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    McFarland Standard

    Uploaded by

    Jean Paul
    The McFarland Equivalence Standards are used to standardize the density of bacterial suspensions for identification and susceptibility testing. They contain latex beads suspended in a buffer solution at concentrations corresponding to turbidity standards of 0.5, 1.0, 2.0, 3.0, and 4.0. Bacterial suspensions adjusted to the same turbidity as a McFarland Standard will produce expected bacterial plate counts. The standards provide reliable and reproducible results when used according to instructions and stored properly.

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    McFarland Standard

    Uploaded by

    Jean Paul
    480 views1 page
    The McFarland Equivalence Standards are used to standardize the density of bacterial suspensions for identification and susceptibility testing. They contain latex beads suspended in a buffer solution at concentrations corresponding to turbidity standards of 0.5, 1.0, 2.0, 3.0, and 4.0. Bacterial suspensions adjusted to the same turbidity as a McFarland Standard will produce expected bacterial plate counts. The standards provide reliable and reproducible results when used according to instructions and stored properly.

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    McFarland Standard

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    The McFarland Equivalence Standards are used to standardize the density of bacterial suspensions for identification and susceptibility testing. They contain latex beads suspended in a buffer solution at concentrations corresponding to turbidity standards of 0.5, 1.0, 2.0, 3.0, and 4.0. Bacterial suspensions adjusted to the same turbidity as a McFarland Standard will produce expected bacterial plate counts. The standards provide reliable and reproducible results when used according to instructions and stored properly.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    480 views1 page

    McFarland Standard

    Uploaded by

    Jean Paul
    The McFarland Equivalence Standards are used to standardize the density of bacterial suspensions for identification and susceptibility testing. They contain latex beads suspended in a buffer solution at concentrations corresponding to turbidity standards of 0.5, 1.0, 2.0, 3.0, and 4.0. Bacterial suspensions adjusted to the same turbidity as a McFarland Standard will produce expected bacterial plate counts. The standards provide reliable and reproducible results when used according to instructions and stored properly.

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    © All Rights Reserved
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    of 1

    MCFARLAND STANDARDS

    INTENDED USE
    The McFarland Equivalence Standards are intended to be part
    of a quality control program for adjusting densities of bacterial
    suspensions that are used for identification and susceptibility
    testing. Each standard is made from different concentrations
    of latex beads mixed in a buffer liquid. The original McFarland
    Standards were made from the combination of Barium chloride and Sulfuric acid that result in a flocculate. Problems were
    encountered with this technique which included instability,
    storage, and reproducibility of the resulting suspension. These
    problems have been overcome by using latex particles in a buffer solution to make Colorimeter and McFarland Standards.
    SUMMARY AND EXPLANATION
    The McFarland Standard tubes contain latex particles suspended in a special buffer that are adjusted to an acceptable transmission range using a spectrophotometer at a wave length of
    either 600 or 625 nm. A bacterial suspension once adjusted to
    the same turbidity of a McFarland Standard produces expected
    bacterial plate counts and can be used in a variety of identification or susceptibility kits and methods.
    PRINCIPLE OF THE PROCEDURE
    The McFarland Equivalence Standards are used for adjusting
    densities of bacterial suspensions.
    MATERIALS PROVIDED
    Catalogue Number

    Description

    SD2350

    McFarland Standard Set - Containing


    one of each 0.5, 1.0, 2.0, 3.0, 4.0

    PRECAUTIONS
    1. Do not use product beyond the expiration date.
    2. Directions should be carefully read prior to use.
    3. The same size tube should be used in comparing bacterial
    suspensions to the McFarland Standards.
    TEST PROTOCOL
    1. Prior to use, gently invert the McFarland Equivalence
    Standard tube several times to assure uniformity of the suspension of latex particles.
    2. Adjust the turbidity of the log growth of the bacterial suspension to that of a known McFarland Equivalence Standard.
    3. Compare the turbidity by holding bacterial sample and
    McFarland Standard tubes up against the black and white
    bars printed on enclosed card.

    SAFETY
    See MSDS for additional information.
    REFERENCES
    1. McFarland, J., J.Amer.Med.Assoc. 14:1176, 1907.
    2. NCCLS Document, Performance Standards for Antimicrobial
    Disk Susceptibility Tests. 4th ed. 10:7, p 10, 1990.

    QUALITY CONTROL
    Each lot of McFarland Equivalence Standard Set is tested and
    results fall into a tight range of acceptability.
    INTERPRETATION OF RESULTS
    Equal disappearance or distortion of the black bar indicates a
    similar turbidity.
    McFarland Standard

    Approximate Cell Count


    Density (x108 cells)

    0.5

    1.5

    1.0

    3.0

    2.0

    6.0
    9.0
    12.0

    SD2300

    McFarland Standard 0.5

    3.0

    SD2301

    McFarland Standard 1.0

    4.0

    SD2302

    McFarland Standard 2.0

    SD2303

    McFarland Standard 3.0

    SD2304

    McFarland Standard 4.0

    STABILITY AND STORAGE


    All components should be stored 15-30C. Do not freeze.
    Components stored under these conditions will be stable until
    the expiration date shown on the label.

    PRODUCT CODE SD2350, SD2300, SD2301,


    SD2302, SD2303, SD2304

    LIMITATION OF THE PROCEDURE


    1. Coloured media may not provide the proper contrast with
    McFarland Equivalence Standards. Incorrect results will occur.
    2. Bacterial suspensions of older cultures may not compare to
    expected bacterial counts.
    3. These standards have been adjusted by a spectrophotometer. Use of any other instrumentation may not give reliable
    results.
    Revision: 2012 05

    Canada 800 268 2341 Fax 905 731 0206


    20 Mural Street, Unit #4, Richmond Hill, ON, L4B 1K3

    U.S.A. 800 522 7740 Fax 800 332 0450


    21 Cypress Blvd., Suite 1070, Round Rock, TX, 78665-1034

    U.K. 0151 353 1613 Fax 0151 353 1614


    3 Bassendale Road, Bromborough, Wirral, Merseyside, CH62 3QL

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