Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich eth... more Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich ethnomedicinal values. The Bodo population of Assam consumes the fruit extract from the plant as a preventative measure against diabetes.Aim of the study: The goal of this study was to investigate metal content, major phytocompounds, α-amylase and α-glucosidase inhibitory activity as well as cytotoxicity properties of Ficus racemosa fruit extracts.Materials and methods: The GC-MS technology was used to analyze the main phytochemical content of the plant and heavy metal detection was performed by Atomic absorption spectrophotometer. Enzyme inhibition was studied by UV/VIS spectrophotometric methods. Furthermore, the druglikeness and bioavailability properties of compounds were carried out using computer-aided tools, SwissADME and admetSAR. Docking and visualization were performed in AutoDock vina and Discovery studio. The latter research has been supported by the pharmacophore modeling and structure superimposition of studied compounds for lead molecules.Results: GC-MS analysis showed six major compounds from the plant. However, the study found that the fruits of Ficus racemosa contain negligible amounts of toxic elements. Biochemical studies found that the fruit of F. racemosa possesses strong α-amylase and α-glucosidase inhibitory properties. All the six compounds identified were predicted to have druglikeness property with high cell membrane permeability and bioavailability. The Docking study showed strong binding affinities between compounds and enzymes. At the same time in-vitro cytotoxicity and apoptosis results on healthy PBMC and splenocytes showed negligible toxicity (5-12%) after 24 h of exposure. Pharmacophore features and structure superimposition revealed compound 3 as a pivot moleculeConclusion: Ficus racemosa fruit extract inhibited α-amylase and α-glucosidase activity indicating its antihyperglycemic properties. Interestingly, negligible cytotoxic effect was seen in healthy PBMC and splenocytes.
Swargiary et al.: Antihyperglycemic Property of Lindernia crustacea Lindernia crustacea (L.) F. M... more Swargiary et al.: Antihyperglycemic Property of Lindernia crustacea Lindernia crustacea (L.) F. Muell is a small herbaceous plant with several ethnomedicinal values. The present study investigated the phytochemical content and alpha-amylase and alpha-glucosidase inhibitory property of Lindernia crustacea. Methanolic crude extract of plant was obtained following the Soxhlet method. The crude extract was studied for alpha-amylase and alpha-glucosidase inhibitory activity. Phytochemical analysis was carried out using the gas chromatography-mass spectrometry technique. Furthermore, docking study was carried out with the phytocompounds to see the binding affinity with the enzymes. In silico drug-likeness and pharmacological properties were also carried out using Swiss absorption, distribution, metabolism and excretion, and absorption, distribution, metabolism, excretion and toxicity lab tools. The plant extracts showed concentration-dependent inhibition of enzyme activities with half-maximal inhibitory concentration values of 3.11 mg/ml and 548.9 µg/ml for alpha-amylase and alphaglucosidase, respectively. Gas chromatography-mass spectrometry study identified ten phytocompounds with molecular weights ranging from 264.4 to 561 g/mol. Docking study showed 1-(4-Hydroxybenzoyl)-6,7-dimethoxyisoquinoline as the best binding compound with the enzymes. Phytocompounds identified from Lindernia crustacea were predicted to have substantial drug-likeness and absorption, distribution, metabolism, excretion and toxicity properties. The enzyme inhibition study and binding interactions of phytocompounds suggest promising alpha-amylase and alpha-glucosidase inhibitory activity of Lindernia crustacea. Therefore, the aerial part of Lindernia crustacea may be further investigated to know the exact mode of biological actions.
Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich eth... more Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich ethnomedicinal values. The Bodo population of Assam consumes the fruit extract from the plant as a preventative measure against diabetes.Aim of the study: The goal of this study was to investigate metal content, major phytocompounds, α-amylase and α-glucosidase inhibitory activity as well as cytotoxicity properties of Ficus racemosa fruit extracts.Materials and methods: The GC-MS technology was used to analyze the main phytochemical content of the plant and heavy metal detection was performed by Atomic absorption spectrophotometer. Enzyme inhibition was studied by UV/VIS spectrophotometric methods. Furthermore, the druglikeness and bioavailability properties of compounds were carried out using computer-aided tools, SwissADME and admetSAR. Docking and visualization were performed in AutoDock vina and Discovery studio. The latter research has been supported by the pharmacophore modeling and s...
Combinatorial Chemistry & High Throughput Screening, 2021
Background:α-Amylase and α-glucosidase inhibitors are widely used to suppress postprandial glycem... more Background:α-Amylase and α-glucosidase inhibitors are widely used to suppress postprandial glycemia in the treatment of type-2 diabetes.Objectives:To evaluate the metallic content, phytocompounds, and α-amylase and α-glucosidase inhibitory activity of Musa balbisiana rhizome using in-vitro and in-silico methods.Materials and Methods:Heavy metal content was detected by AAS following standard protocol. Major phytochemicals of the plant were analyzed by GC-MS technique. Enzyme inhibition study was carried out by UV/VIS spectrophotometric methods. The drug-likeness and bio-availability properties of major compounds were carried out using computer-aided tools – SwissADME and ADMElab. Docking and visualization were performed in AutoDock vina and Discovery studio tools.Results:The study found that the fruits of M. balbisiana contain a negligible amount of toxic elements. GC-MS analysis showed five major compounds from the rhizome of M. balbisiana. Invitro enzyme assays revealed strong α-am...
The present investigation is an attempt to isolate and identify the yeasts used by some local abo... more The present investigation is an attempt to isolate and identify the yeasts used by some local aborigines of Arunachal Pradesh and observe their behaviour under in-vitro conditions. Interestingly, all the isolates have shown a preference of growth at neutral pH; a character not usually observed in yeasts who generally prefer an acidic medium for growth. The results obtained in the present findings suggest that all the isolates are different in terms of their physiological or biochemical activities. One important aspect of the studies was the successful isolation of pure cultures from a consortium whose contents were previously unknown (Sarma, 2002). Protein profiling of the isolates have revealed that the isolates are indeed variants at the genetic level as was evident from expression of whole cell proteins. The isolates might therefore be mutagenic variants, which arose during segregation through time immemorial. It can be predicted that careful identification and selection of yeast...
Hydrocotyle sibthorpioides Lam. is a popular medicinal plant of Assam having several ethnomedicin... more Hydrocotyle sibthorpioides Lam. is a popular medicinal plant of Assam having several ethnomedicinal values. The present study investigated the metallic content, phytochemical contents, α-amylase, and α-glucosidase enzymes inhibitory property of H. sibthorpioides using in-vitro and in-silico methods. Heavy metal contents were analyzed using Atomic Absorption Spectroscopy. GC–MS was used to analyze the phytochemical compounds of the plant. Enzyme inhibition study was carried out by Spectrophotometry methods. The drug-likeness and toxicity properties of the phytocompounds were studied using SwissADME and ADMETlab databases. Docking and molecular visualizations were performed in AutoDock vina and Discovery studio tools. The study found that the extract of H. sibthorpioides contains a negligible amount of toxic elements. GC–MS analysis detected four compounds from the methanolic extract of the plant. Biochemical study showed considerable α-amylase and α-glucosidase enzyme inhibitory prop...
Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla... more Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla is an important plant native to India and many other Asian countries. Parts of this plant such as seeds, fruit pulp, inflorescence, pseudo-stem, and corm have been known to possess several medicinal values. In Kokrajhar district of Assam, the decoction of corm part of the plant is traditionally used as antidiabetic medicine. The present study was aimed to investigate the phytochemicals, antioxidants, trace element, and major compounds of the corm part of Musa balbisiana. Phenolic and flavonoid contents were estimated following standard protocols. The antioxidant activity of the plant was studied by ferric reducing antioxidant power assay, total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl, and lipid peroxidation scavenging assay. The phytochemical study revealed that the corm extract is rich in protein, carbohydrates, phenolics, and flavonoid content. Antioxidant study revealed strong free radical scavenging property of the crude corm extract of Musa balbisiana. The elemental analysis showed highest Zn content (0.2993 ppm) followed by Ni, Cu, and Mn. Pb, Cd, and Cr were not detected in the extract. GC-MS analysis showed difluroisocyanotophosphine to be the major compound of Musa balbisiana corm extract
Background: The use of medicinal plants for general wellbeing and disease treatment is a common p... more Background: The use of medicinal plants for general wellbeing and disease treatment is a common practice among tribal communities of Kokrajhar districts of Assam. However, little works have been done to study the pharmacological aspect of the plants. Objectives: The present study intends to study the antioxidant and antiproliferative properties of selected medicinal plants used by the tribal communities of Kokrajhar district of Assam since ancient times. Methods: Five traditionally important medicinal plants namely, Cassia fistula, Citrus grandis, Lindernia crustacea, Sacciolepis myosuroides, and Zingiber zerumbet were investigated for antioxidant, antiproliferative (cytotoxic) and apoptosis-inducing potential in the malignant cancer cell line. Phytochemical content such as phenolic and flavonoid content were estimated following standard protocol. The methanolic extract of plants were investigated following phosphomolybdate method (TAC), FRAP, DPPH, ABTS, and TBARS assays. Antiproli...
Background The practice of ethnomedicine remains to be the primary source of healthcare in many p... more Background The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. Objective The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Methods Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio software....
Ethnobotanical knowledge has been the backbone of rural healthcare since ancient times. Many dise... more Ethnobotanical knowledge has been the backbone of rural healthcare since ancient times. Many diseases including helminthiasis are cured by traditional medicine in many parts of the world. The present study aims at exploring the ethnobotanicals used as anthelmintic medicines by the tribal communities of Chirang district of Assam. The present study was conducted in different villages under Chirang district of Assam, India. A face-to-face interview was carried out during survey work along with readymade questionnaire. In our survey work, 20 neighbouring villages were taken as a single cluster and one sample informant was collected. Information regarding the plant and plant parts used, methodology of use as well as informant demography such as age, sex, education was also collected. A total of 20 villages were surveyed and information was gathered from 27 informants, 23 kaviraja and 4 elderly people, 15 male and 12 female. The information collected revealed 43 medicinal plants belonging...
Clerodendrum viscosum, Eryngium foetidum, Lippia javanica, and Murraya koenigii are one among the... more Clerodendrum viscosum, Eryngium foetidum, Lippia javanica, and Murraya koenigii are one among the common wild edible plants in Northeast India which are also used as antidiabetic, stomach-ache relieving drugs, etc., The present study was aimed to reveal the phytochemical, antioxidant, and anthelmintic activity of the plants. The antioxidant capacity of methanolic extract of plants was studied by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power, TBARS, and total antioxidant activity (TAA). Total phenolics, flavonoids, Vitamin C, carbohydrate, and protein are also estimated following standard protocols. Anthelmintic activity of the extracts has also been studied in vitro against trematode parasites. The result showed that the methanolic extracts of plants possess a substantial quantity of alkaloids, phenolics, flavonoids, proteins, carbohydrates, and Vitamin C. Phenolics, flavonoids, and Vitamin C contents were found higher in C. viscosum followed by M. koenigii, L. javanica, and E. foetidum. The in vitro antioxidant assays revealed substantial free radical scavenging property in all the plants. TAA increased in the order C. viscosum > M. koenigii > L. javanica > E. foetidum. Similarly, C. viscosum displayed a better antioxidant capacity with IC50 values 29.74 ± 3.63 μg and 148.77 ± 18.38 μg for DPPH and thiobarbituric acid reactive species, respectively. In addition, the plant extracts also showed good anthelmintic activity against Paramphistomum sp. Time taken for paralysis and death were 0:56 ± 0:09 h and 1:35 ± 0:07 h for L. javanica at 50 mg/mL concentration. The study therefore suggests the importance of tested plants as a natural source of free radical scavenger and plausible veterinary uses.
Background: The practice of ethnomedicine remains to be the primary source of healthcare in many ... more Background: The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. Objective: The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Methods: Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio softwa...
Background: The practice of ethnomedicine remains to be the primary source of healthcare in many ... more Background: The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. Objective: The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Methods: Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio softwa...
Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla... more Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla is an important plant native to India and many other Asian countries. Parts of this plant such as seeds, fruit pulp, inflorescence, pseudo-stem, and corm have been known to possess several medicinal values. In Kokrajhar district of Assam, the decoction of corm part of the plant is traditionally used as antidiabetic medicine. The present study was aimed to investigate the phytochemicals, antioxidants, trace element, and major compounds of the corm part of Musa balbisiana. Phenolic and flavonoid contents were estimated following standard protocols. The antioxidant activity of the plant was studied by ferric reducing antioxidant power assay, total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl, and lipid peroxidation scavenging assay. The phytochemical study revealed that the corm extract is rich in protein, carbohydrates, phenolics, and flavonoid content. Antioxidant study revealed strong free radical scavenging property of the crude corm extract of Musa balbisiana. The elemental analysis showed highest Zn content (0.2993 ppm) followed by Ni, Cu, and Mn. Pb, Cd, and Cr were not detected in the extract. GC-MS analysis showed difluroisocyanotophosphine to be the major compound of Musa balbisiana corm extract
Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich eth... more Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich ethnomedicinal values. The Bodo population of Assam consumes the fruit extract from the plant as a preventative measure against diabetes.Aim of the study: The goal of this study was to investigate metal content, major phytocompounds, α-amylase and α-glucosidase inhibitory activity as well as cytotoxicity properties of Ficus racemosa fruit extracts.Materials and methods: The GC-MS technology was used to analyze the main phytochemical content of the plant and heavy metal detection was performed by Atomic absorption spectrophotometer. Enzyme inhibition was studied by UV/VIS spectrophotometric methods. Furthermore, the druglikeness and bioavailability properties of compounds were carried out using computer-aided tools, SwissADME and admetSAR. Docking and visualization were performed in AutoDock vina and Discovery studio. The latter research has been supported by the pharmacophore modeling and structure superimposition of studied compounds for lead molecules.Results: GC-MS analysis showed six major compounds from the plant. However, the study found that the fruits of Ficus racemosa contain negligible amounts of toxic elements. Biochemical studies found that the fruit of F. racemosa possesses strong α-amylase and α-glucosidase inhibitory properties. All the six compounds identified were predicted to have druglikeness property with high cell membrane permeability and bioavailability. The Docking study showed strong binding affinities between compounds and enzymes. At the same time in-vitro cytotoxicity and apoptosis results on healthy PBMC and splenocytes showed negligible toxicity (5-12%) after 24 h of exposure. Pharmacophore features and structure superimposition revealed compound 3 as a pivot moleculeConclusion: Ficus racemosa fruit extract inhibited α-amylase and α-glucosidase activity indicating its antihyperglycemic properties. Interestingly, negligible cytotoxic effect was seen in healthy PBMC and splenocytes.
Swargiary et al.: Antihyperglycemic Property of Lindernia crustacea Lindernia crustacea (L.) F. M... more Swargiary et al.: Antihyperglycemic Property of Lindernia crustacea Lindernia crustacea (L.) F. Muell is a small herbaceous plant with several ethnomedicinal values. The present study investigated the phytochemical content and alpha-amylase and alpha-glucosidase inhibitory property of Lindernia crustacea. Methanolic crude extract of plant was obtained following the Soxhlet method. The crude extract was studied for alpha-amylase and alpha-glucosidase inhibitory activity. Phytochemical analysis was carried out using the gas chromatography-mass spectrometry technique. Furthermore, docking study was carried out with the phytocompounds to see the binding affinity with the enzymes. In silico drug-likeness and pharmacological properties were also carried out using Swiss absorption, distribution, metabolism and excretion, and absorption, distribution, metabolism, excretion and toxicity lab tools. The plant extracts showed concentration-dependent inhibition of enzyme activities with half-maximal inhibitory concentration values of 3.11 mg/ml and 548.9 µg/ml for alpha-amylase and alphaglucosidase, respectively. Gas chromatography-mass spectrometry study identified ten phytocompounds with molecular weights ranging from 264.4 to 561 g/mol. Docking study showed 1-(4-Hydroxybenzoyl)-6,7-dimethoxyisoquinoline as the best binding compound with the enzymes. Phytocompounds identified from Lindernia crustacea were predicted to have substantial drug-likeness and absorption, distribution, metabolism, excretion and toxicity properties. The enzyme inhibition study and binding interactions of phytocompounds suggest promising alpha-amylase and alpha-glucosidase inhibitory activity of Lindernia crustacea. Therefore, the aerial part of Lindernia crustacea may be further investigated to know the exact mode of biological actions.
Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich eth... more Ethnobotanical relevance: Ficus racemosa L. is one of Assam's best-known plants with rich ethnomedicinal values. The Bodo population of Assam consumes the fruit extract from the plant as a preventative measure against diabetes.Aim of the study: The goal of this study was to investigate metal content, major phytocompounds, α-amylase and α-glucosidase inhibitory activity as well as cytotoxicity properties of Ficus racemosa fruit extracts.Materials and methods: The GC-MS technology was used to analyze the main phytochemical content of the plant and heavy metal detection was performed by Atomic absorption spectrophotometer. Enzyme inhibition was studied by UV/VIS spectrophotometric methods. Furthermore, the druglikeness and bioavailability properties of compounds were carried out using computer-aided tools, SwissADME and admetSAR. Docking and visualization were performed in AutoDock vina and Discovery studio. The latter research has been supported by the pharmacophore modeling and s...
Combinatorial Chemistry & High Throughput Screening, 2021
Background:α-Amylase and α-glucosidase inhibitors are widely used to suppress postprandial glycem... more Background:α-Amylase and α-glucosidase inhibitors are widely used to suppress postprandial glycemia in the treatment of type-2 diabetes.Objectives:To evaluate the metallic content, phytocompounds, and α-amylase and α-glucosidase inhibitory activity of Musa balbisiana rhizome using in-vitro and in-silico methods.Materials and Methods:Heavy metal content was detected by AAS following standard protocol. Major phytochemicals of the plant were analyzed by GC-MS technique. Enzyme inhibition study was carried out by UV/VIS spectrophotometric methods. The drug-likeness and bio-availability properties of major compounds were carried out using computer-aided tools – SwissADME and ADMElab. Docking and visualization were performed in AutoDock vina and Discovery studio tools.Results:The study found that the fruits of M. balbisiana contain a negligible amount of toxic elements. GC-MS analysis showed five major compounds from the rhizome of M. balbisiana. Invitro enzyme assays revealed strong α-am...
The present investigation is an attempt to isolate and identify the yeasts used by some local abo... more The present investigation is an attempt to isolate and identify the yeasts used by some local aborigines of Arunachal Pradesh and observe their behaviour under in-vitro conditions. Interestingly, all the isolates have shown a preference of growth at neutral pH; a character not usually observed in yeasts who generally prefer an acidic medium for growth. The results obtained in the present findings suggest that all the isolates are different in terms of their physiological or biochemical activities. One important aspect of the studies was the successful isolation of pure cultures from a consortium whose contents were previously unknown (Sarma, 2002). Protein profiling of the isolates have revealed that the isolates are indeed variants at the genetic level as was evident from expression of whole cell proteins. The isolates might therefore be mutagenic variants, which arose during segregation through time immemorial. It can be predicted that careful identification and selection of yeast...
Hydrocotyle sibthorpioides Lam. is a popular medicinal plant of Assam having several ethnomedicin... more Hydrocotyle sibthorpioides Lam. is a popular medicinal plant of Assam having several ethnomedicinal values. The present study investigated the metallic content, phytochemical contents, α-amylase, and α-glucosidase enzymes inhibitory property of H. sibthorpioides using in-vitro and in-silico methods. Heavy metal contents were analyzed using Atomic Absorption Spectroscopy. GC–MS was used to analyze the phytochemical compounds of the plant. Enzyme inhibition study was carried out by Spectrophotometry methods. The drug-likeness and toxicity properties of the phytocompounds were studied using SwissADME and ADMETlab databases. Docking and molecular visualizations were performed in AutoDock vina and Discovery studio tools. The study found that the extract of H. sibthorpioides contains a negligible amount of toxic elements. GC–MS analysis detected four compounds from the methanolic extract of the plant. Biochemical study showed considerable α-amylase and α-glucosidase enzyme inhibitory prop...
Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla... more Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla is an important plant native to India and many other Asian countries. Parts of this plant such as seeds, fruit pulp, inflorescence, pseudo-stem, and corm have been known to possess several medicinal values. In Kokrajhar district of Assam, the decoction of corm part of the plant is traditionally used as antidiabetic medicine. The present study was aimed to investigate the phytochemicals, antioxidants, trace element, and major compounds of the corm part of Musa balbisiana. Phenolic and flavonoid contents were estimated following standard protocols. The antioxidant activity of the plant was studied by ferric reducing antioxidant power assay, total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl, and lipid peroxidation scavenging assay. The phytochemical study revealed that the corm extract is rich in protein, carbohydrates, phenolics, and flavonoid content. Antioxidant study revealed strong free radical scavenging property of the crude corm extract of Musa balbisiana. The elemental analysis showed highest Zn content (0.2993 ppm) followed by Ni, Cu, and Mn. Pb, Cd, and Cr were not detected in the extract. GC-MS analysis showed difluroisocyanotophosphine to be the major compound of Musa balbisiana corm extract
Background: The use of medicinal plants for general wellbeing and disease treatment is a common p... more Background: The use of medicinal plants for general wellbeing and disease treatment is a common practice among tribal communities of Kokrajhar districts of Assam. However, little works have been done to study the pharmacological aspect of the plants. Objectives: The present study intends to study the antioxidant and antiproliferative properties of selected medicinal plants used by the tribal communities of Kokrajhar district of Assam since ancient times. Methods: Five traditionally important medicinal plants namely, Cassia fistula, Citrus grandis, Lindernia crustacea, Sacciolepis myosuroides, and Zingiber zerumbet were investigated for antioxidant, antiproliferative (cytotoxic) and apoptosis-inducing potential in the malignant cancer cell line. Phytochemical content such as phenolic and flavonoid content were estimated following standard protocol. The methanolic extract of plants were investigated following phosphomolybdate method (TAC), FRAP, DPPH, ABTS, and TBARS assays. Antiproli...
Background The practice of ethnomedicine remains to be the primary source of healthcare in many p... more Background The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. Objective The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Methods Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio software....
Ethnobotanical knowledge has been the backbone of rural healthcare since ancient times. Many dise... more Ethnobotanical knowledge has been the backbone of rural healthcare since ancient times. Many diseases including helminthiasis are cured by traditional medicine in many parts of the world. The present study aims at exploring the ethnobotanicals used as anthelmintic medicines by the tribal communities of Chirang district of Assam. The present study was conducted in different villages under Chirang district of Assam, India. A face-to-face interview was carried out during survey work along with readymade questionnaire. In our survey work, 20 neighbouring villages were taken as a single cluster and one sample informant was collected. Information regarding the plant and plant parts used, methodology of use as well as informant demography such as age, sex, education was also collected. A total of 20 villages were surveyed and information was gathered from 27 informants, 23 kaviraja and 4 elderly people, 15 male and 12 female. The information collected revealed 43 medicinal plants belonging...
Clerodendrum viscosum, Eryngium foetidum, Lippia javanica, and Murraya koenigii are one among the... more Clerodendrum viscosum, Eryngium foetidum, Lippia javanica, and Murraya koenigii are one among the common wild edible plants in Northeast India which are also used as antidiabetic, stomach-ache relieving drugs, etc., The present study was aimed to reveal the phytochemical, antioxidant, and anthelmintic activity of the plants. The antioxidant capacity of methanolic extract of plants was studied by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power, TBARS, and total antioxidant activity (TAA). Total phenolics, flavonoids, Vitamin C, carbohydrate, and protein are also estimated following standard protocols. Anthelmintic activity of the extracts has also been studied in vitro against trematode parasites. The result showed that the methanolic extracts of plants possess a substantial quantity of alkaloids, phenolics, flavonoids, proteins, carbohydrates, and Vitamin C. Phenolics, flavonoids, and Vitamin C contents were found higher in C. viscosum followed by M. koenigii, L. javanica, and E. foetidum. The in vitro antioxidant assays revealed substantial free radical scavenging property in all the plants. TAA increased in the order C. viscosum > M. koenigii > L. javanica > E. foetidum. Similarly, C. viscosum displayed a better antioxidant capacity with IC50 values 29.74 ± 3.63 μg and 148.77 ± 18.38 μg for DPPH and thiobarbituric acid reactive species, respectively. In addition, the plant extracts also showed good anthelmintic activity against Paramphistomum sp. Time taken for paralysis and death were 0:56 ± 0:09 h and 1:35 ± 0:07 h for L. javanica at 50 mg/mL concentration. The study therefore suggests the importance of tested plants as a natural source of free radical scavenger and plausible veterinary uses.
Background: The practice of ethnomedicine remains to be the primary source of healthcare in many ... more Background: The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. Objective: The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Methods: Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio softwa...
Background: The practice of ethnomedicine remains to be the primary source of healthcare in many ... more Background: The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. Objective: The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Methods: Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio softwa...
Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla... more Daimari et al.: Phytochemical investigation of Musa balbisiana corm extract Musa balbisiana colla is an important plant native to India and many other Asian countries. Parts of this plant such as seeds, fruit pulp, inflorescence, pseudo-stem, and corm have been known to possess several medicinal values. In Kokrajhar district of Assam, the decoction of corm part of the plant is traditionally used as antidiabetic medicine. The present study was aimed to investigate the phytochemicals, antioxidants, trace element, and major compounds of the corm part of Musa balbisiana. Phenolic and flavonoid contents were estimated following standard protocols. The antioxidant activity of the plant was studied by ferric reducing antioxidant power assay, total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl, and lipid peroxidation scavenging assay. The phytochemical study revealed that the corm extract is rich in protein, carbohydrates, phenolics, and flavonoid content. Antioxidant study revealed strong free radical scavenging property of the crude corm extract of Musa balbisiana. The elemental analysis showed highest Zn content (0.2993 ppm) followed by Ni, Cu, and Mn. Pb, Cd, and Cr were not detected in the extract. GC-MS analysis showed difluroisocyanotophosphine to be the major compound of Musa balbisiana corm extract
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