Papers by Antônio Paulino
The production of antagonistic substance by bacterium present in the infected root canal system (... more The production of antagonistic substance by bacterium present in the infected root canal system (RCS) probably is an important ecological factor for its successful colonization of the local. The objective of this study was to partially characterize an antagonistic substance produced by a Clostridium butyricum isolated from infected RCS. Production of inhibitory compound was evaluated by the agar double layer diffusion technique using Fusobacterium nucleatum and Bifidobacterium adolescentis as indicator bacteria. The physicochemical and biochemical factors tested for the partial characterization were influence of pH and temperature and susceptibility to the action of some proteolytic enzymes. An inhibition zone was observed against the two indicator strains and acidity and bacteriophage were rejected as responsible for this phenomenon. The inhibitory activity showed to be decreasing in a pH range from 3.5 to 6.5 and being stable at temperatures of 60º, 70º and 100ºC, but completely i...
Objective: The aim of this report was to describe challenging cases of modern endodontic microsur... more Objective: The aim of this report was to describe challenging cases of modern endodontic microsurgery in teeth with persistent periodontitis following previous endodontic treatment. Methods: Four challenging cases of modern endodontic microsurgery were evaluated. Results: Healing has occurred at 6 and 12-month review appointments. Conclusion: The removal of the contamination and satisfactory sealing of the root canal system were paramount for the success observed in these cases. Modern microsurgery protocol with the use of a magnified view, ultrasonic tips and biocompatible materials are an important component of endodontic surgery, improving the success when compared to traditional technique.
Pulp canal obliteration (PCO) is defined as a deposition of hard tissue within the root canal spa... more Pulp canal obliteration (PCO) is defined as a deposition of hard tissue within the root canal space. These tissues can eventually produce the radiographic appearance of a root canal space that has become partial or completely calcified. Success in root canal treatment is based on proper debridement, disinfection and obturation of the root canal system. However, this procedure may be difficult or even impossible to achieve if the pulpal space is calcified. The endodontic treatment performed under these circumstances pose the risk of root perforating, a complication that seriously affects the long-term prognosis of tooth. The present article discusses a series of cases of endodontic treatment in teeth with partially or completely PCO and methods for the clinical management of these cases using contemporary endodontic techniques.
Journal of Endodontics, 2008
This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune response... more This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune responses. BALB/c mice were immunized with heat-killed Fusobacterium nucleatum (Fn) in MTA or other control adjuvants, and serum IgG responses to Fn were measured. Either Fn-or Peptostreptococcus anaerobius (Pa)-reactive memory T cells (Tm) were pre-incubated in vitro with/ without MTA and restimulated with Fn or Pa. Tm proliferation and cytokine production were assessed. Compared to control groups, IgG-antibody responses were upregulated in mice immunized with Fn in MTA in a similar manner to animals immunized with Fn in Freund's adjuvant or aluminum hydroxide adjuvant. While MTA did not affect the upregulated expression of IL-10, TNF-α or RANKL by Tm, it suppressed the proliferation of Pa-or Fn-Tm and inhibited their production of Th1-or Th2-signature cytokines. MTA upregulated the adaptive humoral immune responses, but had little or no effect on pro-or anti-inflammatory cytokine production by Tm.
The objective of this study was to determine the ecological relationships between bacterial speci... more The objective of this study was to determine the ecological relationships between bacterial species that colonize infected root canals. Root canal bacteria recovered from one patient with pulp canal necrosis were evaluated in vitro for synergistic and antagonistic activities determined by mono and co-culture growth kinetics and the production of bacteriocin-like substances using the double layer diffusion method. Peptostreptococcus prevotii triggered a significant increase of Fusobacterium nucleatum growth, while the former bacteria did not affect the growth of P. prevotii. The bacterial species did not produce antagonism activity against itself or against any of the other two species. Despite many studies have demonstrated the capability of root canal microorganisms to produce antagonistic substances, these in vitro experimental tests show the synergistic effect of P. prevotii on the growth of F. nucleatum.
This account covers the synthesis and biological applications of organoseleno-and organotelluro-b... more This account covers the synthesis and biological applications of organoseleno-and organotelluro-based molecular dyes. The most relevant applications for photodynamic therapy (PDT), fluorescent probes for the detection of endogenous reactive oxygen species (ROS), natural bioreductors like glutathione (GSH) and metals present in physiological systems will be discussed. This manuscript covers only dyes bearing small selenium-and tellurium-containing heterocycles.
Journal of Endodontics, 2008
This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune response... more This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune responses. BALB/c mice were immunized with heat-killed Fusobacterium nucleatum (Fn) in MTA or other control adjuvants, and serum IgG responses to Fn were measured. Either Fn-or Peptostreptococcus anaerobius (Pa)-reactive memory T cells (Tm) were pre-incubated in vitro with/ without MTA and restimulated with Fn or Pa. Tm proliferation and cytokine production were assessed. Compared to control groups, IgG-antibody responses were upregulated in mice immunized with Fn in MTA in a similar manner to animals immunized with Fn in Freund's adjuvant or aluminum hydroxide adjuvant. While MTA did not affect the upregulated expression of IL-10, TNF-α or RANKL by Tm, it suppressed the proliferation of Pa-or Fn-Tm and inhibited their production of Th1-or Th2-signature cytokines. MTA upregulated the adaptive humoral immune responses, but had little or no effect on pro-or anti-inflammatory cytokine production by Tm.
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Papers by Antônio Paulino