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    BD Facscanto Ii System Innovation With Impact: BD Biosciences Technical Specifications

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    BD Facscanto Ii System Innovation With Impact: BD Biosciences Technical Specifications

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    54 views4 pages

    BD Facscanto Ii System Innovation With Impact: BD Biosciences Technical Specifications

    Uploaded by

    neysaonline
    bd

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    BDBiosciences

    Technical Specifications

    BD FACSCanto II System
    Innovation with Impact
    Introduction
    The BD FACSCanto II system delivers flow cytometry data
    on an efficient, user friendly platform. Its flexible, modular
    design offers automation and up to eight-color capacity for
    rapid and easy production of high-quality data in clinical and
    research laboratories.
    Standard System
    6-color 2-laser system (488, 633 nm)
    Fully integrated fluidics cart
    BD FACSCanto clinical software
    BD FACSDiva software
    System Options
    8-color 3-laser system (405, 488, 633 nm)*
    8-color 2-laser system (488, 633 nm)*
    2D barcode reader option
    Choice of two automated sample loading systems with
    integrated covers for operator safety
    - BD FACS Loader with 40-tube capacity
    (also includes single-tube loading)
    - BD High Throughput Sampler for 96- and
    384-well microtiter plate capacity

    Specifications
    Installation Requirements
    Size (D x W x H)

    Cytometer 24 x 36 x 25 in.
    (61 x 91 x 64 cm)
    Fluidics cart 24 x 31 x 25 in.
    (61 x 79 x 64 cm)
    The cytometer depth increases to 30 in.
    (76 cm) with BD FACS
    Loader Option installed

    Weight

    Cytometer 320 lb (146 kg)


    Fluidics cart 112 lb (51 kg)

    Power

    100/115/230 VAC, 50 60Hz

    Operating Environment 16 30C, 20 80% non-condensing


    relative humidity
    Figure 1
    8-peak Rainbow beads

    Heat Dissipation with


    BD FACS Loader installed 1843 BTU/hr

    BDBiosciences
    bdbiosciences.com
    United States Canada
    Europe
    Japan
    Asia Pacific
    Latin America/Caribbean
    877.232.8995 888.259.0187 32.24.009.895 0120.8555.90 65.6861.0633 55.11.5185.9995
    For country-specific contact information, visit bdbiosciences.com/how_to_order/
    For In Vitro Diagnostic Use.
    Class I (1) Laser Product.
    Microsoft and Windows are registered trademarks of Microsoft Corporation.
    Alexa Fluor and Texas Red are registered trademarks and Pacific Blue is a trademark of Molecular Probes, Inc., Eugene, Or.
    BD, BD logo and all other trademarks are the property of Becton, Dickinson and Company. 2006 BD
    * Seven- and eight-color assays on this device require validation by the user for in vitro diagnostic use.
    Not validated for use with the BD Sample Prep Assistant II.
    For Research Use Only. Not for use in diagnostic and therapeutic procedures.
    Specifications are subject to availability and change.
    PatentsPE and APC: US 4,520,110; 4,859,582; 5,055,556; Europe 76,695; Canada 1,179,942;
    PerCP: US 4,876,190; Cy5.5 and Cy7: US 5,268,486; 5,486,616; 5,569,587; 5,569,766; 5,627,027;
    APC-Cy7: US 5,714,386; PE-Cy7: US 4,542,104
    23-8786-01

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    Optics

    Lymphocytes
    Monocytes
    Unstained Lymphocytes

    Lasers

    Air-cooled:
    488-nm solid state, 20-mW laser output
    633-nm HeNe, 17-mW laser output

    Laser Configuration

    Spatially separated beams with 9 x 65 m elliptical spots

    Optical Alignment
    Procedure

    Fixed
    No operator alignment required

    Flow Cell

    180 x 430 m rectangular quartz flow cell

    Collection Optics

    Optical-gel coupled 1.2 NA lens

    FSC Resolution

    1.0 m

    SSC Resolution

    0.5 m

    Fluorescence Detector
    Design

    Reflective optics with single transmission filter


    in front of each PMT

    FSC Detector

    Photodiode with 488/10 BP

    SSC Detector

    PMT with 488/10 BP

    Fluorescence Detectors

    6 PMTs in 4-2 standard configuration

    Blue Laser Dyes

    FITC, PE, PerCP or PerCP-Cy5.5, PE-Cy7


    (525, 575, 678 or 695, 785 nm)

    Red Laser Dyes

    APC, APC-Cy7 (660, 785 nm)

    Detector Bands

    Blue Laser: 530/30; 585/42; >670; 780/60 nm


    Red Laser: 660/20; 780/60 nm

    Fluorescence Threshold
    Sensitivities
    FITC <100 MESF; PE <50 MESF
    Sensitivity Measurement
    Using BD FACS
    7-Color Setup Beads







    Sensitivity determined with the setup beads measures the ability to


    resolve a dimly stained population from unstained cells. This
    sensitivity measure takes into account both the separation of the
    populations and the broadness of the negative population.
    Different fluorochromes give different separation of the stained
    and unstained populations; this is also taken into account in the
    sensitivity measurement. The higher the reported number, the
    higher the resolution (see Figure 2).
    Minimum values:
    FITC >15; PE >80; PerCP >9; PerCP-Cy5.5 >25; PE-Cy7 >120;
    APC >40; APC-Cy7 >16

    Filter Change Procedure Keyed filters, no tools required

    Fluidics
    General Operation

    Integrated fluidics cart and compressor with onboard housekeeping


    solutions for automated startup, shutdown, and cleaning cycles

    Sheath Consumption

    1.10 L/hr normal operation; <1 mL/hr standby

    Housekeeping Solution BD FACSFlow sheath solution 20 L


    Capacities
    BD FACSClean solution 5 L

    BD FACS shutdown solution 5 L

    Waste tank 10 L
    Carryover

    0.1%

    Sample Injection

    Direct into flow cell

    Max Particle Size

    50 m

    Sample Flow Rate, Min 10 L/min


    Sample Flow Rate, Max 120 L/min
    Figure 2
    Lysed whole blood gated on lymphocytes.
    Cytometer set up with sensitivity from
    BD FACS 7-color setup beads

    23-8786-01.indd 2

    Sample Acquisition Rate 10,000 events/sec, 6 compensated fluorescent parameters and 2


    scatter parameters

    12/8/06 11:40:32 AM

    Sample Dead Volume


    30 L
    (BD Falcon tubes 12 x 75 mm)

    System Cleaning

    Daily: Automated startup and shutdown procedures


    Monthly: Run long clean

    Data Management System


    Parameters

    Area (A), Width (W), Height (H) for all channels with up to
    2 ratios, and Time (T)

    Signal Processing

    18-bit dynamic range with IEEE 32-bit floating-point resolution

    Threshold

    Single parameter (any channel) or logical combinations of multiple


    parameters (any or all channels)

    Compensation

    Full inter-beam matrix, during or post acquisition

    Maximum Logical Gate


    Regions
    Limited only by system memory (2 GB RAM)
    CPU/Monitors

    HP Xw4300, with either 19" or 24" flat screen monitors

    Software

    BD FACSDiva v5.0.1, BD FACSCanto clinical v2.1

    Operating System

    Microsoft Windows XP Pro

    Cytometer Options
    8-Color Option with 3 Lasers
    Lasers

    Air-cooled:
    405-nm solid state diode, 30-mW fiber power output
    488-nm solid state, 20-mW laser output
    633-nm HeNe, 17-mW laser output

    Fluorescence Detectors

    8 PMTs in 4-2-2 configuration

    Laser Dyes

    Violet: Pacific Blue, AmCyan (455, 488 nm)


    Blue: FITC, PE, PerCP or PerCP-Cy5.5, PE-Cy7
    (525, 575, 678, or 695, 785 nm)
    Red: APC, APC-Cy7 (660, 785 nm)

    Detector Bands

    Violet: 450/50; 502 to 525 nm


    Blue: 530/30; 585/42; >670; 780/60 nm
    Red: 660/20; 780/60 nm

    8-Color Option with 2 Lasers


    Lasers

    Air-cooled:
    488-nm solid state, 20-mW output
    633-nm HeNe, 17-mW output

    Fluorescence Detectors

    8 PMTs in 5-3 configuration

    Laser Dyes

    Blue: FITC, PE, PE-Texas Red, PerCP or PerCP-Cy5.5, PE-Cy7


    (525, 575, 615, 678 or 695, 785 nm)
    Red: APC, Alexa Fluor 700, APC-Cy7
    (660, 720, 785 nm)

    Detector Bands

    Blue: 530/30; 585/42; 616/23; >670; 780/60 nm


    Red: 660/20; 712/21; 780/60 nm

    Figure 3
    Lysed whole human blood stained with
    CD4 antibody conjugates

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    Cytometer Options

    (continued)

    Sample Input with BD FACS Loader


    Loading

    40-tube carousel

    Sample/test ID

    Indexed carousel, with carousel ID barcode reader


    Worklist importable from BD FACS Sample Prep Assistant II

    Throughput

    56 min/carousel with BD Multi-Check high controls,


    66 min/carousel with BD Multi-Check low controls
    using BD Multitest 6-color TBNK application

    Miscellaneous

    Multiple clinical applications can be run on the same Loader carousel.

    Sample Input with BD High Throughput Sampler


    (see BD HTS Specification Sheet for more details)
    Loading

    96- and 384-well microtiter plates

    Throughput

    <15 min/96-well plate in high-throughput mode with 2-sec


    acquisition

    Carryover

    1%

    Barcode Reader with Stand


    Figure 4
    CENs stained with propidium iodide (top) and
    Hoechst 33258 dye (bottom)

    Use with

    BD FACSCanto clinical software

    2D Reader

    Streamlined input of BD FACS 7-color setup bead target values,


    input of patient information

    Figure 5
    E. coli bacterium. Pecan pollen (45 to 55 m).
    K562 cells.

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