ECV

El siguiente artículo comprende la metodología y los principales detalles técnicos con los cuales se evaluaron las posibles herramientas tecnológicas de apoyo (i.e Amplificador de señales EEG, Electroestimulador FES) a la discapacidad. Se realizó un estudio exploratorio de personas con ECV (Enfermedad Cerebro Vascular) en la ciudad de Cali, Colombia. Las herramientas de apoyo sirvieron para evaluar el potencial inicial de las mismas en los procesos de rehabilitación de las personas con hemiparesia. Las pruebas realizadas contemplaron un análisis de nuevos clasificadores del procesamiento de la señal, a fin de empezar a mejorar la interfaz visual y realimentación de estas herramientas de apoyo y hacer más eficiente la incidencia neuromotora sobre la tarea de rehabilitación

Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated spatiotemporal organization of telomeres in living human ECV-304 cells stably expressing telomere binding proteins TRF1 and TRF2 fused to mCitrine using four dimensional microscopy. We thereby made use of controlled light exposure microscopy (CLEM), a novel technology that strongly reduces photodamage by limiting excitation in parts of the image where full exposure is not needed. We found that telomeres share small territories where they dynamically associate. These territories are preferentially positioned at the interface of chromatin domains. TRF1 and TRF2 are abundantly present in these territories but not firmly bound. At the onset of mitosis, the bulk of TRF protein dissociates from telomere regions, territories disintegrate and individual telomeres become faintly visible. The combination of stable cell lines, CLEM and cytometry proved essential in providing novel insights in compartment-based nuclear organization and may serve as a model approach for investigating telomere-driven genome-instability and studying long-term nuclear dynamics. ' 2008 International Society for Advancement of Cytometry Key terms telomere; TRF1; TRF2; nuclear organization; chromatin dynamics; CLEM; live cell imaging TELOMERES are the natural ends of linear chromosomes. A mammalian telomere consists of a double stranded array of simple TTAGGG repeats ending in a single stranded overhang that folds back to form a T-loop structure (1). In combination with sufficient telomere repeats, a complex of indirect and direct telomere binding proteins, dubbed shelterin, assures proper telomere structure and function. The specificity of shelterin for telomeric DNA is due to the recognition of TTAGGG repeats by three of its components: the homodimers telomeric repeat binding factor 1 and 2 (TRF1 and TRF2) that bind the duplex part of telomeres, and protection of telomeres 1 that binds to the single stranded TTAGGG repeats present at the three-overhang and in the D loop of the T-loop configuration (2-5).

El siguiente artículo comprende la metodología y los principales detalles técnicos con los cuales se evaluaron las posibles herramientas tecnológicas de apoyo (i.e Amplificador de señales EEG, Electroestimulador FES) a la discapacidad. Se realizó un estudio exploratorio de personas con ECV (Enfermedad Cerebro Vascular) en la ciudad de Cali, Colombia. Las herramientas de apoyo sirvieron para evaluar el potencial inicial de las mismas en los procesos de rehabilitación de las personas con hemiparesia. Las pruebas realizadas contemplaron un análisis de nuevos clasificadores del procesamiento de la señal, a fin de empezar a mejorar la interfaz visual y realimentación de estas herramientas de apoyo y hacer más eficiente la incidencia neuromotora sobre la tarea de rehabilitacion. .

Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated spatiotemporal organization of telomeres in living human ECV-304 cells stably expressing telomere binding proteins TRF1 and TRF2 fused to mCitrine using four dimensional microscopy. We thereby made use of controlled light exposure microscopy (CLEM), a novel technology that strongly reduces photodamage by limiting excitation in parts of the image where full exposure is not needed. We found that telomeres share small territories where they dynamically associate. These territories are preferentially positioned at the interface of chromatin domains. TRF1 and TRF2 are abundantly present in these territories but not firmly bound. At the onset of mitosis, the bulk of TRF protein dissociates from telomere regions, territories disintegrate and individual telomeres become faintly visible. The combination of stable cell lines, CLEM and cytometry proved essential in providing novel insights in compartment-based nuclear organization and may serve as a model approach for investigating telomere-driven genome-instability and studying long-term nuclear dynamics.