Papers by Dr Shahla Siddiqui
The regression parameters for intercept (a) and slope (b) of the length (L)-Weight (W) relationsh... more The regression parameters for intercept (a) and slope (b) of the length (L)-Weight (W) relationship for the model W = a.L b were determined in Pomadasys maculatum (Bloch 1797) (family: Pomadasyidae). Data on fish size was gathered for 221 male and 231 females fishes collected from Karachi coast and analyzed with correlation and regression to determine relationship of fish weight with standard (SL) and total (TL) lengths in male, female separately and with pooled data for the two sexes. The magnitude of exponents (b) varied only slightly in the regression equations. The t-test for various pairs (male vs. female, male vs. pooled sexes and female vs. pooled sexes) for SL or TL as independent variables indicated the differences among the values of b statistically insignificant at p < 0.05. The regression lines were therefore parallel in this species. The magnitude of b was in all cases near 3 which indicated that that this fish grew symmetrically and isometrically.
Extreme environments merit special attention and significance because of the possible existence o... more Extreme environments merit special attention and significance because of the possible existence of
thermophilic microorganisms in such ecological niches. Keeping this in mind indigenous stove ash samples were
explored for extremophilic bacteria in term of their biodiversity. Accordingly, this study reports 37 bacterial isolates from
the local wood run oven (Tandoor) ash samples. All the isolated strains belong to genus Bacillus on the bases of morphocultural
and biochemical considerations. The average temperature tolerance profile was >45°C thereby, indicating
towards the thermophilic nature of the isolated strains. The Bacillus isolates were screened for 10 different hydrolytic
enzymes (cellulase, xylanase, amylase, pectinase, caseinase, keratinase, lipase, esterase, dextranase and β-galactosidase)
by plate screening method using the medium incorporated with specific substrate(s). It was found that keratinase was
produced by all the isolates while, 36 (97.2%) isolates showed caseinase and esterase production. Amylase was produced
by 35(94.6%) isolates and 34 (91.8%) isolates were able to degrade Tween-80 and xylan as substrate for lipase and
xylanase respectively. The enzyme, β-galactosidase was produced by 31 (89.1%) of the isolates. Cellulase and
dextranase were produced by 26 (70.2%) and 22 (59.4%) isolates respectively. None of the isolates could (under the
existing conditions) produce pectin-hydrolyzing enzyme. According to the tukey’s post hoc test, significant difference
was found between the mean enzyme index of all the (screened) enzymes. Thus, the isolated bacterial strains with
diverse hydrolytic potential may be of great value and relevance for the existing (national) industrial setups.
Keywords: Extracellular, thermophiles, hydrolytic enzyme profile, stove ash samples, enzyme index
Shrimps are very important part of the export economy of Pakistan. They share about 60 % the tota... more Shrimps are very important part of the export economy of Pakistan. They share about 60 % the total exports
of the sea food. For the studies of shrimp health, the length-weight studies are critical for the evaluation of the shrimp
stocks. In this paper an attempt has been made to explore the Length-weight relationships of the shrimps at the creeks
zone of Sindh coast (the study area). Furthermore, the impacts of seasonal change on the growth of shrimps (length and
weight treated as parameter) have been evaluated. The main objective of this study is to analyze the seasonal and
spatial pattern of growth and condition of the selected shrimp species. For this purpose, three most abundant shrimp
species Penaeus indicus, Metapenaeus affinis and Exopalaemon styliferus have been selected out of 30 found at the
coastal creeks areas of Sindh, Pakistan from April 2013 to January 2014. Pre and Post-Monsoon seasonal changes
were analyzed through Geographic Information Science (GIS) and universally accepted equation for LWRs logW =
alogLb were applied on three species selected. It has been found that post-monsoon is the ideal period for shrimp catch
for three species. LWRs (growth pattern) found stable in Penaeus indicus and Metapenaeus affinis while predicting an
alarming deteriorated situation for Exopalaemon styliferus at creeks zone.
ACKNOWLEDGEMENTS
We would like to express our thanks to Mr. Paul
Fanning, Chief Technical Advisor of Food and
Agricultural Organization (FAO) and Marine Fisheries
Department (MFD) and Government of Sindh for the
accessibility of the collected data from the field
surveys. We are also thankful to WWF-Pakistan for the
financial assistance for the execution of the GIS
project. Very special thanks to Ms. Shahla Siddiqui
Cooperative Teacher from Department of Statistics,
University of Karachi for her technical assistance in the
quantitative analysis of data.
As per the estimates of National Interview Health Survey (NHIS) -2012, adult vaccination is exten... more As per the estimates of National Interview Health Survey (NHIS) -2012, adult vaccination is extensively ignored and a very less number of adults could receive recommended vaccine doses. Thus, a cross sectional study was conducted to evaluate the knowledge and attitude towards adult vaccination programs among the adult citizens of Karachi, Pakistan. The survey covered about 54.28% of the total areas of Karachi, Pakistan. A questionnaire comprising of 11 questions was designed and a total of 500 individuals ranging from 11-61 years of age participated in the study. Responses to each question were statistically analyzed and the associations between different variables were established using SPSS 16. According to the results, majority (93.4%) of the individuals considered vaccines safe for health and more than 80% agreed that adult immunization is as necessary as child immunization however, a significant difference (p<0.05) was noticed between the vaccination schedule follow up rates during childhood and adulthood and only a few individuals could receive the recommended adult vaccines. A significant association (p<0.05) was also found between the frequency of infections and the factors that may affect the efficiency of immune system such as sleeping disorders, smoking, persistent depression/anxiety, alcohol intake and the use of anti-depressive drugs/steroids/cortisones. Results showed that a number of reasons may increase the reluctance of people towards any vaccination program such as the cost, risk of quality assurance and requirement of booster doses. Thus, the study emphasizes on the need of awareness programs for adult vaccination as the lack of awareness and ignorance in this sector may render masses susceptible to serious & life threatening infections.
kfbd.giresun.edu.tr
The length, weight and condition factor data were recorded in (224) fishes of Katsuwonus pelamis ... more The length, weight and condition factor data were recorded in (224) fishes of Katsuwonus pelamis in three different (pre-monsoon, monsoon, post-monsoon) seasons of the year (January 2011-December 2011) from the coast of Karachi. The highest mean length (67.5+0.62) were recorded in monsoon season and the lowest mean length (51.25+1.22) was recoded in post-monsoon season. The highest mean weights (3200+40.31) were recorded in pre-monsoon season and the lowest mean weight (1200+131.65) was recorded in postmonsoon season. The highest condition factors (1.14) were recorded in monsoon season.
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Papers by Dr Shahla Siddiqui
thermophilic microorganisms in such ecological niches. Keeping this in mind indigenous stove ash samples were
explored for extremophilic bacteria in term of their biodiversity. Accordingly, this study reports 37 bacterial isolates from
the local wood run oven (Tandoor) ash samples. All the isolated strains belong to genus Bacillus on the bases of morphocultural
and biochemical considerations. The average temperature tolerance profile was >45°C thereby, indicating
towards the thermophilic nature of the isolated strains. The Bacillus isolates were screened for 10 different hydrolytic
enzymes (cellulase, xylanase, amylase, pectinase, caseinase, keratinase, lipase, esterase, dextranase and β-galactosidase)
by plate screening method using the medium incorporated with specific substrate(s). It was found that keratinase was
produced by all the isolates while, 36 (97.2%) isolates showed caseinase and esterase production. Amylase was produced
by 35(94.6%) isolates and 34 (91.8%) isolates were able to degrade Tween-80 and xylan as substrate for lipase and
xylanase respectively. The enzyme, β-galactosidase was produced by 31 (89.1%) of the isolates. Cellulase and
dextranase were produced by 26 (70.2%) and 22 (59.4%) isolates respectively. None of the isolates could (under the
existing conditions) produce pectin-hydrolyzing enzyme. According to the tukey’s post hoc test, significant difference
was found between the mean enzyme index of all the (screened) enzymes. Thus, the isolated bacterial strains with
diverse hydrolytic potential may be of great value and relevance for the existing (national) industrial setups.
Keywords: Extracellular, thermophiles, hydrolytic enzyme profile, stove ash samples, enzyme index
of the sea food. For the studies of shrimp health, the length-weight studies are critical for the evaluation of the shrimp
stocks. In this paper an attempt has been made to explore the Length-weight relationships of the shrimps at the creeks
zone of Sindh coast (the study area). Furthermore, the impacts of seasonal change on the growth of shrimps (length and
weight treated as parameter) have been evaluated. The main objective of this study is to analyze the seasonal and
spatial pattern of growth and condition of the selected shrimp species. For this purpose, three most abundant shrimp
species Penaeus indicus, Metapenaeus affinis and Exopalaemon styliferus have been selected out of 30 found at the
coastal creeks areas of Sindh, Pakistan from April 2013 to January 2014. Pre and Post-Monsoon seasonal changes
were analyzed through Geographic Information Science (GIS) and universally accepted equation for LWRs logW =
alogLb were applied on three species selected. It has been found that post-monsoon is the ideal period for shrimp catch
for three species. LWRs (growth pattern) found stable in Penaeus indicus and Metapenaeus affinis while predicting an
alarming deteriorated situation for Exopalaemon styliferus at creeks zone.
ACKNOWLEDGEMENTS
We would like to express our thanks to Mr. Paul
Fanning, Chief Technical Advisor of Food and
Agricultural Organization (FAO) and Marine Fisheries
Department (MFD) and Government of Sindh for the
accessibility of the collected data from the field
surveys. We are also thankful to WWF-Pakistan for the
financial assistance for the execution of the GIS
project. Very special thanks to Ms. Shahla Siddiqui
Cooperative Teacher from Department of Statistics,
University of Karachi for her technical assistance in the
quantitative analysis of data.
thermophilic microorganisms in such ecological niches. Keeping this in mind indigenous stove ash samples were
explored for extremophilic bacteria in term of their biodiversity. Accordingly, this study reports 37 bacterial isolates from
the local wood run oven (Tandoor) ash samples. All the isolated strains belong to genus Bacillus on the bases of morphocultural
and biochemical considerations. The average temperature tolerance profile was >45°C thereby, indicating
towards the thermophilic nature of the isolated strains. The Bacillus isolates were screened for 10 different hydrolytic
enzymes (cellulase, xylanase, amylase, pectinase, caseinase, keratinase, lipase, esterase, dextranase and β-galactosidase)
by plate screening method using the medium incorporated with specific substrate(s). It was found that keratinase was
produced by all the isolates while, 36 (97.2%) isolates showed caseinase and esterase production. Amylase was produced
by 35(94.6%) isolates and 34 (91.8%) isolates were able to degrade Tween-80 and xylan as substrate for lipase and
xylanase respectively. The enzyme, β-galactosidase was produced by 31 (89.1%) of the isolates. Cellulase and
dextranase were produced by 26 (70.2%) and 22 (59.4%) isolates respectively. None of the isolates could (under the
existing conditions) produce pectin-hydrolyzing enzyme. According to the tukey’s post hoc test, significant difference
was found between the mean enzyme index of all the (screened) enzymes. Thus, the isolated bacterial strains with
diverse hydrolytic potential may be of great value and relevance for the existing (national) industrial setups.
Keywords: Extracellular, thermophiles, hydrolytic enzyme profile, stove ash samples, enzyme index
of the sea food. For the studies of shrimp health, the length-weight studies are critical for the evaluation of the shrimp
stocks. In this paper an attempt has been made to explore the Length-weight relationships of the shrimps at the creeks
zone of Sindh coast (the study area). Furthermore, the impacts of seasonal change on the growth of shrimps (length and
weight treated as parameter) have been evaluated. The main objective of this study is to analyze the seasonal and
spatial pattern of growth and condition of the selected shrimp species. For this purpose, three most abundant shrimp
species Penaeus indicus, Metapenaeus affinis and Exopalaemon styliferus have been selected out of 30 found at the
coastal creeks areas of Sindh, Pakistan from April 2013 to January 2014. Pre and Post-Monsoon seasonal changes
were analyzed through Geographic Information Science (GIS) and universally accepted equation for LWRs logW =
alogLb were applied on three species selected. It has been found that post-monsoon is the ideal period for shrimp catch
for three species. LWRs (growth pattern) found stable in Penaeus indicus and Metapenaeus affinis while predicting an
alarming deteriorated situation for Exopalaemon styliferus at creeks zone.
ACKNOWLEDGEMENTS
We would like to express our thanks to Mr. Paul
Fanning, Chief Technical Advisor of Food and
Agricultural Organization (FAO) and Marine Fisheries
Department (MFD) and Government of Sindh for the
accessibility of the collected data from the field
surveys. We are also thankful to WWF-Pakistan for the
financial assistance for the execution of the GIS
project. Very special thanks to Ms. Shahla Siddiqui
Cooperative Teacher from Department of Statistics,
University of Karachi for her technical assistance in the
quantitative analysis of data.