O termo microbiota intestinal refere-se ao ecossistema essencialmente bacteriano que reside norma... more O termo microbiota intestinal refere-se ao ecossistema essencialmente bacteriano que reside normalmente nos intestinos do homem. No nascimento, essa microbiota é adquirida no canal do parto, e sua composição definitiva é obtida em torno dos dois anos de idade acompanhando o homem pelo resto de sua vida. A microbiota intestinal desempenha inúmeras funções, muitas das quais somente agora começam a ser desvendadas. A sua própria composição ainda é bastante desconhecida, pois, calcula-se que pelo menos 40% das suas espécies ainda não foram cultivadas. É também conhecida como flora indígena dos intestinos, microflora intestinal ou simplesmente flora normal dos intestinos. Em 1977, redefiniu-se a microbiota intestinal, dividindo-a em duas: autóctone e alóctone. A primeira corresponde a microbiota normal ou indígena e a segunda a microbiota transitória que passa pelos intestinos, mas não o coloniza como o faz à autóctone. Como funções da microbiota intestinal pode-se citar: a resistência à colonização por outros microrganismos, a imuno-modulação e a contribuição nutricional resultante das interações locais e dos metabólitos produzidos.
Figs 7, 9, and 10 of this article [1]: • In Fig 7B, C, the control lanes are separated from the e... more Figs 7, 9, and 10 of this article [1]: • In Fig 7B, C, the control lanes are separated from the experimental lanes and it is unclear from the figure whether the results in each panel were obtained using a single blot or multiple blots. There also appear to be horizontal discontinuities above the hTR bands in Fig 7C. The original blot data underlying these results are in S1 File. The data indicated that for each experiment, the control lanes were included on the same blot as the experimental lanes, and data between the control and experimental lanes shown were omitted from the figure. For Fig 7B, the Total and Rac-GTP data were obtained using separate blots. For Fig 7C, the Rac, RhoGDI, and hTR data were obtained using different blots.
The objective of this study was to assess the probiotic potential of genuine strains of Bifidobac... more The objective of this study was to assess the probiotic potential of genuine strains of Bifidobacterium longum 51A and Weissella paramesenteroides WpK4, in experimental giardiasis.
Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug ... more Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug treatments such as probiotic microorganisms have various effects on the gastrointestinal microbiota dysbiosis and host immune system modulation. The aim of this study was to demonstrate the suitable use of Weissella paramesenteroides WpK4 strain isolated from healthy piglets as an alternative prophylactic or therapeutic treatment against Salmonella Typhimurium. Out of 37 lactic acid bacteria isolates, 24 strains belonging to the Weissella and Lactobacillus genera were analysed in vitro for desirable probiotic characteristics. The W. paramesenteroides WpK4 strain fulfilled all in vitro tests: resistance to acidic pH and bile salts, hydrophobic cell surface, antagonism against bacterial pathogens, H2O2 production and exopolysaccharide secretion, and non-transferable resistance to antibiotics. Mice fed with WpK4 showed no signs of bacterial translocation to the liver or spleen and decreased...
The effect of intestinal colonisation on the immune system was investigated in germ-free mice mon... more The effect of intestinal colonisation on the immune system was investigated in germ-free mice monoassociated with Lactobacillus strains isolated from calf faeces. Single doses of Lactobacillus acidophilus L36 or Lactobacillus salivarius L38 were administered to germ-free mice by intragastric gavage. Ten days later, the mice were euthanised. Gene expression levels of interleukin 5 (IL-5), IL-6, IL-10, IL-12b, IL-17a, gamma interferon (IFN-γ), transforming growth factor beta 1 (TGF-β1), and tumour necrosis factor alpha (TNF-α) were quantified in segments of the small and large intestines by real time quantitative polymerase chain reaction. All the mice were colonised rapidly after Lactobacillus administration with intestinal counts ranging from 6.53 to 8.26 log cfu/g. L. acidophilus L36 administration increased the expression of cytokines involved with the Th2 (IL-5, IL-6 and TGF-β1) and Th17 (IL-17a, TNF-α and IL-6) inflammatory response, whereas L. salivarius L38 appeared to stimula...
O termo microbiota intestinal refere-se ao ecossistema essencialmente bacteriano que reside norma... more O termo microbiota intestinal refere-se ao ecossistema essencialmente bacteriano que reside normalmente nos intestinos do homem. No nascimento, essa microbiota é adquirida no canal do parto, e sua composição definitiva é obtida em torno dos dois anos de idade acompanhando o homem pelo resto de sua vida. A microbiota intestinal desempenha inúmeras funções, muitas das quais somente agora começam a ser desvendadas. A sua própria composição ainda é bastante desconhecida, pois, calcula-se que pelo menos 40% das suas espécies ainda não foram cultivadas. É também conhecida como flora indígena dos intestinos, microflora intestinal ou simplesmente flora normal dos intestinos. Em 1977, redefiniu-se a microbiota intestinal, dividindo-a em duas: autóctone e alóctone. A primeira corresponde a microbiota normal ou indígena e a segunda a microbiota transitória que passa pelos intestinos, mas não o coloniza como o faz à autóctone. Como funções da microbiota intestinal pode-se citar: a resistência à colonização por outros microrganismos, a imuno-modulação e a contribuição nutricional resultante das interações locais e dos metabólitos produzidos.
Figs 7, 9, and 10 of this article [1]: • In Fig 7B, C, the control lanes are separated from the e... more Figs 7, 9, and 10 of this article [1]: • In Fig 7B, C, the control lanes are separated from the experimental lanes and it is unclear from the figure whether the results in each panel were obtained using a single blot or multiple blots. There also appear to be horizontal discontinuities above the hTR bands in Fig 7C. The original blot data underlying these results are in S1 File. The data indicated that for each experiment, the control lanes were included on the same blot as the experimental lanes, and data between the control and experimental lanes shown were omitted from the figure. For Fig 7B, the Total and Rac-GTP data were obtained using separate blots. For Fig 7C, the Rac, RhoGDI, and hTR data were obtained using different blots.
The objective of this study was to assess the probiotic potential of genuine strains of Bifidobac... more The objective of this study was to assess the probiotic potential of genuine strains of Bifidobacterium longum 51A and Weissella paramesenteroides WpK4, in experimental giardiasis.
Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug ... more Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug treatments such as probiotic microorganisms have various effects on the gastrointestinal microbiota dysbiosis and host immune system modulation. The aim of this study was to demonstrate the suitable use of Weissella paramesenteroides WpK4 strain isolated from healthy piglets as an alternative prophylactic or therapeutic treatment against Salmonella Typhimurium. Out of 37 lactic acid bacteria isolates, 24 strains belonging to the Weissella and Lactobacillus genera were analysed in vitro for desirable probiotic characteristics. The W. paramesenteroides WpK4 strain fulfilled all in vitro tests: resistance to acidic pH and bile salts, hydrophobic cell surface, antagonism against bacterial pathogens, H2O2 production and exopolysaccharide secretion, and non-transferable resistance to antibiotics. Mice fed with WpK4 showed no signs of bacterial translocation to the liver or spleen and decreased...
The effect of intestinal colonisation on the immune system was investigated in germ-free mice mon... more The effect of intestinal colonisation on the immune system was investigated in germ-free mice monoassociated with Lactobacillus strains isolated from calf faeces. Single doses of Lactobacillus acidophilus L36 or Lactobacillus salivarius L38 were administered to germ-free mice by intragastric gavage. Ten days later, the mice were euthanised. Gene expression levels of interleukin 5 (IL-5), IL-6, IL-10, IL-12b, IL-17a, gamma interferon (IFN-γ), transforming growth factor beta 1 (TGF-β1), and tumour necrosis factor alpha (TNF-α) were quantified in segments of the small and large intestines by real time quantitative polymerase chain reaction. All the mice were colonised rapidly after Lactobacillus administration with intestinal counts ranging from 6.53 to 8.26 log cfu/g. L. acidophilus L36 administration increased the expression of cytokines involved with the Th2 (IL-5, IL-6 and TGF-β1) and Th17 (IL-17a, TNF-α and IL-6) inflammatory response, whereas L. salivarius L38 appeared to stimula...
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