Current Journal of Applied Science and Technology
39(6): 1-10, 2020; Article no.CJAST.55671
ISSN: 2457-1024
(Past name: British Journal of Applied Science & Technology, Past ISSN: 2231-0843,
NLM ID: 101664541)
Quality Evaluation of Mango Stored in Evaporative
Coolers
A. A. Balogun1*, C. C. Ariahu2 and J. S. Alakali3
1
Institute of Food Security, University of Agriculture, Makurdi, Benue State, Nigeria.
College of Food Technology and Human Ecology, University of Agriculture, Makurdi, Benue State,
Nigeria.
3
Centre for Food Technology and Research, Benue State University, Makurdi, Benue State, Nigeria.
2
Authors’ contributions
This work was carried out in collaboration among all authors. Author AAB designed the study,
performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Author
CCA managed the analyses of the study. Author JSA managed the literature searches. All authors
read and approved the final manuscript.
Article Information
DOI: 10.9734/CJAST/2020/v39i630554
Editor(s):
(1) Dr. Teresa De Pilli, University of Foggia, Italy.
Reviewers:
(1) Ghulam Khaliq, Lasbela University of Agriculture, Water & Marine Sciences (LUAWMS), Pakistan.
(2) Vandré Barbosa Brião, University of Passo Fundo, Brazil.
Complete Peer review History: http://www.sdiarticle4.com/review-history/55671
Original Research Article
Received 27 January 2020
Accepted 02 April 2020
Published 11 April 2020
ABSTRACT
Postharvest loss of fruit and vegetables especially mango, is a major challenge of agriculture. A
research was therefore conducted to evaluate the quality of fresh mango fruits stored in two
evaporative coolers, a non-cladded burnt-clay-brick (NBBEC) and an aluminum-cladded burnt-claybrick evaporative coolers (ABBEC) to reduce postharvest loss. The physicochemical,
microbiological and sensory attributes of mango stored in the coolers and in ambient were
evaluated. Metabolic rates of mango were highest in ambient storage, intermediate in NBBEC with
least values obtained in ABBEC. Beta carotene, ascorbic acid and acidity decreased while total
soluble solids, pH and microbial load increased during storage. Mango stored in aluminum-cladded
burnt-clay-brick evaporative cooler exhibited lower biochemical and physiological reaction rates
hence tissue breakdown, colour changes, pH and titratable acidity were lower in ABBEC than in
NBBEC and ambient storage conditions. ABBEC is therefore recommended for stop gap extension
of shelf life of mango.
_____________________________________________________________________________________________________
*Corresponding author: E-mail:
[email protected];
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
Keywords: Evaporative coolers; physicochemical; microbiological; sensory; mango.
1. INTRODUCTION
reducing the temperature and increasing the
relative humidity in an enclosure, which has been
extensively tried for increasing the shelf life of
horticultural produce in some tropical and
subtropical countries [8]. According to Munoz et
al. [7], ambient temperature and relative humidity
are the main parameters to be considered in
proper storage and preservation of fruits and
vegetables. Towards this end, they developed an
automated electronic evaporative cooler which
increased the shelf lives and quality of
horticultural produce stored in it. Similarly,
Kamaldeen et al. [9] developed a pot-in-pot and
tin-in-pot evaporative coolers wherein mango
was stored. The results showed that tin-in-pot
was better than the pot-in-pot for mango storage
as it retained freshness. Dirpan et al. [10] also
conducted an experiment on the evaporative
cooling of mango. The results showed that
mango stored inside the zero energy cool
chamber (ZECC) were acceptable in quality and
sensory evaluation after eleven days of storage.
The ZECC was able to maintain the quality and
extend the shelf life of mango compared to
ambient storage. The objective of this study
therefore was to evaluate the physicochemical,
microbial and sensory parameters of mango
stored in evaporative coolers in comparison with
those in ambient storage.
Mango (Mangifera indica) belongs to the
Anacardiaceae family. It is an important fruit
grown in many tropical and subtropical regions of
the world. Global production of mango is
concentrated mainly in Asia with India being the
highest producer (11.5 m MT) while Nigeria
produced only about 0.73 m MT [1]. According to
Bhushan [2], India is the major mango producing
country, contributing 40.48 percent of world’s
production while Nigeria occupied the 10th
position in the world ranking of mango producing
countries with 2.13 percent of the world’s
production.
According to Bhushan [2], nutritionally, mango
contains substantial quantity of appreciable beta
carotene, vitamin C and dietary fibre as well as
soluble sugars and different minerals which are
good sources of nutrition readily available and
easily assumable in human body. Islam et al. [3]
observed that both vitamins A and C are
important antioxidants with vitamin C promoting
healthy immune function while vitamin A is
important for vision and bone growth. The dietary
fibre is associated with a reduced risk of some
types of cancer, protecting against heart disease
and cholesterol build-up.
2. MATERIALS AND METHODS
Although Benue State has contributed greatly in
the production of mango which has earned
th
Nigeria its 10 position in the chart of mango
producers in the world, there has not been
significant economic benefit resulting from this
production. This is because a greater part of the
mango produced is lost to poor postharvest
management and lack of good preservation
techniques [4].
2.1 Study Area/Scope of Research
Makurdi is the capital of Benue State, Nigeria.
The town is dominated by guinea savannah type
of vegetation. The mean annual rainfall is
favourable for food production. Makurdi has a
sub-humid, semi-arid tropical climate with mean
annual precipitation at 1200-1300 mm. About
90% of total annual rainfall occurs in the months
of June to September [11]. Temperature rarely
falls below 22°C with peaks of 40 and 30°C in
February/March. In the wet season, the average
temperature is within the range of 23.0-32.7°C.
Data generated were the average for 2014 to
2017 for the evaporative coolers located beside
the College of Food Technology Complex at the
University of Agriculture, Makurdi (Latitude:
07.78915°N, Longitude: 008.61864°E).
While refrigerated cold stores are the best
methods of preserving some fruits and
vegetables, they are expensive to buy and run by
poor resource farmers. According to Nair and
Singh [5], mango fruits are also susceptible to
chilling injury when stored below 13°C.
Consequently, in developing countries, there is
an interest in simple, low-cost alternatives many
of which depend on evaporative cooling which
does not require external power supply [6].
2.2 Design
and
Construction
Evaporative Coolers
Evaporative cooling is an adiabatic cooling
process whereby the air takes moisture which is
cooled while passing through a wet pad or
across a wet surface [7]. Evaporative cooling has
been found to be efficient and economical in
of
Two almost identical burnt-clay-brick evaporative
coolers were designed and constructed adjacent
2
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
and about 1 m apart under two trees. One had
two internal aluminum claddings and was
designated as aluminum cladded burnt-clay-brick
evaporative cooler (ABBEC); the outer aluminum
wall was perforated. The other cooler had no
internal aluminum cladding and was referred to
as non-cladded burnt-clay-brick evaporative
cooler (NBBEC). The pictorial views of the
cooling structures are shown in Plate 1.
Essentially, the evaporative coolers consist of
double jacketed rectangular burnt-clay-brick wall.
The cavity between the inner and outer walls of
each cooler was filled with river-bed sand. The
floors were cemented with mortar (cement, sand
and water mixture) to an even 2 cm thickness.
The doors to the storage spaces were made of
white wood with zinc roofing sheet cladding for
protection against rodents and termites. A makeshift thatched roof cover was built above each of
the coolers to provide extra protection against
direct sunlight in addition to the shade provided
by the trees so that the fullest advantage of
evaporative cooling could be harnessed. In order
to maintain the sand completely wet during the
study, 500 litres of water was used to wet the
sand twice a day [12].
storage. Weight loss for each sample of known
initial weight was calculated as follows:
(%) = (
)/
(1)
100
Where, PWL= product weight loss; W o= initial
weight of sample and W t= weight of sample at
time, t. The mean for the ten samples were then
reported.
2.3.2 Chemical analyses
Chemical analyses were performed according to
the standard official methods described in [13].
Clear juice of mango fruit was extracted by
pulping 100 g of edible portion in a household
electric blender followed by straining using
double-layered muslin cloth.
2.3.3 Ascorbic acid and total carotenoids
Ascorbic acid and carotenoids were determined
by AOAC [13] methods. Ascorbic acid content
was determined on each 10 mL of juice extract
(previously adjusted to pH 1.2 with 1.0M
metaphosphoric acid solution by titration with
0.1% 2,6-dichlorophenol indophenol dye solution.
The ascorbic acid equivalent of the dye was
estimated as follows:
Ascorbic
ℎ
E C1
−
acid
(%)=
mL
dye
×
100
/
(2)
Total carotenoids were determined by mixing 2 g
extract with 20 mL ethanol and 2 mL n-hexane
and 30 mL diethyl ether in a 150 mL separatory
glass funnel. The mixture was shaken vigorously
about 10 times and then allowed to settle for 1
hr. Then, 5 mL each of the upper organic layer
was carefully transferred into clean and labelled
test tubes. The absorbance of each organic
extractive was read at 450 nm wavelength using
1 cm
cuvette of
an ultraviolet/visible
spectrophotometer (Model Jenway 7305).
E C2
Plate 1. Evaporative coolers 1 & 2
EC1= Non-Cladded Burnt-Clay-Brick Evaporative
Cooler (NBBEC); EC2=Aluminum-Cladded Burnt-ClayBrick Evaporative Cooler (ABBEC)
2.3 Commodity Storage Test
2.3.4 Total soluble solids (TSS)
10 kg of ripe mango fruits (Julie vf) were
purchased from Makurdi Wurukum market and
transported to the laboratory in jute bags. They
were then washed with tap water to remove
adhering sand and other foreign matter.
TSS in degree brix was directly measured using
Abbe refractometer (Model: Bellingham &
Stanley Limited, England) by placing a drop of
supernatant on the prism of refractometer.
2.3.5 pH and titratable acidity determination
2.3.1 Weight loss
The digital pH meter (Model pH 211, HI Hanna
Instruments, Italy) was used to measure the pH
of the mango juice while total titratable acidity
(expressed as citric acid %) was determined by
titrating 5 ml of mango juice with 0.1N sodium
Weight loss was measured before and after
storage using an electronic weighing balance
(Model: Mettler P1210). Ten mango fruits were
drawn at random on the 1st, 5th and 10th days of
3
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
storage on the tenth day of storage (Fig. 1).
According to Ubwa et al. [4], water loss through
lenticel seems to be the possible reason for
physiological weight loss in mango during
storage. Esguerra and Rolle [17] explained that
when harvested, mango fruit can no longer
replace the water that is lost through respiration,
it is therefore subjected to shriveling and weight
loss, and consequently loss in marketable weight
resulting in deteriorative appearance. According
to [18], weight loss is primarily associated with
the fruit respiration and evaporation of moisture.
The percentage weight loss increased with
increase in storage period [19]. The increase in
weight loss with storage period may be due to
reduction in moisture content on respiration [10].
hydroxide-using phenolphthalein as an indicator
[13].
2.4 Microbiological Analysis
Samples for total plate counts and fungal counts
were prepared as described by Harrigan and
McCance [14]. Triplicate 2 g portions of mango
fruit were sliced and homogenized in a Warring
blender which was previously washed and
sterilized with 100 ppm sodium hypochlorite
solution and rinsed with sterile deionized water.
Serial dilutions of homogenate ranging from 10-1
-5
to 10
were obtained using sterile saline
solution. Total aerobic plate counts and fungal
counts were performed on nutrient agar and
Saboraud dextrose agar respectively using the
pour-plate method described by Harrigan and
McCance [14].
3.2 Chemical Analysis
2.5 Sensory Evaluation
3.2.1 Ascorbic acid and total carotenoids
A consistent panel of 12 semi-trained judges was
used to evaluate the appearance, texture and
overall acceptability of mango sample using the
descriptive sensory profile developed based on
perceptions of the judges for quality of fruits and
vegetables. Sensory evaluation was conducted
under fluorescent light in a special sensory
testing room with partitioned booths. The
degrees of preference based on the descriptive
terms were then converted to scores with 7=very
firm and 1=Putrid/mushy for texture, 7=very fresh
and 1=extremely mouldy for appearance and
7=highly acceptable and 1=disgusting for overall
acceptability [15].
3.1 Physiological Loss in Weight
Fig. 2 shows the effect of storage condition on
the ascorbic acid content of mango. The ascorbic
acid content of fresh mango fruits before storage
was 22.09 mg/100 g which decreased
significantly (p<0.05) to 15.52 mg/100 g in
ambient, 17.15 mg/100 g in NBBEC and 19.38
mg/100 g in ABBEC storage. There was no
significant difference (p<0.05) between mango
th
st
stored in ABBEC on the 7 and 21 days of
storage. However, mango fruits stored at NBBEC
and ambient recorded significant differences
th
st
(p>0.05) on day 7 and 21 . Similarly, the
ascorbic acid content of mango fruits stored in
ABBEC were significantly higher than those in
NBBEC and ambient storage conditions. This
could be due to the relatively lower temperature
and higher relative humidity which retards
senescence through reduced respiration rate and
other undesirable metabolic changes [10].
Bhushan [2] and Hossain et al. [20] reported
slightly lower values of 16 mg/100 g and within
the range of 2.1 to 10.4 mg/100 g respectively.
The retention of ascorbic acid has been used as
an estimate of the overall nutrient retention in
food product as it is the most unstable nutrient
[21]. Previous studies Morris et al. [22] and Lee
and Nagy [23] have reported that storage
duration and condition are important parameters
in ascorbic acid degradation.
In this study, significant differences (P>0.05)
were found in mango fruits stored in the
evaporative coolers (NBBEC and ABBEC) and at
ambient storage conditions. Mango had the
highest weight loss at ambient (25%), 13.4% in
NBBEC and the least value of 6.7% in ABBEC
The effect of storage conditions on the beta
carotene content of mango is shown in Fig. 3.
There were significant differences between the
beta carotene values of mango at the different
storage conditions. The beta carotene content
ranged between 1013.1 to 2119 µg/100 g in this
2.6 Statistical Analysis
The results obtained were evaluated using the
analysis of variance with the aid of Statisca 6.0
software package (Stafso, Inc. USA). The means
of factors showing significant (p=0.5) differences
were separated using Tukey’s LSD test [16]. For
this storage studies with mango, the variables
evaluated were influences of 3 storage times (0,
th
th
5 and 10 days) and 3 storage conditions
(Atmosphere, NBBEC and ABBEC).
3. RESULTS AND DISCUSSION
4
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
study. Badifu et al. [24] reported slightly higher
value of 2400 µg/100 g for mango in their study.
According to Bolanle et al. [25], differences in
beta carotene may be a reflection of differences
in species/cultivars which is genetically
determined.
degradation of polysaccharides to simple sugars.
The total soluble solids of mango (10.56 – 14.51
o
Brix) were significantly (p<0.05) different at all
the storage conditions. The results indicated that
ABBEC stored mango fruits increased to
o
12.00 Brix while ambient stored mangoes
o
increased to the highest value of 14.51 Brix after
ten days of storage. Akhter et al. [27] reported
similar values which ranged between 5.1 to
12.9%. Naz et al. [28] reported slightly
higher values that ranged between 11.60 to
19.83%. According to Okoth et al. [29],
3.2.2 Total soluble solids
Total soluble solid is a measure of the degree of
ripeness in fruits. According to [26], increase in
total soluble solids during ripening is due to the
PWL
10 days
0.3
0.25
0.2
0.15
0.1
0.05
0
Ambient
NBBEC
ABBEC
Fig. 1. Effect of storage conditions on physiological loss in weight of mango
25
20
15
10
5
0
Storage time
Ambient
Series1
NBBEC
Series2
ABBEC
Series3
Fig. 2. Effect of storage conditions on the ascorbic acid content of mango
5
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
2500
2000
1500
1000
500
0
Storage time
Ambient
Series1
NBBEC
Series2
ABBEC
Series3
Fig. 3. Effect of storage conditions on the beta carotene of mango
TSS has a strong implication on the choice of
fruit for processing as well as fresh consumption.
The authors suggested that the variability in TSS
of mango at different stages of maturity is
attributed to the alteration occurring in structure
during ripening processes at various hydrolytic
processes resulting in the breakdown of complex
carbohydrates to smaller ones like sucrose,
glucose and fructose.
were between 0.36 to 0.87%, while the result of
[28] were slightly lower (0.12 - 0.49%). The
decrease recorded in this study might be due to
the inhibited activities of enzymes to change the
titratable acidity contents due to the reduced
temperature and high RH of the evaporative
coolers.
3.2.3 pH and total titratable acidity
The effect of storage conditions on the microbial
load of mango as presented in Table 2 indicated
that the total plate count ranged between 1.40 to
3.32 Log10 CFU/g while yeast and mould count
ranged between 1.05 and 2.44 Log10 CFU/g. In
this study, the microbial load on mango fruit was
lowest on ABBEC stored fruits and highest on
ambient stored fruits. Similarly, there were no
significant difference between the mango fruits at
the initial storage and day 7 in ABBEC storage
conditions. This could be due to the lower
temperature and high relative humidity of
ABBEC. Also, the aluminum incorporated in
ABBEC storage further improved the microbial
load due to its lower heat sink and being a good
thermal and electrical conductor. These values
were much lower than the bacterial counts of
4.90 to 5.90 Log10 CFU/g and fungal counts of
4.0 Log10 cfu/g reported by Ogbogu et al. [31].
Akinmusire [32] observed that numerous
microbial defects of fruits and vegetables are
characterized by the types of microorganisms
responsible for the deterioration. Spoilt mango is
characterized by tissue softening, formation of rot
and mycelia, moisture loss, unpleasant odour
and shrinkage.
3.3 Microbiological Analysis of Mango
A slight significant increase in pH (p<0.05) was
observed during storage of mango (Table 1).
Higher increase in pH was observed at ambient
higher temperatures. The mango had initial pH
value of 3.64 before storage which increased to
4.70 in ambient, 4.07 in NBBEC and 3.94 in
ABBEC storage conditions. Metabolic activities of
the fruit could lead to changes in pH. This result
compared closely with the findings of Ilesanmi et
al. [30] who reported pH values between 4.02
and 5.47. pH in the fruit pulp plays an important
role in flavour promotion as well as a
preservation factor.
A decrease in titratable acidity was observed in
mango at the three storage conditions. However,
ambient condition with higher temperature
showed higher decline in titratable acidity. The
changes in total titratable acidity were
significantly affected by the rate of metabolism
especially respiration which consumed citric acid.
The titratable acidity result (0.78 - 0.90%) for
mango obtained in this study had a similar trend
with that of Ilesanmi et al. [30] whose values
6
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
Table 1. Effect of storage conditions on TTA, TSS and pH of Mango fruit
Parameter
TSS (oBrix)
Storage time (Days)
0
5
10
0
5
10
0
5
10
TTA (%)
pH
Ambient
10.56c
b
12.35
e
14.51
b
0.90
b
0.81
b
0.78
3.64b
b
3.99
a
4.70
NBBEC
10.56c
d
11.47
b
12.10
b
0.90
b
0.87
b
0.84
3.64b
b
3.97
a
4.07
ABBEC
10.56c
d
11.25
bd
12.00
b
0.90
b
0.88
b
0.83
3.64b
b
3.75
ab
3.94
LSD
0.83
0.21
0.88
Each value is the mean of triplicate determinations for 2014-2017
Values for each parameter with common superscripts are not significantly (p>0.05) different
NBBEC= Non-cladded burnt-clay-brick evaporative cooler,
ABBEC= Aluminum-cladded burnt-clay-brick evaporative cooler
TSS= Total Soluble Solids, TTA= Total Titratable Acidity
LSD = Least Significant Difference
Table 2. Effect of storage conditions on microbial load of mango
Microbial parameter
Total plate count
(Log10 cfu/g)
Yeast & mould count
(Log10 cfu/g)
Storage time
(Days)
0
5
10
0
5
10
Storage condition
ambient
1.40a
a
1.89
c
3.32
1.05b
1.29a
d
2.44
Storage condition
NBBEC
1.40a
ab
1.60
d
2.18
1.05b
1.15b
d
2.32
Storage condition
ABBEC
1.40a
a
1.74
d
2.11
1.05b
1.12b
a
1.24
NBBEC= Non-cladded burnt-clay-brick evaporative cooler
ABBEC= Aluminum-cladded burnt-clay brick evaporative cooler
Values for each parameter with common superscripts are not significantly (p>0.05) different
Table 3. Effect of storage conditions on sensory scores of mango
Sensory attribute Storage time
(Days)
Appearance
0
5
10
Texture
0
5
10
Overall
0
acceptability
5
10
Storage condition
Ambient
6.71a
b
5.18
3.55d
6.40a
c
4.85
3.05d
6.89a
bc
4.96
3.03d
Storage condition
NBBEC
6.71a
b
5.44
4.12c
6.40a
b
5.33
4.13c
6.89a
b
5.61
4.32c
Storage condition
ABBEC
6.71a
b
5.48
4.40c
6.40a
a
6.05
4.57bc
6.89a
b
5.77
4.64c
Values for each attribute with common superscripts are not significantly (p>0.05) different.
Each result is the mean of 12 panelists responses on a scale with 7=excellent and 1=very poor.
ABBEC=Aluminum-cladded burnt-clay-brick evaporative cooler NBBEC= Non-cladded burnt-clay-brick
evaporative cooler
3.4 Sensory Evaluation of Mango
days at ambient while mango fruits in ABBEC
and NBBEC had shelf life of ten days. Table 3
also shows the texture of mango fruits at the
three storage conditions. Fruit firmness showed
significant decrease at the different storage
conditions. ABBEC storage showed better
retention of fruit firmness. Abdalla et al. [33]
The effect of storage condition on sensory scores
of mango in this study is presented in Table 3.
The change in colour was more pronounced on
mango kept in ambient. Spoilage of mango due
to end rot limited its storage potential to only four
7
Balogun et al.; CJAST, 39(6): 1-10, 2020; Article no.CJAST.55671
COMPETING INTERESTS
reported that mango stored in ambient showed
deterioration in fruit firmness while those stored
in evaporative coolers showed better retention of
firmness. Mango stored at ambient developed
black spots, lost their firmness and softened.
According to Narayana et al. [18] when mango
fruit loses weight, shriveling occurs and the
appearance deteriorates thus reducing its market
value. In agreement with the present study, the
authors observed that mango being a climacteric
fruit possesses a very short shelf life and reach
respiration peak of ripening process on the third
or fourth day of storage at ambient temperature.
Authors have
interests exist.
declared
that
no
competing
REFERENCES
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The loss of firmness in ambient storage was due
to cell wall digestion by pectin esterase,
polygalacturonase and other enzymes, and this
process increased with increase in storage
temperature [10]. According to Narayana et al.
[18] and Shahnawaz et al. [34], the loss of water
from mango fruit due to respiration and
transpiration results in loss of weight, shriveling
and deteriorative appearance. On overall
acceptability, panelists had higher scores for
ABBEC stored mango fruits which ranged from
4.64 to 6.89. Overall acceptability of mango in
this study is in agreement with the findings of [35]
that lowering temperature reduces respiration
while high storage temperature hastens
senescence of fruits. Generally, mango stored in
ABBEC were superior to mango in ambient
storage at the end of the ten-day storage period.
The ABBEC stored mango exhibited slower
decay and lower water loss as a result of lower
temperature and higher relative humidity of the
evaporative cooler.
2.
3.
4.
5.
4. CONCLUSION
6.
The use of evaporative coolers for the storage of
mango fruits or any other agricultural
commodities would maintain their freshness and
increase storage life better than in ambient
condition due to the lower temperature and
higher relative humidity exhibited by the coolers.
Mango fruits stored in evaporative coolers
showed slower decay and lower water loss than
ambient stored fruits. Due to the lower
temperature and higher relative humidity of the
evaporative coolers during storage, mango
quality was better maintained than in ambient
storage. ABBEC storage was found to be the
most efficient method due to its effects on
reducing respiration rate, transpiration, ethylene
production and senescence. ABBEC storage is
therefore recommended as a stop gap extension
of shelf life of mango fruits.
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