SMZ1000 P-DSL32 LED Diascopic Illumination Stand
160 173 160
140 Eyepoint level 199 160 Eyepoint level 140 Eyepoint level 199 130
(interpupillary (interpupillary distance: (interpupillary
distance: 64 mm) distance:
64 mm) 64 mm)
20°
146.5

133.5
217.5

411
434

407

326
338
338

52
62

160 Ø 60
244 Ø 60
136
78
70

70

244
27
35

35

292 305
300 337 300 337

Unit: mm

11
Greenough type
Greenough Type Stereo Microscope

Superior 7.5x zoom and 115 mm working distance

Trinocular optical head type is also available
• The SMZ745/745T boasts a 7.5x zoom that incorporates the Greenough
optical system. The zoom range of 0.67x to 5x provides a broad
observation range.
• As well as high zoom ratio and magnification, the SMZ745/745T offers an
unrivaled 115 mm working distance.
• The SMZ745T incorporates an optical path switching lever that enables SMZ745
easy switchover between eyepiece and camera. A DS series digital camera
SMZ745T
can be attached. (with a DS series digital camera)

Three “A” design (V)

2000
• Air-tight SMZ745
By making joints airtight, contamination from dust, oil, water and other
contaminants is prevented.
1000
Airtight construction: JIS Degrees of protection provided by enclosures IPX1
• Anti-mold SMZ745 SMZ745T *Except in Europe
Anti-mold design developed exclusively by Nikon ensures peace of mind when
the microscope is used in environments subject to high heat or humidity. 0 1 2
(sec)
• Anti-electrostaic SMZ745 SMZ745T
Static electricity built up within the microscope is discharged almost instantly, ensuring higher yields.
Antistatic function: 1000–10V, discharge within 0.2 sec.

Specifications
SMZ745 SMZ745T

Optical system Greenough type (zooming type) Greenough type (zooming type), trinocular tube
Zoom ratio 7.5 : 1
Zoom range 0.67-5x (with 0.67/1/2/3/4/5x stops)
Total magnification 3.35-300x (depending on eyepiece and auxiliary objective used)

Straight tube ー Built-in C-mount 0.55x magnification lens (F.N. 11), compatible with 2/3 in. or smaller CCD

Tubes Fixed type Eyepiece inclination: 45 ° Interpupillary distance adjustment: 52-75 mm

Eyepieces (with diopter adjustment) C-W 10xB (F.N. 22), C-W 15x (F.N. 16), C-W 20x (F.N. 12.5), C-W 30x (F.N. 7)

Auxiliary objectives G-AL 0.5x (W.D. 211 mm), G-AL 0.7x (W.D. 150 mm), G-AL 1.5x (W.D. 61 mm), G-AL 2x (W.D. 43.5 mm), G-AL ERG 0.77–1.06x (W.D. 102–48mm)
Working distance 115 mm (standard)

Airtight construction JIS Degrees of protection provided by enclosures IPX1 ー

Weight (approx.) 1.6 kg (body) 1.8 kg (body)
F.N.: Field Number

Dimensions

SMZ745 set SMZ745 + C-PSN Plain Stand/CN SMZ745T set SMZ745T + C-PSN Plain Stand/CN
164 150 164 140 45
45°

389
385

325

325

Ø60
Ø60
W.D.: 115
26 W.D.: 115

159 159
135
26

292 305 292 305 Unit: mm

12
 Greenough Type Stereo Microscope

Designed for excellent cost

performance
• The SMZ445 has a 45° eyepiece tube inclination, and the SMZ460 has a 60° eyepiece tube
SMZ460 with C-FMBN
inclination, which is ideal for embedded use. Focusing Mount BN
• Compact design with ease-of-use and high optical performance.
• ESD protection guards against electrostatic damage to samples.
SMZ445 with
C-PSN Plain Stand/CN
Specifications
SMZ445 SMZ460
Optical system Greenough type (zooming type)
Zooming ratio 4.4 : 1 4.3 : 1
Zooming range 0.8–3.5x 0.7–3x
Total magnification 4-70× 3.5-60×
Eyepiece inclination: 45° Interpupillary distance adjustment: 54–75 mm Eyepiece inclination: 60° Interpupillary distance adjustment: 54–75 mm
Tube
Eyepiece diopter adjustable for both eyes Eyepiece diopter adjustable for both eyes
Eyepieces SM 10xB (F.N. 21), SM 15xB (F.N. 14), SM 20xB (F.N. 12)
Auxiliary objectives (option) AL5 (0.5x), AL7 (0.7x)
Working distance 100 mm (standard)
Weight (approx.) 1.0 kg (body) 1.1 kg (body)

Dimensions

SMZ445 SMZ445 + C-PSCN Compact Stand/CN SMZ460 SMZ460 + C-FMBN Focusing Mount BN
154 131 185 154 148
46 90 46

E.P
326

325.5

330

25

53
73

26
φ52

101
159
100

35
φ52
φ76 φ15.8
100
26.5

180 265 Unit: mm

Greenough Type Stereo Microscope

High-resolution optics ideal for inspection, assembly, and measurement

• Compact design with horizontally positioned zooming ring (rotation: 90º)
SMZ-2 (Clemmer is optional)
• Eyepiece inclination of 45° for comfortable observation

Specifications Dimensions
SMZ-2
120
Optical system Greenough type (zooming type)
Zooming ratio 5:1
Zooming range 0.8–4x
Total magnification 4–120x (Depending on eyepiece and auxiliary objective used.)
Tube Eyepiece inclination: 45° Interpupillary distance adjustment: 56–75 mm
280

Eyepieces (with SM E10xA (F.N. 23, standard), SM E15xA (F.N. 14), SM 20xB (F.N. 12),
diopter adjustment) C-W30x (F.N. 7)
Auxiliary objectives AL5 (0.5x), AL7 (0.7x)
Working distance 77.5 mm (with standard configuration)
77.5

Weight (approx.) 1.6 kg (body), 1.9 kg (stand)

24.3

190×144
Unit: mm

13
Accessories SMZ25 SMZ18

Wide range of dedicated accessories for SMZ25/SMZ18 for

all types of observation

Base Unit, Focus Unit, Stand/Focus Mount

Base Unit

Nikon has improved ease of use by moving the controls to the front of the base, including the brightness adjustment dial and the on/off switch.

Fiber DIA base Slim Bases

The Fiber DIA base features condenser lenses that can be switched The slimmer LED DIA Base and Plain Base help increase efficiency
between low and high magnifications. Furthermore, the OCC of sample manipulation by bringing the level of the sample closer to
illumination system allows high-contrast illumination. the table.

Conventional base

❷ P2-DBL LED Diascopic

Illumination Base

❸
❶ P2-DBF Fiber Diascopic Illumination Base
Slim base

❸ P2-PB Plain Base

Focus Unit Stand/Focus Mount SMZ18

The focus unit is combined with the base unit. Choose from either SMZ18 can be mounted on various compact stands using a focus
a manual or motorized focus unit. mount.

❹
❷
❷

❹ P-DSF32 Fiber Diascopic

❶ Illumination Stand
❶ P2-FMDN Focus Mount
❷ P-PS32 Plain Stand

❷ P2-FU Focus Unit

❸

❶ P2-MFU Motorized Focus Unit ❸ P-DSL32 LED Diascopic Illumination Stand

14
 SHR Plan Apo Objective Series
The SHR Plan Apo series features higher NA, wider field of view and superior flatness and color aberration correction.
These objective lenses can be seamlessly switched because all magnifications have the same parfocal distance. The bayonet mount design
allows lenses to be safely and easily removed.
SHR Plan SHR Plan SHR Plan SHR Plan
❸ ❹ Apo 0.5× Apo 1× Apo 1.6× Apo 2×

❷ SMZ25 0.078 0.156 0.25 0.321

❶ Maximum
NA
SMZ18 0.075 0.15 0.24 0.3

Working distance 71 mm 60 mm 30 mm 20 mm

3 mm water
Correction ring — — —
depth

Wavelength 380-700 nm

❶ P2-SHR Plan Apo 0.5x　　　❷ P2-SHR Plan Apo 1x

❸ P2-SHR Plan Apo 1.6x　　　❹ P2-SHR Plan Apo 2x

Tubes
Choose from two types of tilting trinocular tube and one type of low
eyelevel trinocular tube. All tubes have a camera port for seamless
integration with the Digital Sight series. ❸

❷
❶
❸ P2-TL100 Trinocular Tube L (eyepiece: port 100:0 / 0:100)

❷ P2-TERG50 Trinocular Tilting Tube (eyepiece: port 100:0/50:50)

❶ P2-TERG100 Trinocular Tilting Tube (eyepiece: port 100:0 / 0:100)

Nosepiece/Focus Mount Adapter Stage

Both single and double nosepieces are available. The stage features an XY stroke of 6x4* inches (150 mm x
100 mm) and can be attached to any of the bases, making it
❷ effective for capturing large images when used in combination with
imaging software NIS-Elements. A sliding stage and tilting stage are
❶
also available. *Limited Y travel with 32 mm column bases

❷ P2-FM Focus Mount Adapter

❶ P2-RNI2 Intelligent Nosepiece

P-SXY XY Stage

15
Accessories SMZ25 SMZ18

Remote Control
Nikon offers a remote control unit that can be used to
operate the microscope and capture images by hand. A
footswitch is also available, allowing the user to operate the ❶ ❷
microscope and capture images by foot, freeing the hands
for sample manipulation.

❶ AZ-PCR Photo Release

❷ AZ-FSW Foot Switch
P2-RC Remote Controller

Darkfield Observation Accessory Polarizing Observation Accessory

Darkfield viewing is The analyzer is attached
possible simply by to the objective and the
attaching the darkfield unit ❷ polarizer to the base or
to the base. stand to enable polarized
❶ P-DF LED Dark Field Unit ❶ viewing.
❷ Shading cover ❶P
 2-POL Simple Polarizing ❶
Attachment

Epi-fluorescence Set
Motorized Epi-fluorescence Set
The fluorescent turret can be operated using the ❼
❷ ❹
remote control or imaging software NIS-Elements.
❶
❶ P2-EFLM Motorized Epi Fluorescence Attachment
❷ Light shading Plate (comes with Fluorescence Attachment) ❺
❸ P2-EFL Filter Cube (GFP-B/GFP-L/RFP) ❸
❹ P2-EFLBF Filter Cube (Bright Field)
❻
❺ P2-CTLA Control Box
❻ P2-RC Remote Controller
❼ P2-CIA QL1x/0.5x 1/4λ Plate

Combinations with SMZ25

Manual Epi-fluorescence Set

An easy-to-use manual model for Nikon’s high- ❹ ❻
performance epi-fluorescence attachment. ❷
❶

❶ P2-EFLI Epi Fluorescence Attachment ❸

❷ Light shading Plate (comes with Fluorescence Attachment) ❺
❸ P2-EFL Filter Cube (GFP-B/GFP-L/RFP)
❹ P2-EFLBF Filter Cube (Bright Field)
❺ P2-CTLB Control Box
❻ P2-CIA QL1x/0.5x 1/4λ Plate

Combinations with SMZ18

16
 Fiber Illuminator Set
Flexible Double Arm Fiber Illumination Set Ring Fiber Illumination Set
The direction and angle of illumination can be changed to suit the This ring fiber illumination set features an episcopic illumination
sample by making adjustments with these double arms. The fiber unit that effectively captures images (can be used with 1x and 0.5x
holder position can also be changed to obtain the optimal position objective lenses).
for illuminating samples. ❶ P2-FIR Ring Fiber Illumination Unit
❶ C-FDF Flexible Double Arm Fiber Illumination Unit ❷ C-FLED2 LED Light Source for Fiber Illuminator
❷ C-FIDH Fiber Holder
❸ C-FLED2 LED Light Source for Fiber Illuminator

❶ ❷

❶
❸
❷

Combination with SMZ18 Combination with SMZ18

Coaxial Illuminator Ring LED Illuminator

The coaxial light illuminator makes it possible to view light reflected Ring LED illuminator is equipped with high-intensity, long-life (20,000
from the surface of a sample. It is ideal for shooting shadow-less hours) LEDs. The illuminator’s dial adjusts the intensity of the white
images of thick samples. LED.
❶ P2-CI Coaxial Epi Illuminator ❶ P2-FIRL LED Ring Illumination Unit
❷ C-FLED2 LED Light Source for Fiber Illuminator
❸ P2-CIA QL1x/0.5x 1/4λ Plate

❶
❷
❶
❸

Combination with SMZ18 Combination with SMZ25

17
Accessories

A variety of accessories are available

for stereoscopic observations

Plan Apo WF series

Objectives
A wide selection with various magnifications and working distances is available, including high-NA, high-resolution and wide-viewfield Plan
Apo WF series objectives with superior image flatness and chromatic aberration correction.

SMZ1270/1270i SMZ800N Working Zoom Actual

Objectives NA
distance (mm) magnification FOV*1

0.63x 0.0095 69.8

0.5x/WF 82
8x 0.0525 5.5

Plan 0.63x 0.0143 46.6

0.75x/WF 107
Apo 8x 0.0788 3.7
0.63x 0.0190 34.9
1x/WF 70
❶ ❷ ❸ ❹ ❺
8x 0.1050 2.8
0.63x 0.0285 23.3
1.5x/WF 44
❶ Plan Apo 0.5x/WF ❹ ED Plan 1.5x/WF 8x 0.1575 1.8
ED
❷ Plan Apo 0.75x/WF ❺ ED Plan 2x/WF Plan 0.63x 0.0380 17.5
❸ Plan Apo 1x/WF 2x/WF 35
8x 0.2100 1.4

*1 With C-W10xB eyepiece

SMZ800N Working Zoom Actual

Objectives NA
distance (mm) magnification FOV*1

1x 0.0145 44
Achro 0.5x 189
❶ Achro 0.5x 8x 0.0525 5.5
❷ ED Plan 0.75x 1x 0.0218 29.3
❸ Plan 1x ED Plan 0.75x 117
8x 0.0788 3.7
❶ ❷ ❸
1x 0.0290 22
Plan 1x 78
8x 0.1050 2.75

*1 With C-W10xB eyepiece

Auxiliary Objectives

Microscopes Auxiliary objectives Working distance (mm) Microscopes Auxiliary objectives Working distance (mm)

G-AL ERG 0.77–1.06x 102–48 AL5 (0.5x) 181

SMZ445/460
G-AL 0.5x 211 AL7 (0.7x) 127.5

SMZ745/745T G-AL 0.7x 150 AL5 (0.5x) 103

SMZ-2
G-AL 1.5x 61 AL7 (0.7x) 95
G-AL 2x 43.5

18
 Nosepieces
Double nosepiece with two-objective switchover. Easy changeover P-RN2 Nosepiece SMZ1270/1270i SMZ800N
from stereo position (stereoscopic view) to mono position (on-axis
view) is possible by simply moving the objective lens to the right. Observations with wider zoom
ranges are possible by simply
switching between two objectives.

P-RNI2 Intelligent Nosepiece SMZ1270i

Enables easy switchover between

two objectives. In combination
with the Digital Sight series digital
camera, it automatically detects
the data of objective in use.

立体視 view
Stereoscopic 垂直視
On-axis view

Tubes/Eye-level Riser SMZ1270/1270i SMZ800N

Various ergonomic tubes with different inclination angles enable suitable eye levels to be selected for observation, even when an intermediate
tube or illuminator is attached. Trinocular tubes are also equipped with camera ports.

P-B Binocular Tube P-TERG100/P-TERG50 Trinocular Tilting Tube

20° inclination angle allows Allows continuous adjustment of the

observation without having to lean eyepiece inclination from 0° to 30°.
forward and reduces fatigue during Optical path switching ratio of
long-time operations. eyepiece:camera port is 100:0/0:100
with P-TERG100 and 100:0/50:50 with
P-TERG50. P-TERG100 Trinocular Tilting Tube

P-T100 Trinocular Tube P-IER Eye-level Riser

The eyepoint height can be raised 106 mm Increases the eyepoint height 25 mm per riser for
by tilting the eyepieces 180° up. a total of 50 mm.
Optical path switching ratio of
eyepiece:camera port is 100:0/0:100.

Intermediate Tubes SMZ1270/1270i SMZ800N

Various intermediate accessories are available that can be inserted between the microscope zooming body and tube.

P-IBSS2 Beam Splitter S2 P-THSS Teaching Head P-IDT Drawing Tube

Using a beam splitter and camera adapter, Simultaneous observation of the same Drawing sample images is possible by
a digital camera can be attached to the viewfield is possible between the eyepiece simply tracing observed images that are
binocular eyepiece tube for imaging. lenses of both teaching head and overlaid on top of drawings within the
Optical path switching ratio of left microscope, making it ideal for educational viewfield. The drawings can be removed
eyepiece:right eyepiece:camera port is purposes. The pointer can indicate target from view by using the knob to block the
100:100:0/100:50:50. points in the viewfield during observation. light path.
19
Accessories
Stages
Stages allow smooth sample movement in order to change viewfield during observation.

C-SSL Dia-sliding Stage Circular Floating Stage 2

Used for diascopic observation, Used for episcopic observation.

this sliding stage can be easily Loaded with a sample, the stage
moved in the desired direction can be easily moved in the desired
simply with a light push. Travel direction simply with a light push
range is within ø38mm. to its edges. Travel range is within
SMZ1270/1270i SMZ800N ø40mm.
SMZ745/745T SMZ445/460 SMZ1270/1270i SMZ800N
Can be used with the SMZ25 and SMZ18 SMZ745/745T SMZ-2
SMZ445/460
Can be used with the SMZ25 and SMZ18

C-TRS Tilting Stage P-SXY XY Stage

This stage has a nonslip sheet The stage features an XY stroke

and can be tilted 30° from its of 150 mm x 65 mm. By attaching
horizontal position. AZ100 stage adapters, it
SMZ1270/1270i SMZ800N can be used for various
SMZ745/745T SMZ445/460 applications. It can be
SMZ-2 used with both diascopic
Can be used with the SMZ25 and SMZ18 and episcopic illuminators.
SMZ1270/1270i SMZ800N
SMZ745/745T SMZ445/460

Observation Attachments
Various observation accessories are available that utilize diascopic and episcopic illuminations. They can be used for samples that are difficult
to observe using standard illumination.

P-EFL Epi-fluorescence Attachment P-DF LED Darkfield Unit

Up to four epi-fluorescence filter cubes can be mounted. The fly- Equipped with the while light LED as the light source. Simply
eye lens provides bright illumination up to the viewfield periphery. placing the unit on the stage enables darkfield observation.
SMZ1270/1270i SMZ1270/1270i
SMZ800N SMZ800N
SMZ745/745T

C-POL Polarizing Attachment

Simple polarizing observation

is possible by placing the
polarizer on the stage while the
analyzer is attached to the tip
of the objective lens.
SMZ1270/1270i SMZ800N
SMZ745/745T

20
 Illumination Systems
Provides a cone of light from above the sample to the center, minimizing unwanted shadow.
Ring Illuminator
Suitable for observation of electronic substrates.

C-FIR Plastic Fiber-optics Ring LMS100 x 60-15W LED Ring Light SM-LW61Ji3 LED Ring Illuminator
Illuminator

Illuminator is located away from microscope. Color temperature is adjusted to 6500K Three types of covers are available (clear,
It enables bright observation with high- ± 500K to provide stable illumination. diffuser and opaque white). Anti-electrostatic
intensity light without damaging sample with Two types of covers are available. Anti- type
its heat. electrostatic type SMZ1270/1270i SMZ800N SMZ745/745T
SMZ1270/1270i SMZ800N SMZ745/745T SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460 ※ SMZ-2 ※

SMZ445/460 ※ SMZ-2 ※ SMZ445/460 ※ SMZ-2 ※

※ G-OBA60 Adapter is required.

Arm Illuminator/Episcopic The direction and angle of the illumination can be changed with simple adjustments of the
Illuminator flexible arm.

Arms for LED Episcopic Illuminator

SMZ-U Episcopic Arm G-EIA Flexible Arm

C-FID2 Double Arm Fiber C-FDF Flexible Double Arm C-LSL LED Episcopic Illuminator
Illuminator Fiber Illumination Unit

It enables bright observation with It enables bright observation with In combination with C-PSN Plain Stand/CN and C-PSCN
high-intensity light without damaging high-intensity light without damaging Compact Stand/CN, illumination angle flexibility is possible
sample with its heat. The direction sample with its heat. The direction from the back of the microscope.
and angle of illumination can be and angle of illumination can be By attaching arms, flexible change of direction and angle of
changed using the flexible arms. changed using the fiber holder. illumination is possible.
SMZ1270/1270i SMZ800N SMZ1270/1270i SMZ800N SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460
SMZ745/745T SMZ445/460 SMZ745/745T SMZ445/460
SMZ-2

Suitable for brightfield observation for high-reflectance flat surface samples such as polished metals
Coaxial Illuminator
and wafers.

P-CI Coaxial Episcopic Illuminator G-ICIL LED Coaxial Episcopic Illuminator

Coaxial illuminator for parallel optics-type Coaxial illuminator for Greenough-type stereo
stereo microscopes. Provides high-intensity microscopes. Equipped with both coaxial
illumination for the entire view field. episcopic and oblique illumination, which
*1/4λ plate is required illuminates from behind the microscope.
SMZ1270/1270i SMZ800N SMZ745/745T

21
Accessories
Stands

C-PSN
C-PSCN

C-PSN Plain Stand/CN, C-PSCN P-PS32 Plain Stand C-LEDS Hybrid LED Stand
Compact Stand/CN

Offers a comfortable work area and Features a slim design with a ø180 Both episcopic and diascopic
allows easy handling of samples. mm stage plate and 160 mm width observations are possible and can
C-PSCN has a small base that saves between the pillar and optical axis to be conducted simultaneously. The
desk space. boost working efficiency. space-saving built-in illuminator can be
switched and adjusted with ease.

Type Episcopic Episcopic Episcopic/Diascopic

Illumination
method － － Epi-oblique*, brightfield

Built-in filter － － －

Fine focus
knob － － －

Observation
magnification With all objectives, at all zoom ranges With all objectives, at all zoom ranges With all objectives, at all zoom ranges

SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460

Microscopes
P-PS32 can be used with the SMZ18.
* The illumination area is limited by conditions of use.

C-DS Diascopic Stand S P-DSL32 LED Diascopic P-DSF32 Fiber Diascopic

Illumination Stand Illumination Stand

Features a hand rest for comfortable The OCC illumination system allows Light source is located away
operation. Used in conjunction with colorless and transparent samples to be from microscope, enabling bright
C-DSLU LED Unit for Dia Illumination observed in high relief. Compact slim- observation with high-intensity light
Stand. type base enhances operation efficiency. without damaging sample with its heat.

Type Diascopic Diascopic Diascopic

Illumination Brightfield Brightfield, OCC** Brightfield, OCC**

method

Not required
Built-in filter － NCB11, ND4/16
(ø45 mm filter slot provided)
Fine focus
knob － Included Included

Observation 0.5x objective is compatible with zoom 0.5x objective is compatible with zoom
With all objectives, at all zoom ranges
magnification magnifications higher than 1.5x. magnifications higher than 1.5x.

SMZ1270/1270i SMZ800N SMZ745/745T SMZ445/460

Microscopes
P-DSL32 and P-DSF32 can be used with the SMZ18.　
** Conditions of use vary depending on objective in use.

22
 Universal Table Stands/Focusing Mounts

Universal Table Stands G-US1A/G-US2 Universal Table Stand P (US-3)

These stands are handy in microscopy with large samples not Not only can it be used for a large sample, but this extremely stable
loaded onto the standard stand. The microscope zooming body is stand also easily accommodates intermediate tubes.
mounted to the stand arm via a focusing mount. The G-US1A is a - Used in conjunction with the C-FMAN Focusing Mount AN on the SMZ1270/
table clamp type (table top thickness: 10 to 60 mm). 1270i/800N/745/745T/445/460.
- Used in conjunction with the C-FMBN Focusing Mount BN on the - Used in conjunction with the SM Focusing Mount on the SMZ-2.
SMZ1270/1270i/800N/SMZ745/745T/445/460.
- Used in conjunction with the SM Focusing Mount and the G-USA SM US Universal Stand P (US-3)
Adapter on the SMZ-2. 82.5
417.5
- Cannot be used with the SMZ1270/1270i/800N when intermediate tube is
mounted on these models.

G-US1A Universal Table Stand 1

30
413 133

452
25

φ38
φ28
364

φ28 410 315

213∼473
Can be installed on the top
or bottom edge.
Unit: mm
155

3∼53

Specifications
Universal Table Stand

G-US2 Universal Table Stand 2 Model G-US1A G-US2 P (US-3)

Vertical cross travel 245mm 229mm
413 133
Horizontal cross travel 260mm 272mm
Weight (approx.) 4.4kg 23.0kg 30.5kg

C-FMAN Focusing Mount AN — ○

25

C-FMBN Focusing Mount BN ○ —

C-FMCN Focusing Mount CN — —
364

SM Focusing Mount ○* ○
○: Possible * G-USA Adapter is required
125-385
G-USA Adapter
35 φ24.5
75

φ15.8

The image is a configuration

227
sample with the SM
Focusing Mount.
117
95

Unit: mm

Focusing Mounts

Various types of focusing mounts are available depending on use. They are used to incorporate stereo microscope bodies into IC bonders or
other devices (SM Focusing Mount is for SMZ-2). These mounts can also be used when attaching microscopes to Universal Table Stands.

C-FMAN Focusing Mount AN C-FMBN Focusing Mount BN C-FMCN Focusing Mount CN

SM Focusing Mount
101 148 160

30 25
50

74 100
25

53
101

φ24.5
35

φ32 φ76
ø76mm: C-FMAN Focusing Mount AN Ø15.8±0.03
ø62mm: SM Focusing Mount Unit: mm

C-FMAN Focusing Mount AN C-FMBN Focusing Mount BN C-FMCN Focusing Mount CN SM Focusing Mount

Focusing area 40mm 50mm 50mm 40mm

Weight (approx.) 0.6kg 0.8kg 1.6kg 0.6kg

Antistatic function 〇 〇 － －

Compatible microscopes SMZ1270/1270i/800N/745/745T/445/460 SMZ-2

23
System Diagrams (SMZ25/SMZ18)

DS-Ri2/Qi2
DS-Fi3
C-W10×B C-W15× C-W20× C-W30× 1/1.8" Camera 2/3” Camera C-mount CCD Camera
Eyepiece Eyepiece Eyepiece Eyepiece
C-mount Adapter C-mount Adapter C-mount
0.55× 0.7× TV Adapter A F-mount F-mount
Adapter Adapter
2.5×
A LV-TV
TV Adapter

AZ-PCR
Photo Release
A A A

P2-TERG100 P2-TERG50 P2-TL100 P2-RC

Trinocular Tilting Tube Trinocular Tilting Tube Trinocular Tube L AZ-FSW
Remote
Foot Switch
Controller
C-HGFI/C-HGFIE
HG Precentered Fiber Illuminator
P2-EFLM
Motorized Epi
Fluorescence Attachment
X (with Light Shading Plate)
P2-EFL
Filter Cube (GFP-B/GFP-L /RFP)

P2-EFLI
Epi Fluorescence P2-EFLC
Filter Cube Y
Attachment
X (with Light Shading Plate)
P2-EFLBF
Filter Cube
D (Bright Field) X P2-CTLA
Control Box
P2-CI
Coaxial Epi
Illuminator P2-MFU
Motorized Focus Unit
P2-FM
Focus Mount Adapter SU-AC AC Adapter
B

V X
W X C X P2-RLY
X P2-RNI2
SMZ18 SMZ25 Intelligent Nosepiece Relay Box
Zooming body Zooming body Y

V
P2-FU P2-CTLB
Focus Unit Control Box

B
W
P2-SHR Plan P2-SHR Plan P2-RLYC
Apo 0.5× Objective Apo 1× Objective P2-SHR Plan P2-SHR Plan Relay Cable
Apo 1.6× Objective Apo 2× Objective D
P2-CIA QL P2-CIA QL
0.5x 1/4λ 1x 1/4λ Plate
Plate

P2-FIRL P2-POL P2-FIR

LED Ring Simple Polarizing Ring Fiber Illumination Unit
Illumination Attachment Z AZLED-PCU Power Code
Unit
J
Z Power cable

P-DF AC Adapter 2
LED Dark Field Unit

C-FDF C-FLED2
Z Flexible Double Arm LED Light Source for Fiber Illuminator
Stage Adapters
for AZ Shading Fiber Illumination Unit
cover

C-SSL C-TRS
DIA Sliding Stage Tilting Stage C P2-FMDN
C-FIDH Focus Mount
Fiber Holder C-EP Extension Pillar
P-SXY64 V
XY Stage
H I G
B

H I J

H I H I
G
H I J
G G G
G
D

P2-PB P2-DBL LED Diascopic P2-DBF H I J

Plain Base Illumination Base Fiber Diascopic P-PS32
Illumination Base Plain Stand
G P-DSF32 Fiber Diascopic
Y AC Adapter 2 Illumination Stand

P-DSL32 LED Diascopic

Illumination Stand
AC Adapter 2

Power cable

24
Specifications/Dimensions (SMZ25/SMZ18)
Specifications
SMZ25 SMZ18
Zooming body
Optical system Parallel-optics type (zooming type), apochromatic optical system
Zoom Motorized Manual
Zoom ratio 25:1 18:1
Zoom range 0.63-15.75x 0.75-13.5x (with 0.75/1/2/3/4/5/6/8/10/12/13.5x click stops)
Aperture diaphragm Zooming body built-in
Objectives NA, WD (mm)
P2-SHR Plan Apo 2x 0.312, 20 (with a correction ring for water 0 to 3 mm in depth) 0.3, 20 (with a correction ring for water 0 to 3 mm in depth)
P2-SHR Plan Apo 1.6x 0.25, 30 0.24, 30
P2-SHR Plan Apo 1x 0.156, 60 0.15, 60
P2-SHR Plan Apo 0.5x 0.078, 71 0.075, 71
Total Magnification
3.15-315x (depending on objective used) 3.75-270x (depending on objective used)
(using C-W10xB eyepieces)
Eyepieces (F.O.V. mm) • C-W10xB (22) • C-W 15x (16) • C-W 20x (12.5) • C-W 30x (7)
• P2-TERG 100 Trinocular Tilting Tube (100/0 : 0/100)
Tubes (eyepiece/port) • P2-TERG 50 Trinocular Tilting Tube (100/0 : 50/50) Inclination angle : 0-30 degree
• P2-TL100 Trinocular Tube L (100/0 : 0/100) Inclination angle : 15 degree
Focusing Unit (stroke from • P2-MFU Motorized Focus Unit (up 96 mm/down 4 mm)
objective’s parfocal point) • P2-FU Focus Unit (up 97 mm/down 5 mm)
• P2-FM Focus Mount Adapter
• P2-FM Focus Mount Adapter
Focus mount adapter/nosepiece • P2-RNI2 Intelligent Nosepiece (2 objectives can be attached)
• P2-RNI2 Intelligent Nosepiece (2 objectives can be attached)
• P2-FMDN Focus Mount (for P-PS32/P-DSL32/P-DSF32 stands)
• P2-PB Plain Base • P2-DBL LED Diascopic Illumination Base (OCC illuminator built-in) • P2-DBF Fiber Diascopic Illumination Base
Bases/stand • P-PS32 Plain Stand (only for SMZ18) • P-DSL32 LED Diascopic Illumination Stand (OCC illuminator built in) (only for SMZ18)
• P-DSF32 Fiber Diascopic Illumination Stand (only for SMZ18)
Stages • P-SXY64 Stage • C-SSL DIA Sliding Stage • C-TRS Tilting Stage
Bright Field, Epi Fluorescence, Simple Polarizing (with P2-POL Simple Polarizing Attachment), Dark Field (with P-DF LED Dark Field Unit),
Observation methods
Oblique Lighting
4 filter cubes mountable, fly-eye lens built-in
Epi-fluorescence attachments
• P2-EFLM Motorized Epi Fluorescence Attachment • P2-EFLI Epi Fluorescence Attachment
Epi-fluorescence light sources • HG Precentered Fiber Illuminator Intensilight C-HGFIE HG/C-HGFI HG (130W)
• P2-FIRL LED Ring Illumination Unit
Episcopic illuminators Use with fiber light source
• P2-CI Coaxial Epi Illuminator • P2-FIR Ring Fiber Illumination Unit • C-FDF Flexible Double Arm Fiber Illumination Unit
Episcopic light source • C-FLED2 LED Light Source for fiber illuminator
32 kg 30 kg
(Motorized Epi Fluorescence Attachment configuration with (Epi Fluorescence Attachment configuration with Trinocular
Weight (approx.)
Trinocular Tilting Tube, Motorized Focus Unit, Intelligent Nosepiece, Tilting Tube, Focus Unit, Intelligent Nosepiece, LED DIA Base and
LED DIA Base and Objectives 1x and 0.5x) Objectives 1x and 0.5x)
30W
10W
(Motorized Epi Fluorescence Attachment configuration with Trinocular
Power consumption (approx.) (Epi Fluorescence Attachment configuration with Trinocular Tilting
Tilting Tube, Motorized Focus Unit, Intelligent Nosepiece and LED
Tube, Focus Unit, Intelligent Nosepiece and LED DIA Base)
DIA Base)

Dimensions
(configured with motorized epi-fluorescence (configured with epi-fluorescence (configured with plain stand and
attachment and LED DIA base) attachment and LED DIA base) focus mount)
298

298

300

514 25 514 25 337 (44)

302
244
569

569

492
471

471

429

444 444
Unit: mm

25
System Diagrams (SMZ1270/1270i, SMZ800N, SMZ745/745T)

SMZ1270/1270i/800N C
A SMZ1270i
SMZ800N
Zooming Body Zooming Body
SMZ1270
Zooming Body
B B B

D D D
● ●
P-TERG100 P-TERG50 P-T100 P-B
Trinocular Trinocular Trinocular Tube Binocular Tube P-RN2 Nosepiece
Tilting Tube Tilting Tube P-RNI2
Intelligent Nosepiece

Achro ED Plan Plan

0.5X 0.75X 1X

Plan Apo Plan Apo Plan Apo ED Plan ED Plan

0.5X/WF 0.75X/WF 1X/WF 1.5X/WF 2X/WF

E
Tubes with the ● symbol cannot be used in combination with stands with the ■ symbol (p. 27).

SMZ745/745T
A C-mount Camera

G-ICIL LED Auxiliary objectives

SMZ745 SMZ745T Coaxial Episcopic
Zooming Body Zooming Body Illuminator
D D G-AL 0.29x G-AL 0.5x G-AL 0.7x G-AL 1.5x G-AL 2x

Power cable
E

Eyepieces/Camera Adapters C-mount Camera

Rubber Rubber Horn-shaped

Eyeguard Eyeguard 25 Rubber Eyeguard Eyepieces C-0.55x DS C-0.7x Relay Lens C-mount
Relay Lens for DXM TV Adapter A
C-W10xB C-W15x C-W20x C-W30x

LV-TV Tube Adapter

C-FC Reticle C-FM Reticle A B

(Crosshairs) (Micrometer)

Intermediate Tubes B HG Precentered Fiber Illuminator "Intensilight"

P-IER Eye-level Riser Filter cubes

P-IBSS2 P-EFL GFP-B

Beam Splitter S2 GFP-L Filter Cube
P-EFLC Filter Cube
P-EFL Epi P-EFLBF Filter Cube (Bright Field)
Fluorescense ●
Attachment
P-CL QL1X 1/4λ Plate P-CL QL1.5X 1/4λ Plate

P-THSS Teaching Head

P-IDT Drawing Tube

Y-THPL LED Pointer Unit for Teaching Head

F TH-AC2 AC Adapter for TH Pointer

P-CI Coaxial Epi Illuminator Power Cable

P-THS Teaching Head Stand

Iris Diaphragm
Epi fluorescense attachments with the ● symbol cannot be used in
C P-CI QL1X 1/4λ Plate P-CI QL1.5X 1/4λ Plate
combination with stands with the ■ symbol (p. 27).

26
Illuminators
C-FID2 Double Arm Fiber Illuminator

C-LSL LED
Epi Illuminator

⑬ ④

G-EIA Flexible C-FDF Flexible Double Arm Fiber

Supporting Arm Illumination Unit
SMZ-U
Articulated
Arm AC Adapter 2

① ② ③ Power Cable ⑬
C-FLED2 LED
Light Source for
Fiber Illuminator AZ-LED
Power Codes
C-FIDH Fiber Holder
E
⑤

P-DF LED P-DF-LSP Light

C-POL Polarizing C-FIR Plastic Fiber-optics Dark Field Unit
Attachment Shielding Plate
Ring Illuminator
LMS100 x 60-15W ⑫
LED Ring Light
SM-LW61Ji3 LED C-LP 1/4λ Plate F ⑬
Ring Illuminator
⑩

Stands/Stages D

C-ER Extension Ring Adapter

for Achro 0.5x Objective

①
① ②
② ③
③

⑦⑩⑫
⑥⑧⑨ ④ ⑧

■ ■ ■
C-PSN Plain Stand/CN, C-LEDS Hybrid LED Stand C-DSLU LED Unit for
C-DS Diascopic
C-PSCN Compact Stand/CN Stand S ⑬ Dia Illumination Stand
Power Cable
①
① ②
C-FMAN Focusing
②
Mount AN ③ C-FMCN
Focusing Mount CN
C-FMBN
C-EP Extension Pillar
Focusing Mount BN (SMZ1270/1270i/800N: used with
auxiliary objectives 0.5x, 0.7x
SMZ745/745T: used with auxiliary
objectives 0.5x, 0.7x)

① ①
① ① ④
② ⑦⑨⑩⑪⑫ ④ ⑦⑩⑪⑫ ④ ⑦⑩⑪⑫ ⑤
⑤ ⑤
F

G-US1A Universal G-US2 Universal

Universal Table Stand P (US-3) Table Stand 1 Table Stand 2 P-PS32 Plain Stand P-DSL32 LED Diascopic
P-DSF32 Fiber Diascopic
Illumination Stand
Illumination Stand
AC Adapter 2

Power Cable
C-ASP2 Opal & C-GSP Glass Plate / Stage Adapters for AZ
SM Opal & Black SMZ Black Acrylic Plate C-GSP2 Glass Plate ⑥ ⑦
Acrylic Plate Glass Plate ESD Plate
Circular Floating
Stage 2*

⑧ ⑦ P-SXY
C-TRS Tilting * Cannot be used C-SSL DIA XY Stage
Stage
⑧ ⑨ observation.
for diascopic
Stands with the ■ symbol cannot be used in combination with tubes and Sliding Stage
⑩ ⑪
epi fluorescense attachments with the ● symbol (p. 26).

27
Specifications

Parallel-optics type

Model SMZ25 SMZ18

Optical system Parallel-optics type (zooming type)

Zoom ratio 25:1 18:1

Zoom range 0.63-15.75x 0.75-13.5x

Total magnification* (When coaxial

3.15-945x (12.5-472x) 3.75-810x (19-405x)
episcopic illuminator is attached)

Tubes P2-TERG 100 Trinocular Tilting Tube, P2-TERG 50 Trinocular Tilting Tube, P2-TL100 Trinocular Tube L

Eyepiece inclination P2-TERG 100/50: 0°-30°, P2-TL100: 15°

Interpupillary distance P2-TERG 100/50: 50 mm or wider

adjustment P2-TL100: 50–75mm

C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7)
Eyepieces
(with diopter adjustment)

Objectives P2-SHR Plan Apo 0.5x, P2-SHR Plan Apo 1x, P2-SHR Plan Apo 1.6x, P2-SHR Plan Apo 2x

Working distance (with standard

60 mm
configuration or 1x objective)

32 kg
Weight (approx.) (motorized Epi Fluorescence Attachment configuration) 10 kg (with Plain Stand and Ring LED set)

* Depending on eyepiece and objective used

Greenough type

Model SMZ745/745T SMZ445

Greenough type (zooming type)

Optical system Greenough type (zooming type)
Trinocular Tube (SMZ745T)

Zoom ratio 7.5 : 1 4.4 : 1

Zoom range 0.67–5x 0.8–3.5x

Total magnification* 3.35–300x 4–70x

Fixed (binocular tube: SMZ745,

Tubes Fixed
trinocular tube: SMZ745T)

Eyepiece inclination 45° 45°

Interpupillary distance
52–75mm 54–75mm
adjustment

C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7)
Eyepieces SM 10xB (F.N. 21), SM 15xB (F.N. 14), SM 20xB (F.N. 12)
(with diopter adjustment)

Objectives — —

G-AL 0.5x (W.D. 211mm), 0.7x (W.D. 150mm), 1.5x (W.D. 61mm),
Auxiliary objectives SM-AL 0.5x, 0.7x
2x (W.D. 43.5mm)
Working distance (with standard
115mm 100mm
configuration or 1x objective)

1.6kg (SMZ745 body)

Weight (approx.) 1.0kg (body)
1.8kg (SMZ745T body)
* Depending on eyepiece and objective used

28
 SMZ1270 SMZ1270i SMZ800N

Parallel-optics type (zooming type)

12.7:1 8:1

0.63 – 8x 1 – 8x

3.15 – 480x (depending on eyepiece and objectives) 5 – 480x (depending on eyepiece and objectives)
(with coaxial episcopic illuminator: 15 – 540x) (coaxial episcopic illuminator: 22.5 – 540x)

P-B Binocular Tube, P-T100 Trinocular Tube, P-TERG 100 Trinocular Tilting Tube, P-TERG 50 Trinocular Tilting Tube

P-B: 20°
P-T100: 10°
P-TERG100/50: 0°-30°

P-B: 48–75mm
P-TERG100/50: 50 mm or wider

C-W10xB (F.N. 22), C-W15x (F.N. 16), C-W20x (F.N. 12.5), C-W30x (F.N. 7)
(with diopter adjustment)

Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF, Plan Apo 0.5x/WF, Plan Apo 0.75x/WF, Plan Apo 1x/WF,
ED Plan 1.5x/WF, ED Plan 2x/WF ED Plan 1.5x/WF, ED Plan 2x/WF, Plan 1x, ED Plan 0.75x, Achro 0.5x

70 mm 78 mm

9.8 kg (with Binocular Tube + LED 11.9 kg (with Trinocular Tilting Tube +
6.8 kg (with Binocular Tube + Plain Stand)
Diascopic Illumination Stand) LED Diascopic Illumination Stand)

SMZ460 SMZ-2

Greenough type (zooming type)

4.3 : 1 5:1

0.7–3x 0.8–4x

3.5–60x 4–120x

Fixed

60° 45°

54–75mm 56–75mm

SM E10xA (F.N. 23, standard), SM E15xA (F.N. 14), SM 20xB (F.N. 12),
SM 10xB (F.N. 21), SM 15xB (F.N. 14), SM 20xB (F.N. 12)
C-W30x (F.N. 7)

— 0.8-4x

AL5 (0.5x, W.D. 103mm), AL7 (0.7x, W.D. 95mm)

SM-AL 0.5x (W.D. 181mm), 0.7x (W.D. 127.5mm)

100mm 77.5mm

1.1kg (body) 1.6kg (body), 1.9kg (Stand)

29
Related Products
Digital Cameras for Microscopes

Camera Head
Microscope Camera Monochrome Microscope Camera Microscope Camera

16.25-megapixel Color High-resolution 16.25-megapixel Monochrome Cooled 5.9-megapixel Color High-resolution

Enables a wide range of advanced digital

imaging capabilities using a PC

EDF（Extended Depth of Focus） option: Multichannel (multicolor)

Captures multiple high-resolution images at different focal depths to create a Multiple fluorescent channels can be captured in conjunction with other imaging
single extended depth of focus image or quasi-3D image. methods, such as OCC or brightfield.

Images captured
in single colors
Overlapping image with all colors

Individual cells resolved in a live drosophila embryo expressing GFP

Select the in-focus area and produce one and mCherry
all-in-focus image (Using SHR Plan Apo 2x at zoom magnification of 8x with SMZ25)
Image courtesy of Max V. Staller, Ph.D., Clarissa Scholes, and Angela DePace, Ph.D.,
Harvard Medical School
Time lapse

NIS-Elements makes it easy to set up a 0 sec 38.38 sec 57.14 sec 71.43 sec Calcium-imaging: Time-lapse imaging of
time-lapse imaging experiment. GCaMP expressing neurons inside a live
zebrafish shows individual neurons firing at
different times (arrowheads). The last time-
frame shows a whole cluster of neurons
(Using SHR Plan Apo 2x at zoom magnification firing (asterisk).
of 9x with SMZ25 and camera head DS-Qi1)
Image courtesy of Joe Fetcho, Ph.D., Cornell
University

Standalone Control Unit Offers an easy-to-use high-definition, large-touch-panel monitor

that can be used to quickly capture images without the use of a
PC or monitor.

Scene mode Various tools

Optimal imaging parameters for each sample type and observation method can Simple measurements of acquired image are possible, allowing lines and
easily be set using the icons. comments to be added to image data. In addition, data storage and output
functions for a wide range of applications are available.
Measurement function
Scene mode (bioscience) Scene mode (industrial)
Darkfield/fluorescence Wafer/IC-chip
Distance between Circle (perimeter/
Distance Perpendicular circle centers Angle Area Pitch distance
Differential interference/phase contrast Metal/ceramic diameter)

Board Scale display/positioning functions Drawing functions

Brightfield
HE staining FPD
Free curved
SCALE XSCALE GRID XY MEAS X HAIRS Count mark Text input
Enzyme labeled antibody method line

30
 Digital Microscope

Easy imaging

Step. 1 Turn on the power.

ShuttlePix provides 20x Step. 2 Adjust magnifications

optical zoom. Its magnification and focusing while observing
the monitor.
information is also linked to
ShuttlePix's scale and simple Step. 3 Press the image
measurement functions. capture button.

One touch EDF imaging

Begin imaging from a low sample Finish imaging at a high sample

area on the screen. area on the screen.

Others

Handy set Simple stand set

• A cordless body (built-in illuminator, compatible with • Simple reflection stand that requires no battery
SD card, battery-powered) • Diascopic LED stand enables diascopic
• Easy operation imaging
• Automatically uploads images to a PC

Multi-purpose Zoom Microscope

• Wide magnification range

• Various observation methods in the macro region are possible
depending on samples and purpose.

Wide magnification range Various observation methods

0.5x, 1x, 2x, 4x and 5x objectives are available. Used in combination with The AZ series mono-zoom mechanism enables true on-axis image
the AZ-W10x eyepiece and a coaxial episcopic illuminator, the AZ100 capture in the macro region. The AZ series supports a wide array of
series covers the full range of 5x to 500x magnifications. observation methods, including epi-fluorescence, reflected/transmitted
light brightfield, simple POL and differential interference contrast

Episcopic DIC Epi-fluorescence Diascopic simple Epi-fluorescence

polarizing and diascopic DIC

31
Optical Systems Parallel-optics type Greenough type
Parallel-optics type
(zooming type) Eyepieces
This system has a parallel optical path DS series
digital camera Eyepieces
into which various intermediate tubes,
including a beam splitter, coaxial
episcopic illuminator, epi-fluorescence
Prisms
attachment, teaching head, drawing tube
and eye-level riser, can be inserted.

Prisms
Greenough type
(zooming type)
Allows a compact body that is suited for
incorporation into other devices. Zooming mechanism

Objectives Objectives

Image used in page 2 composite image courtesy of Julie C. Canman, Ph.D., Columbia University

N.B. Export of the products* in this catalog is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedure
shall be required in case of export from Japan.
*Products: Hardware and its technical information (including software)
Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. September 2017
©2007-17 NIKON CORPORATION

WARNING TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

ISO 14001 Certified

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http://www.nikon.com/products/

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Printed in Japan (1709-06)T Code No. 2CE-ENAH-3 This brochure is printed on recycled paper made from 40% used material.
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