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DEPARTMENT OF BIOTECHNOLOGY
B. Tech
CURRICULUM
AND
SYLLABUS
REGULATION 2013 -14
RE
KALASALINGAM UNIVERSITY
(KALASALINGAM ACADEMY OF RESEARCH AND EDUCATION)
Anand Nagar, Krishnankoil - 626 126
B. Tech. BIOTECHNOLOGY REGULATION 2013
Institute Vision Institute Mission

To be a Center of Excellence of To Produce Technically Competent, Socially
International Repute in Education and Committed Technocrats and Administrators
Research through Quality Education and Research
Department Vision Department Mission
Building an undisputed reputation as a To provide well-crafted curricula in the various
frontrunner in teaching and research at branches of biotechnology and to inculcate the
the National and International levels requisite technical and research skills in
students so as to render them employable in
academia, and industries in the service of
society
Program Educational Objectives (B.Tech. - Biotechnology)
1. Graduates would have attained a general level of competence in order to pursue advanced
courses and / or acquire specialized training and skills relevant to their professions.
2. Graduates would be ready to take up responsible positions in Public and Private sector
undertakings to serve society through Academics, Industry and Law.
3. Graduates would have learnt to uphold ethical conduct in their professions, have effective
communication and managerial skills, and possess the urge to learn and update themselves
lifelong.
KALASALINGAM UNIVERSITY 2
Program Outcomes
Graduates will be able to
1. Understand the fundamental principles of molecular biology, biochemistry, genetics,
gene manipulation, gene transfer, chemical engineering and bioinformatics.
2. Analyze the problems in the production of biopharmaceuticals and agricultural
products as well as optimizing bioprocesses.
3. Recognize the importance of cleaning up the environment, preventing pollution and
optimizing the use of resources for sustainable development.
4. Identify, analyze and address complex biological and engineering problems associated
with biotechnology.
5. Design a method and apply the techniques of biotechnology towards the prevention,
diagnosis and treatment of hereditary and infectious diseases in humans, plants and
animals.
6. Communicate effectively in oral and written language with their peers, teachers and the
outside world.
7. Work individually and / or as part of a team towards the successful execution of their
individual and / or collective responsibilities.
8. Keep them updated on the modern trends and developments in the theory and
practice of biotechnology.
9. Grasp the essentials of managing various projects.
10. Perceive the importance of learning throughout their lives about developments in their
respective fields.
11. Recognize engineers as responsible technocrats who impact society through their
knowledge and actions.
12. Realize the importance of ethics and ethical behavior in their professional lives.
B. Tech. BIOTECHNOLOGY CURRICULUM

Semester I
Objectives &
Code
Subject Outcomes L T P C
No.
PEOs POs
HSS101 English for Technical Communication I 1,3 6,7,10 2 0 0 2
MAT103 Mathematics I 1,2 1,2,4 3 0 0 3
PHY131 Engineering physics I 1 1,2,3,4 3 0 0 3
CHY106 Engineering Chemistry 1,2 1,3,4 3 0 0 3
CSE102/ Programming Languages/Engineering 1,7,11/
1/1 2 0 0 2
MEC101 Drawing 4,7,10,11
Basic Electrical and Electronics
EEE101/ 3,4,10,11/
Engineering/ Basic Civil and 1/1 4 0 0 4
CIV101 6,7,9,10,11
Mechanical Engineering
PHY183/ Physics Laboratory/ Chemistry 1,3,4,11/
1/1 0 0 3 1
CHY182 Laboratory 1,2,3
CSE181/ Programming Language Laboratory/
1/1 1,4/7 0 0 3 1
MEC181 Workshop
Total 17 0 6 19
Semester II
Objectives &
Code No. Subject Outcomes L T P C
PEOs POs
6,8,9,10,1
HSS102 English for Technical Communication II 1,3 2 0 0 2
1
MAT104 Mathematics II 1,2 1,4 3 0 0 3
PHY112 Engineering Physics II 1 1,3,4 3 0 0 3
6,7,9,10,1
Basic Civil and Mechanical Engineering/
CIV101/ 1/
Basic Electrical and Electronics 1/1 4 0 0 4
EEE101 3,4,10,11
Engineering
CHY102 Environmental Sciences 1 3,4,9,12 3 0 0 3
4,7,10,11/
MEC101/ Engineering Drawing/ Programming
1/1 1,7,11 1 0 3 2
CSE102 Languages
1,4,5,6,8,
BIT103 Cell Biology and Genetics 1 3 0 0 3
10,11
MEC181/ Workshop/ Programming Language
1/1 7/1,4 0 0 3 1
CSE 181 Laboratory
CHY182/ Chemistry Laboratory/ Physics 1,2,3/1,3,4
1/1 0 0 3 1
PHY181 Laboratory ,11
Total 19 0 9 22
Semester III
Objectives &
PEOs POs
MAT202 Mathematics III 1,2 5 3 0 0 3
CHE253 Bioprocess Calculations 1,2 1,2 3 1 0 4
1,2,3,5,6,7,
BIT204 Microbiology 1,2 3 0 0 3
8,9,10,12
1,4,5,6,7,8,9
BIT209 Molecular Biology 1,2,3 3 0 0 3
,10,12
Analytical Techniques in 1,2,3,4,5,8,
BIT214 1,2 3 0 0 3
Biotechnology 10
BIT211 Principles of Biochemistry 1,2 1,3,11 3 0 0 3
BIT281 Biochemistry Laboratory 1,2,3 1,2,3 0 0 3 2
BIT283 Microbiology Laboratory 1,2,3 1,6,7 0 0 3 2
Cell and Molecular Biology
BIT286 1,2,3 3,5 0 0 3 2
Laboratory
Total 18 1 9 25
Semester IV
Objectives &
PEOs POs
HSSXXX Humanities Elective I 3 0 0 3
CHE252 Unit Operations 1,2 1,4 3 1 0 4
BIT203 Bioenergetics and Metabolism 1,2 1,2 3 1 0 4
1,2,6,7,8,9,
BIT205 Industrial Biotechnology 1,2,3 3 0 0 3
10,12
Bioinformatics and Computational
BIT215 1,2,3 1,8 3 1 0 4
Biology
BIT216 Protein Science and Engineering 1,2 1,4,5,8,12 3 0 0 3
BIT288 Computational Biology Laboratory 1,2,3 1,8 0 0 3 2
CHE291 Chemical Engineering Laboratory 1,2,3 1,7 0 0 3 2
Total 18 3 6 25
Semester V
Objectives &
PEOs POs
BITXXX Major Elective I 3 0 0 3
Minor Elective I 3 0 0 3
BIT303 Bioprocess Principles 1,2 2,11 3 1 0 4
1,2,3,5,6,7,8
BIT304 Genetic Engineering 1,2,3 3 1 0 4
,9,11,12
BIT322 Enzyme Technology 1,2 1,3,5 3 1 0 4
Reaction Engineering for
CHE357 1,2 1,2,4 3 0 0 3
Biotechnologists
1,2,3,4,7,8,
BIT387 Bioprocess Laboratory 1,2,3 0 0 4 2
10,11,12
BIT388 Genetic Engineering Laboratory 1,2,3 1,6,9 0 0 4 2
Total 18 3 8 25
Semester VI
Objectives &
PEOs POs
BITXXX Major Elective II 3 0 0 3
HSSXXX Humanities Elective II 3 0 0 3
Free Elective I 3 0 0 3
Minor Elective II 3 0 0 3
BIT305 Biochemical Engineering 1,2 1 3 1 0 4
BIT306 Immunology 1,2,3 1,2,5,8 3 1 0 4
1,4,5,6,7,
BIT389 Immunology Laboratory 1,2,3 0 0 3 2
11,12
BIT390 Biochemical Engineering Laboratory 1,2,3 1,2 0 0 6 2
Total 18 2 9 24
Semester VII
Objectives &
PEOs POs
HSSXXX Humanities Elective III 3 0 0 3
Free Elective II 3 0 0 3
BITXXX Major Elective III 3 0 0 3
BITXXX Major Elective IV 3 0 0 3
BIT401 Animal Biotechnology 1,2 1,2,5,8 3 0 0 3
BIT402 Plant Biotechnology 1,2 1,2,4,511 3 0 0 3
BIT403 Downstream Processing 1,2 1,2,4 3 1 0 4
BIT491 Downstream Processing Laboratory 1,2,3 1,2,4,5 0 0 6 2
Total 21 1 6 24
Semester VIII
Objectives &
PEOs POs
BITXXX Self study Elective 3 0 0 3
1,2,5,6,7,8,
BIT499 Project Work 1,2,3 0 0 26 10
9,10,11,12
Total 3 0 26 13
Total Credits (from 1st semester to 8th semester = 177)
LIST OF MAJOR ELECTIVES FOR III YEAR
Code No. Subject L T P C
BIT307 Environmental Biotechnology 3 0 0 3
BIT308 Spectroscopic Methods For Structure Determination 3 0 0 3
BIT309 Food Processing and Technology 3 0 0 3
BIT310 Pharmaceutical Biotechnology 3 0 0 3
BIT311 Healthcare Biotechnology 3 0 0 3
BIT313 Metabolic Engineering 3 0 0 3
BIT314 Drug Design and Development 3 0 0 3
BIT323 Exploring the microbial world 3 0 0 3
CHE352 Bioprocess Instrumentation and Control 3 0 0 3
CHE358 Transport Phenomena in Biological Systems 3 0 0 3
LIST OF MAJOR ELECTIVES FOR IV YEAR

BIT405 Nanobiotechnology 3 0 0 3
BIT406 IPR in Biotechnology 3 0 0 3
BIT407 Bioreactor Design and Analysis 3 0 0 3
B1T409 Cancer Biology 3 0 0 3
BIT410 Biomedical Engineering 3 0 0 3
BIT411 Bioresource Technology 3 0 0 3
BIT412 RNAi Technology 3 0 0 3
BIT413 Vaccinology 3 0 0 3
BIT417 Biosensors 3 0 0 3
BIT418 Molecular pathogenesis 3 0 0 3
BIT419 Molecular diagnostics and therapeutics 3 0 0 3
BIT420 Signal transduction 3 0 0 3
BIT421 Functional genomics 3 0 0 3
BIT422 Radiation biology 3 0 0 3
BIT423 Recombinant protein production 3 0 0 3
BIT424 Clinical trials and management 3 0 0 3
LIST OF MINOR ELECTIVES

CHE354 Mass Transfer 3 0 0 3
CHE355 Bioprocess Plant Design Economics 3 0 0 3
CHE356 Chemical and Bio-thermodynamics 3 0 0 3
CHE314 Colloids & Surface Science 3 0 0 3
CIV369 Environmental Impact Assessment 3 0 0 3
CIV463 Solid Waste Management 3 0 0 3
CIV416 Industrial Wastewater Management 3 0 0 3
CSE103 Data Structures 3 0 0 3
EIE409 Biomedical Instrumentation 3 0 0 3
EIE416 Optimization Techniques 3 0 0 3
INT 303 Database Management systems 3 0 0 3
LIST OF HUMANITIES ELECTIVES

HSS001 Total Quality Management 3 0 0 3
HSS002 Engineering Management 3 0 0 3
HSS004 Industrial Psychology 3 0 0 3
HSS005 Consumer Psychology 3 0 0 3
HSS006 Professional Ethics 3 0 0 3
HSS007 Operations Management 3 0 0 3
HSS008 Introduction to Economics 3 0 0 3
HSS010 International Trade and Finance 3 0 0 3
HSS011 Information Systems for Managerial Decision Making 3 0 0 3
HSS013 Cost Analysis and Control 3 0 0 3
HSS014 Introduction to Marketing Management 3 0 0 3
HSS016 Organizational Psychology 3 0 0 3
HSS017 International Economics 3 0 0 3
HSS018 Communication Skills 3 0 0 3
HSS022 Banking Theory and Practice 3 0 0 3
HSS023 Entrepreneurship Development 3 0 0 3
HSS025 Science Fiction: An Appreciation 3 0 0 3
HSS026 German - I 3 0 0 3
HSS028 French - I 3 0 0 3
Science Technology and Medicine in India:
HSS030 3 0 0 3
A Historical Perspective
HSS033 Modern Science in India 3 0 0 3
HSS035 History of Science and Technology 3 0 0 3
SEMESTER I
ENGLISH FOR TECHNICAL COMMUNICATION I L T P C
HSS101 (Common to all branches)
2 0 0 2
Course Outcomes:
At the end of the course, students would be able to
CO1: Participate in Non-verbal communication
CO2: Enjoy Cloze reading€ skimming and scanning
CO3: Frame simple sentences to express daily activities
CO4: Take notes when reading and listening lectures and media events
CO5: Frame Instructions, Recommendations and Short Speeches
CO6: Remember nuances of Note-making, the template of Notices, Advertisements, Graphs
and Charts
CO7: Write Short stories, anecdotes, process description, etc..
Course Outcomes Vs Program Outcomes Mapping:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 H M H
CO2 H M H M
CO3 H
CO4 H
CO5 H M
CO6 H
CO7 H
FOCUS ON LANGUAGE
Parts of speech - nominal compounds, noun phrases - relative pronoun - adjective -
numerical, comparison and contrast, collocation and word combinations - verb - preposition
and relative - conjunction- connectives, expressions of purpose and function, cause and effect
- articles - adjectives - sentence pattern - tenses - voice - rewriting the sentences in
impersonal/abbreviated passive grammatical structures - concord - sentence level verb noun
agreement - gerund - rewriting infinitive into gerund - imperative - rewriting imperative into
recommendation using should - word formation - varied grammatical function of the same
word - affixes - prefix and suffix, number prefix, negative prefix - reported speech - editing
strategies - conditional structures - real, unreal, no possibility, zero condition - writing formal
definition - abbreviation and acronym - idioms and phrases - varieties of English - British
versus American.
LISTENING SKILLS
Comprehension practice - vocabulary development - familiarity to varied types of spoken
English and accents - developing ability to understand audio and video media - aiming at
overcoming barriers to listening - listening to documentaries, radio news broadcasts, TV
news telecasts - active listening in discussions and to lectures - taking notes while listening -
extracting information from listening.
SPEAKING SKILLS
Oral practice - role play - interplay - seminar - transcoding visual into oral - participating in
short and longer conversation - voice record, replay, correction of intonation, pronunciation
and flow of speech - phonemes - vowels, consonants, stress, rhythm, intonation - group
discussion - participative learning - acquiring proficiency, fluency, accuracy in oral
communication - speaking practice - developing confidence - extempore speech - learning
professional/conversational etiquette.
READING SKILLS
Vocabulary extension - improving vocabulary - intensive reading - reading strategies -
identifying topic sentence - guessing meaning from content - picking out specific information
- professional reading - reading practice - predicting the content, critical and analytical
reading - reading articles in English newspapers, sports magazines, encyclopedias - reading
aloud, use of stress and intonation - reading and comprehending technical materials - cloze
reading.
WRITING SKILLS
Discourse cohesion - improving writing skills, avoiding common grammatical errors in
academic writing - extending the hints - writing shorter sentences - punctuation - dialogue
writing - paragraph writing, problems and solutions, achieving coherence, transition words,
sequence words - essays of descriptive and argumentative - writing instructions, use of
imperatives - jumbled sentences into sequential paragraph using linguistic clues - report
writing - technical reports, industry visit reports, events reports - writing recommendations -
letter writing - formal and informal letters - job application and resume, permission for in-
plant training, business correspondence letters, calling for quotation, placing order, lodging
complaint, persuasive letters - assignment writing - mini-project - transcoding - transferring
of information from text to pictorial/graphical representation and vice versa.
TEXT BOOK
1. Rizvi M Ashraf, Effective Technical Communication, Tata McGraw-Hill, 2005
REFERENCES
1. Daniel Jones, English Pronouncing Dictionary, Universal Book Stall, New Delhi, 17 th
Edition, 2000
2. Geoffrey Leech, Fan Svartvik, A Communicative Grammar of English, Pearson
Education Asia, 1994
3. Hornby, AS, Oxford Advanced Learner•s Dictionary of Current English, OUP, 7th
Edition, 2005
4. Manivannan G, English for Engineers - A Book on Scientific and Technical Writing,
Govi Publications, 2005
MATHEMATICS I L T P C
MAT103
(Common to all Branches) 3 0 0 3
Course Outcomes:
CO1: Find the Eigen values of a matrix and to use Cayley-Hamilton theorem for finding the
inverse of a matrix.
CO2: Explain the concept of curvature and to find envelope of a curve.
CO3: Apply partial derivatives to find maxima and minima.
CO4: Solve second order linear differential equations with constant coefficients, Cauchy•s
equation and Legendre•s equation .
CO5: Understand the geometry of sphere, plane and straight line in the three dimensional
space

CO1 M M
CO2 M M
CO3 M M M
CO4 M H
CO5 M M
MATRICES
Review of linear algebra - Matrix operations - Addition, scalar multiplication, multiplication,
transpose, adjoint and their properties - Special types of matrices - Null, identity, diagonal,
triangular, symmetric, Skew-symmetric, Hermitian, Skew-Hermitian, orthogonal, unitary,
normal - Rank - Consistency of a system of linear equations - Solution of the matrix equation
Ax = b ‚ Row - Reduced echelon form
EIGEN VALUE PROBLEMS

Eigen value and eigen vector of real matrix - properties of eigen values and eigen vectors -
Cayley - Hamilton theorem - Orthogonal transformation of a real symmetric matrix to
diagonal form - Reduction of quadratic form to canonical form by orthogonal transformation
- Index, signature and nature of quadratic form
DIFFERENTIAL CALCULUS
Review of limits - Continuity and differentiability - Curvature - Cartesian and Parametric co-
ordinates - Centre and radius of curvature - Circle of curvature - Evolutes - Involutes -
Envelopes - Partial differentiation - Euler•s theorem for homogeneous functions -Total
differential - Taylor•s expansion (two variables) - Maxima and Minima for functions of two
variables - Method of Lagrangian multiplier - Jacobians
THREE DIMENSIONAL ANALYTICAL GEOMETRY

Direction cosines and ratios - Angle between two lines - Equations of a plane - Equations of
straight line - Coplanar lines - Shortest distance between two skew lines - Sphere - Tangent
plane - Plane section of a sphere - Orthogonal spheres
ORDINARY DIFFERENTIAL EQUATIONS

Solutions of second and higher order linear ODE with constant coefficients - Cauchy•s and
Legendre•s linear equations - Simultaneous first order linear equations with constant
coefficients - Method of variation of parameters
TEXT BOOKS
1. Kreyszig, E, Advanced Engineering Mathematics, John Wiley and Sons (Asia)
Limited, Singapore , 8th Edn., 2001
2. Arumugam, S., Thangapandi Isaac, A., Somasundaram, A., Engineering Mathematics
Volume I, Scitech Publications (India) Pvt. Ltd., Chennai, 2nd Edn., Reprint 2000, 1999
REFERENCES
1. Grewal , B.S., Grewal, J.S., Higher Engineering Mathematics, Khanna Publishers,
New Delhi, 37th Edition., 5th Reprint 2004, 2003
2. Venkataraman, M. K., Engineering Mathematics First Year, The National Publishing
Company, Chennai, 2nd Edition., Reprint 2001, 2000
ENGINEERING PHYSICS I L T P C
PHY 131
Course Outcomes:
CO1: Understand the different types of sound waves and production & application of
ultrasonics.
CO2: Understand the basic concepts, production & applications of different types of laser
sources.
CO3: Know the general ideas about optical fibres and their applications in various fields.
CO4: Learn the basic knowledge of crystallography and it•s preparation techniques.
CO5: Gain the knowledge about the fundamentals, theory of quantum physics
CO6: Gain the knowledge about various mechanical properties & thermal properties of
matters.

CO1 M M M
CO2 H M M H
CO3 H H H H
CO4 H H H M
CO5 H H H M
ACOUSTICS AND STRUCTURE OF SOLIDS

Classification of sound ‚ reverberation ‚ Sabine•s formula ‚ common acoustical defects and
remedies ‚ classification of solids ‚ crystal structures ‚ X-ray diffraction ‚ crystal growth ‚
crystal defects
LASER AND FIBRE OPTICS

Interaction of radiation with matter ‚ quantum mechanical view ‚ three and four Level laser
system ‚ Holography ‚ construction and reconstruction of hologram ‚ Engineering and
medical applications ‚ introduction of fibre optics ‚ classification of fibre ‚ Engineering and
medical applications
QUANTUM PHYSICS
Inadequacy of classical mechanics ‚ Black body radiation ‚ Planck•s law ‚ Photo electric
effect ‚ Compton effect ‚ Einstein•s photoelectric equation ‚ Schrödinger wave equation ‚
particle in one ‚ three dimensional box.
NON-DESTRUCTIVE TESTING
Liquid penetrant ‚ magnetic particle and eddy current methods ‚ X-ray radiography ‚
fluoroscopy ‚ Gamma ray radiography ‚ ultrasonic scanning methods ‚ ultrasonic flaw
detector ‚ thermography.
RELATIVITY
Frame of reference ‚ Newtonian relativity ‚ Galilean Transformation equations ‚ Ether
hypothesis ‚ Michelson-Morley experiment ‚ special theory of relativity ‚ Lorentz
transformation equations ‚ length contraction ‚ time dilation ‚ relativity of simultaneity ‚
addition of velocities ‚ variation of mass with velocity ‚ mass-energy equivalence ‚
Minkowski•s four dimensional space ‚ time continuum.
TEXT BOOKS
1. P.K. Palanisamy, ƒEngineering Physics„, Scitech Publications (India), Pvt Ltd.,
Chennai, 2009.
2. S.O. Pillai and D.N. Sankar, A text book of Engineering Physics. New Age
International Publication, New Delhi, 2008.
REFERENCES
1. Murthy V.S.R., Jena AK, Gupta K.P. and Murthy G.S., Structures and Properties of
Engineering Materials, Tata McGraw ‚ Hill Publishing Company Limited, New Delhi,
2003.
2. Gaur R.K. & Gupta S.L., Engineering Physics, Dhanpat Rai publications (P) Ltd., New
Delhi, 2001.
3. Ali Omar. M, Elementary Solid State Physics, Pearson Education (Singapore), Indian
Branch, New Delhi.
4. William F. Smith, Foundations of materials science and Engineering, 3rd Edition, Tata
McGraw-Hill, New York, 2003.
5. Rajput B.S Pragati Prakashan, Advanced Quantum Mechanics, New Market, Begum
Bridge, Meerut.
6. Hand book of Electronics, Gupta S.L. Kumar V Pragati Prakashan, New Market,
Begum Bridge, Meerut.
7. Arthur Beiser, Concepts of Modern Physics - Tata McGraw ‚ Hill Publishing Company
Limited, New Delhi, 5th Edition, 2000.
L T P C
CHY 106 ENGINEERING CHEMISTRY
3 0 0 3
Course Outcomes:
CO1: Know the water quality parameters to assess the quality of water
CO2: Learn the techniques of purification of water
CO3: Gather the knowledge in basic concepts of thermodynamics
CO4: Explain the principles of chemical & electrochemical reactions and prevention of
corrosion of materials
CO5: Explain the principles and generation of energy in batteries, solar cells and fuel cells
CO6: Explain the preparation, properties and applications of polymers and nano-materials
CO7: Discuss the principles, instrumentations and applications of analytical techniques

CO1 H M
CO2 H
CO3 M
CO4 M
CO5 M
CO6 M
CO7 M M M
MACROSCOPIC PROPERTIES OF SYSTEMS IN EQUILIBRIUM

Basic concepts of thermodynamics- Mathematical form of First law and its limitations-
Enthalpy- Applications of first law (relation between Cp and Cv only) - Second law of
thermodynamics (Clausius and Kelvin statement) - Entropy changes for reversible and
isothermal processes - Problems-Entropy of phase transitions-Problems- Free energy and
work function, Gibbs-Helmholtz equation- Applications-Problems-Van•t Hoff isotherm and
isochore-Applications-Problems- Phase equilibria- Application to one component systems,
two component systems (eutectic and compound formation).
ELECTRODICS
Electrochemical series and its applications -Reference electrodes (H2 and calomel
electrodes)- Determination of single electrode potential by using reference electrodes - -EMF
measurements and its applications- problems- Nernst equation-Problems- Electrochemical
energy systems: primary and secondary batteries, fuel cells, solar cell- Chemical structure,
electronic behaviours and applications of conducting polymers.-Principles of chemical and
electrochemical corrosion - Corrosion control (Sacrificial anode and impressed current

methods).
DYNAMICS OF CHEMICAL PROCESSES

Basic concepts- Kinetics of parallel, opposing and consecutive reactions with examples-
Temperature dependence of rate of reactions-Problems -Techniques and methods for fast
reactions, flow techniques, relaxation methods and flash photolysis - Thermodynamic
formulation of reaction rates - Enzyme kinetics (Michaelis-Menten equation).
WATER TECHNOLOGY
Water quality parameters - Definition and expression - Importance and determination of
Dissolved oxygen (DO) content in water-Estimation of hardness (EDTA method)- Problems-
Determination of alkalinity- Water softening (zeolite) - Demineralisation (Ion- exchangers)
and desalination ‚ Boiler feed water-Domestic water treatment.
INSTRUMENTAL METHODS OF ANALYSIS

Fundamental principles, theory, instrumentation and applications of UV-Visible
spectroscopy, Gas Chromatography (GC), High Performance Liquid Chromatography
(HPLC), Thermogravimetric analysis (TGA), Differential Thermal Analysis (DTA),
Scanning Electron Microscopy (SEM), Tranmission Electron Microscopy (TEM),
Refractometry and Nephelometry.
TEXT BOOKS
1. Atkins P. W., Physical Chemistry, Sixth Edition, Oxford University Press, 1998.
2. Jain P.C. and Monica J., " Engineering Chemistry ", Dhanpat Rai Publications Co.,(P)
Ltd., New Delhi, 14th Edition 2002.
3. Sharma, B.K., "Instrumental Methods of Analysis ", Goel publishing House, 12th
Edition, 2001.
REFERENCES
1. Puri B. R., Sharma L. R., and Pathania M.S., ƒPrinciples of Physical Chemistry„,
Vishal Publishing Co., 2008.
2. Kuriakose, J.C. and Rajaram J., " Chemistry in Engineering and Technology ", Vol. I
and II, Tata McGraw-Hill Publications Co.Ltd, New Delhi ,1996.
3. Kund and Jain, " Physical Chemistry ", S. Chand and Company, Delhi, 1996.
4. Gordon M.Barrow, " Physical Chemistry ", Sixth Edition, Tata McGraw Hill, 1998.
5. Willard, H.H., Merritt. I.I., Dean J.A., and Settle, F.A., "Instrumental methods of
analysis", Sixth Edition, CBS publishers, 1986.
6. Vogel A.I., " Quantitative Inorganic Chemical Analysis ", V. Edition, 1989.
7. 7. Rouessac, F., " Chemical Analysis-Modern instrumental methods and techniques ",
Wiley- Publishers, 1999.
PROGRAMMING LANGUAGES L T P C
CSE 102
Course Outcomes:
CO1: Learn the basics of computer programming concepts using C programming language
CO2: Design programs involving decision structures, and loops
CO3: Understand how to include functions and structure as part of the solution
CO4:Utilize pointers & arrays to efficiently solve problems and understand the
dynamics of memory
CO5: Understand the file system and operations on files
CO6: Develop algorithms to solve basic programming problems & able to learnhands-on
experience in designing and implementing some selected types of team oriented
projects
CO7: Understand the UNIX basics and also the concept of Shell Programming

CO1 H M
CO2 M
CO3 M
CO4 M
CO5 H
CO6 M
CO7 M
BASIC ELEMENTS OF C & CONTROL STATMENTS

Introduction to C programming ‚ C character set ‚ Identifiers, keywords, data types,
constants, variable, declarations, expressions, statements, symbolic constants, Operators and
Expressions-Operator precedence and associativity of operators -Input and Output
Functions-Library Functions - Header Files - Simple Computational problems. Decision
Making: if statement - if-else statement - else-if ladder ‚ Looping statements ‚ While ‚ do-
while- Still more looping-For statement, Nested control statements- switch statement ‚ the
break statement - ? : Operator - Continue statement - goto statement ‚ Problems using
Control Structures.
USER DEFINED FUNCTION FUNCTIONS & STORAGE CLASSES

Need for User defined functions, a multifunction program- Elements of user defined
functions- Definition of Functions- Return values and their Types- Function Calls-Function
declaration-Category of functions- Nesting of functions ‚ Recursion- Problems on functions
& recursion functions. Storage Classes -Automatic Variables -External Variables ‚ Static and
Register Variables.
ARRAYS AND POINTERS

Defining and Processing an Array - Passing Arrays to Functions - Multidimensional Arrays -
Arrays and Strings - Enumerated data types-Programs using sorting, searching and merging
of arrays. Pointer Fundaments - Pointer Declarations - Passing Pointers to Functions -

Arrays and Pointers - Pointers and One-Dimensional Arrays - Pointers and Multidimensional
Arrays - Operations on Pointers-Programs using Pointers with Functions.
DYNAMIC MEMORY MANAGEMENT, STRUCTURES & UNIONS

Dynamic Memory Allocation ‚ Allocating a Block of memory, multiple blocks, releasing
used space, altering the size of block. ‚ Defining a Structure - Processing a Structure ‚ User
defined Data Types ‚ Nested structure - Structures and Pointers - Passing Structures to
Functions - Self Referential Structures- Arrays and & Structures Union.
DATA FILES AND UNIX OS

Opening and Closing a Data File - Creating a Data File ‚ Reading & writing a data file.
Processing and Updating of Data Files - Unformatted Data Files - Programs using merging,
searching of data file contents. Introduction to Operating System. Shell fundamentals- shell
commands ‚ File commands- Directory commands-Miscellaneous commands
TEXT BOOK
1. Byron S. Gottfried, Programming with C, Second Edition, Tata McGraw Hill, 2006
REFERENCES
1. Brian W. Kerninghan and Dennis M.Richie, ƒThe C Programming language„, Pearson
Education,2005.
2. Johnsonbaugh R.and Kalin M, ƒApplications Programming in ANSI C„, Third Edition,
Pearson Education, 2003.
3. E. Balagurusamy ƒ Programming in ANSI C„ fourth edition TMH 2008
4. V.Rajaraman ƒComputer Basics and C Programming„ PHI 2008
5. Stephen Kochan and Patrick Wood, UNIX Shell Programming, Third Edition, Pearson
education, 2003
BASIC ELECTRICAL AND ELECTRONICS L T P C

EEE101 ENGINEERING
4 0 0 4
(Common to Non-circuit Branches)
Course Outcomes:
CO1: Do the basic estimation of electrical quantities
CO2: Interpret the basic electrical and electronics circuits
CO3: Understand the DC and AC single phase and three phase fundamentals
CO4: Understand the working principle of various Electrical AC and DC machines
CO5: Get the knowledge about various Analog type measuring instruments and house
wiring.
CO6: Get the knowledge about basic semiconductor devices.
CO7: Get the knowledge about the application of basic Electronics devices for domestic and
industries

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MAGNETIC CIRCUITS
Magnetic circuits - definition of MMF, flux and reluctance - reluctance in series and parallel
- electromagnetic induction - Fleming•s rule - Lenz•s Law - Faraday•s laws - statically and
dynamically induced EMF - self and mutual inductance - coefficient of coupling - hysteresis
- eddy currents - analogy of electric and magnetic circuits - simple problems.
DC CIRCUITS AND AC CIRCUITS

Electrical quantities - resistors - inductors - capacitors - Ohm•s Law - Kirchoff•s Laws -
series and parallel circuits - analysis of DC circuits - mesh, nodal - simple problems.
Sinusoidal functions - phasor representation - RMS Effective values - form and peak factors -
RLC circuits - power and power factor - analysis of 3 phase AC circuits - simple problems.
ELECTRICAL MACHINES
Construction and principle of operation of DC machines - generator, motor - single phase
transformers - alternators - three phase and single phase induction motors.
MEASURING INSTRUMENTS AND WIRING CIRCUITS

Moving coil and moving iron instruments - dynamometer type wattmeter - induction type
energy meter.
Domestic wiring - accessories - types - staircase wiring - fluorescent tube circuits - simple
layout - earthing.
ELECTRONIC DEVICES
Basic concepts of PN junction diodes - zener diode - bipolar junction transistor - uni polar
devices - FET, MOSFET, UJT - thyristor - photoelectric devices.
ELECTRONIC CIRCUITS
Half wave and full wave rectifier - amplifier - oscillator - RC integrator and differentiator
circuits - diode clampers and clippers - multivibrators - schmitt trigger.
TEXT BOOKS
1. V.K. Mehta, ƒPrinciples of Electrical Engineering and Electronics„, S. Chand &
Company Ltd, 2008.
2. Kothari D P and Nagrath I J, "Basic Electrical Engineering", Tata McGraw Hill, 1991.
3. Mithal G K, Electronic Devices & Circuits, Khanna Publications, 1997.
REFERENCES
1. T. Thyagarajan, ƒFundamentals of Electrical and Electronics Engineering„, SciTech
publications (Ind.) Pvt. Ltd., 3rd Edition, October 2000.
2. Muraleedharan K.A, Muthususbramanian R and Salivahanan S, "Basic Electrical,
Electronics and Computer Engineering" Tata McGraw Hill, 1999.
PHYSICS LABORATORY L P T C
PHY 183
Course Outcomes:
CO1: Learn the practical understanding of the mechanical properties such as modulus,
moment of inertia, gravitational force, stress, strain, etc
CO2: Understand and apply the optical phenomena like diffraction, interference, etc.
CO3: Understand the thermal conductivity and also thermal behavior of the specimen
CO4: Acquire practical skill to analyze the fluid state mechanism
CO5: Find thickness of very very thin objects
CO6: Learn the knowledge of generating ultrasonic waves and finding the velocity of it in
Liquid.
CO7: Determine the bandgap of semiconductor.

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List of Experiments:
1. To determine the acceleration due to gravity using Compound Pendulum

2. To determine the Rigidity Modulus of wire using Torsional Pendulum
3. To find thickness of the given two glass plates using single optic lever.
4. To determine the thermal conductivity of a bad conductor
5. To determine the refractive index of the material of the prism.
6. To find the number of rulings per cm length of the given transmission grating.
7. To determine the particle Size Using Laser
8. To determine the coefficient of viscosity of the liquid by Poiseuille•s method
9. To determine the young•s modulus of given material using Uniform Bending
10. To Determine the thickness of a given material using Air wedge method
11. To determine the focal length of a biconvex lens using Newton•s Rings method
12. To determine the velocity of ultrasonic waves in the given medium using ultrasonic
Interferometer.
L T P C
CSE 181 PROGRAMMING LANGUAGE LABORATORY
0 0 3 1
Course Outcomes:
CO1: Understand problem analysis, algorithm design, and program implementation
CO2: Write modular, efficient and readable C programs
CO3: Design modular programs with structured programming constructs
CO4: Formulate problems and implement algorithms in C and work in a team to develop
projects

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APPLICATION PACKAGES
1. Word Processing
2. Spreadsheet
3. Powerpoint
4. Database Management
C PROGRAMMING
1. Basics
2. Operators and Expressions
3. I/O formatting
4. Control Statements
ARRAYS AND FUNCTIONS

1. Arrays
2. String Manipulation
3. Functions
POINTERS, STRUCTURES AND FILES

1. Pointers
2. Structures and Unions
3. File Handling
UNIX PROGRAMMING
1. Basic Unix Commands

2. Basic Shell Programming
SEMESTER II
ENGLISH FOR TECHNICAL COMMUNICATION II L T P C

HSS102
Course Outcomes:
CO1: Identify the errors in sentence structures
CO2: Construct grammatically correct sentences
CO3: Frame conversations
CO4: Effectively construct utterances for a Dialogue
CO5: Prepare various components of official communication like Memos, Circulars, Notices
and Agendas
CO6: Recall Mechanics of Manuscript Preparation
CO7: Write reviews of a text, that the students read or a movie that they watch.

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GRAMMAR AND VOCABULARY

Grammar and Vocabulary - Introduction to grammatical models - Proper use of tenses,
concord, voice, articles, punctuation, and modal auxiliaries.
RECEPTION SKILLS
Listening and Language Development - Improving listening skills - comprehension practice -
Comprehend classroom lectures, simple technically oriented passages - Listening to news
bulletins, pre-recorded talks, different speech styles, comprehending the essential meaning -
Physical and psychological barriers to listening - Steps to overcome the barriers - Practice in
note-taking while listening.
SPEAKING TECHNIQUES
Speaking practice - Improving conversing skills - Improving self-expression - Developing
confidence and fluency in oral communication - Physical and psychological barriers to
speaking - Steps to overcome the barriers - Formal and public speaking practice -
Extemporary talk practice - Speech process - fluency and accuracy in speech - Developing
persuasive speaking skills - Conversation in a given milieu, social and cultural surroundings -
Practice in giving small talks on local topics for a minute or two - Goal oriented group
discussion - Participating in seminars - Independent and effective communication.
READING STRATEGIES
Reading comprehension - Vocabulary extension methods - Speed reading practice - technical
and non-technical materials - Practice in various reading techniques - skimming, scanning,
eye reading - Looking for specific information - Comprehending the given passages,
technical information.
WRITTEN COMMUNICATION
Basic grammatical structures - Alphabet of other languages - Paragraph writing - Expressing
the idea in writing - Avoiding and correcting common errors - Effective writing techniques -
brevity, clarity, objectivity and simplicity - Discourse writing - definition, description,
instruction - Note-making - Proof reading - Mechanics of writing - Writing formal, informal
letters, Technical reports - Reference skills - using dictionary better.
TEXT BOOKS
1. Rizvi M Ashraf, Effective Technical Communication, Tata McGraw-Hill, 2005.
2. Rutherfoord Andrea J, Basic Communication Skills for Technology, Pearson
Education, 2002.
REFERENCES
1. Deborah C Andrews, Margaret D Bickle, Technical Writing - Principles and Forms,
Macmillan, 1978.
2. Manivannan G, English for Engineers - A Book on Scientific and Technical Writing,
Govi Publications, 2005.
3. Sarah Freeman, Written Communication in English, Orient Longman, 2000.
4. Thomson A J & AV Martinet, A Practical English Grammar, OUP, 4th Edition, 1986.
5. Tom Hutchinson, Alan Waters, English for Specific Purpose, Cambridge University
Press, 1987.
MATHEMATICS II L T P C
MAT104
Course Outcomes:
CO1 : Explain the concept of double integral and triple integral.
CO2 : Explain the concept of Gradient, divergence and curl.
CO3 : Explain the concept of line, volume and surface integrals.
CO4 : Construct conformal mappings between regions.
CO5 : Evaluate certain real integrals using residue theorem.
CO6: Apply differential equations for Physical problems.

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SEQUENCES AND SERIES

Convergence and divergence of infinite series ‚ series of positive terms ‚ comparison,
D•Alembert•s ratio, Raabe•s and Cauchy•s root tests ‚ Convergence of alternating series ‚
Leibnitz•s test ( proof of theorems and tests not included) ‚ elementary notions of absolute
and conditional convergence - Power series ‚ Taylor•s theorem(one variable)
ANALYTIC FUNCTION AND CONFORMAL MAPPING

Function of a complex variable ‚ Analytic function ‚ Necessary conditions ‚ Cauchy ‚
Riemann equations ‚ Sufficient conditions (excluding proof) ‚ Properties of analytic function
‚ Harmonic conjugate ‚ Construction of Analytic functions - Conformal mapping - w = z+a,
az, 1/z, e z , sin z, cos z and bilinear transformation ‚ fixed points ‚ cross ratio
COMPLEX INTEGRATION
Statement and application of Cauchy•s integral theorem and integral formula ‚ Taylor and
Laurent expansions ‚ Isolated singularities ‚ Residues - Cauchy•s residue theorem - Contour
integration over unit circle and semicircular contours (excluding poles on boundaries)-
evaluation of real integrals using contour integration
MULTIPLE INTEGRALS
Review of Riemann integrals - Double integration ‚ Cartesian and polar coordinates ‚
change of order of integration ‚ change of variable between Cartesian and polar ‚ Area as
double integral ‚ Triple integration in Cartesian, cylindrical and spherical polar coordinates ‚
volume as triple integral
VECTOR CALCULUS
Gradient, Divergence and Curl ‚ Directional derivative ‚ Irrotational and solenoidal vector
fields ‚ Vector integration ‚ Green•s theorem in a plane, Gauss divergence theorem and
Stoke•s theorem (excluding proof) ‚ Simple applications
TEXT BOOKS
1. Kreyszig, E, Advanced Engineering Mathematics, John Wiley and Sons (Asia)
Limited, Singapore, 8th Edition, 2001
Volume II, Scitech Publications (India) Pvt. Ltd., Chennai, 1st Edition., Reprint 2000,
1999
REFERENCES
1. Grewal , B.S., Grewal, J.S., Higher Engineering Mathematics, Khanna Publishers,
New Delhi, 37th Edition., 5th Reprint 2004, 2003
2. Venkataraman, M. K., Engineering Mathematics First Year, The National Publishing
Company, Chennai, 2nd Edition., Reprint 2001, 2000
3. Venkataraman, M. K., Engineering Mathematics ‚ III A, The National Publishing

Company, Chennai, 11th Edition., Reprint 2002, 1998
ENGINEERING PHYSICS II L T P C
PHY 132
(common to all branches) 3 0 0 3
Course Outcomes:
CO1: Understand the free electron theories, formation of energy bands, energy distribution
and also the electron behavior in solids
CO2: Know and understand the cooper pair electron behavior, applications of
superconducting materials in developing technologies.
CO3: Learn the importance of semiconducting materials in engineering fields by projecting
the view of energy bands.
CO4: Gain the knowledge about various kinds of magnetic materials, their properties and
applications in advanced technologies.
CO5: Gain the knowledge about dielectric materials, their properties and significant
applications in advanced technologies.
CO6: Learn how to prepare some new materials like metallic glasses, nano-materials, shape
memory alloys, nonlinear materials to improve the technology.
CO7: Adapt to new developments of materials in science and technology by characterizing
with sophisticated instruments.

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CONDUCTING MATERIALS
Conduction in metals ‚ mobility and conductivity ‚ classical free electron theory - electrical
conductivity ‚ thermal conductivity ‚ Wiedemann-Franz law ‚ Lorentz number ‚ drawbacks
of classical theory.
SEMICONDUCTING AND SUPERCONDUCTING MATERIALS

Introduction semi conducting materials ‚ types of semiconducting materials ‚ carrier
concentration ‚ Hall effect ‚ determination of Hall coefficient ‚ superconducting phenomena
‚ properties of superconductors ‚ Type I and Type II superconductors ‚ high Tc
superconductors ‚ application of super conductors.
MAGNETIC MATERIALS
Classical theory of magnetism ‚ quantum theory of paramagnetism ‚ ferromagnetism ‚
ferrites ‚ applications of magnetic materials.
DIELECTRIC MATERIALS AND OPTICAL MATERIALS

Electronic, ionic, orientational and space charge polarization ‚ internal field and deduction of
Clausius-Mosotti relation ‚ properties of dielectric materials ‚ classification of insulating
materials ‚ optical properties of semiconductor ‚ imperfection of crystals ‚ luminescence ‚
fluorescence ‚ phosphorescence ‚ light emitting diode (LED) ‚ liquid crystal displays
(LCD).
NEW ENGINEERING MATERIALS

Metallic glasses as transformer core material ‚ nanophase materials ‚ shape memory alloys ‚
Bio materials (metals & alloys, ceramics) ‚ non-linear materials ‚ second harmonic
generation ‚ optical mixing - optical phase Conjugation ‚ solitons and IC packaging
materials.
TEXT BOOKS
1. P.K. Palanisamy, ƒMaterial Science„, Scitech Publications (India), Pvt Ltd., Chennai,
2009.
2. Arumugam, M., Material Science, Anuradha Agencies, Kumbakonam, 3 rd Edition,
2003.
REFERENCES
1. Aswani K.G., A Text book of Material Science, S.Chand & Co., Ltd., New Delhi, 2nd
Edition, 2001.
2. Raghavan, V., Material Science and engineering, Prentice-Hall of India Pvt. Ltd.,
India.
3. William F.Smith, Foundations of Materials Science and Engineering, McGraw-Hill,
New York, 3rd Edition, 2003.
4. Wahab M.A., Solid State Physics, Narosa Publishing House, New Delhi, 1999.
5. Pillai S.O., Solid State Physics, New Age International Publication, New Delhi, 5th
edition, 2003.
6. Ali Omar.M, Elementary Solid State Physics, Pearson Education (Singapore) Pvt.
Ltd., Indian Branch, New Delhi, 2002.
7. Murthy V.S.R., Jena AK, Gupta K.P. and Murthy G.S., Structure and Properties of
Engineering Materials, Tata McGraw-Hill Publishing Company Ltd, New Delhi,
2003.
L T P C
CIV 101 BASIC CIVIL AND MECHANICAL ENGINEERING
4 0 0 4
Course Outcomes:
CO1: Describe the scientific terminologies related to construction and mechanical sciences.
CO2: Familiarize with different components, equipments and technical standards
CO3: Know the purpose, procedures, and the materials
CO4: Be aware of the uses and standards adopted in industries.
CO5: Understand the basic laws pertaining towards the subject.
CO6: Understand the procedures for construction of several structures.
CO7: Create working models or prototypes of the components.
CO8: Gain knowledge in surveying, their types and the equipments used.
CO9: Explain the principle, working and application of Engines and Power plants.
CO10: Understand and apply the concepts of manufacturing and the technology related.
CO11: Mention some of the applications of the manufacturing processes.

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CIVIL ENGINEERING
BUILDINGS
Characteristics of good building materials such as stones, bricks, plywood and ceramic tiles,
timber, cement, aggregates and concrete - Basic functions of buildings ‚ Major components
of buildings ‚ Foundations - Purpose of a foundation ‚ Bearing capacity of soils ‚ types of
foundations. Proper methods of construction of Brick masonry ‚ Stone masonry ‚ Hollow
Block masonry. Beams ‚ Lintels ‚ Columns ‚ Flooring ‚ Damp proof course ‚ surface
finishes ‚ Doors and windows ‚ Roofing.
TRANSPORTATION ENGINEERING
Principles and Classification of surveying, Chain surveying, Compass surveying and leveling
- Importance of roads ‚ Classification of Highways ‚ water bound macadam, bituminous and
cement concrete roads ‚ . Railways - Importance of railways ‚ Gauges ‚ Components of a
permanent way. Bridges - Components of Culverts ‚ Causeways, Slab Bridge, T-beam and
slab bridge, Suspension bridge
MECHANICAL ENGINEERING
BOILERS AND TURBINES

Boilers - boiler mountings and accessories ‚ Cochran boiler, Locomotive boiler, Babcock
and Wilcox boiler, fire and water tube boilers - Steam turbine - single stage impulse turbine,
Parson•s reaction turbine, difference between impulse and reaction turbines.
POWER PLANTS AND INTERNAL COMBUSTION (IC) ENGINE

Classification of power plants ‚ steam, nuclear, diesel and hydro power plants - Alternate
sources of energy - solar, wind, tidal, geothermal, ocean thermal energy conversion. ‚ IC
engine - components, working of four and two stroke petrol and diesel engines.
PRODUCTION TECHNOLOGY
Metal casting and forming process ‚ patterns, moulding, melting of cast iron, casting ‚
forging ‚ rolling ‚ extrusion ‚ drawing - Metal joining process - welding ‚ arc welding, gas
welding, brazing and soldering - Metal machining ‚ lathe, drilling machine, milling machine,
shaping machine, planing machine, introduction to Computer Numerical Control
machining.
TEXT BOOK
1. Shanmugam, G,, and Palanichamy, M.S., Basic Civil and Mechanical Engineering,
Tata McGraw Hill Publishing Co., New Delhi, 1996.
REFERENCES
1. Khanna, K., Justo C E G, Highway Engineering, Khanna Publishers, Roorkee, 2001
2. Arora S.P. and Bindra S.P., Building Construction, Planning Techniques and Method
of Construction, Dhanpat Rai and Sons, New Delhi, 1997.
3. Venugopal K., Basic Mechanical Engineering, Anuradha Publications,
4. Kumbakonam, 2000.
5. Shanmugam G., Basic Mechanical Engineering, Tata McGraw Hill Publishing
Co.,New Delhi, 2001.
ENVIRONMENTAL SCIENCES L T P C
CHY 102
(Common to all branches) 3 0 0 3
Course Outcomes:
CO1: Know the importance of environmental studies and methods of conservation of natural
resources.
CO2: Describe the structure and function of an ecosystem.
CO3: Identify the values and conservation of bio-diversity.
CO4: Explain the causes, effects and control measures of various types of pollutions.
CO5: Select the appropriate methods for waste management.
CO6: Get knowledge about various disaster management methods
CO7: Recall social issues and legal provision.

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NATURAL RESOURCES
Definitions ‚ Scope of Environmental Sciences - Forest Resource ‚ Food Resource ‚ Land
Resource ‚ Water ‚ Mineral resources - Utilization of Natural Resource, Impact on
Environment ‚ Conservation of Natural Resources
ECOSYSTEM AND BIODIVERSITY

Concept ‚ Structure and Function ‚ Energy Flow in Ecosystem ‚ Ecological Succession ‚
Food Chain ‚ Food Web, Ecological Pyramids ‚ Biodiversity, Definition, Values, Threats to
Biodiversity, Conservation of Biodiversity
ENVIRONMENTAL POLLUTION
Definition, Causes, Effects and Control Measures of Air, Water and Soil Pollution ‚ Thermal
and Nuclear Pollution
MANAGEMENT OF ENVIRONMENTAL POLLUTION

Solid Waste Management ‚ Treatment Methods adopted for Municipal Sewage and
Industrial Effluent ‚ Hazardous and Biomedical Waste Management
TOOLS FOR ENVIRONMENTAL MANAGEMENT

Environment Impact Assessment ‚ Precautionary and Polluter Pay Principle - Constitutional
Provision ‚ (Air, Water and Forest) - Waste Minimization Techniques, Cleaner Technology
Options, Bioremediation
TEXT BOOK
1. Dhameja, S.K., Environmental engineering and Management, S. K. Kataria & Sons,
New Delhi, 1st Edition, 2004
REFERENCES
1. Bharucha Erach, The Biodiversity of India, Mapin Publishing Pvt. Ltd., Ahmedabad,
1st Edition, 2001.
2. Miller, T.G. Jr., Environmental Science, Wadsworth Publishing Co. USA, 2nd
Edition, 2004
3. Trivedi, R.K., Handbook of Environmental Laws, Rules, Guidelines, Compliances
and Standards, Vol. I and II, Enviro Media., New Delhi, 2nd Edition, 2004
4. Masters, G. M., Introduction to Environmental Engineering & Science, Prentice Hall,
New Delhi, 2nd Edition, 1997
5. Henry, J. G., and Heike, G. W., Environmental Science & Engineering, Prentice Hall
International Inc., New Jersey, 1st Edition, 2005
ENGINEERING DRAWING L T P C
MEC101
Course Outcomes:
CO1: Describe the scientific and empirical foundations for engineering design.
CO2: Familiarize with different drawing equipments and technical standards
CO3: Know the purpose, procedures, materials and conventional symbols used
CO4: Be aware of the uses of standard and nominal sizes in industries.
CO5: Understand the basic geometrical relationships; parallelism, perpendicularity,
angularity, co-linearity and concentricity.
CO6: Understand the procedures for construction of geometric figures.
CO7: Create and read an engineering drawing using standard views
CO8: Know the principles projection and distinguish the types of projection
CO9: Convert pictorial (3-D) drawings to orthographic (2-D) drawings and vice versa
CO10: Explain the principle and application of sectioning
CO11: Understand and apply the concepts of development of surfaces
CO12: Mention some of the applications of technical drawings

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INTRODUCTION
Importance of graphics ‚ use of drafting instruments ‚ BIS conventions and specifications ‚
size, layout and folding of drawing sheets ‚ lettering dimensioning and scales - orthographic
principles ‚ missing view - free hand sketching in first angle projection from pictorial views.
PROJECTION OF POINTS, STRAIGHT LINES AND PLANES

Projection of points, located in all quadrants - projection of straight lines located in the first
quadrant, determination of true lengths and true inclinations, location of traces - projection of
polygonal surface and circular lamina located in first quadrant inclined to one or both
reference planes.
PROJECTION OF SOLIDS AND SECTION OF SOLIDS

Projection of solids like prisms, pyramids, cylinder and cone when the axis is inclined to one
reference plane by change of position method ‚ types of section ‚ full section and half
section -conventional section lines - section of simple solids like prisms, pyramids, cylinder
and cone in vertical position by cutting planes inclined to any one of the reference planes,
obtaining true shape of section
DEVELOPMENT OF SURFACES
Development of lateral surfaces of simple and truncated solids ‚ prisms, pyramids, cylinders
and cones - development of lateral surfaces of combined solids.
ISOMETRIC AND PERSPECTIVE PROJECTION

Principles of isometric projection ‚ isometric view and projections of simple solids, truncated
prisms, pyramids, cylinders and cones - Orthographic to isometric view ‚ Introduction to
perspective projection.
TEXT BOOK
1. Basant Aggarwal and C. Aggarwal, Engineering Drawing, Tata McGraw-Hill
publishing company,New Delhi , 2008
REFERENCES
1. Shah, M.B., and Rana, B.C., Engineering Drawing, Pearson Education, New Delhi,
2005.
2. Nataraajan, K.V., A text book of Engineering Graphics, Dhanalakshmi Publishers,
Chennai, 2006.
3. Bhatt, N.D., Engineering Drawing, Charotar publishing House, New Delhi, 46th
Edition, 2003.
4. Luzadder and Duff, Fundamentals of Engineering Drawing, Prentice Hall of India Pvt
Ltd, New Delhi, XI Edition, 2001.
5. Venugopal, K., Engineering Graphics, New Age International (P) Limited, 2002.
L T P C
BIT103 CELL BIOLOGY AND GENETICS
3 0 0 3
Course Outcomes:
CO1: Distinguish prokaryotic cell from eukaryotic cell and describe the structure and
function of different parts of a eukaryotic cell
CO2: Explain the mitosis and meiosis cell division and the consequences
CO3: Explain different types of microscopes and their main uses
CO4: Appreciate the discovery of Mendelian laws
CO5: Describe human chromosome and basis of genetic diseases

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STRUCTURE AND FUNCTION OF CELL ORGANELLES

History of cell - Development of cell theory - General organization of prokaryotic and
eukaryotic cell - comparison - Organization of cell membrane ‚ Cell permeability ‚
Differentiations of the cell membrane -Intercellular communications and Gap junctions ‚
Cytoskeleton and cell motility - Microtubules, microfilaments and intermediate filaments ‚
Endoplasmic reticulum and protein segregation ‚ Golgi complex and cell secretion -
Lysosomes, endocytosis, coated vesicles, endosomes and peroxisomes ‚ Mitochondria and
Oxidative Phosphorylation ‚ Chloroplast and Photosynthesis- ribosomes- nucleolus‚
chromatin and chromosomes.
CELL CYCLE AND CELL DIVISION

The cell cycle ‚ Regulation - Role of cyclins and Cdks, Cell cycle check points, ‚ General
description and different stages of mitosis and meiosis ‚ Functional role of mitotic apparatus
‚ Consequences of meiosis and types of meiosis ‚ difference between mitosis and meiosis.
TYPES OF MICROSCOPES AND APPLICATIONS

Microscopy ‚ Simple and Compound Microscope, Different types of light microscope and
their applications, Confocal microscope, Electron microscopes- Transmission Electron
Microscope and Scanning Eelectron Microscope,‚ - Sample preparation for light microscopy
and electron microscopy.
CONCEPTS IN GENETICS
History ‚ Mendelian experiments with pea plants ‚ self pollination and cross pollination-
Mendelian Laws of inheritance: dominance and recessive, law of segregation and law of
independent assortment -Linkage and Crossing over- Linkage mapping ‚ chromosome
mapping- Extra chromosomal inheritance- population and evolutionary genetics
HUMAN GENETICS
The human chromosome, chromosome abnormalities - Genotype and allelic frequencies-
Inborn-errors of metabolism - polygenic and multifactorial inheritance ‚ Sex determination -
Role of Y chromosome ‚ sex-linked inheritance, sex chromosome anomalies - Mutation -
Gene mutation - molecular basis of mutation - Molecular basis of genetic diseases
TEXT BOOKS
1. De Robertis, E.D.P. and De Robertis, E.M.F., Cell and Molecular Biology, Lippincott
Williams & Wilkins, Philadelphia, USA, 8th Edition, 2010.
2. Gardner, E.J., Simmons, M.J. Snustad, D.P. Principles of GeneticsWiley-India Ltd,
New Delhi , 8th Edition, 2008.
3. Strachan, T., and Read A.P. Human Molecular Genetics, Garland Publishing, 3rd
Edition, 2004.
L T P C
MEC181 WORK SHOP
0 0 3 1
Course Outcomes:
CO1: Make Joints and understand their uses in Wooden Products like Table, Frame, etc…
CO2: Perform metal joining with simple saw process
CO3: Make hollow channels, containers using Sheet metal development
CO4: Join Metal using Welding process (Knowledge only)
CO5: Gain knowledge in Casting and Molding of Metals
CO6: Perform various Machining Techniques like Drilling, Tapping, etc…

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CARPENTRY
Carpentry tools - practice in marking, sawing, planing and chiseling ‚ making simple joints:
lap joint, T-joint, dovetail joint, mortise and tenon joint.
FITTING
Fitting tools - practice in marking, filing, punching, hacksawing - fitting to size and drilling -
making of simple mating profiles: V, square, dovetail, half round joints.
SHEET METAL
Study of press, die and tools - sheet metal layout - development of lateral surfaces -simple
exercises: blanking, forming, bending and flanging.
DRILLING
Drilling and tapping in drilling machines
Demonstration on:
(i) Welding operations like butt joint and lap joints in Arc welding
(ii) Foundry operations like mould preparation for split pattern
(iii) Smithy operations like the production of hexagonal bolt
(iv) Preparation of plumbing line sketches ‚ basic pipe connections involving the fittings like
valves, taps, couplings, unions, reducers, elbows and other components used in household
fittings.
L T P C
CHY 182 CHEMISTRY LABORATORY
0 0 3 1
Course Outcomes:
CO1: Analyze the various water quality parameters.
CO2: Investigate the kinetics of a chemical reaction.
CO3: Determine the amount of fluoride and iron by spectrophotometric methods
CO4: Estimate the amount of acid and base by electrochemical methods.

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1. Estimation of hardness of water sample by EDTA method
2. Determination of alkalinity of given water sample
3. Determination of dissolved oxygen in a water sample
4. Determination of rate constant of a reaction (Ester hydrolysis)
5. Estimation of hydrochloric acid by pH titration
6. Estimation of chloride ion in a given water sample
7. Determination of sodium and potassium by flame photometry
8. Estimation of ferrous ion by potentiometric method
9. Estimation of iron by spectrophotometry using 1,10-phenanthroline
10. Determination of strength of mixture of acids using strong base by conductometric
titration
11. Estimation of fluoride ion by spectrophotometry
12. Conductometric titration of strong acid with strong base
SEMESTER III
MATHEMATICS III L T P C
MAT202 (Common to Biotechnology, Chemical Engineering, Civil
3 0 0 3
Engineering, CSE, EEE, ICE and Mechanical Engineering)
Course Outcomes:
CO1: Evaluate integrals and solve boundary value problems using Laplace transfoms
CO2: Solve standard type of first order partial differential equations and higher order partial
differential equations with constant coefficients.
CO3: Apply the concept of Fourier series to find the sum of certain series.
CO4: Solve difference equations using Z-transform.
CO5: Find Fourier, Sine and Cosine transforms of given functions.

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CO4 M M M
CO5 M M M
LAPLACE TRANSFORM
Definition of Laplace transform - Linearity property - condition for existence of Laplace
transform - First and second shifting properties - Laplace transform of derivatives and
integrals - Unit step functions - Diarc delta-function - Differentiation and integration of
transforms - Convolution theorem - Inversion - Periodic functions - Evaluation of
integrals by Laplace transform - Solution of boundary value problems
PARTIAL DIFFERENTIAL EQUATIONS

Formation of PDE - Solution of std types of first order PDE - Lagrange•s linear equation -
Linear PDE of second and higher order with constant coefficients
FOURIER SERIES
Dirichlet•s conditions - General Fourier series - odd and even functions - Half range sine and
cosine series - complex form of Fourier series - Parseval•s identity - Harmonic analysis
Z € TRANSFORM
Z-transform - elementary properties - Inverse Z-transform - Convolution theorem - formation
of difference equation - Solution of difference equation using Z-transform.
FOURIER TRANSFORM
Fourier Integral formula - Fourier Transform - Fourier sine and cosine transforms - Linearity,
Scaling, frequency shifting and time shifting properties - Self reciprocity of Fourier
Transform - Convolution theorem - Application to boundary value problems
TEXT BOOKS
1. Kreyszig, E., Advanced Engineering Mathematics, John Wiley and Sons (Asia)
Limited, Singapore, 8th Edition., 2001
Volume II, Scitech Publications (India) Pvt. Ltd., Chennai, 1st Edn., Reprint 2000,
1999
REFERENCES
1. Grewal, B.S., Grewal, J.S., Higher Engineering Mathematics, Khanna Publishers,
New Delhi, 37th Edition, 5th Reprint 2004, 2003
2. Venkataraman, M. K., Engineering Mathematics ‚ III A, The National Publishing
3. Venkataraman, M. K., Engineering Mathematics - III B, The National Publishing
L T P C
CHE 253 BIOPROCESS CALCULATIONS
3 1 0 4
Course Outcomes:
CO1: Analyze the data obtained from an experiment using various analytical and graphical
methods
CO2: Solve material balance of physical processes
CO3: Solve material balance of chemical processes
CO4: Solve energy balance of physical and chemical processes
CO5: Solve combined material balance and energy balance for selected bioprocesses

CO1 H M M
CO2 H M M
CO3 H M M
CO4 H M M
CO5 H M M
INTRODUCTION
Units and dimensions, Concept of mole and Molecule, Composition of mixtures of Solids,
liquids and gases - Composition of mixtures and solutions- Percentage by weight, mole and
Volume; normality, molarity, molality, and ppm, pH and pKa Buffer Calculations
MATERIAL BALANCE WITHOUT CHEMICAL REACTIONS:

Material balances in distillation, absorption, extraction, crystallization, psychrometry, drying,
mixing and evaporation operations, Material balances involving bypass, recycle and purge.
MATERIAL BALANCE INVOLVING CHEMICAL REACTIONS

Principles of stoichiometry, definitions of limiting and excess reactants, fractions and
percentage conversion, yield and percentage yield, Selectivity and related problems.
THERMOPHYSICS AND THERMOCHEMISTRY

Thermophysics: Heat-capacity of gases, empirical equations for heat capacities, mean-heat
capacities of gases, latent heats, calculation of enthalpy from thermo physical properties.
Thermochemistry: Standard heat of reaction, heat of formation, law of Hess, standard heat of
combustion, heats of formation calculated from heats of combustion, calculation of the
standard heat of reaction from heats of formation or combustion, effect of temperature on
heat of reaction, enthalpy changes in reactions with different temperatures, calculation of
theoretical flame temperature.
STOICHIOMETRY OF BIOPROCESS
Material balance: Material balance involved in bioprocess, Stoichiometry of growth and
product formation
Energy balance: Heat of reaction for process with biomass production, Thermodynamics of
microbial growth, Energy balance for cell culture.
TEXT BOOKS
1. Bhatt, B.I. and Vora, S.M., Stoichiometry, Tata McGraw-Hill Publishing Company,
New Delhi, 4th Edition, 2004.
2. Doran, P.M., Bioprocess Engineering Principles, Academic Press (An Imprint of
Elsevier), New Delhi, 2nd Edition, 2005
REFERENCES
1. Himmelblau, D.M. Basic Principles and Calculations in Chemical Engineering,
Prentice-Hall of India, New Delhi , 7th Edition, 2004.
2. Hougen, O.A., Watson, K.M., and Ragatz, R.A., Chemical Process Principles, Part-I,
CBS Publishers and Distributors, New Delhi, 2nd Edition, 1995.
L T P C
BIT 204 MICROBIOLOGY
3 0 0 3
Course Outcomes:
CO1: Describe diversity of microorganisms, bacterial cell structure and function, microbial
growth and metabolism, and the ways to control their growth by physical and chemical
means
CO2: Demonstrate the knowledge as to how microorganisms interact with their environment
and interaction between humans and microorganisms
CO3:Explain the cell structure and metabolism and basic genetic systems of bacteria,
bacteriophage and plasmids
CO4: Demonstrate practical skills in fundamental microbiological techniques.
CO5: Systematically apply the scientific method of investigation and hypothesis testing
including the development of theoretical and practical skills in the design and
execution of experiments as well as the development of oral and writing skills
necessary for the effective communication of experimental results

CO1 M H H
CO2 H H M H H
CO3 H H H H
CO4 H M H H
CO5 H H H M
MICROBIAL WORLD
Basic of Microbial existence, History of microbiology, Classification and Nomenclature of
microorganisms, Type cultures and culture collections, Principles of Light, Phase contrast
and Dark field Microscopy, Electron microscope - Staining Techniques - Gram•s and Acid-
fast staining, spore, capsular and flagellar staining ‚ Pure culture techniques.
STRUCTURE AND FUNCTION

Structure of the bacterial cell wall, appendages of bacteria - Pili and flagella, capsule, slime
and bacterial endospores, Over view of Viruses, Structure, classification and multiplication
of algae and fungi, Life history of yeast and bacteriophages.
BACTERIAL PHYSIOLOGY AND GENETICS

Nutritional requirements of bacteria, Cultivation of bacteria - Different media used for
bacterial culture, Bacterial growth curve - measurement, kinetics and generation time,
Bacterial growth under aerobic and anaerobic conditions, Utilization of energy for
biosynthesis of important molecules. Bacterial reproduction - binary fission -
Transformation, conjugation and transduction ‚ mutation and recombination
MEDICAL MICROBIOLOGY
Microorganisms as pathogens- common infectious diseases ‚ cholera, typhoid, tuberculosis,
HIV, rabies, pappiloma, hepatitis Antibiosis: assay, common antibiotics ‚ ampicillin and
kanamycin and their mode of action
MICROBIAL ECOLOGY
Effect of environmental factors on the growth of microorganisms, Control of microorganisms
- antibiotics and disinfectants, Plant associates microorganisms - Rhizosphere, phylloplane
micro flora. Mycorrhiza, air and water micro flora, Host-microbe Interaction, Microbes and
the environment
TEXT BOOK
1. Pelczar M. J., Chan E.C.S. and Krieg N.R., Microbiology, Tata McGraw Hill, New
Delhi, India, 5th Edition, 1998.
REFERENCES
1. Prescott.L. M., Harley J.P. and Klein B.A., Microbiology, Wm. C. Brown Publishers,
IOWA. USA, 5th Edition, 2003.
2. Tortora, G.J., Funke, B.R. and Case, C.L., Microbiology: An Introduction, Benjamin
Cummings, 10th Edition, 2009.
L T P C
BIT 209 MOLECULAR BIOLOGY
3 0 0 3
Course Outcomes:
CO1: Summarize DNA as a genetic materials, organization and packing of genes in
chromosomes of both prokaryotic and eukaryotic systems
CO2: Describe replication, repair and recombination of DNA, in both prokaryotic and
eukaryotic organism
CO3: Explain the structure and function of RNA polymerase and how they are involved in
transcription with cap formation, splicing and polyadenylation
CO4: Understand genetic code, types of ribosome, RNA and how they are involved in
translational machinery of an organism
CO5: Illustrate various molecular biological techniques such as Southern, Northern and
western blotting, PCR types, and next generation sequencing

CO1 H - - - - M - - - M - -
CO2 H - - - - M - M - H - -
CO3 H - - - - M - H - H - -
CO4 H - - - - M - H - H - -
CO5 H - - H M M M M M M - M
GENES, GENOMES AND DNA

DNA as the genetic material ‚ Organization of prokaryotic and eukaryotic genomes,
supercoiling, repetitive DNA - Levels of DNA packaging in Eukaryotes - Coding and non-
coding DNA
DNA REPLICATION
Prokaryotic and eukaryotic DNA replication - mechanisms of DNA replication- fidelity of
replication- enzymes and accessory proteins involved in DNA replication - replication
inhibitors - DNA Repair and recombination; Gene mutations - types of mutations -
Suppression, Transposable genetic elements
TRANSCRIPTION
RNA Polymerase - holoenzyme and apoenzyme - sigma factors ‚ Initiation, elongation,
termination mechanism of eukaryotic and prokaryotic transcription - Types of RNA
polymerases - Promoter of RNA polymerase II - Enhancers - General and inducible
transcription factors - modifications of RNA - 5• cap formation ‚ polyadenylation - splicing
of nuclear pre-mRNA, mRNA stability ‚ Run-on Run-off mechanism - transcription
inhibitors
TRANSLATION
Genetic code ‚ characteristics -deciphering the code - protein biosynthesis- prokaryotic and
eukaryotic translation, the translational machinery, mechanism of initiation, elongation and
termination - regulation of expression in eukaryotes - regulation of translation by 3• and 5•
UTR motifs ‚ translation inhibitors
TECHNINIQUES IN MOLECULAR BIOLOGY

Blotting and hybridization, DNA sequencing - Sangers method - DNA fingerprinting; PCR,
RT-PCR and qPCR, transcriptome analysis ‚ Next generation sequencing
TEXT BOOKS
1. Allison, L.A, Fundamental Molecular Biology , Blackwell publishing group,
Malden, MA, USA, 1st Edition, 2007.
2. Friefelder. D., Molecular Biology, McGraw-Hill Companies, New York, USA, 5th
Edition, 2013.
REFERENCE
1. Clark, D.P. and Pazdernik, N.J., Molecular Biology, Elsevier Academic Press , 2nd
Edition, 2013
ANALYTICAL TECHNIQUES IN L T P C
BIT214
BIOTECHNOLOGY 3 0 0 3
Course Outcomes:
CO1: Describe the working principles of pH meter and estimation of macromolecules
CO2: Explain the principles and instrumentation of spectroscopy.
CO3: Describe the principles of centrifugation methods
CO4: Classify separation methods
CO5: Understand the principles of chromatography

CO1 H M M
CO2 H M M
CO3 H M M
CO4 H M M
CO5 H M M
BASIC TECHNIQUES
Measurement of pH: pH Indicators, pH Meter, titration of acids, preparation of buffers.
Estimation of macromolecules ‚ carbohydrates, proteins, lipids
COLORIMETRY AND SPECTROSCOPY

Properties of electromagnetic radiation - interaction with matter; Beer-Lambert•s Law -
differences between spectrophotometer and colorimeter - Visible light spectroscopy:
Principle, instrumentation and applications. Ultraviolet spectroscopy - Atomic absorption
spectroscopy, Thermogravimetric analysis, Spectrofluorimetry, FTIR, NMR and mass
spectrometry.
CENTRIFUGATION
Basic principles; Types of centrifuge - microfuge, and high-speed and ultracentrifuge;
Preparative centrifugation; Differential and density gradient centrifugation; applications
(Isolation of cell components); analytical centrifugation; determination of molecular weight
by sedimentation velocity and sedimentation equilibrium methods
ELECTROPHORESIS
Theory and applications of electrophoresis; agarose and polyacrylamide Disc- and slab-gel
electrophoresis; Gradient electrophoresis - Capillary electrophoresis; 2D Electrophoresis -
Isoelectric focusing, pulse-field gel electrophoresis
CHROMATOGRAPHIC METHODS OF SEPARATION

Introduction to chromatography - models, ideal separation, retention parameters Van -
Deemter equation ‚ TLC, HP-TLC and Column chromatography ‚ chromatographic
separation of proteins ‚ ion-exchange, affinity, hydrophobic interaction and size-exclusion
chromatography; gas chromatography (GLC) and high-performance Liquid Chromatography
(HPLC)
TEXT BOOK
1. Friefelder. D., Physical Biochemistry, Application to Biochemistry and Molecular
Biology, W.H. Freemen and Company, San Francisco, 2nd edition, 1982.
REFERENCES
1. Griffiths, O. M., Techniques of Preparative, Zonal and Continuous Flow
Ultracentrifugation, 1983.
2. William, B.L. and Wilson, K., A Biologist•s Guide to Principles and Techniques,
1986.
L T P C
BIT 211 PRINCIPLES OF BIOCHEMISTRY
3 0 0 3
Course Outcomes:
CO1: Describe the role of biomolecules and their applications
CO2: Calculate the pH of required buffers
CO3: Classify vitamins and their deficiency symptoms
CO4: Compare the structures of amino acids and lipids
CO5: Explain the role of hydrogen bonds in DNA structure

CO1 H H M
CO2 H M H M
CO3 M M M H
CO4 H M M
CO5 M M M
BASIC PRINCIPLES AND CHEMISTRY OF LIFE

Solution of non electrolytes & Electrolytes: Concentration expressions, ideal solutions,
colligative properties. Arrhenius theory, strong electrolytes - Ionic equilibrium,
Electromotive force. Bonds: ionic bonding, Ion-dipole. Covalent, H-bonds, Vander Waal•s
interaction, Hydrophobic and hydrophilic interactions. Water as a biological solvent and its
role in biological processes; Calculation of pH, Henderson-Hasselbalch equation, concept of
buffer, strength of buffer, range of buffer, important biological buffers; isotopes and their
use in biology.
CARBOHYDRATES AND VITAMINS

Classification, structure and reactions of monosaccharides, and disaccharides,
Polysaccharides ‚ structural polysaccharides and storage polysaccharides;
Homopolysaccharide and Hetero-polysaccharides; vitamins ‚ Fat soluble and water soluble
vitamins; Classification, structures and physiological functions
AMINOACIDS AND PROTEINS

Structure and characteristics of amino acids - Peptide bond stability and formation-Structural
organization of proteins - primary, secondary, tertiary, quaternary and subunit structure of
protein - Conformation of proteins globular and fibrous proteins
LIPIDS
Structure, and properties classification of lipids, fatty acids, waxes, phospholipids,
cerebrosides, lipoproteins and gangliosides- Prostaglandins - prostacyclins, leukotrienes,
thromboxanes and physiological implications- Steroids and bile acids.
NUCLEIC ACIDS
Structure of purines, pyrimidine, nucleosides and nucleotides - stability and formation of
phosphodiester bond - Watson and Crick model of DNA; calculation of †Tm• for
oligonuclotides; Types of RNA- Structure of tRNA
TEXT BOOKS
1. Nelson.D.L, Cox. M. M., Lehninger•s Principle of Biochemistry. 5th ed. Freeman,
2008.
2. Murray. R.K., Granner, D.K., Mayes. P. A. and Rodwell, V.W., Harper•s
Biochemistry, McGraw Hill, 27th Edition, 2006.
REFERENCES
1. Berg. J.M., Tymoczko.J.L., Stryer, L., Biochemistry, Freeman, 6th edition, 2006.
2. Zubay , Biochemistry, William C. Brown Publication, 4th Edition, 1998.
3. Voet, D., Voet, G., Biochemistry, John Wiley and Sons, Singapore, 3rdEdition, 2001.
L T P C
BIT 281 BIOCHEMISTRY LABORATORY
0 0 3 2
Course Outcomes:
CO1: Explain the concept of pH
CO2: Perform calibration of pH meter and colorimeter.
CO3: Prepare the required buffers
CO4: Identify unknown amino acids from titration curves
CO5: Analyze carbohydrates, lipids and amino acids qualitatively and quantitatively

CO1 H M
CO2 H M
CO3 H M
CO4 M H
CO5 M H M
1. Preparation of buffers (acidic, basic, neutral, biological)
2. Titration curves for amino acid, determination of pKa and pI
3. Qualitative analysis of lipids, carbohydrates and amino acids
4. Determination of aminoacids by ninhydrin method and Sorensen formal
titration(glycine)
5. Estimation of protein (Biuret and Lowry Method)
6. Estimation of total sugars by Anthrone method
7. Estimation of total sugars by DNS method
8. Estimation of aldose and ketose sugars
9. Determination of acid value, saponification value and iodine number of oils
10. Determination of acid value, saponification value and iodine number of fats
L T P C
BIT 283 MICROBIOLOGY LABORATORY
0 0 3 2
Course Outcomes:
CO 1: Prepare nutrient agar media and pour in Petri plates.
CO 2: Stain and differentiate between gram positive and gram negative bacteria.
CO 3: Perform serial dilution and plating
CO4: Perform single colony streaking
CO5: Draw and interpret a growth curve of bacterial culture

CO 1 M M H H M
CO 2 H M M H H M M M
CO 3 H H M M
CO4 H M M M H M
CO5 H H H M M
1. Sterilization techniques and handling method of glass wares and instruments
2. Microscopy and micrometry
3. Staining techniques - Simple, Gram•s, Capsule and Endospore staining
4. Motility test by hanging drop technique
5. Enumeration of Soil Microorganisms - serial dilution and plating
6. Pure culture technique
7. Biochemical tests - IMViC Test, catalase, oxidase, starch hydrolysis
8. Antimicrobial assay - (Kirby Bauer method)
9. Effect of disinfectants ‚ phenol coefficient.
10. MPN technique.
11. Phage titration.
12. Phage lysis of liquid culture
13. Examination of quality of milk ‚ Methylene blue test
14. Storage of microorganisms.
15. 16s rDNA sequencing.
CELL AND MOLECULAR BIOLOGY L T P C

BIT286
LABORATORY 0 0 3 2
Course Outcomes:
CO1: Observe sub-cellular organelles under the microscope
CO2: Visualize DNA bands in agarose gels
CO3: Isolate plasmid DNA from bacteria
CO4: Isolate genomic DNA from bacteria and plant
CO5: Prepare competent cells for transformation

CO1 M H
CO2 M H
CO3 M H
CO4 M H
CO5 M H H
1. Cell fractionation - isolation of sub cellular organelles such as mitochondria,
chlororplast etc.
2. Cell division in Onion root tip
3. Isolation of protoplast from plant leaves
4. Polytene Chromosomes of Dipterans
5. Estimation of nucleic acids
6. Isolation of plasmid DNA from bacteria
7. Agarose gel electrophoresis
8. Preparation of competent cells and transformation
9. Isolation of genomic DNA from bacteria
10. Isolation of genomic DNA from plant and animal cell
11. Restriction enzyme digestion
12. Mutagenesis ‚ UV and chemical
13. Southern and Northern blotting
14. Separation of Proteins - SDS-PAGE
SEMESTER IV
L T P C
CHE 252 UNIT OPERATIONS
3 1 0 4
Course Outcomes:
CO1: Explain the phenomena of fluid statics and dynamics and their applications
CO2: Describe the principles of mixing & agitation and its applications
CO3: Explain the concepts of filtration & sedimentation and its applications
CO4: Describe the mechanism of heat transfer
CO5: Discuss heat exchangers used in process industries

CO1 H H M M
CO2 H H M M
CO3 H H M M
CO4 H H M
CO5 H H M M
FUNDAMENTALS OF FLUID MECHANICS AND APPLICATIONS

Properties of fluids - Fluid statics, pressure measurement, forces on submerged bodies -
Equation of continuity and motion - Bernoulli•s equation and its applications - Newtonian
and non-Newtonian fluids - Laminar and turbulent flows - Pressure drop calculation and
friction factor - Principles and operation of variable head meter and variable area meter -
Fluid moving machineries such as pumps, blowers, compressors, vacuum system -
Application and selection of valves
AGITATION AND MIXING

Dimensional analysis - Power for agitation, Agitation of liquids - Gas-liquid systems -Gas-
solid suspensions - Agitator scale up
FILTRATION & SEDIMENTATION

Constant pressure and constant volume batch filtration, continuous filtration - Industrial
filters - batch sedimentation test - Centrifugation
MECHANISM OF HEAT TRANSFER

Steady state conduction - Combined resistances - Unsteady state conduction - Combined
conduction and convection ‚ Lumped heat capacity - Extended surfaces - Forced and natural
convection ‚ Correlations for convective heat transfer coefficient for convection in flow over
surfaces and through pipes - Boiling and condensation
HEAT EXCHANGERS AND EVAPORATORS

Heat exchangers - Overall heat transfer coefficients - Design of heat exchangers, NTU
concept ‚ Evaporators - single and multiple effects - Mass and enthalpy balances
TEXT BOOKS
1. McCabe, W. L., Smith, J. C., and Harriott, P., Unit Operations of Chemical
Engineering, McGraw Hill, New York, 7th Edition, 2005
2. Holman, J. P., Heat Transfer, Mc Graw Hill, New York, 9th Edition, 2002.
3. Geankoplis, C.J., Transport Processes and Unit Operations, Prentice Hall of India,
New Delhi, 3rd Edition, 2002
REFERENCES
1. David, M. Himmelblau, Basic Principles and Calculations in Chemical Engineering,
Prentice-Hall of India, New Delhi, 7th Edition, 2004.
2. Coulson, J.M., Richardson, J.F, Backhurst J.R. and Harker J.M., Coulson and
Richardson•s Chemical Engineering, Volume I, Butter worth Heinemann, Oxford,
New York, 5th Edition, 2002.
L T P C
BIT203 BIOENERGETICS AND METABOLISM
3 1 0 4
Course Outcomes:
CO1: Describe the fundamental concepts of biochemistry and their implications for biology.
CO2: Write the pathways involved in the synthesis and regulation of macromolecules
CO3: Explain the role of enzymes in biosynthesis.
CO4: Discuss the metabolic disorders of nucleic acids.
CO5: Summarize the cell metabolism and various reactions

CO1 H H M M
CO2 H H M M
CO3 H M M
CO4 M M
CO5 H M M
BIOENERGETICS
Laws of thermodynamics, entropy, enthalpy, free energy and equilibrium constant, Gibbs
free energy equation, Biological oxidation and reduction, Redox potential and phosphate
potential - Thermodynamic considerations. High energy compounds - Requirements of ATP
for synthesis and degradation cycle. - Photosynthesis - Photo systems, light and dark
reactions, C3 and C4 pathways
CARBOHYDRATE METABOLISM
Glycolysis, Tricarboxylic acid cycle (TCA),Glyoxylate cycle - Pentose phosphate pathway
(HMP) gluconeogenesis and Glycogen metabolism, oxidative phosphorylation, Electron
transport chain (ETC), Chemiosmotic theory- oxidative phosphorylation, inhibitors and un-
couplers of electron transport chain and function of ATPase (bacterial and mitochondrial),
Shuttle systems.
PROTEIN METABOLISM
Urea cycle, Biosynthetic and degradative pathway of amino acids - Protein synthesis and
degradation - Different levels of regulation -Allosteric regulation and feedback regulation
proteolytic processing.
LIPID METABOLISM
Fatty acids metabolism - Biosynthesis of fatty acids - -oxidation pathway - Ketone bodies -
Control of metabolism - Biosynthesis and degradation of cholesterol.
NUCLEIC ACID METABOLISM

Biosynthesis of purine - Nucleotides (adenine, guanine) - Biosynthesis of pyrimidine -
Nucleotides (cytosine, thymine, and uracil) - Catabolism of adenine, guanine, cytosine,
thymine, and uracil - Metabolic disorders associated with purine metabolism - Metabolic
disorders associated with pyrimidine metabolism
TEXT BOOKS
1. Voet and Voet. Biochemistry. John Wiley and Sons, Singapore, 4th Edition, 2010.
2. Murray. R.K, Granner.D.K, Mayes. P. A, Rodwell. V. W. Harper s Biochemistry.
McGraw Hill, 27th Edition, 2006.
REFERENCES
1. Stryer, L.Berg., J.M.Tymoezko., J.L., Biochemistry, W.H. Freeman Co., New York,
5th Edition, 2002.
2. Zubay, G., Biochemistry, McGraw Hill Publishers, New Delhi, 4th Edition, 1999.
3. Lehninger•s, A.L. Nelson., D.L., Cox, M.M., Principles of Biochemistry, Worth
Publishers, London,4th Edition, 2000.
L T P C
BIT205 INDUSTRIAL BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1:Illustrate the screening procedures of microbes of industrial importance
CO2:Explain the medium requirements for fermentation processes
CO3:Compare various types of fermentation processes
CO4:Sketch and describe the production of industrially important products
CO5:Discuss the production of microbial enzymes, vaccines and microbial transformations

CO1 H M M H
CO2 M H H H
CO3 H M H M M
CO4 H H H M H
CO5 H H M H H M H
INTRODUCTION TO INDUSTRIAL BIOPROCESS

Introduction to fermentation process - definition, scope, history, microorganisms and
industrial products - Screening for microbes of industrial importance - Isolation and
preservation of industrial microorganisms - Primary screening (screening for amylase,
organic acid, antibiotic, amino acid and vitamin producing microorganisms) and secondary
screening - Further evaluation of primary isolates - Detection and assay of fermentation
products - Physico chemical methods and biological assays - Role of a bioprocess engineer
in the biotechnology industry - Outline of the various unit operations involved in an
integrated bioprocess - Process flow sheeting
STRAIN IMPROVEMENT AND MEDIA PREPARATION

Environmental factors and genetic factors for improvement ‚ Methods of strain improvement
‚ mutation and rDNA technology, Inoculum media and inoculum preparation - Medium
requirements for fermentation process. Examples of simple and complex media, raw
materials, saccharides, starchy and cellulosic materials
FERMENTATION PROCESS
Types of fermentation processes - Solid state, surface and submerged fermentations - batch,
fed batch, continuous fermentations - Direct-dual or multiple fermentations - Scale up of
fermentations
PRODUCTION OF PRIMARY AND SECONDARY METABOLITES

Fermentative production of industrial alcohol, beer - Principles of wine making.
Fermentative production of citric acid, vitamin B12, glutamic acid. Antibiotics - commercial
production of benzyl penicillin and tetracycline•s.
PRODUCTION OF MODERN BIOTECHNOLOGICAL PRODUCTS

Immobilization - Production and application of microbial enzymes - Amylases, lipases and
proteases enzymes in biotransformation - Steroid transformations - Microbial biopesticides
and biofertilizers - Principles of vaccine production and types of vaccines - Microbial
products from genetically modified (cloned) organisms.
TEXT BOOKS
1. Wulf Cruger and Anneliese Cruger., Biotechnology, (A text book of industrial
Microbiology), Panima Publishers, New Delhi, 2ndedition, 2003.
2. A.H.Patel., Industrial microbiology, Macmillan Publishers India, 2nd edition (2012).
REFERENCES
1. Prescott and Dunn, Industrial Microbiology, CBS Publishers, New Delhi, 4th
Edition, 1987
2. Young, M.Y., Comprehensive Biotechnology Vol. 1-4, Pergamon Press, Oxford, 1st
Edition, 1985
3. Stanbury, P.F., and Whitaker, A., Principles of Fermentation Technology, Pergamon
Press, Oxford, 2nd Edition, 2005.
L T P C
BIT215 BIOINFORMATICS AND COMPUTATIONAL BIOLOGY
3 1 0 4
Course Outcomes:
CO1: Describe the use of computers in storing, retrieving and annotating biological
information
CO2: Access, search and retrieve information from various biological databases
CO3: Comparatively analyze DNA and Protein sequences.
CO4: Perform phylogenetic analyses and determine the evolutionary relationship between
organisms
CO5: Explain the algorithms to predict primary, secondary and tertiary structure of proteins
from their sequences

CO1 H M
CO2 H M H
CO3 H M M
CO4 H M
CO5 H M
INTRODUCTION
Bioinformatics, Scope and applications of bioinformatics, Computer Hardware, Computer
networks, Network Topology, Internet, Internet Protocols, FTP, HTTP, Search engine,
Operating systems, Programming languages.
BIOLOGICAL DATABASES
Introduction to databases - Data life cycle, DBMS; Biological databases- Classification of
biological databases- Primary and Secondary databases, Sequence and structure databases,
Specialized databases- Data submission and Information Retrieval from biological databases.
PATTERN MATCHING
Sequence Homology versus Sequence Similarity, Sequence Similarity versus Sequence
Identity, Sequence alignment- Local and global alignment, Pair wise sequence alignment,
multiple sequence alignment, Dot Plots, Dynamic programming, Substitution matrices- PAM
and BLOSSUM, Database similarity searching -FASTA and BLAST, Protein signatures,
Identification of Motifs and Domains in Multiple Sequence Alignment.
PHYLOGENETIC ANALYSIS
Molecular Evolution and Molecular Phylogenetics, Phylogenetic tree, Forms of Tree
Representation, Rooted and un-rooted trees, Phylogenetic Tree Construction Methods:
Distance based methods- NJ, UPGMA, Character based methods ‚ Maximum Parsimony,
Phylogenetic programs
STRUCTURAL BIOINFORMATICS
Protein structure basics, Protein structural visualization and comparison, Secondary structure
prediction- Chau-Fasman, GOR, Neural networks, Protein tertiary structure prediction-
Homology modeling, Threading and Fold recognition.
TEXT BOOKS
1. Jin Xiong, Essential Bioinformatics, Cambridge University Press, New York, 1st
Edition, 2006.
2. Mount, D. W., Bioinformatics Sequence and Genome Analysis, Cold Spring Harbor
Laboratory Press, 2nd Edition, 2001.
3. Bergeron, B, (2003) Bioinformatics Computing, Eastern Economy Edition, 2nd
Edition, Prentice Hall, NewYork,
REFERENCES
1. Lesk, A. M., Introduction to Bioinformatics, Oxford University Press, New York,
1st Edition, 2003.
2. Baldi, P., Brunak, S., Bioinformatics, A Machine Learning Approach, MIT press,
USA, 2nd Edition, 1998.
3. Attwood, T.K., Perry smith, D.J., Introduction to Bioinformatics, Oxford University
Press, New York, 1st Edition, 2002.
L T P C
BIT216 PROTEIN SCIENCE AND ENGINEERING
3 0 0 3
Course Outcomes:
CO1: Explain and compare the different level of protein structure and their interdependence
and protein folding
CO2: Describe the regulation of gene expression control and function of proteins with an
examples of proton pump and photoreaction centre
CO3: Explain the theoretical knowledge of cloning of a gene on expression vector and
purification of proteins with various column
CO4: Describe various bioinformatics tools which are involved in phylogenetic analysis,
structure and functional prediction of proteins
CO5: Describe the protein engineering techniques how to utilize in industrial biotechnology

CO1 H M M M H M M M
CO2 H M M M M M M M
CO3 H M H H M M M
CO4 H M M M M
CO5 H M M M M H M M H
STRUCTURE OF PROTEINS
Primary structure - Peptide mapping, peptide sequencing, automated Edman method and
mass spectrophotometer - high throughput protein sequencing setup - Secondary structure -
alpha, beta and loop structures, alpha-turn-alpha, beta-turn-beta (hairpin), beta-sheets, alpha-
beta-alpha, topology diagrams, up and down and TIM barrel structures, nucleotide binding
folds, prediction of substrate binding sites - Tertiary structure - Domains, folding,
denaturation and renaturation - Overview of methods to determine 3D structures - Quaternary
structure - Modular nature, formation of complexes
STRUCTURE - FUNCTION RELATIONSHIP

DNA binding proteins - Prokaryotic transcription factors, helix-turn-helix motif in DNA
binding, Trp repressor, eukaryotic transcription factors, Zn fingers, helix-turn helix motifs in
homeodomain, leucine zippers - Membrane proteins - General characteristics, trans-
membrane segments, prediction - Bacteriorhodopsin and photosynthetic reaction center-
Immunoglobulin - IgG light chain and heavy chain architecture - Abzymes and enzymes-
serine proteases, understanding catalytic design by engineering trypsin, chymotrypsin and
elastase, substrate - Assisted catalysis other commercial applications
STRUCTURE PREDICTION AND MODELING

Structure prediction and modeling proteins - Molecular graphics in protein engineering -
Dynamics and mechanics - Drug-protein interactions and design
DATA ANALYSIS METHODS

Methods in protein data base analysis - theory, interactive graphics programme, perturbation
ENGINEERING OF PROTEIN
Protein engineering - Proteins design and engineering ‚ site-directed mutagenesis - strategies
to alter catalytic efficiency ‚ stabilization of industrial enzymes - Design of biotechnology
applicable yeast carboxypeptidase, ß-glycoside hydrolases - Specificity and stability in
glucoamylase - Engineering proteins for degradation of recalcitrant compounds and
biosensors
TEXT BOOKS
1. Branden, C. and Tooze, R., Introduction of Protein structure, Garland, 2nd Edition,
1999.
2. Lilia Alberghina., Protein Engineering in Industrial Biotechnology, Harwood
Academic publishers, Netherland, Reprint, 2003
REFERENCES
1. Creighton, T.E., Proteins, WH Freeman, New York, 2nd Edition, 1993
2. Donald Voet, Judith G. Voet, Charlotte W. Pratt, Fundamentals of Biochemistry
Life at molecular level, New York: John Wiley and sons, Inc, 4th edition, 2011.
L T P C
BIT288 COMPUTATIONAL BIOLOGY LABORATORY
0 0 3 2
Course Outcomes:
CO1: Access,search and retrieve information from various Biological databases
CO2: Perform database similarity search using online tools.
CO3: Use online tools for sequence analysis, alignment and comparison to find out sequence
similarity
CO4: Predict primary, secondary and tertiary structure of proteins using online proteomic
tools.
CO5: Construct phylogenetic trees form DNA / Protein sequences using specialized software

CO1 H M M H M
CO2 H M M
CO3 H M M
CO4 H M M
CO5 H M
1. Basic Unix/Linux commands
2. Exploring the integrated database system at NCBI server and querying the
PUBMED and GenBank databases using the ENTREZ search engine
3. Exploring & querying UniProt database.
4. Search & retrieval: PDB databases
5. Exploring the integrated database system at EBI server and searching the EMBL
Nucleotide database using the SRS search engine
6. Retrieve the database using Sequence Retrieval System (SRS and Expasy )
7. Pair-wise global alignments of protein and DNA sequences using Needleman-
Wunsch algorithm & interpretation of results to deduce homology between the
sequences, use of scoring matrices
8. Pair-wise local alignments of protein and DNA sequences using Smith-Waterman
algorithm and interpretation of results
9. Find the hydrogen bonding interaction using Chem2pac software.
10. Using Mega4 software draw Phylogenetic tree for the given sequences
(Protein/DNA).
11. Generate the secondary structural elements from amino acid sequence using various
methods.
12. Write any simple program to retrieve the sequence through internet.
L T P C
CHE291 CHEMICAL ENGINEERING LABORATORY
0 0 3 2
Course Outcomes:
CO1: Calibrate Flow meters
CO2: Calculate friction factor in pipes & packed beds
CO3: Demonstrate particle filtration and particle classification studies
CO4: Estimate overall heat transfer coefficient in heat exchangers
CO5: Employ mass transfer operations such as distillation and adsorption

CO1 H H M M
CO2 H H M M
CO3 H H M M
CO4 H H M M
CO5 H H M M
1. Flow measurement - Variable head and variable area meters (Venturi, orifice,
rotameter) - Calibration curve and determination of discharge coefficient
2. Pressure drop in straight pipes
3. Pressure drop in annular pipes
4. Pressure drop in packed columns - verification of Ergun equation
5. Fluidization - Calculation of minimum fluidization velocity
6. Filtration - plate and frame Filter, leaf filter and rotary vacuum filter
7. Heat exchanger - shell and tube, double pipe heat exchanger, calculation of
effectiveness and efficiency
8. Simple distillation
9. Steam distillation
10. Distillation in packed column
11. Liquid-liquid equilibria in extraction
12. Adsorption equilibrium
13. Transient heat conduction
14. Free and forced convection heat transfer
15. Batch sedimentation Test
SEMESTER V
L T P C
BIT303 BIOPROCESS PRINCIPLES
3 1 0 4
Course Outcomes:
CO1: Explain fermenter design
CO2: List the roles of a bioprocess engineer in the bioprocess industry
CO3: Summarize the role of medium formulation and optimization in fermentation processes
CO4: Describe sterilization kinetics and the various modes of sterilization
CO5: Express microbial growth kinetics in various modes of fermentation
CO6: Apply metabolic stoichiometry and energetics data in assessing and optimizing
fermentation process

CO1 H M
CO2 M H
CO3 M M H
CO4 M M
CO5 H M M
CO6 M M
OVERVIEW OF FERMENTATION PROCESS

Overview of fermentation industry - General requirements of fermentation processes, basic
configuration of fermenter and ancillaries, main parameters to be monitored and controlled in
fermentation processes - Role of bioprocess engineer in the biotechnology industry - Concept
of bioprocess outline of an integrated bioprocess and the various unit operations involved in
bioprocesses - Generalized process flow sheets - Modern applications of biotechnological
processes
MICROBIAL MEDIA AND MEDIUM OPTIMIZATION

Criteria for good medium - Medium requirements for fermentation processes, carbon,
nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements ‚ Medium
formulation of optimal growth and product formation, examples of simple and complex
media - Design of various commercial media for industrial fermentations - Medium
optimization methods, Plackett-Burman and Response Surface Methodology
STERILIZATION KINETICS
Thermal death kinetics of microorganisms ‚ Types of sterilization process, Batch and
continuous heat sterilization of liquid media - Filter sterilization of liquid media - Air
sterilization and design of sterilization equipment
METABOLIC STOICHIOMETRY AND ENERGETICS

Stoichiometry of cell growth and product formation - Elemental balances, degrees of
reduction of substrate and biomass, available electron balances - Yield coefficients of
biomass and product formation, maintenance coefficients, energetic analysis of microbial
growth and product formation - Oxygen consumption and heat evolution in aerobic cultures,
Thermodynamic efficiency of growth
KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION

Modes of operation - Batch, fed batch and continuous cultivation - Chemostat -Turbidostat -
Introduction to unstructured models for growth and product formation - Simple unstructured
kinetic models for microbial growth - Monod model - Growth of filamentous organisms -
Product formation kinetics - Substrate and product inhibition on cell growth and product
formation
TEXT BOOKS
1. Shuler, M.L. and Kargi, F., Bioprocess Engineering-Basic Concepts, Prentice Hall
Pvt. Ltd., New Delhi, 2nd Edition, 2004.
2. Peter, F., Stanbury ., Stephen, J., Hall and A. Whitaker., Principles of Fermentation
Technology, Elsevier, Science and Technology Books, New Delhi, 2nd Edition, 2005.
3. Doran, P.M., Bioprocess Engineering Principles, 2nd Edition, Academic Press (An
Imprint of Elsevier), New Delhi, 2nd Edition, 2013.
REFERENCES
1. Bailey, J.E. and Ollis, D.F., Biochemical Engineering Fundamentals, McGraw Hill
Publishers, New Delhi, 2nd Edition, 1986.
2. James Lee, M., Biochemical Engineering, Prentice-Hall Inc Publishers, Delhi. 1st
Edition, 1992.
L T P C
BIT304 GENETIC ENGINEERING
3 1 0 4
Course Outcomes:
CO1: Summarize the enzymes involved in cloning and restriction enzymes in recombinant
DNA technology
CO2: Describe different types of vectors such as plasmid, cosmid, phage and YAC.
CO3: Explain the cloning of a gene in different types of vectors and its applications
CO4: Illustrate construction and screening of cDNA and genomic libraries.
CO5: Describe cloning and transformation of Ti vectors in plants

CO1 H M
CO2 H M M
CO3 H M M M H
CO4 H M M M
CO5 H H M H M H M
MOLECULAR TOOLS FOR GENE CLONING

Restriction enzymes- Modifying enzymes - DNA and RNA polymerases - Safety guidelines
of recombinant DNA research
VECTORS FOR GENE CLONING

Introduction to cloning vectors- plasmid vectors (high copy and low copy)-Phage vectors,
cosmid vectors - phasmid vectors - BAC vectors and YAC vectors - Insect, yeast and
mammalian vectors
CLONING AND EXPRESSION OF RECOMBINANT PROTEIN

Cloning strategies- restriction digestion - blunt and cohesive end ligation ‚ design of linkers
and adaptors - cloning after homopolymer tailing; Strategies for cloning PCR products -
creation of restriction sites, Plasmid expression vectors-General features - promoters used in
expression vectors -cloning of genes in correct reading frame in expression vector- Strategies
for purification of recombinant proteins
CONSTRUCTION & SCREENING OF LIBRARIES

Construction of cDNA library- construction subtractive cDNA library ‚ construction of
genomic DNA library ‚ BAC library ‚ YAC library - Screening of libraries with DNA
probes and anti-sera - methods of nucleic acid sequencing - Sangers method
APLLICATION OF RECOMBINANT DNA TECHNOLOGY

Cloning in plants - Ti plasmid - Transgenic and knockout animals
TEXT BOOKS
1. Primrose, S., B. and Twyman, R., M., Principles of Gene Manipulation and
Genomics, Blackwell Publishing Co., 7th Edition, 2006.
2. Brown, T.A.,Gene Cloning and DNA analysis-An Introduction, BlackwellScience,
2nd Edition, 2001.
REFERENCES
1. Lodge, J., Lund, P., and Minchin, S., Gene Cloning, Taylor & Francis Group
ISBN: 0-7487-6534-4, 2007
L T P C
BIT322 ENZYME TECHNOLOGY
3 1 0 4
Course Outcomes:
CO1: Explain the characteristics and catalytic mechanisms of enzymes
CO2: Identify enzyme inhibition patterns and determine kinetics of single substrate enzyme
catalyzed reactions
CO3: Characterize enzymes and design enzyme assays
CO4: Describe immobilization techniques,and their principles, advantages and disadvantages
CO5: Suggest a preliminary design for biosensors

CO1 H M M
CO2 H M M
CO3 H M H M M
CO4 H M
CO5 H H H M
INTRODUCTION TO ENZYMES
Classification of enzymes - Mechanisms of enzyme action, concept of active site and
energetic of enzyme substrate complex formation - Specificity of enzyme action - Principles
of catalysis - Collision theory, transition state theory - Role of entropy in catalysis - Types of
enzymes - constitutive enzyme, induced enzymes, intracellular and extracellular enzymes -
Application of enzymes in food, pharmaceutical and other industries - Enzymes for analytical
and diagnostic applications
KINETICS OF ENZYME ACTION

Kinetics of single substrate reactions - Estimation of Michaelis -Menten parameters,
Turnover number ‚ Multi-substrate reactions, Mechanisms and kinetics - Types of inhibition,
Kinetic models, Substrate and product inhibition - Allosteric regulation of enzymes, The
Monod-Changeux-Wyman model and the Koshland-Nemethy-Filmer model - pH and
temperature effect on enzyme activity
PURIFICATION AND CHARACTERIZATION OF ENZYMES FROM NATURAL

SOURCES
Methods of production of enzymes, Extraction of enzymes from various sources like plant,
animal and microbial sources, soluble enzymes, and membrane bound enzymes - Nature of
extraction medium - Purification of enzyme - Criteria of purity - Determination of molecular
weight of enzymes.
ENZYME IMMOBILIZATION
Physical and chemical techniques for enzyme immobilization - adsorption, matrix
entrapment, encapsulation, cross-linking, covalent binding with example - Advantages and
disadvantages of different immobilization techniques - Overview of applications of
immobilized enzyme systems
BIOSENSORS
Introduction to biosensors ‚ History - Types and design of enzyme electrodes ‚ Biosensors
applications in industry, healthcare and environment
TEXT BOOKS
1. Chaplin, M. and Bucke, C. (1990). Enzyme Technology, 1st Edition, Cambridge
University Press, London, 1st Edition, 1990.
2. Palmer, T., Enzymes: Biochemistry Biotechnology and Clinical Chemistry, East West
Press Pvt Ltd, New Delhi, 5th Edition, 2001.
REFERENCES
1. James Lee, M. (1992). Biochemical Engineering, 1st Edition, Prentice-Hall Inc
Publishers, Delhi, 1st Edition, 1992.
2. Blanch, H. W. and Clark, D.S., Biochemical Engineering, CRC Press, USA, 2nd
Edition, 1997.
3. Zubay, G., Biochemistry, 4th Edition, McGraw Hill Publishers, New Delhi, 4th
Edition, 1999.
L T P C
CHE357 REACTION ENGINEERING FOR BIOTECHNOLOGISTS
3 0 0 3
Course Outcomes:
CO1: Describe the kinetics of reactions
CO2: Design equations to determine the performance of ideal reactors
CO3: Create various models for describing non- ideal behavior of reactors
CO4: Analyze performance of combined reactors
CO5: Explain adsorption and desorption phenomena in heterogeneous systems.

CO1 H M
CO2 H M
CO3 H M
CO4 H M
CO5 H M
REACTION KINETICS
Law of mass action, rate equation, elementary, non-elementary reactions and their
mechanisms, theories of reaction rate and temperature dependency, analysis of experimental
reactor data, evaluation of rate equation, integral and differential analysis for constant and
variable volume system, fitting of data complex reaction mechanism, searching of reaction
mechanism
IDEAL REACTORS
Isothermal and non isothermal homogeneous reactor systems, adiabatic reactors, Design of
batch and continuous reactors, optimum temperature progression.
NON IDEALITY IN REACTORS

RTD in non-ideal flow; non-ideal flow models, conversion in non-ideal reactors.
HOMOGENEOUS REACTION SYSTEM

General mole balance equation: batch, plug flow, mixed flow reactor (steady & unsteady
state condition).Design for homogeneous systems, batch, stirred tank and tubular flow
reactor, fluidized bed, fixed bed. Design of reactors for multiple reactions, combination
reactor system, size comparison of reactors.
HETEROGENEOUS REACTION SYSTEM

Rate equations for heterogeneous reactions, nature of catalysis, adsorption isotherms and
rates of adsorption, desorption and surface reaction analysis of rate equation and rate
controlling steps, surface area and pore-volume distribution, catalyst preparation, Diffusion
within catalyst particle, effective thermal conductivity mass and heat transfer within catalyst
pellets.
TEXT BOOKS
1. Levenspiel, O. (2009) Chemical Reaction Engineering, 3rd Edition, John Wiley.
2. Smith, J.M. (1981) Chemical Engineering Kinetics, 3rd Edition, McGraw-Hill.
REFERENCES
1. Missen, R.W., Mims, C.A. and Saville, B.A. (1998) Introduction to chemical reaction
engineering and kinetics, John Wiley & Sons.
2. Fogler. H.S. (2005) Elements of Chemical reaction engineering, 4th edition, Prentice
Hall
L T P C
BIT387 BIOPROCESS LABORATORY
0 0 4 2
Course Outcomes:
CO1:Plan an experiment to produce an enzyme using an appropriate microbial strain
CO2:Screen amylase producing bacteria from soil samples
CO3:Optimize the effect of pH, temperature, substrate concentration and reaction time on
amylase activity
CO4:Use immobilization techniques
CO5:Study the activity of enzymes and the kinetics of different enzymatic reactions

CO1 H M H H H
CO2 H H M H H M
CO3 H H H H H M H H
CO4 H H H M H H M H
CO5 H H H M H M H
1. Determination of bacterial growth by turbidity measurement; measurement of
biomass by dry weight and wet weight
2. Growth kinetics of bacteria - calculation of specific growth rate, yield coefficient in
shake flask culture
3. Growth of yeast - estimation of biomass, calculation of specific growth rate, yield
coefficient in shake flask culture
4. Estimation of enzyme activity (amylase, protease) calculation of specific activity
5. Enzyme kinetics ‚ Michaelis-Menton parameters
6. Effect of pH and temperature on enzyme activity
7. Enzyme inhibition kinetics
8. Immobilization of yeast cells
9. Enzyme immobilization - gel entrapment; kinetics of immobilized enzyme reactions
10. Bioconversion studies with immobilized enzyme using packed - bed reactors.
11. Extraction of proteolytic enzyme from papaya latex
12. Extraction of invertase enzyme from yeast cells
13. Estimation of invertase activity
L T P C
BIT388 GENETIC ENGINEERING LABORATORY
0 0 4 2
Course Outcomes:
CO 1: Isolate the plasmid DNA from bacterial cells
CO 2: Design the setting up of restriction digestion of DNA
CO 3: Isolate genomic DNA from prokaryotic and eukaryotic cells
CO 4: Demonstrate the Southern blotting technique
CO 5: Formulate PCR reaction conditions

CO 1 H M M H M M H M
CO 2 H M H M M H
CO 3 H M M H M M H M M
CO 4 H H M M H
CO 5 H M M H M M H M M
1. Isolation of chromosomal DNA from bacteria
2. Sub-cloning of a sucrase gene in E. coli ‚ (restriction digestion, gel isolation and
ligation, transformation and screening of recombinants)
3. Polymerase Chain Reaction ‚ (identification of microorganisms using 16srRNA
sequence)
4. Isolation of RNA
5. Southern blotting
6. Northern blotting
7. Western blotting
8. Colony hybridization
9. Site-directed mutagenesis using sequence specific primers
SEMESTER VI
L T P C
BIT305 BIOCHEMICAL ENGINEERING
3 1 0 4
Course Outcomes:
CO:1 Explain ideal and non-ideal behaviour of reactors.
CO:2 Describe the configurations and applications of various bioreactors.
CO:3 Suggest scale up of design parameters for bioreactors.
CO:4 Illustrate immobilization techniques and their principles advantages and disadvantages.
CO:5 Explain the models of cell growth

CO1 H M M
CO2 H
CO3 H M M
CO4 H M
CO5 H M M
ANALYSIS OF BIOREACTORS
Stirred Tank Reactor- non-ideal behavior, RTD and stability analysis - Tanks-in-series and
dispersion models - Application to design of continuous sterilizers - Design and operation of
novel bioreactors -airlift reactor, bubble column reactors ‚ Stability analysis of bioreactors
SCALE UP OF BIOREACTORS
Regime analysis of bioreactor processes - Oxygen mass transfer in bioreactors - Microbial
oxygen demands - Methods for the determination of mass transfer coefficients- mass transfer
correlations - Scale up criteria for bioreactors based on oxygen transfer - Power consumption
and impeller tip speed
MODELLING AND SIMULATION OF BIOPROCESSES

Study of structured models for analysis of various bioprocesses - Compartmental models,
cybernetic models - Models of cellular energetic and metabolism - Single cell models,
plasmid replication and plasmid stability model - Dynamic simulation of batch, fed batch,
steady and transient culture metabolism - Model simulation using MATLAB-SIMULINK
and ISIM software packages
MODERN BIOTECHNOLOGICAL PROCESSES

Recombinant cell culture processes - Guidelines for choosing host-vector systems, plasmid
stability in recombinant cell culture - Limits to over expression - Bioreactor strategies for
maximizing product formation - Bioprocess design considerations for plant and animal cell
cultures
BIOREACTOR CONSIDERATIONS IN ENZYME SYSTEMS

Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions -
Formulation of dimensionless groups and calculation of effectiveness factors - Design of
immobilized enzyme reactors ‚ Packed bed, fluidized bed and membrane reactors
TEXT BOOKS
1. Harvey W. Blanch., Douglas S. Clark, ƒBiochemical Engineering„, Marcel Decker
Inc. 2007.
2. Bailey, J.E., Ollis, D.F., Biochemical Engineering Fundamentals, McGraw Hill
Publishers, New Delhi, 2nd Edition, 1986.
REFERENCES
1. Shuler, M.L. and Kargi, F., Bioprocess Engineering-Basic Concepts, Prentice Hall
Pvt. Ltd., New Delhi, 2nd Edition, 2004.
2. Doran, P.M., Bioprocess Engineering Principles, Academic Press (An Imprint of
Elsevier), New Delhi, 2nd Edition, 2005.
3. James Lee, M., Biochemical Engineering, Version 2.1, PHI, USA, 2002.
L T P C
BIT306 IMMUNOLOGY
3 1 0 4
Course Outcomes:
CO1: Understand the differentiation of hematopoietic stem cell, complementary cascade and
anatomy of lymphoid organ
CO2: Explain the structure, function, and genetic regulation of antibody and their
development, and activations
CO3: Describe various mechanisms of antigen presenting cells and how to regulate
phagocytosis and macrophage
CO4: Explain the different types hypersensitive reactions and cytokine molecules
CO5: Describe molecular mechanism of Graft rejection, immunosuppressive drugs, and
autoimmune diseases

CO1 M H M M M H M M
CO2 H M M M M M M M
CO3 M M H M M
CO4 M M M H M M M M
CO5 H M M H M H M
THE IMMUNE SYSTEM

Introduction - Lymphocytes, their origin and differentiation - Antigens, their structure and
classification - Complement and their biological functions -Types and regulation of immune
responses, anatomy of immune response
HUMORAL IMMUNITY
B-Lymphocytes and their activation - structure and function of immunoglobulin,
immunoglobulin classes and subclasses, genetic control of antibody production - Hybridoma
technology and diagnosis - Idiotope and antibodies - Major histocompatibility Complex,
Production of polyclonal and monoclonal antibody; antigen ‚ antibody interactions-
immunostaining ‚ immunocytochemistry ‚ in situ hybridization
CELLULLAR IMMUNOLOGY
Thymus derived lymphocytes (T Cells), their classification - Antigen presenting cells (APC),
macrophages, langerhans cells, their origin and function - Mechanisms of phagocytosis,
identification ‚ antigen processing and presentation
IMMUNITY TO INFECTION
Hypersensitivity reactions - Mechanisms of T cell activation - Cytokines and their role in
immune response ‚ Macrophage activation and granuloma formation
TRANSPLANTATION AND AUTOIMMUNITY

Graft versus host reaction, Graft rejection - evidence and mechanisms of graft rejection -
Prevention of graft rejection - Immunosuppressive drugs - HLA and disease - Mechanisms of
immunity to tumour antigens-Autoantibody in humans - Pathogenic mechanisms -
Experimental models of autoimmune disease - Treatment of auto immune disorders
TEXT BOOKS
1. Janes Kuby., Immunology, WH Freeman and Company, Newyork.,6th Edition, 2006.
2. Roitt, I., Essential Immunology, Blackwell Scientific Publications, Oxford, 11th
Edition, 2006.
REFERENCES
1. Benjamin, E., Leskowitz, S., Immunology ‚ A Short Course, Wiley Liss, New York,
3rd revised Edition, 1996.
2. Jeneway, C. A Jr. and Travers, P.T., Immunobiology, Blackwell Scientific
Publishers, 6th Edition, 2004.
L T P C
BIT389 IMMUNOLOGY LABORATORY
0 0 3 2
Course Outcomes:
CO 1: To identify the blood group of unknown sample by agglutination test
CO 2: To perform radial and double immuno-diffusion
CO 3: To handle animals for bleeding techniques
CO4: To demonstrate rocket immuno-electrophoresis
CO5: Execute clinical tests such as ELISA and Widal test

CO 1 H H H M M M H
CO 2 H M M H H M
CO 3 H M H M M M M H
CO4 H M M H H M M
CO5 H H H H H M M M H M
1. Identification of blood group
2. Handling of animals and bleeding techniques
3. Testing for typhoid antigens by Widal test
4. Immunoelectrophoresis
5. Rocket Immunoelectrophoresis
6. Outcherlony double diffusion
7. Radial Immunodiffusion
8. Affinity chromatography for antibody purification
9. ELISA-DOT and plate ELISA
10. Sandwich ELISA
11. Quantitative precipitation Assay
12. Latex agglutination Test
13. Western blot
L T P C
BIT390 BIOCHEMICAL ENGINEERING LABORATORY
0 0 6 2
Course Outcomes:
CO:1Compute residence time distribution for PFR and MFR.
CO:2 Determine mixing time in a reactor.
CO:3 Optimize media by Plackett Burman method
CO:4 Simulate batch and fed batch fermentation
CO:5 Study growth and product formation kinetics

CO:1 M M M H
CO:2 M H
CO:3 H H M
CO:4 H M M
CO:5 H H H M M
1. Demonstration of various bioreactor configurations, parts and integrated process
control systems.
2. Batch sterilization design and Thermal death kinetics
3. Medium optimization - Plackett Burman design
4. Medium optimization - Response surface methodology
5. Estimation of KLa by sulphite oxidation method.
6. Estimation of mass transfer coefficient and power number correlation
7. Estimation of mixing time in batch reactor.
8. Residence time distribution analysis.
9. Estimation of overall heat transfer coefficient.
10. Batch cultivation, estimation of KLa ‚ Dynamic gassing method, exhaust gas analysis
‚ carbon balancing, gas balancing.
11. Fed batch cultivation, exhaust gas analysis ‚ carbon balancing, gas balancing
12. Continuous cultivation ‚ x-d diagram, pulse and shift method, exhaust gas analysis ‚
carbon balancing, gas balancing
13. Model simulation using MATLAB-SIMULINK, BMS and ISIM software packages
SEMESTER VII
L T P C
BIT 401 ANIMAL BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Explain animal cell culture media and animal cell culture techniques
CO2: Describe expression vectors and production of recombinant products using animal
cells
CO3: Apply biotechnological methods for basic research
CO4: Apply reproduction methods with particular reference to gamete and embryo
manipulation techniques, production of transgenic animals and cloning
CO5: Discuss manipulation strategies to improve livestock production including meat and
milk production

CO1 H H
CO2 H H M
CO3 M H
CO4 H M M
CO5 H H M
ANIMAL CELL CULTURE

Media for culturing cells and tissues - Chemically defined and serum free media for cell
culture; Sterilization of various equipments and apparatus - Cell culture substrates - Animal
cell culture; types and methods - Development of cell lines; Development, Maintenance,
Preservation and Characterization of animal cells ‚ Scaling up of animal cell cultures - Cell
culture as source of valuable products-Protein production by genetically engineered
mammalian cell lines, Stem cells and their applications.
GENE TRANSFER METHODS

Gene transfer methods - Virus mediated methods; Biology and Construction of viral vectors
like SV40, adenovirus, lentivirus, vaccinia virus, herpes virus, and adeno associated virus,
baculovirus ‚ Transfection methods; stable and transient methods
MICROMANIPULATION OF EMBRYO•S AND EMBRYO TRANSFER

Micromanipulation technology; Artificial insemination, Superovulation, Embryo transfer,
Invitro fertilization - Pregnancy diagnosis - Sexing of embryos, Embryo splitting;
Cryopreservation of embryo - Cloning and SCNT - Breeding of farm animals
TRANSGENIC ANIMALS
Concepts of transgenic animal technology - Various strategies for the production of
transgenic animals and their importance in biotechnology; pronuclear microinjection,
embryonic stem cells and somatic cell nuclear transfer in the production of transgenic
animals. - Transgenic animals as bioreactors for producing pharmaceutically important

compounds and therapeutics etc. Role of gene knock out and gene knock in mice model for
studying human genetic disorder
BIOTECHNOLOGY IN ANIMAL PRODUCTION

Manipulation of Growth hormone; Somatotropic hormone and Thyroid hormone - Probiotics
as growth promoters; Ideal characteristics of probiotics; Mode of action and uses of
probiotics-Manipulation of lactation -Lactogenesis-galactopoiesis, wool growth and rumen
microbial digestive system.
TEXT BOOKS
1. Davis, D., Animal Biotechnology, National Academic Press, Washington, 1st Edition,
2002.
2. Ramadoss, P., Animal Biotechnology: Recent Concepts and Developments, MJb
Publishers, Chennai, 1st Edition, 2008.
REFERENCES
1. Freshney, R. I., Culture of Animal Cells: A manual of Basic technique, John ‚ Wiley
and sons, 6th Edition, 2010.
2. Masters, J.R.W., Animal Cell Culture: Practical Approach, Oxford University Press,
New York, 3rd Edition, 2000.
3. Holland, A. and Johnson, A., Animal Biotechnology and Ethics, Springer Verlag,
New York, 1st Edition, 1998.
L T P C
BIT402 PLANT BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO 1: Outline the method of creating transgenic plants in general
CO 2: Explain how to make commercially important compounds using plant tissue culture
CO 3: Describe how micropropagation is carried out and its advantages
CO4: Differentiate plant breeding and genetic engineering approaches
CO5: Report the strategy and advantages of creation of BT cotton

CO 1 M H M H M M M M M
CO 2 M H M M M M M H
CO 3 M H M M M M M H
CO4 H H H H M M M H M
CO5 H H H M M M M H M
INTRODUCTION TO PLANT BIOLOGY

Photosynthetic bacteria and Blue-green Algae. Autotrophs, heterotrophs and evolution of
plants from algae. Special features and organization of plant cells, Chloroplast DNA,
photosynthesis, Mitochondrial DNA, and chromatin and chromosome structure.
PLANT TISSUE CULTURE

Plasticity and totipotency, Plant cell culture media, Plant growth regulators, Organogenesis
Culture types: Callus, cell-suspension culture, protoplasts, root culture, hairy root culture and
production of secondary metabolites, Plant regeneration by somatic embryogenesis: Direct
and Indirect. Case study: Cereal regeneration via somatic embryogenesis from immature
embryos and Micropropagation
PLANT BREEDING BASICS AND PLANT TRANSFORMATION

Simple and complex inheritance, Value of plant variety depends on many traits, back cross,
Molecular Markers: RFLP and PCR based SSR markers, Marker-Assisted selection, Hybrid
seeds production. Agrobacterium biology and T-DNA transfer, Binary vector system,
Agrobacterium-mediated plant transformation, Direct DNA transfer methods in plants -
particle bombardment method.
APPLICATIONS OF PLANT GENETIC ENGINEERING

Herbicide tolerant plants: Different strategies to achieve, strategy to generate glyphosate
tolerant plants and their related problems. Mechanism of insecticidal crystal protein of
Bacillus thuringiensis, strategy to generate BT cotton transgenic plants; their problems and
solutions. Disease resistance: against bacterial, fungal and viral pathogens, gene silencing,
applications in virus resistance and golden rice.
MOLECULAR FARMING AND PLANT GENOMICS

Molecular farming of proteins, Plant vaccines, custom-made antibodies, case study:
Bioplastics. Genome size and organization, Arabidpsis genome sequencing project
technology and applications; Biotechnological implications of Arabidopsis genome initiative,
crop plant genome sequencing.
TEXT BOOKS
1. Slater A., Nigel W., Scott, and Fowler MR., Plant biotechnology: The Genetic
Manipulation of Plants, Oxford University Press, London, 2nd Edition, 2008.
2. Neal Stewart, Jr., Plant Biotechnology and Genetics: Principles, Techniques, and
Applications. John Wiley & Sons Inc. USA, 2008.
3. Chawla HS ., Introduction to Plant Biotechnology, Oxford & IBH Publishing Co. Pvt.
Ltd. New Delhi, 2nd Edition, 2003.
L T P C
BIT403 DOWNSTREAM PROCESSING
3 1 0 4
Course Outcomes:
CO1: Describe the advantages of bioprocesses as well as explain the principles of various
separation processes
CO2: Explain the various concepts of centrifugal separation and diffusion-based processes,
such as dialysis
CO3: Demonstrate knowledge of the principles of pressure-driven processes, such as reverse
osmosis, and adsorption-desorption phenomena
CO4: Explain the principles of aqueous two-phase extraction and the various techniques of
protein precipitation.
CO5: Explain the principles of planar chromatography and those of the various finishing
operations used for bioproducts

CO1 H H M H M M
CO2 H M H
CO3 H H H M
CO4 H M H M
CO5 H M M M M
INTRODUCTION TO DOWNSTREAM PROCESSING

Advantages of bioprocesses over chemical processes - Range and characteristics of
bioproducts ‚ Importance of downstream processing in biotechnology ‚ Characteristics of
fermentation broths ‚ Overview of bioseparations ‚ Case studies.
PHYSICAL METHODS OF SEPARATION

Batch Filtration: Theory, Filter Media and Equipment ‚ Continuous Filtration ‚
Centrifugation: Settling of solids, Centrifugal Sedimentation, Centrifugal Decantation and
Centrifugal Filtration ‚ Scale-up of centrifugation ‚ Membrane separation processes:
Classification and theoretical considerations, Equipment and types of membrane processes
ISOLATION OF PRODUCTS
Cell disruption: Chemical, Mechanical and Enzymatic methods ‚ Adsorption: Process and
Isotherms ‚ Adsorption techniques: Batch, in CSTR and Fixed bed ‚ Analysis and scale-up
‚ Extraction: Solvent extraction principles, Equipment for extraction, Modes of extraction ‚
Aqueous two-phase extraction ‚ Supercritical fluid extraction ‚ Precipitation of proteins by:
Isoelectric precipitation, addition of salts, addition of organic solvents, addition of non-ionic
polymers and ionic polyelectrolytes - Scale-up of precipitation.
CHROMATOGRAPHIC METHODS
Basic principles and classification ‚ Reversed-phase chromatography ‚ Hydrophobic
interaction chromatography - Ion-exchange chromatography ‚ Size-exclusion
chromatography ‚ Bioaffinity chromatography.
FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS
Crystallization: Theory, Practice and Equipment for crystallization ‚ Drying: Theoretical
considerations and drying equipment ‚ Freeze Drying (Sublimation Drying or
Lyophilization) - Formulation
TEXT BOOK
1. B. Sivasankar., Bioseparations, Eastern Economy Edition, Prentice-Hall of India Pvt.
Ltd., New Delhi, 2006.
REFERENCES
1. Belter, P.A., Cussler, E.L. and Wei-Houhu., Bioseparations: Downstream Processing
for Biotechnology, Wiley Interscience Publications, Singapore, 1st Edition, 1988.
2. P.F. Stanbury, A. Whittaker and S.J. Hall, Principles of Fermentation Technology,
Butterworth Heinemann, USA, 2nd Edition, 2003.
L T P C
BIT491 DOWNSTREAM PROCESSING LABORATORY
0 0 6 2
Course Outcomes:
CO1: Carry out isoelectric precipitation of proteins from a protein mixture; perform
ultrasonication of cells and monitor kinetics of protein release
CO2: Explain the principles of microfiltration and homogenization and perform these
processes
CO3: Understand the principles of centrifugation and adsorption and carry out these
processes
CO4: Understand and explain reaction equilibria; be familiar with the physical properties of
filtration cakes
CO5: Explain the principles of the various types of planar and columnar chromatography

CO1 H H M H M
CO2 H M M H H
CO3 H H H
CO4 H H M
CO5 H H M
1. Solid liquid separation ‚ centrifugation, microfiltration
2. Cell disruptions techniques ‚ ultrasonication, French pressure cell
3. Cell disruption techniques ‚ dyno mill ‚ batch and continuous
4. Precipitation ‚ Ammonium sulphate Method

5. Ultra filtration separation
6. Aqueous two phase extraction of fermentation Broth
7. High resolution purification - Gel filtration chromatography
8. High resolution purification - Affinity chromatography
9. High resolution purification - Ion exchange chromatography
10. High resolution purification - Hydrophobic interaction chromatography.
11. Product Polishing - Spray drying, freeze drying
MAJOR ELECTIVES FOR III YEAR
L T P C
BIT 307 ENVIRONMENTAL BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Describe ecosystem and soil microbiology
CO2: Describe xenobiotics and the strategies to eliminate them from the environment
CO3: Explain the microbial techniques employed for wastewater management and treatment
CO4: Describe the methods of effluent treatment in various industries
CO5:. Explain the role of microorganisms in the extraction of metals, coal and petroleum.

CO1 M H M
CO2 H H M H M
CO3 H H M H M
CO4 H H H M
CO5 H H H H
INTRODUCTION
Introduction to ecosystem - Microbial flora of soil - Interaction among Soil microorganisms
‚ Bio geochemical cycle - Role of soil microorganisms
BIODEGRADATION AND BIOCONVERSION OF XENOBIOTIC COMPOUNDS

Xenobiotics - Persistence and biomagnification of xenobiotic molecules-Simple aromatics,
chlorinated polyaromatic compounds, petroleum products, pesticides and surfactants -
Mechanism of detoxification - oxidation, dehalogenation - Biotransformation of metals ‚
Bioremediation concepts, strategies and applications.
MICROBIAL TECHNOLOGY FOR WASTE WATER TREATMENT

Waste water characteristics - Sewage and waste water treatments systems; Primary,
secondary and tertiary treatments- Biological waste water treatment - Unit operations and
unit processes - Design and modeling of activated sludge process - Anaerobic digestion- ;
Bioreactors for waste water treatments
TREATMENT OF INDUSTRIAL WASTES

Physicochemical characteristics and treatment strategies for effluent generated by food and
dairy industries; Paper and pulp industries; Dyes and dye intermediate producing industries
and textile industries; Tanneries; pharmaceutical industries - Phytoremediation: Waste water
treatment using aquatic plants; Solid waste management.
BIOTECHNOLOGY FOR MANAGEMENT OF RESOURCES

Role of environmental biotechnology in management of resources- Reclamation of
wasteland- Biogas and biofuel production ‚ Biofertilizers. Biological control of insect pests;
Role of biopesticides/insecticides- Biotechnology and oil spills. Genetic manipulation for
biodegradation
TEXT BOOKS
1. Rittman, B and McCarty, P.L, Environmental Biotechnology: Principles and
Applications, McGraw- Hill, 2nd Edition, 2000.
2. Karnely, Chakrabarty, D, Omen, G.S., Biotechnology and Biodegradation,
Advances in Applied Biotechnology Series, Volume1, Gulf Publications Company,
London, 1st Edition, 1989.
3. Foster, C.F., John Ware, D.A., Environmental Biotechnology, Ellis Harwood Ltd., 1st
Edition, 1987.
REFERENCES
1. Young, M.Y., Comprehensive Biotechnology, Vol 1-4, Pergamon Press, Oxford, 1st
Edition, 1985.
2. Wanwright, M., An Introduction to Environmental Biotechnology, Springer Verlag,
London, 1st Edition, 1999.
BIT 308 SPECTROSCOPIC METHODS FOR STRUCTURE L T P C

DETERMINATION 3 0 0 3
ELECTROMAGNETIC AND QUANTUM THEORY OF RADIATION

Wave - Particle Duality ‚ Photons - Yukov•s Theory for Nature of Forces - Interaction of
Light with Matter - Transition Dipole Moment - Group Theory - Jablonsky Diagram
SPECTROSCOPY OF BIOMOLECULES
UV-Visible Absorption Spectroscopy - Beer-Lambert's law - Applications of UV-Visible
Difference Spectroscopy - Circular Dichroism in Protein Analysis - Fluorescence
Spectroscopy - Quantum Yield - Static and Dynamic Quenching of Fluorescence - Energy
Transfer ‚ Polarization Anisotropy - Time-resolved Fluorescence
VIBRATIONAL SPECTROSCOPY
IR, FTIR and Raman Spectroscopy of Biomolecules - Nuclear Magnetic Resonance
Spectroscopy - Chemical Shifts - Coupling Constants - Ring Currents - Paramagnetic Shifts,
Spin-Spin and Spin-lattice Relaxation Times ‚ NOE Chemical Exchange - Applications to
Biomolecular Structure and Dynamics
ELECTRON SPIN RESONANCE SPECTROSCOPY

Hyperfine splitting ‚ Zero-field splitting - Spin labels - Mass Spectrometry of Biomolecules
X-RAY DIFFRACTION
Crystal Systems and Space Groups - Miller Indices and Space Lattices - Diffraction of X-
rays and Bragg•s Law - Structure Determination of Biomolecules - Refinement and Accuracy
of X-ray Crystallographic Structures - Scanning Tunneling Microscopy - Atomic Force

Microscopy - Electron Microscopy of Biomolecules
TEXT BOOK
1. Cantor, C.R. and Schimmel, P.R., Biophysical Chemistry, Part-2, W.H.Freeman
Company, New York, 1st Edition, 1980.
REFERENCES
1. Lakowicz, J.R., Principles of Fluorescence Spectroscopy, Plenum Press,
Philadelphia, 3rd Edition, 2006.
2. Atkins, P.W. and Paula, J.De., Physical Chemistry, Oxford University Press, New
York, 9th Edition, 2010.
3. Willard, H., Merritt, L., Dean J.A., and Settle, F.A., Instrumental methods of analysis,
CBS publishers, New Delhi, 7th Edition, 1988.
4. Skoog, D.A., West, D.M., Holler, J.F and Crouch, S.R., Fundamentals of Analytical
Chemistry, Cengage India, 9th Edition, 2013.
L T P C
BIT 309 FOOD PROCESSING AND TECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Outline the main ways in which primary production of food is of importance to food
quality.
CO2: Describe the general features and importance of proteins, lipids and carbohydrates in
foods
CO3 Describe the major chemical reactions that occur during food processing and storage
CO4: Apply the basic knowledge of food ingredients and processing operations to describe
the production of cheese emulsions and milk powder
CO5: Identify the beneficial and detrimental roles played by microorganisms in the food
industry.

CO1 H H M H
CO2 H H M
CO3 H M H H
CO4 H H H M
CO5 M H H H
INTRODUCTION TO FOOD PROCESSING

Biotechnology in relation to the food industry - Nutritive value of food - Recent trends in
food biotechnology. Application of biotechnology in food
FOOD PRESERVATION
Principles involved in the use of sterilization, pasteurization and blanching - Thermal death
curves of microorganisms ‚ Canning - Frozen storage - Freezing characteristics of foods -
Microbial activity at low temperatures - Factors affecting quality of foods in frozen storage -
Irradiation preservation of foods
FOOD PROCESSING
Bioprocess of meat, fisheries, vegetables, Beverages, baking, diary product - Enzymes and
chemicals used in food processing , Baking (fungal -amylase for bread making; maltogenic -
amylases for anti-staling; xylanses and pentosanases as dough conditioners; lipases or dough
conditioning; oxidases as replacers of chemical oxidants; synergistic effect of enzymes);
HFCS (High Fructose Corn Syrup) - Biochemical engineering for flavor and food
production,
FOOD MICROBIOLOGY
Importance and significance of microorganisms in food science.Types of microorganisms
associated with food, its sources, Utilization of microorganisms in food industries - Genetic
manipulations, Fermented Food Products, mycoproteins
FOOD SPOILAGE
Characteristic features, dynamics and significance of spoilage of different groups of foods.
Food borne illness - Quality control - Case studies on biotechnology in the evolution of food
quality.
TEXT BOOKS
1. Lopez, G.F.G. and Canovas, G.V.B., Food Science and Food Biotechnology, CRC
Press, Florida, USA, 2003.
2. Roger, A., Gordan, B., and John, T., Food Biotechnology, Cambridge University
Press, USA, 1st Edition, 1989
3. Pelezar, M.I and Reid, R.D., Microbiology, McGraw Hill Book Company, New York,
5th Edition, 1993.
4. James, M.J. Modern Food Microbiology, CBS Publisher, 2nd Edition, 2000.
REFERENCES
1. George, J. B., Basic Food Microbiology, Springer Verlag, London, 2nd Edition, 1995.
2. James, M. J., Modern Food Microbiology, Springer Verlag, London, 7th Edition,
2006.
3. Frazier, W.C., Westhoff, D.C., Food Microbiology, McGraw-Hill Book Co, New
York, 4th Edition, 1988.
L T P C
BIT 310 PHARMACEUTICAL BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Describe the concept of drug discovery and drug development
CO2: Differentiate the mechanisms of Synthesis and secretion of recombinant proteins
CO3: Categorize various blood clotting disorders and blood-clotting factors
CO4: Explain the cell and tissue based therapies
CO5: Perform assays involving in protein based contaminants detection

CO1 M H M M
CO2 H H M M M H M
CO3 M M M H M H M
CO4 H M M H M M M
CO5 H H M M M M M
INTRODUCTION
History of the Pharmaceutical Industry ‚ Current Status and Future Prospects of
biopharmaceuticals ‚ Drug Discovery ‚ Drug Development ‚ Pharmaceuticals of Animal,
Plant and Microbial Origin ‚ Sources of Biopharmaceuticals
THERAPEUTIC PROTEINS: GROWTH FACTORS, HORMONES AND

CYTOKINES
Bioactivity of Expressed Proteins ‚ Synthesis and Secretion of Recombinant Proteins ‚
Xenotransplantation ‚ Discovery, Biological Roles and Properties of Naturally produced
Hormones: Insulin, Erythropoietin, Somatotropin and Interferons - Development of
Recombinant Human Insulin, Erythropoietin, Growth Hormone and Interferons
RECOMBINANT BLOOD PRODUCTS, THERAPEUTIC ENZYMES, VACCINES

AND ANTIBODIES
Introduction ‚ Haemostasis: Coagulation Pathway, Clotting Disorders and Blood-clotting
Factors ‚ Anticoagulants: Hirudin and antithrombin ‚ Thrombolytic agents: Tissue
Plasminogen Activator, Streptokinase, Urokinase and Albumin ‚ Therapeutic Enzymes:
Asparaginase, Urate Oxidase and Superoxide Dismutase ‚ Vaccines: Traditional Vaccine
Preparations, Recombinant Vaccines and Peptide Vaccines ‚ Adjuvant Technology ‚
Polyclonal and Monoclonal antibodies
NUCLEIC ACID- AND CELL-BASED THERAPEUTICS

Introduction - Basic approach to gene therapy - Retroviral, Adenoviral and non-Viral Vectors
‚ Gene Therapy and Genetic Disease ‚ Gene Therapy and Cancer ‚ Gene-based Vaccines ‚
Antisense Technology ‚ Cell- and Tissue-based Therapies: Stem Cells and Adult Stem Cells.
PRODUCT ANALYSIS
Introduction ‚ Protein-based Contaminants ‚ Product Potency and Determination of Protein
Concentration ‚ Detection of Protein-based Impurities ‚ Immunological Approaches to
Detection of Contaminants ‚ Endotoxin and Other Pyrogenic Contaminants ‚ Viral Assays ‚
Miscellaneous Contaminants
TEXT-BOOK
1. Gary W., Pharmaceutical Biotechnology: Concepts and Applications, John Wiley &
Sons Ltd., Sussex, England, 2007.
REFERENCES
1. S.S. Purohit, H.N. Kakrani and A.K. Saluja (2006) Pharmaceutical Biotechnology,
Jodhpur, India, Student Edition, 2006.
2. Kayser, O. and Müller R. H., Pharmaceutical Biotechnology: Drug Discovery
and Clinical Applications Wiley-VCH, 2004.
3. Dutton, R. and Scharer, J., Advanced Technologies in Biopharmaceutical Processing,
Blackwell Publishing, 2007.
L T P C
BIT 311 HEALTHCARE BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Differentiate simple proteins and valuable therapeutic proteins
CO2: Explain the production of various recombinant growth hormones
CO3: Describe production and applications of monoclonal antibodies and vaccines
CO4: Understand the mechanism involving in gene therapy
CO5: Discuss the use of antisense oligonucleotides in neurological disorders

CO1 H M H
CO2 H H M H M
CO3 H M H M M M
CO4 H M M M
CO5 M M M H M M
SIMPLE PROTEINS AND THERAPEUTIC AGENTS

Proteins as therapeutic agents - Choice of expression systems and optimizing gene expression
- Applications, delivery and targeting of therapeutic proteins ‚ Regulatory aspects of
therapeutic proteins
HORMONES, RECOMBINANT BLOOD PRODUCTS &ENZYMES AS

THERAPEUTIC AGENTS
Insulin, Glucagon, Human growth hormones - Gonadotrophins - Haemostasis ‚
Anticoagulants - Thrombolytic agents - Enzymes of therapeutic value - Asparaginase - Dnase
‚ Glucocerebrosidase ‚ Galactosidase - Urate oxidase ‚ Laronidase - Superoxide dismutase -
Debriding agents - Digestive aids
MONOCLONAL ANTIBODIES & VACCINES

Introduction to monoclonal antibodies - Development of monoclonal antibodies - Expression
of antibody molecules - Purification of monoclonal antibodies - Clinical uses of monoclonal
antibodies - Hybrid human - Mouse antibodies - Production of recombinant monoclonal
antibodies, Bacterial polysaccharides, proteins and toxins as vaccines - Recombinant
vaccines- subunit, attenuated and vector vaccines - Multivalent vaccine development against
AIDS
CYTOKINES & GENE THERAPY

Interferons- Engineering human interferons -Tumour necrosis factor ‚ interleukins -
Haemopoietic growth factors - Gene therapy ‚ in search of the perfect disease - Gene therapy
‚ the real diseases - Delivery systems for gene therapy - Gene therapy in the clinic
PEPTIDES & ANTISENSE OLIGONUCLEOTIDES

The nervous system- Immune responses to peptides - Neurological diseases - The use of
peptides in the treatment of neurological disease -The science of antisense - Requirements of
a genetic drug- Mechanisms of action of antisense molecules - Animal models and
oligonucleotides- Clinical trials- towards the next generation of antisense drugs
TEXT BOOKS
1. Ratledge, C., Kristiansen, B., Basic Biotechnology, Cambridge University Press,
USA, 2nd Edition, 2001.
2. Walsh, G., Pharmaceutical Biotechnology: Concepts and Applications, John Wiley &
Sons, England, 2007.
3. Brooks, G., Biotechnology in Healthcare: An introduction to biopharmaceuticals,
Pharmaceutical Press, London, 1998.
REFERENCE
1. David, E., Technology and Future of health care, Preparing for the Next 30 years,
Jhon Wiley, Singapore, 2nd Edition, 2000.
L T P C
BIT 313 METABOLIC ENGINEERING
3 0 0 3
Course Outcomes:
CO1: State the role of transport processes in metabolic pathways and material balance
CO2: Describe the regulation of enzymes involved in metabolic pathways
CO3: Employ various strategies to manipulate the production of industrially important
metabolites
CO4: Build algorithms for biosynthesis pathways
CO5: Explain metabolic flux analysis and its role in manipulation of metabolite production.

CO1 H M
CO2 H M
CO3 H M M M
CO4 M H H
CO5 M H H
REVIEW OF CELLULAR METABOLISM AND MATERIAL BALANCE

Transport processes; Active and Passive transport, Facilitated diffusion - Fueling reactions;
Glycolysis, TCA, fermentative pathways etc - Biosynthetic reactions; Biosynthesis of amino
acids, nucleic acids, fatty acids and other building blocks - Polymerization - Growth
energetic - Black Box model - Elemental balances and Heat balance
REGULATION OF METABOLIC PATHWAYS

Enzyme kinetics; Mechanisms and their dynamic representation - Regulation of enzyme
activity versus regulation of enzyme concentration - Regulation of metabolic networks -
Regulation of at the whole cell level - Regulation of metabolic networks - Example of
important pathways - Case studies and analytical type problem
METABOLIC ENGINEERING IN PRACTICE

Examples of pathway manipulations - Enhancement of product yield and productivity -
Extension of substrate range - Extension product spectrum and novel products - Improvement
of cellular properties - Xenobiotic degradation
METABOLIC PATHWAY SYNTHESIS

Metabolic pathway synthesis algorithm - Overview of the algorithm - Case studies; Lysine
biosynthesis etc. - Synthesis of alanine and serine synthesis
METABOLIC FLUX ANALYSIS

Metabolic flux analysis - Overdetermined systems - Underdetermined systems; Linear
Programming - Sensitivity analysis - Applications of metabolic flux analysis; aminoacid
production - Example: Glutamic acid production etc.
TEXT BOOK
1. Gregory N. Stephanopoulos, Aristos A. Aristidou., Metabolic engineering: Principles
and Methodologies, Jens Nielsen Academic Press, 1st Edition, 1998.
2. Christina D. Smolke., The Metabolic Pathway Engineering Handbook: Fundamentals,
CRC Press, New York, London, 1st Edition, 2010.
3. Cortassa S., Aon M.A., Iglesias A.A and Lioyd DAn Introduction to Metabolic and
Cellular Engineering, World Scientific Publishing Co., Singapore, 1st Edition, 2002.
4. Sang Yup Lee and Eleftherios T. Papoutsakis., Metabolic Engineering, CRC Press,
REFERENCES
1. Wang.D.I.C Cooney C.L., Demain A.L., Dunnil.P. Humphrey A.E. Lilly M.D.,
Fermentation and Enzyme Technology, John Wiley and sons, 1980.
2. Stanbury P.F and Whitaker A., Principles of Fermentation Technology, Pergamon
Press, 1984.
3. Zubay G., Biochemistry, Macmillan Publishers, 1989.
4. Gerhard Gottschalk., Bacterial Metabolism, Springer Verlag, 2nd Edition, 1986.
L T P C
BIT 314 DRUG DESIGN AND DEVELOPMENT
3 0 0 3
Course Outcomes:
CO1: Describe the stages in drug discovery and development
CO2: Depict the sources of drugs, screening of natural compounds and compound databases.
CO3: Explain about various drug targets and strategies for rational drug design.
CO4: Explain the use of computation in structure-based and Ligand-based drug design.
CO5: Describe the use of combinatorial chemistry to construct compound libraries and
screening them using various computational tools.

CO1 H M
CO2 H M
CO3 M H M
CO4 H M
CO5 H M
INTRODUCTION TO DRUG DISCOVERY AND DEVELOPMENT

Organized drug discovery, Drug Development, Clinical Trials, and Sources of Drugs-
Natural sources, Organic synthesis, Prokaryotic and Eukaryotic Cells as bio-factories,
Pharmacology- Basics of Pharmacokinetics and Pharmacodynamics.
APPROACHES TO NEW DRUG DISCOVERY

Rational basis of Drug Design, New approaches for lead identification- Using Disease
Models as Screens for New Drug leads, Developing bioassays for drug screening, Lead
optimization approaches- Conformation restriction, Pharmacophore, Metabolic stabilization.
ENZYMES AND RECEPTORS AS TARGETS OF DRUG DESIGN

Enzyme catalytic principles- Lock and Key and Induced fit theory, Types of enzyme
inhibition, Rational design of enzyme inhibitors, affinity labels, Suicide inhibitors, transition
state mimicry, Illustrative examples - ACE, renin and HIV protease inhibitors, Receptor
theory, Molecular Biology of receptors, Receptor Binding Assays, Lead Compound
Discovery of Receptor agonists and antagonists, Illustrative examples- Adenosine receptor
agonists and antagonists
COMPUTER AIDED DRUG DESIGN

Virtual Screening, CADD approaches, Structure based drug design: Molecular Docking ‚
Force field Calculation, Energy minimization, Scoring methods in docking, Ligand based
drug design: QSAR and Pharmacophore modeling. Overview of computer based tools for
drug designing - auto dock, GRAMM, CAMD.
CURRENT STATUS AND FUTURE PROSPECTS

Combinatorial chemistry, Combinatrial synthesis and compound libraries, peptidomimetics,
Peptide libraries- Peptide libraries through phage display; Applications in epitope and
mapping, synthetic vaccine design.
TEXT BOOKS
1. Larsen, P.K, Liljefors, T and Madsen U., Text Book of Drug Design and Discovery,
Taylor and Francis, London and Newyork, 3rd Edition, 2002.
2. Scolnick, E.M, Advances in Protein Chemistry, Vol 56, Drug Discovery and Design
Academic Press, London, 2001.
3. Walsh, G., Biopharmaceuticals-Biochemistry and Biotechnology, Wiley, Singapore,
2nd Edition, 2003.
REFERENCES
1. Perun, T. J. and Propst, C. L., Computer Aided Drug Design, Dekker, 1st Edition,
1989.
L T P C
BIT323 EXPLORING THE MICROBIAL WORLD
3 0 0 3
Course Outcomes:
CO1: Explain about the nutritional types of bacteria
CO2: Summarize the applications of microorganisms in petroleum augmentation and
recovery
CO3: Describe about the microbial production of food
CO4: Explain about biofertilizers and biopesticides

CO5: Summarize the applications of microbes in waste water treatment
CO1 H
CO2 H M H H
CO3 H M M H M
CO4 H H H M
CO5 H H H M M
INTRODUCTION
Microorganisms: types, morphology, ultra‚ structure and reproduction of bacteria, nutritional
requirements of microorganisms, nutritional types of bacteria, pure cultures and aseptic
techniques
MICROORGANISMS AS A SOURCE OF RENEWABLE ENERGY

Scope and importance Renewable sources, energy from waste materials, production of non-
conventional fuels ‚ methane (biogas), hydrogen and ethanol. Use of microorganisms in
petroleum augmentation and recovery; bio-diesel from microbial sources. Microbial fuel
cells.
MICROORGANISMS AS FOOD
Microbial production of food (SCP), Essential prerequisites for organisms to be used as SCP
& as food and feed supplements. Major groups of microorganisms used, relative advantages
and disadvantages. Microbial production of flavours and food colorants. Probiotics and
prebiotics.
BIOFERTILIZERS AND BIOPESTICIDES

Microbial Pesticides/Insecticides, Biocontrol of plant pathogens and mechanisms involved in
biocontrol - amensalism, competition, predation and parasitism, antibiosis and siderophore
production, Integrated pest management. Biofertilisers, biological nitrogen fixation and
phosphate solubilisation, VAM fungi and crop productivity
MICROBIAL TECHNOLOGY FOR WASTEWATER TREATMENT

Wastewater characteristics - Biological wastewater treatment - Unit operations and
processes; attached growth processes - trickling filters and rotating biological contactors,
Suspended growth processes - aerated lagoons, oxidation ponds and the activated sludge
process; anaerobic digestion.
TEXT BOOKS
1. Glazer, A.N. and Nikaido, Microbial Biotechnology, Freeman and company, 2nd
Edition, 2007.
REFERENCES
1. Young, M.Y., Comprehensive Biotechnology, Vol 1-4, Pergamon Press, Oxford, 1st
Edition, 1985.
2. Rittman, B and McCarty, P.L., Environmental Biotechnology: Principles and

Applications, McGraw- Hill, 2nd Edition, 2000.
L T P C
CHE 352 BIOPROCESS INSTRUMENTATION AND CONTROL
3 0 0 3
Course Outcomes:
CO1: Describe quantitatively the dynamic behavior of process systems
CO2: Describe different types of controllers and control strategies
CO3: Develop the ability to describe quantitatively the behavior of simple control systems
CO4: Develop the ability to design control systems
CO5: To tune a control loop and to apply this knowledge in measurements

CO1 H M
CO2 H M
CO3 H M
CO4 H M M
CO5 H M M
LINEAR OPEN LOOP SYSTEMS

- First order and linearized first order systems - Response to various disturbances - First order
in series- Higher order systems - Response to various disturbances
FEEDBACK CONTROL SYSTEMS

Controls - Block diagram - Closed loop transfer function - Transient response - Simple alarm
modes of control and controller characteristics- Servo and regulatory problems
STABILITY ANALYSIS
Stability - Routh analysis - Frequency response - Control system design - Controller tuning
ADVANCED CONTROL SYSTEMS

Cascade - Feed forward and ratio control - Dead time compensation - Internal Model Control
- Control valves
APPLICATION TO BIOLOGICAL SYSTEMS

Process modelling and control in pharmaceutical operations - Process modelling and control
for drug delivery ‚ Gene regulatory control ‚ Signal transduction networks
TEXT BOOKS
1. Seborg, D. E. and Mellichamp, D. A., Process Dynamics and Control, Wiley, New
York, 3rd Edition, 2010.
2. Coughnowr, D. P., Process Systems Analysis and Control, McGraw Hill, New York,
2nd Edition, 1991.
REFERENCES
1. Harriot, P., Process Control, Tata McGraw Hill, New Delhi, 4th Edition, 2005.
2. Smith, C. A. and Corripio, A. B., Principles and Practice of Automatic Process
Control, Wiley, New York, 2nd Edition, 1997.
TRANSPORT PHENOMENA IN BIOLOGICAL L T P C

CHE358
SYSTEMS 3 0 0 3
Course Outcomes:
CO1: Describe quantitatively the properties of fluids for momentum transport
CO2: Describe the dynamics of momentum transport
CO3: Develop the ability to describe quantitatively the behavior of energy transport
CO4: Develop the ability to describe quantitatively the behavior of mass transport
CO5: Develop the ability to describe quantitatively the behavior of oxygen transport in
biochemical systems

CO1 H M
CO2 H M
CO3 H M
CO4 H M
CO5 H M
INTRODUCTION TO TRANSPORT PROCESSES IN BIOLOGICAL SYSTEMS

Role of transport processes in biological systems ‚ definition of transport processes ‚
Relative importance of convection and diffusion ‚ Transport within cells ‚ Transcellular
transport ‚ Physiological transport systems ‚ Application of transport processes in disease
pathology, treatment and device development ‚ Relative importance of transport and reaction
processes
MOMENTUM TRANSPORT IN BIOLOGICAL SYSTEMS

Rheology and flow of blood ‚ Conservation of mass in 3-D ‚ Conservation of linear
momentum and Navier-Stokes equation ‚ Fluid motion with more than one independent
variable ‚ Dimensional analysis and Dimensionless groups
PHYSIOLOGICAL FLOW IN BIOLOGICAL SYSTEMS

Integral form of equation ‚ Bernoulis equation applied to Stenotic heart valves ‚ Boundary
layer theory ‚ Flow separation ‚ Lubrication theory ‚ peristaltic pumping
MASS TRANSPORT IN BIOLOGICAL SYSTEMS

Solute fluxes in mixtures ‚ Conservation relations ‚ Constitutive relations ‚ Diffusion as
random walk ‚ Estimation of diffusion coefficients in solution ‚ Steady state diffusion in one
dimension‚ unsteady state diffusion in one dimension ‚ Diffusion limited reactions ‚

Electrolyte transport ‚ Diffusion and convection
POROUS MEDIA AND TRANSVASCULAR TRANSPORT

Porosity, tortuosity and volume fraction ‚ Fluid flow in porous media ‚ Solute transport in
porous media ‚ Fluid transport in poroelastic materials ‚ Pathways for transendothelial
transport ‚ rates of transvascular transport ‚ Phenomenological constants in the analysis of
transvascular transport ‚ A limitation of Starlings law
TEXT BOOKS
1. Truskey, G.A., Yuan, F., David, F.K., Transport Phenomena in Biological Systems,
Prentice Hall, New Jersey, 2nd Edition, 2009.
REFERENCES
1. Bird, R.B., Stewart, W.E., Lightfoot, E.N., Transport Phenomena, Revised John
Wiley and sons, Singapore, 2nd Edition, 2007.
MAJOR ELECTIVES FOR IV YEAR
L T P C
BIT 405 NANOBIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Explain the characteristics of nanomaterials.
CO2: Identify the various nanodevices and nanostructures
CO3: Describe fabrication techniques.
CO4: Elucidate the applications of CNTs, fullerenes, quantum dots and gold nanoparticles
CO5: Suggest a suitable drug delivery nanodevice

CO1 M M
CO2 M H
CO3 M H
CO4 M M M H M
CO5 M M H M
INTRODUCTION - TECHNOLOGICAL IMPACT OF NANOSCALE SYSTEMS

Micro and nano systems and technologies - Overview of nanodevices and techniques
SYNTHESIS AND CHARACTERIZATIONS OF NANOSCALE MATERIALS

Strategies for nano architecture - Top down and bottom up approaches - Fabrication
technologies and characterizations - Self assembly systems - some aspects of nanofluidics-
Surfactants, polymers, emulsions and colloids.
INORGANIC NANOSCALE SYSTEMS FOR BIOSYSTEMS

Nano-structured materials ‚ Properties, characteristics and applications, fullerenes, carbon
nanotubes, quantum dots and wires, gold nanoparticles and nanopores
APPLICATIONS OF NANO-MOLECULES IN BIOSYSTEMS

Molecules of life - Proteins, lipids, RNA and DNA ‚ Nanoscale elements for delivery of
materials into cells, peptides coupled nanoparticles - DNA based artificial nanostructure
proteins as components in nanodevices
APPLICATION OF NANO-BIOTECHNOLOGY IN DRUG DELIVERY

Nanoscale devices for drug discovery Micelles for drug delivery protein targeting - Small
molecule - Protein interactions, microarray and genome chips
TEXT BOOKS
1. Wilson, M., Kannangra, K. and Smith, G., Nanotechnology, Oversees Press India
Private Ltd, NewDelhi, 2nd Edition, 2005.
2. Jain, K.K., Nanobiomolecular Diagnostics: Current Techniques and Application,

Taylor and Fransis Publishers, New York, 1st E dition, 2006.
REFERENCES
1. Nill, K., Glossary of Biotech and Nanobioterms, CRC Publisher, California, 4th
Edition, 2005.
L T P C
BIT 406 IPR IN BIOTECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Explain the establishment of Intellectual property Rights (IPR), including Patents,
Trademark and Copyright and other
CO2: Describe patent claim writing and analysis
CO3: Elaborate planning and documenting IPR development in organized research, using
modern ICT tools like MS project, MS Vision and Data Base Systems for IPR
maintenance
CO4: Explain development of Patent Search Strings and their use in Business Intelligence
work in reading and analyzing Law texts
CO5: Read and interpret state of the art scientific literature, and generate new ideas to tackle
technical problems in the area of biotechnology.

CO1 H M H H M H
CO2 H M H H
CO3 M H M H H
CO4 M H H H M H
CO5 M H H H
INTRODUCTION TO IPR
Invention and Creativity - Intellectual Property (IP), Importance - Protection of IPR - Basic
Types of Property (Movable, Immovable and Intellectual Property)
PATENTS IN IPR
Patents - Copyrights and related rights - Trade Marks and Rights Arising from Trademark
Registration - Definitions - Industrial Designs and Integrated Circuits - Protection of
Geographical Indications at National and International Levels - Application procedures,
PBR. IPR for Bioprocesses and Bioproducts
INTERNATIONAL CONVENTION RELATING TO INTELLECTUAL PROPERTY

Establishment of WIPO - Mission and activities - History - General agreement on Trade and
tariff (GATT). WIPO Copyright Treaty (WCT)
INDIAN POSITION VS WTO AND STRATEGIES & CASE STUDIES

Indian IPR Legislations - Commitments to WTO - Patent Ordinance and the Bill - draft of a
National Intellectual Property Policy - Case studies on - Patents (Basmati rice, Turmeric,
Neem, etc.) - Copyright and Related Rights - Trade Marks - Industrial Design and Integrated
Circuits - Geographic Indications - Protection against Unfair Competition
IPR TRANSACTIONS AND TECHNOLOGY TRANSFER CONTRACT

Various Types of Contracts - Assignment, Lease and License, Conditionality, Drafting of
Technology Transfer Contract and Global Sharing of Technology - Confidentiality and
Proper Use - Further Innovation and Invention - Ever-greening Chain
TEXT BOOKS
1. Carlos. C., Trade Related aspects of IPR, a Commentary on TRIPS agreement,
Oxford University Press, USA, 1st Edition, 2007.
2. Cornish, W.R., Intellectual Property- Patent, Copyright Trademarks and Allied rights,
Sweet and Maxwell, USA, 5th Revised Edition, 2003.
REFERENCES
1. Intellectual Property Today [www.iptoday.com].
2. Eli Whitney., United States Patent Number: 72X, Cotton Gin, March 14, 1794.
3. David, C., The Role of Intellectual Property Rights in Biotechnology Innovation,
Edward Elgar Publishing Limited, Cheltenham, UK, 2009.
4. Storz, U., Intellectual Property Issues ‚ Therapeutics, Vaccines and Molecular
Diagnostics, Springer, Heidelberg, Germany, 2012.
L T P C
BIT 407 BIOREACTOR DESIGN AND ANALYSIS
3 0 0 3
Course Outcomes:
CO1: Describe the kinetics of reactions
CO2: Design equations to determine the performance of ideal reactors
CO3: Create various models for describing non- ideal behavior of reactors
CO4: Analyze Interaction of heat and mass transfer in the microbial processes
CO5: Describe the Scale up criteria of bioreactors

CO1 H M
CO2 H M
CO3 H M
CO4 H M
CO5 H M
INTRODUCTION
General design information; Material and energy balance calculations; Process Flow
sheeting.
IDEAL REACTORS
Fed-Batch reactors, Enzyme catalyzed reactions in CSTRs, CSTR reactors with Recycle and
Wall growth, Ideal Plug- Flow Tubular reactor.
DESIGN OF BIOREACTOR
Vessel, agitation system materials, welds, finish, valves, piping and valves for biotechnology,
special requirements of utilities and cleaning of production plants. Physical and chemical
sensors for the medium and gases, online sensors for cell properties, off-line analytical
methods; Biosensors. Calculation for designing a bioreactor.
SELECTION OF BIOPROCESS EQUIPMENT (UPSTREAM AND DOWNSTREAM)

Specifications of bioprocess equipment; Mechanical design of reactors, heat transfer and
mass transfer equipment; Design considerations for maintaining sterility of process streams
and process equipment; Piping and instrumentation; Materials of construction for bioprocess
plants.
BIOREACTOR SCALE-UP
Scale up and scale down issues: Effect of scale on oxygenation, mixing, sterilization, pH,
temperature, inoculums development, nutrient availability and supply; Bioreactor scale-up
based on constant power consumption per volume, mixing time, impeller tip speed (shear),
mass transfer coefficients. Scale up of downstream processes: Adsorption (LUB method);
Chromatography (constant resolution etc.); Filtration (constant resistance etc.);
Centrifugation (equivalent times etc.); Extractors (geometry based rules). Scale-down related
aspects.
TEXT BOOK
1. Robert H. Perry and Don W. Green., Perry's Chemical Engineers' Handbook,
McGraw Hill Book Co., 8th Edition, 2008.
REFERENCES
1. Michael Shuler and Fikret Kargi, Bioprocess Engineering, 2nd Edition, Prentice Hall,
Englewood Cliffs, NJ, 2nd Edition, 2002.
2. Roger Harrison et al., Bioseparations Science and Engineering, Oxford University
Press,. 3rd Edition, 2010.
3. J. M. Coulson and J. F. Richardson, R.K.Sinnott., An introduction to Chemical
Engineering Design, Butterworth-Heinemann Ltd., UK, 5th Edition.
L T P C
B1T 409 CANCER BIOLOGY
3 0 0 3
Course Outcomes:
CO1: Understand the regulation and modulation of cell cycle in cancer by various signal
switches
CO2: Explain and compare various types of carcinogenesis and its metabolism
CO3: Describe the role of activation of kinases, identification of oncogenes, and how
telomerase induced cancer
CO4: Explain metastasis and its significant clinical markers for invasion and metastasis
CO5: Describe molecular tool for early diagnosis of cancer, different forms of cancer
chemotherapy and radiation therapy

CO1 H H M H M M M M M
CO2 H H M M M M M M
CO3 H M M M M M M M M
CO4 H M M M M M M - M M -
CO5 H H M M M M - - - M M M
FUNDAMENTALS OF CANCER BIOLOGY

Regulation of cell cycle, mutations that cause changes in signal molecules - Effects on
receptor, signal switches, tumour suppressor genes - Modulation of cell cycle in cancer,
different forms of cancers, diet and cancer
PRINCIPLES OF CARCINOGENESIS
Theory of carcinogenesis -Chemical carcinogenesis, metabolism of carcinogenesis, principles
of physical carcinogenesis, x-ray radiation - Mechanisms of radiation carcinogenesis
PRINCIPLES OF MOLECULAR CELL BIOLOGY OF CANCER

Signal targets and cancer, activation of kinases - Oncogenes, identification of oncogenes,
retroviruses and oncogenes - Detection of oncogenes, oncogenes and proto oncogene activity
- Growth factors related to transformation - Telomerases
PRINCIPLES OF CANCER METASTASIS
Clinical significances of invasion, heterogeneity of metastatic phenotype - Metastatic
cascade, basement membrane disruption - Three step theory of invasion, proteinases and
tumour cell invasion
NEW MOLECULES FOR CANCER THERAPY

Cancer screening and early detection, detection using biochemical assays, tumor markers -
Molecular tools for early diagnosis of cancer-Different forms of therapy - Chemotherapy,
radiation therapy, detection of cancers, prediction of aggressiveness of cancer, advances in

cancer detection, use of signal targets towards therapy of cancer - Gene therapy
TEXT BOOKS
1. Weinberg, R.A., The Biology of Cancer, Garland Science Taylor and Francis Group,
2. Kleinsmith. L.J., Principles of Cancer Biology, Pearson Education Inc., San
Francisco, CA, 1st Edition, 2006.
REFERENCE
1. DeVita Jr, V.T., Lawrence, T.S., Rosenberg, S.A., DePinho, R.A. and Weinberg,
R.A., DeVita, Hellman, and Rosenberg's Cancer: Principles and Practice of
Oncology, Lippincott Williams & Wilkins Philadelphia, PA, 9th Edition, 2011.
L T P C
BIT 410 BIOMEDICAL ENGINEERING
3 0 0 3
INTRODUCTION TO BIOMEDICAL ENGINEERING, HUMAN ANATOMY AND

PHYSIOLOGY
Evolution of the Modern Health Care System ‚ The Modern Health Care System ‚ What is
Biomedical Engineering? ‚ Roles of the Biomedical Engineer ‚ Recent Advances in
Biomedical Engineering ‚ Professional Status of Biomedical Engineering ‚ Professional
Societies ‚ Introduction to Anatomy and Physiology - Cellular Organization ‚ Tissues ‚
Major Organ Systems ‚ Homeostasis.
BIOMECHANICS, BIOMATERIALS AND TISSUE ENGINEERING

Introduction to Biomechanics ‚ Basic Mechanics ‚ Mechanics of Materials ‚ Viscoelastic
Properties ‚ Cartilage, Ligament, Tendon and Muscle ‚ Cardiovascular Dynamics ‚
Biomaterials: Types, Properties and Their Applications ‚ Tissue-Biomaterial Interactions ‚
Biomaterials Processing Techniques for Guiding Tissue Repair and Regeneration ‚
Application-specific Strategies for Design and Selection of Biomaterials ‚ Introduction to
Tissue Engineering ‚ Physical and Biological Considerations ‚ Scaling Up ‚ Implementation
of Tissue Engineered Products.
BIOINSTRUMENTATION, BIOMEDICAL SENSORS AND BIOSIGNAL

PROCESSING
Introduction ‚ Basic Bioinstrumentation System ‚ Fundamental Electrical Quantities ‚
Linear Network Analysis ‚ Linearity and Superposition ‚ Thevenin•s Theorem - General
Approach to Solving Circuits Involving Resistors, Capacitors and Inductors ‚ Operational
Amplifiers ‚ Time-Varying Signals ‚ Active Analog Filters ‚ Bioinstrumentation Design ‚
Introduction to Biomedical Sensors ‚ Biopotential Measurements ‚ Physical Measurements ‚
Blood gas, Bioanalytical and Optical sensors ‚ Physiological Origins of Biosignals ‚
Characteristics of Biosignals ‚ Signal Acquisition ‚ Frequency Domain Representation of
Biological Signals ‚ Linear Systems ‚ Signal Averaging.
BIOELECTRIC PHENOMENA AND BIOMEDICAL TRANSPORT PROCESSES
Introduction and History ‚ Neurons ‚ Basic Biophysics Tools and Relationships ‚ Equivalent
Circuit Model for Cell Membrane ‚ Model of Whole Neuron ‚ Chemical Synapses ‚
Biomedical Mass Transport ‚ Biofluid Mechanics and Momentum Transport ‚ Biomedical
Heat Transport.
IMAGING, BIOMEDICAL OPTICS AND LASERS

Introduction to Radiation Imaging ‚ Emission Imaging Systems ‚ Instrumentation and
Imaging Devices ‚ Radiographic Imaging Systems ‚ Introduction to Medical Imaging ‚
Diagnostic Ultrasound Imaging ‚ Magnetic Resonance Imaging ‚ Magnetoencephalography
‚ Contrast Agents - Comparison of Imaging Modes ‚ Image Fusion ‚ Introduction to
Essential Optical Principles ‚ Basics of Light Propagation in Biological Tissue ‚ Physical
Interaction of Light and Physical Sensing ‚ Biochemical Measurement Techniques Using
Light ‚ Photothermal Therapeutic Effects of Light Sources ‚ Fibre Optics and Waveguides in
Medicine ‚ Biomedical Optical Imaging.
TEXT BOOK
1. Enderle, J. and Bronzino, J., Introduction to Biomedical Engineering, Academic
Press, MA, USA, 3rd Edition, 2012.
REFERENCE
1. Bankman, N.I. et al., Biomedical Engineering Desk Reference, Academic Press, MA,
USA, 1st Edition, 2009.
L T P C
BIT 411 BIORESOURCE TECHNOLOGY
3 0 0 3
Course Outcomes:
CO1: Identify various renewable energy sources
CO2: Describe large- scale fuel technologies and bioconversions
CO3: Demonstrate how biogas is produced from various bio-resources
CO4: Distinguish between the processes involved in bioethanol and butanol production
CO5: Evaluate the mechanisms involved in biodiesel production

CO1 H H H H H M
CO2 H M H H
CO3 H H M H H H
CO4 H H M H H H
CO5 H H H M H H
RENEWABLE ENERGY SOURCE

Hydropower, geothermal power, solar power, wind power ‚ Biofuel -Biomass - Feed stocks
(agricultural crops, bioenergy crops, agricultural waste residues, wood residues, waste
stream)
FUEL TECHNOLOGY AND BIOCONVERSION

History - Definition of biofuel, applications of biofuel (transport, direct electricity generation,
home use and energy content of biofuel) - Bioconversion of lignocellulosics, cellulose
saccharification, pretreatment technologies (air separation process, mechanical size
reduction, autohydrolysis) - Pulping and bleaching ‚ Enzymatic deinking.
BIOGAS
Biogas plant, feed stock materials, biogas production, factors affecting methane formation -
Role of methanogens ‚ Biohydrogen production - Oxygen sensitivity problems in
hydrogenenases
BIO ETHANOL AND BUTANOL

Advantages of ethanol over fossil fuels, production of ethanol from cellulosic materials,
ethanol recovery - Biobutanol production, energy content and effects on fuel economy -
Octane rating, air fuel ratio, specific energy, viscosity, heat of vaporization -Butanol fuel
mixtures
BIODIESEL
Production of biodiesel, oil extraction from algae by chemical solvents, enzymatic, expeller
press - Osmotic shock and ultrasonic assisted extraction - Applications of biodiesel,
environmental benefits and concerns
TEXT BOOKS
1. Alain A.V., Biomass to biofuels strategies for global Industries, John Wiley & sons
ltd, 1st Edition, 2010.
2. Twidell., J & Weir., T., Renewable energy resources, Taylor & Francis 2nd Edition,
2006.
REFERENCE
1. Luque, R., Camp, J., Hand book of biofuel production processes and technologies,
Woodhead publishing ltd., 1st Edition, 2011.
L T P C
BIT 412 RNAi TECHNOLOGY
3 0 0 3
Course Outcomes:
CO 1: Rationlize the discovery of gene silencing phenomenon
CO 2: Analyze the award of Nobel prize for the discovery of RNA interference
CO 3: Design vectors to initiate gene silencing
CO4: Report a method to avoid off-target gene silencing and its importance
CO5: Identify ways of applying RNA interference in Agriculture and Healthcare
CO 1 H H M M M M M
CO 2 M M H M M M
CO 3 H M M H M M M M
CO4 H M H M M M M
CO5 H H M M M M M M M
INTRODUCTION TO RNAi TECHNOLOGY

Introduction to Gene silencing ‚ Different names of gene silencing, Antisense RNA
technology -Discovery of gene silencing in petunia plants ‚ Sense co-suppression in plants
and animals- Discovery in nematodes (C. elegans) and award of Nobel Prize - Biochemistry
of RNAi.
METHYLATION, TRANSCRIPTIONAL AND SYSTEMIC SILENCING

Methylation of DNA and proteins, Viroid infection in plants and methylation of DNA,
Epigenetics, RNA directed DNA methylation (RdDM) - Proposed model of RdDM ‚
Transcriptional gene silencing and methylation, Induction of transcriptional gene silencing,
systemic silencing in plants and animals.
TECHNIQUES IN RNAi TECHNOLOGY

Particle bombardment method - Stable transformation of plants and animals by RNAi
vectors, agroinfiltration, VIGS methodology in plants and animals, selection of siRNA
sequence, delivery of siRNA using nanoparticles, transfection of siRNA Duplexes -Protein
knock down detection by western blotting and immunofluorescence.
Micro RNA
Biosynthesis of micro RNA, Differences between siRNA and miRNA, - Mechanism of
micro-RNA mediated interference, Artificial micro-RNA, Micro RNA in plants and animals.
APPLICATIONS OF RNAi TECHNOLOGY

RNAi for crop improvement ‚ Functional genomics in plants by gene silencing ‚ RNAi to
control plant viruses ‚ Viral encoded silencing suppressors and its applications ‚ RNAi to
control cancer ‚ miRNA directed control of cancer
TEXT BOOKS
1. Gaur R.K., Gafni Y, Sharma P, Gupta V.K., RNAi Technology. CRC Press, New
York, 2011.
2. Gregory, J., Hannon., RNAi- A Guide to Gene Silencing, Cold spring harbor
laboratory Press New York, 2003.
3. Latterich M., RNAi Advanced Methods, Taylor and Francis, New York, 2007.
L T P C
BIT 413 VACCINOLOGY
3 0 0 3
Course Outcomes:
CO1: Explain the history and background of most important vaccines
CO2: Distinguish various types of vaccines and guidelines for current vaccine practices
CO3: Identify the challenges in the development, testing and approval of new vaccines
CO4: Defend the ethics surrounding the use of vaccines
CO5: Assess the advantages and disadvantages of current vaccines critically

CO1 H H M M M M
CO2 H M M H M H H H
CO3 H M H H M M H H
CO4 M M M H
CO5 M H H M H H H
INTRODUCTION TO IMMUNITY
Innate Immunity; Activation of the Innate Immunity through TLR mediated signaling;
Adaptive Immunity; T and B cells in adaptive immunity; Immune response in infection;
Protective immune response in bacterial; Viral and parasitic infections; Correlates of
protection
IMMUNE RESPONSE
Vaccination and immune response; Appropriate and inappropriate immune response during
infection: CD4+ and CD8+ memory T cells; Memory B cells; Generation and Maintenance
of memory T and B cells; Dendritic cells in immune response
VACCINATION
Adjuvants in Vaccination; Induction of Th1 and Th2 responses by using appropriate
adjuvants; Microbial, Liposomal and Microparticles as adjuvant; Chemokines and cytokines;
Role of soluble mediators in vaccination; Oral immunization and mucosal Immunity
VACCINES
Conventional vaccines; Bacterial vaccines; Live attenuated and inactivated vaccine; Subunit
Vaccines and Toxoids; Peptide Vaccine
NEW VACCINE TECHNOLOGIES

Rationally designed Vaccines; DNA Vaccination; Mucosal vaccination; New approaches for
vaccine delivery; Engineering virus vectors for vaccination; Vaccines for specific targets;
Tuberculosis Vaccine; Malaria Vaccine; HIV
vaccine

TEXT BOOKS
1. Edited by Stefan H.E. Kaufmann, Novel Vaccination Strategies, Wiley-VCH Verlag
GmbH & Co. KgaA, 2004.
REFERENCES
1. Topley & Wilson•s, Microbiology and Microbial Infections Immunology Edited by
Stefan H.E. Kaufmann and Michael W Steward Holder Arnold, ASM Press, 2005.
2. Charles A Janeway. Jr, Paul Travers, Mark Walport and Mark J Shlomchik, Immuno
Biology, The Immune system in health and Disease, Garland Science, New York, 6th
Edition , 2005.
L T P C
BIT417 BIOSENSORS
3 0 0 3
Course Outcomes:
CO1:Understand the basic concepts of biosensors
CO2:Compare various types of biosensors
CO3:Apply immobilization techniques
CO4:Analyze multi analytes
CO5:Illustrate various applications of biosensors

CO1 H M H
CO2 H M M M
CO3 H M H M
CO4 H H H H H
CO5 H H H H H
INTRODUCTION TO BIOSENSORS
Definitions, , types of sensors, target analytes, various recognition, signals, and device types,
history of field - course overview, class survey, definitions, motivation, biological
inspiration.
RECOGNITION / TRANSDUCTION
Enzyme sensors- affinity sensors: antibodies, oligo-nucleotides measuring binding in affinity
sensors, SPR, quartz crystal microbalance, FRET- membrane protein sensors: ion channels,
receptor - whole cell sensors ‚ bacteria, yeast, mammalian cells
IMMOBILIZATION
Immobilization: adsorption, encapsulation - (hydro-gel, sol-gel glass, etc.), covalent
attachment, diffusion issues - optical fiber sensors, planar wave-guides

DEVICE INTEGRATION
Micro-scale and nanoscale: BioMEMS, nanowires, quantum dots, magnetic beads, PEBBLE
sensors measuring complex samples, multi-analyte detection, continuous measurements,
reagentless biosensors
APPLICATIONS
Agricultural, food safety, food processing : state of the field, market potential, unique design
criteria and needs, current sensors in use biomedical applications, bio-security,
environmental : state of the field, market potential, unique design criteria and needs, current
sensors in use
TEXT BOOKS
1. Bilitewski, U. and Turner, A.P.F., Biosensors for Environmental Monitoring.
Harwood Academic Publishers, The Netherlands. ISBN: 90-5702-449-7, 2000.
2. Ligler, F.S. and Rowe Taitt, C.A., Optical Biosensors: Present & Future. Elsevier,
The Netherlands. ISBN: 0-444-50974-7, 2002.
REFERENCE
1. Yang, V.C. and T.T. Ngo., Biosensors and Their Applications. Kluwer
Academic/Plenum Publishers, New York, NY. ISBN: 0-306-46087-4, 2000.
L T P C
BIT418 MOLECULAR PATHOGENESIS
3 0 0 3
Course Outcomes:
CO1: Understand the genetics and molecular biology of microbial pathogenesis.
CO2: Describe the clinical importance of host defense mechanisms and explain the
mechanisms of humoral and cellular defense.
CO3: Explain the role of microbial virulence factors in host- pathogen interactions.
CO4: Describe the methods used for studying host pathogen interactions.
CO5: Illustrate the classical and new therapeutic strategies for diagnosing and controlling
microbial pathogens.

CO1 H M H
CO2 M M M H
CO3 M H
CO4 M M H
CO5 M H
INTRODUCTION:
Historical perspective - Pasteur•s contributions, Koch•s postulates, early discoveries of

microbial toxins and antibiotics - Introduction to pathogenesis, components of microbial

pathogenicity - Population genetics of microbial pathogenesis ‚ epidemiology - various types
of pathogens and modes of entry ‚ attributes and components of pathogenicity
HOST-DEFENSE MECHANISMS
Host defense against pathogens, clinical importance of understanding host defense,
components of the host surface defences systems like skin, mucosa, eye, mouth, respiratory
tract, physical movements, limitation of free iron, antimicrobial compounds - mechanism of
killing by humoral and cellular defense mechanisms, complements, inflammation process,
general disease symptoms, Pathogenic adaptations to overcome the above defenses.
HOST-PATHOGEN INTERACTION:
Virulence and virulence factors, colonising virulence factors, virulence factors damaging the
host tissues, virulence genes and regulation of the virulence genes
Case studies: Diphtheria disease by colonisation; Disease without colonization: Clostridium
botulinum and Staphylococcus aureus; Intestinal infections: Shigella and E. coli infections;
Vibrio cholera Salmonella infections; fungal infections
EXPERIMENTAL METHODS TO STUDY HOST PATHOGEN INTERACTIONS:

Experimental methods to study host-pathogen interaction, selecting the pathogen model,
measurement of virulence - virulence assays: adherence, invasion, cytopathic, cytotoxic
effects identification of potential virulence factors; molecular characterization of virulence
factors, attenuated mutants, signal transduction & host response
.
DIAGNOSIS AND CONTROL OF PATHOGENS:
Classical approaches based on serotyping; modern diagnosis ‚ highly conserved virulence
factors, immuno & DNA-based techniques - New therapeutic strategies ‚ novel drugs for
new targets, vaccines - DNA, subunit and cocktail vaccines.
TEXTBOOKS
1. Groisman, E.A., Principles of Bacterial Pathogenesis, Academic Press, 2001.
2. Janeway C.A. Jr, and Travers P. T., Immunobiology, Blackwell J Scientific
Publishers, 1994.
REFERENCES
1. Iglewski B.H. and Clark V.L., Molecular basis of Bacterial pathogenesis, Academic
press, 1990.
2. Williams, P., Ketley, J. and Salmond, G., Methods in Microbiology: Bacterial
Pathogenesis, Vol. 27, Academic Press, 1998.
3. Salyers, A.A. and Whitt, D.D., Bacterial Pathogenesis ‚ A molecular Approach, ASM
Press, Washington, 2nd Edition , 2002
4. Nester, Anderson, Roberts, Pearsall, Nester., Microbiology: A Human Perspective,
McGraw-Hill, 3rd Edition, 2001.
5. Recent reviews in Infect. Immun. Mol. Microbiol, Biochem. J., EMBO etc.

L T P C
BIT419 MOLECULAR DIAGNOSTICS AND THERAPEUTICS
3 0 0 3
Course Outcomes:
CO1: Explain the molecular techniques for the analysis of Genetic and Neurological
disorders.
CO2: Discuss current methods of diagnosis for specific diseases, like tuberculosis
CO3: Recognize the importance of monoclonal and polyclonal antibodies in diagnosis
CO4: Apply genetic engineering tools in disease diagnosis.
CO5: Discuss current methods used for production of recombinant protein.

CO1 H H H M
CO2 H M H M M
CO3 M H H H M M
CO4 M M H H M H
CO5 M H M M
MOLECULAR DIAGNOSIS OF DISORDERS

Biochemical disorders; Immune, Genetic and Neurological disorders; Molecular techniques
for analysis of these disorders; Assays for the Diagnosis of inherited diseases; Bioinformatic
tools for molecular diagnosis
PROTEINS IN DIAGNOSTIC TECHNIQUES

Isolation of proteins and other molecules associated with disease; Process and their profiling
for diagnosis; 2D analysis of such proteins by sequencing individual spots by Mass
Spectrometry; Protein Micro array; Present methods for diagnosis of Specific diseases like
Tuberculosis, Malaria and AIDS; Ethics in Molecular Diagnosis
ANTIBODY BASED DIAGNOSIS

Monoclonal antibodies as diagnostic reagents; Production of monoclonal antibodies with
potential for diagnosis; Diagnosis of bacterial, viral and parasitic diseases by using; ELISA
and Western blot.
THERAPEUTIC AGENTS
Proteins as therapeutic agents - Choice of expression systems and optimizing gene expression
- Applications, delivery and targeting of therapeutic proteins - Engineering human interferons
and human growth hormones - Regulatory aspects of therapeutic proteins - Enzymes as
therapeutic agents - Use of genetically engineered DNase I and alginate lyase for treatment of
Cystic Fibrosis

VACCINES
Bacterial polysaccharides, proteins and toxins as vaccines - Recombinant vaccines- subunit,
attenuated and vector vaccines - Multivalent vaccine development against AIDS -
Commercial and regulatory aspects of vaccine production and its distribution-Production of
Recombinant Proteins having therapeutic and diagnostic applications, recombinant vaccine
TEXT BOOKS
1. Campbell, M.A and Heyer L.J., Discovering Genomics, Proteomics and
Bioinformatics, CSHL Press, Pearson/Benzamin Cummings San Francisco, USA, 2nd
Edition, 2007.
REFERENCES
1. Andrew Read and Dian Donnai, New Clinical Genetics, Scion Publishing Ltd,
Oxfordshire, UK, 2007.
2. James W Goding., Monoclonal antibodies: Principles and Practice, Academic Press,
3rd Edition , 1996.
L T P C
BIT420 SIGNAL TRANSDUCTION
3 0 0 3
Course Outcomes:
CO1: List the key regulatory mechanism of messengers and G protein coupled receptors role
in transport and cell cycle regulation
CO2: Demonstrate structure, function and modulation of different receptors and its cross talk
with in the cell communication
CO3: Explain and illustrate how the alteration of cellular signaling through either induction
or inhibition of transcription factors, phosphorylation and mrystoilation
CO4: Discuss molecular mechanism of signals in microbes and plants through regulation of
defense and elicitors and root nodule in rhizobium
CO5: Explain importance and various classifications of Ras proteins and its significance of
housekeeping function in cells

CO1 H - - H - H - - - H - -
CO2 H - - M - M - M - - - -
CO3 H - - H - M - H - M M M
CO4 H H - M H M - - - M - M
CO5 H - - M - M - M - M - M
MOLECULAR MECHANISMS OF SIGNAL TRANSDUCTION

Introduction - basic elements of Cell Signaling Systems - sensors, primary and secondary
messengers, calcium signaling, signaling Molecules and Cell-Surface Receptors Intracellular
Signal Transduction, G Protein‚ Coupled Receptors, G Protein‚ Coupled Receptors That
Regulate Ion Channels, Phospholipase C, Activation of Gene Transcription by G Protein‚

Coupled Receptors, Regulation of the Cell Cycle by Protein Kinases, Oncogenes, Tumor
Suppressor Genes, and Programmed Cell Death,
STRUCTURE AND FUNCTION OF SIGNALING PATHWAY

TGF Receptors and the Direct Activation of Smads, Cytokine Receptors and the JAK-
STAT Pathway, Receptor Tyrosine Kinases and Activation of Ras, MAP Kinase Pathways,
Phosphoinositides as Signal Transducers, Pathways That Involve Signal-Induced Protein
Cleavage, Down-Modulation of Receptor Signaling, Cross-Talk Among Different Signaling,
Pathways, the Role of NO as an Intercellular Messenger
SIGNALS AND GENE CONTROL

Experimental approaches for building a comprehensive view of signal- induced responses,
responses of cells to environmental Influences, control of cell fates by graded Amounts of
regulators, boundary creation by different combinations of transcription factors, extracellular
signals, reciprocal induction and lateral Inhibition, integrating and Controlling Signals,
signaling in regulation of protein phosphorylation, mrystioylation
SIGNALING IN MICROORGANISMS AND PLANTS

Molecular mechanism of ethylene signaling, light signal transduction in higher plant, LRR-
containing receptors regulating plant development, signaling in plant defense response like
PR protein family, salicylic acid, methyl jasmonate, super oxide anion, auxin action
signaling, signaling in transport and the control of plant growth and development, the roll of
signaling in formation of root nodule in rhizobium,
SIGNAL TRANSMISSION VIA RAS PROTEINS

General Importance and Classification of Ras Proteins, Membrane Localization of Ras
Protein, GTPase-activating Protein (GAP) in Ras Signal Transduction, Structure of Ras-GAP
Protein, Function of Ras-GAP Protein, Guanine Nucleotide Exchange Factors (GEFs) in
Signal Transduction via Ras Proteins, Raf Kinase as an Effector of Signal Transduction by
Ras Proteins, Structure of Raf Kinase, Interaction of Raf Kinase with Ras Protein,
Mechanism of Activation and Regulation of Raf Kinase, Reception and Transmission of
Multiple Signals by Ras Protein
TEXT BOOKS
1. B. D. Gomperts, Peter E. R. Tatham, Ijsbrand M. Kramer, Signal transduction,
Academic Press, 2nd Edition, 2009.
2. Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and
Peter Walter., Molecular Biology of the Cell, Garland Science;. New York, 5th
Edition, 2007.
3. Harvey Lodish, Arnold Berk, Chris A. Kaiser and Monty Krieger, Molecular Cell
Biology, W. H. Freeman and company, New York, 5th Edition, 2007.

L T P C
BIT421 FUNCTIONAL GENOMICS
3 0 0 3
Course Outcomes:
CO1: Summarize genome organization, sequencing, annotation and prediction of gene
structure in both prokaryotes and eukaryotes
CO2: Explain structure and function of genome based on sequence comparison, structural
analysis, functional and comparative genomics
CO3: Evaluation of pharmacogenomics and toxicogenomics through personalized medicine,
alteration of gene expression by drug, SNP marker for drug response, metabolism of
xenobiotics and predictive toxicogenomics
CO4: Describe personalized nutrition and disease prevention and its therapeutic applications
and epigenome analysis
CO5: Analyze effect of chemicals on gene changes such as mutation, DNA intercalation, and
chemogene in drug discovery

CO1 H - - H - H - - - H - -
CO2 H - - M - M - M - - - -
CO3 H - - H - M - H - M M M
CO4 H H - M H M - - - M - M
CO5 H - - M - M - M - M - M
ORGANIZATION AND SEQUENCING OF GENOME

Genome organization (prokaryotes and Eukaryotes) - Whole genome sequencing - Large
scale genome sequencing strategies - Genome sequence annotation - Genome assembly and
annotation - Prediction of Genes - ORF and functional prediction; Promoters, splice sites -
Regulatory regions ‚ Analysis of gene expression- Basic principles of DNA microarrays and
SAGE
STRUCTURAL AND FUCNTIONAL GENOMICS

Basic principles, approaches for target selection - application of sequence based and
structure-based approaches to assignment of gene functions, e.g. sequence comparison,
structure analysis (especially active sites, binding sites) and comparison, pattern
identification, etc. - Use of various derived databases in function assignment - Functional
genomics and Comparative genomics, Metagenomics.
PHARMACOGENOMICS AND TOXICOGENOMICS

Pharmacogenetic difference between populations, concept of personalized medicine ,Gene-
drug interactions, Drugs affecting gene expression, Biomarkers of drug response, SNPs as
markers of drug response, Pharmacogenomics of human p-Glycoprotein. Introduction to

toxicology, Absorption, distribution and excretion of toxicants, Metabolism of xenobiotics,

Gene Expression profiling in toxicology, Predictive toxicogenomics.
NUTRIGENOMICS
Induvidual differences and nutrition, personalized nutrition and disease prevention,
Personalized nutrition and therapeutic applications, Nutrigenomics of protein intake and
carbohydrate intake, Genetic association studies of coffee intake, Nutritional effect on
epigenome
CHEMOGENOMICS
Definition, Effect of chemicals on genes, delayed mutations, Interaction of molecules (small
& Big) with DNA intercalation, Cofactor Chemogenomics, Chemogenomics in drug
discovery.
TEXT BOOKS
1. Primrose, S. B., Twyman, R. M., Principles of Genome Analysis, Blackwell
Publishing, Singapore, 3rd Edition, 2002.
REFERENCES
1. Linco, J and Wong, M.L. (Eds)., Pharmacogenomics: The search for individualized
therapies, WILEY-VCH verlag Gmbh, Weinheim , Germany, 2002.
2. Innocenti, F., Pharmacogenomics: Methods and Protocols, Humana Press, New
Jersey, 1st Edition, 2005.
3. Hamadeh, H.K. and Afshari, C.A. (Eds.)., Toxicogenomics; Principles and
applications, John Wiley and sons, Canada, 2004.
4. Burczynski, M. E. (Ed.)., An Introduction to Toxicogenomics, CRC press, Florida,
2003
5. Bouchard, C. and Ordovas, J.M., Recent Advances in Nutrigenetics and
Nutrigenomics, Academic Press, USA, 2012.
6. Edgar, J. (Ed.)., Chemogenomics: Methods and Applications, Humana Press, New
Jersey, 2009.
L T P C
BIT422 RADIATION BIOLOGY
3 0 0 3
Course Outcomes:
CO1: Describe the concept involving in radiation biology
CO2: Differentiate the molecular and cellular effects of radiation
CO3: Understand the mechanisms of radiation protection
CO4: Explain the basic concepts in radiation oncology
CO5: Know the application of radiotherapy


CO1 H H M
CO2 H H H
CO3 M M H
CO4 M H M
CO5 M M M
INTRODUCTION TO RADIATION BIOLOGY

Physics and chemistry of radiation interactions with matter ‚ source and Types of ionizing
radiation - Particulate radiations - Linear Energy Transfer and Relative Biologic
Effectiveness- Radiation dose and units - Principles of radiation dosimetry - Direct and
indirect effects.
MOLECULAR AND CELLULAR RADIOBIOLOGY

Radiation lesions in DNA - DNA Strand Breaks and Chromosomal Aberrations - Cell
Survival Curves ‚ Radio-sensitivity and Cell Age in the Mitotic Cycle - Consequences of
unrepaired DNA damage: chromosome damage - Repair of Radiation Damage and the Dose
Rate Effect - Relative biological effectiveness (RBE) - Cellular repair exemplified in survival
curves - Cellular hyper-radiosensitivity (HRS) and induced repair (IRR) - Other molecular
targets: bystander (epigenetic) effects ‚ Molecular techniques.
RADIOBIOLOGICAL BASIS OF RADIATION PROTECTION

Radiation accidents and environmental radiation exposure - Long term radiation risks from
low radiation doses - Diagnosis and medical management of radiation syndromes - Radiation
carcinogenesis - Heritable radiation effects - Effects on the developing embryo - system for
radiation protection
RADIATION ONCOLOGY
Cell signaling - oncogenes and tumor suppressor genes - The cell cycle - Tumor growth and
cell kinetics - Cell death mechanisms - In vitro and in vivo assays for cell survival - Repair of
radiation damage - Tumor biology and host/tumor interactions - Radiobiology of normal
tissue damage - Time-dose-fractionation - Predictive assays
RADIOTHERAPY
Doses and Risks in Diagnostic Radiology, Interventional Radiology and Cardiology and
Nuclear Medicine - Clinical Response of Normal Tissues - Model Tumor System - Cell,
Tissue, and Tumor Kinetics - Time, dose, and fractionation in Radiotherapy- Combined
radiation and drug treatments - Clinical radiobiology of common cancers - Second cancers in
radiotherapy patients.
TEXT BOOKS
1. Radiation biology: A handbook for teachers and students, Training course series no.
42, International Atomic Energy Agency (IAEA), Vienna, 2010.
2. Eric J. Hall and Amato J. Giaccia., Radiobiology for the Radiologist, Lippincott,
Williams and Wilkins, 6th Edition, 2006.
REFERENCE
1. A.H.W. Nias, An Introduction to Radiobiology, Second Edition, John Wiley and
Sons, 1998.
L T P C
BIT423 RECOMBINANT PROTEIN PRODUCTION
3 0 0 3
Course Outcomes:
CO1: Understand structure of proteins, motifs, protein folding and posttranslational
modification of proteins
CO2: Explain and illustrate cloning of a gene in expression vectors of both bacterial and
mammalian expression vectors
CO3: Illustrate the overexpression and purification of proteins form both bacterial and
mammalian system by using His-tag, T7 and GST fusion tag
CO4: Explain the identification of differentially expressed proteins from infected tissues
through proteomics approaches such as 2D gel followed by LC-MS/MS analysis
CO5: Describe the commercial applications of recombinant proteins in pharmaceutical,
Immunological, and biosensor

CO1 H - - - - M - - - M - -
CO2 H - - - - M - M H H M H
CO3 H - - - - M M H H H M -
CO4 H - - - - M - H H H - -
CO5 H M - - M M M - M M - -
STRUCTURE OF PROTEINS AND POSTTRANSLATIONAL MODIFICATIONS

Introduction to primary, secondary, tertiary, quaternary and loop structure of proteins, motifs,
protein folding and denaturation. Post-translational modifications: acetylation,
phosphorylation, glycosylation, palmitoylation, mristoylation, sumolation and methylation.
Transcription factors.
TYPES OF EXPRESSION VECTORS AND CLONING

Major elements in the expression vectors: promoter, terminator, enhancer, fluorescent and
non fluorescent tags. Prokaryotic and eukaryotic expression vectors: pET, Gateway, pcDNA
3.1 expression system. Cloning of a gene: designing gene specific primers - PCR cloning.
OVER EXPRESSION AND PURIFICATION OF PROTEINS

Over expression of proteins in bacterial system, induction of †lac• promoter by IPTG, over
expression of proteins using mammalian expression vectors with fusion proteins: Priniciple
of Purification of protein using affinity chromatography (His-Taq, T7-tag and GST ‚ fusing
tag), ion exchange, gel filtration and FPLC.

PROTEOMIC APPROACHES
Introduction to proteomics, differential expression of proteins in healthy and infected tissues,
protein isolation from tissues, two dimensional gel electrophoresis, mass spectrophotometer
analysis, protein array and its applications.
APPLICATIONS OF RECOMBINANT PROTEINS

Raising of antibodies against recombinant proteins, antibody purification and immuno-
localization in tissues - generation of recombinant vaccines and purifications - different
applications of recombinant enzymes: industrial, environmental, therapeutic and biosensor
TEXT BOOK
1. Primrose, B., Twyman, R. M., Genomics: Applications in Human Biology, Blackwell
Publishing, Singapore, 4th Edition, 2004.
REFERENCE
1. Westermeier, R., Naven, T., Proteomics in Practice (A Laboratory Manual of
Proteome Analysis), Wiley-VCH, London, 3rd Edition, 2002.
L T P C
BIT424 CLINICAL TRIALS AND MANAGEMENT
3 0 0 3
Course Outcomes:
CO1: Understand the concept of new drug development process
CO2: Explain about good clinical practice and regulations
CO3: Know about feasibility and activity studies
CO4: Describe about clinical trial data
CO5: Know about international clinical trials

CO1 H M H
CO2 M M
CO3 M M M
CO4 M M M
CO5 M M H
DEVELOPING NEW DRUGS, BIOLOGICS, AND DEVICES

The Drug Development Process - Pre-Clinical Studies - Clinical Trial Phases - Application to
Market New Drugs and Biologics - FDA Review Groups - Developing New Devices - Post

marketing Surveillance of Drugs, Biologics, and Devices - Direct Reporting Based on

Observations
GOOD CLINICAL PRACTICE AND THE REGULATIONS

Guidelines - Local Laws - Principal Investigator Responsibilities - Sponsor Responsibilities -
Sponsor-Investigators - FDA Guidance Documents - Online Resources - Informed Consent
and the Regulations - Institutional Review Boards - Monitoring, Audits, and Inspections
PROTOCOL, FEASIBILITY AND ACTIVITY STUDIES, AND DOCUMENTATION

Common Components of a Protocol - Study Organization - Objectives/Endpoints - Study
Design - Study Population and treatment plan - Safety Assessment, Management, and
Reporting - Statistical Aspects - Subject Data and Record Retention ‚ Monitoring -
Reviewing a Specific Protocol - Study Start-up Phase - Study Maintenance Phase - Study
Completion and Close-Out Phase - Documents at Study Start-Up - Documents While the
Study is in Progress - Documents at Study Close-out - Maintaining Site Study File-
Management of Study Drugs, Biologics, and Devices
MANAGING CLINICAL TRIAL DATA

HIPAA, the Privacy Rule, and Clinical Trial Data - Guidelines and Regulations Regarding
Clinical Trial Data - Study Site Responsibilities Regarding Clinical Trial Data - Source
Document Verification of Clinical Trial Data - Release of Protected Medical Information -
Confidentiality of Clinical Trial Data - Endpoint Adjudication
GLOBAL HEALTH AND INTERNATIONAL TRIALS

International Clinical Trials - Ethnic and Racial Differences - Ethical Issues and Cultural
Sensitivities - Importance of International Trials - HIV/AIDS ‚ Malaria ‚ Tuberculosis ‚
Polio - International Regulations - Future Efforts
TEXT BOOK
1. Liu, M.B. and Davis, K., Clinical trials manual from the Duke Clinical Research
Institute: lessons from a horse named Jim., John Wiley & Sons, Ltd., 2nd Edition, 2010.
REFERENCE
1. Gallin, J.I. and Ognibene, F.P. Principles and Practice of Clinical Research,
Academic Press.

MINOR ELECTIVES
L T P C
CHE 354 MASS TRANSFER
3 0 0 3
Course Outcomes:
CO1: Solve diffusion and diffusion related problems
CO2: Estimate mass transfer coefficients for gas ‚ liquid contacting systems
CO3: Estimate the number of stages for distillation and absorption column
CO4: Solve problems related to extraction, leaching, adsorption and drying
CO5: Explain about membrane separation operations

CO1 H M M
CO2 H M M
CO3 H M M
CO4 H M M
CO5 H M
DIFFUSION
Diffusion, Equation of continuity - Unimolecular diffusion and equimolar counter diffusion
applied to both gases and liquids - Diffusion in solids, Knudsen diffusion, measurement of
diffusivity of liquids and gases - Empirical relations for measurement of diffusivity of gases
and liquids
INTERPHASE MASS TRANSFER

Mass transfer coefficients (k type and f-type), overall and local mass transfer coefficients,
Lewis Whitman two phase theory, estimation of mass transfer coefficients, analogy between
transport processes - Theories of mass transfer - Gas liquid contacting devices - Convective
mass transfer
VAPOR € LIQUID & GAS € LIQUID OPERATIONS

Vapor liquid equilibria, T-x-y and P-x-y diagrams, steam distillation, flashing, differential
distillation, design of continuous counter current distillation process - McCabe Thiele
method, (binary components only) - Packed column distillation, HTU, NTU and HETP
concepts - Principles of gas absorption, single and multi component absorption, absorption

with chemical reaction, design principles of absorbers, HTU & NTU concepts, industrial
absorbers
LIQUID € LIQUID & SOLID - LIQUID OPERATIONS

Liquid - liquid equilibria, staged and continuous extraction - Solid-liquid Equilibria -
Leaching principles - Adsorption equilibria - Batch and fixed bed adsorption - Ion exchange
drying ‚ Mechanism, drying curves, time of drying, batch and continuous dryers
MEMBRANE SEPARATION PROCESSES

Membrane separation processes -Types liquid and gas - Membrane processes - Complete
mixing - Cross flow and counter current flow models - Concentration polarization and
membrane fouling, ultra filtration and micro filtration
TEXT BOOKS
1. Treybal, R.E., Mass Transfer Operations, 3rd Edition, McGraw Hill, New Delhi, 3rd
Edition, 1981
2. Geankoplis, C.J., Transport Processes and Unit Operations, Prentice Hall of India,
New Delhi, 3rd Edition, 2002.
REFERENCES
1. Coulson, J.M., Richardson, J.F., Backhurst, J.R. and Harker, J.M., Coulson and
Richardson•s Chemical Engineering, Volume II, Butter worth Heinemann, Oxford, 5th
Edition, 2002.
L T P C
CHE 355 BIOPROCESS PLANT DESIGN AND ECONOMICS
3 0 0 3
Course Outcomes:
CO1: Describe about Business organizations processes
CO2: Describe Project design and development
CO3: Summarize Economics of plant Design
CO4: Discuss Process optimization for economic production
CO5: Choose Quality control requirements for production

CO1 H M
CO2 M H
CO3 H M
CO4 M H M
CO5 M H
PROCESS ECONOMICS AND BUSINESS ORGANIZATIONS

Definition of bioprocess - Bioprocess economics - Importance of various M-inputs -

Globalization concept - Competition by dumping and it•s effect on plant size - Status of India
with adjoining ASIAN countries (Singapore, Malaysia, Indonesia etc) - Project profile
concept ,details - Structure and types of organizations - Simple management principles
PROJECT DESIGN AND DEVELOPMENT

Choosing a project - Market survey, importance of techno-economic - Viability studies -
Sourcing of processes - Process alternatives - Fixing most economic processes technology ‚
Scanning - Plant location principles - Plant lay out - Process flow sheets, preparation of
budgetary investment and production costs
COST ESTIMATION, PROFITABILITY AND ACCOUNTING

Capital investment - Concept of time -Value of money - Source sink concept of profitability,
capital costs, depreciation, estimation of capital costs, manufacturing costs, working capital,
profitability standards, project profitability evaluation - Alternative investments and
replacements - Annual reports - Balance sheets - Performance analysis
PROCESS OPTIMIZATION TECHNIQUES

Optimum design - Design strategy, economic - Balance, different unit operations with single
and multiple variables
QUALITY AND QUALITY CONTROL

Current good manufacturing practices, concepts of quality control in 20 th century, elements
of quality control envisaged by ISI since 1947 - Emergence of statistical process control
(SPC) - Simple SPC concept details - Fundamental concepts of ISO 9000 Quality System
and the various requirements for ISO certification
TEXT BOOK
1. Peters, and Timmerhaus., Plant design and Economics for Chemical Engineers,
McGraw Hill, New Delhi, 4th Edition, 1989.
REFERENCE
1.
Rudd, D.F., Watson., Strategy of Process Engineering, John Wiley, Singapore, 3rd
Edition, 1987
L T P C
CHE 356 CHEMICAL AND BIO- THERMODYNAMICS
3 0 0 3

CO1: Apply the laws of thermodynamics to chemical and bio processes
CO2: Calculate differences in thermodynamic properties using equations of state, charts and
tables
CO3: Solve problems dealing with multiphase physical and reactive systems
CO4: Explain the molecular basis of thermodynamics
CO5: Interpret thermodynamic data for applications in chemical engineering processes and
biological sciences


CO1 H M M
CO2 H M
CO3 H M M
CO4 H M
CO5 H M M
FIRST LAW OF THERMODYNAMICS

Joules Experiment, Internal Energy, Formulation of first law of Thermodynamics -
Thermodynamic state and state functions, enthalpy, reversible processes ‚ Steady-state-flow
process - Heat effects accompanying phase change, standard heat of reaction, formation and
combustion - Effect of temperature on standard heat of reaction - Heat effects of industrial
reactions
SECOND LAW OF THERMODYNAMICS

Statement of Second law ‚ The heat engine ‚ Thermodynamic temperature scales ‚ Carnot
cycle for an ideal gas - Entropy, Entropy changes of an ideal gas ‚ Principle of the increase
of Entropy; Mathematical statement of the second law ‚ Entropy from the macroscopic view
point
THERMODYNAMIC PROPERTIES OF MIXTURES AND SOLUTION

Partial molar properties and relation with total mixture property - Gibbs'-Duhem equation,
chemical potential, mixing rules, departure functions for real gas mixtures, fugacity and
fugacity coefficients of real gas mixtures. Ideal and non-ideal solutions - Dilute solutions,
thermodynamic properties of solutions, Lewis-Randall rule, Raoult's Law and Henry's Law
PHASE EQUILIBRIA
Phase transitions and phase equilibria - Gibbs' phase rule, binary vapor-liquid equilibria
(VLE),VLE at low and high pressures, modified Raoult's law, VLE for miscible, partially
miscible and immiscible systems, VLE using equations of state, liquid-liquid equilibria
(LLE), adsorption equilibria - Common adsorption isotherms
CHEMICAL REACTION EQUILIBRIA

Extent of reaction, equilibrium constant, effect of operating conditions on equilibrium
conversion, determination of equilibrium compositions for homogeneous gas-phase reactions
- Single and multiple reactions, liquid-phase reaction equilibria - Electrochemical equilibria
and applications
TEXT BOOKS

1. Smith, J.M., Van Ness H.C., Abbot M.M., Chemical Engineering Thermodynamics,
McGraw-Hill, New Delhi, 6th Edition, 2001.
2. Narayanan, K.V., A Text Book of Chemical Engineering Thermodynamics, Prentice
Hall India, New Delhi, 2nd Edition, 2001.
REFERENCE
1. Nag,P.E., Engineering Thermodynamics, McGraw Hill, New Delhi, 2nd Edition,
1995.
L T P C
CHE314 COLLOIDS & SURFACE SCIENCE
3 0 0 3
INTRODUCTION
Hamaker's analysis for interparticle attractive forces, experiments verifying Van der Waals
interactions between surfaces, Lifshitz macroscopic theory for the Hamaker constant,
Parsegian, Ninham's approximation to Lifshitz theory, Casimir and Polder's correction for
relaxation effects - Example calculations of Hamaker constants for several specific metal,
polymer, and ceramic systems - Influence of other types of interparticle forces
HIERARCHY OF SURFACE CHEMICAL MODELS

Hierarchy of surface chemical models for surface charging - Monoprotic surface charging
systems lattices and organic acids, metallic and non,oxide systems - Role of surface oxygen
in dictating surface charge for metal and non-oxide ceramic systems
ELECTRIC DOUBLE LAYER

Isolated electric double layer - Overlap of the double layer for interacting particles - Free
NRGs of isolated and interacting double layers, repulsive NRG due to overlapping double
layers - Derjaguin approximation for the interaction of spherical particles - Concept of the
critical coagulation concentration, - Influence of salt concentration, ionic strength, and ionic
size- Influence of surface charge for monoprotic surface charge systems - Role of surface
charging in the dispersion of solids in non, aqueous systems
STABILIZATION OF PARTICLES WITH NON- IONIC POLYMERS

Criteria for stabilization of particles with non-ionic polymers - Role of polymer solubility in
stabilization - Role of co, and ter, polymers in providing stabilization reconciling surface
attachment with polymer extension from the surface - Impenetrable barrier model for
polymeric stabilization - Compression model by Bagchi for polymeric dispersion -
Interpenetration and compression model for polymeric dispersion - Other assumptions with
respect to the relative contribution of the Hamaker constant toward stabilization with
polymers - Selection criteria for polymeric dispersants for specific types of material systems
- Polymeric dispersion of nanometer size particles
FEATURES OF POLYELECTROLYTE

Features of polyelectrolyte that contribute to their dispersing power - pKa, molecular size and
distribution, type of polymer - Criteria for polyelectrolyte adsorption to charged surfaces -
Role of pKa - Monitoring adsorption via solution depletion, EM scattering, and zeta potential
measurements, polyelectrolyte conformation at charged surfaces - Combined electrostatic
and impenetrable barrier model for dispersion of particles with polyelectrolytes - Some other
concepts regarding nonionic dispersants in aqueous systems, interaction of polyelectrolyte
with ionic species in solution
TEXT BOOKS
1. Hiemenz, P.C, Raj Rajagopalan., Principles of Colloids and Surface Chemistry,
Marcel Dekker, New York, 3rd Edition, 1997.
2. De Keizer., Johannes Lyklema., Hans Lyklema., Fundamentals of Interface and
Colloid Science, Elsevier, New Delhi, 2nd Edition, 2000.
REFERENCE
1. Milling,A.J., Surface Characterization Methods Principles, Techniques and
Applications, (Surfactant Science Series- V, 87), CRC, New York, 1st Edition, 1999.
L T P C
CIV369 ENVIRONMENTAL IMPACT ASSESSMENT
3 0 0 3
INTRODUCTION
Impact of development projects under Civil Engineering on environment - Environmental
Impact Assessment (EIA) - Environmental Impact Statement (EIS) ‚ EIA capability and
limitations ‚ Legal provisions on EIA.
METHODOLOGIES
Methods of EIA ‚ Check lists ‚ Matrices ‚ Networks ‚ Cost-benefit analysis ‚ Analysis of
alternatives ‚ Case studies.
PREDICTION AND ASSESSMENT

Assessment of Impact on land, water and air, noise, social, cultural flora and fauna,
Mathematical models, public participation ‚ Rapid EIA.
ENVIRONMENTAL MANAGEMENT PLAN

Plan for mitigation of adverse impact on environment - options for mitigation of impact on
water, air and land, flora and fauna, addressing the issues related to the Project Affected
People ‚ ISO 14000
CASE STUDIES
EIA for infrastructure projects - Bridges - Stadium - Highways - Dams - Multi-storey
Buildings - Water Supply and Drainage Projects.
TEXT BOOKS
1. Canter,L., Environmental Impact Assessment, McGraw-Hill Inc., New Delhi, 1996.
2. Shukla, S.K. and Srivastava, P.R., Concepts in Environmental Impact Analysis,
Common Wealth Publishers, New Delhi, 1992.

REFERENCES
1. John G. Rau and David C Hooten (Ed)., Environmental Impact Analysis Handbook,
McGraw-Hill Book Company, New York, 1990.
2. Environmental Assessment Source book, Vol. I, II and III. The World Bank,
Washington, D.C., 1991.
3. Judith Petts, Handbook of Environmental Impact Assessment Vol. I and II,
Blackwell Science, 1999.
L T P C
CIV463 SOLID WASTE MANAGEMENT
3 0 0 3
SOURCES AND TYPES OF MUNICIPAL SOLID WASTES

Sources and types of solid wastes - Quantity - Factors affecting generation of solid wastes;
characteristics - methods of sampling and characterization; Effects of improper disposal of
solid wastes - public health effects - Principle of solid waste management ‚ social and
economic aspects; Public awareness; Role of NGOs; Legislation
ON-SITE STORAGE AND PROCESSING

On-site storage methods - Materials used for containers - on-site segregation of solid wastes -
public health and economic aspects of storage - options under Indian conditions - Critical
Evaluation of Options
COLLECTION AND TRANSFER

Methods of Collection - types of vehicles - Manpower requirement - collection routes;
transfer stations - selection of location, operation and maintenance; options under Indian
conditions
OFF-SITE PROCESSING
Processing techniques and Equipment; Resource recovery from solid wastes - composting,
incineration, Pyrolysis - options under Indian conditions
DISPOSAL
Dumping of solid waste; sanitary land fills - site selection, design and operation of sanitary
landfills - Leachate collection and treatment
TEXT BOOKS
1. George Tchobanoglous, Hilary Theisen and Samuel A, Vigil Integrated Solid Waste
Management, McGraw-Hill Publishers, 1993.
2. B.Bilitewski, G.HardHe, K.Marek, A.Weissbach, and H.Boeddicker, ƒWaste
Management„, Springer.
REFERENCES
1. Manual on Municipal Solid Waste Management, CPHEEO, Ministry of Urban
Development, Government of India, New Delhi, 2000

2. R.E.Landreth and P.A.Rebers., Municipal Solid Wastes - problems and Solutions,

Lewis Publishers, 1997.
3. Bhide A.D. and Sundaresan B.B., Solid Waste Management in Developing Countries,
INSDOC, 1993.
L T P C
CIV416 INDUSTRIAL WASTEWATER MANAGEMENT
3 0 0 3
Course Outcomes:
CO1: Describe the nature and composition of industrial pollutants, their origin and their
impact on the environment
CO2: Explain the principles of various processes available for wastewater treatment
CO3: Choose methods for waste minimization and water conservation
CO4: List the problems associated with the operation of industrial wastewater treatment
facilities and provide an explanation of the causes and possible solutions
CO5: Determine the toxicity levels of industrial effluents

CO1 H M M
CO2 H M M
CO3 H M
CO4 H M
CO5 M H
INTRODUCTION
Industrial scenario in India- Industrial activity and Environment - Uses of Water by industry -
Sources and types of industrial wastewater - Industrial wastewater and environmental
impacts - Regulatory requirements for treatment of industrial wastewater - Industrial waste
survey - Industrial wastewater generation rates, characterization and variables - Population
equivalent - Toxicity of industrial effluents and Bioassay tests
INDUSTRIAL POLLUTION PREVENTION

Prevention Vs Control of Industrial Pollution - Benefits and Barriers - Source reduction
techniques - Waste Audit - Evaluation of Pollution prevention options - Environmental
statement as a tool for pollution prevention - Waste minimization Circles
INDUSTRIAL WASTEWATER TREATMENT

Equalisation - Neutralisation - Oil separation - Flotation - Precipitation - Heavy metal
Removal - Refractory organics separation by adsorption - Aerobic and anaerobic biological
treatment - Sequencing batch reactors - High Rate reactors ‚ Chemical oxidation - Ozonation
- Photocatalysis - Wet Air Oxidation - Evaporation - Ion Exchange - Membrane
Technologies - Nutrient removal

WASTEWATER REUSE AND RESIDUAL MANAGEMENT

Individual and Common Effluent Treatment Plants - Joint treatment of industrial wastewater
- Zero effluent discharge systems ‚ Quality requirements for Wastewater reuse - Industrial
reuse - Disposal on water and land - Residuals of industrial wastewater treatment -
Quantification and characteristics of Sludge - Thickening, digestion, conditioning,
dewatering and disposal of sludge - Management of RO rejects
CASE STUDIES
Industrial manufacturing process description, wastewater characteristics, source reduction
options and waste treatment flow sheet for Textiles - Tanneries - Pulp and paper - metal
finishing- Petroleum Refining - Pharmaceuticals - Sugar and Distilleries - Food Processing -
fertilizers - Thermal Power Plants and Industrial Estates
REFERENCES
1. Eckenfelder, W.W., Industrial Water Pollution Control, McGraw-Hill, 1999
2. Arceivala, S.J., Wastewater Treatment for Pollution Control, Tata McGraw-Hill,
1998
3. Frank Woodard, Industrial waste treatment Handbook, Butterworth Heinemann, New
Delhi, 2001
4. World Bank Group Pollution Prevention and Abatement Handbook - Towards
Cleaner Production, World Bank and UNEP, Washington D.C.1998
5. Paul L. Bishop Pollution Prevention: - Fundamentals and Practice, McGraw-Hill
International, 2000
L T P C
CSE103 DATA STRUCTURES
3 0 0 3
INTRODUCTION
The Role of Algorithms in Computing - Algorithms, Algorithms as a technology - Structures
in C ‚ Implementation of structures - Unions in C - Implementation of unions - Structure
parameters - Recursive definition and processes: Factorial function - Fibonacci sequence -
Recursion in C - Efficiency of recursion.
STACKS, QUEUES AND HASHING

Abstract Data Types- Stacks-Stack applications- Balancing symbols, Infix to postfix
expression conversion, Postfix Expression evaluation, Function calls- Queues- Linked lists-
Hash Tables - Direct-address tables, Hash tables, Hash functions - Open addressing.
TREES
Tree Terminologies - Binary tree - Binary tree traversal - Expression tree construction-
Binary Search Trees- Querying a binary search tree, Insertion and deletion‚ AVL trees-
rotations, insertion. B-Trees-Definition of-trees- Basic operations on B-trees- insertion and
deletion.
SORTING AND SEARCHING

Priority Queues (Heaps) ‚ Model ‚ Simple implementations ‚ Binary Heap-Properties.

Sorting-Bubble sort, insertion sort, selection sort, shell sort, Heap sort, quick sort, Radix sort,
Merge sort. Searching - Linear search, Binary search.
GRAPHS
Graph Terminologies - Representations of Graphs, Breadth-first search, Depth-first search,
Topological sort, strongly connected components. Minimum Spanning Trees- Growing a
minimum spanning tree - The algorithms of Kruskal and Prim-Shortest paths in directed
acyclic graphs, Dijkstra's algorithm, All Pairs Shortest Paths - The Floyd - Warshall
algorithm.
TEXT BOOK
1. Weiss M. A., Data Structure and Algorithm Analysis in C, Addison Wesley,.
2. Cormen T. H., Leiserson C. E., Rivest R. L. and Stein C., Introduction to Algorithms,
Prentice Hall India, 2nd Edition, 2002.
REFERENCES
1. Aaron Tenenbaum M, Yeedidyah Langsam, Moshe Augenstein J., (2004) Data
structures using C, Pearson Education.
2. Horowitz E., Shan S, Fundamentals of Data Structures, Pittman, 1977.
L T P C
EIE409 BIOMEDICAL INSTRUMENTATION
3 0 0 3
ANATOMY, PHYSIOLOGY AND TRANSDUCERS

Brief review of human physiology - anatomy ‚ cell structures ‚ electrical activities -
mechanical activities - chemical activities ‚ action potential - resting potential ‚ different
types of electrodes ‚ sensors used in biomedicine ‚ selection criteria for transducers -
electrodes ‚ necessity for low noise pre-amplifiers ‚ difference amplifiers ‚ difference
amplifiers ‚ chopper amplifiers ‚ electrical safety ‚ grounding ‚ isolation
MEASUREMENT OF BIOPOTENTIAL AND PHYSIOLOGICAL PARAMETERS

ECG ‚ Phonocardiography ‚ Neurophysiology ‚ Central nervous system ‚ EEG ‚
Respiratory system ‚ Muscular system - EMG, - Eye ‚ ERG, Physiological Transducers -
Measurement of Blood pressure ‚ Blood flow - Cardiac output measurement ‚ heart rate ‚
respiration rate ‚ measurement of lung volume ‚ Oximeters ‚ Audiometer
THERAPEUTIC AND SURGICAL EQUIPMENTS

Electro Surgical unit ‚ short wave - microwave diathermy ‚ Laser surgical unit ‚ Anesthesia
machine ‚ Pacemakers ‚ Total artificial heart (TAH) ‚ Dialyser ‚ Heart lung machine ‚
Defibrillators ‚ Ventilators ‚ Nerve stimulators ‚ centralized and Bedside patient monitoring
system ‚ Nerve stimulators
BIOMEDICAL EQUIPMENTS AND ELECTRICAL SAFETY

-Flame photometer ‚ spectrophotometer ‚ chromatography ‚ pH, pCO2, analysis ‚ sterilizers
‚ Electrical safety hazards in hospitals

IMAGING SYSTEMS AND TELEMETRY

Computerized Tomography (CT) ‚ MRI instrumentation ‚ Ultrasound scanner ‚ X-ray
machine ‚ Fluroscopic techniques ‚ angiography ‚ Cardiac catherisation lab ‚ Echo
cardiograph ‚ vector cardiograph ‚ Biotelemetry
TEXT BOOKS
1. Kandpur, R.S., Handbook of Biomedical Instrumentation, TMH
2. Richard Aston, Principles of Biomedical Instrumentation and Measurement, Merrill
publishing company, 1990.
REFERENCES
1. Arumugam, M., Biomedical Instrumentation, Anuradha Agencies, Publishers,
Kumbakonam, 1992.
2. Geddes, L.A. and Baker, L.E., Principles of Applied Biomedical Instrumentation,
John wiley and Sons, 1989.
L T P C
EIE416 OPTIMIZATION TECHNIQUES
3 0 0 3
INTRODUCTION TO OPTIMIZATION
Engineering applications - classical optimization techniques ‚ linear programming ‚ simplex
method ‚ duality - karmankar•s method
MULTIVARIABLE OPTIMIZATION
With equalities - without equalities - weighted sum method ‚ E constraint method - goal
programming
NON LINEAR PROGRAMMING

One dimensional minimization - geometric programming ‚ dynamic programming - queuing
theory - game theory
EVOLUTIONARY COMPUTATION TECHNIQUES

Genetic algorithm ‚ EP ‚ PSO ‚ ANT ‚ NSGA-II
APPLICATION
Conventional - Evolutionary optimization methods for control problems
TEXT BOOKS
1. Kalyanmoy deb, Multi objective evolutionary Algorithms, John wiley & sons, 1st
Edition, 2002.
2. Rao, S.S., Optimization: theory and applications, 4th edition, New age international
publishers, 4th Edition, 2002.
REFERENCES

1. Kanti Swarup, et al, Operation Research, Sultan Chand, 11th Edition, 2003.
L T P C
INT 303 DATABASE MANAGEMENT SYSTEMS
3 0 0 3
INTRODUCTION AND CONCEPTUAL MODELING

Introduction to File and Database systems- Database system structure ‚ Data Models ‚
Introduction to Network and Hierarchical Models ‚ ER model ‚ Relational Model ‚
Relational Algebra and Calculus.
RELATIONAL MODEL
SQL ‚ Data definition- Queries in SQL- Updates- Views ‚ Integrity and Security ‚
Relational Database design ‚ Functional dependences and Normalization for Relational
Databases (up to BCNF).
DATA STORAGE AND QUERY PROCESSING

Record storage and Primary file organization- Secondary storage Devices- Operations on
Files- Heap File- Sorted Files- Hashing Techniques ‚ Index Structure for files ‚ Different
types of Indexes- B-Tree - B+Tree ‚ Query Processing.
TRANSACTION MANAGEMENT
Transaction Processing ‚ Introduction- Need for Concurrency control- Desirable properties
of Transaction- Schedule and Recoverability- Serializability and Schedules ‚ Concurrency
Control ‚ Types of Locks- Two Phases locking- Deadlock- Time stamp based concurrency
control ‚ Recovery Techniques ‚ Concepts- Immediate Update- Deferred Update - Shadow
Paging.
CURRENT TRENDS
Object Oriented Databases ‚ Need for Complex Data types ‚ OO data Model- Nested
relations - Complex Types- Inheritance Reference Types - Distributed databases-
Homogenous and Heterogenous- Distributed data Storage ‚ XML ‚ Structure of XMLData-
XML Document- Schema- Querying and Transformation. ‚ Data Mining and Data
Warehousing.
TEXT BOOKS
1. Abraham Silberschatz, Henry F., Korth and Sudarshan, S., Database System
Concepts, McGraw-Hill, 4th Edition, 2002.
REFERENCES
1. Ramez Elmasri and Shamkant B. Navathe, Fundamental Database Systems, Pearson
Education, 3rd Edition, 2003.
2. Raghu Ramakrishnan, Database Management System, Tata McGraw-Hill Publishing
Company, 2003.
3. Hector Garcia‚ Molina., Jeffrey D.Ullman and Jennifer Widom., Database System
Implementation- Pearson Education, 2000.

4. Peter Rob and Corlos Coronel., Database System, Design, Implementation and
Management, Thompson Learning Course Technology, 5th Edition, 2003.
HUMANITIES ELECTIVES
L T P C
HSS001 TOTAL QUALITY MANAGEMENT
3 0 0 3
Course Outcomes:
CO1:Explain the role and nature of quality in evolving international economic conditions
CO2:Describe the quality encounter process, including supporting facilities and customer
requirements/characteristics
CO3:Classify quality measurement methods and continuous improvement process
CO4:Discuss Quality Management strategy methods, including identification, development,
implementation and feedback processes
CO5:Select quality recovery processes and their role in the marketing process

CO1 M M H M H H
CO2 M M M H H H
CO3 M M H H H
CO4 M M M M H
CO5 M M M H
INTRODUCTION TO QUALITY MANAGEMENT

Definitions ‚ TOM framework, benefits, awareness and obstacles. Quality ‚ vision, mission
and policy statements. Customer Focus ‚ customer perception of quality, Translating needs
into requirements, customer retention. Dimensions of product and service quality. Cost of
quality.
PRINCIPLES AND PHILOSOPHIES OF QUALITY MANAGEMENT

Overview of the contributions of Deming, Juran Crosby, Masaaki Imai, Feigenbaum,
Ishikawa, Taguchi, Shingeo and Walter Shewhart. Concepts of Quality circle, Japanese 5S
principles and 8D methodology.
STATISTICAL PROCESS CONTROL AND PROCESS CAPABILITY

Meaning and significance of statistical process control (SPC) ‚ construction of control charts
for variables and attributed. Process capability ‚ meaning, significance and measurement ‚
Six sigma concepts of process capability. Reliability concepts ‚ definitions, reliability in
series and parallel, product life characteristics curve. Business process re-engineering (BPR)
‚ principles, applications, reengineering process, benefits and limitations.
TOOLS AND TECHNIQUES FOR QUALITY MANAGEMENT

Quality functions development (QFD) ‚ Benefits, Voice of customer, information

organization, House of quality (HOQ), building a HOQ, QFD process. Failure mode effect
analysis (FMEA) ‚ requirements of reliability, failure rate, FMEA stages, design, process and
documentation.
TAGUCHI TECHNIQUES
Taguchi techniques ‚ introduction, loss function, parameter and tolerance design, signal to
noise ratio. Seven old (statistical) tools. Seven new management tools. Bench marking and
POKA YOKE.
REFERENCES:
1. Dale H.Besterfield et al, Total Quality Management, Thrid edition, Pearson
Education (First Indian Reprints 2004).
2. Shridhara Bhat K, Total Quality Management ‚ Text and Cases, Himalaya Publishing
House, First Edition 2002.
3. William J.Kolarii, Creating quality, Mcgraw Hill, 1995
4. Poornima M.Charantimath., Total quality management, Pearson Education, First
Indian Reprint 2003.
L T P C
HSS002 ENGINEERING MANAGEMENT
3 0 0 3
Introduction - Demand and Revenue Analysis - Demand Forecasting - Production Analysis -

Cost and Supply Analysis, Price and output Determination - Investment Analysis - Plant
Location - Economic Optimization.
Types of Business Organisation, Forms, Planning - Organizing - Designing effective

organisations - Coordination.
Human Resource Development - Motivating individuals and workgroups - Leadership for

Managerial Effectiveness - Team working and Creativity - Managerial Communication -
Personal Management ‚ Time Management - Stores Management - Career Planning.
Financial Management - Product development - Management techniques in product

development - Nature of controlling - Operations Management - Just-in-Time.
Managing World Economic Change - The global environment - Multinational Strategies -

Economic Cycles and Director Investment - Change and Organisation Development -
Managerial Ethics and Social responsibilities.
REFERENCES:
1. Harold Koontz& Heinz Weihrich - Essentials of Management Tata McGraw Hill
publishing company Ltd.
2. Koontz, Weihrich& Aryasri ‚ Priniciples of Management Tata McGraw Hill
publishing company Ltd.
3. Tripathi& Reddy - Priniciples of Management Tata McGraw Hill publishing
company Ltd.

4. Hampton ‚ Management Tata McGraw Hill publishing company Ltd.

5. L.M.Prasad - Principles of Management
L T P C
HSS004 INDUSTRIAL PSYCHOLOGY
3 0 0 3
The role of the psychologist in industry, the field of occupational Psychology: Study of
behaviour in work situation and applications of Psychological principles to problems of
selection, Placement, Counseling and training
Design of work environments: Human engineering and physical environment techniques of

job analysis, Social environment - Group dynamics in Industry Personal psychology:
Selection, training, placement, promotion, counseling, job motivations, job satisfaction.
Special Study of problem of fatigue, boredom and accidents, Consumer behavior; study of
consumer preference, effects of advertising, Industrial morale The nature and scope of
engineering psychology, its application to industry.
Efficiency at work: the concept of efficiency, the work curve, its characteristics. The work
methods; hours of work, nature of work, fatigue and boredom, rest pauses. The personal
factors; age abilities, interest, job satisfaction the working environment: noise, illumination,
atmospheric conditions. Increasing efficiency at work; improving the work methods; Time
and motion study, its contribution and failure resistance to time and motion studies, need for
allowances in time and motion study.
Work and equipment design: Criteria in evaluation of job-related factor, job design,
human factors, Engineering information, input processes, mediation processes, action
processes, methods design, work space and its arrangement, human factors in job design.
Accident and Safety: The human and economic costs of accidents, accident record and
statistics, the causes of accidents situational and individual factors related to accident
reduction
REFERENCES:
1. Tiffin,J and McCormic E.J.: Industrial Psychology, (Prentice Hall), 6th Edition, 1975
2. McCormic E.J.: Human Factors engineering and design (McGraw Hill), Mair, N.R.F.:
Principles of Human relations 4th Edition, 1976
3. Gilmer: Industrial Psychology
4. Ghiselli & Brown: Personnel and Industrial Psychology
5. Myer: Industrial Psychology
6. Dunnete, M.D.: Handbook of Industrial and Organizational Psychology
7. Blum & Taylor: Industrial Psychology

L T P C
HSS005 CONSUMER PSYCHOLOGY
3 0 0 3
Consumer behaviour ‚ introduction ‚ Consumer behaviour ‚ concepts ‚ dimensions of

consumer behaviours ‚ application of consumer behaviour knowledge in marketing decisions
‚ approaches to the study of consumer behaviour.
Motivation, ability and opportunity; exposure, attention and perception Categorizing and
comprehending information Attitude formation and change ‚ memory and retrieval Process
of decision making ‚ psychographics Consumer behaviour outcomes ‚ consumer welfare
Group dynamics and consumer reference groups ‚ Family ‚ Social class cultural and sub-
cultural aspects ‚ cross cultural consumer behaviour.
Personal influence and opinion leadership ‚ diffusion of innovations ‚ consumer decision ‚

making process ‚ models of consumer decision process ‚ Nicosia- Howard Sheth and Engel-
Kollat model- post purchase behaviour
Consumerism ‚ consumer protection ‚ difficulties and challenges in predicting consumer

behaviour ‚ online consumer behaviour ‚ organizational and industrial buyer behaviour ‚
consumer behaviour in Indian context ‚ emerging issues.
REFERENCES:
1. David L.Loudon, Albert J Della Bitta, ƒConsumer Behaviour„, McGraw Hill, New
Delhi 2002.
2. Jay D. Lindquist and M.Joseph sirgy, ƒShopper, buyer & consumer Behaviour,
Theory and Marketing application„, Biztantra Publication, New Delhi 2005.
3. Sheth Mittal, ƒConsumer Behaviour A Managerial Perspective„, Thomson Asia (P)
Ltd., Singapore, 2003.
4. K.K.Srivastava, ƒConsumer Behaviour in Indian Context„, Goal Gotia Publishing Co,
New Delhi 2002.
5. S.L. Gupta & Sumitra Pal, ƒConsumer Behaviour an Indian Perspective„, Sultan
Chand, New Delhi 2001.
6. Ms.Raju, Dominique Xavedel, ƒConsumer behaviour, Concepts Applications and
Cases„, Vikas publishing house (P) Ltd., New Delhi ‚ 2004.

L T P C
HSS006 PROFESSIONAL ETHICS
3 0 0 3
Course Outcomes:
CO1: Understand the importance of balancing professional and personal commitments
CO2: Recognize the importance of avoiding conflicts of interest at the workplace
CO3: Explain how ethical theories help in resolving moral dilemmas confronting
professionals
CO4: Know the dividing line between loyalty to employers and commitments to public
welfare
CO5: Appreciate the need to avoid gender bias and related discrimination at the workplace

CO1 H M M M M H
CO2 M H M H M M H
CO3 M M H H
CO4 M M H H
CO5 M H H H
Functions of Being a Manager ‚ Stock holder and stakeholder management. Ethical

treatment of employees - ethical treatment of customers- supply chain management and other
issues
Senses of Ethics ‚ Variety of moral issues ‚ Types of inquiry ‚ Moral dilemmas. Moral
Autonomy ‚ Kohlberg•s theory ‚ Gilligan•s theory ‚ Consensus and Controversy ‚
Professions and Professionalism ‚ Professional ideals and virtues ‚ Theories about right
action ‚ Self-interest ‚ Customs and religion ‚ Use of Ethical Theories
Corporate social responsibility. Collegiality and loyalty ‚ Respect for Authority ‚ Collective
Bargaining ‚ Confidentiality ‚ Conflicts of Interest ‚ Occupational Crime ‚ Professional
Rights ‚ Employee Rights ‚ Discrimination
Moral imagination, stake holder theory and systems thinking. One approach to management
Decision ‚ making Leadership
Multinational Corporations ‚ Environmental Ethics ‚ Computer Ethics ‚ Weapons

Development ‚ Engineers as Managers ‚ Consulting Engineers ‚ Engineers as Expert
Witnesses and Advisors ‚ Moral Leadership ‚ Sample code of conduct.

REFERENCES:
1. Mike Martin and Roland Schinzinger, Ethics in Engineering, McGraw Hill, New
York, 1996
2. Charles D Fledderman, Engineering Ethics, Prentice Hall, New Mexico, 1999.
3. Laura Schlesinger, How Could You Do That: The Abdication of Character, Courage,
and Conscience, Harper Collins, New York, 1996.
4. Stephen Carter, Integrity, Basic Books, New York 1996.
5. Tom Rusk, The Power of Ethical Persuasion: From Conflict to Partnership at Work
and in Private Life, Viking, New York, 1993
L T P C
HSS007 OPERATIONS MANAGEMENT
3 0 0 3
INTRODUCTION TO PRODUCTION AND OPERATION MANAGEMEN

Production and Operations Management (POM) ‚ Need, History, System, Types, functions
and communication in POM.
MATERIAL AND INVENTORY MANAGEMENT:

Material Management (MM) ‚ Handling Technology (Robots, Automated storage and
retrieval systems (ASRS) and methods (JIT, / Kanban, ABC Systems).
Independent Demand Inventory Models ‚ Fixed order system, Basic EOQ, EBQ Models,
Quantity discount models.
Dependent Demand Inventory models ‚ MRP and MRP II systems Introduction to ERP, e-
business and e-operations strategies.
PLANNING AND FORECASTING:

Introduction to Strategic, Tactical, Operational, Aggregate and Capacity Planning. Planning
Product design and development ‚ Applications of CAD, CAM, Computer Integrated
Manufacturing
FORECASTING AND SCHEDULING:

Forecasting ‚ Types, Methods (Qualitative and Quantitative), Types of variation in data,
Minimizing forecasting errors and selection of forecasting methods. Johnson•s Algorithm for
job sequencing (n job thro• 2 machines, n jobs thro• 3 machines, n jobs thro• m machines and
2 jobs thro• m machines) Use of Gantt charts, Queuing analysis and Critical Ratios as
methods for job scheduling.
FACILITY, LAYOUT LOCATION AND WORK MEASUREMENT

Facility Location Decisions (FLcD) ‚ . Facility Layout Decision (FlyD) ‚ Types (Fixed
Position, and Production, Process, Flexible), Methodologies (Distance Minimising,
Computer software systems (CRAFT, CORELAP, ALDEP), Line Balancing and
performance ratios, work measurement methods (WM) - Time study, methods-time
measurement,

REFERENCES:
1. R.Paneer Selvam, Production and Operations Management, Prentice Hall of India,
2002.
2. Sang M Lee and Marc J Schniederjans, Operation Management, All India Publishers
and Distributors, First Indian edition 1997.
3. Robert H. Lowson, Strategic operations Management (The new competitive
advantage), Vikas Publishing House, First Indian reprint 2003.
L T P C
HSS008 INTRODUCTION TO ECONOMICS
3 0 0 3
DEFINITION AND SCOPE OF ECONOMICS: Definitions by A. Smith, A. Marshal and

L. Robbins, P.Samuelson and their critical examination. Nature and scope of Economics.
Micro-economics in relation to other branches of Economics.
Law of Demand, Elasticity of demand - price, income and cross, concepts and measurement.
Marshallian theory of consumers• behaviour and its critical examination. Indifference curve
analysis. Price, income and substitution effects. Giffen goods. Engel curve.
MARKET STRUCTURE: Definition of market. Concepts of product and factor markets.

Different types of market: perfect competition, monopoly, imperfect competition,
monopolistic,competition and oligopoly. Demand and Supply schedules. Price determination
under perfect competition in long and short run. Price determination under monopoly.
Discriminating monopoly.
MACRO-ECONOMICS: Meaning, Macro-economic Policy and Its Objectives and

Instruments. National Income and Social Accounting: Concepts, components, and
measurement. Basic circular flow of income model, Unemployment, trade cycle, Inflation:
causes, types, effects and control.
Commercial and Central Banks, Credit creation, monetary policy and tools. Balance of
payments: Items in the balance of payments account, equilibrium in the balance of payments.
REFERENCES:
1. Ackley, G., Macroeconomics: Theory and Policy, Macmillan Publishing Company,
New York, 1978.
2. Gupta, S.B., Monetary Economics, S. Chand & Co., New Delhi, 1994.
3. Ruddar Datt and K.P.M.Sundharam, Indian Economy, S.Chand & Company Ltd.,
New Delhi, 2003.
4. Kindleberger, C.P., International Economics, R.D. Irwin, Home Wood, 1973
5. Lewis, M.K. and P.D. Mizan., Monetary Economics, Oxford University Press, New
Delhi, 2000.
6. Ahuja H.L., Economic Environment of Business, Macroeconomic analysis, S.Chand
& Company Ltd., New Delhi, 2005.
7. Gupta, G.S. Macroeconomics, Theory and Applications, Tata McGraw-Hill
publishing company Ltd., New Delhi, 2001.

8. D.N.Dewedi, Macro economic ‚ Theory and policy, Tata McGraw-Hill publishing

company Ltd., New Delhi, 2001.
9. K.P.M.Sundaram, Money Banking and international Trade, Himalaya Publishing
House.
L T P C
HSS010 INTERNATIONAL TRADE AND FINANCE
3 0 0 3
INTERNATIONAL TRADE
International Trade ‚ Meaning and Benefits ‚ Basis of International Trade ‚ Foreign Trade
and Economic Growth ‚ Balance of Trade ‚ Balance of Payment ‚ Current Trends in India ‚
Barriers to International Trade ‚ WTO ‚ Indian EXIM Policy.
EXPORT AND IMPORT FINANCE

Special need for Finance in International Trade ‚ INCO Terms (FOB, CIF, etc.,) ‚ Payment
Terms ‚ Letters of Credit ‚ Pre Shipment and Post Shipment Finance ‚ Forfaiting ‚ Deferred
Payment Terms ‚ EXIM Bank ‚ ECGC and its schemes ‚ Import Licensing ‚ Financing
methods for import of Capital goods.
FOREX MANAGEMENT
Foreign Exchange Markets ‚ Spot Prices and Forward Prices ‚ Factors influencing Exchange
rates ‚ The effects of Exchange rates in Foreign Trade ‚ Tools for hedging against Exchange
rate variations ‚ Forward, Futures and Currency options ‚ FEMA ‚ Determination of Foreign
Exchange rate and Forecasting.
DOCUMENTATION IN INTERNATIONAL TRADE

Export Trade Documents: Financial Documents ‚ Bill of Exchange- Type- Commercial
Documents - Performa, Commercial, Consular, Customs, Legalized Invoice, Certification of
Origin Certificate Value, Packing List, Weight Certificate, Certificate of Analysis and
Quality, Certificate of Inspection, Health certificate. Transport Documents - Bill of Landing,
Airway Bill, Postal Receipt, Multimodal Transport Document. Risk Covering Document:
Insurance Policy, Insurance Cover Note. Official Document: Export Declaration Forms, GR
Form, PP From, COD Form, Softer Forms, Export Certification, Certification of Origin,
GSPS ‚ UPCDC Norms
EXPORT PROMOTION SCHEMES

Government Organizations Promoting Exports ‚ Export Incentives : Duty Exemption ‚ IT
Concession ‚ Marketing Assistance ‚ EPCG, DEPB ‚ Advance License ‚ Other efforts I
Export Promotion ‚ EPZ ‚ EQU ‚ SEZ and Export House.
REFERENCES
1. Apte P.G., †International Financial Management•‚ Tata McGraw Hill
2. Larceny & Bhattacharya, †International Marketing•- Sultan Chand & Sons.
3. B.M.Wali and AB Kalkumdrikas, †Export Management• - Sterling Publishers Pvt.,
Ltd.

4. Websites of WTO, World Bank, IMF, Ministry of Commerce, ECGC and EXIM
Bank.
INFORMATION SYSTEMS FOR MANAGERIAL L T P C

HSS011
DECISION MAKING 3 0 0 3
INTRODUCTION
Information system ‚ establishing the framework ‚ business model ‚ information system
architecture ‚ evolution of information systems.
INFORMATION SYSTEM
Functional areas, Finance, marketing, production, personnel ‚ levels, Concepts of DSS, EIS,
ES ‚ comparison, concepts and knowledge representation ‚ managing international
information system.
SYSTEM DEVELOPMENT
Modern information system ‚ system development life cycle ‚ structured methodologies ‚
designing computer based method, procedures control, designing structured programs.
IMPLEMENTATION AND CONTROL

Testing security ‚ coding techniques ‚ detection of error ‚ validation ‚ cost benefits analysis
‚ assessing the value and risk information systems.
SOFTWARE ENGINEERING
Software engineering qualities ‚ design, production, service, software specification, software
metrics, and software quality assurance ‚ software life cycle models ‚ verification and
validation.
REFERENCES:
1. Kenneth C. Laudon and Jane Price Laudon, Management Information systems
Managing the digital firm, Pearson Education Asia.
2. Gordon B.Davis, Management Information system: Conceptual Foundations,
Structure and Development, McGraw Hill, 1974.
3. Joyce J. Elam, Case series for Management Information System Silmon and Schuster,
Custom Publishing 1996.
4. Steven Alter, Information system ‚ A Management Perspective ‚ Addison ‚ Wesley,
1999.
5. James AN O• Brein, Management Information Systems, Tata McGraw Hill, New
Delhi, 1999.
6. Turban Mc Lean, Wetherbe, Information Technology Management making
connection for strategic advantage ‚ John Wiley, 1999.
7. Ralph M.Stair and George W.Reynolds Principles of Information Systems ‚ A
Managerial Approach Learning, 2001.

L T P C
HSS013 COST ANALYSIS AND CONTROL
3 0 0 3
INTRODUCTION TO COSTING
Costing, Elements of costing, Types of cost, Preparation of cost sheet
COST ANALYSIS
Marginal costing, Cost - volume ‚ Profit analysis, Break-Even-Analysis, Break - Even-Chart,
Applications.
CONTROL TECHNIQUES
Budgeting and Budgetary control, Types of Budgets , Preparation of purchase Budget,
Flexible budgets ,Cash Budget, Sales Budget, Materials Budget, Master Budget, Zero based
Budgeting.
STANDARD COSTING
Types of Standards, Setting up of standards, Advantages and Criticism of Standard Costing
‚ Control through variances.
ACTIVITY BASED COSTING

Transfer Pricing, Target costing, Life Style Costing, Activity Based Costing (only theory).
REFERENCES:
1. Advanced Cost Accounting and Cost Systems by V.K.Saxena & C.D. Vashist ; Sultan
Chand & Sons Publishers.
2. Advances Cost Accounting by S.P. Jain & K. L. Narang; Kalyani Publishers
3. Cost Management by The Institute of Charted Accountants of India.
L T P C
HSS014 INTRODUCTION TO MARKETING MANAGEMENT
3 0 0 3
MARKETING: Meaning - concept - functions - marketing Planning & implementation

marketing Programmes - Marketing environment ‚ Market Segmentation and consumer
behaviour ‚ Influencing factors, Decision process ‚ Marketing mix ‚ Marketing department.
PRODUCT: Meaning - Product planning - policies - positioning - New product development

Product life cycle ‚ BCG Matrix-branding. Packing, labeling.
PRICING: Pricing objectives ‚ Setting and modifying the price ‚ Different pricing method
Product line pricing and new product pricing
DISTRIBUTION: Nature of Marketing channels - Types of Channel flows - Channel

functions - Channel co-operation, conflict and competition - Direct Marketing
Telemarketing, Internet shopping.

PROMOTION:
Promotion Mix - Advertisement - Message - copy writing ‚ Advertisement budgeting -
Measuring advertisement effectiveness - Media strategy - sales promotion - Personal selling,
publicity and direct marketing
REFERENCES:
1. Philip Kotler., Marketing Management- Analysis Planning and Control, Prentice Hall
of India, New Delhi
2. Cundiff, Still & Govoni., Fundamentals of Modern Marketing, Prentice Hall of India,
New Delhi
3. Ramaswamy. V S & Namakumari. S., Marketing Management-Planning
Implementation and Control, Macmillan Business Books, 2002
4. Jobber., Principles and Practice of Marketing, Mcgraw-Hill.
L T P C
HSS016 ORGANIZATIONAL PSYCHOLOGY
3 0 0 3
FOCUS AND PURPOSE

Definition, need and importance of organizational Behaviour ‚ nature and scope ‚ frame
work.
INDIVIDUAL BEHAVIOUR
Personality ‚ types ‚ factors influencing personality ‚ theories ‚ learning ‚ types of learners
‚ learning theories ‚ organizational Behaviour modification. Attitudes ‚ characteristics ‚
components ‚ formation ‚ measurement. Perceptions ‚ importance ‚ factors influencing
perception ‚ interpersonal perception.
GROUP BEHAVIOUR
Organization structure ‚ formation ‚ groups in organizations ‚ influence ‚ group dynamics ‚
emergence of informal leaders and working norms ‚ group decision making techniques ‚
interpersonal relations ‚ communication ‚ control.
POWER
Leadership styles ‚ theories ‚ leaders Vs managers ‚ sources of power ‚ power centers ‚
power and politics.
DYNAMICS OF ORGANIZATIONAL BEHAVIOURS

Organizational climate ‚ factors affecting organizational climate ‚ importance. Job
satisfaction ‚ determinants ‚ measurements ‚ influence on behavior. Organizational change ‚
importance ‚ stability Vs change ‚ proactive Vs reaction change ‚ the change process ‚
resistance to change ‚ managing change. Organizational development ‚ characteristics ‚
objectives ‚ team building. Organizational effectiveness ‚ perspective ‚ effectiveness Vs
efficiency ‚ approaches ‚ the time dimension ‚ achieving organizational effectiveness.

REFERENCES:
1. Stephen P.Robins, Organisational Behavior, Prentice Hall of India, 9th edition, 2001.
2. Hellriegel, Slocum and Woodman, Organisational Behavior, South-Western,
Thomson Learning, 9th edition, 2001.
3. Schermerhorn, hunt and Osborn, Organisational behavior, John Wiley, 7th edition,
2001.
4. Jit S.Chand, Organisational Behavior, Vikas publishing House Pvt. Ltd. 2nd edition,
2001.
5. Fred Luthans, Organisational Behavior, McGraw Hill Book Co., 1998.
6. New Strom & Davis, Organisational behaviour, McGraw Hill, 2001.
7. Jaffa Harris and Sandra Hartman, Organisational Behaviour, Jaico, 2002.
L T P C
HSS017 INTERNATIONAL ECONOMICS
3 0 0 3
Introduction: The Traditional Theory of International Trade, The Basic Trade Model,
Heckscher-Ohlin-Samuelson Model, Effects of Tariffs & Quotas, Theory of Factor
Movements. New Theories of International Trade and Industrial Policies
The Balance of Payments and National Accounts, Determinants of Exchange Rates The
Exchange-Rate Regime Choice and a Common Currency Area, International Debt and
Currency Crises.
Political Economy of Trade Disputes, the FTA and the WTO. The role of the IMF and
other International Financial Organizations.
Reasons for Protection World Trade, International Movements of Capital. The Balance of
Trade and Other Measures of International Transactions. Export and import policies.
International Macroeconomics European Monetary Unification and the Euro Preferential

Trading Arrangements and the NAFTA International Policies for Economic
Development, Trade Outsourcing and Offshoring
REFERENCES
1. N. Bhagwati, A. Panagariya and T. N. Srinivasan, Lectures on International Trade,
MIT Press, 2nd edition, 1998.
2. M. Obstfeld and K. Rogoff, Foundation of International Macroeconomics, McGraw-
Hill, 1996.
3. Romer, D., Advanced Macroeconomics, McGraw Hill, 1996.

L T P C
HSS018 COMMUNICATION SKILLS
3 0 0 3
COMMUNICATION IN BUSINESS
Systems approach, forms of business communication, management and communication,
factors facilitating communication.
COMMUNICATION PROCESS
Interpersonal perception, selective attention, feedback, variables, listening barriers to
listening, persuasion, attending and conducting interviews, participating in discussions,
debates and conferences, presentation skills, paralinguistic features, oral fluency
development.
BUSINESS CORRESPONDENCE
Business letter. Memos, minutes, agendas, enquiries, orders, sales letters notice, tenders,
letters of application, letter of complaints.
TECHNICAL REPORTS
Format, Choice of vocabulary, coherence and cohesion, paragraph writing, organization.
PROJECT REPORTS
Project proposal, project reports, and appraisal reports.
REFERENCES:
1. Sharan J.Genrson and Steven M.Gerson., Technical Writing ‚ Process and Product,
Pearson Education , 2000.
2. Raymond V.Lesikar, John D. Pettit and Mary E.Flatley ‚ Lesikass Basic
Communication Tata McGraw Will 8th Edition ‚ 1999.
3. Stevel. E. Pauley, Daniel G.Riordan ‚ Technical Report Writing Today ‚ AITBS
Publishing & Distributors, India 5th edition ‚ 2000.
4. Robert L.Shurter, Effective letters in business Third Ed. 1983.
5. McGraith ‚ Basic Managerial Skills for all Prentice Hall of India ‚ 6th Edition 2002.
6. Halliday, M.A.Ky R.Hasan, Cohesion in English, Longman, London 1976.

L T P C
HSS022 BANKING THEORY AND PRACTICE
3 0 0 3
EVOLUTION OF BANKING SYSTEM

Central Banking functions, Reserve Bank control over banks
BANKER - CUSTOMER RELATIONSHIP

Bank as borrowers, customer accounts, duties of paying and collecting bankers
LENDING BY BANKS
RBI control over loans and advances, Securities for loans
AGENCY SERVICES BY BANKS

Banker as bailee, safe deposit vaults, credit cards
CONSUMERS OF BANKING SERVICES

Protection against deficiency in banking services.
REFERENCES:
1. M.L.Tannan, "Tannan's Banking Law and Practice in India", India Law House, New
Delhi, 1997.
2. S.N.Gupta, "The Banking Law in theory and Practice", Vol. I & II, Universal Law
Publishing Co., 1999.
3. M.S.Parthasarathy, "Banking Law-Leading Indian Cases", N.M.Tripathi, 1985.
4. L.C.Goyle, "Law of Banking and Bankers", Eastern Law House, 1995.
L T P C
HSS023 ENTREPRENEURSHIP DEVELOPMENT
3 0 0 3
ENTREPRENEURIAL COMPETENCE
Entrepreneurship concept ‚ Entrepreneurship as a Career ‚ Entrepreneur ‚ Personality
Characteristics of Successful. Entrepreneur ‚ Knowledge and Skills Required for an
Entrepreneur.
ENTREPRENEURIAL ENVIRONMENT
Business Environment - Role of Family and Society - Entrepreneurship Development
Training and Other Support Organisational Services - Central and State Government
Industrial Policies and Regulations - International Business.
BUSINESS PLAN PREPARATION

Sources of Product for Business - Prefeasibility Study - Criteria for Selection of Product -
Ownership - Capital - Budgeting Project Profile Preparation - Matching Entrepreneur with
the Project - Feasibility Report Preparation and Evaluation Criteria.

LAUNCHING OF SMALL BUSINESS

Finance and Human Resource Mobilization Operations Planning - Market and Channel
Selection - Growth Strategies - Product Launching.
MANAGEMENT OF SMALL BUSINESS

Monitoring and Evaluation of Business - Preventing Sickness and Rehabilitation of
Business Units. Effective Management of small Business.
REFERENCES:
1. Hisrich, †Entrepreneurship•, Tata McGraw Hill, New Delhi, 2001.
2. P. Saravanavel, †Entrepreneurial Development•, Ess Pee kay Publishing House,
Chennai -1997.
3. S.S.Khanka, †Entrepreneurial Development•, S.Chand and Company Limited, New
Delhi, 2001.
4. Prasama Chandra, Projects ‚ †Planning, Analysis, Selection, Implementation and
Reviews•, Tata McGraw-Hill Publishing Company Limited, 1996.
5. P.C.Jain (ed.), †Handbook for New Entrepreneurs•, EDII, Oxford University Press,
New Delhi, 1999.
6. Staff College for Technical Education, Manila and Centre for Research and Industrial
Staff Performance, Bhopal, †Entrepreneurship Development•, Tata McGraw-Hill
Publishing Company Ltd., New Delhi, 1998.
L T P C
HSS025 SCIENCE FICTION: AN APPRECIATION
3 0 0 3
This advanced optional course aims at a close analytical study of the impact of science and
technology on Man and his several institutions in society as expressed in science fiction. It
introduces the students to some samples of science fiction, and through a critical discussion
and analysis helps them appreciate the creative link between scientific discoveries and
technological inventions and human civilization. Finally, it will further improve the
student•s ability to analyse scientific concepts in extended discussions and compositions.
Special emphasis will be laid on increasing ability and style.

L T P C
HSS026 GERMAN € I
3 0 0 3
German for science and technology, based on the book †German for science and Technology•
by stecker/Davids, for beginners grammar, noun group; verb, prepositions, pronouns, modal
verbs, compound verbs, reading and translating practice. Simple colloquial German.
L T P C
HSS028 FRENCH € I
3 0 0 3
Definite and indefinite articles ‚ Adjectives ‚ agreement with their nouns ‚ Conjucation of
verbs; to have, to be affirmative, negative and interrogative forms ‚ Possessive adjectives.
Contraction of †of the• †to the• (Singular and Plural)‚ demonstrative adjectives ‚ The three
groups of verbs ‚ present perfect tense with †to have• and †to be•‚ The partitive article ‚
Future tense ‚ immediate future recent past ‚ Reflexive verbs ‚ Present perfect of reflexive
verbs.
SCIENCE, TECHNOLOGY & MEDICINE IN INDIA: L T P C

HSS030
A HISTORICAL PERSPECTIVE 3 0 0 3
This course is designed to provide a broad historical overview of the growth of science,
technology and medicine in India. It aims to explicate to the students the rich scientific and
technological heritage of India and the positive response to the abvent of modern science
through the colonial agency, resulting in the emergence of a viable modern scientific
community in India.
COURSE CONTENTS:
1. Early Indian approaches to the universe, understandings of the physical world,
theories of matter and the quest to transcend existential limitations.
2. Development of science and medicine till the colonial era. Astronomy of the Vedas
and Aryabahata, Varmaihira and Bhaskar I, Brahmagupta, the Arab connection,
Sawai Jai Singh, Mathematics & Geometry: Shulabha Sutra, the Indian numerals, the
decimal notations, calendars and algebra. Medicine and Surgery: Charaka ans
Sushruta Samhitas, Physiology, Anatomy, Meteria Medica, Unani & Ayurveda.
Physical and Botanical Sciences, Antiquity of Indian Chemistry and Alchemy.
3. Advancements in Technology till pre-colonial times: Metallurgy, Artillary, Gun
Powder technology, Persian Wheel, textile technology, the charka, bleaching, dyeing,
Architecture: Monuments, Bridges, and Naval Architecture, Shipbuilding and
Agricultural technologies.
4. Advent of colonial science: Early colonial settlement and scientific exploarations.
The East India company ‚ Surveyors, Botanists and Doctors under the company•s
Service, The Indian Medical Service, Encounters with Indian medicine, Introduction
of stream technologies, Railways, Textiles, Mining, Telegraphs, Canals and Dams.
5. Indian response to modern science, the colonial restrictions and limitation, Science
and nationalism and the emergence of the Indian scientific community.

SUGGESTED READING:
BOOKS:
1. Arnold, David, Science, Technology and Medicine in colonial India Cambridge,
1999.
2. Bose, D.M.S.N. Sen, and B.V. Subarayappa, A Coincise History of science in India,
New Delhi. 1971.
3. Chattopadhayaya, Debiprasad, Science and Society in Ancient India, Calcutta, 1979.
4. Headrick, D.R., The Tools of Empire: Technology and European Imperialism in the
Nineteenth Century, New York, 1981.
5. Jaggi, O.P History of Science, Technology and Medicine in India, 15 volumes, Delhi,
1969-84.
6. Kumar, Deepak, Science and the Raj, Delhi, 1995.
7. Lourdusamy, J.B., (forthcoming) Science and National Consciousness in Bengal, c
1870-1930, Hyderabad, (Jan 2004).
8. Macleod, Roy and Deepak Kumar, eds., Technology and Culture, Delhi, 1982.
9. Qaisar, AJ The Indian Response to European Technology and culture, Delhi, 1982.
10. Sen, S.N. Cultural Heritage of India, Vol vi. Calcutta, 1972.
JOURNALS:
1. Studies in History of Medicine and Science.
2. Bulletin of Indian Institute of History of Medicine.
3. Indian journal of History of Science.
4. Science and culture.
5. Journal of the Asiatic Society of Bengal.

L T P C
HSS033 MODERN SCIENCE IN INDIA
3 0 0 3
The course is deal with the advent and growth of modern science in India under the aegis of
colonialism and the Indian response to it. It would delineate the many ways in which the
entire process was implicated not only in the political axis of colonialism and nationalism but
also in the various socio-cultural developments of the period.
COURSE STRUCTURE:
a) What is modern Science? ‚ The Scientific Revolution and the rise of modern
science in the [1500 ‚ 1700].
b) The advent of modern science in India: Early travelers, missionaries, colonial
settlements and scientific explorations, The east India Company ‚ surveyors,
botanists and doctors under the company•s service, The Asiatic Society
[1500-1757].
c) Institutionalized Colonial Science: The various scientific departments, the
Indian medical service, Introduction of new technologies ‚ Railways, Textiles,
Mining, Telegraphs, Canals and Dams. [1757-1900].
d) Science education in colonial India: Origins of English and western education,
founding of the Universities, the content of science in the curricula, setting up
of technical institutes, the restrictions and limitations of colonialism. [1980-
1930].
e) The Indian response: Response to English Education, its implications for
indigenous society and culture, and the role of social and religious reformers,
the taste for modern science, setting up of indigenous society for the learning
and cultivation of modern science. [1830-1930].
f) The first modern scientists of India: Ramanujam, JC Bose, PC Ray, Meghnad
Saha, C V Raman, S N Bose.
TEXT BOOKS
1. Arnold David Science, Technology and Medicine in Colonial India, Cambridge:
Cambridge University Press 2000.
2. Headrick, D.R., The Tools of Empire: Technology and European Imperialism in the
Nineteenth Century, New York, 1981.
3. Kuma Deepak Eds. Science and Empire: Essays in the Indian context, Delhi:
Anamika Prakashan, 1991, Science and the Raj, 1857-1905. Delhi: Oxford
University Press 1995.
4. Qaisar, A J., The Indian response to European technology and culture, Delhi: Oxford
University Press 1982.
5. Sangwan S Science, Technology and colonization: Indian Experience, Delhi:
Anamika Prakashan, 1990.

L T P C
HSS035 HISTORY OF SCIENCE & TECHNOLOGY
3 0 0 3
Science and Technology in the Primitive society ‚ Development of Science and Technology
in early civilized societies ‚ Science in classical Greece ‚ The rise and Development of early
Indian Science and Technology ‚ European Science and the Renaissance movement ‚
Science and the Revolutionary era (Industrial, American and French Revolution) Growth of
Specific Sciences in Eighteenth and 19th centuries ‚ Heat and Energy Chemistry and Biology
‚ Twentieth Century Science ‚ Physical Sciences ‚ Organization of Indian Science ‚ Recent
advances in Indian Science.

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DEPARTMENT OF BIOTECHNOLOGY

Institute Vision Institute Mission

B. Tech. BIOTECHNOLOGY CURRICULUM

Total Credits (from 1st semester to 8th semester = 177)

LIST OF MAJOR ELECTIVES FOR III YEAR

Code No. Subject L T P C

BIT307 Environmental Biotechnology 3 0 0 3

BIT308 Spectroscopic Methods For Structure Determination 3 0 0 3

BIT309 Food Processing and Technology 3 0 0 3

BIT310 Pharmaceutical Biotechnology 3 0 0 3

BIT311 Healthcare Biotechnology 3 0 0 3

BIT313 Metabolic Engineering 3 0 0 3

BIT314 Drug Design and Development 3 0 0 3

BIT323 Exploring the microbial world 3 0 0 3

CHE352 Bioprocess Instrumentation and Control 3 0 0 3

CHE358 Transport Phenomena in Biological Systems 3 0 0 3

LIST OF MAJOR ELECTIVES FOR IV YEAR

Code No. Subject L T P C

LIST OF MINOR ELECTIVES

Code No. Subject L T P C

LIST OF HUMANITIES ELECTIVES

Code No. Subject L T P C

Course Outcomes Vs Program Outcomes Mapping:

Course Outcomes Vs Program Outcomes Mapping:

EIGEN VALUE PROBLEMS

THREE DIMENSIONAL ANALYTICAL GEOMETRY

ORDINARY DIFFERENTIAL EQUATIONS

Course Outcomes Vs Program Outcomes Mapping:

ACOUSTICS AND STRUCTURE OF SOLIDS

LASER AND FIBRE OPTICS

Course Outcomes Vs Program Outcomes Mapping:

MACROSCOPIC PROPERTIES OF SYSTEMS IN EQUILIBRIUM

electrochemical corrosion - Corrosion control (Sacrificial anode and impressed current

DYNAMICS OF CHEMICAL PROCESSES

INSTRUMENTAL METHODS OF ANALYSIS

Course Outcomes Vs Program Outcomes Mapping:

BASIC ELEMENTS OF C & CONTROL STATMENTS

USER DEFINED FUNCTION FUNCTIONS & STORAGE CLASSES

ARRAYS AND POINTERS

of arrays. Pointer Fundaments - Pointer Declarations - Passing Pointers to Functions -

DYNAMIC MEMORY MANAGEMENT, STRUCTURES & UNIONS

DATA FILES AND UNIX OS

BASIC ELECTRICAL AND ELECTRONICS L T P C

Course Outcomes Vs Program Outcomes Mapping:

DC CIRCUITS AND AC CIRCUITS

MEASURING INSTRUMENTS AND WIRING CIRCUITS

Course Outcomes Vs Program Outcomes Mapping:

1. To determine the acceleration due to gravity using Compound Pendulum

Course Outcomes Vs Program Outcomes Mapping:

ARRAYS AND FUNCTIONS

POINTERS, STRUCTURES AND FILES

1. Basic Unix Commands

ENGLISH FOR TECHNICAL COMMUNICATION II L T P C

Course Outcomes Vs Program Outcomes Mapping:

GRAMMAR AND VOCABULARY

Course Outcomes Vs Program Outcomes Mapping:

SEQUENCES AND SERIES

ANALYTIC FUNCTION AND CONFORMAL MAPPING

3. Venkataraman, M. K., Engineering Mathematics ‚ III A, The National Publishing