Papers by krishnaveni Devulapalli
Background: Gastric Cancer (GC) is one of the most commonly diagnosed malignancies. Genetic varia... more Background: Gastric Cancer (GC) is one of the most commonly diagnosed malignancies. Genetic variation in genes encoding cytokines and their receptors, determine the intensity of the inflammatory response, which may contribute to individual differences in the outcome and severity of the disease. Interleukin-10 (IL-10) is a multifunctional cytokine with both immunosuppressive and antiangiogenic functions. Polymorphisms in the IL-10 gene promoter genetically determine inter-individual differences in IL-10 production. In the present study, we investigated the association between the IL-10 –1082 G/A polymorphism and the susceptibility to gastric cancer in a South Indian population from Andhra Pradesh.
Your article is protected by copyright and all rights are held exclusively by Association of Clin... more Your article is protected by copyright and all rights are held exclusively by Association of Clinical Biochemists of India. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Your article is protected by copyright and all rights are held exclusively by Association of Clin... more Your article is protected by copyright and all rights are held exclusively by Association of Clinical Biochemists of India. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Saudi Journal of Gastroenterology, 2014
Background/Aims: Gastric cancer (GC) is a multifactorial disorder mediated by genetic, epigenetic... more Background/Aims: Gastric cancer (GC) is a multifactorial disorder mediated by genetic, epigenetic, and environmental risk factors. GC is the most common cancer in India and it is the third prominent cause of cancer death worldwide. A single nucleotide polymorphism (SNP) in the promoter region of interstitial collagenase (MMP-1) gene appears to have an impact on the transcriptional activity and regulation of its expression. Hence, the present study is aimed to evaluate the role of interstitial collagenase gene-1607 1G/2G (rs1799750) promoter polymorphism in the etiology of GC. Patients and Methods: The study included 166 GC patients and 202 control subjects. Genomic DNA was isolated from whole blood samples of the subjects, and the genotyping of interstitial collagenase promoter polymorphism was carried out by polymerase chain reaction-restriction fragment length polymorphism method followed by agarose gel electrophoresis. Appropriate statistical methods were applied to test the significance of the results.Results: The risk factor profile of the patients revealed that male gender, age above 50 years, addiction to alcohol and smoking were the most common risk factors (P < 0.05).
Indian Journal of Clinical Biochemistry, 2011
Aim To study the role of 5A/6A polymorphism of matrix metalloproteinase (MMP-3) and their levels ... more Aim To study the role of 5A/6A polymorphism of matrix metalloproteinase (MMP-3) and their levels in the pathogenesis of chronic pancreatitis (CP). Methods One hundred and twenty CP patients and an equal number of age and sex-matched healthy controls were included in the study. Genotypes were determined for 5A/6A allele of MMP-3 gene by allele specific PCR (AS-PCR). The serum MMP-3 levels were estimated using sandwich ELISA method. Results The distribution of the genotypes of the 5A/6A polymorphism in both control and study patients was similar (p=0.523). Within the disease group, patients with older age, early onset of the disease, and addictions such as smoking and alcohol consumption had higher levels as compared to those who did not have these features. Conclusion We conclude that functional polymorphism of MMP-3 (5A/6A) is not associated with CP. However, the higher levels within the disease group indicate its possible role in the disease process.
Indian Journal of Clinical Biochemistry, 2012
Your article is protected by copyright and all rights are held exclusively by Association of Clin... more Your article is protected by copyright and all rights are held exclusively by Association of Clinical Biochemists of India. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Saudi Journal of Gastroenterology, 2013
Indian Journal of Gastroenterology, 2011
Aim To study the role of 5A/6A polymorphism of matrix metalloproteinase (MMP-3) and their levels ... more Aim To study the role of 5A/6A polymorphism of matrix metalloproteinase (MMP-3) and their levels in the pathogenesis of chronic pancreatitis (CP). Methods One hundred and twenty CP patients and an equal number of age and sex-matched healthy controls were included in the study. Genotypes were determined for 5A/6A allele of MMP-3 gene by allele specific PCR (AS-PCR). The serum MMP-3 levels were estimated using sandwich ELISA method. Results The distribution of the genotypes of the 5A/6A polymorphism in both control and study patients was similar (p=0.523). Within the disease group, patients with older age, early onset of the disease, and addictions such as smoking and alcohol consumption had higher levels as compared to those who did not have these features. Conclusion We conclude that functional polymorphism of MMP-3 (5A/6A) is not associated with CP. However, the higher levels within the disease group indicate its possible role in the disease process.
American Journal of Reproductive Immunology, 2011
Gastric cancer is a major cause of cancer death worldwide, especially in developing countries. Th... more Gastric cancer is a major cause of cancer death worldwide, especially in developing countries. The incidence of gastric cancer varies from country to country, probably as a result of genetic, epigenetic, and environmental factors. H. pylori infection is considered as a major risk factor in the development of gastric cancer. However, the scenario varies in Asian countries, exhibiting a higher rate of H. pylori infection and low incidence of gastric cancer, which could be attributed to strain-specific virulence factors and host genetic makeup. In this review, we discuss the various virulence factors expressed by this bacterium and their interaction with the host factors, to influence pathogenesis.
AIM: To investigate the role of endothelial nitric oxide
synthase -786 T > C promoter polymorphis... more AIM: To investigate the role of endothelial nitric oxide
synthase -786 T > C promoter polymorphism in the
etiology of gastric cancer.
METHODS: A total of 150 GC patients and 150 control
subjects were included in the study. The information
on demographic features was elicited with an informed
consent from all the patients and control subjects using
a structured questionnaire. Helicobacter pylori (H. pylori )
infectivity status was tested in antral biopsies from all
the subjects by rapid urease test following the method
of Vaira et al . Genomic DNA was isolated from whole
blood samples following the salting out method of Lahiri
et al . Genotype analysis of the rs2070744 polymorphism
was carried out by allele-specific polymerase chain reaction method. The genotypes were determined
based on the appearance of bands on an agarose gel
stained with ethidium bromide under ultraviolet gel
documentation with the help of 100 bp ladder. Odds
ratios and corresponding 95% confidence intervals were
determined using java stat online software.
RESULTS: There was a significant difference in
the distribution of C allele (C vs T ; P = 0.000, OR
= 5.038) in patient group compared to the control
subjects exhibiting a fivefold increased risk for gastric
cancer. When the T/T and C/C genotypes were
compared, there was an enhanced gastric cancer risk
for individuals with C/C genotype (T/T vs C/C ; P =
0.000). Among the demographic factors, smoking and
alcoholism were the common risk factors in patients
compared to the control subjects (P < 0.05). Patients
with smoking and alcoholism developed cancer even
in heterozygous T/C condition (smoking: P = 0.020
and alcoholism: P = 0.005). Individuals with H. pylori
infection showed seven fold increased risk for cancer.
All the patients with C/C genotype revealed a significant
association between H. pylori infection and gastric
cancer. Among the patients 2.4% of them revealed
familial incidence of gastric cancer. No significant
difference was noticed between cases and controls with
regard to consanguinity (P = 0.473).
CONCLUSION: The Present data suggest that eNOS-786
C/C genotype and C allele may be considered as
potential risk factors in patients with gastric cancer.
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Papers by krishnaveni Devulapalli
synthase -786 T > C promoter polymorphism in the
etiology of gastric cancer.
METHODS: A total of 150 GC patients and 150 control
subjects were included in the study. The information
on demographic features was elicited with an informed
consent from all the patients and control subjects using
a structured questionnaire. Helicobacter pylori (H. pylori )
infectivity status was tested in antral biopsies from all
the subjects by rapid urease test following the method
of Vaira et al . Genomic DNA was isolated from whole
blood samples following the salting out method of Lahiri
et al . Genotype analysis of the rs2070744 polymorphism
was carried out by allele-specific polymerase chain reaction method. The genotypes were determined
based on the appearance of bands on an agarose gel
stained with ethidium bromide under ultraviolet gel
documentation with the help of 100 bp ladder. Odds
ratios and corresponding 95% confidence intervals were
determined using java stat online software.
RESULTS: There was a significant difference in
the distribution of C allele (C vs T ; P = 0.000, OR
= 5.038) in patient group compared to the control
subjects exhibiting a fivefold increased risk for gastric
cancer. When the T/T and C/C genotypes were
compared, there was an enhanced gastric cancer risk
for individuals with C/C genotype (T/T vs C/C ; P =
0.000). Among the demographic factors, smoking and
alcoholism were the common risk factors in patients
compared to the control subjects (P < 0.05). Patients
with smoking and alcoholism developed cancer even
in heterozygous T/C condition (smoking: P = 0.020
and alcoholism: P = 0.005). Individuals with H. pylori
infection showed seven fold increased risk for cancer.
All the patients with C/C genotype revealed a significant
association between H. pylori infection and gastric
cancer. Among the patients 2.4% of them revealed
familial incidence of gastric cancer. No significant
difference was noticed between cases and controls with
regard to consanguinity (P = 0.473).
CONCLUSION: The Present data suggest that eNOS-786
C/C genotype and C allele may be considered as
potential risk factors in patients with gastric cancer.
synthase -786 T > C promoter polymorphism in the
etiology of gastric cancer.
METHODS: A total of 150 GC patients and 150 control
subjects were included in the study. The information
on demographic features was elicited with an informed
consent from all the patients and control subjects using
a structured questionnaire. Helicobacter pylori (H. pylori )
infectivity status was tested in antral biopsies from all
the subjects by rapid urease test following the method
of Vaira et al . Genomic DNA was isolated from whole
blood samples following the salting out method of Lahiri
et al . Genotype analysis of the rs2070744 polymorphism
was carried out by allele-specific polymerase chain reaction method. The genotypes were determined
based on the appearance of bands on an agarose gel
stained with ethidium bromide under ultraviolet gel
documentation with the help of 100 bp ladder. Odds
ratios and corresponding 95% confidence intervals were
determined using java stat online software.
RESULTS: There was a significant difference in
the distribution of C allele (C vs T ; P = 0.000, OR
= 5.038) in patient group compared to the control
subjects exhibiting a fivefold increased risk for gastric
cancer. When the T/T and C/C genotypes were
compared, there was an enhanced gastric cancer risk
for individuals with C/C genotype (T/T vs C/C ; P =
0.000). Among the demographic factors, smoking and
alcoholism were the common risk factors in patients
compared to the control subjects (P < 0.05). Patients
with smoking and alcoholism developed cancer even
in heterozygous T/C condition (smoking: P = 0.020
and alcoholism: P = 0.005). Individuals with H. pylori
infection showed seven fold increased risk for cancer.
All the patients with C/C genotype revealed a significant
association between H. pylori infection and gastric
cancer. Among the patients 2.4% of them revealed
familial incidence of gastric cancer. No significant
difference was noticed between cases and controls with
regard to consanguinity (P = 0.473).
CONCLUSION: The Present data suggest that eNOS-786
C/C genotype and C allele may be considered as
potential risk factors in patients with gastric cancer.