Papers by Aris Miron
Journal of Food Processing and Technology, Aug 24, 2015
The medicinal mushroom Ganoderma lucidum (Ling Zhi or Reishi) is a basidiomycete white rot macrof... more The medicinal mushroom Ganoderma lucidum (Ling Zhi or Reishi) is a basidiomycete white rot macrofungus which has been recognized as a medicinal mushroom for over 2000 years. Ganoderma lucidum is a source of polysaccharides with immunostimulatory, anticancer, hypocholesterolemic, hypoglycemic and other bioactive properties and is used in the treatment of several diseases. The primary objective of this study was to investigate the effect of different bioprocess conditions (pH, agitation, medium composition etc) on the production of G. lucidum biomass, extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) in submerged cultures. Also, mycelium morphology was analyzed through image analysis software and parameters such as pellet/hyphae formation, density, perimeter, roundness, length and width were studied in order to clarify how they may influence biomass, EPS and IPS accumulation in submerged cultures. A low agitation rate (e.g., 150 rpm) was preferable for biomass production and polysaccharide synthesis while the addition of organic nitrogen (peptone) increased biomass and IPS concentration in comparison to the use of ammonium salts. In addition, a medium with pH 5 was the most favorable for IPS and EPS production. Changes in mycelium morphology and EPS, IPS production were observed when Ganoderma lucidum was grown at different pH conditions (4.5, 5.0, 5.5 and 6.0). Based on the morphological observations we concluded that increased density values from 2th to 4th and 6th days improved IPS production while a significant reduction in density values was correlated with high EPS production which was observed from the 9th to the 18th day of fermentation.
Journal of Food Processing and Technology, Aug 24, 2015
Journal of Food Processing and Technology, Aug 24, 2015
In recent years, a couple of bioreactor modifications have been developed and successfully adopte... more In recent years, a couple of bioreactor modifications have been developed and successfully adopted for the in vitro cultivation of plant cells and microorganisms. Bioreactors such as mechanically agitated, airlift and photobioreactors have been designed and used for large scale cultivation of algal, higher biomass and bioactive compounds production.
Our primary objective of this study was to build and design a cost effective and ecofriendly airlift bioreactor 4L in which the agitation rate (i.e. 150, 250, 500 rpm) will be controlled through computer software without affecting the personal computer performance (temperature, graphics).
As a result we've designed and develop the first computer in the world that is be able to do more than a simple computer can do, and that is fermentation. This project can give rise to important achievements that every person in the world who has access to a desktop computer, is be able to do. Furthermore, due to its ecofriendly attitude, is that our computer tower has designed with Plexiglas that can be broken down into its original chemical constituents or directly and completely recycled.
If you plan to attend 29th EFFoST, we would appreciate the opportunity to meet you.
Ganoderma lucidum is a source of polysaccharides with immunostimulatory, anticancer, hypocholeste... more Ganoderma lucidum is a source of polysaccharides with immunostimulatory, anticancer, hypocholesterolemic, hypoglycemic and other bioactive properties and is used in the treatment of several diseases. The primary objective of this study was to investigate the effect of different bioprocess conditions (pH, agitation, medium composition, etc) on the production of G. lucidum biomass, extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) in submerged cultures. Also, mycelium morphology was analyzed through image analysis software and parameters such as pellet/hyphae formation, density, perimeter, roundness, length and width were studied in order to clarify how they may influence biomass, EPS and IPS accumulation in submerged cultures.
A comprehensive public health approach is needed to achieve a significant reduction in sodium int... more A comprehensive public health approach is needed to achieve a significant reduction in sodium intake. A successful approach involves various strategies, which will jointly promote positive decisions. The strategies include public education, individual dietary advice, food labeling and coordinated voluntary reduction of sodium in industry.
Conference Presentations by Aris Miron
Ganoderma lucidum is probably the most significant pharmaceutical (anticancer, immunostimulating,... more Ganoderma lucidum is probably the most significant pharmaceutical (anticancer, immunostimulating, hypoglycemic, hypocholesterolemic, etc) mushroom the biological properties of which are due to the β-glucans and other polysaccharides that it either excretes (exopolysaccharides) or accumulates in the cytoplasm (endopolysaccharides). The submerged culture of Ganoderma lucidum is an alternative to the production on solid substrates which offers mush faster growth and more easily controlled and optimized culture conditions. Here, several strategies for improving biomass, exopolysaccharide and endopolysaccharide production are discussed, namely the effect of sugar concentration, the type of nitrogen sources, the agitation rate, the process pH, the effect of light/darkness, the effect of additional nutrients (glycerol, vegetable oils) which play a significant role in submerged cultures. For instance, it was observed that peptone was optimal nitrogen source for better cell growth, although exopolysaccharide production improved with use of inorganic nitrogen sources. Also a low agitation rate and a pH at 5 were preferable for both biomass and polysaccharide production. The use of soya oil could increase biomass and endopolysaccharide production, but not exopolysaccharide synthesis, while glycerol was beneficial for exopolysaccharide synthesis, but not for cell growth. The effects of different process parameters on fungal morphology in submerged cultures is also discussed and the influence of pellet formation versus hyphae formation upon polysaccharide production is described. Lastly, different formulation of solid substrates for G. lucidum production are tested and compared using potential growth-stimulating factors (organic and inorganic nitrogen sources, vegetable oil,
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Papers by Aris Miron
Our primary objective of this study was to build and design a cost effective and ecofriendly airlift bioreactor 4L in which the agitation rate (i.e. 150, 250, 500 rpm) will be controlled through computer software without affecting the personal computer performance (temperature, graphics).
As a result we've designed and develop the first computer in the world that is be able to do more than a simple computer can do, and that is fermentation. This project can give rise to important achievements that every person in the world who has access to a desktop computer, is be able to do. Furthermore, due to its ecofriendly attitude, is that our computer tower has designed with Plexiglas that can be broken down into its original chemical constituents or directly and completely recycled.
If you plan to attend 29th EFFoST, we would appreciate the opportunity to meet you.
Conference Presentations by Aris Miron
Our primary objective of this study was to build and design a cost effective and ecofriendly airlift bioreactor 4L in which the agitation rate (i.e. 150, 250, 500 rpm) will be controlled through computer software without affecting the personal computer performance (temperature, graphics).
As a result we've designed and develop the first computer in the world that is be able to do more than a simple computer can do, and that is fermentation. This project can give rise to important achievements that every person in the world who has access to a desktop computer, is be able to do. Furthermore, due to its ecofriendly attitude, is that our computer tower has designed with Plexiglas that can be broken down into its original chemical constituents or directly and completely recycled.
If you plan to attend 29th EFFoST, we would appreciate the opportunity to meet you.