Papers by Rosane N M Guerra
Evidence-Based Complementary and Alternative Medicine, 2016
Attalea speciosasynOrbignya phalerataMart. (babassu) has been used in the treatment of inflammato... more Attalea speciosasynOrbignya phalerataMart. (babassu) has been used in the treatment of inflammatory and infectious diseases.Aim of the study. To investigate the antimicrobial and immunological activity of babassu mesocarp extract (EE).Material and Methods.Thein vitroantimicrobial activity was evaluated by disk diffusion assay and by determination of the minimum inhibitory concentration (MIC) toEscherichia coli,Pseudomonas aeruginosa,Enterococcus faecalis,Staphylococcus aureus,and methicillin-resistantStaphylococcus aureus(MRSA). The flavonoids and phenolic acids content were determined by chromatography. Thein vivoassays were performed in Swiss mice submitted to sepsis by cecal ligation and puncture (CLP). The mice received EE subcutaneously (125 or 250 mg/Kg), 6 hours after the CLP. The number of lymphoid cells was quantified and the cytokines production was determined by ELISA after 12 h.Results.EE was effective as antimicrobial toE. faecalis,S. aureus, and MRSA. EE is rich in phe...
In an effort to identify novel therapeutic alternatives for the treatment of malaria, the present... more In an effort to identify novel therapeutic alternatives for the treatment of malaria, the present study evaluated the antimalarial effect of the crude hydroalcoholic extract (HCE) from the leaves of Chenopodium ambrosioides L. For this purpose, the molecular affinity between the total proteins from erythrocytes infected with Plasmodium falciparum and HCE or chloroquine was evaluated by surface plasmon resonance (SPR). Subsequently, the plasmodicidal potential of HCE was assessed in a P. falciparum culture. Using BALB/c mice infected with Plasmodium berghei intraperitoneally (ip.), we evaluated the effects of ip. treatment, for three consecutive days (day 7, 8, and 9 after infection), with chloroquine (45 mg/kg) or HCE (5 mg/kg), considering the survival index and the parasitaemia. The groups were compared to an untreated control group that receives only PBS at the same periods. The results indicated that HCE could bind to the total proteins of infected erythrocytes and could inhibit the parasite growth in vitro (IC 50 = 25.4 g/mL). The in vivo therapeutic treatment with HCE increased the survival and decreased the parasitaemia in the infected animals. Therefore, the HCE treatment exhibited a significant antiplasmodial effect and may be considered as a potential candidate for the development of new antimalarial drugs.
Revista Brasileira De Farmacognosia, 2007
A atividade anti-Leishmania do extrato hidroalcoólico de Stachytarpheta cayennensis, espécie util... more A atividade anti-Leishmania do extrato hidroalcoólico de Stachytarpheta cayennensis, espécie utilizada popularmente no tratamento de lesões cutâneas causadas por Leishmania sp, foi testado em ensaios in vitro utilizando formas promastigotas de Leishmania braziliensis e L. amazonensis. O extrato hidroalcoólico foi preparado a partir das folhas secas e utilizado em culturas de L. amazonensis e L. braziliensis nas concentrações de 500 a 32,5 g/mL. Após 24 horas as formas promastigotas foram quantifi cadas para o cálculo da CI 50 . A citotoxicidade do extrato foi avaliada também em culturas de macrófagos peritoneais. O extrato apresentou efeito leishmanicida dose e espécie-dependente para promastigotas de Leishmania sendo mais efi caz para L. braziliensis. O extrato não apresentou efeito citotóxico quando utilizado nas culturas de macrófagos. Concluiuse que o extrato hidroalcoólico de S. cayennensis inibe formas promastigotas de Leishmania in vitro o que poderia justifi car, pelo menos parcialmente, o uso popular dessa espécie no tratamento de úlceras causadas por Leishmania.
Respiratory allergies are becoming increasingly frequent, especially based on studies of asthma a... more Respiratory allergies are becoming increasingly frequent, especially based on studies of asthma and rhinitis. It is estimated that 20-30% of the world's population is affected. Allergic reactions are caused by the production of IgE antibodies specific to inhaled allergens, such as fungi in the air. This study aimed to analyze the level of specific IgE against airborne fungi in patients with a clinical diagnosis of asthma and rhinitis/sinusitis. In total, 158 patients enrolled in the Program of Support for Asthmatic Patient, and 20 controls were studied. Clinical data from the period of 2007-2008 were surveyed using a protocol form. ELISAs were performed to quantify the levels of total and specific IgE. Of the 158 patients diagnosed with asthma, 71 had rhinitis and 32 had sinusitis. There was a predominance of females and residents of urban areas. The main symptoms reported were dyspnea, cough, wheezing and nasal obstruction. There was a statistically significant relationship between dyspnea and seropositivity for Fusarium (p = 0.01) and Penicillium (p = 0.005) and between cough and seropositivity for Aspergillus (p = 0.007). Anti-Penicillium (79.7%) and anti-Fusarium IgE (77.8%) were found to have the highest prevalence of seropositivity in individuals with asthma and rhinitis/sinusitis. Sensitivity to fungi was higher in symptomatic individuals. The identification of environmental fungi is essential for the diagnosis of respiratory allergy.
BMC Research Notes, 2016
The aim of this study was to evaluate the biochemical and immunological characteristics of saliva... more The aim of this study was to evaluate the biochemical and immunological characteristics of saliva from diabetic patients compared to non-diabetic adults. Eighty-eight diabetic adults and 39 non-diabetic adults (control) were included in the study. Glucose, urea, calcium, total protein and amylase were determined by a colorimetric method. The levels of secretory IgA and the IgA anti-Streptococcus mutans and anti-insulin IgA antibodies were measured by enzyme-linked immunosorbent assay (ELISA). Caries status was evaluated using the DMFT index. Glucose, urea, calcium, anti-S. mutans IgA, total IgA, and anti-insulin IgA were significantly higher in diabetic patients, whereas total protein and amylase levels were lower in these patients. There was no positive correlation between blood and salivary glucose levels in either group. Diabetic patients had a higher DMFT index. The present study showed for the first time that IgA levels in diabetic patients'saliva, shows correlation with systemic biochemical parameters. Thus the saliva is an useful tool to follow the systemic health status in these patients.
Periodontia, Dec 30, 2007
A insuficiência renal crônica (IRC) representa uma alteração estrutural renal que implica na redu... more A insuficiência renal crônica (IRC) representa uma alteração estrutural renal que implica na redução ou limitação da capacidade de filtração glomerular dos rins, causando a uremia que se caracteriza pelo acúmulo no sangue de substâncias que devem ser filtradas e excretadas pelos rins 15 .
Cadernos De Prospeccao, Apr 7, 2014
Periodontia, Sep 30, 2007
Page 1. 3 0 R. Periodontia - Setembro 2007 - Volume 17 - Número 03 INTRODUÇÃO A doença periodonta... more Page 1. 3 0 R. Periodontia - Setembro 2007 - Volume 17 - Número 03 INTRODUÇÃO A doença periodontal pode ser definida como uma infecção dos tecidos periodontais por microrganismos capazes de formar o biofilme dental ...
Revista De Ciencias Da Saude, Oct 24, 2012
Brazilian Journal of Medical and Biological Research
C57B1/6J mice received ovalbumin (Ova) orally, 20 mg/day, from day of parturition for 3, 5, 7, 10... more C57B1/6J mice received ovalbumin (Ova) orally, 20 mg/day, from day of parturition for 3, 5, 7, 10 or 15 days. Anti-Ova antibodies were titrated in plasma and milk by passive hemagglutination, and Ova-specific plaque-forming cells (PFC) were counted in the spleen and mesenteric lymph nodes. Anti-Ova antibodies in milk and antibody-secreting cells in mesenteric lymph nodes and spleen were detected on day 3, increased on day 5 and peaked on day 10. In contrast, anti-Ova antibodies in serum and PFC in spleen were low on day 7 and decreased on days 10 to 15. Although the oral administration of this antigen has been used to induce oral tolerance or secretory immune responses in the mouse, the present study demonstrates that the repeated ingestion of ovalbumin results in the development of circulating and secretory antibodies.
Journal of Immunological Methods, 1982
Transplantation of ascitic P-815 mastocytoma cells intradermally in the ears of syngeneic DBA/2J ... more Transplantation of ascitic P-815 mastocytoma cells intradermally in the ears of syngeneic DBA/2J mice allows a simple and precise quantitation of tumor growth by ear swelling. Tumor growth was retarded and then arrested in allogeneic DBA/1J mice, whereas syngeneic hosts died as a result of tumor dissemination in draining lymph nodes, liver and spleen.
Brazilian Journal of Medical and Biological Research
We studied the effects of chronic voluntary ingestion of "Tiquira" (50%)--an alcoholic ... more We studied the effects of chronic voluntary ingestion of "Tiquira" (50%)--an alcoholic beverage prepared from fermented manioc, widely consumed in Maranhão,--on the natural immunological activity of young adult (2-3 months old) C57B1/6J mice (16-17 g) by evaluating the number of plaque-forming cells (PFCs) in the spleen and by titrating serum antibodies by ELISA. Voluntary ingestion of "Tiquira" for 30 days decreased immunoglobulin secretion in serum (Control: 1600 +/- 30 vs Experimental: 193 +/- 20), caused an impressive reduction in the total number of PFCs in the spleen (Control: 482 +/- 22 vs Experimental: 58 +/- 3) and increased the proportion of self-reacting antibody molecules in serum (Control: 119 +/- 16 vs Experimental: 800 +/- 20) and self-reactive PFCs to mouse red blood cells (MRBC) in the spleen (Control: 183 +/- 14 vs Experimental: 272 +/- 16; N = 10 animals per group). These preliminary results suggest that the voluntary ingestion of "Tiquira...
Brazilian Oral Research, 2011
International Journal of Pediatric Otorhinolaryngology, 2013
Revista Brasileira de Farmacognosia, 2011
... SB Barroqueiro; Fernanda SB Barroqueiro; Mayara T. Pinheiro; Márcia CG Maciel; Priscila S. Ba... more ... SB Barroqueiro; Fernanda SB Barroqueiro; Mayara T. Pinheiro; Márcia CG Maciel; Priscila S. Barcellos; Lucilene A. Silva; Adelson S. Lopes; Flávia RF Nascimento ... for use by the Brazilian people as food supplement, since it is rich in carbohydrates and mineral salts (Gaitan et al ...
Revista Brasileira de Farmacognosia, 2010
ABSTRACT It was investigated the effect of hydroalcoholic extract (HEP) of propolis from Scaptotr... more ABSTRACT It was investigated the effect of hydroalcoholic extract (HEP) of propolis from Scaptotrigona aff. postica on the solid Ehrlich tumor, on the tumor-bearing mice lymphoid organs and on the nitric oxide (NO) production. Swiss mice were divided in 4 groups: control, HEP 0.5; HEP 5 and HEP 50 that was treated by intraperitoneal route with a single dose of saline solution (NaCl 0.9%) or 0.5 or 5 or 50 mg of HEP/kg body weight, respectively. After 48 h of treatment, the animals were inoculated with 105 tumor cells in their footpad. The results showed that the treatment with HEP in the doses of 5 and 50 mg/kg inhibited the development of the tumor from the 6th day post inoculums when compared to the control and to the HEP 0.5 groups. Besides, there was an increase of spleen and bone marrow cell number in HEP 0.5 and HEP 5 as compared to the control. Concanavalin A (ConA)-stimulated NO production was decreased in all HEP-treated groups when compared to the control. In conclusion, the treatment with HEP had an anti-tumor effect what may be related to its chemical composition and to the inhibition of NO production.
Revista Brasileira de Farmacognosia, 2012
... Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propoli... more ... Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review. Marcio AR Araujo I , * ; Silvana A. Libério I ; Rosane NM Guerra I ; Maria Nilce S. Ribeiro II ; Flávia RF Nascimento I. ... Menezes H, Alvarez JM, Almeida EC 1999. ...
Journal of Asthma, 2014
Respiratory allergies are becoming increasingly frequent, especially based on studies of asthma a... more Respiratory allergies are becoming increasingly frequent, especially based on studies of asthma and rhinitis. It is estimated that 20-30% of the world's population is affected. Allergic reactions are caused by the production of IgE antibodies specific to inhaled allergens, such as fungi in the air. This study aimed to analyze the level of specific IgE against airborne fungi in patients with a clinical diagnosis of asthma and rhinitis/sinusitis. In total, 158 patients enrolled in the Program of Support for Asthmatic Patient, and 20 controls were studied. Clinical data from the period of 2007-2008 were surveyed using a protocol form. ELISAs were performed to quantify the levels of total and specific IgE. Of the 158 patients diagnosed with asthma, 71 had rhinitis and 32 had sinusitis. There was a predominance of females and residents of urban areas. The main symptoms reported were dyspnea, cough, wheezing and nasal obstruction. There was a statistically significant relationship between dyspnea and seropositivity for Fusarium (p = 0.01) and Penicillium (p = 0.005) and between cough and seropositivity for Aspergillus (p = 0.007). Anti-Penicillium (79.7%) and anti-Fusarium IgE (77.8%) were found to have the highest prevalence of seropositivity in individuals with asthma and rhinitis/sinusitis. Sensitivity to fungi was higher in symptomatic individuals. The identification of environmental fungi is essential for the diagnosis of respiratory allergy.
Journal of Oral Pathology & Medicine, 2012
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Papers by Rosane N M Guerra