Clinical Laboratory

The study focused on the main laboratory department of the East Avenue Medical Center, a 600-bed tertiary government hospital. An overview of the entire hospital organization and related departments was first made to put the study into context. Staffing, scheduling, workflow of people, supplies, and waste were also studied to see how well the facility was suited to the needs of the occupants and the hospital. The various sections and their corresponding functions and equipment were tabulated and classified according to risk level. The study concluded by showing the department’s good practices in space planning, layout, and workflow, and by providing recommendations for the improvement of the facility.

Co-written with Dr Ramli MD Razis, Gemma P Ramos, Dr Mazila Mohd Zain, and Dr Abdul Kadir Muhammad Shauqi

This Lab aims at:

1. The clinical consolidation of the Quanto-Geometric Psychophysics Framework of the Human Mental thru the establishment of a data acquisition and experimental platform.

2. The eventual development of mental health and clinical practice products arising from the visualizations, methodologies and solutions brought about by the Theory.

The sum of the Theory is exposed in the publication Functional Architecture of the Human Mental, ISBN 978-0-9647466-8-8, co-authored by Joseph J. Jean-Claude, Laser-Optics Associate Engineer, and Caroline Stamu-O’Brien, MD.

...continued in statutory document (please download below)...

Methods: 40 patients with adenomas and 40 controls were enrolled. Body weight, height, waist circumference, and blood pressure were recorded. Fasting plasma glucose, lipids, C-reactive protein, and adiponectin levels were measured. Metabolic Syndrome was defined and lifestyle characteristics assessed. Results: No differences were found in adiponectin values between patients and controls (p = 0.101). Adiponectin levels were significantly higher in females than in males (p = 0.004). Adiponectin levels did not result in significant association with colorectal adenomas even after adjustment for metabolic and life style parameters. Conclusions: This study did not confirm the hypothesis that high levels of adiponectin confer decreased risk of colorectal adenomas. (Clin. Lab. 2013;59:211-214.

Summary  We used a new and remarkably simple method to examine the extent of in vivo lipoprotein glycation in type II diabetic patients with atherosclerosis and diabetic patients with no complications. Serum glycated lipoprotein levels were determined by agarose gel film electrophoresis in 48 non-diabetic control subjects and 39 diabetic patients, of whom 26 had no complications and 13 had atherosclerotic

Simple, quick guide and note collection, allowing complementary medicine students and beginners to grasp the basics of Complete Blood Count laboratory analysis and Hematology. Information is presented, summarized and explained in a concise way, accompanied by easily understandable images and graphs.

The guide also contains notes on relevant Chinese medicine peer-reviewed research, as well as western medical and scientific papers and sources making it a perfect source, since it is not exclusively Chinese medicine-oriented, and can be useful to complementary medicine students and practitioners from various and distinct fields of research.

A useful starter's guide and research source, for both students and complementary medicine practitioners.

cek darah lengkap,cek darah rutin,cek darah dbd,cek darah demam berdarah,cek darah normal

Pemeriksaan kesehatan sangatlah penting untuk mencegah dan mendeteksi penyakit secara dini. Gaya hidup dinamis, stres, atau riwayat kesehatan bisa punya dampak jangka-panjang bagi kesehatan kita. Banyak kondisi medis tak bisa terlihat oleh mata awam. Oleh sebab itu, anda perlu pemeriksaan laboratorium untuk mendeteksi masalah kesehatan anda.
Para profesional kami siap membantu anda untuk mengetahui dan mengerti kondisi kesehatan anda. Kami menyediakan beragam paket pemeriksaan kesehatan

Lab Parahita, Surabaya, 031-502 0552

С Т А Н Д А Р Т И 48 К аждый 5-й трудоспособный человек страдает от боли. Основной функцией боли является защита организма. С точки зрения эволюции, если бы человек не чувствовал боли (например, укус змеи, термический ожог и др.), то люди просто бы не выжили. Существует редкое тяжелое генетическое заболевание -врожденная анальгезия, при которой человек не чувствует боли. Сейчас в мире от этого недуга страдает несколько сотен человек. Известен также «синдром Марсили» с уникальным механизмом наследования, при котором от поколения к поколению переходит нечувствительность к боли. Раньше такие люди вообще не выживали, а сейчас они находятся под наблюдением врачей. Однако даже в таких условиях выживают немногие, так как боль сигнализирует не только о внешних, но и внутренних нарушениях (заболевания сердца, аппендицит и др.). Комплексным изучением проблем боли занимается такая отрасль медицины, как алгология, которая находится на стыке научных дисциплин, имеющих непосредственное отношение к боли -травматология, анестезиология, онкология, нейрохирургия, неврология, психиатрия, эпидемиология и др.

Газета «Новости медицины и фармации» Гастроэнтерология. Проктология (649) 2018 (тематический номер) Вернуться к номеру Молекулярно-генетическая диагностика синдрома Жильбера Авторы: Мельник А.А. -к.б.н. Рубрики: Гастроэнтерология Разделы: Справочник специалиста

This is an excerpt from: Chiropractic Patient Assessment Laboratory Interpretation and Risk Management: Introduction to Inflammation Mastery and Functional Inflammology (Volume 1) Paperback – July 29, 2014 https://www.amazon.com/dp/1500686379. To keep the printing and sales cost down to increase availability especially to students and recent graduates, the 2014 book was printed in black-white-grayscale. The excerpt provided here is of identical information (Chapter 1) sampled from the full-color 2016 printing of Inflammation Mastery 4th Edition.

This review reports the characteristics of the human surface molecule CD38, a structure not linked to a definite line and predominantly expressed in early and activated phenotypes. The CD38 molecule consists of a single chain of 46 kDa, spanning the membrane and with the carboxyl terminus located in the extracellular compartment. The CD38 molecule is also involved in the transduction of activation and proliferation signals, which are line unrestricted. The gene coding for the CD38 antigen has been cloned and used for the construction of simian and mouse transfectants expressing the human molecule. These cell models are used for the analysis of several unanswered issues, mainly concerning the in vivo function of CD38, the existence of a natural ligand and of polymorphism in the population.

Objective: This study was done to assess the accuracy of Accutrend GCT meter in determining blood cholesterol levels in our setup, with an aim to recommend its use in clinical practice
Methods: This cross sectional study was conducted on 251 healthy volunteers from April to May 2013. Capillary whole blood samples were obtained using sterile lancets and cholesterol levels were measured using Accutrend GCT meter (Roche Diagnostics, Mannheim, Germany) using the standard technique. Three ml whole blood was also collected in plain tubes by the same operator without applying a tourniquet. Serum cholesterol levels were determined using Merck Microlab 300 Automated Clinical Chemistry Analyser the same day. The laboratory technician performing this test was blinded to results obtained with Accutrend GCT meter. The seven patients with readings beyond the analytic range of the Accutrend GCT meter were excluded, meaning that data analysis was performed on 244 results only.
Results: Results of 182 males and 62 females having a mean age of 38.54± 15.25 years were analysed. There was a significant difference in mean cholesterol levels measured by Accutrend GCT and Microlab-300 (4.73± 0.74mmol/l and 4.67± 0.86 mmol/l; p=0.021), producing a measurement bias of 1.43% with Accutrend GCT meter. A strong positive correlation was found between the results of the two techniques (r=0.852 ,p<0.001).
Conclusions: Accutrend GCT meter provides accurate measurement of blood cholesterol levels and its use should be encouraged for point-of-care measurements.

Progress has been made in understanding glomerulonephritis but the answers to many key questions still elude us. It appears that glomerular damage arises from immune events which result from an interaction between a susceptible individual and some environmental agents, usually an infection. In the developed world the role of the genetic constitution is predominant, whilst in the Third world the overwhelming exposure to pathogens may be crucial. Immune complex formation undoubtedly plays a major role in the initiation of glomerular injury, but deposition of soluble preformed complexes within the glomerulus now seems to be only one mechanism,in situ combination of fixed antigen and free antibody, and insoluble (precipitating) complexes both playing a part as well. New understanding of modes of damage to the glomerulus strongly implicates monocytes as injurious agents in both experimental and human nephritis, and platelets may also play a role in amplifying injury. One of the main questions is why only some forms of glomerulonephritis in only some patients progress to renal failure. Whilst continuing exposure to the antigen initiating the nephritis may be important, secondary mechanisms involving autologous antigens may be important. Further, non-immunologic mechanisms which arise from hyperperfusion of glomerular capillaries appear capable of damaging the glomerulus, and both this mechanism and proteinuria itself appear capable of inducing glomerulosclerosis. The role these non-immunologic mechanisms play may be the reason for our failure, in general, to influence the course of glomerulonephritis by manoeuvres designed to inhibit immunologic injury.

Background: A correct preanalytical phase procedure is critical to get an adequate sample and consequently to achieve the most reliable laboratory results, promoting patient safety. Continuous laboratory staff changes create the need to establish improvement strategies to reduce the error risk. The objective was to show how the numbers of preanalytical errors related to unsuitable samples in a hospital setting decrease following two improvement strategies related to new technology and educational actions and how their effects were measured by monitoring indicators. Methods: Samples were drawn by the laboratory and other hospital departments' nurses without previous patient appointment, therefore, prior tube preparation was not available before the phlebotomy. Corrective measures for these activities were established: educational program for nurses and a system of custom labels, which correlate each laboratory test in the Laboratory Information System (LIS) with the corresponding tube. Three phases were defined based on the implementation dates of the improvement actions to be assessed. The set of indicators designed to monitor the improvement related to clotted, hemolyzed, insufficient, and uncollected samples. Data were collected and indicators calculated from the LIS using a data warehouse application. Patient satisfaction with respect to phlebotomy was also measured annually using a scoring survey. Results: There was a reduction in all types of preanalytical sample errors related to the improvement strategies adopted. The indicators demonstrated that the unavailable, insufficient, and clotted samples decreased between two-and threefold , whereas hemolysis errors benefited more from these improvement strategies. Patient satisfaction with the laboratory and phlebotomy procedures improved over the past several years as based on the annual satisfaction surveys. Conclusions: The educational program for nursing personnel is relevant and important as can be seen in the decrease of sample errors and the resulting quality improvement. The custom label system minimizes the potential oversight of forgetting to draw a tube, which happens frequently when operating without appointments, by printing the labels according to requested tests. Detection, identification, and monitoring of the error and implementing strategies to improve preanalytical quality reduces error numbers and thereby improves patient safety and health system outcomes.

A 64-year-old female presented with poorly differentiated acute myeloid leukemia (AML) following a history of essential thrombocythemia. Bone marrow showed hypercellularity and 21% blasts, which were CD13 1 , CD33 1 , CD34 1 , CD45 dim , MPO 1 , CD117 1 , and HLADR 1. Cytogenetic testing revealed a complex karyotype; del(5q) and del(7q) were identified by fluorescence in situ hybridization. Blasts were seen on peripheral smear review. After failed induction with arabinosylcytosine and daunorubicin, treatment was replaced with decitabine and ruxolitinib. Complications included (presumed) fungal pneumonia, pleural and pericardial effusions, Staphylococcus bacteremia, pathological fracture of right coracoid process, and widespread osteolytic lesions. The patient reported fevers, night sweats, mild cough, and exertional dyspnea. Thoracentesis was performed, and the following cell counts were obtained: white blood cells, 1450/mL; red blood cells, 330/mL; and differential, 64% blasts with immunophenotype consistent with the initial diagnosis. As shown in panels A and B, blasts were pleomorphic with medium to giant/irregular forms, variable N:C ratio, basophilic cytoplasm, conspicuous nucleoli, smooth chromatin (moderate clumping in some), and occasional multinucleated form. Few blasts had sparse azurophilic granules, and a large atypical hyperchromatic mesothelial cell was also noted on the slide (panel A, top center). Cytospin-induced changes in blast morphology include irregular nuclear contours, more pronounced nucleoli, increased cytoplasm, and abnormal concentration of cytoplasmic granules. Cytospin smears should be evaluated with caution in light of these in vitro preparation–induced changes. For additional images, visit the ASH IMAGE BANK, a reference and teaching tool that is continually updated with new atlas and case study images. For more information visit http://imagebank.hematology.org.

Современные подходы к исследованию системы гемостаза системы гемостаза Мельник Александр Александрович, канд.биол.наук Мельник Александр Александрович, канд.биол.наук г.Киев -2015 г. г.Киев -2015 г. Цель -рассказать просто о сложном Цель -рассказать просто о сложном Фильм «Гемостаз. Коагуляция и фибринолиз» (щелкнуть на экран) Биологическая система, обеспечивающая, с одной Биологическая система, обеспечивающая, с одной стороны, сохранение жидкого состояния крови и стороны, сохранение жидкого состояния крови и достаточное кровоснабнабжение органов, а с достаточное кровоснабнабжение органов, а с другой, -предупреждение и быструю остановку другой, -предупреждение и быструю остановку кровотечений. кровотечений. Haima + Stasis Нитью фибрина начинается (тромбирование пупочных сосудов) Нитью фибрина начинается (тромбирование пупочных сосудов) и кончается жизнь и кончается жизнь Основные компоненты системы гемостаза: Основные компоненты системы гемостаза:

5. Examineaza sedimentul urinar prin metoda Neciporenco. Se bazeaza pe calculare elementelor celulare(eritrocitele, leucocitele si cilindrii) in 1 ml de urina proaspata eliminate, cu ajutorul camerei de calcul cu recalcularea rezultatelor in 1 l de urina. In laboratorul imediat se determina reactia ei, fiindca in urina cu reactia alcalina poate avea loc lezarea partial a elementelor de aceea se va examina cit mai repede. Tehnica 1. Intr-o eprubeta de centrifugare se pipeteaza 10 ml de urina si se supune centrifugarii 3500 rot/min timp de 3 min. 2. Cu ajutorul unei pipete cu para de cauciuc se inlatura centrifugatul , lasindu-se exact 0,5 ml de sediment. 3. Daca sedimentul e ambundent se lasa 1 ml. 4. Se amesteca si se umple camera de calcul Goreaev sau Fux-Rozental. 5. Se Numara elementele aparte pe toata reteaua Goreaev sau Fux-Rozental cu ocularul X7, obiectivul X40. Calcularea se efectueaza dupa formula:

An airlock is a transitional space that typically has two doors in series to separate a
controlled environment (such as cleanroom, lab, operating room, or isolation room)
from a corridor, or vice versa. The two doors should be interlocked to avoid being
opened at the same time. An airlock area is often ventilated with filtered supply air,
with return or exhaust air to exit. However, the relative air volume for supply, return
or exhaust could vary based on the type of airlock. Poor airlock design concepts may
cause undesired migration of chemical fumes, particles, or microbiological agents,
which can be toxic, harmful, or infectious, into protective areas, or cause contaminated products or processes from excessive airborne contaminants inside the
controlled environments.

С Т А Н Д А Р Т И 36 П о данным Всемирной организации здравоохранения (ВОЗ), от инсульта в мире ежегодно умираЮт около 6,3 миллиона человек (10,8 % от общего числа смертей). Летальность при этой патологии занимает второе место, уступая лишь заболеваниям сердечно-сосудистой системы и составляет 8 % среди мужчин, 16 % среди женщин и показателем инвалидизации 3,2 на 1 тыс. населения из которых 1/3 являются лицами трудоспособного возраста. При этом отмечается «омоложение» инсульта с увеличением его распространенности среди людей трудоспособного возраста. Последствия инсульта носят не только медико-социальный, но и экономический характер. Например, в Англии на лечение и реабилитацию больного, перенесшего инсульт, расходуется в среднем 22 000 фунтов стерлингов в год.

The classic technique of PD consists of the en-bloc removal of the distal segment of the stomach (antrum), the first and the second portions of the duodenum, specifically the head of the pancreas, the distal CBD, and the gallbladder. Another approach to this procedure is known as the pylorus-sparing PD. In this approach, a small segment of the duo denum is left in situ with the entire stomach to preserve the pylorus and prevent the postgastrectomy-related symptoms and complications. The classic Whipple and the pylorus-preserving operations are associated with comparable operation times, blood loss, hospital stays, mortality, morbidity, and the incidence of delayed gastric emptying (Mathur et al., 2015). The overall long-term and the disease-free survival is comparable in both groups.

Газета «Новости медицины и фармации» №8 (695), 2019 Лабораторная диагностика тромботической микроангиопатии Авторы: Мельник А.А., к.б.н., руководитель проекта специализированного медицинского центра «Оптима-фарм», г. Киев, Украина Разделы: Справочник специалиста Резюме Тромботическая микроангиопатия (ТМА) -это патологический процесс, сопровождающийся системным тромбозом вследствие окклюзии сосудов микроциркуляторного русла (артериол, капилляров) различных органов, который приводит к микроангиопатической гемолитической анемии (МАГА), тромбоцитопении и повреждению органов [1-3]. ТМА классифицируют на первичные и вторичные. Первичные ТМА включают в себя тромботическую тромбоцитопеническую пурпуру (ТТП), типичный гемолитико-уремический (тГУС) и атипичный синдром (аГУС). К многочисленным вторичным ТМА относятся преэклампсия, эклампсия, HELLPсиндром, аутоиммунные заболевания, злокачественные опухоли, вирус иммунодефицита человека, грипп H1N1, гломерулопатии, метилмалоновая ацидурия с гомоцистеинурией, некоторые лекарственные препараты, ионизирующее излучение, трансплантация солидных органов и костного мозга (рис. 1).

In laboratories where dangerous conditions might exist, safety precautions are important. Rules exist to minimize the individual's risk, and safety equipment is used to protect the lab user from injury or to assist in responding to an emergency. The purpose of the research is to create an awareness of university students on laboratory safety. After a thorough survey, the following aspects were identified and are briefly discussed here is rule and regulations, safety and role of Occupational Safety, Health and Environment (OSHE) in the laboratory. Based on the survey done from 30 respondents, the awareness of students on laboratory safety is good but there are some students not following the rules in the laboratory.

Липиды -это группа органических молекул, входящие в состав всех живых клеток. Они включают жиры, воски, стероиды, жирорастворимые витамины, триглицериды, фосфолипиды и др. Основными физиологическими функциями липидов являются структурная, энергетическая, защитная и регуляторная. Липидный обмен, происходящий в клетках, представляет собой сложный биохимический и физиологический процесс. Целый ряд заболеваний обусловлен нарушением липидного обмена или дислипопротеинемией. Дислипопротеинемии -это наследственные или приобретенные состояния, характеризующиеся нарушением синтеза, катаболизма, удаления из циркуляции липидов и липопротеинов, что приводит к нарушению их содержания в крови (снижение или повышение) или появлению патологических липопротеинов. Дислипопротеинемии служат основой для сердечно-сосудистых заболеваний (ССЗ), инсультов, ряда форм артериальной гипертензии и других болезней. Патология липидного обмена находится в фокусе постоянного внимания не только кардиологов, невропатологов, терапевтов, семейных врачей, геронтологов, но также и педиатров (существует не менее десятка наследственных сфинголипидозов, нарушающих раннее психомоторное развитие детей, а около 10% детей в экономически благополучных странах имеют избыточный вес и их число возрастает). Все это свидетельствует о том, что необходимость знаний о патологии липидного обмена требуется врачу любой специальности. По данным Всемирной Организации Здравоохранения (ВОЗ) не менее 10% населения в мире (Европа и Северная Америка -более 20%) страдают какой-либо дислипопротеинемий. Нарушение липидного обмена приводит к развитию атеросклероза. Атеросклероз -системное поражение артерий крупного и среднего калибра, сопровождающеееся накоплением липидов, разрастанием фиброзных волокон, дисфункцией эндотелия сосудистой стенки, приводящее к местным и общим расстройствам гемодинамики. Атеросклероз являться патоморфологической основой ССЗ, ишемического инсульта, облитери-рующего поражения нижних конечностей и др. В последние годы заболеваемость атероскле-розом в Украине приобрела угрожающие масштабы, опередив по риску развития потери работоспособности, инвалидизации и смертности такие причины как травмы, инфекционные и онкологические заболевания. Причинами атеросклероза являются дислипопротеинемии, наследственная предрасположенность, сахарный диабет, излишнее употребление в пищу животных жиров, курение, артериальная гипертензия, алиментарное ожирение. С наибольшей частотой атеросклероз поражает мужчин старше 45-50 лет (в 3-4 раза чаще, чем женщин), но встречается у пациентов более молодого возраста. Атеросклероз является одной из самых главных факторов в процессе развитии проблем с кровоснабжением жизненно важных органов человека, прежде всего сердца и головного мозга, что приводит к инфаркту и инсульту. Ежегодно в Украине фиксируется 50 тыс. инфарктов миокарда и около 120 тыс. инсультов. Восстановление прежней трудоспособности после мозгового инсульта у большинства людей проблематично. Только 10-20% возвращаются к труду, из них около 8% сохраняют свою профессиональную пригодность, а 25% нуждаются в посторонней помощи. К концу года после перенесенного инсульта у 25-30% больных развивается деменция. Среди 35% украинцев, у которых регистрируется инсульт, являются работоспособными (не достигшими пенсионного возраста) и количество случаев инсульта у работоспособных пациентов в общей структуре инсультов неуклонно растет. При этом ежегодно от инсульта умирает 45-50 тыс. украинцев, что по показателям смертности от этого заболевания в 2 раза больше, чем в Европе. Инсульты вышли на первое место среди причин смертности в Украине. Потенциальное количество пациентов с нарушением липидного обмена в Украине Составляет более 15 млн. человек, однако обследуются не более 1-2 миллионов. Дислипопротеинемии проявляются изменениями основных липидных параметров в крови. Наиболее частые из них: гиперхолестеринеия, гипертриглицеридемия, высокая концентрация ХС-ЛЛПН, пониженное содержание ХС-ЛПВП.

This book fills an obvious gap in the Handbook series and indeed a major lacuna in the medical literature. Too often investigations of a particular condition are lost in the welter of other text. Alternatively, they appear as specialist books in pathology and radiology. One unique feature of this book is the inclusion of all clinical investigative techniques, i.e. both truly clinical tests in the shape of symptoms and signs and then laboratorybased investigations. This stops what is often an artificial separation. Each section is clearly put together with the intent of easing rapid reference. This is essential if the book is to have (and I believe it does have) real usefulness for bedside medicine. There are many other useful aspects of the text. These include a comprehensive list of abbreviations-the bugbear of medicine, as well as reference ranges which some laboratories still do not append to results. Overall, the Handbook should be of benefit to not just clinical students and junior doctors in training, but all who have patient contact. With this in one pocket, and Longmore in the other, there should be little excuse for errors in diagnosis and investigation, with the added benefit that the balance between the two will allow the upright posture to be maintained.