Purification of Dna Using Silica Technology: A Dissertation ON
Purification of Dna Using Silica Technology: A Dissertation ON
Purification of Dna Using Silica Technology: A Dissertation ON
DISSERTATION
ON
PURIFICATION OF DNA USING SILICA TECHNOLOGY
Submitted to
SHRI GURU RAM RAI INSTITUTE OF TECHNOLOGY AND SCIENCE
Dehradun
Affiliated to
Hemwati Nandan Bahuguna Garhwal University Srinagar,
(A CENTRAL UNIVERSITY)
I N PARTI AL FULFI LMENT FOR THE DEGREE
Of
MASTER OF SCIENCE
(BI OTECHNOLOGY)
2012-2014
Under the Supervision:-
Sr. Asst. Professor
Dr.Rahul kumar
Division of Life Sciences
Submitted by
Rifa Sultana
M. Sc. Biotechnology
Enrollment No-G12535195
Batch:-2012-2014
Shri Guru Ram Rai Institute of Technology And Science, Patel Nagar, Dehradun,
Uttarakhand.Ph.Nos:01352721763, 2726435,
2726209.Fax:01352721762.Website: www.sgrrits.org.
Dr.Manoj Gahlot
Principal
Division of Life Sciences
FORWARDING
With immense pleasure I feel honour in certifying and forwarding the
Dissertation entitled PURIFICATION OF DNA USING SILICA TECHNOLOGY
submitted by Rifa Sultana M.Sc. Biotechnology, IV semester for the award of
degree in the partial fulfillment Master of Science in Biotechnology from Hemwati
Nandan Bahuguna Garhwal University,(A central University) Srinagar, Garhwal,
Uttarakhand, is carried out under the supervision and guidance of Dr. Anant Rai,
Institiute of Biotechnology and IT, Barelly and Dr. Rahul Kumar, Asst, Professor,
Department of Biotechnology, Division of Life Sciences during the current
academic session.
Dated Dr. Manoj Gahlot
Principal
Division of Life Sciences
SGGRITS, Dehradun
Shri Guru Ram Rai Institute of Technology And Science, Patel Nagar, Dehradun,
Uttarakhand.Ph.Nos:01352721763, 2726435,
2726209.Fax:01352721762.Website: www.sgrrits.org.
Shri Guru Ram Rai Institute of
Technology and Science, Patel
Nagar, Dehradun, Uttarakhand,
248001
Dr. Ruhal Kumar
Assistant Professor
Division of Life Sciences
Shri Guru Ram Rai Institute of Technology & Science
Patel Nagar, Dehradun 2480001
INTERNAL SUPERVISOR CERTIFICATE
This is to certify that Ms. Rifa Sultana, student of M.Sc. Biotechnology, IV
Semester, (Enrolment Number: G12535195), Shri Guru Ram Rai Institute of
Technology and Science, Patel Nagar, Dehradun, (Affiliated to H.N.B. Garhwal
University, Srinagar Garhwal, Uttrakhand), has successfully completed her project
work/Dissertation entitled PURIFICATION OF DNA USING SILICA
TECHNOLOGY. under my supervison.
During the course of project work her performance and coonduct was
excellent. I wish her all the best for future endeavours.
(Dr. Rahul Kumar)
CANDIDATES DECLARATION
I hereby declare that the Thesis Entitled PURIFICATION OF DNA USING
SILICA GEL TECHNOLOGY is submitted to SHRI GURU RAM RAI INSTITUTE
OF TECHNOLOGY AND SCIENCE towards the partial fulfillment of the
requirement for the award of degree of M.Sc. Biotechnology, from H.N.B Garhwal
University,Srinagar,Garhwal(Uttarakhand) under the supervision and guidance of
Dr. Anant Rai, Institiute of Biotechnology and IT, Barellly and Dr.Rakesh Rai
Sr.Asst.Prof,Shri Guru Ram Rai Institute Of Technology and Science, Patel
Nagar,Dehradun,Uttarakhand.
The thesis has not formed the basis of the award of any other degree or
similar title to any candidate of any university/institution.
Date:
Place: Rifa Sultana
M.Sc Biotechnology (4
th
Sem)
Batch: 2012-2014
Shri Guru Ram Rai Institute of Technology And Science, Patel Nagar, Dehradun,
Uttarakhand.Ph.Nos:01352721763, 2726435,
2726209.Fax:01352721762.Website:www.sgrrits.org.
ACKNOWLEDGEMENT
I would like to express my profound sense of gratitude towards Dr.
Manoj Gahlot Principal, Division of Life Sciences, Shri Guru Ram Rai
Institute of Technology & Science providing for me an opportunity to
improve my learning.
I offer my humble regards to my Internal Supervisor Dr. Rahul
Kumar Asst. Professor, Division of Life Sciences, Shri Guru Ram Rai
Institute of Technology and Science, Patel Nagar, Dehradun,
Uttarakhand for the continuous supervision and guidance during the
course of present work.
I find immense pleasure in expressing my gratitude towards my
External Supervisor Dr. Anant Rai, Institiute of Biotechnology and IT
for allowing me to complete my project from my desired & convenient
place.
I am thankful to all faculty members and non teaching staff of the
Division of Life Sciences SGRRITS for encouraging & supporting me in
every possible way
I am also thankful to my friend and classmate Irfan Rashid for his
for his support and encouragement without which it was very hard to
achieve.
I shall be failing in moral duties, if I miss expressing deep sense of
gratitude to my parents for their blessings, wishes and various kinds of
support during the entire project work.
I am heartly thankful to the GOD for blessings and favour to me.
(Rifa Sultana)
LIST OF ABBEVATIONS
S.NO ABBEVATIONS FULL FORM OF ABBEVATION
1 EMC Equilibrium Moisture Content
2 RH Relative humidity
3 Gm Gram
4 m/g Milli per gram
5 % Percentage
6 Mm Millimeter
7 & And
8 Kb Kilobyte
9 PCR Polymerase chain reaction
10 RAPD Random Amplified Polymorphic DNA
11 RFLP Restriction Fragment Length Polymorphism
12 AFLP Amplified Fragment Length Polymorphism
13 l Micro liter
14 < Less than
15 Da Dalton
16 Dt Metric tonne
17 HPMC Hydroxypropyl methycellulose
18 Bp Basepair
LIST OF TABLES
S.NO NAMES OF TABLES
1 Names of the equipments used
2 Names of the glassware used
3 Names of the chemical used
4 Composition of extraction buffer
5 Names of the chemicals
LIST OF THE PHOPLATES
S.NO NAMES OF THE PHOTOPLATES
1 Isolation of DNA
2 DNA bands on the agrose gel
S.NO
TOPICS
1 INTRODUCTION
2 REVIEW OF LITERATURE
3 OBJECTIVES
4 MATERIALS AND METHODS
4.1 Extraction of Genomic DNA
4.2 Separation of DNA sample on Agrose Gel electrophoresis
4.3 Mixing of DNA
4.4 Loading of DNA
4.5 Running Gel
4.6 Purification of DNA through Silica technology
5 RESULTS
5.1 Collection of plant sample
5.2 Isolation of Genomic DNA from the leaves of sample
5.3 Separation of DNA using Gel electrophoresis
5.4 Purification of DNA sample Silica technology
6 SUMMARY
7 REFRANCES