Papers by Dwaipayan Sikdar
Food Research, Dec 12, 2021
Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus ... more Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.
BioTechnologia, 2019
Insect guts, particularly of phytophagous insects, are considered as intriguing bioprospecting so... more Insect guts, particularly of phytophagous insects, are considered as intriguing bioprospecting sources of cellulase and xylanase due to their use in biofuel industry. In this study, the activities of cellulase and xylanase were identified in the gut fluid of grasshopper, Oxya velox, and characterized. Qualitative assays of gut fluid carried out for endo-β-1,4-D-glucanase (EC 3.2.1.4) and endo-β-1,4-D-xylanase (EC 3.2.1.8) activities, using substrate-agar plate method, revealed clear transparent zones against the red-stained background. When measured by dinitrosalicylic acid method, the gut fluid had 0.759 ± 0.005 U and 0.303 ± 0.002 U of endoglucanase and endoxylanase activities, respectively, per mg of protein. In the gel zymogram, four distinct cellulolytic protein bands and one xylanolytic protein band were detected against substrates carboxymethyl cellulose and xylan, respectively. The optimal temperature and pH of both endo-β-1,4-D-glucanase and endo-β-1,4-D-xylanase were 55EC and 5, respectively. Preincubation at 70EC for 20 min resulted in almost complete loss of endo-β-1,4-D-glucanase activity as shown by zymography. The presence of both cellulase and xylanase activities suggested that O. velox could be considered as a model for studying the process of lignocellulose digestion in insect gut; the insect could also serve as a good source of enzymes for biofuel production.
Research Journal of Dairy Sciences, 2010
Journal of Bio-Science, 2011
Context: Racial and sex differences are documented globally regarding patterns of serum lipids am... more Context: Racial and sex differences are documented globally regarding patterns of serum lipids among diabetic patients. Differences of lipid profile of normal and diabetic subjects of the different ethnic groups of Bangladesh may differ.Objectives: To find out if there were any differences in the serum lipid and lipoprotein levels in the type 2 diabetic patients of two different races namely, Bangalee and Chakma of Bangladesh. Materials and Methods: Study population consisted of 76 (Chakma / Bangalee: 35 / 41) patients aged between 40-60 years with type 2 diabetes mellitus who were on follow-up at the Rangamati Diabetes Hospital, Chittagong Hill Tract, Bangladesh and forty (Chakma / Bangalee: 20 / 20) non-diabetic subjects of same age were randomly selected as control. Biochemical parameters analyzed include - Serum total cholesterol and triglyceride (TG), serum high-density lipoprotein (HDL), low-density lipoprotein (LDL) cholesterol and atherogenic index.Results: Dyslipidemia was...
Pakistan Journal of Biological Sciences, 2012
South African Journal of Diabetes and Vascular Disease, 2012
Objective : Epidemiological evidence suggests that levels of inflammatory markers, such as tumour... more Objective : Epidemiological evidence suggests that levels of inflammatory markers, such as tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), can predict the development of diabetes but the association of these inflammatory markers with the basic pathology of type 2 diabetes is unclear. In the present study we investigated the association of TNF-α and IL-6 with insulin secretion and insulin sensitivity in subjects with type 2 diabetes. Methods : Thirty-seven subjects with type 2 diabetes were studied along with 56 age- and body mass index (BMI)-matched controls. Insulin was measured by ELISA analysis and TNF-α and IL-6 chemiluminescence-based EIA. Results : Fasting serum TNF-α and IL-6 levels were significantly higher in subjects with type 2 diabetes (p = 0.032) than in controls. In binary logistic regression analysis, TNF-α was significantly (p = 0.026) associated with diabetes when age, gender and BMI were matched. In linear multiple regression analysis, it was found that ...
International journal of current research and review, 2013
Canadian Chemical Transactions
Efficient and low-cost cellulolytic enzymes are urgently needed to degrade recalcitrant plant bio... more Efficient and low-cost cellulolytic enzymes are urgently needed to degrade recalcitrant plant biomass during the industrial production of biofuels. Nevertheless, Blepharomastix is a serious pest of okra plant as it feeds on the leaves, soft stems, fruit of okra and eventually damage okra production. Therefore, the aim of this study is to characterize the total cellulase and endo-β-1, 4-glucanase (digestive enzymes) present in the larvae of Blepharomastix as it is a popular trend to apply enzymes in biofuels production and application of enzyme inhibitors to protect crops from damaging throughout the world. The result analysis showed that the whole gut from the larvae exhibited the activities of total cellulase and endo-β-1, 4-glucanase enzymes that hydrolyzed crystalline cellulose and carboxymethyle cellulose (CMC) to glucose and the activities in insect were 0.076 µmol min-1 ml-1 and 0.398 µmol min-1 ml-1 respectively. However, the optimum temperature for the activity of total cellulase and endo-β-1, 4-glucanase in Blepharomastix were 45˚C and 50˚C respectively. The pH optima of total cellulase and endo-β-1, 4-glucanase in Blepharomastix was 9.0. The cellulase activity was inhibited by ethylenediaminetetraaceticacid (EDTA), sodium dodecyl sulphate (SDS) and urea, whereas enhanced by NaCl, KCl and MgCl2.
ABSTRACT Background: Racial differences in serum lipid and lipoprotein levels exist and assumed t... more ABSTRACT Background: Racial differences in serum lipid and lipoprotein levels exist and assumed to be important determinants of cardiovascular disease. The purpose of this study is to investigate if there are any differences in serum lipid and lipoprotein level in the population of two different races of Bangladesh. Materials and Methods: Ninty one Chakma subjects (43 male and 48 female) and equal number of Bangalee subjects (39 male and 52 female) were randomly selected. Serum Total Cholesterol (TC), Triglyceride (TG), High Density Lipoprotein Cholesterol (HDL-C) of the study subjects were measured using commercial kits and Low Density Lipoprotein Cholesterol (LDL-C) was calculated. Result: The Bangalee subjects had higher levels of TC, TG, LDL-C and lower level of HDL-C than the Chakma population. The Bangalee males had higher TC, TG and LDL-C than the Chakma male. No significant difference was observed for HDL-C. Bangalee female had higher TC and TG and lower HDL-C than the Chakma female. No significant difference was found for LDL-C. Conclusion: This study clearly showed that racial variations exist in serum lipid and lipoproteins levels between the Bangalee and Chakma population of Bangladesh.
International Food Research Journal
Phenolic content, antioxidant and anti-amylase activities were studied in the ethanolic extract o... more Phenolic content, antioxidant and anti-amylase activities were studied in the ethanolic extract of five available Bangladeshi fruits. The fruits were Averrhoa bilimbi (Bilimbi), Artocarpus lacucha (Monkey jack), Cucumis melo (Mask melon), Phoenix sylvestris (Wild date palm) and Flacourita jangomas (Indian plum). P. sylvestris had the highest total phenolic content (37.40 ± 1.72 mg GAE/10 g of extract), whereas C. melo had the lowest (6.02 ± 0.89 mg GAE/10 g of extract). All the fruits showed DPPH free radical scavenging activity with the IC50 values for P. sylvestris, A. lacucha, F. jangomas, C. melo and A. bilimbi were 1.90 µg/ml, 0.798 mg/ml, 1.144 mg/ml, 1.695 mg/ml and 3.683 mg/ml respectively. Highest level of reducing activity was found in P. sylvestris (O.D. 0.933 ± 0.02) and reducing power activity was lowest in A. bilimbi (O.D. 0.249 ± 0.01) at a concentration of 0.4 mg/ml. But all the fruits showed a dose-dependent increase in reducing power. The fruit extracts showed very...
Background: Racial differences in serum lipid and lipoprotein levels exist and assumed to be impo... more Background: Racial differences in serum lipid and lipoprotein levels exist and assumed to be important determinants of cardiovascular disease. The purpose of this study is to investigate if there are any differences in serum lipid and lipoprotein level in the population of two different races of Bangladesh. Materials and Methods: Ninty one Chakma subjects (43 male and 48 female) and equal number of Bangalee subjects (39 male and 52 female) were randomly selected. Serum Total Cholesterol (TC), Triglyceride (TG), High Density Lipoprotein Cholesterol (HDL-C) of the study subjects were measured using commercial kits and Low Density Lipoprotein Cholesterol (LDL-C) was calculated. Result: The Bangalee subjects had higher levels of TC, TG, LDL-C and lower level of HDL-C than the Chakma population. The Bangalee males had higher TC, TG and LDL-C than the Chakma male. No significant difference was observed for HDL-C. Bangalee female had higher TC and TG and lower HDL-C than the Chakma female....
Biotechnologia, 2019
Insect guts, particularly of phytophagous insects, are considered as intriguing bioprospecting so... more Insect guts, particularly of phytophagous insects, are considered as intriguing bioprospecting sources of cellulase and xylanase due to their use in biofuel industry. In this study, the activities of cellulase and xylanase were identified in the gut fluid of grasshopper, Oxya velox, and characterized. Qualitative assays of gut fluid carried out for endo-β-1,4-D-glucanase (EC 3.2.1.4) and endo-β-1,4-D-xylanase (EC 3.2.1.8) activities, using substrate-agar plate method, revealed clear transparent zones against the red-stained background. When measured by dinitrosali-cylic acid method, the gut fluid had 0.759 ± 0.005 U and 0.303 ± 0.002 U of endoglucanase and endoxylanase activities , respectively, per mg of protein. In the gel zymogram, four distinct cellulolytic protein bands and one xylano-lytic protein band were detected against substrates carboxymethyl cellulose and xylan, respectively. The optimal temperature and pH of both endo-β-1,4-D-glucanase and endo-β-1,4-D-xylanase were 55EC and 5, respectively. Pre-incubation at 70EC for 20 min resulted in almost complete loss of endo-β-1,4-D-glucanase activity as shown by zymography. The presence of both cellulase and xylanase activities suggested that O. velox could be considered as a model for studying the process of lignocellulose digestion in insect gut; the insect could also serve as a good source of enzymes for biofuel production.
Objective: Epidemiological evidence suggests that levels of inflammatory markers, such as tumour ... more Objective: Epidemiological evidence suggests that levels of inflammatory markers, such as tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), can predict the development of diabetes but the association of these inflammatory markers with the basic pathology of type 2 diabetes is unclear. In the present study we investigated the association of TNF-α and IL-6 with insulin secretion and insulin sensitivity in subjects with type 2 diabetes. Methods: Thirty-seven subjects with type 2 diabetes were studied along with 56 age-and body mass index (BMI)matched controls. Insulin was measured by ELISA analysis and TNF-α and IL-6 chemiluminescence-based EIA. Results: Fasting serum TNF-α and IL-6 levels were significantly higher in subjects with type 2 diabetes (p = 0.032) than in controls. In binary logistic regression analysis, TNF-α was significantly (p = 0.026) associated with diabetes when age, gender and BMI were matched. In linear multiple regression analysis, it was found that levels of HOMA S (p = 0.010), HOMA B (p = 0.003) and fasting glucose (p = 0.004), and WHR (p = 0.017) were negatively associated with TNF-α in diabetic subjects. In the same analysis, HOMA S (p = 0.013), HOMA B (p = 0.002) and fasting glucose levels (p = 0.001), and WHR (p = 0.038) were positively associated with IL-6 in diabetic subjects. The association of TNF-α was positive with IL-6 (p = 0.001). Conclusion: TNF-α and IL-6 levels were both associated with insulin resistance and insulin secretory defects in patients with type 2 diabetes, and hyperglycaemia seemed to be a major trigger for pro-inflammatory changes in diabetes.
Bangladesh Journal of Genetics and Biotechnology, 2006
Objective: Epidemiological evidence suggests that levels of inflammatory markers, such as tumour ... more Objective: Epidemiological evidence suggests that levels of inflammatory markers, such as tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), can predict the development of diabetes but the association of these inflammatory markers with the basic pathology of type 2 diabetes is unclear. In the present study we investigated the association of TNF-α and IL-6 with insulin secretion and insulin sensitivity in subjects with type 2 diabetes. Methods: Thirty-seven subjects with type 2 diabetes were studied along with 56 age- and body mass index (BMI)-matched controls. Insulin was measured by ELISA analysis and TNF-α and IL-6 chemiluminescence-based EIA. Results: Fasting serum TNF-α and IL-6 levels were significantly higher in subjects with type 2 diabetes (p = 0.032) than in controls. In binary logistic regression analysis, TNF-α was significantly (p = 0.026) associated with diabetes when age, gender and BMI were matched. In linear multiple regression analysis, it was found that lev...
Bangladesh Journal of Nutrition, 2012
Journal of Bio-Science, 2011
Context: Racial and sex differences are documented globally regarding patterns of serum lipids am... more Context: Racial and sex differences are documented globally regarding patterns of serum lipids among diabetic patients. Differences of lipid profile of normal and diabetic subjects of the different ethnic groups of Bangladesh may differ.
Pakistan Journal of Biological Sciences, 2012
ABSTRACT Endo-beta-1, 4-glucanase (EC 3.2.1.4) activity was measured in the gut fluid of phytopha... more ABSTRACT Endo-beta-1, 4-glucanase (EC 3.2.1.4) activity was measured in the gut fluid of phytophagous insect Podontia quatuordecimpunctata Linnaeus (Coleoptera: Chrysomelidae) in different days of development. The eight day-old larva showed maximum activity with 1.73 U mg(-1) of protein, which was confirmed by gel zymography. In zymogram, using Carboxymethyl Cellulose (CMC) as substrate, four distinct cellulolytic protein bands were detected in leaf borer gut fluids through out of its development. The optimum temperature and pH were 60 degrees C and 5.0, respectively. This endo-beta-1,4-glucanase showed maximum stability at 20-45 degrees C with approximately 20% remaining activity. Zymography also showed complete loss of endo-beta-1,4 glucanase activity at 55 degrees C. This is the first report that the cellulolytic enzyme is produced in the gut of P. quatuordecimpunctata through the whole developmental stages, from the 1st instar to the adult, except for pupae.
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Papers by Dwaipayan Sikdar