Posters by John A Lucci
After the dengue virus binds to the myeloid progenitor cell receptor, it undergoes conformational... more After the dengue virus binds to the myeloid progenitor cell receptor, it undergoes conformational rearrangement in response to the reduction of pH. Flaviviruses activate fusogens to induce membrane fusion yielding syncytia, but Dengue is unable to form sufficient fusion pores. A 3D structural analysis shows a shift in the envelope ectodomain (sE) from a dimeric extracellular form to a trimeric intracellular form. The trimer interacts with the endosome membrane using elongated fusion loops. to prevent membrane fusion, relevant envelope antigen binding sites were analyzed through blastP for homology, which was found between Zika Virus and Dengue 4 Virus. This presents an opportunity for formulation of a degenerate inter-epitope vaccine capable of inducing the persistence of cross-reactive neutralizing antibodies for Binding of the trimeric spike to prevent the transient fusogenic trimers of E glycoproteins from initiating viral fusion and nucleocapsid penetration into the cytoplasm.
The age of active site analogues has passed; modern biochemistry has provided an extensive librar... more The age of active site analogues has passed; modern biochemistry has provided an extensive library, but it is the molecular biology of mother nature that has infinite methods of genetic engineering. Transfection is transiently episomal. Viral transduction occurs at specific sequences in certain cells as determined by capsid-cell surface receptor interaction determined tissue tropism. To counteract inefficiency, capsid variants have been observed across serotypes for tissue targeting tropism to a primary glycan receptor. Capsid engineering has led to higher transfection rates due to further selection of tissue specific receptor binding
sites by residue variations in several capsid peptides. Analysis was partaken using VMD and PyMOLof PDB files from RCSB displaying 8 capsid states Dependoparvovirus
Presented at the Academy of Integrative Health & Medicine 2016 Annual Conference: People, Planet ... more Presented at the Academy of Integrative Health & Medicine 2016 Annual Conference: People, Planet Purpose Global Practitioners United in Health and Healing.
Reduction of Erythematous Autoimmune Symptoms due to Curcumin inhibition of NFATc Activation
Presentations by John A Lucci
Morphology, IHC, FISH, & off label Venetoclax treatment
Presentation on High Spatial Resolution in 4D Light Sheet Fluorescence Microscopy, in which the i... more Presentation on High Spatial Resolution in 4D Light Sheet Fluorescence Microscopy, in which the illumination laser path is positioned perpendicularly to the detection sample. The illumination laser beam is shaped into a rectangle and then focused orthogonally into a thin sheet of light. As the sample moves through the focal plane, different planes of the sample are illuminated, creating a z-stack of images that can be compiled and three-dimensionally reconstructed
Presentation stating why the adeno-associated viruses prove to be an ideal vectors as a result of... more Presentation stating why the adeno-associated viruses prove to be an ideal vectors as a result of their abilities as
non-pathogenic,
non-oncogenic, and
ignorance of immune excitation.
The primary attribute that makes adeno-associated viral vectors ideal is their ability to infect non-diving cells, instigating repeatable chromosomal changes leading to long-term expression, an attribute which may not be necessary in the hippocampus, but is most definitively necessary for a majority of neurons as a result of their terminal differentiated nature.
In vitro by John A Lucci
Isolation of Soil Microbiotia from North Woods, Kingston, RI and Thermocline microbial communitie... more Isolation of Soil Microbiotia from North Woods, Kingston, RI and Thermocline microbial communities of Watchaug Pond, Charleston, RI
Quantification using the dsDNA High Sensitivity Assay was partaken using the Qubit Fluorometer sh... more Quantification using the dsDNA High Sensitivity Assay was partaken using the Qubit Fluorometer showing 5.4 ug/mL. After gel extraction, Nested PCR reaction was performed with degenerate nifH primers. The PCR product was ligated into a Carbenicillin-resistant pGem-T vector which was used to transform JM109 Z cells using CaCl2 Sucessful transformation was indicated by the insertion of the pGEM vector into the lacZ gene, which when the cells were grown on Carbenicillan plates containing X-gal, (galactosidase and indole), a colorless modified galactose sugar that when metabolized (cleaved) by β-galactosidases, forms an insoluble product which is bright blue. The insertion of the vector was confirmed by growing the cells on carbenicillin plates, which only allows cells with the vector to grow, because vector has a resistance gene.
cells. Quantification of plaque formation was attempted during the lytic phase of Bacteriophage P... more cells. Quantification of plaque formation was attempted during the lytic phase of Bacteriophage P1 when incubated with E. coli. The phage particle injects its single-stranded DNA into the bacterium using its tail structure like a syringe, which contracts driving a tube through the pore into the cytoplasmic membrane of the bacterium. Once internalized the phage DNA blocks normal bacterial synthesis and metabolism and begins to code for the production of new phage particles, using the cellular machinery of the host. The spread of infectious phage from the initially infected bacterial cells to the surrounding cells results in lysis of the bacteria in the vicinity of the initial phage particle and hence the zone of cl earing. The soft agar used in these assays permits diffusion of the phage to nearby uninfected cells but the phage does not continue to spread because heavy inoculation induces inhibition of lysis. The number of plaques that develop into the determined dilution factors can be used to calculate the number of bacteriophages in a sample.
Pathogenesis is a multifaceted concept, dependent on a variety of factors, but as Dr. Paul S. Coh... more Pathogenesis is a multifaceted concept, dependent on a variety of factors, but as Dr. Paul S. Cohen recently entailed in the recently published Metabolism and Bacterial Pathogenesis, if an organism cannot make use of the nutrients available in its environment, it cannot reproduce efficiently enough to maintain survival against competitive species. Salmonella enterica serovariant Typhimurium is able to utilize phosphatidylserine, an abundant component of eucaryotic cell membranes, promoting its pathogenesis. Cohen, et al. isolated several mutants unable to utilize oleate as a sole carbon source using transposon mutagenesis and induced LJ2443, a cra mutant unable to grow on minimal agar plates containing gluconeogenic substrates as sole carbon sources. These properties were similar to SR-11 Fad- which was found to be five orders of magnitude less pathogenic in mouse liver and spleen than wildtype SR-11. Eventually, the wild-type SR-11 cra gene was cloned into pBR322 to create pJHA8, a 1.4-kb plasmid containing the cra+ gene in PstI site of pBR322 and the tetR resistance marker.1 Transformation of plasmid pJHA8 into S. typhimurium SR-11 LJ2443 cells was partaken using the electroporation method. Electroporation method was employed to create an electric field which induced a voltage across the cell membrane, introducing the plasmid into the Salmonella cells. Plasmid pJHA8, which contains operons cra+ and tetR, was then spread on plates containing tetracycline to select for proper transformation due to the tetR selective marker on the plasmid encoding for tetracycline resistance. Colonies from these plates were then isolated and deposited onto the slants comprised of the defined enrichment medium Simmons’ citrate, which as well as containing citrate as a sole gluconeogenic substrate contain bromothymol blue, a pH indicator which is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.
Objective: To analyze phenotypic variations of galactoside cleavage caused by lac repressor (lac... more Objective: To analyze phenotypic variations of galactoside cleavage caused by lac repressor (lacI) induction by IPTG (Isopropyl β-D-1-thiogalactopyranoside), β-galactosidase (lacZ) cleavage of ONPG (O-nitrophenyl-β-D-galactopyranoside) into O-nitrophenol, and β-galactoside permease (lacY) membrane permeability in E. coli strains ML35 (lacI- lacZC lacY-) and K12 (lacI+ lacZ+ lacY+). The absorbance of the product of ONPG cleavage is representative of activity when reflected against o-nitrophenol absorbance across chemical disruptors of gene product, such as lacY effector toluene and and lacl effector IPTG.
Mutations, even at the most miniscule single-point occurrence, have be non-conservative missense ... more Mutations, even at the most miniscule single-point occurrence, have be non-conservative missense mutations with the ability to change the primary amino acid sequence the final protein product and thus can effect efficiency of enzymes and the overall viability of the cell. A selective mutation imparts a survival advantage to the cell, while a non-selective mutation imparts no such benefit. By adjusting culture conditions, cells with selective mutations can be induced in a more rapid rate than in environmental conditions and selective mutations can also easily be detected by comparison of growth in selective and non-selective culture. In this experiment the spontaneous Streptomycin-resistant mutant forms of E. coli are isolated. When exposed to a growth medium containing inhibitory levels of antibiotics, any cells that are able to form colonies have acquired resistance due to prior mutation. E. coli, a strain sensitive to 0.01 mg/mL streptomycin, is used to quantify the frequency of occurrence of resistant mutants.
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Posters by John A Lucci
sites by residue variations in several capsid peptides. Analysis was partaken using VMD and PyMOLof PDB files from RCSB displaying 8 capsid states Dependoparvovirus
Reduction of Erythematous Autoimmune Symptoms due to Curcumin inhibition of NFATc Activation
Presentations by John A Lucci
non-pathogenic,
non-oncogenic, and
ignorance of immune excitation.
The primary attribute that makes adeno-associated viral vectors ideal is their ability to infect non-diving cells, instigating repeatable chromosomal changes leading to long-term expression, an attribute which may not be necessary in the hippocampus, but is most definitively necessary for a majority of neurons as a result of their terminal differentiated nature.
In vitro by John A Lucci
sites by residue variations in several capsid peptides. Analysis was partaken using VMD and PyMOLof PDB files from RCSB displaying 8 capsid states Dependoparvovirus
Reduction of Erythematous Autoimmune Symptoms due to Curcumin inhibition of NFATc Activation
non-pathogenic,
non-oncogenic, and
ignorance of immune excitation.
The primary attribute that makes adeno-associated viral vectors ideal is their ability to infect non-diving cells, instigating repeatable chromosomal changes leading to long-term expression, an attribute which may not be necessary in the hippocampus, but is most definitively necessary for a majority of neurons as a result of their terminal differentiated nature.
(ethylene diamine tetra acetic acid), a metal ion chelator that acts to remove Ca2+ and Mg2+ from the outer membrane, causes holes appear in the membrane due to electrostatic repulsion. After the addition of EDTA, the lysozyme can reach the cells. The outer membrane of the gram (+) B. pumilus is a physical barrier so that the lysozyme cannot contact the peptidoglycan. The enzyme functions as a muramidase by attacking peptidoglycans and hydrolyzing the glycosidic bond that connects N-acetylmuramic acid with the fourth carbon atom of N-acetylglucosamine.
Proliferation Indicies, Clusters of Differentiation,
and Fluorescence In-situ Hybridization