Papers by Silvana Libério
Rev Bras Odontol, Jun 1, 1982
Pediatric dentistry
The purpose of this study was to estimate the association between low birth weight and its 2 comp... more The purpose of this study was to estimate the association between low birth weight and its 2 components, intrauterine growth restriction (IUGR) and preterm birth, with developmental defects of dental enamel (DDE). A retrospective cohort study was conducted on 205 12- to 60-month-olds born in the municipality of São Luís, Maranhão, Brazil. Clinical examination and interviews with the responsible persons were performed, and the medical records were analyzed. The modified DDE index was used for the diagnosis and classification of enamel defects. Poisson regression analysis was performed, and relative risk (RR) ratios (95% confidence interval, CI) were used for the estimation of associations. Preterm birth was not associated with DDE in nonadjusted or adjusted analysis. In the crude models and after adjusting for confounding factors, IUGR (RR=5.19; CI=2.42-11.13) and low birth weight (RR=3.44; CI=1.72-7.37) continued to be risk factors for the occurrence of DDE. Low birth weight infants...
Journal of Clinical Periodontology, 2014
Aim: To investigate the association between periodontal status and serum biomarkers levels in hae... more Aim: To investigate the association between periodontal status and serum biomarkers levels in haemodialysis patients. Methods: This cross-sectional study included 96 haemodialysis patients. The periodontal evaluation was realized using clinical attachment level (CAL), probing depth (PD), gingival bleeding index (GBI), visible plaque index (VPI) and gingival index (GI). Biochemical and haematological dataserum albumin, phosphorus, creatinine, transferrin, ferritin, iron, alkaline phosphatase, calcium, potassium and haemoglobinwere collected from the medical records. The subject was diagnosed with periodontitis if he/she had at least two inter-proximal sites in different teeth with CAL ≥4 mm and/or at least two inter-proximal sites in separate teeth with PD ≥5 mm. Results: The study population consisted of 45 men and 51 women, with mean time under haemodialysis of 45.6 AE 33.1 months. Periodontitis was observed in 59.4% of the subjects. The periodontitis group had albumin (p = 0.021) and phosphorus (p = 0.024) serum levels lower than the no periodontitis group. Thus, there was a positive association of periodontitis with hypoalbuminaemia (OR = 9.10, p = 0.006) and a negative association with hyperphosphataemia (OR = 0.21, p = 0.010). Conclusions: These findings suggest that periodontitis is associated with albumin and phosphorus serum levels in haemodialysis patients.
Revista Brasileira de Farmacognosia, 2010
Neste trabalho foi investigado o efeito do extrato hidroalcoólico de própolis (EHP) de Scaptotrig... more Neste trabalho foi investigado o efeito do extrato hidroalcoólico de própolis (EHP) de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich na forma sólida, sobre a celularidade dos órgãos linfóides dos animais portadores de tumor, bem como, sobre a produção de óxido nítrico (NO) pelos macrófagos destes animais. Camundongos Swiss foram divididos em quatro grupos: controle, EHP 0,5; EHP 5 e EHP 50, os quais foram tratados por via intraperitoneal com dose única de solução salina (NaCl 0,9%); 0,5; 5 ou 50 mg de EHP/kg de animal, respectivamente. Depois de 48 h do tratamento, os animais foram inoculados com 10 5 células do tumor de Ehrlich nas patas. Os resultados mostraram que o tratamento com EHP nas doses de 5 e 50 mg/kg inibiu de forma significativa o desenvolvimento do tumor a partir do 6º dia pós-inóculo quando comparado ao controle e ao EHP 0,5. Além disso, houve aumento significativo da celularidade do baço e da medula óssea nos grupos EHP 0,5 e EHP 5 em relação ao controle. A produção de NO estimulada com concanavalina A (ConA) apresentou uma significante diminuição nos grupos tratados com EHP em relação ao controle. Pode-se concluir que o tratamento com EHP apresentou efeito antitumoral quando administrado nas doses de 5 e 50 mg/kg, o que pode estar relacionado com a sua composição química e com a inibição da produção de NO. Unitermos: própolis, Scaptotrigona aff. postica, tumor de Ehrlich, abelhas sem ferrão, Meliponinae. ABSTRACT: "Effect of propolis of Scaptotrigona aff. postica on the development of the tumor of Ehrlich in mice". It was investigated the effect of hydroalcoholic extract (HEP) of propolis from Scaptotrigona aff. postica on the solid Ehrlich tumor, on the tumor-bearing mice lymphoid organs and on the nitric oxide (NO) production. Swiss mice were divided in 4 groups: control, HEP 0.5; HEP 5 and HEP 50 that was treated by intraperitoneal route with a single dose of saline solution (NaCl 0.9%) or 0.5 or 5 or 50 mg of HEP/kg body weight, respectively. After 48 h of treatment, the animals were inoculated with 10 5 tumor cells in their footpad. The results showed that the treatment with HEP in the doses of 5 and 50 mg/kg inhibited the development of the tumor from the 6 th day post inoculums when compared to the control and to the HEP 0.5 groups. Besides, there was an increase of spleen and bone marrow cell number in HEP 0.5 and HEP 5 as compared to the control. Concanavalin A (ConA)-stimulated NO production was decreased in all HEP-treated groups when compared to the control. In conclusion, the treatment with HEP had an anti-tumor effect what may be related to its chemical composition and to the inhibition of NO production.
Journal of Oral Pathology & Medicine, 2012
This study evaluated the salivary biochemical and immunological status of children with cancer un... more This study evaluated the salivary biochemical and immunological status of children with cancer undergoing to antineoplasic treatment in an attempt to identify alternatives for a less invasive and less painful monitoring of these patients. MATERIALS AND METHODS: Unstimulated whole saliva samples were obtained from 115 children without cancer (control) and 32 children with cancer (CA). Children with cancer were also evaluated after antineoplasic treatment (CAT, n = 17). The salivary concentrations of glucose, triglycerides, total cholesterol, alkaline phosphatase, gamma-glutamyltransferase (GGT), urea, insulin, thyroid-stimulating hormone (TSH), triiodothyronine (T3), levothyroxine (T4), and immunoglobulin A (IgA) were determined. RESULTS: Acute lymphocytic leukemia, acute myeloid leukemia, and Hodgkin's lymphoma were the most frequent cancers, although cases of non-Hodgkin's lymphoma, medulloblastoma, ependymoma, osteosarcoma, nephroblastoma, Ewing's sarcoma, and endodermal sinus tumor were also observed. The salivary concentration of cholesterol, triglycerides, or GGT did not differ between groups. Instead, the concentrations of alkaline phosphatase and T4 were higher in patients with cancer, irrespective of treatment. TSH levels were higher in the CA group and urea concentration was lower in the CAT group. T3 was undetectable in all groups. Antineoplasic treatment increased the glucose level and decreased the insulin concentration. Salivary concentration of total IgA was lower in children with cancer, irrespective of treatment. CONCLUSIONS: Cancer and antineoplasic treatment affected biochemical and immunological parameters in the saliva of children, shedding new light on the potential usefulness of saliva for monitoring children with cancer, especially to patients undergoing immunosuppressive therapy.
Natural product research, 2011
The propolis of Scaptotrigona aff. postica is popularly used in Maranhão State, Brazil, for treat... more The propolis of Scaptotrigona aff. postica is popularly used in Maranhão State, Brazil, for treating wounds and respiratory illnesses. Nevertheless, little is known about the chemical composition of this propolis and the adverse effects of its use. Hence, this study is a pharmacognostic characterisation of the propolis hydroalcoholic extract (PHE) from S. aff. postica. The methodology consisted of an evaluation of the sensory and chemical parameters. Chemical analysis of PHE indicated high concentrations of phenolic and triterpens substances, and the absence of steroids. Additionally, we evaluated the acute toxicity of propolis using 48 Swiss male and female mice. The animals received single doses of PHE (1000, 2000 or 4000 mg kg⁻¹) orally and were observed for 14 days. After this period, the mice were sacrificed and the blood was used for biochemical and haematological evaluation. PHE did not induce any death, and the acute treatment significantly reduced serum concentrations of al...
RGO
Objective: To determine the presence of oral mucosa alterations in children from 3 to 12 year of ... more Objective: To determine the presence of oral mucosa alterations in children from 3 to 12 year of age, hospitalized in the period from January to May 2004. Methods: A clinical evaluation was made of the oral mucosa alterations in 165 hospitalized children from 3 to 12 of age, in ...
Purpose: To record the prevalence of congenital and developmental oral abnormalities in infants a... more Purpose: To record the prevalence of congenital and developmental oral abnormalities in infants aged 0 to 6 months. Methods: The sample of this descriptive study consisted of 621 infants aged 0 to 6 months, who attended the Milk Bank of the Hospital Universitario Maternal Infantil in Sao Luis, MA, Brazil, from January 2001 to June 2004. The clinical exam was accomplished through visual inspection, using natural light and wooden spatula. Data recorded were congenital and developmental oral abnormalities and their anatomic location, as well as the subject sex and age. Results: From the total of 621 subjects (310 males and 311 females), 45 (7.24 %) had some oral alteration, particularly in the maxilla. The inclusion cyst was the most frequent oral alteration (6.28 % of the subjects). The age group of infants from 0 to 03 months showed the largest number of oral abnormalities. Conclusion: Congenital and developmental oral abnormalities are not rare occurrences in infants aged from 0 to ...
LETÍCIA MACHADO GONÇALVES, JOSÉ RIBAMAR SABINO BEZERRA-JÚNIOR, MANUEL ANTONIO GORDÓN-NÚÑEZ, SILVA... more LETÍCIA MACHADO GONÇALVES, JOSÉ RIBAMAR SABINO BEZERRA-JÚNIOR, MANUEL ANTONIO GORDÓN-NÚÑEZ, SILVANA AMADO LIBÉRIO, ADRIANA DE FÁTIMA VASCONCELOS PEREIRA & MARIA CARMEN FONTOURA NOGUEIRA DA CRUZ Department of Dentistry, School of Dentistry, Federal University of Maranhão, São Luı́s, MA, Brazil, Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil, Department of Dentistry I, School of Dentistry, Federal University of Maranhão, São Luı́s, MA, Brazil, and Department of Dentistry II, School of Dentistry, Federal University of Maranhão, São Luı́s, MA, Brazil
Revista da Associacao Medica Brasileira, 2005
OBJECTIVES AND METHODS Thirty patients undergoing hemodialysis were evaluated for their periodont... more OBJECTIVES AND METHODS Thirty patients undergoing hemodialysis were evaluated for their periodontal condition through plaque, calculus and gingival indexes; PSR and IgA present in the saliva were also appraised in order to determine the periodontal condition of patients presenting chronic kidney disease. RESULTS Results obtained demonstrated that in the studied group the plaque, calculus and gingival indexes were high. With regard to these patients' requirement to undergo periodontal treatment, most patients submitted to hemodialysis needed better oral hygiene, in addition to supra and sub gingival scraping and topic application of fluor (code 2). As to the IgA amount present in the saliva, only three patients showed a low density. CONCLUSIONS Patients presenting chronic kidney disease disclosed a tendency for greater bacterial plaque concentration, high formation of dental calculus suggesting the need for periodontal treatment comprising supra and sub gingival scraping.
Journal of Biosciences and Medicines, 2016
Anacardium occidentale L., Anacardiaceae, is frequently used to treat infections. We evaluated th... more Anacardium occidentale L., Anacardiaceae, is frequently used to treat infections. We evaluated the antimicrobial and antioxidant activity of extracts obtained from aerial parts of the plant in comparison with the extract prepared with the flowers. Ethanol extracts of the leaves (EEL), stem bark (EEB), and flowers (EEF) were prepared separately. The antimicrobial activity was evaluated by agar diffusion and broth dilution methods. The minimum bactericidal and fungicidal concentrations were determined against: Streptococcus mutans, Lactobacillus acidophilus, Staphylococcus aureus, MRSA, Enterococcus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Klebsiella pneumoniae, Helicobacter pylori, Salmonella choleraesuis, Candida albicans, and Candida tropicalis. The antioxidant activity of the extracts was evaluated based on their scavenger capacity of 2,2-diphenyl-1-picrylhydrazyle. The phytochemical profile was analyzed by colorimetric methods and by HPLC with UV detection. All extracts exhibited antioxidant and antimicrobial activity. Although, the EEF was the most effective since it inhibited the growth of all 14 microorganisms tested. Anacardic and galic acids were identified in all extracts as well phenolic compounds, triterpenes, flavones and xanthones. In conclusion, the extracts obtained from the aerial parts of A. occidentale, mainly the extract of flowers that was the most effective, are rich in bioactive metabolites that exert a potent antioxidant and antimicrobial effect. Taken together, the results indicate an important biotechnological potential of A. occidentale as a
Objective: To determine the presence of oral mucosa alterations in children from 3 to 12 year of ... more Objective: To determine the presence of oral mucosa alterations in children from 3 to 12 year of age, hospitalized in the period from January to May 2004. Methods: A clinical evaluation was made of the oral mucosa alterations in 165 hospitalized children from 3 to 12 of age, in the period from January to May 2004, of both genders, interned in the three wings of the mother and child hospital “Hospital Universitario Materno Infantil” of the Federal University of Maranhao. Results: The most frequent alterations were encrusted tongue (61.82%), pseudomembranous candidiasis (5.45%) and recurrent herpetic infection (3.64%), no difference being found with regard to their presence in the age groups (3 to 6 years of age and 7 to 12 years of age), however, boys were more affected (85.90%) than girls (71.26%). Conclusion: Of the 165 children examined, 78.18% exhibited alterations and in certain cases, more than one alteration was observed at the time of the exam. Indexing terms: mouth mucosa; c...
Brazilian oral research, Jan 5, 2017
The aim of this study was to detect possible associations between respiratory pathogens from trac... more The aim of this study was to detect possible associations between respiratory pathogens from tracheal aspirate and oral biofilm samples in intubated patients in an intensive care unit (ICU), and to identify the most common respiratory pathogens in oral biofilm, particularly in patients that developed ventilator-associated pneumonia (VAP). Two oral biofilm samples were collected from the tongue of intubated patients (at admission and after 48 hours) and analyzed by culture with the Antibiotic Sensitivity Test. The results from the tongue biofilm samples were compared with the tracheal secretions samples. A total of 59.37% of patients exhibited the same species of pathogens in their tracheal aspirate and oral biofilm, of which 8 (42.1%) developed VAP, 10 (52.63%) did not develop pneumonia and one (5.26%) had aspiration pneumonia. There was a statistically significant association between presence of microorganisms in the tracheal and mouth samples for the following pathogens: Klebsiell...
International Journal of Pediatric Otorhinolaryngology, 2013
Cancer shows a high incidence and continues to be one of the leading causes of death in the world... more Cancer shows a high incidence and continues to be one of the leading causes of death in the world [1]. In childhood cancer is emerging as a major cause of death in Asia, Central and South America, Northwest Africa, and the Middle East, where fewer children are now dying from preventable infectious diseases [2-5]. Acute lymphoid leukemia (ALL) is the most common type of pediatric cancer [4,6], accounting for nearly 25-35% of all pediatric cancers [1,7,8]. Among the treatment modalities for ALL, chemotherapy (Ct) and bone marrow transplantation (BMT) are the most frequent [9,10]. However, the disease and the antineoplastic treatment for leukemia (ATL) have various side effects [11-13]. In the oral cavity, these manifestations include mucositis, xerostomia, dental caries, gingival bleeding, candidiasis, labial herpes, stomatitis aphthosa, angular cheilitis and other [14-24]. Mucosistis, xerostomia, dental caries and gingivitis are some of the most frequent [14,17,18,25]. However, the documentation of the ATL's side effects in oral health is specially based on case reports [
Journal of Ethnopharmacology, 2014
Ethnopharmacological surveys show that several plant species are used empirically by the populati... more Ethnopharmacological surveys show that several plant species are used empirically by the population, in oral diseases. However, it is necessary to check the properties of these plant species. To evaluate in vitro antimicrobial activity against Streptococcus mutans from plant species selected in a previous ethnopharmacology study. An ethnopharmacological survey was conducted with users of a dental clinic school services, located in Sao Luis, Maranhão, Brazil, aiming to identify plant species used in oral diseases treatment. From the ethnopharmacological survey, species were selected for in vitro antimicrobial activity evaluation against Streptococcus mutans, by agar diffusion method and determination of Minimum Inhibitory Concentration (MIC). Two hundred and seventy one people participated in the research: 55.7% reported the use of plants for medicinal purposes, 29.5% of which have knowledge and/or use plants for some type of oral disease. Thirty four species belonging to 24 (twenty four) botanical families were reported, being Aloe vera L., Anacardium occidentale L., Schinus terebinthifolius Raddi, Chenopodium ambrosioides L. and Punica granatum L. the most cited. The most commonly reported indications were healing after tooth extraction, followed by toothache, inflammation and bleeding gums., The determination of Minimum Inhibitory Concentration (MIC) demonstrated that Punica granatum L., Psidium guajava L. and Schinus terebinthifolius Raddi showed similar activity to 0.12% chlorhexidine, used as positive control. That result is important to follow up the study of these species in the search for new anticariogenic agents originated by plants.
International Journal of Paediatric Dentistry, 2010
Background. Juvenile dermatomyositis (JDM) is an idiopathic inflammatory myopathy of childhood an... more Background. Juvenile dermatomyositis (JDM) is an idiopathic inflammatory myopathy of childhood and adolescence, characterized by symmetrical weakness of proximal muscles and classical cutaneous features. Literature reports rarely describe or focus on oral lesions that are associated with this disease. Case report. This case describes a 4-year-old girl in whom the oral lesions were the initial manifestations of JDM. Physical examination revealed characteristic skin manifestations, proximal muscle weakness, extensive calcinosis, necrotic ulceration, complicated erysipelas, and diffuse alopecia. The diagnosis was established based on the clinical, histological, electroneuromyography, and biochemical findings. Conclusion. Recognition of gingival telangiectases as an important diagnostic marker of JDM leads us to suggest that identifying oral manifestations, which may be carried out by a paediatric dentist, contributes in establishing an early diagnosis and an immediate treatment of this condition.
Revista Brasileira de Farmacognosia, 2012
Many biological properties have been attributed to various types of propolis, including anti-infl... more Many biological properties have been attributed to various types of propolis, including anti-inflammatory, antimicrobial, antioxidant, antitumor, wound healing, and immunomodulatory activities. This article reviewed studies published that investigated the anti-inflammatory activity of propolis of different origins and/ or its isolated components, focusing on the mechanisms of action underlying this activity and also addressing some aspects of immunomodulatory effects. The search was performed of the following databases: PubMed, Science Direct, HighWire Press, Scielo, Google Academics, Research Gate and ISI Web of Knowledgement. The anti-inflammatory activity was associated with propolis or compounds such as polyphenols (flavonoids, phenolic acids and their esters), terpenoids, steroids and amino acids. CAPE is the most studied compounds. The main mechanisms underlying the anti-inflammatory activity of propolis included the inhibition of cyclooxygenase and consequent inhibition of prostaglandin biosynthesis, free radical scavenging, inhibition of nitric oxide synthesis, reduction in the concentration of inflammatory cytokines and immunosuppressive activity. Propolis was found to exert an antiinflammatory activity in vivo and in vitro models of acute and chronic inflammation and others studies, indicating its promising potential as anti-inflammatory agent of natural origin and as a source of chemical compounds for the development of new drugs.
Journal of Ethnopharmacology, 2009
Propolis is a resinous substance made by bees. It possesses many biological activities, and many ... more Propolis is a resinous substance made by bees. It possesses many biological activities, and many studies have reported its potential application in the control of dental caries. However, variability in the chemical composition of propolis is a potential problem in its quality control, especially since propolis has already been incorporated into products for oral use. Therefore, a critical analysis of the available data on propolis is warranted. The present review discusses the in vitro and in vivo studies published in the period between 1978 and 2008 regarding the effects of propolis on Streptococcus mutans growth, bacterial adherence, glucosyltransferase activity, and caries indicators. Several investigations carried out with crude propolis extracts, isolated fractions, and purified compounds showed reductions in Streptococcus mutans counts and interference with their adhesion capacity and glucosyltransferase activity, which are considered major properties in the establishment of the cariogenic process. Data from in vivo studies have demonstrated reductions in Streptococcus mutans counts in saliva, the plaque index, and insoluble polysaccharide formation. These findings indicate that propolis and/or its compounds are promising cariostatic agents. However, the variation in the chemical composition of propolis due to its geographical distribution is a significant drawback to its routine clinical use. Thus, further studies are needed to establish the quality and safety control criteria for propolis in order for it to be used in accordance with its proposed activity.
Periodontia, 2007
Resumo: OBJETIVOS E MÉTODOS: Objetivou-se estudar a condição periodontal e os níveis de Imunoglob... more Resumo: OBJETIVOS E MÉTODOS: Objetivou-se estudar a condição periodontal e os níveis de Imunoglobulina A (IgA) salivar em pacientes em hemodiálise. Foi feita a análise da condição periodontal de 60 pacientes, sendo 30 em hemodiálise e 30 sistemicamente ...
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Papers by Silvana Libério