Background: Citrullus colocynthis Schrad, also known as colocynth, is a very common fruit in trad... more Background: Citrullus colocynthis Schrad, also known as colocynth, is a very common fruit in traditional medicine; it is recognized by different pharmacologic activities in traditional Algerian medicine (i.e., purgative, anti-in ammatory, antidiabetic, analgesic, and antiepileptic. Some of these activities were con rmed in modern phytotherapy (Shaheen, et al., 2014). Several scienti c studies carried out on crude extracts of pulp and seed of colocynth have demonstrated the
Postprandial hyperglycemia plays an important role in the development of type 2 diabetes and has ... more Postprandial hyperglycemia plays an important role in the development of type 2 diabetes and has been proposed as an independent risk factor for cardiovascular diseases. Herbal medicine has been used for many years by different cultures around the world for the treatment of diabetes. Punica granatum is one of the traditional remedies used for that reason. In this study, we investigated the effects of organic extracts (ethyl acetate and n-butanol) from Punica granatum peel on postprandial hyperglycemia in vivo caused by glucose, sucrose, maltose, or starch loading. Oral administration of Punica granatum peel extracts (400 mg/kg b.w.) markedly lowered plasma glucose levels in fasted normal rats. We examined in vitro whether the organic fractions of Punica granatum peel have an antidiabetic effect through α-amylase inhibitory action. Ethyl acetate and n-butanol fractions of aqueous/acetone crude extracts displayed a potent inhibitory effect on α-amylase activity (IC50 = 0.85 and 2.13 g...
Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus... more Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus. Intra peritoneal admin-istration of the aqueous extract of the seed (1.25 g/kg body weight) to streptozotocin-induced diabetic rats produced a significant reduction of blood sugar level in the long term, while the same extract produced no alteration of glycaemia in normal rats in the short term. The aqueous extract of this plant seems to have maximal adverse effect and high LD100 value.
Introduction: Citrullus colocynthis Schrad, conjointly referred to as colocynth, could be a quite... more Introduction: Citrullus colocynthis Schrad, conjointly referred to as colocynth, could be a quite common fruit in ancient drugs; it's recognized by totally different medical specialty activities in ancient Algerian medicine (i.e., purgative, medicinal drug, medicament, analgesic, and medicine. a number of these activities were confirmed in trendy herbal therapy many scientific studies applied on crude extracts of pulp and seed of colocynth have incontestable the antimitotic impact medicamen antiparasitic larvicidal moreover as a preventive impact against fatness. However, the bioactive chemicals compounds accountable of these activities don't seem to be isolated and known nonetheless. This is perennial herbs sometimes trailing, usually found wild within the sandy lands of North West, the Punjab, Sind, and Central and southern Asian nation, and coromandal coast. conjointly found autochthonal in peninsula, West Asia, and Tropical continent and within the Mediterranean region. ...
This study was undertaken to evaluate the correlation between total phenolic and flavonoid conten... more This study was undertaken to evaluate the correlation between total phenolic and flavonoid contents and antioxidant activity of various extracts of Punica granatum peel using ferric reducing antioxidant power (FRAP) and radical scavenging methods (DPPH). Preliminary phytochemical screening shows that the major constituents of total extracts are tannins and flavonoids. Phenolic and flavonoid contents were determined using spectrophotometric methods of hydromethanolic and aqueous-acetone crude extracts and their fractions, showing a high content in ethyl acetate fractions of each extract (597.08 ± 3.9 and 602.8 ± 0.0 mg GAE/gE respectively) followed by n-butanol fractions of each extract (413.6 ± 1.48 and 417.56 ± 5.3 mg EQC/gE respectively). Crude extracts showed the lower contents than the ethyl acetate and n-butanol fractions. FRAP method revealed that the ethyl acetate fraction from hydromethanolic extract showed the highest power to reduce iron followed by n-butanol fraction from...
The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following ph... more The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following physiological and immunological changes: hyperglycemia and hypercholesterolemia involving mainly the free fraction of cholesterol, with an elevation of high-density-lipoprotein levels and a decrease in B and T splenic lymphocyte proliferation in the presence of different mitogens PHA-P, Con A and LPS. These results demonstrate the important modification that could be induced in sand rat by the standard laboratory diet as compared with natural diet, and thus the sand rat (P. obesus) appears to be an interesting model for studies on experimental diabetes mellitus.
1 Laboratoire d'antibiotiques et antifongiques : physico-chimie, synthèse et activités biolog... more 1 Laboratoire d'antibiotiques et antifongiques : physico-chimie, synthèse et activités biologiques, Département de biologie moléculaire et cellulaire, Faculté des Sciences de la nature et de la vie, de la terre et de l'univers, BP119, Université Abou bekr Belkaïd, Tlemcen 13000, Algérie. 2 Laboratoire de Produits Naturels, département de biologie moléculaire et cellulaire, Faculté des Sciences de la nature et de la vie, de la terre et de l'univers, BP119, Université Abou bekr Belkaïd, Tlemcen 13000, Algérie. ______________________________________________________________________________ ABSTRACT This study was designed to evaluate the antidiabetic effect of hydroalcoholic extract from Punica granatum peels in normal and streptozotocin-induced diabetic rats and its potent α-amylase inhibitory. Antidiabetic effect of this extract was investigated after oral administration at dose of 400 mg/kg b.w. In normal fasted rats, the test drug extract showed no hypoglycaemic effect b...
ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology in... more ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology industries both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites which are illustrated in many areas and even in therapy. We investigated the antimicrobial effects ofBerberis vulgaris, a plant used in Algeria, and particularly in Tlemcen region. The qualitative phytochemical examination performed onBerberis vulgaris root bark showed the presence of alkaloids, tannins, sterols, triterpenes and reducing compounds in large quantities, and it also revealed lower amounts of coumarins, terpenoids, saponins and mucilage. The detection of these chemical classes is followed by extraction using solvents with increasing polarity and extraction of total alkaloids. Then, the different extracts were tested for their antimicrobial potency on different reference strains of bacteria and yeasts, using the agar diffusion technique and the determination of minimum inhibitory concentration MIC. The methanolic macerated extract had the best antibacterial activity with inhibition diameters from 12.5 to 21 mm for some strains. Also,extracts of Berberis vulgarisand more specifically alkaloids showed a strong antifungal activity onCandida albicans yeasts (diameters inhibition 19 to 29 mm)
ABSTRACT Pharmacognosie Évaluation de l'activité antioxydante de Berberis vulgaris L.... more ABSTRACT Pharmacognosie Évaluation de l'activité antioxydante de Berberis vulgaris L. Résumé : Les substances naturelles issues des végétaux présen-tent des intérêts multiples mis à profit dans la biotechnologie tant dans l'industrie alimentaire, cosmétique que pharmaceu-tique. Parmi ces composés, on retrouve une grande partie des métabolites secondaires qui se sont illustrés dans beaucoup de domaines et même en thérapie. Dans ce contexte, nous avons recherché l'activité antioxydante de Berberis vulgaris, une plante utilisée en Algérie et plus particulièrement dans la région de Tlemcen. Après préparation des extraits à partir des écorces de racines de Berberis vulgaris, les alcaloïdes totaux ont été analysés par chromatographie sur couche mince, révélant la présence de six taches. Aussi, une analyse quantitative des polyphénols et des flavonoïdes de l'extrait hydrométhanolique a été réalisée, montrant une teneur de 10,48 mg GAE/g en polyphénols totaux et 2,05 mg CEQ/g en flavonoïdes. L'activité antioxydante des extraits hydrométhanolique et alcaloïdes totaux a été évaluée par deux méthodes : la réduction du fer et le piégeage du radical libre DPPH. Les résultats obtenus ont montré une activité élevée de l'extrait hydrométhanolique par rapport aux alcaloïdes totaux, avec une CI 50 de 1,40 mg/ml. Mots clés : Berberis vulgaris – Extrait hydrométhanolique – Alcaloïdes totaux – Activité antioxydante Evaluation of the Antioxidant Activity of Berberis vulgaris L. Abstract: Natural substances from plants have multiple inte-rests exploited in biotechnology industry both in food, cosme-tic and pharmaceutical. These compounds include a large pro-portion of secondary metabolites that are illustrated in many areas and even in therapy. In this context, we investigated the antioxidant activity of Berberis vulgaris, a plant used in Algeria, and it is particularly used in the Tlemcen region. After prepa-ration of extracts from the root bark of Berberis vulgaris, total alkaloids were analyzed by thin layer chromatography, indica-ting the presence of six tasks. A quantitative analysis of poly-phenols and flavonoids in methanolic extract was carried out, showing a content of 10.48 mg GAE/g of total polyphenols and 2.05 mg CEQ/g of flavonoids. The antioxidant activity of hydromethanolic extracts under reflux and total alkaloids was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC 50 of 1.40 mg/ml.
The phytochemical screening, the antihyperglycemic and antihyperlipidemic effects of crude hydroa... more The phytochemical screening, the antihyperglycemic and antihyperlipidemic effects of crude hydroalcoholic extract of aerial parts of Marrubium vulgare L. were evaluated in normal and Streptozotocin-induced diabetic in Wistar rats. First, the preliminary phytochemical test to identify the chemical constituents of crude hydroalcoholic extract of aerial parts of M. vulgare was carried. Next, the rats were treated with a single intraperitoneal injection dose of 300 mg/kg body weight. The changes in fasting blood glucose level were measured, in the short term, before (0, 60, 120 and 180min) after the treatments. After 14 days experimental period, rats were weighed and blood was collected for measurement of serum glucose, total cholesterol, and triglycerides. The phytochemical investigation of M. vulgare led to the characterization of several families of secondary metabolites: flavonoids, coumarins, saponins, tannins, terpenes and/or sterols. The statistical data indicated the very significant (p<0.0001) decrease in the fasting blood glucose, total cholesterol and triglycerides, for diabetic rats, 2 weeks after injection of extract studied, compared to control diabetic rat. This decrease is about 61%, 26% and 15%, respectively, compared to initial value day in which the treatment commenced. The hydroalcoholic extract of aerial parts of M. vulgare L. has had beneficial effects in reducing the elevated blood glucose level and lipid profile of STZ-induced-diabetic rats.
The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following ph... more The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following physiological and immunological changes: hyperglycemia and hypercholesterolemia involving mainly the free fraction of cholesterol, with an elevation of high-density-lipoprotein levels and a decrease in B and T splenic lymphocyte proliferation in the presence of different mitogens PHA-P, Con A and LPS. These results demonstrate the important modification that could be induced in sand rat by the standard laboratory diet as compared with natural diet, and thus the sand rat (P. obesus) appears to be an interesting model for studies on experimental diabetes mellitus.
Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus... more Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus. Intra peritoneal admin-istration of the aqueous extract of the seed (1.25 g/kg body weight) to streptozotocin-induced diabetic rats produced a significant reduction of blood sugar level in the long term, while the same extract produced no alteration of glycaemia in normal rats in the short term. The aqueous extract of this plant seems to have maximal adverse effect and high LD100 value.
Laboratoire antibiotiques, antifongiques : physicochimie, synthèse et activité biologique, facult... more Laboratoire antibiotiques, antifongiques : physicochimie, synthèse et activité biologique, faculté SNV-STU, université Abou-Bekr-Belkaïd, BP 119, Tlemcen, Algérie Correspondance : [email protected] was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC 50 of 1.40 mg/ml.
ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology in... more ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology industries both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites which are illustrated in many areas and even in therapy. We investigated the antimicrobial effects ofBerberis vulgaris, a plant used in Algeria, and particularly in Tlemcen region. The qualitative phytochemical examination performed onBerberis vulgaris root bark showed the presence of alkaloids, tannins, sterols, triterpenes and reducing compounds in large quantities, and it also revealed lower amounts of coumarins, terpenoids, saponins and mucilage. The detection of these chemical classes is followed by extraction using solvents with increasing polarity and extraction of total alkaloids. Then, the different extracts were tested for their antimicrobial potency on different reference strains of bacteria and yeasts, using the agar diffusion technique and the determination of minimum inhibitory concentration MIC. The methanolic macerated extract had the best antibacterial activity with inhibition diameters from 12.5 to 21 mm for some strains. Also,extracts of Berberis vulgarisand more specifically alkaloids showed a strong antifungal activity onCandida albicans yeasts (diameters inhibition 19 to 29 mm)
ophthalmic solution. In double RO water, MOX showed maximum absorbance at 288.2 nm. In method I, ... more ophthalmic solution. In double RO water, MOX showed maximum absorbance at 288.2 nm. In method I, AUC between two wavelengths 279.0 nm and 296.4 nm were selected. In Method II, UV-spectrum of MOX was derivatized into first order and two wavelengths 289.4 nm and 305.6 nm were selected for determination of AUC. In both these methods, MOX obeyed linearity in the concentration range of 2 − − − − 12 µg/mL (r 2 > 0.99). Proposed methods were applied for ophthalmic solution and amounts of MOX estimated by method I and method II were found to be 99.97 ± 1.21 and 99.86 ± 1.82, respectively. Both these methods were validated statistically and by recovery experiments.
Objective: Different dry extracts of Memecylon umbellatum Burm leaf obtained by various solvents ... more Objective: Different dry extracts of Memecylon umbellatum Burm leaf obtained by various solvents such as petroleum ether, chloroform, ethyl acetate, acetone, methanol and chloroform water (IP) was screened to reap the benefits of its antioxidant and free radical scavenging properties using ascorbic acid as standard antioxidants. Methods: The in vitro free radical scavenging activity was evaluated using diphenyl picryl hydrazyl (DPPH) radical method using various concentrations of dry extract in distilled water , 20 μg/ml) against blank with ascorbic acid as a standard in same concentrations. Results: Among the all extracts, Methanol leaf extract has showed higher Antioxidant activity (84.65 ± 0.064 %) having IC 50 Value 11.81 ± 0.033 μg/ml at 20 μg/ml. While, IC 50 value for ascorbic acid was found to be 8.91 ± 0.084 μg/ml. Conclusion: The results clearly indicate that Methanol leaf extract of Memecylon umbellatum is effective in free radical scavenging. So in future, this may emerge as promising natural herbal source of powerful antioxidant.
Background: Citrullus colocynthis Schrad, also known as colocynth, is a very common fruit in trad... more Background: Citrullus colocynthis Schrad, also known as colocynth, is a very common fruit in traditional medicine; it is recognized by different pharmacologic activities in traditional Algerian medicine (i.e., purgative, anti-in ammatory, antidiabetic, analgesic, and antiepileptic. Some of these activities were con rmed in modern phytotherapy (Shaheen, et al., 2014). Several scienti c studies carried out on crude extracts of pulp and seed of colocynth have demonstrated the
Postprandial hyperglycemia plays an important role in the development of type 2 diabetes and has ... more Postprandial hyperglycemia plays an important role in the development of type 2 diabetes and has been proposed as an independent risk factor for cardiovascular diseases. Herbal medicine has been used for many years by different cultures around the world for the treatment of diabetes. Punica granatum is one of the traditional remedies used for that reason. In this study, we investigated the effects of organic extracts (ethyl acetate and n-butanol) from Punica granatum peel on postprandial hyperglycemia in vivo caused by glucose, sucrose, maltose, or starch loading. Oral administration of Punica granatum peel extracts (400 mg/kg b.w.) markedly lowered plasma glucose levels in fasted normal rats. We examined in vitro whether the organic fractions of Punica granatum peel have an antidiabetic effect through α-amylase inhibitory action. Ethyl acetate and n-butanol fractions of aqueous/acetone crude extracts displayed a potent inhibitory effect on α-amylase activity (IC50 = 0.85 and 2.13 g...
Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus... more Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus. Intra peritoneal admin-istration of the aqueous extract of the seed (1.25 g/kg body weight) to streptozotocin-induced diabetic rats produced a significant reduction of blood sugar level in the long term, while the same extract produced no alteration of glycaemia in normal rats in the short term. The aqueous extract of this plant seems to have maximal adverse effect and high LD100 value.
Introduction: Citrullus colocynthis Schrad, conjointly referred to as colocynth, could be a quite... more Introduction: Citrullus colocynthis Schrad, conjointly referred to as colocynth, could be a quite common fruit in ancient drugs; it's recognized by totally different medical specialty activities in ancient Algerian medicine (i.e., purgative, medicinal drug, medicament, analgesic, and medicine. a number of these activities were confirmed in trendy herbal therapy many scientific studies applied on crude extracts of pulp and seed of colocynth have incontestable the antimitotic impact medicamen antiparasitic larvicidal moreover as a preventive impact against fatness. However, the bioactive chemicals compounds accountable of these activities don't seem to be isolated and known nonetheless. This is perennial herbs sometimes trailing, usually found wild within the sandy lands of North West, the Punjab, Sind, and Central and southern Asian nation, and coromandal coast. conjointly found autochthonal in peninsula, West Asia, and Tropical continent and within the Mediterranean region. ...
This study was undertaken to evaluate the correlation between total phenolic and flavonoid conten... more This study was undertaken to evaluate the correlation between total phenolic and flavonoid contents and antioxidant activity of various extracts of Punica granatum peel using ferric reducing antioxidant power (FRAP) and radical scavenging methods (DPPH). Preliminary phytochemical screening shows that the major constituents of total extracts are tannins and flavonoids. Phenolic and flavonoid contents were determined using spectrophotometric methods of hydromethanolic and aqueous-acetone crude extracts and their fractions, showing a high content in ethyl acetate fractions of each extract (597.08 ± 3.9 and 602.8 ± 0.0 mg GAE/gE respectively) followed by n-butanol fractions of each extract (413.6 ± 1.48 and 417.56 ± 5.3 mg EQC/gE respectively). Crude extracts showed the lower contents than the ethyl acetate and n-butanol fractions. FRAP method revealed that the ethyl acetate fraction from hydromethanolic extract showed the highest power to reduce iron followed by n-butanol fraction from...
The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following ph... more The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following physiological and immunological changes: hyperglycemia and hypercholesterolemia involving mainly the free fraction of cholesterol, with an elevation of high-density-lipoprotein levels and a decrease in B and T splenic lymphocyte proliferation in the presence of different mitogens PHA-P, Con A and LPS. These results demonstrate the important modification that could be induced in sand rat by the standard laboratory diet as compared with natural diet, and thus the sand rat (P. obesus) appears to be an interesting model for studies on experimental diabetes mellitus.
1 Laboratoire d'antibiotiques et antifongiques : physico-chimie, synthèse et activités biolog... more 1 Laboratoire d'antibiotiques et antifongiques : physico-chimie, synthèse et activités biologiques, Département de biologie moléculaire et cellulaire, Faculté des Sciences de la nature et de la vie, de la terre et de l'univers, BP119, Université Abou bekr Belkaïd, Tlemcen 13000, Algérie. 2 Laboratoire de Produits Naturels, département de biologie moléculaire et cellulaire, Faculté des Sciences de la nature et de la vie, de la terre et de l'univers, BP119, Université Abou bekr Belkaïd, Tlemcen 13000, Algérie. ______________________________________________________________________________ ABSTRACT This study was designed to evaluate the antidiabetic effect of hydroalcoholic extract from Punica granatum peels in normal and streptozotocin-induced diabetic rats and its potent α-amylase inhibitory. Antidiabetic effect of this extract was investigated after oral administration at dose of 400 mg/kg b.w. In normal fasted rats, the test drug extract showed no hypoglycaemic effect b...
ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology in... more ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology industries both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites which are illustrated in many areas and even in therapy. We investigated the antimicrobial effects ofBerberis vulgaris, a plant used in Algeria, and particularly in Tlemcen region. The qualitative phytochemical examination performed onBerberis vulgaris root bark showed the presence of alkaloids, tannins, sterols, triterpenes and reducing compounds in large quantities, and it also revealed lower amounts of coumarins, terpenoids, saponins and mucilage. The detection of these chemical classes is followed by extraction using solvents with increasing polarity and extraction of total alkaloids. Then, the different extracts were tested for their antimicrobial potency on different reference strains of bacteria and yeasts, using the agar diffusion technique and the determination of minimum inhibitory concentration MIC. The methanolic macerated extract had the best antibacterial activity with inhibition diameters from 12.5 to 21 mm for some strains. Also,extracts of Berberis vulgarisand more specifically alkaloids showed a strong antifungal activity onCandida albicans yeasts (diameters inhibition 19 to 29 mm)
ABSTRACT Pharmacognosie Évaluation de l&amp;#39;activité antioxydante de Berberis vulgaris L.... more ABSTRACT Pharmacognosie Évaluation de l&amp;#39;activité antioxydante de Berberis vulgaris L. Résumé : Les substances naturelles issues des végétaux présen-tent des intérêts multiples mis à profit dans la biotechnologie tant dans l&amp;#39;industrie alimentaire, cosmétique que pharmaceu-tique. Parmi ces composés, on retrouve une grande partie des métabolites secondaires qui se sont illustrés dans beaucoup de domaines et même en thérapie. Dans ce contexte, nous avons recherché l&amp;#39;activité antioxydante de Berberis vulgaris, une plante utilisée en Algérie et plus particulièrement dans la région de Tlemcen. Après préparation des extraits à partir des écorces de racines de Berberis vulgaris, les alcaloïdes totaux ont été analysés par chromatographie sur couche mince, révélant la présence de six taches. Aussi, une analyse quantitative des polyphénols et des flavonoïdes de l&amp;#39;extrait hydrométhanolique a été réalisée, montrant une teneur de 10,48 mg GAE/g en polyphénols totaux et 2,05 mg CEQ/g en flavonoïdes. L&amp;#39;activité antioxydante des extraits hydrométhanolique et alcaloïdes totaux a été évaluée par deux méthodes : la réduction du fer et le piégeage du radical libre DPPH. Les résultats obtenus ont montré une activité élevée de l&amp;#39;extrait hydrométhanolique par rapport aux alcaloïdes totaux, avec une CI 50 de 1,40 mg/ml. Mots clés : Berberis vulgaris – Extrait hydrométhanolique – Alcaloïdes totaux – Activité antioxydante Evaluation of the Antioxidant Activity of Berberis vulgaris L. Abstract: Natural substances from plants have multiple inte-rests exploited in biotechnology industry both in food, cosme-tic and pharmaceutical. These compounds include a large pro-portion of secondary metabolites that are illustrated in many areas and even in therapy. In this context, we investigated the antioxidant activity of Berberis vulgaris, a plant used in Algeria, and it is particularly used in the Tlemcen region. After prepa-ration of extracts from the root bark of Berberis vulgaris, total alkaloids were analyzed by thin layer chromatography, indica-ting the presence of six tasks. A quantitative analysis of poly-phenols and flavonoids in methanolic extract was carried out, showing a content of 10.48 mg GAE/g of total polyphenols and 2.05 mg CEQ/g of flavonoids. The antioxidant activity of hydromethanolic extracts under reflux and total alkaloids was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC 50 of 1.40 mg/ml.
The phytochemical screening, the antihyperglycemic and antihyperlipidemic effects of crude hydroa... more The phytochemical screening, the antihyperglycemic and antihyperlipidemic effects of crude hydroalcoholic extract of aerial parts of Marrubium vulgare L. were evaluated in normal and Streptozotocin-induced diabetic in Wistar rats. First, the preliminary phytochemical test to identify the chemical constituents of crude hydroalcoholic extract of aerial parts of M. vulgare was carried. Next, the rats were treated with a single intraperitoneal injection dose of 300 mg/kg body weight. The changes in fasting blood glucose level were measured, in the short term, before (0, 60, 120 and 180min) after the treatments. After 14 days experimental period, rats were weighed and blood was collected for measurement of serum glucose, total cholesterol, and triglycerides. The phytochemical investigation of M. vulgare led to the characterization of several families of secondary metabolites: flavonoids, coumarins, saponins, tannins, terpenes and/or sterols. The statistical data indicated the very significant (p<0.0001) decrease in the fasting blood glucose, total cholesterol and triglycerides, for diabetic rats, 2 weeks after injection of extract studied, compared to control diabetic rat. This decrease is about 61%, 26% and 15%, respectively, compared to initial value day in which the treatment commenced. The hydroalcoholic extract of aerial parts of M. vulgare L. has had beneficial effects in reducing the elevated blood glucose level and lipid profile of STZ-induced-diabetic rats.
The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following ph... more The standard laboratory diet administered to sand rat (Psammomys obesus) induces the following physiological and immunological changes: hyperglycemia and hypercholesterolemia involving mainly the free fraction of cholesterol, with an elevation of high-density-lipoprotein levels and a decrease in B and T splenic lymphocyte proliferation in the presence of different mitogens PHA-P, Con A and LPS. These results demonstrate the important modification that could be induced in sand rat by the standard laboratory diet as compared with natural diet, and thus the sand rat (P. obesus) appears to be an interesting model for studies on experimental diabetes mellitus.
Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus... more Citrullus colocinthis L. is used in Algerian folk medecine for the treatment of diabetes mellitus. Intra peritoneal admin-istration of the aqueous extract of the seed (1.25 g/kg body weight) to streptozotocin-induced diabetic rats produced a significant reduction of blood sugar level in the long term, while the same extract produced no alteration of glycaemia in normal rats in the short term. The aqueous extract of this plant seems to have maximal adverse effect and high LD100 value.
Laboratoire antibiotiques, antifongiques : physicochimie, synthèse et activité biologique, facult... more Laboratoire antibiotiques, antifongiques : physicochimie, synthèse et activité biologique, faculté SNV-STU, université Abou-Bekr-Belkaïd, BP 119, Tlemcen, Algérie Correspondance : [email protected] was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC 50 of 1.40 mg/ml.
ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology in... more ABSTRACT Natural substances from plants have multiple interests exploited in the biotechnology industries both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites which are illustrated in many areas and even in therapy. We investigated the antimicrobial effects ofBerberis vulgaris, a plant used in Algeria, and particularly in Tlemcen region. The qualitative phytochemical examination performed onBerberis vulgaris root bark showed the presence of alkaloids, tannins, sterols, triterpenes and reducing compounds in large quantities, and it also revealed lower amounts of coumarins, terpenoids, saponins and mucilage. The detection of these chemical classes is followed by extraction using solvents with increasing polarity and extraction of total alkaloids. Then, the different extracts were tested for their antimicrobial potency on different reference strains of bacteria and yeasts, using the agar diffusion technique and the determination of minimum inhibitory concentration MIC. The methanolic macerated extract had the best antibacterial activity with inhibition diameters from 12.5 to 21 mm for some strains. Also,extracts of Berberis vulgarisand more specifically alkaloids showed a strong antifungal activity onCandida albicans yeasts (diameters inhibition 19 to 29 mm)
ophthalmic solution. In double RO water, MOX showed maximum absorbance at 288.2 nm. In method I, ... more ophthalmic solution. In double RO water, MOX showed maximum absorbance at 288.2 nm. In method I, AUC between two wavelengths 279.0 nm and 296.4 nm were selected. In Method II, UV-spectrum of MOX was derivatized into first order and two wavelengths 289.4 nm and 305.6 nm were selected for determination of AUC. In both these methods, MOX obeyed linearity in the concentration range of 2 − − − − 12 µg/mL (r 2 > 0.99). Proposed methods were applied for ophthalmic solution and amounts of MOX estimated by method I and method II were found to be 99.97 ± 1.21 and 99.86 ± 1.82, respectively. Both these methods were validated statistically and by recovery experiments.
Objective: Different dry extracts of Memecylon umbellatum Burm leaf obtained by various solvents ... more Objective: Different dry extracts of Memecylon umbellatum Burm leaf obtained by various solvents such as petroleum ether, chloroform, ethyl acetate, acetone, methanol and chloroform water (IP) was screened to reap the benefits of its antioxidant and free radical scavenging properties using ascorbic acid as standard antioxidants. Methods: The in vitro free radical scavenging activity was evaluated using diphenyl picryl hydrazyl (DPPH) radical method using various concentrations of dry extract in distilled water , 20 μg/ml) against blank with ascorbic acid as a standard in same concentrations. Results: Among the all extracts, Methanol leaf extract has showed higher Antioxidant activity (84.65 ± 0.064 %) having IC 50 Value 11.81 ± 0.033 μg/ml at 20 μg/ml. While, IC 50 value for ascorbic acid was found to be 8.91 ± 0.084 μg/ml. Conclusion: The results clearly indicate that Methanol leaf extract of Memecylon umbellatum is effective in free radical scavenging. So in future, this may emerge as promising natural herbal source of powerful antioxidant.
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