Murashige and Skoog Medium

Plant Cell, Tissue and Organ Culture 67: 89–92, 2001. © 2001 Kluwer Academic Publishers. Printed in the Netherlands. ... Micropropagation of the nickel hyperaccumulator, Hybanthus floribundus (Family Violaceae) ... SD Bidwell1, JW Pederick1, J. Sommer-Knudsen1,2 & IE ...

The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Afterward, both the control and treated samples were analyzed using various analytical techniques. The X-ray diffraction (XRD) analysis showed 19.92% decrease in the crystallite size of treated sample with respect to the control. The thermogravimetric analysis (TGA) showed the increase in onset temperature of thermal degradation (Tonset) by 9.41% and 10.69% in first and second steps of thermal degradation, respectively after the biofield energy treatment as compared to the control. Likewise, Tmax (maximum thermal degradation temperature) was increased by 17.43% and 28.61% correspondingly in the first and second step of thermal degradation in the treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis indicated the 143.51% increase in the latent heat of fusion of the treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated MS media showed the alteration in the frequency such as 3165→3130 cm-1 (aromatic C-H stretching); 2813→2775 cm-1 (aliphatic C-H stretching); 1145→1137 cm-1 (C-N stretching), 995→1001 cm-1 (S=O stretching), etc. in the treated sample with respect to the control. The UV spectra of control and treated MS media showed the similar absorbance maxima (λmax) i.e. at 201 and 198 nm, respectively. The XRD, TGA-DTG, DSC, and FT-IR results suggested that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of the MS media. As a result, the treated MS media could be more stable than the control, and might be used as better media in the plant tissue culture technique.

Jatropha curcas L. is among important tree crops in the world with a potential for biofuel production. In Ethiopia, there is a soaring investors' interest to produce Jatropha in the country for biodiesel production. However, insufficient good quality propagation material is a major production constraint. A study was undertaken to establish a protocol for in vitro mass propagation of three Ethiopian Jatropha accessions viz. Metema, Adami Tulu and Shewa Robit through direct organogenesis from nodal explant. The result revealed that the highest percentage of shoot induction (86-90%) was achieved on MS medium with BAP (1 mg/L) and IBA (0.5 mg/L) for all the three accessions. The maximum number of shoots (6) was obtained for Metema when BAP (0.5 mg/L) with Kn (0.5 mg/L) was used. Whereas, the maximum (3.2 cm) shoot length was recorded for Shewa Robit on media with 0.5 mg/L Kn. The highest rooting percentage (84.8-88%) and maximum root number (5.43) were recorded on media supplemented with 0.25 mg/L IBA. Shewa Robit and Metema had longer roots on media with 0.25 mg/L IBA. Finally, the plantlets were successfully established in greenhouse with survival rate of 86.67% for Shewa Robit followed by 73.33 and 66.67% for Metema and Adami Tulu, respectively. This study provided optimal protocol for micro-propagation of Jatropha accessions through direct organogenesis to boost its production.