Background: Severe COVID-19 has been proposed to represent a form of viral sepsis. The prevalence... more Background: Severe COVID-19 has been proposed to represent a form of viral sepsis. The prevalence of sepsis due to this disease has not been evaluated. Some patients with severe COVID-19 present SARS-CoV-2 antigenemia. Whether antigenemia constitutes a factor associated to viral sepsis and fatal outcome in these patients is unknown. Methods: This study recruited 400 patients hospitalized with COVID-19. Prevalence of sepsis in the first 24 hours following hospitalization was determined using the SEPSIS-3 criteria. Prevalence and concentration of SARS-CoV-2 N-antigenemia was determined in plasma at hospitalization using the Panbio® COVID-19 Ag Rapid Test from Abbott and the COV-QUANTO® ELISA from AAZ. Findings: The prevalence of sepsis due to SARS-CoV-2 infection at hospitalization was of 54%, affecting mostly to the respiratory, renal and coagulation function. Antigenemia was present in the 53% of the patients, and constituted an independent risk factor of sepsis in the multivariate analysis (OR [CI95%], p): presence of N-antigenemia (Panbio®): (1·6 [1·1 - 2·5],0·023);concentration of N-antigen >575 pg/mL (COV-QUANTO®): (1·9 [1·0 - 3·5], 0·043). Antigenemia was associated with a reduction in the survival mean time of 9·42 and 9·33 days in the 90 first days following hospitalization, based on the PANBIO® and the COV-QUANTO® tests respectively. Interpretation: Half of the COVID-19 patients needing of hospitalization fulfill the criteria of sepsis. Antigenemia is strongly linked to the presence of this condition, and confers a higher risk of mortality. Monitoring the presence of antigenemia at the earlier moments of COVID-19 could help to identify those patients at risk of deterioration. Clinical Trial Registration Details: This was a sub-study of the CIBERES-UCI-COVID project, registered at Clinicaltrials.gov with the identification NCT04457505. Funding Information: Instituto de Salud Carlos III (COV20/00110;CP20/00041;FI20/00278), Fundació La Marató de TV3 (473/C/2021).Declaration of Interests: AT, JME, FB, RA and JFBM have a patent application on SARS-CoV-2 antigenemia. The remainder authors declare no conflicts of interest regarding this work.Ethics Approval Statement: Written or oral informed consent was obtained directly from all patients, or their legal representative, before enrolment. Scientific and ethical approval of the study protocol was obtained from the respective scientific committees for clinical research of the participant hospitals.
Archivos Latinoamericanos De Nutricion, Jun 1, 2000
The purpose of this study is to obtain a low cholesterol egg powder for the preparation of differ... more The purpose of this study is to obtain a low cholesterol egg powder for the preparation of different foods for persons whose egg consumption is restricted. Egg white and yolk mixtures prepared in different proportions were dehydrated; the following dried mixtures were obtained: A (1:1), B (2:1) and C (3:1) of egg white:yolk respectively. These mixtures were evaluated using the following parameters: proximal analysis, microbiological assay and protein quality evaluation. Physical characteristics of the powder and the sensorial tests of foods prepared with these mixtures were carried out. The fat and the cholesterol content in the mixture C were decreased by 40% and 20% respectively. The microbiological tests showed that the three mixtures were safe for human consumption. The PER of sample A (whole egg) was 3.65 and for the mixture C 3:1 egg white:yolk was 3.05. The PER of the 50:50 protein mixtures eggs white and yolk: with corn lime treated flour (HMN) were higher than that of the casein standard. The sensorial tests of the foods prepared with all the mixtures were acceptable.
Tuberculosis dynamic in Mexico and the world is forcing to readdress the procedures against this ... more Tuberculosis dynamic in Mexico and the world is forcing to readdress the procedures against this disease. In this sense the molecular diagnosis by the acid nucleic amplification from Mycobacteria by the polymerase chain reaction, is been pointing as a technique with certain success in countries with medium and high prevalence of the disease. So the main goal of this work is to evaluate utility of the diagnostic of tuberculosis by the PCR technique, amplifying the rDNA16s marker, from sputum samples from patients with suspicious of tuberculosis. The results show that from 107 clinical samples analyzed, 36 were positive to PCR and 37 were acid fast bacilli smear, the sensibility and specificity of the procedure were 80 and 90% respectively and the positive and negative predictive value was 80 and 88%, the average time for the development of the procedure was 16 hrs. Despite that this is a preliminary study, our results let us to claim the utility of this fast and confidence diagnostic procedure, as an important tool against TB.
Background: Skills in aseptic technique are essential competencies for preventing infection; yet,... more Background: Skills in aseptic technique are essential competencies for preventing infection; yet, aseptic technique is a difficult concept to master. During urinary catheter insertion, failure to properly adhere to strict aseptic technique has been linked to infections and sepsis. This pilot study used simulation to identify the most common breaches in aseptic technique during urinary catheter insertion. Methods: Baccalaureate nursing students with prior documentation of competency were recruited to demonstrate performance of urinary catheterization on a task trainer. The procedure was recorded, and breaches in technique were identified through review of the digital recordings. Results: Data were available for 13 participants. Participants ranged in age from 21 to 43 years (mean, 26.6). Ten participants (77%) were female and three (23%) were male. The participants' mean selfrating of confidence was 3.6 on a 5-point scale (range, 3-5), indicating some confidence in performing the skill. Examination of the video recorded data showed that 10 participants (77%) breached aseptic technique in at least one category and in some instances several categories. The most common breach was cleansing the meatus; seven participants (54%) performed it incorrectly. Conclusions: One-time competency validation using a simulator is not enough to ensure skill mastery or retention. Urinary catheter insertion is a high-risk skill and therefore should be afforded ample time to practice. Innovative approaches to ensure skill mastery, including urinary catheterization and other skills requiring sterile techniques, are needed.
Purpose. The purpose of this study was to evaluate medications that cancer survivors with oxalipl... more Purpose. The purpose of this study was to evaluate medications that cancer survivors with oxaliplatin-induced peripheral neuropathy take to control neuropathic symptom, and to explore self-management techniques used at home to provide temporary relief of painful neuropathy. This was a mixed methods, descriptive, cross-sectional study using self-reported data from colorectal cancer survivors previously treated with oxaliplatin. We analyzed demographic and medication data obtained from participants, along with written comments from an open-ended question regarding methods participants had tried to self-manage symptoms of neuropathy. Results. Twenty-nine percent of the sample reported taking some type of nutritional supplement with potential neuroprotective qualities. Opioids were being taken by 10% of the sample, and nonsteroidal anti-inflammatory and over-the-counter medications were taken by 15% of participants. Twelve percent of participants were taking antidepressants and 10% were...
The use of quartz tuning fork sensors as probes for scanning probe microscopy is growing in popul... more The use of quartz tuning fork sensors as probes for scanning probe microscopy is growing in popularity. Working in shear mode, some methods achieve a lateral resolution comparable with that obtained with standard cantilevered probes, but only in experiments conducted in air or vacuum. Here, we report a method to produce and use commercial AFM tips in electrically driven quartz tuning fork sensors operating in shear mode in a liquid environment. The process is based on attaching a standard AFM tip to the end of a fiber probe which has previously been sharpened. Only the end of the probe is immersed in the buffer solution during imaging. The lateral resolution achieved is about 6 times higher than that of the etched microfiber on its own.
This paper describes an experiment that studies the effect of basic haptic feedback in creating a... more This paper describes an experiment that studies the effect of basic haptic feedback in creating a sense of social interaction within a shared virtual environment (SVE). Although there have been a number of studies investigating the effect of haptic feedback on collaborative task performance, they do not address the effect it has in inducing social presence. The purpose of this experiment is to show that haptic feedback enhances the sense of social presence within a mediated environment. An experiment was carried out using a shared desktop based virtual environment where 20 remotely located couples who did not know one another had to solve a puzzle together. In 10 groups they had shared haptic communication through their hands, and in another group they did not. Hence the haptic feedback was not used for completing the task itself, but rather as a means of social interacting-communicating with the other participant. The results suggest that basic haptic feedback increases the sense of social presence within the shared VE.
Based on the structural style and physiographic criteria, the Central Andes of Peru can be divide... more Based on the structural style and physiographic criteria, the Central Andes of Peru can be divided into segments running parallel to the Pacific coast. The westernmost segment, the Coastal Belt, consists of a Late Jurassic-Cretaceous volcanic arc sequence that was accreted to the South American craton in Cretaceous times. The Mesozoic strata of the adjacent Western Cordillera represent an ENE-vergent fold-and-thrust belt that formed in Eocene times. Tight upright folds developed above a shallow detachment horizon in the West, while more open folds formed above a deeper detachment horizon towards the East and in the neighboring Central Highlands. A completely different style with steeply dipping reverse faults and open folds affecting the Neoproterozoic crystalline basement is typical for the Eastern Cordillera. The Subandean Zone is characterized by mainly NE-vergent imbricate thrusting which occurred in Neogene times. A quantitative estimate of the shortening of the orogen obtained from balanced cross-sections indicates a total shortening of 120-150 km (24%-27%). This shortening was coevel with the Neogene westward drift of South America, occurred at rates between 3 and 4.7 mm/year and was responsible for the high elevation of the Peruvian Andes.
This paper examines the effects of lender and borrower personal characteristics (perceived attrac... more This paper examines the effects of lender and borrower personal characteristics (perceived attractiveness, age and gender) on online peer-to-peer lending decisions. The extant research in finance, marketing, and psychology provides substantial support for the "beauty premium" effect, where ceteris paribus, more (as opposed to less) attractive individuals are favored (e.g., Ravina 2012). Our results qualify these findings, suggesting that (1) when perceived age is a clear signal of competence (college-age signals low competence, while middle age signals significant competence), attractiveness has no effect on loan success; (2) when lender and borrower are of the same gender, attractiveness may actually hurt one’s chances to secure a loan (we observe "beauty is beastly" effect), and (3) loan success is sensitive to the relative age and attractiveness of lenders and borrowers.
Thanks to Christopher James, Anthony Saunders, Allen Berger, Joel Houston, Lars Norden, Andy Nara... more Thanks to Christopher James, Anthony Saunders, Allen Berger, Joel Houston, Lars Norden, Andy Naranjo, Mahendrarajah Nimalendran, and seminar participants at the University of Florida, Financial Management Association and Eastern Finance Association for helpful ...
Quartz tuning fork devices are increasingly being used as nanosensors in Scanning Probe Microscop... more Quartz tuning fork devices are increasingly being used as nanosensors in Scanning Probe Microscopy. They offer some benefits with respect to standard microfabricated cantilevers in certain experimental setups including the study of biomolecules under physiological conditions. In this work, we compare three different working modes for imaging micropatterned antibodies with quartz tuning fork sensors: apart from the classical amplitude and frequency modulation strategies, for first time the jumping mode is implemented using tuning forks. Our results show that the molecules suffer less degradation when working in the jumping mode, due to the reduction of the interaction forces.
Angiotensin I converting enzyme (ACE) was studied in Vero cells, rabbit corneal fibroblasts, and ... more Angiotensin I converting enzyme (ACE) was studied in Vero cells, rabbit corneal fibroblasts, and rabbit corneal endothelial cells. The enzyme activity was determined by means of an assay employing hippuryl-glycyl-glycine as a substrate. The hippuric acid end product was separated from the substrate by reversed phase liquid chromatography and measured spectrophotometrically at 228 nm. The enzyme was further characterized by a captopril inhibition study. Significant ACE activity was found in rabbit corneal endothelial cells but not in other types of cells tested. This is the first report of the presence of this enzyme in a specific ocular cell type and suggests that angiotensin II may play a role in normal ocular physiology.
A 27-year-old woman of Moldavian origin presented at the age of 15 with progressive proximal limb... more A 27-year-old woman of Moldavian origin presented at the age of 15 with progressive proximal limb weakness and painful cramps in her calf muscles. Clinical examination revealed prominent muscle weakness in proximal muscles of the lower extremities and distal anterior compartment of legs, and mild weakness in shoulder girdle muscles. In addition, she had marked calf hypertrophy, muscle atrophy involving the anterior and posterior compartments of the thighs, and the distal anterior compartment of legs, as well as mild scapular winging and hyperlordosis. A muscle biopsy taken from the biceps brachii showed mild dystrophic changes, absent vacuoles, and abundant lobulated fibers. Immunofluorescence and Western blot assays demonstrated complete telethonin deficiency. Molecular analysis revealed a homozygous Trp25X mutation in the telethonin (TCAP) gene resulting in termination of transcription at an early point. Four families from Brazil with telethonin deficiency have previously been reported and classified as LGMD2G, but the actual frequency of this disease is unknown. With this current identification of a case outside the Brazilian population, telethonin mutation-associated LGMD should be considered worldwide.
Desminopathy represents a subgroup of myofibrillar myopathies caused by mutations in the desmin g... more Desminopathy represents a subgroup of myofibrillar myopathies caused by mutations in the desmin gene. Three novel disease-associated mutations in the desmin gene were identified in unrelated Spanish families affected by cardioskeletal myopathy. A selective pattern of muscle involvement, which differed from that observed in myofibrillar myopathy resulting from mutations in the myotilin gene, was observed in each of the three families with novel mutations and each of three desminopathy patients with known desmin mutations. Prominent joint retractions at the ankles and characteristic nasal speech were observed early in the course of illness. These findings suggest that muscle imaging in combination with routine clinical and pathological examination may be helpful in distinguishing desminopathy from other forms of myofibrillar myopathy and ordering appropriate molecular investigations.
The effect of basal dietary supplemented with vegetable oils plus vitamin E (sunflower, soybean, ... more The effect of basal dietary supplemented with vegetable oils plus vitamin E (sunflower, soybean, linseed and a basal diet control), type of packaging (MAP or vacuum), addition of natural antioxidant (grape seed, rosemary) and storage time (0, 7, 14 and 21 days) on lipid oxidation, color stability, vitamin E content, and total aerobic bacterial counts in steaks of Longissimus thoracis was studied. The triple interaction diet × time × packaging affected oxidative stability, redness and yellowness of the meat. TBARS values did not increase with time in vacuum-packaged samples for all dietary treatments. However, samples from MAP and control showed the highest TBARS values after 21 days of storage (0.72 mg MDA/kg of meat, P<0.05). Both exogenous antioxidant extracts and MAP maintained low total aerobic counts in steaks until the 21st day. Calves should be fed a diet supplemented with L-VE, stored in MAP and treated with grape seed extract to extend the shelf life of their meat.
Background: Severe COVID-19 has been proposed to represent a form of viral sepsis. The prevalence... more Background: Severe COVID-19 has been proposed to represent a form of viral sepsis. The prevalence of sepsis due to this disease has not been evaluated. Some patients with severe COVID-19 present SARS-CoV-2 antigenemia. Whether antigenemia constitutes a factor associated to viral sepsis and fatal outcome in these patients is unknown. Methods: This study recruited 400 patients hospitalized with COVID-19. Prevalence of sepsis in the first 24 hours following hospitalization was determined using the SEPSIS-3 criteria. Prevalence and concentration of SARS-CoV-2 N-antigenemia was determined in plasma at hospitalization using the Panbio® COVID-19 Ag Rapid Test from Abbott and the COV-QUANTO® ELISA from AAZ. Findings: The prevalence of sepsis due to SARS-CoV-2 infection at hospitalization was of 54%, affecting mostly to the respiratory, renal and coagulation function. Antigenemia was present in the 53% of the patients, and constituted an independent risk factor of sepsis in the multivariate analysis (OR [CI95%], p): presence of N-antigenemia (Panbio®): (1·6 [1·1 - 2·5],0·023);concentration of N-antigen >575 pg/mL (COV-QUANTO®): (1·9 [1·0 - 3·5], 0·043). Antigenemia was associated with a reduction in the survival mean time of 9·42 and 9·33 days in the 90 first days following hospitalization, based on the PANBIO® and the COV-QUANTO® tests respectively. Interpretation: Half of the COVID-19 patients needing of hospitalization fulfill the criteria of sepsis. Antigenemia is strongly linked to the presence of this condition, and confers a higher risk of mortality. Monitoring the presence of antigenemia at the earlier moments of COVID-19 could help to identify those patients at risk of deterioration. Clinical Trial Registration Details: This was a sub-study of the CIBERES-UCI-COVID project, registered at Clinicaltrials.gov with the identification NCT04457505. Funding Information: Instituto de Salud Carlos III (COV20/00110;CP20/00041;FI20/00278), Fundació La Marató de TV3 (473/C/2021).Declaration of Interests: AT, JME, FB, RA and JFBM have a patent application on SARS-CoV-2 antigenemia. The remainder authors declare no conflicts of interest regarding this work.Ethics Approval Statement: Written or oral informed consent was obtained directly from all patients, or their legal representative, before enrolment. Scientific and ethical approval of the study protocol was obtained from the respective scientific committees for clinical research of the participant hospitals.
Archivos Latinoamericanos De Nutricion, Jun 1, 2000
The purpose of this study is to obtain a low cholesterol egg powder for the preparation of differ... more The purpose of this study is to obtain a low cholesterol egg powder for the preparation of different foods for persons whose egg consumption is restricted. Egg white and yolk mixtures prepared in different proportions were dehydrated; the following dried mixtures were obtained: A (1:1), B (2:1) and C (3:1) of egg white:yolk respectively. These mixtures were evaluated using the following parameters: proximal analysis, microbiological assay and protein quality evaluation. Physical characteristics of the powder and the sensorial tests of foods prepared with these mixtures were carried out. The fat and the cholesterol content in the mixture C were decreased by 40% and 20% respectively. The microbiological tests showed that the three mixtures were safe for human consumption. The PER of sample A (whole egg) was 3.65 and for the mixture C 3:1 egg white:yolk was 3.05. The PER of the 50:50 protein mixtures eggs white and yolk: with corn lime treated flour (HMN) were higher than that of the casein standard. The sensorial tests of the foods prepared with all the mixtures were acceptable.
Tuberculosis dynamic in Mexico and the world is forcing to readdress the procedures against this ... more Tuberculosis dynamic in Mexico and the world is forcing to readdress the procedures against this disease. In this sense the molecular diagnosis by the acid nucleic amplification from Mycobacteria by the polymerase chain reaction, is been pointing as a technique with certain success in countries with medium and high prevalence of the disease. So the main goal of this work is to evaluate utility of the diagnostic of tuberculosis by the PCR technique, amplifying the rDNA16s marker, from sputum samples from patients with suspicious of tuberculosis. The results show that from 107 clinical samples analyzed, 36 were positive to PCR and 37 were acid fast bacilli smear, the sensibility and specificity of the procedure were 80 and 90% respectively and the positive and negative predictive value was 80 and 88%, the average time for the development of the procedure was 16 hrs. Despite that this is a preliminary study, our results let us to claim the utility of this fast and confidence diagnostic procedure, as an important tool against TB.
Background: Skills in aseptic technique are essential competencies for preventing infection; yet,... more Background: Skills in aseptic technique are essential competencies for preventing infection; yet, aseptic technique is a difficult concept to master. During urinary catheter insertion, failure to properly adhere to strict aseptic technique has been linked to infections and sepsis. This pilot study used simulation to identify the most common breaches in aseptic technique during urinary catheter insertion. Methods: Baccalaureate nursing students with prior documentation of competency were recruited to demonstrate performance of urinary catheterization on a task trainer. The procedure was recorded, and breaches in technique were identified through review of the digital recordings. Results: Data were available for 13 participants. Participants ranged in age from 21 to 43 years (mean, 26.6). Ten participants (77%) were female and three (23%) were male. The participants' mean selfrating of confidence was 3.6 on a 5-point scale (range, 3-5), indicating some confidence in performing the skill. Examination of the video recorded data showed that 10 participants (77%) breached aseptic technique in at least one category and in some instances several categories. The most common breach was cleansing the meatus; seven participants (54%) performed it incorrectly. Conclusions: One-time competency validation using a simulator is not enough to ensure skill mastery or retention. Urinary catheter insertion is a high-risk skill and therefore should be afforded ample time to practice. Innovative approaches to ensure skill mastery, including urinary catheterization and other skills requiring sterile techniques, are needed.
Purpose. The purpose of this study was to evaluate medications that cancer survivors with oxalipl... more Purpose. The purpose of this study was to evaluate medications that cancer survivors with oxaliplatin-induced peripheral neuropathy take to control neuropathic symptom, and to explore self-management techniques used at home to provide temporary relief of painful neuropathy. This was a mixed methods, descriptive, cross-sectional study using self-reported data from colorectal cancer survivors previously treated with oxaliplatin. We analyzed demographic and medication data obtained from participants, along with written comments from an open-ended question regarding methods participants had tried to self-manage symptoms of neuropathy. Results. Twenty-nine percent of the sample reported taking some type of nutritional supplement with potential neuroprotective qualities. Opioids were being taken by 10% of the sample, and nonsteroidal anti-inflammatory and over-the-counter medications were taken by 15% of participants. Twelve percent of participants were taking antidepressants and 10% were...
The use of quartz tuning fork sensors as probes for scanning probe microscopy is growing in popul... more The use of quartz tuning fork sensors as probes for scanning probe microscopy is growing in popularity. Working in shear mode, some methods achieve a lateral resolution comparable with that obtained with standard cantilevered probes, but only in experiments conducted in air or vacuum. Here, we report a method to produce and use commercial AFM tips in electrically driven quartz tuning fork sensors operating in shear mode in a liquid environment. The process is based on attaching a standard AFM tip to the end of a fiber probe which has previously been sharpened. Only the end of the probe is immersed in the buffer solution during imaging. The lateral resolution achieved is about 6 times higher than that of the etched microfiber on its own.
This paper describes an experiment that studies the effect of basic haptic feedback in creating a... more This paper describes an experiment that studies the effect of basic haptic feedback in creating a sense of social interaction within a shared virtual environment (SVE). Although there have been a number of studies investigating the effect of haptic feedback on collaborative task performance, they do not address the effect it has in inducing social presence. The purpose of this experiment is to show that haptic feedback enhances the sense of social presence within a mediated environment. An experiment was carried out using a shared desktop based virtual environment where 20 remotely located couples who did not know one another had to solve a puzzle together. In 10 groups they had shared haptic communication through their hands, and in another group they did not. Hence the haptic feedback was not used for completing the task itself, but rather as a means of social interacting-communicating with the other participant. The results suggest that basic haptic feedback increases the sense of social presence within the shared VE.
Based on the structural style and physiographic criteria, the Central Andes of Peru can be divide... more Based on the structural style and physiographic criteria, the Central Andes of Peru can be divided into segments running parallel to the Pacific coast. The westernmost segment, the Coastal Belt, consists of a Late Jurassic-Cretaceous volcanic arc sequence that was accreted to the South American craton in Cretaceous times. The Mesozoic strata of the adjacent Western Cordillera represent an ENE-vergent fold-and-thrust belt that formed in Eocene times. Tight upright folds developed above a shallow detachment horizon in the West, while more open folds formed above a deeper detachment horizon towards the East and in the neighboring Central Highlands. A completely different style with steeply dipping reverse faults and open folds affecting the Neoproterozoic crystalline basement is typical for the Eastern Cordillera. The Subandean Zone is characterized by mainly NE-vergent imbricate thrusting which occurred in Neogene times. A quantitative estimate of the shortening of the orogen obtained from balanced cross-sections indicates a total shortening of 120-150 km (24%-27%). This shortening was coevel with the Neogene westward drift of South America, occurred at rates between 3 and 4.7 mm/year and was responsible for the high elevation of the Peruvian Andes.
This paper examines the effects of lender and borrower personal characteristics (perceived attrac... more This paper examines the effects of lender and borrower personal characteristics (perceived attractiveness, age and gender) on online peer-to-peer lending decisions. The extant research in finance, marketing, and psychology provides substantial support for the "beauty premium" effect, where ceteris paribus, more (as opposed to less) attractive individuals are favored (e.g., Ravina 2012). Our results qualify these findings, suggesting that (1) when perceived age is a clear signal of competence (college-age signals low competence, while middle age signals significant competence), attractiveness has no effect on loan success; (2) when lender and borrower are of the same gender, attractiveness may actually hurt one’s chances to secure a loan (we observe "beauty is beastly" effect), and (3) loan success is sensitive to the relative age and attractiveness of lenders and borrowers.
Thanks to Christopher James, Anthony Saunders, Allen Berger, Joel Houston, Lars Norden, Andy Nara... more Thanks to Christopher James, Anthony Saunders, Allen Berger, Joel Houston, Lars Norden, Andy Naranjo, Mahendrarajah Nimalendran, and seminar participants at the University of Florida, Financial Management Association and Eastern Finance Association for helpful ...
Quartz tuning fork devices are increasingly being used as nanosensors in Scanning Probe Microscop... more Quartz tuning fork devices are increasingly being used as nanosensors in Scanning Probe Microscopy. They offer some benefits with respect to standard microfabricated cantilevers in certain experimental setups including the study of biomolecules under physiological conditions. In this work, we compare three different working modes for imaging micropatterned antibodies with quartz tuning fork sensors: apart from the classical amplitude and frequency modulation strategies, for first time the jumping mode is implemented using tuning forks. Our results show that the molecules suffer less degradation when working in the jumping mode, due to the reduction of the interaction forces.
Angiotensin I converting enzyme (ACE) was studied in Vero cells, rabbit corneal fibroblasts, and ... more Angiotensin I converting enzyme (ACE) was studied in Vero cells, rabbit corneal fibroblasts, and rabbit corneal endothelial cells. The enzyme activity was determined by means of an assay employing hippuryl-glycyl-glycine as a substrate. The hippuric acid end product was separated from the substrate by reversed phase liquid chromatography and measured spectrophotometrically at 228 nm. The enzyme was further characterized by a captopril inhibition study. Significant ACE activity was found in rabbit corneal endothelial cells but not in other types of cells tested. This is the first report of the presence of this enzyme in a specific ocular cell type and suggests that angiotensin II may play a role in normal ocular physiology.
A 27-year-old woman of Moldavian origin presented at the age of 15 with progressive proximal limb... more A 27-year-old woman of Moldavian origin presented at the age of 15 with progressive proximal limb weakness and painful cramps in her calf muscles. Clinical examination revealed prominent muscle weakness in proximal muscles of the lower extremities and distal anterior compartment of legs, and mild weakness in shoulder girdle muscles. In addition, she had marked calf hypertrophy, muscle atrophy involving the anterior and posterior compartments of the thighs, and the distal anterior compartment of legs, as well as mild scapular winging and hyperlordosis. A muscle biopsy taken from the biceps brachii showed mild dystrophic changes, absent vacuoles, and abundant lobulated fibers. Immunofluorescence and Western blot assays demonstrated complete telethonin deficiency. Molecular analysis revealed a homozygous Trp25X mutation in the telethonin (TCAP) gene resulting in termination of transcription at an early point. Four families from Brazil with telethonin deficiency have previously been reported and classified as LGMD2G, but the actual frequency of this disease is unknown. With this current identification of a case outside the Brazilian population, telethonin mutation-associated LGMD should be considered worldwide.
Desminopathy represents a subgroup of myofibrillar myopathies caused by mutations in the desmin g... more Desminopathy represents a subgroup of myofibrillar myopathies caused by mutations in the desmin gene. Three novel disease-associated mutations in the desmin gene were identified in unrelated Spanish families affected by cardioskeletal myopathy. A selective pattern of muscle involvement, which differed from that observed in myofibrillar myopathy resulting from mutations in the myotilin gene, was observed in each of the three families with novel mutations and each of three desminopathy patients with known desmin mutations. Prominent joint retractions at the ankles and characteristic nasal speech were observed early in the course of illness. These findings suggest that muscle imaging in combination with routine clinical and pathological examination may be helpful in distinguishing desminopathy from other forms of myofibrillar myopathy and ordering appropriate molecular investigations.
The effect of basal dietary supplemented with vegetable oils plus vitamin E (sunflower, soybean, ... more The effect of basal dietary supplemented with vegetable oils plus vitamin E (sunflower, soybean, linseed and a basal diet control), type of packaging (MAP or vacuum), addition of natural antioxidant (grape seed, rosemary) and storage time (0, 7, 14 and 21 days) on lipid oxidation, color stability, vitamin E content, and total aerobic bacterial counts in steaks of Longissimus thoracis was studied. The triple interaction diet × time × packaging affected oxidative stability, redness and yellowness of the meat. TBARS values did not increase with time in vacuum-packaged samples for all dietary treatments. However, samples from MAP and control showed the highest TBARS values after 21 days of storage (0.72 mg MDA/kg of meat, P<0.05). Both exogenous antioxidant extracts and MAP maintained low total aerobic counts in steaks until the 21st day. Calves should be fed a diet supplemented with L-VE, stored in MAP and treated with grape seed extract to extend the shelf life of their meat.
Uploads
Papers by Laura Gonzalez